Apparatus and method for improving nk cell storage quality

Abstract

This invention discloses an apparatus and method for improving the quality of NK cell storage. It includes a storage box containing an inner box. The storage box has an operation control area and an outlet adapted to the inner box. A sealing door is provided at the outlet, rotatably connected to the storage box and sealingly covering the outlet. The front of the inner box is close to the outlet, and the rear is away from the outlet. A pull-out body is provided inside the inner box, and is slidably connected to the inner box with damping. A portion of the pull-out body is located on the front of the inner box, and the other portion is located inside the inner box, on which several storage containers are placed. A transmission mechanism is provided on the sealing door, which is connected to the outer wall of the inner box via the transmission mechanism. Driven by the sealing door, the inner box slides back and forth through the outlet and the storage box via the transmission mechanism. The beneficial effect of this invention is that it achieves the goal of not easily affecting cell activity and function.

Description

Technical Field

[0001] This invention relates to the field of cell storage technology, and in particular to an apparatus and method for improving the quality of NK cell storage. Background Technology

[0002] NK cells, or natural killer cells, are important immune cells in the body and belong to the lymphocyte category. They mainly originate from hematopoietic stem cells in the bone marrow. After maturing in the bone marrow, they enter the peripheral blood and lymphatic tissues. By culturing NK cells, we can simulate the in vivo environment in vitro to explore in depth how NK cells recognize and kill target cells, as well as the regulatory effects of various cytokines and signaling molecules on NK cell function.

[0003] Currently, NK cells typically require specific storage conditions, usually in chambers containing liquid nitrogen or cryogenic temperatures to ensure proper preservation. However, opening the chamber door often leads to a rapid exchange between the ultra-low temperature environment and room temperature air, causing rapid temperature fluctuations within the chamber. The activity and cytotoxic function of NK cells depend on the integrity of receptors on their cell membranes and the stability of intracellular cytotoxic granules. Significant temperature fluctuations can damage these delicate structures, resulting in reduced cell activity, weakened proliferative capacity, or decreased function after resuscitation, thus significantly impacting storage quality and posing a potential threat to cell viability and function. Summary of the Invention

[0004] The present invention aims to overcome the shortcomings of existing technologies in cell storage, which easily affect cell activity and function, and provides a device and method for improving the quality of NK cell storage that does not easily affect cell activity and function.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: A device for improving the quality of NK cell storage includes a storage box containing an inner box. The storage box has an operation control area and an outlet adapted to the inner box on its two adjacent outer side walls. A sealing door is provided at the outlet, rotatably connected to the storage box and sealingly covering the outlet. The front of the inner box is close to the outlet, and the rear of the inner box is away from the outlet. A pull-out body is provided inside the inner box, and the pull-out body is damped and slidably connected to the inner box. A portion of the pull-out body is located on the front of the inner box and together with the inner box forms a fully enclosed structure. The other portion of the pull-out body is located inside the inner box, and several storage containers are placed on it. A transmission mechanism is provided on the sealing door, and the sealing door is driven to the outer side wall of the inner box via the transmission mechanism. Driven by the sealing door, the inner box slides back and forth through the outlet and the storage box via the transmission mechanism.

[0006] The storage box contains an inner box. On the two adjacent outer walls of the storage box are respectively an operation control area and an outlet adapted to the inner box. A sealing door is provided at the outlet, which is rotatably connected to the storage box and seals over the outlet. The front of the inner box is close to the outlet, and the rear of the inner box is away from the outlet. A pull-out body is provided inside the inner box, which is damped and slidably connected to the inner box. A portion of the pull-out body is located on the front of the inner box and together with the inner box forms a fully enclosed structure. The other portion of the pull-out body is located inside the inner box, and several storage containers are placed on it. A transmission mechanism is provided on the sealing door, which is driven to the outer wall of the inner box via the transmission mechanism. Driven by the sealing door, the inner box slides back and forth through the outlet and into the storage box via the transmission mechanism. The regulation and control of the internal environment of the storage box used for storing NK cells is existing technology and will not be elaborated here. During cell storage, the sealed door is opened, and the inner box is simultaneously pulled out from the outlet through the transmission mechanism. This results in the inner box being partially outside and partially inside the storage box, with the outer wall of the inner box fitting against the outlet side wall. This significantly reduces the drastic exchange between the ultra-low temperature environment and room temperature air, ensuring the stability of the internal environment. Then, the NK cells are placed in the storage vessel. The pull-out mechanism is pulled out from the front of the inner box, and the storage vessel is placed inside the pull-out mechanism and pushed into the inner box. Finally, the sealed door is closed, and the inner box is inserted into the storage box. Throughout the process, the goal of minimizing the impact on cell activity and function is achieved, thereby improving storage quality. In the above process, to further improve the stability of the internal environment of the storage box, the design can be further improved so that the inner box is gradually inserted into the outlet as the sealed door begins to open, further reducing the frequency of exchange between the ultra-low temperature environment and room temperature air.

