A method for preventing and controlling plant diseases, insect pests and weeds in green gordon euryale planting
By employing a synergistic control method combining compound biocontrol agents, natural enemy insects, and plant-derived pheromones, along with waste recycling, the chemical dependence and environmental issues related to pests, diseases, and weeds in gorgon fruit cultivation have been resolved. This approach achieves green control and resource recycling throughout the entire growth cycle, resulting in a win-win situation for both ecological and economic benefits.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- HUAIAN QIANRUN AGRICULTURAL TECHNOLOGY CO LTD
- Filing Date
- 2026-03-19
- Publication Date
- 2026-06-26
AI Technical Summary
The current control of diseases, pests and weeds in gorgon fruit cultivation relies on chemical pesticides, which leads to excessive pesticide residues, damage to aquatic ecosystems, increased pesticide resistance in pests, and unstable biological control effects. Furthermore, waste disposal during the cultivation process is not environmentally friendly, making it difficult to achieve comprehensive control throughout the entire growth period.
A multi-dimensional synergistic control method using compound biocontrol agents, natural enemy insects, and plant-derived resistance inducers, combined with waste recycling, is adopted to construct a synergistic control system throughout the entire growth period, including field pretreatment, seedling stage control barriers, stress resistance enhancement during the growth period, and waste recycling.
This approach enables green pest and weed control throughout the entire growth cycle of water chestnut, avoiding the use of chemical pesticides, reducing production costs, minimizing agricultural non-point source pollution, and ensuring that the product meets green food standards, thus achieving a win-win situation for both ecological and economic benefits.
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Abstract
Description
Technical Field
[0001] This invention belongs to the field of green planting technology for aquatic vegetables, specifically relating to a biological control method for diseases, pests and weeds in green planting of water chestnut. Background Technology
[0002] Euryale ferox is a unique aquatic economic crop in my country, used for both food and medicine. It is widely cultivated in the Yangtze River and Huai River basins. Its kernels are rich in starch, protein, and flavonoids, possessing both edible and medicinal value. Euryale ferox grows entirely in a flooded environment, and the high temperature and humidity in the field make it highly susceptible to frequent outbreaks of diseases, pests, and weeds. Major diseases include stem rot, leaf spot, and anthracnose; major pests include beet armyworm, aphids, and golden apple snails; and major weeds include duckweed, pondweed, and barnyard grass. These diseases, pests, and weeds can reduce euryale ferox yield by 20%-40%, and in severe cases, even lead to total crop failure.
[0003] Currently, pest and weed control in gorgon fruit cultivation still relies primarily on chemical control, depending on chemical fungicides, insecticides, and herbicides for emergency treatment. This approach has significant technical shortcomings: First, the long-term overuse of chemical pesticides leads to excessive pesticide residues in gorgon fruit products, failing to meet the requirements for green food production. It also damages aquatic ecosystems and exacerbates agricultural non-point source pollution. Second, pathogens and pests are becoming increasingly resistant to pesticides, leading to a vicious cycle of resistance and increased pesticide use, resulting in a continuous decline in control efficacy. Third, existing biological control technologies are simplistic, often using single microbial agents or single natural enemy insects for localized control. A comprehensive control system covering the entire gorgon fruit growth cycle has not been established. Microbial agents have poor field colonization capabilities and insufficient stability in viable cell counts, while natural enemy insects offer weak sustained control, resulting in large fluctuations in overall pest and weed control efficacy and an inability to achieve stable control throughout the entire cycle. Fourth, field wastewater, plant residues, and processing byproducts generated during cultivation are not recycled, leading to high waste disposal costs and potential secondary pollution, which does not meet the requirements of modern green and circular agricultural development.
[0004] Based on this, the present invention provides a biological control method for diseases, pests and weeds in green planting of water chestnut, so as to solve the technical problems existing in the prior art.
[0005] In summary, a biological control method for diseases, pests, and weeds in green cultivation of water chestnut needs to be proposed to solve the above problems. Summary of the Invention
[0006] The purpose of this invention is to provide a biological control method for diseases, pests and weeds in the green cultivation of water chestnut, so as to solve the problems mentioned in the background art.
[0007] To achieve the above objectives, the present invention provides the following technical solution: This invention provides a method for biological control of pests, diseases, and weeds in the green cultivation of water chestnut, comprising the following steps: S1. First, conduct background testing and impurity purification on the planting field and irrigation water source. Then, prepare a composite biocontrol agent with set viable bacteria count and carrier pore size through a multi-step process of slant activation, seed liquid propagation, solid fermentation, and carrier adsorption. Simultaneously prepare biocontrol substrate and propagate the matching natural enemy insect population. S2. Based on the number of viable bacteria in the compound biocontrol agent obtained in step S1, determine the ratio of decomposed organic fertilizer to biocontrol substrate. Mix the pretreated decomposed organic fertilizer, biocontrol substrate and compound biocontrol agent, control the water temperature and tillage parameters, and complete the pretreatment of the rhizosphere microenvironment of the planting field. S3. Transplant the pretreated water chestnut seedlings into the field after the pretreatment in step S2 at the set planting density, and simultaneously apply the compound biocontrol agent and natural enemy insects from step S1 at the set amount to control the water depth and light duration, and maintain the stability of water parameters based on the pretreatment status of the field in step S2. S4. Conduct field monitoring of the growth status of the gorgon fruit seedlings and the occurrence of diseases, pests and weeds in step S3 to obtain basic data on seedling control. Then, add the fermentation by-products from the preparation of the compound biocontrol agent in step S1 to prepare plant-derived inducers, and spray and splash them on the gorgon fruit plants and field water. S5. After the treatment in step S4, the compound biocontrol agent, sex pheromone and natural enemy insects from step S1 are added to the gorgon fruit field according to the set feeding frequency and amount. The dissolved oxygen, pH value and plant population density in the water are controlled. The control frequency is adjusted based on the field monitoring data from step S4. Fresh gorgon fruit is then harvested. S6. First, the plant residues and weeds in the field after harvesting in step S5 are treated by biodegradation. Then, the primary product of gorgon fruit is obtained by cleaning, shelling and low-temperature drying. Based on the pathogen content of fresh gorgon fruit in step S5, a natural antibacterial preservative is added to obtain the final product of gorgon fruit. S7. The field aquaculture wastewater generated from the seedling stage in step S3 to the reproductive growth stage in step S5 is adsorbed by the remaining biocontrol substrate carrier in step S1 and then degraded and purified by the compound biocontrol agent, and recycled for field irrigation. The solid waste generated in the treatment process in step S6 is recycled for field base fertilizer after aerobic composting and fermentation. S8. The final product of the gorgon fruit in step S6 was tested by high performance liquid chromatography and plate count method. Based on the amount of natural antibacterial preservative used in step S6, the packaging container and warehouse conditions were selected for product storage. S9. Record the seedling survival rate during the seedling stage in step S3 and the comprehensive control efficacy of diseases, pests and weeds during the reproductive growth stage in step S5. If the rate is lower than the set threshold, adjust the relevant control process parameters. Based on the test results of step S8, regularly calibrate the dosage and timing of biocontrol materials for each growth stage.
