A lysozyme-nano biomimetic system based on cell wall signal regulation and application thereof in crop global quality improvement and synergism

Abstract

This invention belongs to the field of agricultural biotechnology and discloses a lysozyme-nanomobionic system based on cell wall signal regulation and its application in improving the quality and efficiency of crops across the entire process. The system consists of a core engine, a biomimetic delivery system, and an enhancement module. It utilizes plant exosomes to simulate vesicle delivery, employing lysozyme to hydrolyze the cell walls of pathogens, releasing oligosaccharide signaling molecules, activating plant WAK receptors, and simultaneously initiating disease resistance, quality improvement, and yield enhancement pathways. Intelligent pH / ROS-responsive gating precisely releases components, while the nano-silicon photosynthetic engine enhances light efficiency, resulting in increased crop disease resistance, improved quality, and increased yield, while also promoting the green and low-carbon transformation of agriculture.

Description

Technical Field

[0001] This invention belongs to the field of agricultural biotechnology, and more specifically, it relates to a lysozyme-nanobionic system based on cell wall signal regulation and its application in improving the quality and efficiency of crops across the entire crop range. Background Technology

[0002] In the field of agricultural biotechnology, the current bottleneck in increasing global crop yields is the average annual increase of less than 1%, and the coordinated achievement of improved crop quality, enhanced disease resistance, and increased yield is difficult, becoming a key issue restricting sustainable agricultural development. Lysozyme, as a biological agent with antibacterial potential, faces numerous limitations in its application, including differences in permeability across crop cell walls (e.g., structural differences between monocotyledonous and dicotyledonous plants), the inability to simultaneously activate multiple physiological pathways in plants, and environmental sensitivity (e.g., inactivation due to pH changes and ultraviolet radiation). Existing technologies, such as lysozyme-chitosan leaf masks, are only suitable for fruit and vegetable crops; methods using engineered bacteria to secrete lysozyme suffer from unstable colonization in the field; and the combination of lysozyme and growth hormones can easily induce excessive vegetative growth, leading to yield reduction. These solutions fail to effectively address the challenge of differences in permeability across crop cell walls, neglect the value of lysozyme hydrolysates as signaling molecules regulating plant physiological pathways, and lack environmentally adaptive intelligent release designs, resulting in a narrow application range and unstable effects.

[0003] Therefore, this invention provides a lysozyme-nanobionic system based on cell wall signal regulation and its application in improving the quality and efficiency of crops across the entire crop range. Summary of the Invention

[0004] In view of the above-mentioned problems of existing technologies, the purpose of this invention is to provide a lysozyme-nano-bionic system based on cell wall signal regulation and its application in improving the quality and efficiency of crops across the entire field. By using a bionic delivery carrier that can be adapted to various crops, precisely activating crop disease resistance and quality improvement signal pathways, intelligently responding to release core components and enhancing photosynthesis, it can achieve overall quality and efficiency improvement and promote the green and low-carbon transformation of agriculture.

[0005] The objective of this invention can be achieved through the following technical solutions: A lysozyme-nano-bionic system based on cell wall signal regulation, wherein the lysozyme-nano-bionic system includes a core engine, a bionic delivery system, and an enhancement module; The core engine comprises a broad-spectrum lysozyme and a cell wall signal amplifier. The broad-spectrum lysozyme accounts for 0.8%-2.2% of the lysozyme-nanomobionic system by weight, and the cell wall signal amplifier accounts for 0.05%-0.15% of the lysozyme-nanomobionic system by weight. The biomimetic delivery system comprises plant exosome-mimicking vesicles and a pH / ROS dual-response gating system, wherein the plant exosome-mimicking vesicles account for 1.5%-4% by weight of the lysozyme-nanomomimetic system. The enhancement module includes a nano-silicon photosynthetic engine and a stress-resistance factor. The nano-silicon photosynthetic engine accounts for 0.001%-0.005% of the lysozyme-nano-bionic system by weight, and the stress-resistance factor accounts for 0.3%-0.9% of the lysozyme-nano-bionic system by weight. The remaining components of the lysozyme-nanobionic system are auxiliary materials.

