A soft tissue mill

Abstract

The application discloses a kind of soft tissue grinders, belong to the technical field of tissue extraction.The grinder is based on conventional tissue container, container cover and grinding mechanism, additional cooling device and liquid nitrogen pipeline cooling piece arranged around grinding groove, both are detachably connected by connecting piece with quick connector;Tissue container and container cover are sealed and connected using stepped thread, and the grinding mechanism is a grinding hammer with anti-slip raised handle, and the device also configures counterweight base to improve stability.Cooling device is a liquid nitrogen circulating device, including liquid nitrogen tank, circulating pump, solenoid valve and controller with built-in AI module, which can intelligently monitor and control liquid nitrogen flow, and the liquid nitrogen tank is equipped with a liquid injection pipe and an electronic scale to realize liquid nitrogen replenishment and remaining amount monitoring.The application realizes low-temperature environment in the grinding groove through liquid nitrogen circulation, making soft tissue harden and easy to grind, and preventing sample degradation throughout the low-temperature process.The device is convenient to disassemble and assemble, easy to operate, and efficient in grinding, suitable for soft tissue sample pretreatment in the field of biomedicine.

Description

Technical Field

[0001] This invention relates to the field of tissue extraction technology, and more specifically, to a soft tissue abrasive. Background Technology

[0002] In biomedical research, to fully understand the biological information of tissue specimens, it is essential to extract RNA, DNA, or proteins with maximum integrity to further understand their expression. Unlike cell samples, tissues, due to their toughness and rigidity, cannot be quickly and thoroughly ground or pulverized, leading to RNA degradation or incomplete extraction during the process, thus affecting the interpretation of results. Therefore, there is an urgent need for a soft tissue grinder to help quickly and thoroughly pulverize tissue blocks, followed by the addition of RNA or protein extraction solutions, to facilitate the rapid, efficient, and complete extraction of biological information from the tissue, creating conditions for accurately analyzing the expression differences of different genes and proteins. Summary of the Invention

[0003] The present invention aims to provide a soft tissue grinder to solve the problem of how to quickly and thoroughly pulverize tissue blocks.

[0004] To achieve the above objectives, the specific plan is as follows:

[0005] A soft tissue abrasive includes a tissue container, a container lid, and an abrasive mechanism, as well as a cooling device and a cooling component. The tissue container has an abrasive groove in the middle, and a cooling component is provided inside the tissue container. The cooling component is arranged around the abrasive groove and is detachably connected to the cooling device via a connector. The top of the abrasive groove has a first step, and the outer wall surface of the first step has a first thread. The container lid has a through hole in the middle, and one end of the lid has a second step. The inner wall surface of the second step has a second thread, and the first thread and the second thread are connected to fix the container lid to the tissue container.

[0006] Furthermore, the cooling components are liquid nitrogen pipelines.

[0007] Furthermore, the cooling device is a liquid nitrogen circulation device, including a liquid nitrogen tank, a liquid nitrogen circulation pump, a fixed pipe, a solenoid valve, and a controller; wherein, the liquid nitrogen circulation pump is installed inside the liquid nitrogen tank, the fixed pipe is connected to the liquid nitrogen circulation pump, and the fixed pipe is equipped with a solenoid valve; the liquid nitrogen tank is also equipped with a controller, which is connected to the solenoid valve through a circuit.

[0008] Furthermore, the connector is equipped with a quick-connect fitting.

[0009] Furthermore, an injection pipe is installed above the liquid nitrogen tank.

[0010] Furthermore, an electronic scale is installed at the bottom of the liquid nitrogen tank.

[0011] Furthermore, the controller has a built-in AI module for real-time monitoring of liquid nitrogen flow changes and for controlling the solenoid valve according to user-preset safety values.

[0012] Furthermore, the grinding mechanism is a grinding hammer, and the grinding hammer has a handle on top.

[0013] Furthermore, anti-slip protrusions are provided around the outer wall of the handle.

[0014] Furthermore, it also includes a base, with the tissue container detachably mounted on the base.

[0015] In summary, the present invention has the following advantages over the prior art:

[0016] (1) The present invention sets a cooling component around the grinding tank in the tissue container and uses a liquid nitrogen circulation device to achieve continuous and uniform cooling of the grinding tank, so that the soft tissue to be ground can be hardened and brittle rapidly in a low temperature environment, greatly reducing the grinding resistance. The staff can easily grind the soft tissue into powder with the grinding hammer. Compared with the traditional room temperature grinding method, it not only grinds more thoroughly and leaves no tissue clumps, but also greatly shortens the grinding operation time and improves the overall efficiency of sample pretreatment.