[0008] Preferably, the implementation process of step S1 is as follows: S1.1. Select water chestnut varieties with a resistance level of ≥3 for leaf spot and stem rot, clear and level the planting field, remove field residues, weeds and wild snails, and ensure the flatness error of the field is ≤5cm. Filter the irrigation water with sand to remove plankton and solid impurities, and test the pathogen content in the water source to ≤10CFU / mL. S1.2. Preparation of a compound biocontrol agent of Bacillus amyloliquefaciens, Bacillus subtilis, and Trichoderma harzianum: Bacillus amyloliquefaciens and Bacillus subtilis were inoculated into LB slant medium, and Trichoderma harzianum was inoculated into PDA slant medium. The mixture was activated at 28℃ for 24 h. Single colonies were picked and inoculated into the corresponding liquid seed culture medium. The mixture was then amplified by shaking at 180 r / min at 28℃ for 24 h to obtain seed liquids. The three seed liquids were inoculated into a solid fermentation medium at a volume ratio of 3:3:4. The solid fermentation medium consisted of 60 parts by weight of wheat bran, 20 parts by weight of soybean meal, 15 parts by weight of corn flour, 3 parts by weight of glucose, 0.5 parts by weight of magnesium sulfate, and 1.5 parts by weight of potassium dihydrogen phosphate, with a water content of 55% and a pH of 6.8-7.0. Fermentation was carried out at 28℃ for 72 h. After adsorption on a diatomaceous earth carrier and drying at 30℃ for 12 h, a total viable count ≥2×10¹ was obtained. 0 A composite biocontrol agent with CFU / g and a carrier pore size of 0.2-0.5mm is prepared for use. S1.3. A biocontrol substrate was prepared by mixing 5 parts by weight of rice husk, 3 parts by weight of biochar, and 2 parts by weight of vermiculite. The natural enemy insect populations of Trichogramma wasp, Amblyseius mite, and Viviparus sinensis were simultaneously propagated. Trichogramma wasp propagated using rice moth eggs as hosts at a temperature of 25℃ and a relative humidity of 70%. Amblyseius mite propagated using Tyromitra saprophytic mites as food at a temperature of 26℃ and a relative humidity of 85%. Healthy Viviparus sinensis individuals weighing 10-15g were selected and temporarily raised and acclimatized for 7 days before use.
[0009] Preferably, the implementation process of step S2 is as follows: S2.1. The total viable count of the composite biocontrol agent prepared in step S1 is 2 × 10¹ 0 CFU / g, the pretreated well-rotted organic fertilizer and biocontrol substrate were evenly spread on the planting field at a ratio of 9 parts by weight of well-rotted organic fertilizer and 1 part by weight of biocontrol substrate. The well-rotted organic fertilizer was rapeseed cake fertilizer that had been decomposed at high temperature and aerobically, with an organic matter content of ≥45%. S2.2. Add 0.5 parts by weight of the compound biocontrol agent prepared in step S1 to every 100 parts by weight of the mixed material, control the water temperature of the field at 15-25℃, the tillage depth at 15-20cm, and till twice continuously with an interval of 24 hours between each tillage. After tillage, let it stand for 7 days to complete the pretreatment of the rhizosphere microenvironment of the planting field.
[0010] Preferably, the implementation process of step S3 is as follows: S3.1. Before transplanting, soak the roots of the gorgon fruit seedlings in a 500-fold diluted solution of the compound biocontrol agent prepared in step S1 for 30 minutes. After pretreatment in step S2, control the water depth in the field to 10-15 cm and transplant gorgon fruit seedlings at the 4-leaf and 1-heart stage at a planting density of 1.5 m × 2.0 m. At the same time, apply the compound biocontrol agent prepared in step S1 at a rate of 1.5 kg per mu and release 1500 amblyseius mites per mu. S3.2. Based on the rhizosphere microenvironment of the field, maintain stable water depth and temperature, and monitor the pathogens of stem rot and leaf spot diseases in the field weekly during the growth process, as well as the germination density of duckweed and pondweed.
[0011] Preferably, the implementation process of step S4 is as follows: S4.1. Conduct field monitoring once a week on the seedling stage of the water chestnut field in step S3, record the plant growth status, types and numbers of diseases, pests and weeds, and obtain basic data for seedling control. S4.2. Collect the byproduct of the centrifuged supernatant after solid fermentation during the preparation of the compound biocontrol agent in step S1, filter it through a 0.22μm filter membrane for sterilization, and detect that the content of lipopeptide metabolites is ≥500mg / L. Dilute it with water at a volume ratio of 1:500 to prepare a plant-derived inducer. S4.3. Apply the resistant inducer to the field of water chestnut at a rate of 30L per mu, and simultaneously spray the leaves at a rate of 1L per mu. Repeat the treatment twice at 20-25℃, with an interval of 7 days between each treatment.
[0012] Preferably, the implementation process of step S5 is as follows: S5.1. After the treatment in step S4, control the water depth in the water body to 30-50cm, the dissolved oxygen content to ≥5mg / L, and the pH value to 6.5-7.5. Apply the compound biocontrol agent prepared in step S1 at a rate of 1.0kg per mu and once every 15 days. S5.2. Release Trichogramma wasps in two batches at a total of 10,000 wasps per mu. Hang Spodoptera litura pheromone at a density of 200 pheromones per mu. The active ingredient of the pheromone is cis-9,trans-11-tetradecadiene acetate. The slow-release carrier is a rubber stopper. Hang the pheromone at a height 10cm above the canopy of the Euryale ferox plants. At the same time, release Chinese round snails at a rate of 50kg per mu. S5.3. Based on the field monitoring data from step S4, adjust the control frequency, complete the material application from flowering to seed setting of Euryale ferox, and harvest fresh Euryale ferox fruits.