[0007] Preferably, the transmission mechanism includes two ropes symmetrically distributed about the inner box. The ropes slide parallel to the inner box. One end of each rope passes through the side wall of the storage box and is detachably and fixedly connected to the inside of the sealing door. The other end of each rope is also detachably and fixedly connected to the side wall of the inner box. A spring is located on the rear side of the inner box, and the inner box is elastically connected to the inner wall of the storage box via the spring. As the sealing door gradually opens outwards, it overcomes the elastic force of the spring and pulls the inner box outwards synchronously via the ropes. Conversely, when the sealing door is closed, the inner box gradually returns to its original position under the restoring action of the spring. This design is simple, easy to maintain, and cost-effective.

[0008] Preferably, the storage box has two through holes on its side wall, each corresponding to one of the two ropes. These two through holes are located on the left and right sides of the outlet. The outer side wall of the storage box also has two mounting grooves that communicate with the two through holes. A rotating wheel is installed in each mounting groove, embedded and rotatably connected to it. The outer circumference of the rotating wheel has an arc-shaped groove matching the rope. The portion of the rope passing through the through hole is located in one side of the arc-shaped groove on the rotating wheel. The rotatable connection between the sealing door and the storage box is located on the opposite side of the rotating wheel. The two through holes, located on the left and right sides of the outlet, prevent the rope from passing through the outlet. This facilitates the inner box's movement in close contact with the side wall of the outlet when entering or exiting the storage box, increasing the frequency of air exchange between the ultra-low temperature environment and room temperature air. It also provides good restraint for the rope, improving the accuracy of the inner box's sliding direction. During the rotation of the sealing door, the rotating wheel helps improve the smoothness of the rope's turning and protects the rope.

[0009] Preferably, the outer wall of the storage box has a groove matching the sealing door. The outlet, through hole, and mounting groove are all located within the groove. After the sealing door is embedded in the groove, it is sealed to the groove. The bottom of the sealing door is rotatably connected to the left and right sides of the groove. The top of the sealing door is equipped with a sealing lock, which locks the sealing door to the storage box. The sealing lock helps the sealing door press against the groove, thus sealing the outlet, through hole, and mounting groove. A sealing strip is usually provided on the inner side of the sealing door, which is existing technology and will not be described in detail here.

[0010] Preferably, after the sealing door is opened, its inner side is on the same horizontal plane as the bottom of the outlet, which can support the inner box. The sealing lock is located at the top of the outlet, and a handle is fixed on the outer side of the sealing door. After the sealing door is opened, the end of the handle fits against the outer wall of the storage box, which helps to enhance the supporting strength of the sealing door.