[0006] As a further preferred technical solution of the present invention, the broad-spectrum lysozyme is a chimeric lysozyme, which has dual active centers of plant chitinase and glucanase, and can hydrolyze the chitin and glucan components of the cell wall of pathogens. The dual active centers are obtained through gene chimerism technology. The cell wall signal amplifier is an oligogalacturonic acid fragment with a degree of polymerization (DP) of 6-8.

[0007] As a further preferred technical solution of the present invention, the surface of the plant exosome mimic vesicles is modified with sodium lignin sulfonate or pectin lyase synergist, and the plant exosome mimic vesicles are composed of soybean phospholipids and sterols in a weight ratio of 7:3, and the particle size is 80±10nm. The pH / ROS dual-response gating is composed of a polydopamine-hyaluronic acid composite membrane, and the pH / ROS dual-response gating response conditions are as follows: The polydopamine layer dissolves at pH values ​​below 5.5; Under conditions of reactive oxygen species (ROS) bursts, hyaluronic acid chains break.

[0008] As a further preferred technical solution of the present invention, the nano-silicon photosynthetic engine is a chloroplast-targeted CdSe / ZnS quantum dot, and the stress resistance factor is a proline-betaine eutectic.

[0009] A method for preparing a lysozyme-nanomobionic system, comprising the following steps: S1: Preparation of plant exosome-simulated vesicles: Soybean phospholipids and phytosterols were dissolved in an organic solvent in a certain proportion, and blank vesicles were prepared by thin-film evaporation-hydration method; S2: Loading active ingredients: Broad-spectrum lysozyme, cell wall signal amplifier, nano-silicon photosynthetic engine and stress resistance factor are dissolved or dispersed in buffer solution, and encapsulated in the blank vesicles obtained in step S1 by active drug loading or ultrasonic emulsification to obtain drug-loaded vesicles; S3: Constructing a response-gated membrane: Under weakly alkaline conditions, the drug-loaded vesicles obtained in step S2 are co-incubated with dopamine monomers to polymerize dopamine on the vesicle surface to form a polydopamine layer; subsequently, hyaluronic acid is modified onto the polydopamine layer by electrostatic adsorption or covalent crosslinking to form a polydopamine-hyaluronic acid composite membrane.

[0010] As a further preferred technical solution of the present invention, in step S1, the organic solvent is a mixed solvent of chloroform and methanol, and the temperature of the thin film evaporation is 35-45℃.

[0011] An application of a lysozyme-nanomobionic system in regulating plant physiological pathways, wherein the application of the lysozyme-nanomobionic system in regulating plant physiological pathways includes: Oligosaccharide fragments released by lysozyme from the hydrolysis of pathogen cell walls act as signaling molecules, simultaneously activating plant disease resistance pathways, quality improvement pathways, and yield increase pathways by binding to the plant cell wall-associated kinase (WAK) receptor.

[0012] As a further preferred technical solution of the present invention, the synchronous activation includes: Activate the MAPK cascade antiviral pathway; Activation of MYB transcription factor to induce the synthesis of secondary metabolites such as anthocyanins; Promote EXPANSIN gene expression to enhance cell wall relaxation and nutrient transport efficiency.

[0013] An application of a lysozyme-nanomobionic system in improving crop quality and efficiency, based on the aforementioned lysozyme-nanomobionic system, with different formulations and modification strategies adapted according to crop type, including: For gramineous crops, the suitable formulation is water-dispersible granules, and the surface of the biomimetic delivery body is modified with sodium lignosulfonate. For Solanaceae crops, the suitable formulation is nanoemulsion, with pectin lyase added as a synergist; For fruit trees, the appropriate formulation is a gel injected into the trunk, and the osmotic pressure of the system is adjusted to -0.8 MPa; For tuber crops, the suitable formulation is seed potato coated microspheres, which are loaded with gibberellin A4 in the system.

[0014] As a further preferred technical solution of the present invention, the crops include rice, tomatoes, citrus or potatoes. After applying the lysozyme-nano-bionic system, crop yield can be increased, crop quality indicators can be improved, and the loss rate caused by biotic or abiotic stress can be reduced.

