Pre-treatment device for decellularized matrix

Abstract

The application discloses a pretreatment device for acellular matrix, which comprises a mounting frame, a containing shell fixed to the mounting frame and a placing shell arranged in the containing shell, the containing shell is used for containing cleaning liquid, the placing shell is used for containing a substrate for preparing the acellular matrix, and the inner cavities of the two are communicated with each other. The placing shell is in transmission connection with a driving assembly, the driving assembly drives the placing shell to slowly rotate in a horizontal direction below the liquid level; meanwhile, a mixed flow assembly is linked with the driving assembly to form vertical flow in the containing shell. In use, the cleaning liquid is poured into the containing shell and covers the placing shell, the substrate is placed in the placing shell, and after the driving assembly is started, a three-dimensional vortex field formed by superposition of the circular convection and the vertical flow is obtained, the updating speed of the liquid on the surface of the substrate and the mass transfer efficiency are improved, and residue peeling and uniform cleaning are facilitated.

Description

Technical Field

[0001] This invention relates to the field of preparation of decellularized matrix, specifically to a pretreatment device for decellularized matrix. Background Technology

[0002] Decellularized matrix refers to a class of biomaterials in which cellular components of natural tissues are removed through physical, chemical, or enzymatic methods, while preserving as much of the three-dimensional structure and bioactive components of the extracellular matrix (ECM) as possible. This material retains collagen, elastin, glycosaminoglycans, and various adhesion and signaling molecules, providing cells with a microenvironment close to that of natural tissues. Therefore, it is widely used in tissue engineering, regenerative medicine, and wound repair. In its preparation, the washing step typically precedes the formal decellularization process and is mainly used to remove residual blood, loose cell debris, and soluble impurities, providing a stable and clean foundation for subsequent processing.

[0003] In conventional cleaning processes, a common method is to immerse the tissue substrate in the cleaning solution under static immersion or unidirectional agitation. While this method is simple in structure, in practical applications, the liquid tends to form a relatively stable boundary layer on the substrate surface, and local areas may experience slow flow or stagnation, causing material exchange to rely primarily on molecular diffusion. For dense or large substrates, the cleaning solution's renewal efficiency is limited, and the removal of residual substances is relatively slow. Furthermore, improving cleaning effectiveness often requires increasing agitation intensity or extending treatment time, which may increase the risk of disturbing the extracellular matrix microstructure.

[0004] Chinese invention patent CN101274106A discloses a method for preparing decellularized matrix. In this method, the tissue or organ to be used is pretreated; the tissue or organ to be used is added to a solution containing phospholipase, and a decellularized matrix is ​​prepared under controlled conditions; the prepared decellularized matrix is ​​then washed. Although this method is relatively flexible, it carries a significant risk of disturbance.

[0005] Chinese invention patent CN112961830A discloses a method for preparing and applying an acellular matrix for oral squamous cell carcinoma tissue. The method involves washing and cutting oral squamous cell carcinoma tissue into blocks; treating the blocks with 1% Trton-X100 for 12 hours on a shaker to obtain preliminary acellular tissue; treating the blocks with 1% SDS for 12 hours on a shaker; shaking the blocks with 100 U / ml DNAsel for 24 hours on a shaker; washing the blocks with PBS for 12 hours on a shaker to obtain the acellular matrix; and then sealing and storing the acellular matrix in a 0.1 U / L penicillin-streptomycin solution. While this technique is highly adaptable, it also carries the risk of disturbance.

[0006] However, there is an urgent need in the existing technology for a cleaning technology solution for decellularized matrix that can reduce disturbance to the extracellular matrix structure. Summary of the Invention

[0007] In view of the technical problems existing in the prior art, the present invention aims to provide a pretreatment device for decellularized matrix that can solve the problems of easy formation of liquid boundary layer, low mass transfer efficiency, local flow stagnation and incomplete removal of residues in the washing stage of existing decellularized matrix preparation process, and the potential disturbance to the extracellular matrix structure when the washing intensity is increased.