[0011] Preferably, the inner box includes an upper cover and a lower box. The upper cover and the lower box are distributed vertically and separately within the storage box. The upper cover has side plates that are vertically fixed around its perimeter and can fit against the outer circumferential wall of the lower box. The upper cover is slidably connected to the storage box and corresponds to the top of the outlet. The rear side of the upper cover is elastically connected to the inner wall of the storage box via a spring. The ropes are detachably fixed to the left and right side plates of the upper cover. The center of the front side plate of the upper cover has a clearance notch that matches the pull-out body. The pull-out body is damped and slidably connected to the lower box. A cylinder is detachably connected to the bottom of the storage box. A support platform is detachably fixed to the telescopic end of the cylinder. The support platform is slidably connected to the storage box under the drive of the cylinder. The lower box is confined to the support platform. After the lower box is closed with the upper cover under the drive of the cylinder, it corresponds to the bottom of the outlet. To protect the cylinder, it can be installed at the bottom of the storage box in a way that isolates it from the inside of the storage box. This technology is existing and will not be described in detail here. The inner box's upper cover and lower box are separated, which facilitates rapid and direct heat exchange for the NK cells stored in the storage box, allowing them to instantly reach the target temperature. This high heat exchange efficiency reduces damage to the cells caused by ice crystal formation and growth. Furthermore, in special circumstances, such as prolonged use, if the internal environment of the storage box contains other microorganisms, the upper cover and lower box can be closed for storage to avoid cross-contamination. To ensure the accuracy of the sliding position, the left and right sides of the upper cover are typically matched with the sliding rails on the left and right sides of the storage box via sliders. The upper cover is slidably connected to the storage box, and the support platform is also slidably connected to the storage box by sliding blocks on the front and rear sides, matching the sliding rails on the front and rear sides of the storage box. Both of these are existing technologies and will not be described in detail here. The pull-out body is damped and slidably connected to the lower box, which on the one hand prevents the pull-out body from shaking during the sliding of the lower box, thus ensuring the safety of the stored containers inside. On the other hand, it allows the pull-out body to return to its original position under the action of the spring. The center of the front side plate of the upper cover forms an avoidance notch that matches the pull-out body. Correspondingly, when the upper cover and the lower box are closed, the left and right sides of the front side plate can act on the left and right ends of the lower box, so that the upper cover can drive the lower box to return to its original position under the action of the spring.

[0012] Preferably, the pull-out body is L-shaped, with one side embedded in the front of the lower box and matching the clearance notch. A handle is fixed to the outside of one side of the pull-out body, and the corresponding other side of the pull-out body is located inside the lower box and has several limiting grooves that match the storage container. These limiting grooves are arranged in a rectangular array. The limiting grooves facilitate the positioning of the storage container, improve its positional stability, and ensure the quality of NK cell storage.

[0013] Preferably, the top cover matches the top of the lower box, the support platform has a rectangular hole at its center, the rectangular hole has a trapezoidal cross-section, the top diameter of the rectangular hole is smaller than its bottom diameter, a support plate with the same shape and matching shape is provided inside the rectangular hole, the top of the support plate is located inside the top of the rectangular hole and forms a limiting groove 2 that matches the bottom of the lower box between the support plate and the rectangular hole, the bottom center of the support plate is detachably fixed to the telescopic end of the cylinder, and several evenly distributed springs 2 are provided on the bottom edge of the support plate, the support plate is elastically connected to the bottom of the support platform through the springs 2. In the initial state, the lower box is confined within the limiting groove two, which helps to ensure the accurate vertical alignment of the upper cover and the lower box. When the cylinder works, it pushes the support plate upward. Since the top diameter of the rectangular hole is smaller than its bottom diameter, the support plate is locked when it reaches the same plane as the top of the support platform, thereby driving the support platform to move upward synchronously until the support platform and the bottom of the outlet are on the same horizontal plane and the upper cover and the lower box are in the closed state. Spring two helps to improve the connection strength between the support plate and the support platform, and also helps to ensure the balance of the support plate and ensure the positional stability of the storage container.

[0014] Preferably, the top of the support platform is provided with a U-shaped baffle with its opening facing the outlet. The U-shaped baffle is fixedly connected to the support platform and located at the edge of the rectangular hole. The opening end of the U-shaped baffle has an outwardly flared mouth. The flared mouth provides good guidance for the inner box to enter the U-shaped baffle, and the U-shaped baffle improves the reset accuracy of the inner box under the action of the spring.

[0015] The present invention also provides a method for improving the quality of NK cell storage, which includes the following steps: Step 1: Based on actual needs, pre-treat the inside of the storage box to ensure that the storage box has the conditions required for NK cell storage. Step two: The control system controls the cylinder to work, and the lower box moves up to close with the upper cover; Step 3: Open the sealed door. The sealed door is pulled out from the outlet by the rope, so that part of the inner box is outside the storage box and part is inside the storage box. The outer wall of the inner box is in contact with the side wall of the outlet to reduce the frequency of exchange between the ultra-low temperature environment and room temperature air inside the storage box, so as to ensure the stability of the internal environment and achieve the purpose of not easily affecting cell activity and function, thereby improving the storage quality. Step 4: Remove the extractor and place the storage container containing NK cells into the corresponding position inside the extractor. Step 5: Push the pull-out body inward to insert the storage container containing NK cells into the inner box; Step 6: Slowly close the sealing door again. Under the reset action of spring 1, the inner box will re-enter the storage box until the sealing door is completely closed. Step 7: The control system controls the cylinder to work again, and the lower box moves down and separates from the upper cover.

