Asteraceae triterpenoid biosynthesis gene ataSQS and application thereof

CN122168639APending Publication Date: 2026-06-09HEBEI AGRICULTURAL UNIV.
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Patent Information

Application Number
CN202511867313.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-11
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

The regulatory mechanisms of the biosynthetic pathways of Aster triterpenoids in existing technologies are unclear, and key functional genes are lacking. This results in low efficiency and long cycles in traditional breeding methods, making it difficult to meet the market demand for high-quality Aster medicinal materials.

Method used

The biosynthesis gene AtaSQS of triterpenoids from Aster tataricus and its promoter sequence were cloned. A recombinant expression vector was constructed and the gene was overexpressed in Aster tataricus through Agrobacterium-mediated transformation. This established an efficient transgenic technology system to regulate the synthesis and accumulation of triterpenoids.

Benefits of technology

It significantly increased the content of triterpenoids in Aster tataricus, shortened the breeding cycle, improved the medicinal quality and economic value of Aster tataricus, and provided an efficient variety improvement technique.

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Abstract

This invention relates to the field of plant genetic engineering technology, and discloses an AtaSQS gene for the biosynthesis of triterpenoids in Aster tataricus and its applications. The AtaSQS gene includes a complete coding region CDS sequence and a promoter sequence, derived from *Aster tataricus* var. *ji*. The CDS sequence is shown in SEQ ID NO.1. The promoter sequence, 1289 bp in length, is located upstream of the CDS sequence and is used to regulate the spatiotemporally specific expression of the AtaSQS gene. This invention cloned the key gene AtaSQS for the biosynthesis of triterpenoids and its promoter sequence from *Aster tataricus* var. *ji*. It clarified its positive regulatory function on the synthesis of total triterpenoids and asterones, and provided related recombinant expression vectors, host bacteria, and breeding methods. This fills the technical gap in the regulatory mechanism of triterpenoid synthesis in Aster tataricus, and can significantly accelerate the breeding process of Aster tataricus varieties with high triterpenoid content, improve their medicinal quality and economic value, and has important application prospects in the fields of plant genetic engineering and the quality improvement of traditional Chinese medicinal materials.
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