An artificial breeding method for using the anoplophora malasiaca to breed anoplophora malasiaca parasitizing wasps

Abstract

This invention enables large-scale breeding of *A. glabra* wasps by using *A. glabra* as a substitute for *A. glabra*, inoculating them with *A. glabra* parasitic wasps, rearing the parasitic larvae, allowing the wasps to emerge, and then rearing the adults. This method is simple to operate, cost-effective, and highly adaptable, and can be adjusted according to specific needs. This method can achieve large-scale breeding of *A. glabra* parasitic wasps while reducing costs, ensuring continuous production throughout the year. This not only meets the needs of experimental research but also provides a large source of natural enemies to areas severely affected by *A. glabra*, thus mitigating the impact of *A. glabra* on host plants and the ecological environment.

Description

Technical Field

[0001] This invention pertains to the artificial breeding technology of natural enemy insects, specifically involving an artificial breeding method for *Anoplophora glabripennis* bred using *Anoplophora glabripennis*. Background Technology

[0002] The Asian longhorn beetle, *Anoplophora glabripennis* (Motschulsky), belongs to the family Cerambycidae in the order Coleoptera. It is a globally prevalent quarantine borer. The extent of damage caused by the Asian longhorn beetle varies in different regions, mainly due to ecological environment and host plant. It has a wide host range, affecting over 100 host plant species. In my country, it primarily damages poplar, willow, elm, and maple trees.

[0003] The most severe damage from the Asian longhorn beetle (Anoplophora glabripennis) occurs during its larval stage. This insect spends four-fifths of its life cycle boring into the trunk, with the larval stage being the most damaging. Younger larvae damage the phloem, while older larvae bore into the xylem, causing damage ranging from wind-induced trunk breakage to tree death. The insect's spread primarily relies on adults. Adult Asian longhorn beetles are strong fliers and can spread between adjacent stands, feeding on tender shoots.

[0004] Biological control is an environmentally friendly pest management strategy. Compared to chemical control, it is safer, more environmentally friendly, and provides long-lasting control. In chemical control of the Asian longhorn beetle (Spodoptera litura), the high concealment of its larvae makes it difficult to determine the optimal timing for application, leading to high costs, significant pollution, and irreversible environmental damage. Biological control, especially that utilizing natural enemy insects, is far more effective. These insects actively search for and eliminate pests and can sustainably control pest populations, exhibiting superior control efficacy. Therefore, biological control is one of the best methods for controlling the Asian longhorn beetle. Currently, only one natural enemy, the parasitic wasp, is effective against the early larval stages of the Asian longhorn beetle, and research on this early-stage enemy is relatively limited. For controlling the Asian longhorn beetle, application during the egg stage or early larval stages is a more effective option, controlling the damage to trees in its early stages.

[0005] *Spathius anoplophorae Yang*, a newly discovered dominant natural enemy of *S. anoplophorae* during its early larval stages, is currently highly dependent on the young larvae of *S. anoplophorae*. However, *S. anoplophorae* is not only expensive but also has limited production, restricting the large-scale artificial rearing of *Spathius anoplophorae Yang*. Therefore, finding a more cost-effective alternative host is crucial for reducing the breeding costs of *S. anoplophorae Yang* and achieving a large-scale, sustainable supply. This strategy is one of the key ways to ensure the large-scale, economical, and efficient reproduction of *S. anoplophorae Yang*. To address this issue, we have developed an inventive method for the artificial breeding of *S. anoplophorae Yang* using the alternative host *S. anoplophorae*. Summary of the Invention

[0006] A method for artificially breeding *Aegilops glaber*, using *Cephalotaxus fortunei*, is characterized by the following steps:

[0007] (1) Material preparation: Willow wood segments with a diameter of 2-3cm, transparent board with a diameter of 15cm×15cm×2mm, floral foam with a diameter of 22.8cm×10cm×6.5cm, artificial climate chamber, disposable sauce cups, finger tubes, medical degreased cotton rolls, hot melt glue gun and glue sticks, sewing machine, 100-mesh gauze, pruning shears, scissors, knife, Parafilm sealing film, electric arc iron, glass rod, honey, and dd water.

[0008] (2) Preparation of wood segments containing longhorn beetle: Cut healthy willow branches with a diameter of 2-4cm into 10cm long segments with pruning shears. Make two vertical cuts about 5mm wide at a distance of 1.5cm from the bottom of the wood segment. Repeat the same operation on the other side. Then lift up the phloem at the cut and remove the xylem. Place the young longhorn beetle larvae into the cut grooves. Cut the sealing film into 1cm wide strips and wrap it around the bottom, middle and end cuts of the wood segment. Each wood segment contains 8-10 larvae.