[0016] The beneficial effects of this invention are: 1. By reducing the frequency of exchange between the internal ultra-low temperature environment and room temperature air, the stability of the internal environment is ensured, thus achieving the goal of not easily affecting cell activity and function, thereby improving storage quality; 2. The transmission mechanism has a simple structure, is easy to maintain, and helps to save costs; 3. Two through holes matching the rope are located on the left and right sides of the outlet, so that the rope does not pass through the outlet. On the one hand, this makes it easier for the inner box to fit against the side wall of the outlet when entering and exiting the storage box, further reducing the frequency of exchange between the ultra-low temperature environment and room temperature air inside. On the other hand, it plays a good limiting role for the rope and improves the positional accuracy of the sliding direction of the inner box. 4. The inner box lid and lower box are separated, which allows NK cells stored in the storage box to quickly and directly exchange heat and reach the target temperature instantly. The heat exchange efficiency is high, thereby reducing the damage to cells caused by ice crystal formation and growth. 5. The pull-out body and the lower box are connected by a damped sliding mechanism. This prevents the pull-out body from shaking during the sliding of the lower box, thus ensuring the safety of the stored containers inside. It also allows the pull-out body to return to its original position under the action of the spring. 6. The center of the front side panel of the top cover forms an avoidance notch that matches the pull-out body. Correspondingly, when the top cover is closed with the lower box, the left and right sides of its front side panel can act on the left and right ends of the lower box, so that the top cover can drive the lower box to return to its original position under the action of spring one. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of the present invention; Figure 2 This is a structural diagram of the storage box; Figure 3 This is a schematic diagram of the cooperation structure between the inner box and the transmission mechanism; Figure 4 yes Figure 3 A schematic diagram of the structure when the upper and lower lids are closed; Figure 5 This is a top view of the present invention; Figure 6 yes Figure 5 Sectional view of EE; Figure 7 This is an exploded view of the inner box; Figure 8 yes Figure 2 Enlarged view of the structure at point A in the middle.

[0018] In the diagram: 1. Storage box, 2. Inner box, 3. Exit, 4. Sealed door, 5. Pull-out body, 6. Storage container, 7. Rope, 8. Spring 1, 9. Through hole, 10. Mounting slot, 11. Rotary wheel, 12. Arc groove, 13. Groove, 14. Sealing lock, 15. Handle, 16. Top cover, 17. Lower box, 18. Side plate, 19. Clearance notch, 20. Cylinder, 21. Support platform, 22. Handle, 23. Limiting groove 1, 24. Rectangular hole, 25. Support plate, 26. Limiting groove 2, 27. Spring 2, 28. U-shaped stop bar, 29. Trumpet mouth. Detailed Implementation

[0019] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. The following description of at least one exemplary embodiment is merely illustrative and is in no way intended to limit this application or its application or use. All other embodiments obtained by those skilled in the art based on the embodiments of this application without creative effort are within the scope of protection of this application.

[0020] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0021] Unless otherwise specifically stated, the relative arrangement, numerical expressions, and values ​​of components illustrated in these embodiments do not limit the scope of this application. For ease of illustration, spatial relative terms such as “upper,” “lower,” “left,” and “right” are used in the embodiments to describe the relationship of one element or feature shown in the figures relative to another element or feature. It should be understood that, in addition to the orientations shown in the figures, spatial terms are intended to include different orientations of the device in use or operation. For example, if the device in the figures is inverted, an element described as being “below” other elements or features would be fixed “upper” to other elements or features. Thus, the exemplary term “lower” can include both upper and lower orientations. The device may be fixed in other ways (rotated 90 degrees or located in other orientations), and the spatial relative descriptions used herein can be interpreted accordingly. It should also be understood that, for ease of description, the dimensions of the various parts shown in the figures are not drawn to actual scale. Techniques, processes, and equipment known to those skilled in the art may not be discussed in detail, but where appropriate, such techniques, processes, and equipment should be considered part of the specification. In all examples shown and discussed herein, any specific values ​​should be interpreted as merely exemplary and not as limiting. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters in the following figures denote similar items; therefore, once an item is defined in one figure, it need not be discussed further in subsequent figures.

