A composition for promoting intestinal health and a process for preparing the same

Abstract

The application provides a composition for improving intestinal health and a preparation process thereof, and belongs to the technical field of health foods, and comprises the following raw material components in mass parts: modified composite dietary fiber 50-85 parts, flaxseed 5-8 parts, pea peptide 10-15 parts, and fruit powder 5-15 parts; through step-by-step enzymolysis and a modification process, the application synchronously obtains soluble and insoluble dietary fibers with high purity and loose structure, improves the water holding capacity, oil holding capacity, swelling capacity and antioxidant activity of the composition, the prepared composition can promote intestinal peristalsis, improve intestinal health, and the composition does not add probiotics, has good stability, and is suitable for constipation people to eat.

Description

Technical Field

[0001] This invention relates to the field of health food technology, specifically to a composition for improving gut health and its preparation process. Background Technology

[0002] In recent years, with the continuous improvement of living standards, public attention to the nutritional components of food has shifted from basic energy supply to their regulatory effects on specific physiological functions. Currently, among health foods targeting gut health, directly adding probiotics is one of the mainstream solutions in the market. These products improve the balance of the host's gut microbiota by exogenously supplementing specific strains (such as Lactobacillus and Bifidobacterium). However, this approach has several inherent limitations: First, probiotics are mostly live bacteria preparations, which have relatively strict requirements for production, transportation, and storage conditions, resulting in slightly lower product stability; second, probiotics that enter the intestines orally face the challenges of the digestive environment, such as stomach acid and bile, leading to a further decrease in survival rate, and exogenous strains usually have low colonization efficiency in the host's complex native microbial community, affecting their long-term effects; more fundamentally, the composition of the human gut microbiota is highly individualized and complex, and relying solely on supplementing with a few known strains is difficult to comprehensively and accurately support the health of the overall ecological network, and its mechanism of action and effects often vary from person to person.

[0003] Based on the above issues, the "prebiotic" strategy, which provides selective substrates to promote the autonomous proliferation of existing beneficial bacteria in the gut, has gained attention due to its more universal effects, stability, and safety. Among these, dietary fiber, as one of the most important natural prebiotic sources, has become a focus of research and development.

[0004] Currently, related research and products mainly focus on utilizing or enriching soluble dietary fiber (SDF), as it can be efficiently fermented by microorganisms to produce beneficial metabolites such as short-chain fatty acids. However, this technical approach typically treats insoluble dietary fiber (IDF) as an impurity or byproduct and removes it, resulting in resource waste and neglecting the irreplaceable physical regulatory functions of IDF in maintaining normal intestinal motility and increasing fecal water retention and volume. This leads to a functional limitation in existing dietary fiber-based solutions, failing to fully realize the comprehensive health benefits of dietary fiber as a complete system.

[0005] Meanwhile, natural dietary fiber often forms bound complexes with polyphenols in plant cell walls through ester bonds, hydrogen bonds, and other means. This natural structure can endow it with synergistic effects beyond those of a single component. However, traditional SDF extraction or purification processes often employ harsh methods such as strong alkali treatment or high-temperature hydrolysis to obtain products with high solubility, which easily destroys this bond, leading to the loss of bound polyphenols and making the bioactivity of the final product tend to be singular.

[0006] Therefore, a new solution that can systematically address the above problems is urgently needed. Summary of the Invention

[0007] The purpose of this invention is to provide a composition for improving gut health and its preparation process, so as to solve the problems mentioned in the background art.

[0008] To achieve the above objectives, the present invention provides the following technical solution:

[0009] A process for preparing a composition that improves gut health includes the following steps:

[0010] S1. After drying rice bran, citrus peel and sea buckthorn pomace at 50℃, crush and sieve them as raw materials, add water and stir, bathe in a 95℃ water bath for 10 minutes, adjust the pH to 6.0, add α-amylase, enzymatically hydrolyze for 20 minutes, cool down to 60℃, adjust the pH to 7.5, add papain, enzymatically hydrolyze for 1 hour, inactivate the enzyme in a boiling water bath for 10 minutes, and cool to room temperature;

[0011] S2. Centrifuge the solution obtained in step S1, wash the precipitate twice thoroughly with distilled water at 70-80℃, freeze-dry the precipitate under vacuum to obtain insoluble fiber powder.

[0012] S3. Add 4 times the volume of 95% ethanol to the supernatant after centrifugation in step S2, let stand overnight, centrifuge, collect the precipitate, wash twice with 95% ethanol and acetone respectively, and freeze dry under vacuum to obtain soluble fiber powder.

[0013] S4. Take the insoluble fiber powder obtained in step S2, add distilled water at a liquid-to-solid ratio of 1:10 (g:mL) and stir evenly, sonicate, centrifuge, collect the precipitate and freeze dry under vacuum to obtain modified insoluble dietary fiber powder.