[0017] (2) The liquid nitrogen circulation device realizes real-time monitoring and intelligent control of liquid nitrogen flow through the AI ​​module built into the controller. It can automatically adjust the opening and closing degree of the solenoid valve according to the flow disturbance during the grinding process, ensuring the stable cooling effect of the cooling component and maintaining the preset low temperature environment in the grinding tank throughout the process. This fundamentally avoids the degradation of biological samples such as RNA, DNA, and protein caused by temperature rise during the grinding process, ensuring the integrity of the sample and the accuracy of subsequent detection and analysis results.

[0018] (3) The tissue container and the cooling device are detachably and quickly connected by a quick-connect fitting, which facilitates the individual handling, cleaning and disinfection of the tissue container; the tissue container and the container cap are connected by a stepped threaded connection, which not only ensures the sealing effect of the grinding tank and reduces the loss of low temperature environment, but also effectively prevents tissue samples from splashing out during the grinding process; the handle of the grinding hammer is equipped with anti-slip protrusions and the base is a solid counterweight structure, which not only improves the stability of hand operation, but also avoids the shaking and displacement of the tissue container during grinding. The staff can complete the grinding with one hand, which greatly improves the convenience of operation; at the same time, the liquid nitrogen tank is equipped with a liquid injection pipe and an electronic scale at the bottom, which can monitor the liquid nitrogen balance in real time and replenish it in time, avoiding liquid nitrogen leakage or interruption, and improving the safety and continuity of the device. Attached Figure Description

[0019] The accompanying drawings, which are included to provide a further understanding of the invention and form part of this invention, illustrate exemplary embodiments of the invention and are used to explain the invention, but do not constitute an undue limitation of the invention. In the drawings:

[0020] Figure 1 A schematic diagram of the overall structure of a soft tissue grinder;

[0021] Figure 2 A top view of a soft tissue abrasive;

[0022] Figure 3 A cross-sectional view of a soft tissue abrasive.

[0023] Figure 4 An exploded view of a soft tissue grinder.

[0024] The above figures include the following reference numerals:

[0025] 1. Organizing container; 11. Grinding tank; 12. Cooling component; 13. First step; 131. First thread; 2. Container cap; 21. Through hole; 22. Second step; 221. Second thread; 3. Grinding hammer; 31. Handle; 311. Anti-slip protrusion; 4. Liquid nitrogen circulation device; 41. Liquid nitrogen tank; 42. Liquid nitrogen circulation pump; 43. Fixing pipe; 44. Solenoid valve; 45. Controller; 451. Wiring; 46. Injection pipe; 5. Connector; 51. Quick connector; 6. Electronic scale; 7. Base. Detailed Implementation

[0026] It should be noted that, unless otherwise specified, the embodiments and features described in the present invention can be combined with each other. The present invention will now be described in detail with reference to the accompanying drawings and embodiments.

[0027] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments of the invention. As used herein, the singular form may also include the plural form unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0028] Unless otherwise specifically stated, the relative arrangement, numerical expressions, and values ​​of the components and steps set forth in these embodiments do not limit the scope of the invention. It should also be understood that, for ease of description, the dimensions of the various parts shown in the drawings are not drawn to actual scale. Techniques, methods, and devices known to those skilled in the art may not be discussed in detail, but where appropriate, such techniques, methods, and devices should be considered part of the specification. In all examples shown and discussed herein, any specific values ​​should be interpreted as merely exemplary and not as limitations. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters in the following figures denote similar items; therefore, once an item is defined in one figure, it need not be further discussed in subsequent figures.

[0029] See Figures 1 to 4 As shown, the present invention provides a soft tissue abrasive. Similar to the prior art, the device mainly consists of three parts: a tissue container 1, a container lid 2, and an abrasive mechanism. Unlike the prior art, the tissue container 1 has an abrasive groove 11 in the middle, a first step 13 at the top of the abrasive groove 11, and a first thread 131 on the outer wall of the first step 13. The tissue container 1 also has a cooling component 12, which surrounds the abrasive groove 11 and is detachably connected to the cooling device via a connector 5. The container lid 2 has a through hole 21 in the middle, and a second step 22 at one end. A second thread 221 is provided on the inner wall of the second step 22. The first thread 131 and the second thread 221 are connected to fix the container lid 2 on the tissue container 1.

[0030] In use, first activate the cooling device to cool the grinding tank 11 using the cooling component 12, maintaining it at a low temperature. Then, place the soft tissue to be ground into the tissue container 1, allowing it to harden and become brittle in the low-temperature environment to facilitate grinding. Subsequently, use the grinding mechanism to grind the hardened soft tissue.

