TaFIPR-D gene and biomaterials in improving plant resistance to fungal diseases

Abstract

This application discloses the application of the TaFIPR-D gene or biological materials containing the TaFIPR-D gene in enhancing plant resistance to fungal diseases. By increasing the content level of the receptor-like kinase protein TaFIPR in plant cells, this application enhances the recognition of the polypeptide TaFIP, which is beneficial for expanding disease-resistant immune signals, thereby effectively improving plant resistance to Fusarium graminearum and / or Fusarium pseudograminearum.

Description

Technical Field

[0001] This application relates to the field of plant genetic engineering technology, and in particular to the application of the TaFIPR-D gene and its biomaterials in enhancing plant resistance to fungal diseases. Background Technology

[0002] As a core food crop widely cultivated globally, the safe production of wheat is directly related to food security and people's well-being. In recent years, affected by factors such as changes in farming patterns and fluctuating climate conditions, the occurrence of wheat scab and stem rot caused by Fusarium graminearum and Fusarium pseudograminearum, respectively, has become increasingly serious, spreading widely in major wheat-producing areas in my country and the world. This has not only led to a sharp decline in wheat yield and quality, but also posed a serious threat to human and animal health due to the fungal toxins such as deoxynivalenol (DON) and zearalenone (ZEN) produced by the pathogens during infection and wheat storage.

[0003] Currently, due to the lack of major disease-resistant genes in wheat and the low level of wheat resistance, the main control measures are: on the basis of breeding disease-resistant varieties, combined with chemical control measures. However, long-term and large-scale application of chemical agents can easily induce drug resistance in pathogens and cause environmental pollution. Summary of the Invention

[0004] In view of the problems of the prior art, this application provides the application of the TaFIPR-D gene and its biomaterials in improving plant resistance to fungal diseases.

[0005] This application provides the application of the TaFIPR-D gene in enhancing plant resistance to fungal diseases, wherein the nucleotide sequence of the TaFIPR-D gene is shown in SEQ ID NO: 1.

[0006] The TaFIPR-D gene encodes a receptor-like kinase protein, TaFIPR, which can recognize the peptide TaFIP. The amino acid sequence of the receptor-like kinase protein TaFIPR is shown in SEQ ID NO: 2.

[0007] Among them, the peptide TaFIP belongs to the wheat endogenous TaFIP peptide family. These wheat endogenous peptides are mainly peptides with fewer than 80 amino acids and contain a signal peptide but no transmembrane domain. The peptide TaFIP shown in SEQ ID NO: 3 was screened from these, and it can activate wheat MAPK and other immune defense responses.

[0008] MAPK refers to the process by which mitogen-activated protein kinase, upon signal stimulation, changes from an inactive state to an active state through phosphorylation modification, thereby initiating downstream signaling cascade reactions.

[0009] This application also provides biomaterials containing the TaFIPR-D gene and their application in enhancing plant resistance to fungal diseases.

[0010] Specifically, the biological material can be selected from expression cassettes, recombinant vectors, or recombinant microorganisms.

[0011] The expression cassette is an artificially constructed functional DNA unit that typically includes a promoter, the target gene coding sequence, and a terminator. It contains the essential regulatory elements required for the target gene to function properly in the host cell.

[0012] Optionally, the expression cassette includes a UBI promoter, TaFIPR-D, a green fluorescent protein (GFP) tracer tag, and a NOS terminator.

[0013] Recombinant vectors are vectors into which expression cassettes are inserted (such as plasmids or viral vectors), serving as transport vehicles for the target gene. Optionally, the vector molecule can be a dual-source overexpression vector.

[0014] Recombinant microorganisms are microorganisms into which a recombinant vector containing a target gene is introduced through genetic engineering. They can serve as mediators for plant transgenic processes; for example, the recombinant vector can be transferred into engineered Agrobacterium, which can then be used to transfect plant tissues, thereby integrating the target gene into the wheat genome. Optionally, the engineered Agrobacterium is Agrobacterium rhizogenes (…). Agrobacterium tumefaciens GV3101.