[0022] Furthermore, it should be noted that the use of terms such as "first" and "second" to define components is merely for the purpose of distinguishing the corresponding components. Unless otherwise stated, the above terms have no special meaning and therefore cannot be construed as limiting the scope of protection of this application.

[0023] like Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 5In the embodiments described, an apparatus for improving the quality of NK cell storage includes a storage box 1, an inner box 2 inside the storage box 1, an operation control area and an outlet 3 adapted to the inner box 2 on the two adjacent outer side walls of the storage box 1, a sealing door 4 at the outlet 3, the sealing door 4 being rotatably connected to the storage box 1 and sealingly covering the outlet 3, the front side of the inner box 2 being close to the outlet 3, and the rear side of the inner box 2 being away from the outlet 3, a pull-out body 5 inside the inner box 2, the pull-out body 5 being damped and slidably connected to the inner box 2, a part of the pull-out body 5 being located on the front side of the inner box 2 and forming a fully enclosed structure together with the inner box 2, the other part of the pull-out body 5 being located inside the inner box 2 and having several storage containers 6 placed on it, a transmission mechanism being provided on the sealing door 4, the sealing door 4 being driven and connected to the outer side wall of the inner box 2 through the transmission mechanism, the inner box 2 being driven by the sealing door 4 and slidably connected to the storage box 1 through the outlet 3 through the transmission mechanism.

[0024] like Figure 3 , Figure 4 , Figure 6 and Figure 7 As shown, the transmission mechanism includes two ropes 7, which are symmetrically distributed about the inner box 2. The sliding directions of the ropes 7 and the inner box 2 are parallel to each other. One end of the rope 7 passes through the side wall of the storage box 1 and is detachably and fixedly connected to the inside of the sealing door 4. The other end of the rope 7 is detachably and fixedly connected to the side wall of the inner box 2. A spring 8 is provided on the rear side of the inner box 2, and the inner box 2 is elastically connected to the inner side wall of the storage box 1 through the spring 8.

[0025] like Figure 2 and Figure 8 As shown, the storage box 1 has two through holes 9 on its side wall, each matching one of the two ropes 7. The two through holes 9 are located on the left and right sides of the outlet 3, respectively. The outer side wall of the storage box 1 has two mounting grooves 10 that are connected to the two through holes 9, respectively. A rotating wheel 11 is installed in the mounting groove 10. The rotating wheel 11 is embedded in the mounting groove 10 and is rotatably connected to the mounting groove 10. The outer circumference of the rotating wheel 11 has an arc-shaped groove 12 that matches the rope 7. The part of the rope 7 that passes through the through hole 9 is located in the arc-shaped groove 12 on one side of the rotating wheel 11. The rotatable connection between the sealing door 4 and the storage box 1 is located on the other side corresponding to the rotating wheel 11.

[0026] like Figure 2 and Figure 6 As shown, the outer wall of the storage box 1 is provided with a groove 13 that matches the sealing door 4. The outlet 3, through hole 9 and mounting groove 10 are all located in the groove 13. After the sealing door 4 is embedded in the groove 13, it is sealed and connected to the groove 13. The bottom of the sealing door 4 is rotatably connected to the left and right sides of the groove 13. The top of the sealing door 4 is provided with a sealing lock 14. The sealing door 4 is locked to the storage box 1 by the sealing lock 14.

[0027] like Figure 1 As shown, after the sealing door 4 is opened, its inner side is on the same horizontal plane as the bottom of the outlet 3. The sealing lock 14 is located at the top of the outlet 3. A handle 15 is fixed on the outer side of the sealing door 4. After the sealing door 4 is opened, the end of the handle 15 fits against the outer wall of the storage box 1.

[0028] like Figure 3 , Figure 4 , Figure 6 and Figure 7 As shown, the inner box 2 includes an upper cover 16 and a lower box 17. The upper cover 16 and the lower box 17 are distributed inside the storage box 1 in a vertically corresponding manner and are separate from each other. The upper cover 16 has side plates 18 that are vertically fixed around its perimeter and can fit against the outer side wall of the lower box 17. The upper cover 16 is slidably connected to the storage box 1 and corresponds to the top of the outlet 3. The rear side of the upper cover 16 is elastically connected to the inner side wall of the storage box 1 by a spring 8. The ropes 7 are detachably and fixedly connected to the left and right side plates 18 of the upper cover 16. The front side plate 18 of the upper cover 16 has a clearance notch 19 that matches the pull-out body 5. The pull-out body 5 is damped and slidably connected to the lower box 17. A cylinder 20 is detachably connected to the bottom of the storage box 1. A support platform 21 is detachably fixed to the telescopic end of the cylinder 20. The support platform 21 is slidably connected to the storage box 1 under the drive of the cylinder 20. The lower box 17 is limited to the support platform 21. After the lower box 17 is closed with the upper cover 16 under the drive of the cylinder 20, it corresponds to and matches the bottom of the outlet 3.