[0013] Preferably, the implementation process of step S6 is as follows: S6.1. After harvesting fresh gorgon fruit in step S5, control the water depth to 20cm and the water temperature to 20-30℃ in the field. Apply the compound biocontrol agent prepared in step S1 at a rate of 2.0kg per mu. Turn the pond once every 10 days to biodegrade plant residues and weeds in the field. The degradation cycle is 30 days. S6.2. The harvested fresh gorgon fruit is cleaned with water, mechanically dehulled, and dried at a low temperature of 40℃ to obtain the initial product of gorgon fruit; S6.3. Add 0.2 parts by weight of eugenol natural antibacterial preservative to every 100 parts by weight of the initial product of fox nuts, and stir evenly to obtain the final product of fox nuts.
[0014] Preferably, the implementation process of step S7 is as follows: S7.1. Collect field aquaculture wastewater generated from the seedling stage in step S3 to the reproductive growth stage in step S5; S7.2. Pass it into the adsorption tower filled with the remaining biochar carrier from step S1 when preparing the biocontrol substrate. The biochar carrier has a particle size of 2-5 mm. After adsorption treatment, the wastewater is connected to the biological purification tank. Add 0.5 parts by mass of the composite biocontrol agent prepared in step S1 per cubic meter of water. Control the hydraulic retention time to 72 h, the dissolved oxygen content of aeration ≥ 4 mg / L, and the chemical oxygen demand of the effluent after degradation and purification ≤ 50 mg / L and ammonia nitrogen ≤ 5 mg / L, and then recycle it for field irrigation. S7.3. Collect the solid waste generated during the treatment of step S6, such as the husks and plant residues after degradation, and compost them aerobically for 30 days. After decomposition, reuse them as base fertilizer in the field.
[0015] Preferably, the implementation process of step S8 is as follows: S8.1. The final product of gorgon fruit obtained in step S6 is tested. Pesticide residues are detected by high performance liquid chromatography, and the total number of pathogens is detected by plate count method. The pesticide residues in the final product of gorgon fruit are controlled to be undetectable, the total number of pathogens is ≤10 CFU / g, and the moisture content is ≤13%. S8.2. Based on the amount of eugenol natural antibacterial preservative added in step S6, seal the qualified product into food-grade polyethylene vacuum packaging bags, each bag containing 5 kg net weight, and store in a cool and ventilated warehouse with the temperature controlled at 0-4℃ and the relative humidity controlled at 45-55%, avoiding light exposure.
[0016] Preferably, the implementation process of step S9 is as follows: S9.1. Record the seedling survival rate during the seedling stage in step S3 and the comprehensive control efficacy of pests, diseases and weeds during the reproductive growth stage in step S5. When the seedling survival rate during the seedling stage is less than 93%, adjust the dosage of compound biocontrol agent in step S2 to 0.7 parts by weight per 100 parts by weight of mixed material, and increase the application rate of compound biocontrol agent in step S3 to 2.0 kg per mu. S9.2. When the comprehensive control efficacy of pests, diseases and weeds during the reproductive growth period is less than 82%, the frequency of supplementary application of the compound biocontrol agent in step S5 is adjusted to once every 10 days. At the same time, 50% of the initial total amount applied in step S5.2 is supplemented with the release of Trichogramma wasps and Atlas seimitus. S9.3. Based on the product testing results of step S8, recalibrate the dosage of the inducer in step S4 and the biocontrol material delivery parameters in step S5 after every 5 batches of production.
[0017] Compared with the prior art, the beneficial effects of the present invention are as follows: The present invention constructs a closed-loop prevention and control system covering the entire chain, including field pretreatment, seedling stage control barrier, growth period stress resistance enhancement, synergistic prevention and control throughout the entire growth period, waste recycling, and dynamic process calibration. Through multi-dimensional synergy of compound biocontrol agents, biocontrol substrates, natural enemy insects, and plant-derived inducers, it achieves green prevention and control of diseases, pests, and weeds throughout the entire growth period of Euryale ferox, without the need for chemical pesticides or herbicides, thus eliminating the risk of pesticide residues from the source. This invention optimizes the preparation process of the compound biocontrol agent. Through the synergistic combination of Bacillus amyloliquefaciens, Bacillus subtilis, and Trichoderma harzianum, it achieves multiple effects including pathogen inhibition, soil improvement, and plant growth promotion. The prepared compound biocontrol agent has a total viable count ≥2×10¹. 0 CFU / g, with strong field colonization ability, and at the same time, the fermentation by-products in the preparation of microbial agents are used to prepare plant-derived inhibitors, realizing the high-value utilization of by-products and reducing production costs; This invention establishes a complete recycling system for planting waste and wastewater. Field breeding wastewater is recycled for irrigation after being adsorbed by biochar and degraded by compound microbial agents. Plant residues and solid waste such as water chestnut shells are recycled for field base fertilizer after being aerobic composting and decomposing. This reduces agricultural non-point source pollution and realizes planting and breeding cycle and ecological sustainability. This invention establishes a dynamic calibration mechanism for process parameters. Based on the seedling survival rate, the comprehensive control efficacy against diseases, pests and weeds during the reproductive growth period, and the final product quality test results, the amount of biocontrol materials and application parameters are dynamically adjusted to ensure stable control effects between batches. All indicators of the final product of Euryale ferox meet the requirements of the NY / T1042-2017 green food standard, achieving both ecological and economic benefits. Detailed Implementation
[0018] Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0019] I. Materials: Unless otherwise specified, all raw materials used in the biological control method for pests, diseases, and weeds in the green cultivation of Euryale ferox in this invention are commercially available agricultural-grade conventional products. Microbial strains: Bacillus amyloliquefaciens, Bacillus subtilis, and Trichoderma harzianum are all commercially available strains that meet the production standards for agricultural microbial agents; Culture medium ingredients: The peptone, yeast extract, agar, and glucose used in LB slant medium, PDA slant medium, and liquid seed medium are all microbial culture grade reagents; the wheat bran, soybean meal, corn flour, magnesium sulfate, and potassium dihydrogen phosphate used in solid fermentation medium are all commercially available agricultural grade products; Planting materials: The gorgon fruit variety is a commercially available variety with a resistance level of ≥3 to leaf spot and stem rot. The decomposed organic fertilizer is high-temperature aerobic decomposed rapeseed cake fertilizer that meets the NY525-2021 standard. The rice husks, biochar, and vermiculite used in the biocontrol substrate are all commercially available conventional products. Supporting materials for pest control: Trichogramma wasps and Amblyseius mites are commercially available natural enemy insects; Chinese round snails are commercially available healthy live insects; Spodoptera litura pheromone is a commercially available conventional slow-release product; eugenol natural antibacterial preservative is a food-grade commercially available product. Other auxiliary materials: diatomaceous earth carrier, 0.22μm filter membrane, and food-grade polyethylene vacuum packaging bags are all commercially available standard products.