[0008] Specifically, the present invention solves the above-mentioned technical problems through the following technical solutions: According to one aspect of the present invention, a pretreatment device for decellularized matrix is ​​provided, comprising a mounting frame, a holding shell fixed on the mounting frame, a cleaning solution inside the holding shell, a placement shell for placing the substrate inside the holding shell, the inner cavity of the placement shell communicating with the inner cavity of the holding shell, the placement shell being drivenly connected to a drive assembly, the drive assembly being fixed on the mounting frame, and the drive assembly driving the placement shell to rotate horizontally below the liquid surface in the inner cavity of the holding shell. It also includes a mixing component, which is connected to the drive component. The drive component drives the mixing component to form a vertical vortex in the inner cavity of the container.

[0009] Sufficient cleaning liquid is poured into the container to submerge it. Then, the substrate for preparing the decellularized matrix is ​​placed inside the container. The drive assembly is activated, causing the container to rotate slowly. This causes the substrate inside the container to move within the cleaning liquid and the cleaning liquid to move on the surface of the substrate inside the container, thus creating convection in the horizontal direction. Simultaneously, the drive assembly drives the mixing assembly to operate, creating vertical liquid flow inside the container. This, combined with the horizontal convection, forms a vortex, further improving the efficiency of the cleaning liquid movement on the substrate surface, enhancing the exchange efficiency, and facilitating the cleaning of residues on the substrate surface.

[0010] In this technical solution, the housing has several evenly distributed small holes, or the surface has through grooves, and the surface of the through grooves is covered with a filter screen.

[0011] Specifically, small holes or through-slots can be made in the placement shell, and a filter screen can be covered on the through-slot. The small holes and the filter screen allow the inner cavities of the placement shell and the holding shell to be connected, so that the cleaning liquid inside the holding shell can freely enter and exit the placement shell, realizing the liquid exchange between the placement shell and the holding shell, thereby achieving the purpose of cleaning the substrate.

[0012] In this technical solution, both the holding shell and the placement shell are annular structures and are coaxially arranged. The driving component is located directly above the holding shell, and the bottom of the driving component extends into the inner ring cavity of the holding shell. The bottom of the driving component is connected to the mixing component through a transmission component.

[0013] The ring-shaped placement shell is nested inside the equally ring-shaped holding shell, which effectively utilizes space while reducing the amount of cleaning liquid required inside the holding shell.

[0014] In this technical solution, the mounting frame includes a base plate, a support plate is fixed to one side of the top of the base plate, a top plate is fixed to the top of the support plate, the container is fixed to the base plate, and the drive assembly is fixed to the top plate.

[0015] The base plate is placed on the workbench, the housing is fixed on the base plate, and the drive assembly is fixed through the top plate.

[0016] In this technical solution, the drive assembly includes a power box, which is fixed on the mounting bracket, specifically on the top plate. A drive motor is installed and fixed in the inner cavity of the power box. A drive spindle is fixed at the bottom output end of the drive motor. The bottom end of the drive spindle passes through the power box and extends into the inner cavity of the housing.

[0017] Multiple equally spaced fixing rods are fixed on the inner ring side wall of the housing. The top of the fixing rods extends upward and then bends to form an inverted "L" shape structure, and is fixed on the drive spindle.

[0018] The drive spindle coincides with the central axis of both the holding shell and the placement shell. The drive spindle on the output end of the drive motor rotates while driving the placement shell to rotate synchronously through the fixed rod, thereby realizing its rotation inside the cleaning liquid and accelerating the exchange frequency of the cleaning liquid in the inner cavity of the placement shell and the holding shell.

[0019] In this technical solution, the transmission assembly is located below the housing. The transmission assembly includes a drive bevel gear, which is fixed to the bottom end of the drive spindle. A driven bevel gear is meshed with the drive bevel gear. The driven bevel gear is fixed to the end of a transmission crossbar distributed radially along the housing. The transmission crossbar passes through the inner ring sidewall of the housing and is connected to the mixing assembly.

[0020] The transmission crossbar is fitted with a bearing bushing on the outer wall of the inner ring cavity of the container. The bearing bushing is fixed on the inner ring side wall of the container, and the transmission crossbar can rotate within the inner cavity of the bearing bushing.

