Preparation method and application of a pet disinfection composite biological enzyme quaternary ammonium salt preparation
By leveraging the synergistic effect of multiple components in a compound bio-enzyme quaternary ammonium salt formulation, the problems of narrow bactericidal spectrum, high irritation, and easy development of drug resistance in existing pet disinfectants are solved, achieving a highly efficient, safe, and gentle pet disinfection effect.
Patent Information
- Application Number
- CN202610474285.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-04-11
- Publication Date
- 2026-06-26
AI Technical Summary
Existing pet disinfectants suffer from problems such as narrow bactericidal spectrum, high irritation, easy development of drug resistance, and safety hazards. The market urgently needs a new type of pet disinfectant that is highly efficient, broad-spectrum, safe, and gentle.
It adopts a compound biological enzyme quaternary ammonium salt preparation, which is scientifically compounded with compound quaternary ammonium salt, lysozyme, lysostaphin, peony bud extract and sodium hyaluronate, etc., to form a multi-target synergistic bactericidal effect. Combined with low temperature dissolution and gentle mixing process, the biological activity of each component is preserved.
It achieves highly effective killing of a variety of pathogenic microorganisms, reduces irritation to pets' skin and mucous membranes, avoids health risks, and has high product stability and safety.
Smart Images
Figure SMS_2
Abstract
Description
Technical Field
[0001] This invention relates to the field of disinfectant technology, and in particular to a method for preparing and applying a compound biological enzyme quaternary ammonium salt preparation for pet disinfection. Background Technology
[0002] With the improvement of people's living standards, pet ownership has become a common lifestyle. While pets bring companionship and joy, their living environment can easily breed and spread various pathogenic microorganisms, such as bacteria (including drug-resistant bacteria), fungi, and viruses, posing a potential threat to the health of the pets themselves and their family members. Therefore, regular and effective disinfection of the pet's living environment, food and water utensils, and equipment is crucial.
[0003] Currently, common pet disinfectants on the market can be mainly divided into the following categories:
[0004] Chlorine-containing disinfectants (such as sodium hypochlorite): have a broad bactericidal spectrum, but have a strong irritating odor, are irritating to the respiratory tract and skin mucous membranes of pets, and are highly corrosive, damaging metal utensils, etc. After use, they need to be rinsed with plenty of water, otherwise the residue may be licked and ingested by pets, posing a safety hazard.
[0005] Alcohol-based disinfectants (such as ethanol): They kill bacteria quickly, but are volatile and ineffective against bacterial spores. They also have a degreasing effect on the skin, and long-term use may cause dry and cracked skin in pets.
[0006] Single quaternary ammonium salt disinfectants: They are mild and have low irritation, but their bactericidal spectrum is relatively narrow. In particular, they are not very effective against certain Gram-negative bacteria, fungi, and viruses. They are also easily affected by organic matter, and long-term use alone can induce bacteria to develop drug resistance.
[0007] With increasing awareness of pet health and public hygiene, the market urgently needs a new type of pet disinfectant that is highly effective, broad-spectrum, safe, mild, non-irritating, and unlikely to induce drug resistance. Bioenzymes (such as lysozyme and lysostaphylococcal lysozyme), as a novel antibacterial substance, are gaining attention due to their unique enzymatic mechanism, which specifically destroys bacterial cell walls, making them less prone to drug resistance and exhibiting good biocompatibility. Plant extracts (such as peony flower extract) are also a research hotspot due to their various natural antibacterial and anti-inflammatory active ingredients. However, no reports have yet documented a successful application of a scientifically formulated combination of quaternary ammonium salts, multiple bioenzymes, and plant extracts in the field of pet disinfection. Summary of the Invention
[0008] (a) Technical problems to be solved
[0009] To address the shortcomings of existing technologies, this invention achieves efficient, broad-spectrum, safe, and gentle disinfection effects through the synergistic effect of multiple components, making it particularly suitable for disinfecting pets and their living environments.
[0010] (II) Technical Solution
[0011] To achieve the above objectives, the present invention provides the following technical solution: a compound biological enzyme quaternary ammonium salt preparation for pet disinfection, comprising the following active ingredients and auxiliary raw materials:
[0012] Main active ingredient: 0.3%~0.5% compound quaternary ammonium salt;
[0013] Auxiliary ingredients: 0.01%~0.5% peony bud extract, 0.001%~0.1% lysozyme, 0.0001%~0.01% lysostaphylococcal enzyme, 0.01%~0.2% lactic acid, 0.001%~0.05% sodium hyaluronate, with the balance being water;
[0014] The composite quaternary ammonium salt is benzalkonium bromide and / or benzalkonium chloride, or a combination thereof.
[0015] As a preferred embodiment, the composite quaternary ammonium salt is benzalkonium bromide, with a content of 0.342%~0.418%.
[0016] As a preferred embodiment, the auxiliary raw material further includes 0.001% to 0.01% of plant enzymes, wherein the plant enzymes are selected from one or more of bromelain, papain, or figase.