[0029] The pull-out body 5 is L-shaped. One side of the pull-out body 5 is embedded in the front side of the lower box 17 and matches the clearance notch 19. A handle 22 is fixed on the outside of one side of the pull-out body 5. The other side of the pull-out body 5 is located inside the lower box 17 and is provided with several limiting grooves 23 that match the storage container 6. The several limiting grooves 23 are distributed in a rectangular array.

[0030] The top cover 16 matches the top of the lower box 17. The center of the support platform 21 is provided with a rectangular hole 24. The cross-sectional shape of the rectangular hole 24 is trapezoidal. The top diameter of the rectangular hole 24 is smaller than its bottom diameter. A support plate 25 with the same shape and matching shape is provided inside the rectangular hole 24. The top of the support plate 25 is located inside the top of the rectangular hole 24 and forms a limiting groove 26 that matches the bottom of the lower box 17 between the support plate 25 and the rectangular hole 24. The bottom center of the support plate 25 is detachably and fixedly connected to the telescopic end of the cylinder 20. Several evenly distributed springs 27 are provided on the bottom edge of the support plate 25. The support plate 25 is elastically connected to the bottom of the support platform 21 through the springs 27.

[0031] The top of the support platform 21 is provided with a U-shaped baffle 28 with an opening facing the outlet 3. The U-shaped baffle 28 is fixedly connected to the support platform 21 and is located at the edge of the rectangular hole 24. The opening end of the U-shaped baffle 28 is provided with an outwardly flared mouth 29.

[0032] The present invention also provides a method for improving the quality of NK cell storage, which includes the following steps: Step 1: Based on actual needs, pre-treat the interior of storage box 1 to ensure that storage box 1 has the conditions required for NK cell storage. Step 2: The control system controls the cylinder 20 to work, and the lower box 17 moves up to close with the upper cover 16; Step 3: Open the sealing door 4. The sealing door 4 is pulled synchronously by the rope 7 to pull the entire inner box 2 out of the outlet 3, so that part of the inner box 2 is outside the storage box 1 and part is inside the storage box 1. The outer wall of the inner box 2 is in contact with the side wall of the outlet 3; this reduces the frequency of exchange between the ultra-low temperature environment and the room temperature air inside, and ensures the stability of the internal environment. Step 4: Remove the pull-out body 5 and place the storage container 6 containing NK cells into the corresponding position inside the pull-out body 5. Step 5: Push the pull-out body 5 inward to insert the storage container 6 containing NK cells into the inner box 2; Step 6: Slowly close the sealing door 4 again. Under the reset action of spring 8, the inner box 2 re-enters the storage box 1 until the sealing door 4 is completely closed. Step 7: The control system controls cylinder 20 to work again, and the lower box 17 moves down and separates from the upper cover 16. This allows the NK cells stored in the storage box 1 to quickly and directly exchange heat, instantly reaching the target temperature. The heat exchange efficiency is high, thereby reducing the damage to cells caused by ice crystal formation and growth.

[0033] In step two above, in the initial state, the lower box 17 is confined within the limiting groove 26, which helps to ensure the accuracy of the vertical alignment between the upper cover 16 and the lower box 17. When the cylinder 20 works, it pushes the support plate 25 upward. Since the top diameter of the rectangular hole 24 is smaller than its bottom diameter, the support plate 25 is locked when it moves upward to the same plane as the top of the support platform 21, thereby driving the support platform 21 to move upward synchronously until the support platform 21 and the bottom of the outlet 3 are on the same horizontal plane and the upper cover 16 and the lower box 17 are in the closed state. The spring 27 helps to improve the connection strength between the support plate 25 and the support platform 21, and also helps to ensure the balance of the support plate 25 and the positional stability of the storage container 6.