[0031] As a preferred option, the cooling component 12 is a liquid nitrogen pipeline.

[0032] As a preferred embodiment, the cooling device is a liquid nitrogen circulation device 4, which includes a liquid nitrogen tank 41, a liquid nitrogen circulation pump 42, a fixed pipe 43, a solenoid valve 44, and a controller 45. The liquid nitrogen circulation pump 42 is installed inside the liquid nitrogen tank 41, the fixed pipe 43 is connected to the liquid nitrogen circulation pump 42, and the solenoid valve 44 is installed on the fixed pipe 43. The controller 45 is also installed on the liquid nitrogen tank 41, and the controller 45 is connected to the solenoid valve 44 through a line 451.

[0033] During use, liquid nitrogen circulates in the liquid nitrogen pipeline through the liquid nitrogen circulation device 4. During this process, the liquid nitrogen in the liquid nitrogen pipeline can always be kept full, so that the grinding tank 11 is always kept at a low temperature, which makes it convenient to use the grinding mechanism to grind soft tissue into powder.

[0034] As a preferred embodiment, the connector 5 is provided with a quick connector 51, which can be quickly connected to the fixing tube 43, thereby allowing the tissue container 1 to be detachably connected to the cooling device.

[0035] As a preferred embodiment, a liquid injection pipe 46 is provided above the liquid nitrogen tank 41 for replenishing the liquid nitrogen in the liquid nitrogen tank 41.

[0036] As a preferred option, an electronic scale 6 is provided at the bottom of the liquid nitrogen tank 41, and the remaining liquid nitrogen in the liquid nitrogen tank 41 is calculated by the weight change of the electronic scale 6.

[0037] As a preferred option, the grinding mechanism is a grinding hammer 3, and the top of the grinding hammer 3 is provided with a handle 31 for easy handling by the staff.

[0038] As a preferred option, the outer wall of the handle 31 is provided with anti-slip protrusions 311 to facilitate the user's grip and prevent the grinding hammer 3 from slipping due to sweaty hands when using it.

[0039] As a preferred option, since the pressure generated during the use of the grinding device may affect the liquid nitrogen flow rate in the liquid nitrogen pipeline, the controller 45 of this device has a built-in AI module. The AI ​​module can monitor the changes in liquid nitrogen flow rate in real time and control the solenoid valve 44 according to the user-preset safety value, thereby keeping the liquid nitrogen flow rate in the liquid nitrogen pipeline within a reasonable range and ensuring the cooling effect of the grinding tank 11.

[0040] As a preferred embodiment, a base 7 is also included, with the tissue container 1 detachably mounted to the base 7. During use, the base 7 stabilizes the tissue container 1, thereby reducing disturbance to the liquid nitrogen flow rate within the liquid nitrogen pipeline. Furthermore, the base 7 allows the user to operate the device with one hand during tissue grinding.

[0041] The method of using this device is as follows:

[0042] Method of using the present invention

[0043] S1, Device assembly: Place the tissue container 1 stably on the base 7, and quickly connect the cooling component 12 inside the tissue container 1 to the fixed pipe 43 of the liquid nitrogen circulation device 4 through the quick connector 51 on the connector 5. Check the connection seal to prevent liquid nitrogen leakage.

[0044] S2, Low-temperature precooling: Sufficient liquid nitrogen is added to the liquid nitrogen tank 41 through the injection pipe 46, the liquid nitrogen circulation pump 42 is started, the controller 45 is automatically turned on and the liquid nitrogen flow rate is monitored through the AI ​​module, the liquid nitrogen enters the cooling component 12 through the fixed pipe 43 and circulates, precooling the grinding tank 11, so that a stable low-temperature environment is formed in the grinding tank 11.

[0045] S3, Sample Placement: After the grinding tank 11 reaches the preset low temperature, open the container lid 2, quickly place the soft tissue sample to be ground into the grinding tank 11, and immediately screw the container lid 2 onto the tissue container 1 to reduce the loss of low temperature environment.

[0046] S4, Low-temperature grinding: The operator holds the handle 31 of the grinding hammer 3 and inserts the hammer body of the grinding hammer 3 into the grinding tank 11 through the through hole 21 to repeatedly grind the soft tissue that has hardened and become brittle at low temperature until the tissue is ground into powder. During the grinding process, the AI ​​module of the controller 45 adjusts the liquid nitrogen flow rate in real time to ensure the low-temperature stability of the grinding tank 11.