[0014] S5. Take the soluble fiber powder obtained in step S3, add distilled water at a liquid-to-solid ratio of 1:10 (g:mL) and stir evenly. Adjust the pH to 5.0 with 0.1 mol / L hydrochloric acid, add the compound enzyme, modify in a 50℃ water bath for 3 hours, then inactivate the enzyme in a boiling water bath for 10 minutes. After cooling to room temperature, add 4 times the volume of 95% ethanol and let stand overnight. Collect the precipitate, wash twice with 95% ethanol and acetone respectively, and freeze dry under vacuum to obtain modified soluble dietary fiber powder.

[0015] S6. Mix the modified insoluble dietary fiber powder from step S4 and the modified soluble dietary fiber powder from step S5 to obtain modified composite dietary fiber.

[0016] S7. Mix the modified composite dietary fiber, flaxseed, pea peptide, and fruit powder, pulverize, sieve, and sterilize to obtain the composition.

[0017] Preferably, in step S1, the mass ratio of rice bran, citrus peel and sea buckthorn pomace is (3~5):(1~2):1, the material-to-liquid ratio is 1:15 (g:mL), the amount of α-amylase added is 0.2% of the raw material mass, and the amount of papain added is 0.6% of the raw material mass.

[0018] Preferably, in step S4, the ultrasonic temperature is 45~55℃, the ultrasonic power is 300~400W, and the ultrasonic time is 30~40min.

[0019] Preferably, in step S5, the composite enzyme is cellulase and xylanase in a mass ratio of 1:1, and the amount of composite enzyme added is 5% of the mass of soluble fiber powder.

[0020] Preferably, in step S6, the mass ratio of modified insoluble dietary fiber powder to modified soluble dietary fiber powder is 1:(1.5~4).

[0021] Preferably, in step S7, the fruit powder is one or more of the following: monk fruit powder, hawthorn powder, kiwi fruit powder, banana powder, and dragon fruit powder.

[0022] Another aspect of the present invention discloses a composition prepared by the preparation process described in any of the above technical solutions, comprising the following raw material components by mass: 50-85 parts of modified composite dietary fiber, 5-8 parts of flaxseed, 10-15 parts of pea peptide, and 5-15 parts of fruit powder.

[0023] The beneficial effects of the above-described technical solution of the present invention are as follows:

[0024] 1. Through stepwise enzymatic hydrolysis and selective separation, high-purity insoluble dietary fiber (IDF) and soluble dietary fiber (SDF) were obtained simultaneously. After physical and enzymatic modification, the obtained IDF and SDF formed a loose and porous microstructure, which improved the water-holding capacity, oil-holding capacity and swelling capacity of dietary fiber, enhanced its physical regulatory function in the intestine (such as promoting peristalsis and increasing satiety), and also provided a superior colonization and fermentation substrate for intestinal flora.

[0025] 2. During the modification process, the natural bound state of dietary fiber and polyphenols was effectively protected through a mild initial enzymatic treatment and subsequent separation conditions. Experiments confirmed that the final product exhibited significant in vitro free radical scavenging ability, indicating that the bound polyphenols were successfully preserved and contributed antioxidant activity.

[0026] 3. The dietary fiber-polyphenol complex system obtained through mild enzymatic hydrolysis can play a synergistic role in the intestine. Polyphenols further increase the adsorption capacity of dietary fiber for lipids; dietary fiber encapsulates polyphenols, improving the stability of polyphenols in the gastrointestinal environment, thereby increasing the bioavailability of polyphenols in the intestine.

[0027] 4. A multi-layered gut health regulatory mechanism was constructed:

[0028] (1) The combination of IDF and SDF provided takes into account both the physical volume effect and the fermentation characteristics of prebiotics, and can more comprehensively support gut health.

[0029] (2) The added pea peptides are an oligopeptide source that is easy to absorb. They not only provide nutrition for intestinal cells, but their specific peptide segments also have potential functions of regulating the flora and supporting the intestinal barrier, and have a synergistic effect with dietary fiber.

[0030] (3) By adding natural fruit powder, the palatability of the product is effectively improved without relying on refined sugar or artificial sweeteners, making it more acceptable to consumers and in line with the clean label trend.

[0031] 5. The composition does not contain probiotics, and the final product is a dry powder with good stability, which makes it easy to store, transport and use, thus overcoming the defect of poor stability of probiotic preparations. Detailed Implementation

[0032] The preferred embodiments of the present invention are described below. It should be understood that the preferred embodiments described herein are for illustration and explanation only and are not intended to limit the present invention.

[0033] For experiments not specified in this protocol, the procedures and conditions described in the literature in this field should be followed. Reagents or instruments whose manufacturers are not specified are all commercially available products.