[0020] II. Process: Based on the above-mentioned raw materials, this invention proposes a biological control method for diseases, pests, and weeds in the green cultivation of Euryale ferox, which specifically includes the following steps: S1. First, conduct background testing and impurity purification on the planting field and irrigation water source. Then, prepare a composite biocontrol agent with set viable bacteria count and carrier pore size through a multi-step process of slant activation, seed liquid propagation, solid fermentation, and carrier adsorption. Simultaneously prepare biocontrol substrate and propagate the matching natural enemy insect population. S2. Based on the number of viable bacteria in the compound biocontrol agent obtained in step S1, determine the ratio of decomposed organic fertilizer to biocontrol substrate. Mix the pretreated decomposed organic fertilizer, biocontrol substrate and compound biocontrol agent, control the water temperature and tillage parameters, and complete the pretreatment of the rhizosphere microenvironment of the planting field. S3. Transplant the pretreated water chestnut seedlings into the field after the pretreatment in step S2 at the set planting density, and simultaneously apply the compound biocontrol agent and natural enemy insects from step S1 at the set amount to control the water depth and light duration, and maintain the stability of water parameters based on the pretreatment status of the field in step S2. S4. Conduct field monitoring of the growth status of the gorgon fruit seedlings and the occurrence of diseases, pests and weeds in step S3 to obtain basic data on seedling control. Then, add the fermentation by-products from the preparation of the compound biocontrol agent in step S1 to prepare plant-derived inducers, and spray and splash them on the gorgon fruit plants and field water. S5. After the treatment in step S4, the compound biocontrol agent, sex pheromone and natural enemy insects from step S1 are added to the gorgon fruit field according to the set feeding frequency and amount. The dissolved oxygen, pH value and plant population density in the water are controlled. The control frequency is adjusted based on the field monitoring data from step S4. Fresh gorgon fruit is then harvested. S6. First, the plant residues and weeds in the field after harvesting in step S5 are treated by biodegradation. Then, the primary product of gorgon fruit is obtained by cleaning, shelling and low-temperature drying. Based on the pathogen content of fresh gorgon fruit in step S5, a natural antibacterial preservative is added to obtain the final product of gorgon fruit. S7. The field aquaculture wastewater generated from the seedling stage in step S3 to the reproductive growth stage in step S5 is adsorbed by the remaining biocontrol substrate carrier in step S1 and then degraded and purified by the compound biocontrol agent, and recycled for field irrigation. The solid waste generated in the treatment process in step S6 is recycled for field base fertilizer after aerobic composting and fermentation. S8. The final product of the gorgon fruit in step S6 was tested by high performance liquid chromatography and plate count method. Based on the amount of natural antibacterial preservative used in step S6, the packaging container and warehouse conditions were selected for product storage. S9. Record the seedling survival rate during the seedling stage in step S3 and the comprehensive control efficacy of diseases, pests and weeds during the reproductive growth stage in step S5. If the rate is lower than the set threshold, adjust the relevant control process parameters. Based on the test results of step S8, regularly calibrate the dosage and timing of biocontrol materials for each growth stage.
[0021] In one specific implementation, the process of step S1 is as follows: S1.1. Select water chestnut varieties with a resistance level of ≥3 for leaf spot and stem rot. The resistance identification shall be carried out in accordance with NY / T2671-2015. The planting field shall be cleared and leveled, and field residues, weeds and wild snails shall be removed. The flatness error of the field shall be ≤5cm. The irrigation water shall be sand filtered to remove plankton and solid impurities. The pathogen content in the water source shall be ≤10CFU / mL. S1.2. Preparation of a compound biocontrol agent of Bacillus amyloliquefaciens, Bacillus subtilis, and Trichoderma harzianum: Bacillus amyloliquefaciens and Bacillus subtilis were inoculated into LB slant medium, and Trichoderma harzianum was inoculated into PDA slant medium. The mixture was activated at 28℃ for 24 h. Single colonies were picked and inoculated into the corresponding liquid seed culture medium. The mixture was then amplified by shaking at 180 r / min at 28℃ for 24 h to obtain seed liquids. The three seed liquids were inoculated into a solid fermentation medium at a volume ratio of 3:3:4. The solid fermentation medium consisted of 60 parts by weight of wheat bran, 20 parts by weight of soybean meal, 15 parts by weight of corn flour, 3 parts by weight of glucose, 0.5 parts by weight of magnesium sulfate, and 1.5 parts by weight of potassium dihydrogen phosphate, with a water content of 55% and a pH of 6.8-7.0. Fermentation was carried out at 28℃ for 72 h. After adsorption on a diatomaceous earth carrier and drying at 30℃ for 12 h, a total viable count ≥2×10¹ was obtained.0 A composite biocontrol agent with CFU / g and a carrier pore size of 0.2-0.5mm is prepared for use. S1.3. A biocontrol substrate was prepared by mixing 5 parts by weight of rice husk, 3 parts by weight of biochar, and 2 parts by weight of vermiculite. The natural enemy insect populations of Trichogramma wasp, Amblyseius mite, and Viviparus sinensis were simultaneously propagated. Trichogramma wasp propagated using rice moth eggs as hosts at a temperature of 25℃ and a relative humidity of 70%. Amblyseius mite propagated using Tyromitra saprophytic mites as food at a temperature of 26℃ and a relative humidity of 85%. Healthy Viviparus sinensis individuals weighing 10-15g were selected and temporarily raised and acclimatized for 7 days before use.
[0022] In one specific implementation, the process of step S2 is as follows: S2.1. The total viable count of the composite biocontrol agent prepared in step S1 is 2 × 10¹ 0 CFU / g, the pretreated well-rotted organic fertilizer and biocontrol substrate were evenly spread on the planting field at a ratio of 9 parts by weight of well-rotted organic fertilizer and 1 part by weight of biocontrol substrate. The well-rotted organic fertilizer was rapeseed cake fertilizer that had been decomposed at high temperature and aerobically, with an organic matter content of ≥45%. S2.2. Add 0.5 parts by weight of the compound biocontrol agent prepared in step S1 to every 100 parts by weight of the mixed material, control the water temperature of the field at 15-25℃, the tillage depth at 15-20cm, and till twice continuously with an interval of 24 hours between each tillage. After tillage, let it stand for 7 days to complete the pretreatment of the rhizosphere microenvironment of the planting field.