[0021] The transmission crossbar is supported by a bearing bushing, and the connection between the transmission crossbar and the inner wall of the container is a rotary seal structure commonly used in the prior art.

[0022] In this technical solution, the mixing assembly includes multiple mixing sections, the number of which is the same as the number of transmission crossbars and they correspond one-to-one, and the mixing sections are located at the bottom of the housing.

[0023] The mixing shell includes a mounting plate, which is fixed vertically to the end of the corresponding transmission crossbar. Multiple mixing blades arranged in a ring array are fixed on the annular outer wall of the mounting plate.

[0024] This technical solution also includes a flow propulsion assembly, which includes multiple flow propulsion sections arranged in a ring array and located inside the housing. The flow propulsion sections are slidably connected to the outer wall of the housing via connecting sections, and the flow propulsion sections are connected to the drive assembly via a transmission section.

[0025] By creating another new liquid flow inside the housing through the propulsion component, the liquid exchange efficiency between the cleaning liquid and the substrate surface is further improved, thereby increasing the cleaning efficiency.

[0026] In this technical solution, the connecting part includes a guide slide rail with an arc-shaped structure. The guide slide rail is fitted and fixed to the side wall of the container shell. A guide slider is slidably connected to the surface of the guide slide rail. The guide slider is connected to the push part. It also includes a spring-loaded component, one end of which is fixed to the guide slider and the other end to the container shell.

[0027] In this technical solution, the flow propulsion part includes a synchronizing rod. One end of the synchronizing rod is fixed to the guide slider, and the other end of the synchronizing rod extends into the inner cavity of the placement shell and is fixed with a flow stop plate.

[0028] Several soft flow-stopping blades are fixed on the surface of the flow-stopping plate. Both the flow-stopping blades and the flow-stopping plate are arranged radially along the placement shell and vertically.

[0029] In this technical solution, the spring-loaded component includes a telescopic guide rod, and a spring is sleeved on the surface of the guide rod, with the two ends of the spring fixed to the two ends of the guide rod respectively.

[0030] In this technical solution, there is at least one transmission unit, which is fixed on the fixed rod or the extension end of the drive spindle. The transmission unit, which rotates with the drive spindle, can connect with the synchronizing rod to push the synchronizing rod to move, thereby driving the propulsion assembly to run.

[0031] Specifically, the transmission unit includes a connecting plate, which is fixed on the extension end of the fixed rod or the drive spindle. A connecting vertical shaft is fixed on the outer wall of the connecting plate. A self-rotating outer connecting sleeve is sleeved on the surface of the connecting vertical shaft. A transmission plate is fixed on the surface of the outer connecting sleeve. The transmission plate can overlap with the synchronizing rod.

[0032] A coil spring is fitted onto the surface of the connecting vertical shaft, with its two ends fixed to the connecting vertical shaft and the outer connecting sleeve, respectively.

[0033] When the coil spring does not deform, the connecting plate and the transmission plate are on the same plane and are distributed radially along the container.

[0034] According to the present invention, at least the following beneficial effects are achieved: By filling the container with sufficient cleaning fluid to completely submerge it, the substrate is treated while fully immersed, avoiding problems such as uneven drying or treatment caused by localized exposure. Furthermore, the drive assembly slowly rotates the container, creating a continuous circulatory flow of the cleaning fluid in the horizontal direction. This circulatory flow breaks down the "boundary layer" formed on the substrate surface under traditional static immersion conditions, reducing stagnant areas and thus improving the material exchange efficiency of the substrate surface.

[0035] Simultaneously, the drive component synchronously drives the mixing component, forming a stable vertical liquid flow inside the container. The vertical flow, combined with the horizontal circumferential convection, creates a three-dimensional vortex field structure within the cavity. This three-dimensional vortex can: 1. Enhances the shear resistance of the substrate surface Eddy currents generate microscale shear forces on the substrate surface, which helps to remove blood residue, loose cell debris and impurities from the ECM surface without relying on strong mechanical friction, thus reducing the risk of damage to the matrix structure.