[0034] Example 1

[0035] S1. Rice bran, citrus peel and sea buckthorn pomace in a mass ratio of 4:1.5:1 were dried at 50℃, pulverized and sieved as raw materials. Water was added at a liquid-to-material ratio of 1:15 (g:mL) and stirred. The mixture was then placed in a water bath at 95℃ for 10 min. The pH was adjusted to 6.0 with 0.05 mol / L pH 4.0 acetate buffer. 0.2% α-amylase (by weight of raw materials) was added and enzymatically hydrolyzed for 20 min. The mixture was then cooled to 60℃ and the pH was adjusted to 7.5 with 0.1 mol / L pH 11 phosphate buffer. 0.6% papain (by weight of raw materials) was added and enzymatically hydrolyzed for 1 h. The enzyme was then inactivated in a boiling water bath for 10 min and cooled to room temperature.

[0036] S2. Centrifuge the solution obtained in step S1 at 4800 rpm for 20 min, wash the precipitate twice with distilled water at 80℃, freeze-dry the precipitate under vacuum to obtain insoluble fiber powder.

[0037] S3. Add 4 times the volume of 95% ethanol to the supernatant after centrifugation in step S2 and let it stand overnight. Centrifuge at 4800 rpm for 20 min, collect the precipitate, wash it twice with 95% ethanol and acetone respectively, and freeze dry it under vacuum to obtain soluble fiber powder.

[0038] S4. Take the insoluble fiber powder obtained in step S2, add distilled water at a liquid-to-solid ratio of 1:10 (g:mL) and stir evenly. The ultrasonic temperature is 50℃, the ultrasonic power is 350W, and the ultrasonic treatment lasts for 35 minutes. Centrifuge at 4800rpm for 20 minutes, collect the precipitate and freeze-dry it under vacuum to obtain modified insoluble dietary fiber powder.

[0039] S5. Take the soluble fiber powder obtained in step S3, add distilled water at a liquid-to-solid ratio of 1:10 (g:mL) and stir evenly. Adjust the pH to 5.0 with 0.1 mol / L hydrochloric acid, add cellulase and xylanase at a mass ratio of 1:1, the amount added is 5% of the mass of the soluble fiber powder, modify in a 50℃ water bath for 3 hours, then inactivate the enzymes in a boiling water bath for 10 minutes, cool to room temperature, add 4 times the volume of 95% ethanol and let stand overnight, collect the precipitate, wash twice with 95% ethanol and acetone respectively, and freeze dry under vacuum to obtain modified soluble dietary fiber powder;

[0040] S6. Mix the modified insoluble dietary fiber powder from step S4 and the modified soluble dietary fiber powder from step S5 at a mass ratio of 1:2.7 to obtain modified composite dietary fiber.

[0041] S7. Mix 70 parts of modified composite dietary fiber, 7 parts of flaxseed, 12 parts of pea peptide, 5 parts of monk fruit powder, 5 parts of hawthorn powder and 2 parts of dragon fruit powder, pulverize, sieve and sterilize to obtain the composition.

[0042] The composition provided in this solution to improve gut health is mainly intended for people with constipation. The method of use is to take 10g twice a day after meals.

[0043] Example 2

[0044] S1. Rice bran, citrus peel and sea buckthorn pomace in a mass ratio of 3:1:1 were dried at 50℃, pulverized and sieved as raw materials. Water was added at a liquid-to-material ratio of 1:15 (g:mL) and stirred. The mixture was then placed in a water bath at 95℃ for 10 min. The pH was adjusted to 6.0 with 0.05 mol / L pH 4.0 acetate buffer. 0.2% α-amylase (by weight of raw materials) was added and enzymatically hydrolyzed for 20 min. The mixture was then cooled to 60℃ and the pH was adjusted to 7.5 with 0.1 mol / L pH 11 phosphate buffer. 0.6% papain (by weight of raw materials) was added and enzymatically hydrolyzed for 1 h. The enzyme was then inactivated in a boiling water bath for 10 min and cooled to room temperature.

[0045] S2. Centrifuge the solution obtained in step S1 at 4800 rpm for 20 min, wash the precipitate twice with distilled water at 70℃, freeze-dry the precipitate under vacuum to obtain insoluble fiber powder.

[0046] S3. Add 4 times the volume of 95% ethanol to the supernatant after centrifugation in step S2 and let it stand overnight. Centrifuge at 4800 rpm for 20 min, collect the precipitate, wash it twice with 95% ethanol and acetone respectively, and freeze dry it under vacuum to obtain soluble fiber powder.

[0047] S4. Take the insoluble fiber powder obtained in step S2, add distilled water at a liquid-to-solid ratio of 1:10 (g:mL) and stir evenly. The ultrasonic temperature is 45℃, the ultrasonic power is 400W, and the ultrasonic treatment lasts for 30 minutes. Centrifuge at 4800rpm for 20 minutes, collect the precipitate and freeze dry it under vacuum to obtain modified insoluble dietary fiber powder.