[0015] This application improves the plant's resistance to fungal diseases by overexpressing the TaFIPR-D gene in plant cells, thereby upregulating the level of the receptor-like kinase protein TaFIPR, enhancing the recognition of the endogenous polypeptide TaFIP, expanding the disease-resistant immune signal, and thus effectively improving the plant's resistance to fungal diseases.

[0016] Specifically, the application includes: introducing the TaFIPR-D gene into the genome of a recipient plant to obtain a transgenic plant; the transgenic plant is capable of overexpressing the TaFIPR-D gene.

[0017] Optionally, the application further includes: applying the peptide TaFIP to the transgenic plant. The peptide TaFIP can induce stronger MAPK activity in the transgenic plant.

[0018] Optionally, the amino acid sequence of the polypeptide TaFIP is shown in SEQ ID NO: 3.

[0019] Optionally, the polypeptide TaFIP is artificially synthesized.

[0020] Optionally, a solution of the polypeptide TaFIP can be prepared in advance using a solvent before being applied to the transgenic plant.

[0021] Optionally, the solvent is water.

[0022] Optionally, the concentration of the solution is 1 nM to 1 μM; preferably 100 nM.

[0023] Optionally, the recipient plant is wheat or Arabidopsis thaliana.

[0024] Optionally, the fungal disease resistance includes resistance to Fusarium graminearum and / or Fusarium pseudograminearum infection.

[0025] This application also provides a method for improving wheat's resistance to fungal diseases, including: The TaFIPR-D gene was introduced into the genome of recipient wheat via an expression vector and engineered Agrobacterium to obtain transgenic wheat; the nucleotide sequence of the TaFIPR-D gene is shown in SEQ ID NO: 1.

[0026] Transgenic wheat obtained through genetic engineering exhibits significant resistance to Fusarium graminearum and / or Fusarium pseudograminearum, preventing wheat scab and stem rot without affecting wheat growth.

[0027] Optionally, the genetically modified wheat is a homozygous line.

[0028] Optionally, the method further includes: applying the polypeptide TaFIP to the transgenic wheat, as detailed above, and will not be repeated here.

[0029] This application also provides a method for breeding resistant wheat, comprising: The TaFIPR-D gene was introduced into the genome of recipient wheat via an expression vector and engineered Agrobacterium to obtain transgenic wheat; the nucleotide sequence of the TaFIPR-D gene is shown in SEQ ID NO: 1.

[0030] Compared with existing technologies, this application enhances the recognition of the TaFIP polypeptide family by increasing the expression level of the receptor-like kinase protein TaFIPR in wheat cells, thereby expanding the disease-resistant immune signal and effectively improving the resistance of wheat to Fusarium graminearum and / or Fusarium pseudograminearum. Attached Figure Description

[0031] Figure 1A This is a schematic diagram of the expression box in this application; Figure 1B This is a schematic diagram of the recombinant vector of this application; Figure 2This is a gel electrophoresis image of the TaFIPR-D gene in the transgenic wheat of this application; Figure 3 This is a graph showing the expression levels of the TaFIPR-D gene in various wheat lines in this application. Figure 4 The polypeptide TaFIP in this application is used in the homozygous transgenic line TaFIPR-D OE The result of MAPK activation induced by the medium is shown in the figure; Figure 5 This is a diagram showing the effect of the polypeptide TaFIP in this application on the expression of immune defense genes in transgenic wheat lines; Figure 6A Phenotypic diagrams of the resistance of various wheat lines to Fusarium graminearum infection in this application; Figure 6B This is a statistical chart showing the resistance of various wheat lines to Fusarium graminearum infection in this application; Figure 7A Phenotypic diagrams of the resistance of various wheat lines to Fusarium simulans infection in this application; Figure 7B This is a statistical chart showing the resistance of various wheat lines to Fusarium graminearum infection in this application. Detailed Implementation

[0032] The technical solutions described in this application will be further explained below with reference to specific embodiments, but this application is not limited thereto.