[0036] 2. Shorten the thickness of the diffusion boundary layer In traditional static cleaning, a stable diffusion layer easily forms on the substrate surface, affecting the mass exchange between the cleaning solution and the substrate. This solution disrupts the diffusion layer through continuous flow, transforming solute exchange from simple molecular diffusion to "convection + diffusion coupled mass transfer," significantly improving the mass transfer rate.

[0037] 3. Achieve uniform cleaning across the entire surface The three-dimensional flow field avoids local dead zones or uneven flow rates, ensuring that the substrate can come into contact with the new cleaning solution in all directions, reducing the risk of residue in the central area and improving the consistency of subsequent decellularization.

[0038] 4. Improve fluid exchange efficiency Under the same cleaning fluid volume, the increased flow efficiency leads to a higher proportion of liquid exchanged per unit time, which can reduce cleaning time or fluid change frequency, thereby improving overall process efficiency.

[0039] 5. Reduce the risk of chemical damage Thanks to the improved cleaning efficiency, effective cleaning can be achieved without increasing the rotation speed or enhancing mechanical impact, thus avoiding excessive physical disturbance to the ECM fiber structure. Attached Figure Description

[0040] Figure 1This is a three-dimensional structural schematic diagram of a pretreatment device for decellularized matrix according to a specific embodiment of the present invention; Figure 2 To show Figure 1 A magnified schematic diagram of the structure at point I; Figure 3 This is a three-dimensional structural schematic diagram from another perspective of a pretreatment device for decellularized matrix according to a specific embodiment of the present invention. Figure 4 To show Figure 3 A magnified schematic diagram of the structure at point J; Figure 5 This is a bottom-view perspective view of a pretreatment device for decellularized matrix according to a specific embodiment of the present invention. Figure 6 This is a top view schematic diagram illustrating a pretreatment device for decellularized matrix according to a specific embodiment of the present invention; Figure 7 To show Figure 6 Schematic diagram of the cross-sectional structure at point AA; Figure 8 This is a schematic diagram of the three-dimensional structure of the pretreatment device for decellularized matrix according to a specific embodiment of the present invention after the shell is removed. Figure 9 To show Figure 8 A schematic diagram of the side view structure; Figure 10 To show Figure 8 A schematic diagram of the structure viewed from below.

[0041] Explanation of reference numerals in the attached figures 1. Mounting bracket; 11. Base plate; 12. Support plate; 13. Top plate; 2. Container for shells; 21. Water outlet pipe; 3. Power box; 31. Drive motor; 32. Drive spindle; 4. Transmission assembly; 41. Drive bevel gear; 42. Driven bevel gear; 43. Transmission crossbar; 44. Bearing bushing; 5. Mixing assembly; 51. Mounting plate; 52. Mixing blades; 6. Housing; 61. Fixing rod; 62. Transmission unit; 621. Connecting plate; 622. Connecting vertical shaft; 623. Outer connecting sleeve; 624. Transmission plate; 7. Flow propulsion assembly; 71. Synchronizing rod; 72. Flow stop plate; 73. Flow stop vane; 74. Guide slider; 75. Guide slide rail; 76. Guide arc rod. Detailed Implementation

[0042] The present invention will be further described below with reference to the accompanying drawings and specific embodiments. However, this description is exemplary and does not limit the present invention to the scope of the specific embodiments.

[0043] First, such as Figure 1 , 3 As shown in Figures 5 and 6, in this embodiment of the invention, the pretreatment device for decellularized matrix includes a mounting frame 1, a holding shell 2 fixed on the mounting frame 1, a cleaning solution inside the holding shell 2, and a placement shell 6 for placing the substrate inside the holding shell 2. The inner cavity of the placement shell 6 is connected to the inner cavity of the holding shell 2. The placement shell 6 is connected to a driving component, which is fixed on the mounting frame 1. The driving component drives the placement shell 6 to rotate horizontally below the liquid surface in the inner cavity of the holding shell 2. The device also includes a mixing component 5, which is connected to the driving component. The driving component drives the mixing component 5 to form a vertical vortex in the inner cavity of the holding shell.

[0044] Furthermore, sufficient cleaning liquid is poured into the holding shell 2, ensuring that the liquid level completely submerges the placement shell 6, thus guaranteeing that the substrate is always fully submerged and preventing uneven treatment caused by local exposure or alternating wet and dry conditions. After placing the substrate for preparing the decellularized matrix inside the placement shell 6, the drive assembly is activated, causing the holding shell 2 to rotate smoothly at a low speed.