[0023] In one specific implementation, the process of step S3 is as follows: S3.1. Before transplanting, soak the roots of the gorgon fruit seedlings in a 500-fold diluted solution of the compound biocontrol agent prepared in step S1 for 30 minutes. After pretreatment in step S2, control the water depth in the field to 10-15 cm and transplant gorgon fruit seedlings at the 4-leaf and 1-heart stage at a planting density of 1.5 m × 2.0 m. At the same time, apply the compound biocontrol agent prepared in step S1 at a rate of 1.5 kg per mu and release 1500 amblyseius mites per mu. S3.2. Based on the rhizosphere microenvironment of the field, maintain stable water depth and temperature, and monitor the pathogens of stem rot and leaf spot diseases in the field weekly during the growth process, as well as the germination density of duckweed and pondweed.
[0024] In one specific implementation, the process of step S4 is as follows: S4.1. Conduct field monitoring once a week on the seedling stage of the water chestnut field in step S3, record the plant growth status, types and numbers of diseases, pests and weeds, and obtain basic data for seedling control. S4.2. Collect the byproduct of the centrifuged supernatant after solid fermentation during the preparation of the compound biocontrol agent in step S1, filter it through a 0.22μm filter membrane for sterilization, and detect that the content of lipopeptide metabolites is ≥500mg / L. Dilute it with water at a volume ratio of 1:500 to prepare a plant-derived inducer. S4.3. Apply the resistant inducer to the field of water chestnut at a rate of 30L per mu, and simultaneously spray the leaves at a rate of 1L per mu. Repeat the treatment twice at 20-25℃, with an interval of 7 days between each treatment.
[0025] In one specific implementation, the process of step S5 is as follows: S5.1. After the treatment in step S4, control the water depth in the water body to 30-50cm, the dissolved oxygen content to ≥5mg / L, and the pH value to 6.5-7.5. Apply the compound biocontrol agent prepared in step S1 at a rate of 1.0kg per mu and once every 15 days. S5.2. Release Trichogramma wasps in two batches at a total of 10,000 wasps per mu. Hang Spodoptera litura pheromone at a density of 200 pheromones per mu. The active ingredient of the pheromone is cis-9,trans-11-tetradecadiene acetate. The slow-release carrier is a rubber stopper. Hang the pheromone at a height 10cm above the canopy of the Euryale ferox plants. At the same time, release Chinese round snails at a rate of 50kg per mu. S5.3. Based on the field monitoring data from step S4, adjust the control frequency, complete the material application from flowering to seed setting of Euryale ferox, and harvest fresh Euryale ferox fruits.
[0026] In one specific implementation, the process of step S6 is as follows: S6.1. After harvesting fresh gorgon fruit in step S5, control the water depth to 20cm and the water temperature to 20-30℃ in the field. Apply the compound biocontrol agent prepared in step S1 at a rate of 2.0kg per mu. Turn the pond once every 10 days to biodegrade plant residues and weeds in the field. The degradation cycle is 30 days. S6.2. The harvested fresh gorgon fruit is cleaned with water, mechanically dehulled, and dried at a low temperature of 40℃ to obtain the initial product of gorgon fruit; S6.3. Add 0.2 parts by weight of eugenol natural antibacterial preservative to every 100 parts by weight of the initial product of fox nuts, and stir evenly to obtain the final product of fox nuts.
[0027] In one specific implementation, the process of step S7 is as follows: S7.1. Collect field aquaculture wastewater generated from the seedling stage in step S3 to the reproductive growth stage in step S5; S7.2. Pass it into the adsorption tower filled with the remaining biochar carrier from step S1 when preparing the biocontrol substrate. The biochar carrier has a particle size of 2-5 mm. After adsorption treatment, the wastewater is connected to the biological purification tank. Add 0.5 parts by mass of the composite biocontrol agent prepared in step S1 per cubic meter of water. Control the hydraulic retention time to 72 h, the dissolved oxygen content of aeration ≥ 4 mg / L, and the chemical oxygen demand of the effluent after degradation and purification ≤ 50 mg / L and ammonia nitrogen ≤ 5 mg / L, and then recycle it for field irrigation. S7.3. Collect the solid waste generated during the treatment of step S6, such as the husks and plant residues after degradation, and compost them aerobically for 30 days. After decomposition, reuse them as base fertilizer in the field.
[0028] In one specific implementation, the process of step S8 is as follows: S8.1. The final product of gorgon fruit obtained in step S6 is tested. Pesticide residues are detected by high performance liquid chromatography, and the total number of pathogens is detected by plate count method. The pesticide residues in the final product of gorgon fruit are controlled to be undetectable, the total number of pathogens is ≤10 CFU / g, and the moisture content is ≤13%. S8.2. Based on the amount of eugenol natural antibacterial preservative added in step S6, seal the qualified product into food-grade polyethylene vacuum packaging bags, each bag containing 5 kg net weight, and store in a cool and ventilated warehouse with the temperature controlled at 0-4℃ and the relative humidity controlled at 45-55%, avoiding light exposure.
[0029] In one specific implementation, the process of step S9 is as follows: S9.1. Record the seedling survival rate during the seedling stage in step S3 and the comprehensive control efficacy of pests, diseases and weeds during the reproductive growth stage in step S5. When the seedling survival rate during the seedling stage is less than 93%, adjust the dosage of compound biocontrol agent in step S2 to 0.7 parts by weight per 100 parts by weight of mixed material, and increase the application rate of compound biocontrol agent in step S3 to 2.0 kg per mu. S9.2. When the comprehensive control efficacy of pests, diseases and weeds during the reproductive growth period is less than 82%, the frequency of supplementary application of the compound biocontrol agent in step S5 is adjusted to once every 10 days. At the same time, 50% of the initial total amount applied in step S5.2 is supplemented with the release of Trichogramma wasps and Atlas seimitus. S9.3. Based on the product testing results of step S8, recalibrate the dosage of the inducer in step S4 and the biocontrol material delivery parameters in step S5 after every 5 batches of production.