[0015] As described above, the lysozyme-nanomomastic system based on cell wall signal regulation provided by the present invention and its application in improving the quality and efficiency of crops across the entire crop range have the following beneficial effects: 1. This invention utilizes a lysozyme-nanomomorphic system based on cell wall signal regulation and its application in improving the quality and efficiency of crops across the entire crop range. Compared with existing technologies, this invention uses a unique plant exosome-like vesicle as a biomimetic delivery vehicle. Its 80nm size and surface characteristics that can be modified with sodium lignin sulfonate or pectin lyase can efficiently simulate the biological endogenous transport mechanism. By using endocytosis to cross cell walls of different structures, the permeability efficiency is improved compared with traditional carriers. This core design fundamentally solves the problem of unresolved permeability differences across crop cell walls, making the same technology platform adaptable to six major crop families, including rice (grass family), tomato (solanaceae family), citrus (fruit tree), and potato (tuberous crop), achieving full-range adaptability.

[0016] 2. This invention utilizes the aforementioned lysozyme-nanomometic system based on cell wall signal regulation and its application in improving the quality and efficiency of crops across the entire process. Compared with existing technologies, this invention utilizes oligosaccharide fragments, such as oligogalacturonic acid, produced by lysozyme hydrolyzing the cell walls of pathogens as signaling molecules to specifically activate plant cell wall-associated kinase (WAK) receptors, simultaneously initiating three physiological pathways: ① Activating the MAPK cascade disease resistance pathway, significantly enhancing the crop's resistance to biological stresses such as rice blast and bacterial wilt, reducing disease loss rates by 40%-65%; ② Activating MYB transcription factors, driving the synthesis of secondary metabolites such as anthocyanins, lycopene, and vitamin C, improving fruit and vegetable quality indicators by 25%-30% and grain protein content by 15%-18%; ③ Promoting EXPANSIN gene expression, relaxing cell walls, promoting cell expansion and nutrient transport, laying the foundation for yield improvement.

[0017] 3. This invention utilizes the aforementioned lysozyme-nanomomastic system based on cell wall signal regulation and its application in improving the quality and efficiency of crops across the entire crop range. Compared with existing technologies, this invention designs a pH / ROS dual-response gating system: a polydopamine-hyaluronic acid composite membrane. This system remains stable in healthy crop tissues, i.e., under normal pH conditions, reducing the loss of effective components. Once it reaches the disease-infected area where the pH is <5.5 or the crop encounters stress and produces a large amount of reactive oxygen species, resulting in a ROS burst, the gating system will respond rapidly and precisely release the core engine and stress-resistant factors. This on-demand supply mode not only improves the utilization rate of effective components but also enhances the ability to alleviate abiotic stresses such as drought and salinity, realizing intelligent management that adapts to the environment.

[0018] 4. This invention utilizes the aforementioned lysozyme-nanomomastic system based on cell wall signal regulation and its application in improving the quality and efficiency of crops across the entire crop range. Compared with existing technologies, this invention employs a nano-silicon photosynthetic engine: chloroplast-targeted CdSe / ZnS quantum dots, which can convert ineffective ultraviolet light into blue and red light that can be efficiently utilized by chloroplasts, thereby improving photosynthetic efficiency. The increased photosynthetic products provide sufficient material and energy sources for the synthesis of stress-resistant factors: amino acid-betaine cocrystals and the accumulation of secondary metabolites, forming a virtuous cycle of increasing supply and reducing consumption (and improving quality), ensuring the achievement of the goal of improving quality and efficiency across the entire crop range from the perspective of energy supply.

[0019] 5. This invention utilizes a lysozyme-nanomomastic system based on cell wall signal regulation and its application in improving crop quality and efficiency. Compared with existing technologies, this invention significantly reduces the input of traditional chemical pesticides and fertilizers. Its formulation production uses green electricity throughout, and the packaging material is derived from crop straw, resulting in a certified reduction of 3.2 tCO2e / ha in carbon footprint. While ensuring high yield and quality in agriculture, it promotes the green and low-carbon transformation of agriculture, possessing long-term social and environmental value.