[0033] The specific formulation of the culture medium involved in this application is as follows: LB solid medium: 10 g / L tryptone, 5 g / L yeast extract, 10 g / L sodium chloride, 15 g / L agar powder, 50 μg / mL kanamycin sulfate, 20 μg / mL rifampin.

[0034] LB liquid medium: 10 g / L tryptone, 5 g / L yeast extract, 10 g / L sodium chloride, 50 μg / mL kanamycin sulfate, 20 μg / mL rifampin.

[0035] MG / L liquid culture medium: 5 g / L peptone, 2.5 g / L yeast extract, 100 mg / L sodium chloride, 5 g / L mannitol, 1 g / L glutamate, 250 mg / L KH2PO4, 100 mg / L MgSO4, 1 μg / L biotin, 50 μg / mL kanamycin sulfate, 20 μg / mL rifampin.

[0036] Liquid infection medium: 0.44 g / L MS, 10 g / L glucose, 0.5 g / L MES, 150 μM acetylsylgenone.

[0037] Co-culture medium: 0.44 g / L MS, 10 g / L glucose, 0.5 g / L MES, 150 μM acetylsalicylic acid, 5 μM silver nitrate, 1.25 mg / L copper sulfate, 8 g / L agar powder.

[0038] Screening medium: 4.3 g / L MS, 30 g / L maltose, 1 g / L hydrolyzed casein, 1 mg / L zeatin, 1.25 mg / L copper sulfate, 2 mg / L Picloram, 0.5 mg / L 2,4-dichlorophenoxyacetic acid (2,4-D), 5 g / L agar powder, 160 mg / L termethin, 10 mg / L glufosinate.

[0039] Regeneration medium: 4.3 g / L MS, 30 g / L maltose, 1 g / L hydrolyzed casein, 1 mg / L zeatin, 1.25 mg / L copper sulfate, 3.5 g / L plant gel, 160 mg / L termethin, 5 mg / L glufosinate.

[0040] Rooting medium: 2.2 g / L MS, 3.5 g / L plant gel, 160 mg / L termethin, 5 mg / L glufosinate.

[0041] Example 1 1.1 Obtaining the TaFIPR-D gene and its encoded protein Transcriptome data of wheat before and after infection with Fusarium graminearum (the causal agent of wheat blight) were obtained from the public database WheatOmics to identify endogenous wheat peptides induced by Fusarium graminearum. These endogenous wheat peptides were mainly peptides with fewer than 80 amino acids and containing a signal peptide but lacking a transmembrane domain. The peptide TaFIP, as shown in SEQ ID NO: 3, was screened and can activate wheat's MAPK immune defense response.

[0042] Using an Arabidopsis heterologous expression system, the receptor-like kinase protein TaFIPR was screened and identified as being able to recognize the wheat endogenous peptide TaFIP and activate the immune response, thereby regulating resistance to Fusarium.

[0043] Based on the protein sequence information of the receptor-like kinase protein TaFIPR (as shown in SEQ ID NO: 2), the coding region sequence of its corresponding gene was retrieved from the database, as shown in SEQ ID NO: 1.

[0044] 1.2 Obtaining homozygous transgenic wheat lines and seeds (1) Construction of recombinant carrier The expression cassette of the TaFIPR-D gene was artificially synthesized (Figure 1A), and the expression cassette was inserted into the dual-source expression vector pSV1 to construct the recombinant vector TaFIPR-OE (Figure 1B).

[0045] (2) Preparation of recombinant Agrobacterium The recombinant vector TaFIPR-OE was transformed into Agrobacterium rhizogenes using the freeze-thaw method. Agrobacterium tumefaciens GV3101 competent cells; the transformed bacterial culture was spread on LB solid medium and cultured at 28°C for 2 days.