[0045] During rotation, on the one hand, the substrate undergoes slow displacement along with the placement shell 6; on the other hand, the cleaning liquid forms a stable circumferential flow within the cavity, thereby creating a continuous convection field in the horizontal direction. This convection effectively disrupts the static liquid boundary layer that would otherwise easily form on the substrate surface, reduces local liquid retention, and increases the contact frequency and renewal rate between the cleaning liquid and the substrate, making it easier to remove residual blood, loose cell debris, and surface impurities.

[0046] Simultaneously, the drive component synchronously drives the mixing component 5, forming a vertical liquid circulation flow inside the container shell 2. After the vertical flow and horizontal convection are superimposed, a composite three-dimensional vortex structure is formed within the cavity. This vortex field enhances the micro-scale shearing effect of the liquid on the substrate surface, which helps to remove attached residues; on the other hand, it improves the overall mass transfer efficiency of the liquid, transforming the mass exchange from a single diffusion process to a "convection-diffusion coupled mass transfer" process, thereby significantly improving the exchange efficiency.

[0047] Further preferably, the placement shell 6 may also have several evenly distributed small holes (not shown), or a through groove (not shown) on its surface, with a filter screen (not shown) covering the surface of the through groove. The small holes and the filter screen allow the inner cavities of the placement shell 6 and the holding shell 2 to communicate, so that the cleaning liquid inside the holding shell 2 can freely enter and exit the placement shell 6, realizing the liquid exchange between the placement shell 6 and the holding shell 2, so as to achieve the purpose of cleaning the substrate.

[0048] like Figure 2 As shown, the transmission unit 62 includes a connecting plate 621, which is fixed to the fixed rod 61 or the extension end of the drive spindle 32. A connecting vertical shaft 622 is fixed to the outer wall of the connecting plate 621. A rotatable outer connecting sleeve 623 is sleeved on the surface of the connecting vertical shaft 622. A transmission plate 624 is fixed to the surface of the outer connecting sleeve 623, and the transmission plate 624 can overlap with the synchronizing rod 71. A coil spring is sleeved on the surface of the connecting vertical shaft 622, and the two ends of the coil spring are fixed to the connecting vertical shaft 622 and the outer connecting sleeve 623, respectively. When the coil spring does not deform, the connecting plate 621 and the transmission plate 624 are on the same plane and are distributed radially along the container. When the drive spindle 32 rotates, it directly drives the connecting plate 621 and the transmission plate 624 to rotate, or drives them to rotate through the fixed rod 61. After the rotating transmission plate 624 overlaps with the synchronizing rod 71, it pushes the synchronizing rod 71 and the guide slider 74 at the end of the synchronizing rod 71 to slide on the guide rail 75. This causes the stop plate 72 and the stop vane 73 at the other end of the synchronizing rod 71 to move synchronously in the cavity of the housing 6 until the guide slider 74 slides to the end of the guide rail 75, which is the limit position. During the above process, the guide arc rod 76 extends or shortens, and the spring deforms.

[0049] As the transmission rod continues to rotate, the transmission plate 624 overcomes the elastic force of the coil spring and rotates relative to the connecting plate 621 until the transmission plate 624 disengages from the synchronizing rod 71. Under the action of the spring's restoring deformation, the guiding arc rod 76 returns to its original length, thereby driving the flow stop plate 72 and the flow stop blade 73 to move back, thus forming a local flow stop.

[0050] After the transmission plate 624 disengages from the synchronizing rod 71, it returns to its original position under the action of the coil spring.

[0051] Further as Figure 1 and Figure 5 As shown, both the holding shell 2 and the placement shell 6 are annular structures and are coaxially arranged. The drive component is located directly above the holding shell 2, and the bottom of the drive component extends into the inner ring cavity of the holding shell 2. The bottom of the drive component is connected to the mixing component 5 through the transmission component 4.