[0047] S5, Subsequent Operations: After grinding is complete, turn off the liquid nitrogen circulation pump 42, unscrew the container cap 2, take out the ground tissue powder, and then add RNA and protein extraction solution for subsequent biological sample extraction; disassemble the quick connector 51 to separate the tissue container 1 from the liquid nitrogen circulation device 4, and clean and disinfect the tissue container 1, container cap 2 and grinding hammer 3 for easy reuse.

[0048] S6, Liquid nitrogen replenishment: During use, the weight change of the liquid nitrogen tank 41 can be viewed in real time through the electronic scale 6. When the liquid nitrogen is insufficient, liquid nitrogen can be replenished in time through the injection pipe 46.

[0049] Structures not described in detail in this embodiment are all processed, installed and connected in accordance with conventional methods in the art, and the materials of each component can be selected as medical-grade materials that are resistant to low temperature and corrosion according to actual use requirements to ensure the biosafety and service life of the device.

[0050] For ease of description, spatial relative terms such as "above," "on top of," "on the upper surface of," "above," etc., are used herein to describe the spatial positional relationship of a device or feature as shown in the figures to other devices or features. It should be understood that spatial relative terms are intended to encompass different orientations in use or operation beyond the orientation of the device as described in the figures. For example, if the device in the figures were inverted, a device described as "above" or "on top of" other devices or structures would subsequently be positioned as "below" or "under" other devices or structures. Thus, the exemplary term "above" can include both "above" and "below." The device may also be positioned in other different ways (rotated 90 degrees or in other orientations), and the spatial relative descriptions used herein will be interpreted accordingly.

[0051] Furthermore, it should be noted that the use of terms such as "first" and "second" to define components is merely for the purpose of distinguishing the corresponding components. Unless otherwise stated, the above terms have no special meaning and therefore should not be construed as limiting the scope of protection of this invention.

[0052] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A soft tissue abrasive, comprising a tissue container (1), a container lid (2), and an abrasive mechanism, characterized in that, It also includes a cooling device and a cooling component (12); the tissue container (1) is provided with a grinding groove (11) in the middle position, and the tissue container (1) is provided with a cooling component (12) inside. The cooling component (12) is arranged around the grinding groove (11) and is detachably connected to the cooling device through a connector (5); the top of the grinding groove (11) is provided with a first step (13), and the outer wall surface of the first step (13) is provided with a first thread (131); the container cover (2) is provided with a through hole (21) in the middle position, and one end of it is provided with a second step (22). The inner wall surface of the second step (22) is provided with a second thread (221). The first thread (131) is connected to the second thread (221) so that the container cover (2) is fixed on the tissue container (1).

2. The soft tissue abrasive according to claim 1, characterized in that, The cooling component (12) is a liquid nitrogen pipeline.

3. The soft tissue abrasive according to claim 1, characterized in that, The cooling device is a liquid nitrogen circulation device (4), including a liquid nitrogen tank (41), a liquid nitrogen circulation pump (42), a fixed pipe (43), a solenoid valve (44), and a controller (45); wherein, the liquid nitrogen circulation pump (42) is installed inside the liquid nitrogen tank (41), the fixed pipe (43) is connected to the liquid nitrogen circulation pump (42), and the solenoid valve (44) is provided on the fixed pipe (43); the controller (45) is also provided on the liquid nitrogen tank (41), and the controller (45) is connected to the solenoid valve (44) through a line (451).

4. The soft tissue abrasive according to claim 1, characterized in that, The connector (5) is provided with a quick connector (51).

5. The soft tissue abrasive according to claim 3, characterized in that, The liquid nitrogen tank (41) is equipped with an injection pipe (46) above it.

6. The soft tissue abrasive according to claim 3, characterized in that, An electronic scale (6) is installed at the bottom of the liquid nitrogen tank (41).

7. The soft tissue abrasive according to claim 3, characterized in that, The controller (45) has a built-in AI module for real-time monitoring of liquid nitrogen flow rate changes and for controlling the solenoid valve (44) according to the user-preset safety value.

8. The soft tissue abrasive according to claim 1, characterized in that, The grinding mechanism is a grinding hammer (3), and the top of the grinding hammer (3) is provided with a handle (31).

9. The soft tissue abrasive according to claim 8, characterized in that, The outer wall surface of the handle (31) is provided with anti-slip protrusions (311).

10. The soft tissue abrasive according to any one of claims 1 to 9, characterized in that, It also includes a base (7), and the tissue container (1) is detachably mounted together with the base (7).

Images