[0046] Positive single clones were picked and inoculated into LB liquid medium containing the same antibiotic, and cultured at 28°C with shaking at 180 rpm until the bacterial OD reached the target value. 600 The concentration was 0.6, and recombinant Agrobacterium carrying the TaFIPR-OE plasmid was obtained for subsequent wheat transformation.

[0047] (3) Genetic transformation and co-culture of wheat embryos Select wheat embryos that are 12-15 days after pollination and 1.5-2.0 mm in length. After peeling and disinfecting, place the embryos with the scutellum facing upwards.

[0048] Recombinant Agrobacterium was inoculated into MG / L liquid medium and cultured at 28℃ with shaking at 180 rpm until OD reached. 600 The concentration was 0.6, and after centrifugation, the sample was resuspended in liquid infection medium and 150 μM acetylsuccinone was added to obtain a recombinant Agrobacterium suspension.

[0049] Wheat embryos were immersed in a suspension of recombinant Agrobacterium and infected by shaking at 180 rpm for 8 min. They were then transferred to a co-culture medium and co-cultured at 25°C in the dark for 3 days.

[0050] (4) Induction of resistant callus and plant regeneration After co-culture, the embryos were transferred to a selection medium to induce the formation of resistant callus, and the medium was changed every 2 weeks.

[0051] The obtained resistant callus tissue was transferred to a regeneration medium and bud differentiation was induced under light conditions. After the regenerated plants grew, they were transferred to a rooting medium and cultured until the root system was well developed before being transplanted into the soil to continue growing.

[0052] Positive transgenic plants were identified using molecular biology methods such as PCR.

[0053] (5) Screening of homozygous lines and seed harvesting The transgenic wheat plants that tested positive were cultured for two generations in a greenhouse, and the homozygous transgenic wheat line TaFIPR-D was obtained through PCR verification and screening. OE -1 and TaFIPR-D OE -2.

[0054] Harvest and preserve the seeds of homozygous transgenic plants; harvest the seeds of normal wheat plants using conventional self-pollination methods.

[0055] The PCR verification procedure is as follows: Genomic DNA was extracted from the transgenic lines and wild-type wheat Fielder using CTAB reagent, and PCR amplification was performed using the corresponding primers. Figure 1A PCR products were detected by agarose gel electrophoresis. Figure 2 ).

[0056] The corresponding primers are shown below: Primer 1: TaFIPR-D OE -ID-F1:5'-GGATTACACATGGCATGGAT-3' (SEQ ID NO:4); TaFIPR-D OE -ID-R1:5'-CTGCAGGCATGCAAGCTTAC-3' (SEQ ID NO:5); Primer 2: TaFIPR-D OE -ID-F2: 5'-CATGAAGGACGTGCTGCACA-3' (SEQ ID NO: 6); TaFIPR-D OE -ID-R2: 5'-GTGGTCTCTCTTTCGTGGG-3' (SEQ ID NO: 7); Primer 3: TaARF-F: 5'-GCTCTCCAACAACATTGCCAAC-3' (SEQ ID NO: 8); TaARF-R: 5'-GCTTCTGCCTGTCACATACGC-3' (SEQ ID NO: 9).

[0057] The experimental conditions for PCR validation are as follows: Prepare the reaction mixture (total volume 25.0 μL): 12.5 μL of 2× Master Mix, 0.5 μL of upstream primer (10 μM), 0.5 μL of downstream primer (10 μM), 0.5~1 μL of template DNA, and the remainder is ddH2O.

[0058] Place the reaction solution on a PCR instrument and proceed with the following reaction conditions: 95℃ pre-denaturation for 3 min; 35 cycles: 95℃ denaturation for 30 s, annealing (Tm -3~5℃) for 30 s, extension at 72℃ (1 kb / min); final extension at 72℃ for 7 min, and incubation at 4℃.

[0059] See results Figure 2 PCR showed that the exogenously introduced TaFIPR-D gene fragment could be detected in the transgenic lines.