[0048] S5. Take the soluble fiber powder obtained in step S3, add distilled water at a liquid-to-solid ratio of 1:10 (g:mL) and stir evenly. Adjust the pH to 5.0 with 0.1 mol / L hydrochloric acid, add cellulase and xylanase at a mass ratio of 1:1, the amount added is 5% of the mass of the soluble fiber powder, modify in a 50℃ water bath for 3 hours, then inactivate the enzymes in a boiling water bath for 10 minutes, cool to room temperature, add 4 times the volume of 95% ethanol and let stand overnight, collect the precipitate, wash twice with 95% ethanol and acetone respectively, and freeze dry under vacuum to obtain modified soluble dietary fiber powder;

[0049] S6. Mix the modified insoluble dietary fiber powder from step S4 and the modified soluble dietary fiber powder from step S5 at a mass ratio of 1:1.5 to obtain modified composite dietary fiber.

[0050] S7. Mix 50 parts of modified composite dietary fiber, 5 parts of flaxseed, 10 parts of pea peptide, 2 parts of hawthorn powder and 3 parts of banana powder, pulverize, sieve and sterilize to obtain the composition.

[0051] Example 3

[0052] S1. Rice bran, citrus peel and sea buckthorn pomace in a mass ratio of 5:2:1 were dried at 50℃, pulverized and sieved as raw materials. Water was added at a liquid-to-material ratio of 1:15 (g:mL) and stirred. The mixture was then placed in a water bath at 95℃ for 10 min. The pH was adjusted to 6.0 with 0.05 mol / L pH 4.0 acetate buffer. 0.2% α-amylase (by weight of raw materials) was added and enzymatically hydrolyzed for 20 min. The mixture was then cooled to 60℃ and the pH was adjusted to 7.5 with 0.1 mol / L pH 11 phosphate buffer. 0.6% papain (by weight of raw materials) was added and enzymatically hydrolyzed for 1 h. The enzyme was then inactivated in a boiling water bath for 10 min and cooled to room temperature.

[0053] S2. Centrifuge the solution obtained in step S1 at 4800 rpm for 20 min, wash the precipitate twice with distilled water at 80℃, freeze-dry the precipitate under vacuum to obtain insoluble fiber powder.

[0054] S3. Add 4 times the volume of 95% ethanol to the supernatant after centrifugation in step S2 and let it stand overnight. Centrifuge at 4800 rpm for 20 min, collect the precipitate, wash it twice with 95% ethanol and acetone respectively, and freeze dry it under vacuum to obtain soluble fiber powder.

[0055] S4. Take the insoluble fiber powder obtained in step S2, add distilled water at a liquid-to-solid ratio of 1:10 (g:mL) and stir evenly. The ultrasonic temperature is 55℃, the ultrasonic power is 300W, and the ultrasonic treatment lasts for 40 minutes. Centrifuge at 4800rpm for 20 minutes, collect the precipitate and freeze-dry it under vacuum to obtain modified insoluble dietary fiber powder.

[0056] S5. Take the soluble fiber powder obtained in step S3, add distilled water at a liquid-to-solid ratio of 1:10 (g:mL) and stir evenly. Adjust the pH to 5.0 with 0.1 mol / L hydrochloric acid, add cellulase and xylanase at a mass ratio of 1:1, the amount added is 5% of the mass of the soluble fiber powder, modify in a 50℃ water bath for 3 hours, then inactivate the enzymes in a boiling water bath for 10 minutes, cool to room temperature, add 4 times the volume of 95% ethanol and let stand overnight, collect the precipitate, wash twice with 95% ethanol and acetone respectively, and freeze dry under vacuum to obtain modified soluble dietary fiber powder;

[0057] S6. The modified insoluble dietary fiber powder from step S4 and the modified soluble dietary fiber powder from step S5 are mixed at a mass ratio of 1:4 to obtain modified composite dietary fiber.

[0058] S7. Mix 85 parts of modified composite dietary fiber, 8 parts of flaxseed, 15 parts of pea peptide, 5 parts of hawthorn powder, 4 parts of kiwi powder, 3 parts of banana powder and 3 parts of dragon fruit powder, pulverize, sieve and sterilize to obtain the composition.

[0059] Comparative Example 1

[0060] S1. Rice bran, citrus peel and sea buckthorn pomace in a mass ratio of 4:1.5:1 were dried at 50℃, pulverized and sieved as raw materials. Water was added at a liquid-to-material ratio of 1:15 (g:mL) and stirred. The mixture was then placed in a water bath at 95℃ for 10 min. The pH was adjusted to 6.0 with 0.05 mol / L pH 4.0 acetate buffer. 0.2% α-amylase (by weight of raw materials) was added and enzymatically hydrolyzed for 20 min. The mixture was then cooled to 60℃ and the pH was adjusted to 7.5 with 0.1 mol / L pH 11 phosphate buffer. 0.6% papain (by weight of raw materials) was added and enzymatically hydrolyzed for 1 h. The enzyme was then inactivated in a boiling water bath for 10 min and cooled to room temperature.