[0030] Example 1: This example is prepared according to the above process, and the core parameters are as follows: The seed liquid volume ratio of the compound biocontrol agent is: Bacillus amyloliquefaciens: Bacillus subtilis: Trichoderma harzianum = 3:3:4; Solid fermentation medium formula: 60 parts by weight of wheat bran, 20 parts by weight of soybean meal, 15 parts by weight of corn flour, 3 parts by weight of glucose, 0.5 parts by weight of magnesium sulfate, and 1.5 parts by weight of potassium dihydrogen phosphate; Biocontrol substrate formulation: 5 parts by weight of rice husk, 3 parts by weight of biochar, and 2 parts by weight of vermiculite; Step S2: Dosage of compound biocontrol agent: Add 0.5 parts by weight per 100 parts by weight of the mixture; Step S3: Application rate of compound biocontrol agent: 1.5 kg per mu; release rate of amblyseius mites: 1500 per mu. Step S4: Plant-derived resistance inducer treatment: Apply 30L per acre by spraying or foliar spraying, repeat twice, with an interval of 7 days. Step S5: Application of compound biocontrol agent: 1.0 kg per mu, once every 15 days; total number of Trichogramma wasps released per mu: 10,000; pheromone hanging density: 200 per mu; and amount of Chinese round snails released per mu: 50 kg. Step S6: Dosage of eugenol natural antibacterial preservative: Add 0.2 parts by weight per 100 parts by weight of the initial product of Euryale ferox; The remaining process parameters are completely consistent with the specific implementation method described above.
[0031] Example 2: In this example, the dosage of compound biocontrol agent in step S2 is adjusted to 0.4 parts by weight per 100 parts by weight of the mixture, the application rate of compound biocontrol agent in step S3 is adjusted to 1.2 kg per mu, and the supplementary application rate of compound biocontrol agent in step S5 is adjusted to 0.8 kg per mu. The remaining formula and process parameters are completely consistent with those in Example 1.
[0032] Example 3: In this example, the dosage of compound biocontrol agent in step S2 is adjusted to 0.6 parts by weight per 100 parts by weight of mixed material, the application rate of compound biocontrol agent in step S3 is adjusted to 1.8 kg per mu, and the supplementary application rate of compound biocontrol agent in step S5 is adjusted to 1.2 kg per mu. The remaining formula and process parameters are completely consistent with those in Example 1.
[0033] The core formulation parameters of the examples are summarized in Table 1: Table 1: Core Formulation Parameters of Examples
[0034] Comparative Example 1: In this comparative example, the compound biocontrol agent only used Bacillus amyloliquefaciens, without the addition of Bacillus subtilis and Trichoderma harzianum. The rest of the formulation and process parameters were completely consistent with those of Example 1.
[0035] Comparative Example 2: In this comparative example, no natural enemy insects such as Trichogramma, Amblyseius, and Viviparus sinensis were used, and no Spodoptera litura pheromone was used. The rest of the formula and process parameters were completely consistent with those of Example 1.
[0036] Comparative Example 3: In this comparative example, the amount of compound biocontrol agent in step S2 was adjusted to 1.0 part by mass per 100 parts by mass of the mixture. The remaining formula and process parameters were completely consistent with those in Example 1.
[0037] The adjustment of the scaling parameters is shown in Table 2: Table 2: Adjustment Table for Proportional Parameters
[0038] III. Performance Testing: The following performance tests were conducted on the *Euryale ferox* planting systems prepared in Examples 1-3 and Comparative Examples 1-3. All tests were performed in triplicate, and the average value was taken: a. Seedling survival rate: The number of surviving seedlings 30 days after transplanting was counted, and the seedling survival rate was calculated using the following formula: Survival rate (%) = Number of surviving plants / Total number of plants planted × 100%; b. Integrated pest and weed control efficacy: Referring to NY / T2671-2015 "Field Efficacy Test Guidelines for Crop Disease Control", NY / T1464.7-2007 "Field Efficacy Test Guidelines for Pesticides Part 7: Insecticides for the Control of Lepidoptera Pests in Vegetables", and NY / T1464.48-2012 "Field Efficacy Test Guidelines for Pesticides Part 48: Herbicides for the Control of Weeds in Aquatic Vegetable Fields", the disease control efficacy against stem rot and leaf spot, the population reduction rate of beet armyworm and golden apple snail, and the weed control efficacy were tested respectively, and the integrated control efficacy was calculated according to the weights. c. Final product quality of Gorgon fruit: Refer to NY / T1042-2017 "Green Food Nuts" to test for pesticide residues, total number of pathogenic bacteria, and moisture content; d. Quality of reused irrigation water: Refer to GB5084-2021 "Standards for Irrigation Water Quality in Farmland" to test the chemical oxygen demand and ammonia nitrogen content of the purified reused wastewater.
[0039] The performance test results are shown in Table 3: Table 3: Performance Test Results of Examples and Comparative Examples
[0040] IV. Analysis Conclusion The test data in Table 1-3 show that: All indicators of Examples 1-3 of this invention have achieved the expected results. Among them, Example 1 is the optimal solution, with a seedling survival rate of 96.2%, an integrated pest and weed control effect of 88.6%, and all indicators of the final product of Euryale ferox meeting the requirements of NY / T1042-2017 standard. The quality of the reused wastewater meets the GB5084-2021 farmland irrigation water quality standard, which verifies the stability and reliability of the technical solution of this invention.
[0041] Comparative Example 1 used a single Bacillus amyloliquefaciens, which did not achieve the synergistic effect of the three strains. The disease control efficacy of stem rot was significantly reduced, and the comprehensive control efficacy against diseases, pests and weeds was only 70.5%, which was 18.1 percentage points lower than that of Example 1. At the same time, the seedling survival rate dropped to 90.3%, which verified the synergistic effect of the three strains of the compound biocontrol agent of the present invention, which is the core of achieving efficient disease control.
[0042] In Comparative Example 2, without the application of natural enemy insects and sex pheromones, the population reduction rate of Spodoptera litura was only 65.2%, a decrease of 27.3 percentage points compared to Example 1. The comprehensive control efficacy against diseases, pests, and weeds decreased to 72.3%, verifying the necessity of the synergistic control system of the fungal agent and natural enemy insects of this invention. The combination of the two can achieve all-dimensional control of diseases, pests, and weeds.