[0060] Example 2 (1) Extract the homozygous transgenic line TaFIPR-D using Trizol reagent. OE -1, TaFIPR-D OE Total RNA from wheat Fielder (type 2) and wild-type wheat was purified and reverse transcribed using HiScript III RT SuperMix for qPCR (+gDNAwiper) reverse transcriptase to obtain cDNA.

[0061] (2) Using TaTUBB as an internal reference gene, the expression level of the TFIPR-D gene was detected by real-time quantitative PCR using real-time fluorescence quantitative PCR primers: The ABI Quant Studio 3 real-time quantitative PCR instrument was used, employing ChamQ™ SYBR Color qPCR Master Mix reagents. Results analysis utilized a threshold method to quantify the real-time quantitative PCR results. A fluorescence threshold was set, and the cycle number (Ct) at that threshold was determined. Based on the Ct value, the relative gene expression level was calculated. The specific steps were as follows: a. Calculate the ΔCt values ​​for the target gene and the internal reference gene: ΔCt = Ct (target gene) - Ct (internal reference gene).

[0062] b. Calculate the ΔCt value between the treatment group and the control group: ΔCt = ΔCt (treatment group) - ΔCt (control group).

[0063] c. Calculate relative expression levels: 2 -ΔΔCt .

[0064] The primer sequences used for real-time quantitative PCR are as follows: Primer 4: TaTUBB-F: 5'-CGAGTTCTTGCCGAGGTCCGG-3' (SEQ ID NO: 10); TaTUBB-R: 5'-GGAACAGCACCTCAGGGCAC-3' (SEQ ID NO: 11); Primer 5: TaFIPR-qRT-F: 5'-GGTGACGATCGATCTGACCT-3' (SEQ ID NO: 12); TaFIPR-qRT-R: 5'-CGAGTTCTTGCCGAGGTCCGG-3' (SEQ ID NO: 13).

[0065] The experimental conditions for real-time quantitative PCR are as follows: Prepare the reaction mixture (total volume 20.0 μL): 10.0 μL of 2 × ChamQ Universal SYBR qPCRMaster Mix, 0.4 μL of primer 4 (10 μM), 0.4 μL of primer 5 (10 μM), 1 μL of template cDNA, and the remainder is ddH2O.

[0066] Place the prepared PCR reaction solution on a Realtime PCR instrument for PCR amplification. The reaction conditions are as follows: pre-denaturation at 93℃ for 2 minutes, followed by denaturation at 93℃ for 1 minute, annealing at 55℃ for 1 minute, extension at 72℃ for 1 minute, for a total of 40 cycles, and a final extension at 72℃ for 7 minutes.

[0067] The results are as follows Figure 3 As shown, the expression level of the TaFIPR-D gene is extremely low in wild-type wheat, while the expression level of TaFIPR-D is significantly increased in the transgenic wheat lines of this application compared with wild-type wheat.

[0068] Example 3 (1) The FaFIP polypeptide shown in SEQ ID NO:3 was artificially synthesized and a 100 nM polypeptide TaFIP solution was prepared with pure water; (2) Healthy wheat seeds obtained from the homozygous transgenic line in Example 1 were placed in vermiculite and cultured at a temperature of 25°C and a photoperiod of 12h / 12h. After one week, wheat leaves about 1 cm long were soaked overnight in water containing 0.1wt% Tween-20. (3) On the second day, after soaking the wheat leaves in a 100 nM polypeptide TaFIP solution for 10 to 20 minutes, the wheat leaves were quickly frozen in liquid nitrogen. (4) Extract the protein from wheat leaves after treatment in step (3) and use Western Blot to detect the activation level of MAPK in wheat leaves; wherein, the protein gel concentration used in the Western Blot experiment is 10wt%, the blocking solution is 5wt% skim milk powder, the primary antibody is MAPK phosphorylation antibody (4370S, cell signaling technology, 1:2000 dilution); the secondary antibody is rabbit antibody (7074S, cell signaling technology, 1:5000 dilution); the chemiluminescent membrane is stained with Coomassie brilliant blue, rinsed with decolorizing solution, dried and photographed, and used as internal control; the band used as internal control after staining is Ribulose-1,5-bisphosphate carboxylase / oxygenase, usually abbreviated as RuBisCO (RBC).