[0061] S2. Centrifuge the solution obtained in step S1 at 4800 rpm for 20 min, wash the precipitate twice with distilled water at 80℃, freeze-dry the precipitate under vacuum to obtain insoluble fiber powder.

[0062] S3. Add 4 times the volume of 95% ethanol to the supernatant after centrifugation in step S2 and let it stand overnight. Centrifuge at 4800 rpm for 20 min, collect the precipitate, wash it twice with 95% ethanol and acetone respectively, and freeze dry it under vacuum to obtain soluble fiber powder.

[0063] S4. Mix the insoluble fiber powder from step S2 and the soluble fiber powder from step S3 at a mass ratio of 1:2.7 to obtain composite dietary fiber;

[0064] S5. Mix 70 parts of compound dietary fiber, 7 parts of flaxseed, 12 parts of pea peptide, 5 parts of monk fruit powder, 5 parts of hawthorn powder and 2 parts of dragon fruit powder, pulverize, sieve and sterilize to obtain the composition.

[0065] Comparative Example 2

[0066] S1. Rice bran, citrus peel and sea buckthorn pomace in a mass ratio of 4:1.5:1 were dried at 50℃, pulverized and sieved as raw materials. Water was added at a liquid-to-material ratio of 1:15 (g:mL) and stirred. The mixture was then placed in a water bath at 95℃ for 10 min. The pH was adjusted to 6.0 with 0.05 mol / L pH 4.0 acetate buffer. 0.2% α-amylase (by weight of raw materials) was added and enzymatically hydrolyzed for 20 min. The mixture was then cooled to 60℃ and the pH was adjusted to 7.5 with 0.1 mol / L pH 11 phosphate buffer. 0.6% papain (by weight of raw materials) was added and enzymatically hydrolyzed for 1 h. The enzyme was then inactivated in a boiling water bath for 10 min and cooled to room temperature.

[0067] S2. Centrifuge the solution obtained in step S1 at 4800 rpm for 20 min, wash the precipitate twice with distilled water at 80℃, freeze-dry the precipitate under vacuum to obtain insoluble fiber powder.

[0068] S3. Take the insoluble fiber powder obtained in step S2, add distilled water at a liquid-to-solid ratio of 1:10 (g:mL) and stir evenly. The ultrasonic temperature is 50℃, the ultrasonic power is 350W, and the ultrasonic treatment lasts for 35 minutes. Centrifuge at 4800rpm for 20 minutes, collect the precipitate and freeze-dry it under vacuum to obtain modified insoluble dietary fiber powder.

[0069] S4. Mix 70 parts of modified insoluble dietary fiber powder, 7 parts of flaxseed, 12 parts of pea peptide, 5 parts of monk fruit powder, 5 parts of hawthorn powder and 2 parts of dragon fruit powder, pulverize, sieve and sterilize to obtain the composition.

[0070] Comparative Example 3

[0071] S1. Rice bran, citrus peel and sea buckthorn pomace in a mass ratio of 4:1.5:1 were dried at 50℃, pulverized and sieved as raw materials. Water was added at a liquid-to-material ratio of 1:15 (g:mL) and stirred. The mixture was then placed in a water bath at 95℃ for 10 min. The pH was adjusted to 6.0 with 0.05 mol / L pH 4.0 acetate buffer. 0.2% α-amylase (by weight of raw materials) was added and enzymatically hydrolyzed for 20 min. The mixture was then cooled to 60℃ and the pH was adjusted to 7.5 with 0.1 mol / L pH 11 phosphate buffer. 0.6% papain (by weight of raw materials) was added and enzymatically hydrolyzed for 1 h. The enzyme was then inactivated in a boiling water bath for 10 min and cooled to room temperature.

[0072] S2. Centrifuge the solution obtained in step S1 at 4800 rpm for 20 min, discard the precipitate, add 4 times the volume of 95% ethanol to the supernatant and let stand overnight, centrifuge at 4800 rpm for 20 min, collect the precipitate, wash twice with 95% ethanol and acetone respectively, and freeze dry under vacuum to obtain soluble fiber powder.

[0073] S3. Take the soluble fiber powder obtained in step S2, add distilled water at a liquid-to-solid ratio of 1:10 (g:mL) and stir evenly. Adjust the pH to 5.0 with 0.1 mol / L hydrochloric acid, add cellulase and xylanase at a mass ratio of 1:1, and add 5% of the mass of the soluble fiber powder. Modify in a 50℃ water bath for 3 hours, then inactivate the enzymes in a boiling water bath for 10 minutes. After cooling to room temperature, add 4 times the volume of 95% ethanol and let stand overnight. Collect the precipitate, wash twice with 95% ethanol and acetone respectively, and freeze dry under vacuum to obtain modified soluble dietary fiber powder.