[0034] Through the above process, the frequency of exchange between the ultra-low temperature environment and room temperature air inside the storage box 1 is effectively reduced, ensuring the stability of the internal environment and achieving the goal of not easily affecting cell activity and function, thereby improving storage quality. In the above process, firstly, in order to improve the stability of the internal environment of the storage box 1, the design can be further improved by adjusting the size so that the inner box 2 gradually inserts into the outlet 3 as the sealing door 4 begins to open, in order to further reduce the frequency of exchange between the ultra-low temperature environment and room temperature air inside the storage box 1. Secondly, in order to ensure the accuracy of the sliding position, the left and right sides of the top cover 16 are usually matched with the slide rails on the left and right sides of the storage box 1 and slid back and forth inside the storage box 1 through sliders, and the support platform 21 is also matched with the slide rails on the front and back sides of the storage box 1 and slid up and down inside the storage box 1 through sliders on the front and back sides. Both of these are existing technologies and will not be described in detail here.

[0035] In the above process, the regulation and control of the internal environment of the storage box 1 used for NK cell storage is prior art, so it will not be described in detail here; in order to protect the cylinder 20, the cylinder 20 can be installed at the bottom of the storage box 1 in a way that isolates it from the inside of the storage box 1. This technology is prior art, so it will not be described in detail here.

[0036] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit them. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A device for improving the quality of NK cell storage, characterized in that, The system includes a storage box (1), an inner box (2) inside the storage box (1), an operation control area and an outlet (3) adapted to the inner box (2) on the two adjacent outer side walls of the storage box (1), a sealing door (4) at the outlet (3), the sealing door (4) being rotatably connected to the storage box (1) and sealingly covering the outlet (3), the front side of the inner box (2) being close to the outlet (3), the rear side of the inner box (2) being far from the outlet (3), and a pull-out body (5) inside the inner box (2). The inner box (2) is damped and slidably connected. A part of the pull-out body (5) is located on the front side of the inner box (2) and together with the inner box (2) forms a fully enclosed structure. The other part of the pull-out body (5) is located inside the inner box (2) and several storage containers (6) are placed on it. The sealing door (4) is provided with a transmission mechanism. The sealing door (4) is connected to the outer wall of the inner box (2) through the transmission mechanism. Under the drive of the sealing door (4), the inner box (2) is slidably connected to the storage box (1) through the transmission through the outlet (3) of the transmission mechanism.

2. The device for improving the quality of NK cell storage according to claim 1, characterized in that, The transmission mechanism includes two ropes (7), which are symmetrically distributed on the left and right sides with the inner box (2) as the center. The sliding directions of the ropes (7) and the inner box (2) are parallel to each other. One end of the rope (7) passes through the side wall of the storage box (1) and is detachably and fixedly connected to the inside of the sealing door (4). The other end of the rope (7) is detachably and fixedly connected to the side wall of the inner box (2). A spring (8) is provided on the rear side of the inner box (2). The inner box (2) is elastically connected to the inner side wall of the storage box (1) through the spring (8).

3. The device for improving the quality of NK cell storage according to claim 2, characterized in that, The storage box (1) has two through holes (9) on its side wall that are respectively matched with two ropes (7). The two through holes (9) are located on the left and right sides of the outlet (3). The storage box (1) has two mounting grooves (10) on its outer side wall that are respectively connected to the two through holes (9). The mounting groove (10) has a rotating wheel (11) inside it. The rotating wheel (11) is embedded in the mounting groove (10) and is rotatably connected to the mounting groove (10). The outer circumference of the rotating wheel (11) has an arc groove (12) that matches the rope (7). The part of the rope (7) that passes through the through hole (9) is located in the arc groove (12) on one side of the rotating wheel (11). The rotating connection between the sealing door (4) and the storage box (1) is located on the other side of the rotating wheel (11).

4. The device for improving the quality of NK cell storage according to claim 3, characterized in that, The outer wall of the storage box (1) is provided with a groove (13) that matches the sealing door (4). The outlet (3), through hole (9) and mounting groove (10) are all located in the groove (13). After the sealing door (4) is embedded in the groove (13), it is sealed and connected to the groove (13). The bottom of the sealing door (4) is rotatably connected to the left and right sides of the groove (13). The top of the sealing door (4) is provided with a sealing lock (14). The sealing door (4) is locked to the storage box (1) by the sealing lock (14).

