A fluorescence detection system for a nucleic acid detection microfluidic chip and application thereof

Abstract

This invention relates to the field of nucleic acid detection technology, specifically to a fluorescence detection system for a microfluidic chip used in nucleic acid detection and its application. Specifically, this invention provides a fluorescence detection system and method for a centrifugal nucleic acid detection microfluidic chip. The design concept of the fluorescence detection system of this invention is as follows: the excitation optical path and the microfluidic chip are synchronously fixed on a chip fixing module, keeping the excitation optical path and the detection chamber relatively stationary and unaffected by centrifugal rotation, ensuring that the excitation light always accurately acts on the detection chamber; a one-to-one correspondence between the excitation optical path and the detection chamber is adopted to achieve synchronous excitation of multiple chambers, eliminating the need for expensive optical scanning or electric adjustment mechanisms to achieve complex synchronous control; by placing a camera above the chip fixing module and equipping it with a dedicated detection filter, and using orthogonal optical paths, the capture efficiency and signal-to-noise ratio of the fluorescence signal are improved, thereby solving the problems of unstable excitation, low detection efficiency, and poor signal capture in existing technologies.

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