[0074] S4. Mix 70 parts of modified soluble dietary fiber powder, 7 parts of flaxseed, 12 parts of pea peptide, 5 parts of monk fruit powder, 5 parts of hawthorn powder and 2 parts of dragon fruit powder, pulverize, sieve and sterilize to obtain the composition.

[0075] Comparative Example 4

[0076] S1. Rice bran, citrus peel and sea buckthorn pomace in a mass ratio of 4:1.5:1 were dried at 50℃, crushed and sieved and used as raw materials. They were added to 1mol / L HCl solution at a liquid-to-material ratio of 1:15 (g:mL) and stirred. The solution was then heated in a water bath at 70℃ for 60 min. After the solution cooled, 2mol / L NaOH was added to adjust the pH to 12. The solution was then heated in a water bath at 70℃ for 60 min. Finally, the pH was adjusted to neutral with HCl.

[0077] S2. Centrifuge the solution obtained in step S1 at 4800 rpm for 20 min, wash the precipitate twice with distilled water at 80℃, freeze-dry the precipitate under vacuum to obtain insoluble fiber powder.

[0078] S3. Add 4 times the volume of 95% ethanol to the supernatant after centrifugation in step S2 and let it stand overnight. Centrifuge at 4800 rpm for 20 min, collect the precipitate, wash it twice with 95% ethanol and acetone respectively, and freeze dry it under vacuum to obtain soluble fiber powder.

[0079] S4. Take the insoluble fiber powder obtained in step S2, add distilled water at a liquid-to-solid ratio of 1:10 (g:mL) and stir evenly. The ultrasonic temperature is 50℃, the ultrasonic power is 350W, and the ultrasonic treatment lasts for 35 minutes. Centrifuge at 4800rpm for 20 minutes, collect the precipitate and freeze-dry it under vacuum to obtain modified insoluble dietary fiber powder.

[0080] S5. Take the soluble fiber powder obtained in step S3, add distilled water at a liquid-to-solid ratio of 1:10 (g:mL) and stir evenly. Adjust the pH to 5.0 with 0.1 mol / L hydrochloric acid, add cellulase and xylanase at a mass ratio of 1:1, the amount added is 5% of the mass of the soluble fiber powder, modify in a 50℃ water bath for 3 hours, then inactivate the enzymes in a boiling water bath for 10 minutes, cool to room temperature, add 4 times the volume of 95% ethanol and let stand overnight, collect the precipitate, wash twice with 95% ethanol and acetone respectively, and freeze dry under vacuum to obtain modified soluble dietary fiber powder;

[0081] S6. Mix the modified insoluble dietary fiber powder from step S4 and the modified soluble dietary fiber powder from step S5 at a mass ratio of 1:2.7 to obtain modified composite dietary fiber.

[0082] S7. Mix 70 parts of modified composite dietary fiber, 7 parts of flaxseed, 12 parts of pea peptide, 5 parts of monk fruit powder, 5 parts of hawthorn powder and 2 parts of dragon fruit powder, pulverize, sieve and sterilize to obtain the composition.

[0083] Comparative Example 5

[0084] The difference between this and Example 1 is that no oligopeptides are added.

[0085] Experiment 1: Water-holding capacity, oil-holding capacity, and swelling force test

[0086] Water-holding capacity test: Weigh 0.5 g (m1) of the modified composite dietary fiber obtained in step S6 of Example 1, the composite dietary fiber obtained in step S4 of Comparative Example 1, and the modified composite dietary fiber obtained in step S6 of Comparative Example 4 into centrifuge tubes, add 15 mL of distilled water, vortex evenly, let stand at room temperature for 24 h, centrifuge at 5000 rpm for 15 min, discard the supernatant, collect the residue and weigh (m2), and calculate the water-holding capacity according to formula (1):

[0087] Water holding capacity (g / g) = (m2-m1) / m1 Equation (1)

[0088] Oil holding capacity test: Weigh 0.5 g (m1) of the modified composite dietary fiber obtained in step S6 of Example 1, the composite dietary fiber obtained in step S4 of Comparative Example 1, and the modified composite dietary fiber obtained in step S6 of Comparative Example 4 into centrifuge tubes, add 15 mL of edible oil, vortex evenly, let stand at room temperature for 24 h, centrifuge at 5000 rpm for 15 min, discard the supernatant, collect the residue and weigh (m2), and calculate the oil holding capacity according to formula (2):

[0089] Oil holding capacity (g / g) = (m2-m1) / m1 Equation (2)

[0090] Expansion force test: Weigh 0.3 g (m0) of the modified composite dietary fiber obtained in step S6 of Example 1, the composite dietary fiber obtained in step S4 of Comparative Example 1, and the modified composite dietary fiber obtained in step S6 of Comparative Example 4 into a graduated cylinder, record the volume (V1), add 10 mL of distilled water, mix well, and let stand at room temperature for 24 h, record the volume (V2), and calculate the expansion force according to formula (3):

[0091] Expansion force (mL / g) = (V2-V1) / m0 Equation (3)

[0092] The test results of Example 1, Comparative Example 1 and Comparative Example 4 are summarized in Table 1.