[0052] In this structure, the placement shell 6 adopts a ring-shaped configuration and is nested inside the container shell 2, which also has a ring-shaped structure, so that the two form a coaxial nested arrangement. This structure significantly optimizes the space utilization efficiency inside the cavity while ensuring full immersion of the substrate and smooth fluid circulation, and has the following beneficial effects: the ring-shaped nested structure reduces the ineffective volume in the central area of ​​the container shell 2; traditional cylindrical or box-shaped cavities often have a large amount of unused internal space when placing tissue substrates, which means that more cleaning fluid must be added to achieve the requirement of complete immersion; by setting up a hollow ring-shaped placement shell 6, the substrate is distributed around the ring channel, which structurally compresses the volume of the non-working area and effectively reduces the required volume of cleaning fluid under the same external dimensions.

[0053] At least one water outlet pipe 21 with a valve is fixed on the bottom side wall of the container 2.

[0054] Mounting frame 1 includes a base plate 11, a support plate 12 is fixed to one side of the top of the base plate 11, a top plate 13 is fixed to the top of the support plate 12, the container 2 is fixed on the base plate 11, and the drive assembly is fixed on the top plate 13.

[0055] The base plate 11 is placed on the workbench, the housing 2 is fixed on the base plate 11, and the drive assembly is fixed through the top plate 13.

[0056] like Figures 2-4 As shown, the pretreatment device further includes a flow propulsion assembly 7, which comprises multiple flow propulsion sections arranged in a ring array and located inside the placement shell 6. These flow propulsion sections are slidably connected to the outer wall of the holding shell 2 via connecting sections, and are connected to the drive assembly via a transmission section 62. The flow propulsion assembly 7 creates another new liquid flow inside the placement shell 6, further improving the liquid exchange efficiency between the cleaning liquid and the substrate surface, thereby increasing the cleaning efficiency.

[0057] The connecting part includes an arc-shaped guide rail 75, which is fixed to the side wall of the container 2. A guide slider 74 is slidably connected to the surface of the guide rail 75 and is connected to the push part. It also includes a spring-loaded component, one end of which is fixed to the guide slider 74 and the other end of which is fixed to the container 2.

[0058] The flow propulsion section includes a synchronizing rod 71. One end of the synchronizing rod 71 is fixed to the guide slider 74, and the other end of the synchronizing rod 71 extends into the inner cavity of the placement shell 6 and is fixed with a flow stop plate 72. Several soft flow stop blades 73 are fixed on the surface of the flow stop plate 72. Both the flow stop blades 73 and the flow stop plate 72 are arranged radially along the placement shell 6 and vertically.

[0059] The spring-loaded component includes a telescopic guide rod 76, on the surface of which a spring is fitted, with the two ends of the spring fixed to the two ends of the guide rod 76 respectively.

[0060] There is at least one transmission part 62, which is fixed on the fixed rod 61 or the extension end of the drive spindle 32. The transmission part 62, which rotates with the drive spindle 32, can be connected with the synchronizing rod 71 to push the synchronizing rod 71 to move, thereby driving the propulsion assembly 7 to run.

[0061] Preferably, when the guide rod 76 returns to its original length, its relative sliding is damped, that is, the guide rod 76 slowly returns to its original length.

[0062] The following reference Figure 7-10 The driver components are described in detail.

[0063] like Figure 7 As shown, the drive assembly includes a power box 3, which is fixed on the mounting bracket 1, specifically on the top plate 13. A drive motor 31 is installed and fixed in the inner cavity of the power box 3. A drive spindle 32 is fixed at the bottom output end of the drive motor 31. The bottom end of the drive spindle 32 passes through the power box 3 and extends into the inner ring cavity of the housing 2. Multiple equally spaced fixing rods 61 are fixed on the inner ring side wall of the housing 6. The top of the fixing rods 61 extends upward and bends to form an inverted "L" shape structure, and is fixed on the drive spindle 32.

[0064] The drive spindle 32 coincides with the central axis of the container shell 2 and the placement shell 6. The drive spindle 32 on the output end of the drive motor 31 rotates while driving the placement shell 6 to rotate synchronously through the fixed rod 61, thereby realizing its rotation inside the cleaning liquid and accelerating the exchange frequency of the cleaning liquid in the inner cavity of the placement shell 6 and the container shell 2.