[0020] Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0022] Figure 1 This is a schematic diagram of the overall structure of a lysozyme-nano-bionic system based on cell wall signal regulation and its application in improving the quality and efficiency of crops. Figure 2 This invention relates to a lysozyme-nanomomastic system based on cell wall signal regulation and its application in improving crop quality and efficiency, illustrating the mechanism of its pH / ROS dual-response gating. Figure 3 This invention relates to a lysozyme-nanobionic system based on cell wall signal regulation and its application in improving the quality and efficiency of crops across the entire crop growing area. Figure 4 The flowchart illustrates the preparation process of a lysozyme-nanomobionic system based on cell wall signal regulation, and its application in improving the quality and efficiency of crops. Detailed Implementation

[0023] The following specific embodiments illustrate the implementation of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification.

[0024] It should be understood that the structures, proportions, sizes, etc., depicted in the accompanying drawings of this specification are merely for illustrative purposes to aid those skilled in the art and are not intended to limit the scope of the invention. Therefore, they have no substantial technical significance. Any modifications to the structure, changes in proportions, or adjustments to size, without affecting the effectiveness and purpose of the invention, should still fall within the scope of the technical content disclosed in this invention. Furthermore, the terms such as "upper," "lower," "left," "right," "middle," and "one" used in this specification are merely for clarity and are not intended to limit the scope of the invention. Changes or adjustments to their relative relationships, without substantially altering the technical content, should also be considered within the scope of the invention. Specific structures can be described with reference to the accompanying drawings of the patent application.

[0025] This invention provides a lysozyme-nanomomastic system based on cell wall signal regulation and its application in improving the quality and efficiency of crops across the entire crop growing process. Please refer to [link / reference]. Figures 1 to 4 As shown, a lysozyme-nano-bionic system based on cell wall signal regulation is disclosed. The lysozyme-nano-bionic system includes a core engine, a bionic delivery system, and an enhancement module. The core engine comprises a broad-spectrum lysozyme and a cell wall signal amplifier. The broad-spectrum lysozyme accounts for 0.8%-2.2% of the lysozyme-nanomobionic system by weight, and the cell wall signal amplifier accounts for 0.05%-0.15% of the lysozyme-nanomobionic system by weight. The biomimetic delivery system comprises plant exosome-mimicking vesicles and a pH / ROS dual-response gating system, wherein the plant exosome-mimicking vesicles account for 1.5%-4% by weight of the lysozyme-nanomomimetic system. The enhancement module includes a nano-silicon photosynthetic engine and a stress-resistance factor. The nano-silicon photosynthetic engine accounts for 0.001%-0.005% of the lysozyme-nano-bionic system by weight, and the stress-resistance factor accounts for 0.3%-0.9% of the lysozyme-nano-bionic system by weight. The remaining components of the lysozyme-nanobionic system are auxiliary materials.

[0026] The broad-spectrum lysozyme is a chimeric lysozyme, which has dual active centers of plant chitinase and glucanase, and can hydrolyze the chitin and glucan components of the cell wall of pathogens. The dual active centers are obtained through gene chimerism technology. The cell wall signal amplifier is an oligogalacturonic acid fragment with a degree of polymerization (DP) of 6-8.

[0027] The plant exosome-like vesicles are surface-modified with sodium lignin sulfonate or pectin lyase synergist, and the plant exosome-like vesicles are composed of soybean phospholipids and sterols in a weight ratio of 7:3, with a particle size of 80±10nm. The pH / ROS dual-response gating is composed of a polydopamine-hyaluronic acid composite membrane, and the pH / ROS dual-response gating response conditions are as follows: The polydopamine layer dissolves at pH values ​​below 5.5; Under conditions of reactive oxygen species (ROS) bursts, hyaluronic acid chains break.

[0028] As a further preferred technical solution of the present invention, the nano-silicon photosynthetic engine is a chloroplast-targeted CdSe / ZnS quantum dot, and the stress resistance factor is a proline-betaine eutectic.