[0093] Table 1. Water-holding capacity, oil-holding capacity, and swelling force data

[0094]

[0095] As shown in Table 1, after removing the modification step in Comparative Example 1, the water-holding capacity, oil-holding capacity, and swelling capacity of the composite dietary fiber were significantly reduced; Comparative Example 4 used a chemical method to extract dietary fiber, which destroyed the natural binding state between dietary fiber and polyphenols, and also caused a reduction in oil-holding capacity.

[0096] Experiment 2: Determination of Free Radical Scavenging Capacity

[0097] (1) Weigh 0.5 g of the modified composite dietary fiber obtained in step S6 of Example 1 and Comparative Example 4 respectively, add 20 mL of 2 mol / L NaOH solution and hydrolyze at room temperature for 1 h with shaking, adjust the pH to 2.0 with HCl, centrifuge at 4000 rpm for 20 min and take the supernatant, extract with ethyl acetate 3 times, concentrate under vacuum at 45 °C, and redissolve the bound phenols in the obtained dietary fiber in 10 mL of 95% ethanol to obtain the sample solution, and store it in the dark for later use.

[0098] (2) Mix 4 mmol / L ABTS solution with 1.4 mmol / L potassium persulfate solution in equal volumes, and place at room temperature in the dark for 12-16 h to prepare ABTS stock solution; before use, dilute the stock solution with 95% ethanol to an absorbance of 0.7-0.8 at a wavelength of 734 nm to prepare ABTS working solution.

[0099] Group 1: 100 μL 95% ethanol solution + 400 μL ABTS working solution;

[0100] Group 2: 100 μL of sample solution obtained in step (1) + 400 μL of ABTS working solution;

[0101] After shaking the two solutions, let them stand at room temperature and in the dark for 10 minutes, and then measure the absorbance at 734 nm. Record the absorbance values ​​as A0 and A1, respectively. Calculate the ABTS free radical scavenging rate according to formula (4):

[0102] ABTS radical scavenging rate (%) = (A0-A1) / A0×100% Equation (4)

[0103] (3) Before use, dilute the 0.2 mmol / L DPPH stock solution with 95% ethanol to an absorbance of 0.7 ~ 0.8 at a wavelength of 517 nm to prepare the DPPH working solution;

[0104] Group 1: 100 μL 95% ethanol solution + 100 μL DPPH working solution;

[0105] Group 2: 100 μL of sample solution obtained in step (1) + 100 μL of DPPH working solution;

[0106] Group 3: 100 μL of the sample solution obtained in step (1) + 100 μL of 95% ethanol solution;

[0107] After shaking the above three solutions, place them at room temperature and in the dark for 30 min, and measure the absorbance at 517 nm, which are A0, A1 and A2 respectively. Calculate the DPPH free radical scavenging rate according to formula (5):

[0108] DPPH free radical scavenging rate (%) = [1-(A1-A2) / A0]×100% Equation (5)

[0109] Table 2 Free radical scavenging rate (%)

[0110]

[0111] As shown in Table 2, the composite dietary fiber extracted in Example 1 has antioxidant capacity, indicating that the extraction step can retain the bound polyphenols in the raw materials.

[0112] Experiment 3: Small Intestinal Motility Experiment

[0113] Two hundred Kunming mice, weighing 18–22 g, half male and half female, were randomly divided into a blank group, a model control group, and eight experimental groups, with 20 mice in each group. For the experimental groups, 6.66 g of the compositions prepared in Examples 1–3 and Comparative Examples 1–5 were dissolved in 40 mL of double-distilled water and stored at 4°C. The mixture was prepared daily and administered orally by gavage at a volume of 20 mL / kg bw. The blank group and the model control group received double-distilled water once daily for eight consecutive days. After this period, all groups were fasted for 16 hours. The blank group received double-distilled water, while the model control group and the experimental groups received 10 mg / kg bw of compound diphenoxylate via gavage. Thirty minutes later, the mice were administered a suspension containing 5% activated charcoal via gavage. Twenty-five minutes later, the mice were euthanized by dislocation. The mesentery was separated from the abdominal cavity, the small intestine was excised, the distance the ink was propelled was measured, and the small intestinal propulsion rate (%) was calculated.

[0114] Propulsion rate (%) = Activated carbon propulsion length (cm) / Total small intestine length (cm) × 100%.

[0115] Table 3 Small Intestinal Propulsion Rate

[0116]

[0117] As shown in Table 3, compared with the blank group, the small intestinal propulsion rate of the model control group was significantly reduced, indicating that the constipation model was successful. At the same time, the small intestinal propulsion rate of Examples 1-3 was significantly higher than that of Comparative Examples 1-5 and the model control group, indicating that the composition prepared in this invention can effectively promote gastrointestinal motility.