5. The device for improving the quality of NK cell storage according to claim 4, characterized in that, After the sealing door (4) is opened, its inner side is on the same horizontal plane as the bottom of the outlet (3). The sealing lock (14) is located at the top of the outlet (3). A handle (15) is fixed on the outer side of the sealing door (4). After the sealing door (4) is opened, it fits against the outer wall of the storage box (1) through the end of the handle (15).

6. An apparatus for improving the quality of NK cell storage according to claim 2, 3, 4, or 5, characterized in that, The inner box (2) includes an upper cover (16) and a lower box (17). The upper cover (16) and the lower box (17) are distributed in the storage box (1) in a vertically corresponding manner and are separated from each other. The upper cover (16) has side plates (18) that are vertically fixed around its perimeter and can fit against the outer side wall of the lower box (17). The upper cover (16) is slidably connected to the storage box (1) and corresponds to the top of the outlet (3). The rear side of the upper cover (16) is elastically connected to the inner side wall of the storage box (1) by a spring (8). The ropes (7) are detachably and fixedly connected to the left and right side plates (18) of the upper cover (16). The front side plate (18) of the top cover (16) has a clearance notch (19) that matches the pull-out body (5) in the center. The pull-out body (5) is damped and slidably connected to the lower box (17). The bottom of the storage box (1) is detachably connected to a cylinder (20). A support platform (21) is detachably fixed on the telescopic end of the cylinder (20). The support platform (21) is slidably connected to the storage box (1) under the drive of the cylinder (20). The lower box (17) is limited to the support platform (21). After the lower box (17) is closed with the top cover (16) under the drive of the cylinder (20), it corresponds to and matches the bottom of the outlet (3).

7. The device for improving the quality of NK cell storage according to claim 6, characterized in that, The pull-out body (5) is L-shaped. One side of the pull-out body (5) is embedded in the front side of the lower box (17) and matches the clearance notch (19). A handle (22) is fixed on the outside of one side of the pull-out body (5). The other side of the pull-out body (5) is located inside the lower box (17) and is provided with several limiting grooves (23) that match the storage container (6). The several limiting grooves (23) are distributed in a rectangular array.

8. The device for improving the quality of NK cell storage according to claim 6, characterized in that, The top cover (16) matches the top of the lower box (17). The center of the support platform (21) is provided with a rectangular hole (24). The cross-sectional shape of the rectangular hole (24) is trapezoidal. The top diameter of the rectangular hole (24) is smaller than its bottom diameter. The rectangular hole (24) is provided with a support plate (25) that is compatible with it and has the same shape. The top of the support plate (25) is located inside the top of the rectangular hole (24) and forms a limiting groove (26) that matches the bottom of the lower box (17) between the support plate (25) and the rectangular hole (24). The bottom center of the support plate (25) is detachably and fixedly connected to the telescopic end of the cylinder (20). The bottom edge of the support plate (25) is provided with several evenly distributed springs (27). The support plate (25) is elastically connected to the bottom of the support platform (21) through the springs (27).

9. The device for improving the quality of NK cell storage according to claim 8, characterized in that, The top of the support platform (21) is provided with a U-shaped baffle (28) with an opening facing the outlet (3). The U-shaped baffle (28) is fixedly connected to the support platform (21) and located at the edge of the rectangular hole (24). The opening end of the U-shaped baffle (28) is provided with an outwardly flared mouth (29).

10. A method for improving the quality of NK cell storage according to claim 6, characterized in that, Includes the following steps: Step 1: Based on actual needs, pre-treat the inside of the storage box (1) to ensure that the storage box (1) has the conditions required for NK cell storage. Step 2: The control system controls the cylinder (20) to work, and the lower box (17) moves up to close the upper cover (16); Step 3: Open the sealing door (4). The sealing door (4) is pulled synchronously by the rope (7) to pull the entire inner box (2) out of the outlet (3), so that part of the inner box (2) is outside the storage box (1) and part is inside the storage box (1), and the outer wall of the inner box (2) is in contact with the side wall of the outlet (3). Step 4: Remove the pull-out body (5) and place the storage container (6) containing NK cells into the corresponding position inside the pull-out body (5); Step 5: Push the pull-out body (5) inward to insert the storage container (6) containing NK cells into the inner box (2); Step 6: Slowly close the sealing door (4) again. Under the reset action of spring 1 (8), the inner box (2) re-enters the storage box (1) until the sealing door (4) is completely closed. Step 7: The control system controls the cylinder (20) to work again, and the lower box (17) moves down and separates from the upper cover (16).

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