[0009] (3) Preparation of 20% honey water: Use an electric arc iron to make a hole with a diameter of 5mm in the center of the sauce cup lid, then add 2 / 3 of the water in the cup, then add 2-4 drops of honey, stir well with a glass rod, then stuff the hole with a medical absorbent cotton roll, leaving about 3mm outside the lid, and put the lid on.

[0010] (4) Insect cage: First, cut a circular hole with a diameter of 13cm in the center of two transparent boards. Then, cut a piece of gauze with a diameter of 14cm×14cm and glue it to the cut transparent board with a hot glue gun. Then, cut a piece of gauze with a diameter of 42cm×40cm and sew the two sides with a side length of 40cm together with a sewing machine. Then, glue the gauze to another transparent board with a hot glue gun. Then, glue these two transparent boards to four other complete boards to form a cube, which is the insect cage.

[0011] (5) Parasitic wasp rearing: Place the wood segment containing the insect into the insect rearing cage, add 20% honey water, and introduce parasitic wasps that have mated together. Figure 6 F). Inoculate 8-10 longhorn beetle larvae onto each log, place 5-6 logs in each rearing cage, and inoculate 18-20 female bees into each cage. Place in an artificial climate chamber at 25℃, L:D=16:8, RH=40%, and replace the laying logs approximately once a week.

[0012] Preferably, the transparent sheet is made of acrylic.

[0013] Preferably, the temperature inside the artificial climate chamber with L:D = 16:8 and RH = 40% is 25°C.

[0014] Preferably, the honey water concentration is 20%.

[0015] Beneficial effects compared to existing technologies:

[0016] Existing *Aegilops glabripennis* longhorn beetles are not only expensive but also have limited production, which restricts the large-scale artificial rearing of *Baracus stolonifer*. The rearing method provided by this invention has low rearing costs and makes population expansion easier. Attached Figure Description

[0017] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the embodiments will be described below. It should be understood that the following drawings only show some embodiments of the present invention and should not be regarded as a limitation on the scope.

[0018] Figure 1 A. The back of the insect rearing cage;

[0019] Figure 2 , B. Front view of the insect rearing cage;

[0020] Figure 3 C. Insect cage as a whole;

[0021] Figure 4 D water supply device;

[0022] Figure 5 E accessed willow wood segments containing longhorn beetle larvae;

[0023] Figure 6F is a section of willow wood that has been used to catch bees. Detailed Implementation

[0024] The purpose of this invention is to utilize an artificial breeding method for the parasitic wasp of the longhorn beetle *Anoplophora glabripennis*, ... using an alternative host, the longhorn beetle *Anoplophora glabripennis*.

[0025] A method for artificially breeding *Aegilops glaber*, using *Cephalotaxus fortunei*, is characterized by the following steps:

[0026] Specific example 1:

[0027] A method for artificially breeding *Aegilops glaber*, using *Cephalotaxus fortunei*, is characterized by the following steps:

[0028] (1) Material preparation: Willow wood segments with a diameter of 2-3cm, transparent board of 15cm×15cm×2mm (Guangdong Hongwang New Material Technology Co., Ltd.), floral foam of 22.8cm×10cm×6.5cm (Jinhua Jindong District Huaxun Liyun Packaging Co., Ltd.), artificial climate chamber (Hangzhou Lvbo Instrument Co., Ltd.), disposable sauce cups (Beijing Huiyaojia Technology Co., Ltd.), finger tubes (Huaou Glass Co., Ltd.), medical degreased cotton rolls, hot melt glue gun and glue sticks, sewing machine, 100-mesh gauze, pruning shears, scissors, knife, Parafilm sealing film, electric arc iron, glass rod, honey, and dd water.

[0029] (2) Preparation of wood segments containing longhorn beetle larvae: Cut healthy willow branches, 2-4 cm in diameter, into 10 cm long segments using pruning shears. Make two vertical upward cuts about 5 mm wide 1.5 cm from the bottom of each segment. Repeat on the other side. Then, lift up the phloem at the cuts and remove the xylem. Place the young longhorn beetle larvae into the cuts. Cut a 1 cm wide strip of sealing film and wrap it around the bottom, middle, and end cuts of each segment. Each segment should contain 8-10 larvae. Figure 5 E).

[0030] (3) Preparation of 20% honey water: Use an electric arc iron to make a 5mm diameter hole in the center of the sauce cup lid, then add 2 / 3 of the water into the cup, then add 2-4 drops of honey, stir well with a glass rod, then stuff the hole with a medical absorbent cotton roll, leaving about 3mm outside the lid, and put the lid on. Figure 4 D).

