Method for determining aspartate oligosaccharide content based on fingerprint and application thereof

CN122171694APending Publication Date: 2026-06-09INSTITUTE OF CHINESE MATERIA MEDICA CHINA ACADEMY OF CHINESE MEDICAL SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INSTITUTE OF CHINESE MATERIA MEDICA CHINA ACADEMY OF CHINESE MEDICAL SCIENCES
Filing Date
2024-12-06
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

Existing technologies lack effective methods to control the overall quality of Asparagus medicinal materials, especially the inability to accurately determine the content of its oligosaccharide components, making it difficult to achieve quality control of Asparagus medicinal materials, processed slices and their extracts.

Method used

A fingerprint-based method for determining oligosaccharides in asparagus was adopted, including ultrasonic extraction, liquid chromatography analysis, and similarity evaluation of chromatographic fingerprints of traditional Chinese medicine. A standard curve for oligosaccharides was established, and the quality of asparagus samples was judged by chromatographic peak matching and similarity evaluation.

Benefits of technology

It provides a more effective quality control method that can accurately determine the oligosaccharide content in Asparagus medicinal materials, improves the quality control capability of Asparagus medicinal materials, decoction pieces and their extracts, fills the gap in pharmacopoeia standards, and provides a new method for the quality evaluation of functional foods.

✦ Generated by Eureka AI based on patent content.

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Abstract

This invention discloses a method for determining asparagus oligosaccharides based on fingerprint spectroscopy and its application, relating to the field of asparagus quality evaluation technology. The method includes the following steps: adding ethanol solution to asparagus powder and sonicating to obtain a test solution; analyzing different batches of asparagus test solutions using liquid chromatography to obtain liquid chromatograms for each batch; then generating a control fingerprint spectroscopy using fingerprint spectroscopy similarity software, and evaluating similarity using the control fingerprint spectroscopy as a reference; and establishing a content determination method using oligosaccharides with a degree of polymerization of 3-10 as quantitative indicators. This invention establishes a fingerprint spectroscopy analysis method and a content determination method for asparagus oligosaccharides, providing a new method for quality control of asparagus and asparagus-containing products, effectively solving the problem that existing methods cannot effectively control the overall quality of asparagus. This method can comprehensively and accurately detect the main component oligosaccharides in asparagus, and is simple and easy to operate.
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