[0118] Application examples

[0119] The subjects met the following criteria: those with reduced bowel movement frequency and increased stool hardness; those with fewer than 3 bowel movements per week; those without organic constipation; and those with habitual constipation.

[0120] The subjects included in the criteria were randomly divided into 8 groups of 50 people each. Each group took the composition prepared in Examples 1-3 and Comparative Examples 1-5, 10g each time, twice a day, mixed with water, for 7 consecutive days. The number of bowel movements, bowel movement status and fecal characteristics of the subjects were recorded for 6 days before the trial and 7 days after the trial. The test results are shown in Table 4.

[0121] Table 4 Summary of results regarding frequency, condition, and characteristics of bowel movements

[0122]

[0123] The scoring criteria for defecation status and stool characteristics are shown in Table 5.

[0124] Table 5 Scoring Criteria

[0125]

[0126] As shown in Table 4, the frequency of bowel movements increased in groups 1-3 after the trial, and the defecation status score and stool characteristics score were improved, indicating that the composition of the present invention has a good effect on improving constipation.

[0127] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Any other modifications or equivalent substitutions made by those skilled in the art to the technical solutions of the present invention, as long as they do not depart from the spirit and scope of the technical solutions of the present invention, should be covered within the scope of the claims of the present invention.

Claims

1. A preparation process for a composition that improves gut health, characterized in that, Includes the following steps: S1. After drying rice bran, citrus peel and sea buckthorn pomace at 50℃, crush and sieve them as raw materials, add water and stir, bathe in a 95℃ water bath for 10 minutes, adjust the pH to 6.0, add α-amylase, enzymatically hydrolyze for 20 minutes, cool down to 60℃, adjust the pH to 7.5, add papain, enzymatically hydrolyze for 1 hour, inactivate the enzyme in a boiling water bath for 10 minutes, and cool to room temperature; S2. Centrifuge the solution obtained in step S1, wash the precipitate twice thoroughly with distilled water at 70-80℃, freeze-dry the precipitate under vacuum to obtain insoluble fiber powder. S3. Add 4 times the volume of 95% ethanol to the supernatant after centrifugation in step S2, let stand overnight, centrifuge, collect the precipitate, wash twice with 95% ethanol and acetone respectively, and freeze dry under vacuum to obtain soluble fiber powder. S4. Take the insoluble fiber powder obtained in step S2, add distilled water at a liquid-to-solid ratio of 1:10 (g:mL) and stir evenly, sonicate, centrifuge, collect the precipitate and freeze dry under vacuum to obtain modified insoluble dietary fiber powder. S5. Take the soluble fiber powder obtained in step S3, add distilled water at a liquid-to-solid ratio of 1:10 (g:mL) and stir evenly. Adjust the pH to 5.0 with 0.1 mol / L hydrochloric acid, add the compound enzyme, modify in a 50℃ water bath for 3 hours, then inactivate the enzyme in a boiling water bath for 10 minutes. After cooling to room temperature, add 4 times the volume of 95% ethanol and let stand overnight. Collect the precipitate, wash twice with 95% ethanol and acetone respectively, and freeze dry under vacuum to obtain modified soluble dietary fiber powder. S6. Mix the modified insoluble dietary fiber powder from step S4 and the modified soluble dietary fiber powder from step S5 to obtain modified composite dietary fiber. S7. Mix the modified composite dietary fiber, flaxseed, pea peptide, and fruit powder, pulverize, sieve, and sterilize to obtain the composition.

2. The preparation process according to claim 1, characterized in that, In step S1, the mass ratio of rice bran, citrus peel and sea buckthorn pomace is (3~5):(1~2):1, the material-to-liquid ratio is 1:15 (g:mL), the amount of α-amylase added is 0.2% of the raw material mass, and the amount of papain added is 0.6% of the raw material mass.

3. The preparation process according to claim 1, characterized in that, In step S4, the ultrasonic temperature is 45~55℃, the ultrasonic power is 300~400W, and the ultrasonic time is 30~40min.

4. The preparation process according to claim 1, characterized in that, In step S5, the compound enzyme is cellulase and xylanase in a mass ratio of 1:1, and the amount of compound enzyme added is 5% of the mass of soluble fiber powder.

5. The preparation process according to claim 1, characterized in that, In step S6, the mass ratio of modified insoluble dietary fiber powder to modified soluble dietary fiber powder is 1:(1.5~4).

6. The preparation process according to claim 1, characterized in that, In step S7, the fruit powder is one or more of the following: monk fruit powder, hawthorn powder, kiwi fruit powder, banana powder, and dragon fruit powder.

7. The composition prepared according to any one of claims 1 to 6, characterized in that, By weight, it includes the following raw material components: 50-85 parts modified compound dietary fiber, 5-8 parts flaxseed, 10-15 parts pea peptide, and 5-15 parts fruit powder.

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