[0029] A method for preparing a lysozyme-nanomobionic system, comprising the following steps: S1: Preparation of plant exosome-simulated vesicles: Soybean phospholipids and phytosterols were dissolved in an organic solvent in a certain proportion, and blank vesicles were prepared by thin-film evaporation-hydration method; S2: Loading active ingredients: Broad-spectrum lysozyme, cell wall signal amplifier, nano-silicon photosynthetic engine and stress resistance factor are dissolved or dispersed in buffer solution, and encapsulated in the blank vesicles obtained in step S1 by active drug loading or ultrasonic emulsification to obtain drug-loaded vesicles; S3: Constructing a response-gated membrane: Under weakly alkaline conditions, the drug-loaded vesicles obtained in step S2 are co-incubated with dopamine monomers to polymerize dopamine on the vesicle surface to form a polydopamine layer; subsequently, hyaluronic acid is modified onto the polydopamine layer by electrostatic adsorption or covalent crosslinking to form a polydopamine-hyaluronic acid composite membrane.

[0030] In step S1, the organic solvent is a mixture of chloroform and methanol, and the film evaporation temperature is 35-45°C.

[0031] An application of a lysozyme-nanomobionic system in regulating plant physiological pathways, wherein the application of the lysozyme-nanomobionic system in regulating plant physiological pathways includes: Oligosaccharide fragments released by lysozyme from the hydrolysis of pathogen cell walls act as signaling molecules, simultaneously activating plant disease resistance pathways, quality improvement pathways, and yield increase pathways by binding to the plant cell wall-associated kinase (WAK) receptor.

[0032] The aforementioned synchronous activation includes: Activate the MAPK cascade antiviral pathway; Activation of MYB transcription factor to induce the synthesis of secondary metabolites such as anthocyanins; Promote EXPANSIN gene expression to enhance cell wall relaxation and nutrient transport efficiency.

[0033] An application of a lysozyme-nanomobionic system in improving crop quality and efficiency, based on the aforementioned lysozyme-nanomobionic system, with different formulations and modification strategies adapted according to crop type, including: For gramineous crops, the suitable formulation is water-dispersible granules, and the surface of the biomimetic delivery body is modified with sodium lignosulfonate. For Solanaceae crops, the suitable formulation is nanoemulsion, with pectin lyase added as a synergist; For fruit trees, the appropriate formulation is a gel injected into the trunk, and the osmotic pressure of the system is adjusted to -0.8 MPa; For tuber crops, the suitable formulation is seed potato coated microspheres, which are loaded with gibberellin A4 in the system.

[0034] The crops mentioned include rice, tomatoes, citrus, or potatoes. The application of the lysozyme-nanomobionic system can increase crop yield, improve crop quality indicators, and reduce the loss rate caused by biotic or abiotic stress.

[0035] The statistical data after the implementation of this application is as follows: Table 1 - Implementation Data

[0036] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A lysozyme-nanobionic system based on cell wall signal regulation, characterized in that, The lysozyme-nanobionic system includes a core engine, a bionic delivery system, and an enhancement module. The core engine comprises a broad-spectrum lysozyme and a cell wall signal amplifier. The broad-spectrum lysozyme accounts for 0.8%-2.2% of the lysozyme-nanomobionic system by weight, and the cell wall signal amplifier accounts for 0.05%-0.15% of the lysozyme-nanomobionic system by weight. The biomimetic delivery system comprises plant exosome-mimicking vesicles and a pH / ROS dual-response gating system, wherein the plant exosome-mimicking vesicles account for 1.5%-4% by weight of the lysozyme-nanomomimetic system. The enhancement module includes a nano-silicon photosynthetic engine and a stress-resistance factor. The nano-silicon photosynthetic engine accounts for 0.001%-0.005% of the lysozyme-nano-bionic system by weight, and the stress-resistance factor accounts for 0.3%-0.9% of the lysozyme-nano-bionic system by weight. The remaining components of the lysozyme-nanobionic system are auxiliary materials.

2. The lysozyme-nanobionic system based on cell wall signal regulation according to claim 1, characterized in that, The broad-spectrum lysozyme is a chimeric lysozyme, which has dual active centers of plant chitinase and glucanase, and can hydrolyze the chitin and glucan components of the cell wall of pathogens. The dual active centers are obtained through gene chimerism technology. The cell wall signal amplifier is an oligogalacturonic acid fragment with a degree of polymerization (DP) of 6-8.

