Use of a haplotype molecular marker associated with sheep body weight and muscle fiber diameter
CN122256535BActive Publication Date: 2026-08-28INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
Patent Information
- Application Number
- CN202610737353.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2026-05-27
- Publication Date
- 2026-08-28
- Estimated Expiration
- 2046-05-27
AI Technical Summary
Technical Problem
[0004]为解决以上问题，本发明提供一种与体重相关的单倍型分子标记在鉴定绵羊肌纤维直径中的应用，从而解决传统技术中肌纤维直径测定需依赖屠宰后组织采样，无法应用于活体早期选育，导致育种周期冗长、世代间隔长，育种效率低下的问题
Benefits of technology
本发明提供一种与绵羊体重和肌纤维直径相关的单倍型分子标记的应用，所述单倍型分子标记为1）和2）：1）SEQ ID NO.1所示的核苷酸序列；所述SEQ ID NO.1的第101bp位点处的核苷酸为T或C。2）SEQ ID NO.2所示的核苷酸序列；所述SEQ ID NO.2的第101bp位点处的核苷酸为C或T。所述应用是指以下（1）~（4）中的任意一种：（1）鉴定绵羊的体重；（2）鉴定绵羊的肌纤维直径；（3）提高绵羊后代的体重；（4）降低绵羊后代的肌纤维直径。本发明首次揭示了绵羊体重相关单倍型分子标记与肌纤维直径之间的强关联性，通过检测SEQ IDNO.1和SEQ ID NO.2第101bp位点的单倍型组合TTCC，即可在活体状态下准确鉴定出肌纤维直径较小的绵羊个体，避免了传统方法依赖屠宰后组织学检测的不可逆性，实现了对肌纤维直径的早期、无损、高通量筛选。该方法操作简便、结果稳定，可直接应用于育种亲本选择，通过保留TTCC单倍型个体作为亲本，能够有效降低后代的肌纤维直径，改善羊肉嫩度等品质性状。本发明突破了体重表型间接选择的局限性，为绵羊肉质性状的遗传改良提供了可靠的分子标记和高效的技术路径，显著缩短育种周期，提升选育精准度和经济效益。
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Abstract
The present application relates to the technical field of genetic breeding, and particularly relates to application of a haplotype molecular marker related to sheep weight and muscle fiber diameter, wherein the haplotype molecular marker is 1) and 2): 1) a nucleotide sequence shown in SEQ ID NO. 1; the nucleotide at the 101bp site of the SEQ ID NO. 1 is T or C; 2) a nucleotide sequence shown in SEQ ID NO. 2; the nucleotide at the 101bp site of the SEQ ID NO. 2 is C or T. Through the significant SNP site related to weight, early identification of the muscle fiber diameter of live sheep is realized, which can be used for assisting breeding to reduce the muscle fiber diameter of offspring, has the characteristics of non-damage, high efficiency and precision, and can effectively shorten the breeding cycle and improve the growth performance of sheep and the efficiency of meat quality improvement.
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Citation Information
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