[0065] like Figure 5 , Figure 7 as well as Figure 10 As shown, the transmission assembly 4 is located below the housing 6. The transmission assembly 4 includes a drive bevel gear 41, which is fixed to the bottom end of the drive spindle 32. A driven bevel gear 42 is meshed on the drive bevel gear 41. The driven bevel gear 42 is fixed to the end of the transmission crossbar 43 distributed radially along the housing 2. The transmission crossbar 43 passes through the inner ring sidewall of the housing 2 and is connected to the mixing assembly 5.

[0066] After the drive motor 31 drives the drive spindle 32 to rotate, the drive bevel gear 41 located on the drive spindle 32 also rotates synchronously. During the synchronous rotation, the drive bevel gear 41 drives the driven bevel gear 42 that meshes with it to rotate, thereby driving the mixing component 5 on the end of the transmission crossbar 43 to run, and forming a liquid flow in the vertical direction of the inner cavity of the container 2.

[0067] A bearing sleeve 44 is fitted onto the outer wall of the inner ring cavity of the container shell 2, and the bearing sleeve 44 is fixed to the inner ring side wall of the container shell 2. The transmission crossbar 43 can rotate within the inner cavity of the bearing sleeve 44. The transmission crossbar 43 is supported by the bearing sleeve 44, and the connection between the transmission crossbar 43 and the inner wall of the container shell 2 is a rotation seal structure commonly used in the prior art.

[0068] Preferably, the diameter of the driving bevel gear 41 is larger than the diameter of the driven bevel gear 42, and the diameter of the driving bevel gear 41 is at least twice the diameter of the driven bevel gear 42. The driving bevel gear 41 rotates slowly with the drive shaft 32, and since the diameter of the driving bevel gear 41 is larger than that of the driven bevel gear 42, the mixing assembly 5 can be driven to operate faster, forming a more obvious liquid flow in the vertical direction.

[0069] like Figure 7 As shown, the mixing assembly 5 includes multiple mixing sections, the number of which is the same as the number of transmission crossbars 43 and they correspond one-to-one, and the mixing sections are located at the bottom of the housing 6; the mixing housing includes a mounting plate 51, which is fixed vertically to the end of the corresponding transmission crossbar 43, and multiple mixing blades 52 arranged in a ring array are fixed on the annular outer wall of the mounting plate 51.

[0070] The drive bevel gear 41 drives the transmission crossbar 43 to rotate via the transmission bevel gear. When the transmission crossbar 43 rotates, it drives the mounting plate 51, which is fixedly connected to its end, to rotate. During the rotation, the mixing blades 52 on its surface also rotate synchronously, and the rotating mixing blades 52 form a vertical liquid flow.

[0071] The present invention has been described in detail above. However, the present invention is not limited to the above embodiments. Those skilled in the art will understand that various changes or modifications can be made to these embodiments. Without departing from the principles and essence of the present invention, any changes in shape or structure should fall within the protection scope of the present invention. The protection scope of the present invention is defined by the appended claims.

Claims

1. A pretreatment device for decellularized matrix, comprising a mounting frame (1), wherein a holding shell (2) is fixed on the mounting frame (1), and a placement shell (6) for placing the substrate is disposed inside the holding shell (2), wherein the inner cavity of the placement shell (6) is in communication with the inner cavity of the holding shell (2), characterized in that: The placement shell (6) is connected to the drive assembly, which is fixed on the mounting frame (1). The drive assembly drives the placement shell (6) to rotate horizontally below the liquid surface in the inner cavity of the holding shell (2). It also includes a mixing component (5), which is connected to the driving component for transmission. The driving component drives the mixing component (5) to form a vertical vortex in the inner cavity of the container.

2. The pretreatment apparatus for decellularized matrix as described in claim 1, characterized in that: Both the holding shell (2) and the placement shell (6) are annular structures and are coaxially arranged. The driving component is located directly above the holding shell (2). The bottom of the driving component extends into the inner cavity of the holding shell (2), and the bottom of the driving component is connected to the mixing component (5) through the transmission component (4).