[0031] (4) Insect cage: First, cut a circular hole with a diameter of 13cm in the center of two transparent plates. Then, cut a piece of gauze measuring 14cm x 14cm and glue the gauze to the cut transparent plates using a hot glue gun. Figure 1A) Cut another piece of gauze measuring 42cm x 40cm, sew the two sides (40cm on each side) together with a sewing machine, and then use a hot glue gun to glue the gauze to another transparent board. Figure 2 B), then glue these two transparent boards to the other four complete boards to form a cube, which is the insect cage. Figure 3 C).

[0032] (5) Parasitic wasp rearing: Place the wood segment containing the insect into the insect rearing cage, add 20% honey water, and introduce parasitic wasps that have mated together. Figure 6 F). Inoculate 8-10 longhorn beetle larvae onto each log, place 5-6 logs in each rearing cage, and inoculate 18-20 female bees into each cage. Place in an artificial climate chamber at 25℃, L:D=16:8, RH=40%, and replace the laying logs approximately once a week.

[0033] Ten young larvae of the longhorn beetle were inoculated onto each willow log. Four logs were placed in each rearing cage, and 20 mated female parasitic wasps of the longhorn beetle were introduced. The cages were placed in an artificial climate chamber at 25°C, L:D = 16:8, and RH = 40%. About 22 days after inoculation, the parasitic wasps began to emerge, with approximately 100 emerging, and a female-to-male ratio of 19:1.

Claims

1. A method for artificially breeding *Begonia glabripennis*, using *Begonia glabripennis*, characterized in that... Includes the following steps: (1) Material preparation: Willow wood segments with a diameter of 2~3cm, transparent board with a diameter of 15cm×15cm×2mm, floral foam with a diameter of 22.8cm×10cm×6.5cm, artificial climate box, disposable sauce cup, finger tube, medical degreased cotton roll, hot melt glue gun and glue stick, sewing machine, 100 mesh mesh, pruning shears, scissors, knife, Parafilm sealing film, electric arc iron, glass rod, honey, dd water; (2) Preparation of wood segments containing longhorn beetle: Cut healthy willow branches with a diameter of 2-4cm into 10cm long segments with pruning shears. Make two vertical cuts about 5mm wide at a distance of 1.5cm from the bottom of the wood segment. Repeat the same operation on the other side. Then lift up the phloem at the cut and remove the xylem. Place the young longhorn beetle larvae into the cut groove. Cut the sealing film into 1cm wide strips and wrap it around the bottom, middle and end cuts of the wood segment. Each wood segment contains 8-10 larvae. (3) Preparation of 20% honey water: Use an electric arc iron to make a hole with a diameter of 5mm in the center of the sauce cup lid, then add 2 / 3 of the water in the cup, then add 2~4 drops of honey, stir well with a glass rod, then stuff the hole with a medical absorbent cotton roll, leaving about 3mm outside the lid, and put the lid on. (4) Insect cage: First, cut a circular hole with a diameter of 13cm in the center of both sides. Then cut a piece of gauze with a diameter of 14cm×14cm and glue it to the cut transparent board with a hot glue gun. Then cut a piece of gauze with a diameter of 42cm×40cm and sew the two sides with a side length of 40cm with a sewing machine. Then glue the gauze and another transparent board with a hot glue gun. Then glue the two transparent boards and the other four complete boards to form a cube, which is the insect cage. (5) Parasitic wasp rearing: Place the wood segments containing insects into the insect rearing cage, add 10%-40% honey water, introduce parasitic wasps that have mated in a concentrated manner, inoculate 8-10 longhorn beetle larvae into each wood segment, put 5-6 wood segments into each insect rearing cage, inoculate 18-20 female wasps into each cage, and place them in an artificial climate chamber with L:D=16:8 and RH=40% at 23℃-30℃. The egg-laying wood segments can be replaced about once a week.

2. The artificial breeding method for *Begonia glabripennis* using *Cephalotaxus fortunei* according to claim 1, characterized in that, The transparent panel is made of acrylic.

3. The artificial breeding method for *Begonia glabripennis* using *Cephalotaxus fortunei* according to claim 1, characterized in that, The temperature inside the artificial climate chamber with L:D=16:8 and RH=40% is 25℃.

4. The artificial breeding method for *Begonia glabripennis* using *Cephalotaxus fortunei* according to claim 1, characterized in that, The concentration of the honey water is 20%.

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