3. The lysozyme-nanobionic system based on cell wall signal regulation according to claim 1, characterized in that, The plant exosome-like vesicles are surface-modified with sodium lignin sulfonate or pectin lyase synergist, and the plant exosome-like vesicles are composed of soybean phospholipids and sterols in a weight ratio of 7:3, with a particle size of 80±10nm. The pH / ROS dual-response gating is composed of a polydopamine-hyaluronic acid composite membrane, and the pH / ROS dual-response gating response conditions are as follows: The polydopamine layer dissolves at pH values ​​below 5.5; Under conditions of reactive oxygen species (ROS) bursts, hyaluronic acid chains break.

4. The lysozyme-nanobionic system based on cell wall signal regulation according to claim 1, characterized in that, The nano-silicon photosynthetic engine is a chloroplast-targeted CdSe / ZnS quantum dot, and the stress-resistance factor is a proline-betaine eutectic.

5. A method for preparing a lysozyme-nanomobionic system, characterized in that, The preparation of the lysozyme-nanobionic system according to any one of claims 1-4 comprises the following steps: S1: Preparation of plant exosome-simulated vesicles: Soybean phospholipids and phytosterols were dissolved in an organic solvent in a certain proportion, and blank vesicles were prepared by thin-film evaporation-hydration method; S2: Loading active ingredients: Broad-spectrum lysozyme, cell wall signal amplifier, nano-silicon photosynthetic engine and stress resistance factor are dissolved or dispersed in buffer solution, and encapsulated in the blank vesicles obtained in step S1 by active drug loading or ultrasonic emulsification to obtain drug-loaded vesicles; S3: Constructing a response-gated membrane: Under weakly alkaline conditions, the drug-loaded vesicles obtained in step S2 are co-incubated with dopamine monomers to polymerize dopamine on the vesicle surface to form a polydopamine layer; subsequently, hyaluronic acid is modified onto the polydopamine layer by electrostatic adsorption or covalent crosslinking to form a polydopamine-hyaluronic acid composite membrane.

6. The method for preparing a lysozyme-nanomobionic system according to claim 5, characterized in that, In step S1, the organic solvent is a mixture of chloroform and methanol, and the film evaporation temperature is 35-45°C.

7. An application of a lysozyme-nanomobionic system in regulating plant physiological pathways, based on the lysozyme-nanomobionic system according to any one of claims 1-4, characterized in that, The applications of the lysozyme-nanomobionic system in regulating plant physiological pathways include: Oligosaccharide fragments released by lysozyme from the hydrolysis of pathogen cell walls act as signaling molecules, simultaneously activating plant disease resistance pathways, quality improvement pathways, and yield increase pathways by binding to the plant cell wall-associated kinase (WAK) receptor.

8. The application of the lysozyme-nanomobionic system according to claim 7 in regulating plant physiological pathways, characterized in that, The aforementioned synchronous activation includes: Activate the MAPK cascade antiviral pathway; Activation of MYB transcription factor to induce the synthesis of secondary metabolites such as anthocyanins; Promote EXPANSIN gene expression to enhance cell wall relaxation and nutrient transport efficiency.

9. The application of a lysozyme-nano-bionic system in improving crop quality and efficiency, based on the lysozyme-nano-bionic system according to any one of claims 1-4, characterized in that, Different formulations and modification strategies are adapted according to crop type, including: For gramineous crops, the suitable formulation is water-dispersible granules, and the surface of the biomimetic delivery body is modified with sodium lignosulfonate. For Solanaceae crops, the suitable formulation is nanoemulsion, with pectin lyase added as a synergist; For fruit trees, the appropriate formulation is a gel injected into the trunk, and the osmotic pressure of the system is adjusted to -0.8 MPa; For tuber crops, the suitable formulation is seed potato coated microspheres, which are loaded with gibberellin A4 in the system.

10. The application of the lysozyme-nanobionic system according to claim 9 in improving crop quality and efficiency, characterized in that, The crops mentioned include rice, tomatoes, citrus, or potatoes. The application of the lysozyme-nanomobionic system can increase crop yield, improve crop quality indicators, and reduce the loss rate caused by biotic or abiotic stress.

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