3. The pretreatment apparatus for decellularized matrix as described in claim 1, characterized in that: The mounting bracket (1) includes a base plate (11), a support plate (12) is fixed to one side of the top of the base plate (11), a top plate (13) is fixed to the top of the support plate (12), the container (2) is fixed on the base plate (11), and the drive assembly is fixed on the top plate (13).

4. The pretreatment apparatus for decellularized matrix as described in claim 2, characterized in that: The drive assembly includes a power box (3), which is fixed on the mounting bracket (1). A drive motor (31) is installed and fixed in the inner cavity of the power box (3). A drive spindle (32) is fixed at the bottom output end of the drive motor (31). The bottom end of the drive spindle (32) passes through the power box (3) and extends into the inner cavity of the housing (2). Multiple equally spaced fixing rods (61) are fixed on the inner ring sidewall of the placement shell (6). The top of the fixing rod (61) extends upward and bends to form an inverted "L" shape structure, and is fixed on the drive spindle (32).

5. The pretreatment apparatus for decellularized matrix as described in claim 4, characterized in that: The transmission assembly (4) is located below the housing (6). The transmission assembly (4) includes a drive bevel gear (41), which is fixed to the bottom end of the drive spindle (32). A driven bevel gear (42) is meshed on the drive bevel gear (41). The driven bevel gear (42) is fixed to the end of a transmission crossbar (43) that is radially distributed along the housing (2). The transmission crossbar (43) passes through the inner ring sidewall of the housing (2) and is connected to the mixing assembly (5) in a transmission manner.

6. The pretreatment apparatus for decellularized matrix as described in claim 5, characterized in that: The mixing assembly (5) includes multiple mixing sections, the number of which is the same as the number of transmission crossbars (43) and they correspond one-to-one, and the mixing sections are located at the bottom of the placement shell (6); The mixing shell includes a mounting plate (51), which is fixed vertically to the end of the corresponding transmission crossbar (43). Multiple mixing blades (52) arranged in a ring array are fixed on the annular outer wall of the mounting plate (51).

7. The pretreatment apparatus for decellularized matrix as described in claim 1, characterized in that: It also includes a flow propulsion assembly (7), which includes multiple flow propulsion sections arranged in a ring array and located inside the placement shell (6). The flow propulsion sections are slidably connected to the outer wall of the holding shell (2) through a connecting section, and the flow propulsion sections are connected to the drive assembly through a transmission section (62).

8. The pretreatment apparatus for decellularized matrix as described in claim 7, characterized in that: The connecting part includes a guide slide rail (75) with an arc-shaped structure. The guide slide rail (75) is fixed to the side wall of the container (2). A guide slider (74) is slidably connected to the surface of the guide slide rail (75). The guide slider (74) is connected to the push part. It also includes a spring-loaded component, one end of which is fixed to the guide slider (74) and the other end is fixed to the container (2).

9. The pretreatment apparatus for decellularized matrix as described in claim 8, characterized in that: The flow propulsion section includes a synchronizing rod (71), one end of which is fixed to the guide slider (74), and the other end of which extends into the inner cavity of the placement shell (6) and is fixed with a flow stop plate (72). The surface of the flow stop plate (72) is fixed with several soft flow stop blades (73). The flow stop blades (73) and the flow stop plate (72) are both arranged radially along the placement shell (6) and vertically.

10. The pretreatment apparatus for decellularized matrix as described in claim 7, characterized in that: There is at least one transmission part (62), which is fixed on the fixed rod (61) or the extension end of the drive shaft (32). The transmission part (62) that rotates with the drive shaft (32) can be connected to the synchronizing rod (71). The transmission part (62) includes a connecting plate (621), which is fixed on the extension end of the fixed rod (61) or the drive spindle (32). A connecting vertical shaft (622) is fixed on the outer wall of the connecting plate (621). A self-rotating outer connecting sleeve (623) is sleeved on the surface of the connecting vertical shaft (622). A transmission plate (624) is fixed on the surface of the outer connecting sleeve (623). The transmission plate (624) can overlap with the synchronizing rod (71). A coil spring is fitted onto the surface of the connecting vertical shaft (622).

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