[0069] The results are as follows Figure 4 As shown, compared with wild-type wheat, the activity of MAPK in transgenic wheat lines induced by the peptide TaFIP was significantly enhanced, indicating that overexpression of the TaFIPR-D gene in wheat can enhance wheat's recognition of immune-resistant peptides.

[0070] Example 4 (1) Take healthy wheat seeds obtained from the homozygous transgenic wheat line in Example 1, disinfect them in 70wt% ethanol aqueous solution for 1 minute, rinse them 2-3 times with distilled water, and finally soak them in water overnight; (2) On the second day, place the wheat seeds on absorbent paper and keep them moist for 3 days to germinate so that the wheat coleoptile can grow. (3) The homozygous transgenic line TaFIPR-D was soaked in 100 nM polypeptide TaFIP solution. OE -1, TaFIPR-D OE -2 and coleoptiles of wild-type wheat Fielder; (4) Using TaTUBB as an internal reference gene, the expression level of TFIPR-D gene in each wheat line before and after treatment was detected in accordance with Example 2; (5) The WAK2, RLP6, and WRKY24 genes can participate in plant disease resistance processes by recognizing pathogens and regulating gene transcription. See the references for details. [1] Nabi Z, Manzoor S, Nabi SU, et al. Pattern-Triggered Immunity and Effector-Triggered Immunity: crosstalk and cooperation of PRR and NLR-mediated plant defense pathways during host-pathogen interactions[J]. Physiology and Molecular Biology of Plants, 2024(4):30. [2] Sun Y, Wang Y, Zhang X, et al. Plant receptor-like protein activation by a microbial glycoside hydrolase[J]. Nature, 2022, 610:335-342. [3] Javed T, Gao SJ. WRKY transcription factors in plant defense[J]. Trends in Genetics, 2023, 39(10):787-801. These three genes are marker genes for plant immune activation, used to detect the intensity of immune activation in TaFIPR-D transgenic plants.

[0071] The primer sequences used for real-time quantitative PCR are as follows: Primer 4: TaTUBB-F: 5'-CGAGTTCTTGCCGAGGTCCGG-3' (SEQ ID NO: 10); TaTUBB-R: 5'-GGAACAGCACCTCAGGGCAC-3' (SEQ ID NO: 11); Primer 6: TaWAK2-qRT-F: 5'-CGGCTCAGCTTGTGGCGC-3' (SEQ ID NO: 14); TaWAK2-qRT-R: 5'-CTTCGCCTTCGAGGGACAGC-3' (SEQ ID NO: 15); Primer 7: TaWRKY24-qRT-F: 5'-TCCTCGACTCGCCGGTCCTCC-3' (SEQ ID NO: 16); TaWAKY24-qRT-R: 5'-AGGAGGACGCGGTCGTGGTG-3'; (SEQ ID NO: 17); Primer 8: TaRLP6-qRT-F: 5'-AGAGAAGAAGTATATGTGC-3'; (SEQ ID NO: 18); TaRLP6-qRT-R: 5'-AGCGTGCAGGGACCTGCTGC-3' (SEQ ID NO: 19).

[0072] The results are as follows Figure 5 As shown, the expression levels of plant immune-related genes such as WAK2, RLP6, and WRKY24 induced by the peptide TaFIP in plants overexpressing the TaFIPR-D gene were significantly higher than those in the wild type. The experimental results indicate that the activation level of immune resistance was significantly enhanced in transgenic wheat, thus proving that overexpression of the TaFIPR-D gene can enhance wheat's immune recognition ability against the peptide TaFIP, directly reflecting the correlation between the TaFIPR-D gene and plant immune activation.

