A method for efficiently purifying a cyclic lipopeptide JBIR-15 from an aspergillus fermentation product

By employing a three-stage process of macroporous resin column adsorption, silica gel column chromatography, and liquid-liquid extraction, the problem of preparing gram-level high-purity JBIR-15 in existing technologies has been solved, achieving an efficient and simple purification process with a purity of 95% and a recovery rate of 45%.

CN122187900APending Publication Date: 2026-06-12ZHEJIANG UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG UNIV OF TECH
Filing Date
2026-03-16
Publication Date
2026-06-12

AI Technical Summary

Technical Problem

Existing technologies are insufficient for the efficient preparation of gram-level high-purity Aspergillus fermentation product cyclic lipopeptide JBIR-15, and liquid chromatography technology has high equipment requirements, long operation time, and low separation efficiency.

Method used

A three-stage process of macroporous resin column adsorption, silica gel column chromatography purification, and liquid-liquid extraction was adopted, combined with optimized flow rate and solvent ratio, to achieve high-efficiency purification of JBIR-15.

Benefits of technology

A JBIR-15 product with a purity of not less than 95% was obtained in a single preparation with a product recovery rate of 45%, breaking through the scale bottleneck of existing technologies and realizing gram-scale preparation.

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Abstract

The application discloses a high-efficiency purification method of cyclic lipopeptide JBIR-15 in aspergillus fermentation products, and the three-stage process of macroporous resin enrichment-silica gel column chromatography precise separation-liquid-liquid extraction deep purification is synergistically optimized, so that the technical advantages of simple operation, low equipment threshold and convenient organic solvent recovery are achieved, and the JBIR-15 product with a purity of not less than 95 % can be prepared at one time, the product recovery rate reaches 45 %, the preparation of the compound on a gram scale is successfully realized, and the scale bottleneck of the existing preparation process is effectively broken. The limitation that the existing technology depends on liquid chromatography and can only realize milligram-level preparation is solved.
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Citation Information

Patent Citations

  • Aspergillus L14-OE:: laeA2 and application thereof in production of cyclic tripeptide compounds

    CN117778204A