[0043] In Comparative Example 3, the dosage of the compound biocontrol agent exceeded the parameter range set in this invention, resulting in a seedling survival rate of 88.5% and a comprehensive control efficacy of only 76.8% for diseases, pests, and weeds. This indicates that excessive addition of the agent will disrupt the microecological balance of the rhizosphere in the field and negatively affect the normal growth of the gorgon fruit seedlings. This verifies the scientific validity and rationality of the agent dosage parameter range set in this invention.
[0044] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A method for preventing and controlling green planting pests, diseases and weeds of Euryale ferox, characterized in that, Includes the following steps: S1. First, conduct background testing and impurity purification on the planting field and irrigation water source. Then, prepare a composite biocontrol agent with set viable bacteria count and carrier pore size through a multi-step process of slant activation, seed liquid propagation, solid fermentation, and carrier adsorption. Simultaneously prepare biocontrol substrate and propagate the matching natural enemy insect population. S2. Based on the number of viable bacteria in the compound biocontrol agent obtained in step S1, determine the ratio of decomposed organic fertilizer to biocontrol substrate. Mix the pretreated decomposed organic fertilizer, biocontrol substrate and compound biocontrol agent, control the water temperature and tillage parameters, and complete the pretreatment of the rhizosphere microenvironment of the planting field. S3. Transplant the pretreated water chestnut seedlings into the field after the pretreatment in step S2 at the set planting density, and simultaneously apply the compound biocontrol agent and natural enemy insects from step S1 at the set amount to control the water depth and light duration, and maintain the stability of water parameters based on the pretreatment status of the field in step S2. S4. Conduct field monitoring of the growth status of the gorgon fruit seedlings and the occurrence of diseases, pests and weeds in step S3 to obtain basic data on seedling control. Then, add the fermentation by-products from the preparation of the compound biocontrol agent in step S1 to prepare plant-derived inducers, and spray and splash them on the gorgon fruit plants and field water. S5. After the treatment in step S4, the compound biocontrol agent, sex pheromone and natural enemy insects from step S1 are added to the gorgon fruit field according to the set feeding frequency and amount. The dissolved oxygen, pH value and plant population density in the water are controlled. The control frequency is adjusted based on the field monitoring data from step S4. Fresh gorgon fruit is then harvested. S6. First, the plant residues and weeds in the field after harvesting in step S5 are treated by biodegradation. Then, the primary product of gorgon fruit is obtained by cleaning, shelling and low-temperature drying. Based on the pathogen content of fresh gorgon fruit in step S5, a natural antibacterial preservative is added to obtain the final product of gorgon fruit. S7. The field aquaculture wastewater generated from the seedling stage in step S3 to the reproductive growth stage in step S5 is adsorbed by the remaining biocontrol substrate carrier in step S1 and then degraded and purified by the compound biocontrol agent, and recycled for field irrigation. The solid waste generated in the treatment process in step S6 is recycled for field base fertilizer after aerobic composting and fermentation. S8. The final product of the gorgon fruit in step S6 was tested by high performance liquid chromatography and plate count method. Based on the amount of natural antibacterial preservative used in step S6, the packaging container and warehouse conditions were selected for product storage. S9. Record the seedling survival rate during the seedling stage in step S3 and the comprehensive control efficacy of diseases, pests and weeds during the reproductive growth stage in step S5. If the rate is lower than the set threshold, adjust the relevant control process parameters. Based on the test results of step S8, regularly calibrate the dosage and timing of biocontrol materials for each growth stage.
2. The method according to claim 1, wherein the method is characterized by, The implementation process of step S1 is as follows: S1.
1. Select water chestnut varieties with a resistance level of ≥3 for leaf spot and stem rot, clear and level the planting field, remove field residues, weeds and wild snails, and ensure the flatness error of the field is ≤5cm. Filter the irrigation water with sand to remove plankton and solid impurities, and test the pathogen content in the water source to ≤10CFU / mL. S1.
2. Preparation of a compound biocontrol agent of Bacillus amyloliquefaciens, Bacillus subtilis, and Trichoderma harzianum: Bacillus amyloliquefaciens and Bacillus subtilis were inoculated into LB slant medium, and Trichoderma harzianum was inoculated into PDA slant medium. The mixture was activated at 28℃ for 24 h. Single colonies were picked and inoculated into the corresponding liquid seed culture medium. The mixture was then amplified by shaking at 180 r / min at 28℃ for 24 h to obtain seed liquids. The three seed liquids were inoculated into a solid fermentation medium at a volume ratio of 3:3:
4. The solid fermentation medium consisted of 60 parts by weight of wheat bran, 20 parts by weight of soybean meal, 15 parts by weight of corn flour, 3 parts by weight of glucose, 0.5 parts by weight of magnesium sulfate, and 1.5 parts by weight of potassium dihydrogen phosphate, with a water content of 55% and a pH of 6.8-7.
0. Fermentation was carried out at 28℃ for 72 h. After adsorption on a diatomaceous earth carrier and drying at 30℃ for 12 h, a total viable count ≥2×10¹ was obtained. 0 A composite biocontrol agent with CFU / g and a carrier pore size of 0.2-0.5mm is prepared for use. S1.
3. A biocontrol substrate was prepared by mixing 5 parts by weight of rice husk, 3 parts by weight of biochar, and 2 parts by weight of vermiculite. The natural enemy insect populations of Trichogramma wasp, Amblyseius mite, and Viviparus sinensis were simultaneously propagated. Trichogramma wasp propagated using rice moth eggs as hosts at a temperature of 25℃ and a relative humidity of 70%. Amblyseius mite propagated using Tyromitra saprophytic mites as food at a temperature of 26℃ and a relative humidity of 85%. Healthy Viviparus sinensis individuals weighing 10-15g were selected and temporarily raised and acclimatized for 7 days before use.
3. The method according to claim 2, wherein the method is characterized by, The implementation process of step S2 is as follows: S2.
1. Total viable bacteria number of the composite biocontrol agent prepared based on step S1 2 x 101 0 CFU / g, the pretreated mature organic fertilizer and the biocontrol substrate are uniformly applied to the planting field in a ratio of 9 parts by mass of mature organic fertilizer to 1 part by mass of biocontrol substrate, the mature organic fertilizer is rapeseed cake fertilizer matured by high-temperature aerobic composting, and the organic matter content is ≥ 45%. S2.
2. Add 0.5 parts by weight of the compound biocontrol agent prepared in step S1 to every 100 parts by weight of the mixed material, control the water temperature of the field at 15-25℃, the tillage depth at 15-20cm, and till twice continuously with an interval of 24 hours between each tillage. After tillage, let it stand for 7 days to complete the pretreatment of the rhizosphere microenvironment of the planting field.