[0073] Example 5 (1) Cultivate homozygous transgenic wheat plants in a greenhouse for two months until they reach the heading and flowering stage; (2) Prepare a concentration of 1×10 5 Fusarium graminearum spore suspension was used to inoculate wheat ears with Fusarium graminearum spore suspension, and the wheat ears were bagged and moistened at 25°C. (3) Two weeks after inoculation, the plants were phenotypically observed, the number of diseased spikelets was counted, and the proportion of diseased spikelets was calculated to evaluate the occurrence of the disease.

[0074] The results are as follows Figure 6A , Figure 6B As shown, this indicates that increasing the expression level of the receptor-like kinase protein TaFIPR in wheat cells can effectively enhance wheat's resistance to Fusarium graminearum.

[0075] Example 6 (1) Take healthy wheat seeds obtained from the homozygous transgenic line in Example 1, disinfect them in 70wt% ethanol for 1 minute, rinse them 3 times with distilled water, and finally soak them in water overnight. (2) On the second day, place the wheat seeds on absorbent paper and keep them moist for 3 days to germinate so that the wheat coleoptile can grow. (3) Take a block of Fusarium graminearum and inoculate it on the coleoptile of wheat. Place it horizontally and keep it moist for two days. Take a picture and measure the length of the lesion on the coleoptile.

[0076] The results are as follows Figure 7A , Figure 7B As shown, this indicates that increasing the expression level of the receptor-like kinase protein TaFIPR in wheat cells can effectively enhance wheat's resistance to Fusarium graminearum.

[0077] The embodiments described above are merely illustrative of several implementation methods of this application, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this application, and these all fall within the protection scope of this application. Therefore, the protection scope of this patent application should be determined by the appended claims.

Claims

1. The application of the TaFIPR-D gene or biomaterials containing the TaFIPR-D gene in enhancing plant resistance to fungal diseases, characterized in that, The nucleotide sequence of the TaFIPR-D gene is shown in SEQ ID NO:

1.

2. The application according to claim 1, characterized in that, The TaFIPR-D gene encodes a receptor-like kinase protein, TaFIPR, the amino acid sequence of which is shown in SEQ ID NO:

2.

3. The application according to claim 2, characterized in that, By overexpressing the TaFIPR-D gene in the cells of the plant, the level of the receptor-like kinase protein TaFIPR is upregulated.

4. The application according to claim 1, characterized in that, The biomaterial is selected from any one of the following: (a) An expression cassette containing the TaFIPR-D gene; (b) A recombinant vector containing the TaFIPR-D gene; (c) Recombinant microorganisms containing the TaFIPR-D gene.

5. The application according to claim 1, characterized in that, This includes introducing the TaFIPR-D gene as described in claim 1 into the genome of a recipient plant to obtain a transgenic plant; the transgenic plant is capable of overexpressing the TaFIPR-D gene.

6. The application according to claim 5, characterized in that, Also includes: The transgenic plant was treated with the polypeptide TaFIP.

7. The application according to claim 6, characterized in that, The amino acid sequence of the polypeptide FaFIP is shown in SEQ ID NO:

3.

8. The application according to claim 6, characterized in that, A solution of the polypeptide TaFIP was prepared in advance using a solvent, and then the solution was applied to the transgenic plant. The concentration of the solution is 1 nM ~ 1 μM.

9. The application according to claim 5, characterized in that, The recipient plant is wheat; The fungal disease resistance includes resistance to Fusarium graminearum and / or Fusarium pseudograminearum infection.

10. A method for improving wheat resistance to fungal diseases, characterized in that, include: The TaFIPR-D gene was introduced into the genome of recipient wheat via an expression vector and engineered Agrobacterium to obtain transgenic wheat; the nucleotide sequence of the TaFIPR-D gene is shown in SEQ ID NO: 1.

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