4. The method according to claim 3, wherein the method is characterized by, The implementation process of step S3 is as follows: S3.
1. Before transplanting, soak the roots of the gorgon fruit seedlings in a 500-fold diluted solution of the compound biocontrol agent prepared in step S1 for 30 minutes. After pretreatment in step S2, control the water depth in the field to 10-15 cm and transplant gorgon fruit seedlings at the 4-leaf and 1-heart stage at a planting density of 1.5 m × 2.0 m. At the same time, apply the compound biocontrol agent prepared in step S1 at a rate of 1.5 kg per mu and release 1500 amblyseius mites per mu. S3.
2. Based on the rhizosphere microenvironment of the field, maintain stable water depth and temperature, and monitor the pathogens of stem rot and leaf spot diseases in the field weekly during the growth process, as well as the germination density of duckweed and pondweed.
5. The method according to claim 4, wherein the method is characterized by, The implementation process of step S4 is as follows: S4.
1. Conduct field monitoring once a week on the seedling stage of the water chestnut field in step S3, record the plant growth status, types and numbers of diseases, pests and weeds, and obtain basic data for seedling control. S4.
2. Collect the byproduct of the centrifuged supernatant after solid fermentation during the preparation of the compound biocontrol agent in step S1, filter it through a 0.22μm filter membrane for sterilization, and detect that the content of lipopeptide metabolites is ≥500mg / L. Dilute it with water at a volume ratio of 1:500 to prepare a plant-derived inducer. S4.
3. Apply the resistant inducer to the field of water chestnut at a rate of 30L per mu, and simultaneously spray the leaves at a rate of 1L per mu. Repeat the treatment twice at 20-25℃, with an interval of 7 days between each treatment.
6. The method according to claim 5, wherein the method is characterized by, The implementation process of step S5 is as follows: S5.
1. After the treatment in step S4, control the water depth in the water body to 30-50cm, the dissolved oxygen content to ≥5mg / L, and the pH value to 6.5-7.
5. Apply the compound biocontrol agent prepared in step S1 at a rate of 1.0kg per mu and once every 15 days. S5.
2. Release Trichogramma wasps in two batches at a total of 10,000 wasps per mu. Hang Spodoptera litura pheromone at a density of 200 pheromones per mu. The active ingredient of the pheromone is cis-9,trans-11-tetradecadiene acetate. The slow-release carrier is a rubber stopper. Hang the pheromone at a height 10cm above the canopy of the Euryale ferox plants. At the same time, release Chinese round snails at a rate of 50kg per mu. S5.
3. Based on the field monitoring data from step S4, adjust the control frequency, complete the material application from flowering to seed setting of Euryale ferox, and harvest fresh Euryale ferox fruits.
7. A method for biological control of pests, diseases, and weeds in green cultivation of Euryale ferox according to claim 6, characterized in that, The implementation process of step S6 is as follows: S6.
1. After harvesting fresh gorgon fruit in step S5, control the water depth to 20cm and the water temperature to 20-30℃ in the field. Apply the compound biocontrol agent prepared in step S1 at a rate of 2.0kg per mu. Turn the pond once every 10 days to biodegrade plant residues and weeds in the field. The degradation cycle is 30 days. S6.
2. The harvested fresh gorgon fruit is cleaned with water, mechanically dehulled, and dried at a low temperature of 40℃ to obtain the initial product of gorgon fruit; S6.
3. Add 0.2 parts by weight of eugenol natural antibacterial preservative to every 100 parts by weight of the initial product of fox nuts, and stir evenly to obtain the final product of fox nuts.
8. A method for biological control of pests, diseases, and weeds in green cultivation of water chestnut according to claim 7, characterized in that, The implementation process of step S7 is as follows: S7.
1. Collect field aquaculture wastewater generated from the seedling stage in step S3 to the reproductive growth stage in step S5; S7.
2. Pass it into the adsorption tower filled with the remaining biochar carrier from step S1 when preparing the biocontrol substrate. The biochar carrier has a particle size of 2-5 mm. After adsorption treatment, the wastewater is connected to the biological purification tank. Add 0.5 parts by mass of the composite biocontrol agent prepared in step S1 per cubic meter of water. Control the hydraulic retention time to 72 h, the dissolved oxygen content of aeration ≥ 4 mg / L, and the chemical oxygen demand of the effluent after degradation and purification ≤ 50 mg / L and ammonia nitrogen ≤ 5 mg / L, and then recycle it for field irrigation. S7.
3. Collect the solid waste generated during the treatment of step S6, such as the husks and plant residues after degradation, and compost them aerobically for 30 days. After decomposition, reuse them as base fertilizer in the field.
9. The method according to claim 8, wherein the method is characterized by, The implementation process of step S8 is as follows: S8.
1. The final product of gorgon fruit obtained in step S6 is tested. Pesticide residues are detected by high performance liquid chromatography, and the total number of pathogens is detected by plate count method. The pesticide residues in the final product of gorgon fruit are controlled to be undetectable, the total number of pathogens is ≤10 CFU / g, and the moisture content is ≤13%. S8.
2. Based on the amount of eugenol natural antibacterial preservative added in step S6, seal the qualified product into food-grade polyethylene vacuum packaging bags, each bag containing 5 kg net weight, and store in a cool and ventilated warehouse with the temperature controlled at 0-4℃ and the relative humidity controlled at 45-55%, avoiding light exposure.
10. The method according to claim 9, wherein the method is characterized by, The implementation process of step S9 is as follows: S9.
1. Record the seedling survival rate during the seedling stage in step S3 and the comprehensive control efficacy of pests, diseases and weeds during the reproductive growth stage in step S5. When the seedling survival rate during the seedling stage is less than 93%, adjust the dosage of compound biocontrol agent in step S2 to 0.7 parts by weight per 100 parts by weight of mixed material, and increase the application rate of compound biocontrol agent in step S3 to 2.0 kg per mu. S9.
2. When the comprehensive control efficacy of pests, diseases and weeds during the reproductive growth period is less than 82%, the frequency of supplementary application of the compound biocontrol agent in step S5 is adjusted to once every 10 days. At the same time, 50% of the initial total amount applied in step S5.2 is supplemented with the release of Trichogramma wasps and Atlas seimitus. S9.
3. Based on the product testing results of step S8, recalibrate the dosage of the inducer in step S4 and the biocontrol material delivery parameters in step S5 after every 5 batches of production.