[0017] As a preferred embodiment, the lysozyme is a microbial lysozyme with an enzyme activity of 1000~5000 U / mg; the lysostaphylococcal enzyme is a recombinant lysostaphylococcal enzyme with an enzyme activity of 500~2000 U / mg.
[0018] As a preferred embodiment, the peony bud extract is prepared by the following method: dried peony bud powder is taken, and extracted by reflux at 50-70°C for 1-3 hours using 70%-80% ethanol as solvent. After filtration, concentration and drying, the extract is obtained. The main active ingredients include phenolic acids, flavonoids and galloyl tannins.
[0019] A method for preparing a compound biological enzyme quaternary ammonium salt preparation includes the following steps:
[0020] S1. Under stirring conditions, the formulated amount of compound quaternary ammonium salt and lactic acid are dissolved in part of the water to obtain mixed solution A;
[0021] S2. Under constant temperature conditions of 20~40℃, dissolve the prescribed amounts of lysozyme, lysostaphin, sodium hyaluronate and optional plant enzymes in another portion of water to obtain mixed solution B.
[0022] S3. Dissolve the prescribed amount of peony bud extract in the remaining water to obtain mixed solution C;
[0023] S4. Under stirring conditions, slowly add the mixed solution B and mixed solution C to the mixed solution A, add the remaining water, and continue stirring until completely mixed and homogeneous;
[0024] S5. Allow to stand to defoam, filter and sterilize to obtain the compound biological enzyme quaternary ammonium salt preparation.
[0025] As a preferred embodiment, in step S2, the constant temperature condition is 25~35℃; in step S4, the stirring speed is 100~300rpm and the stirring time is 15~30 minutes.
[0026] The application of a compound biological enzyme quaternary ammonium salt preparation prepared by the method described above in the preparation of pet disinfection products, which are used to kill microorganisms on the surface of pet living environment and utensils.
[0027] As a preferred embodiment, the microorganisms include Salmonella Typhimurium, Escherichia coli, Staphylococcus aureus, Candida albicans, Trichophyton mentagrophytes, and poliovirus.
[0028] As a preferred embodiment, the disinfectant product is used for disinfecting pet food and drink utensils, pet food tools and equipment, and surfaces in pet business premises. When using the product, it should be diluted 200 to 500 times, and the contact time should be no less than 3 minutes.
[0029] (III) Beneficial Effects
[0030] Compared with the prior art, the present invention provides a method for preparing and applying a compound biological enzyme quaternary ammonium salt preparation for pet disinfection, which has the following beneficial effects:
[0031] I. This invention organically combines chemical disinfectants (compound quaternary ammonium salts), biological enzymes (lysozyme, lysostaphylococcal enzyme), plant extracts (peony bud extract), and organic acids (lactic acid) to form a multi-target synergistic bactericidal network. Through the superposition and complementarity of different mechanisms of action, a better synergistic effect is achieved, and the killing ability against a variety of pathogenic microorganisms is significantly better than any single component.
[0032] Second, this invention also significantly reduces the irritation of the formulation by introducing sodium hyaluronate and peony bud extract with anti-inflammatory activity, making it very gentle on pets' skin, eyes, and mucous membranes. The product ingredients are safe, avoiding the health risks that may be associated with traditional disinfectants, and the risk of pets licking it is low.
[0033] Third, the preparation method of this invention fully considers the heat and chemical sensitivity of biological enzymes and plant extracts, employing low-temperature dissolution and gentle mixing to maximize the preservation of the bioactivity of each component, ensuring the long-term stability and effectiveness of the product. Detailed Implementation
[0034] To better understand the purpose, structure, and function of this invention, the preparation method and application of a compound biological enzyme quaternary ammonium salt preparation for pet disinfection will be further explained below with reference to specific embodiments.
[0035] Example 1
[0036] This embodiment provides a compound biological enzyme quaternary ammonium salt preparation for pet disinfection, the formula of which is as follows (total 1L):
[0037] Main active ingredient: Benzalkonium bromide 4.0g (0.4% W / V);
[0038] Auxiliary ingredients: Peony bud extract 2.0g (0.2% W / V), microbial lysozyme (activity 2000U / mg) 0.5g (0.05% W / V), recombinant lysostaphylococcal lysozyme (activity 1000U / mg) 0.05g (0.005% W / V), lactic acid 1.0g (0.1% W / V), sodium hyaluronate 0.2g (0.02% W / V).
[0039] Solvent: Deionized water to bring the volume up to 1000ml.
[0040] Preparation method:
[0041] 1. Preparation of peony bud extract: Take dried peony buds, pulverize and sieve. Weigh 100g of powder, add 1000ml of 70% ethanol, reflux at 60℃ for 2h, and filter. Repeat the extraction once with the residue, and combine the filtrates. Concentrate the filtrate by vacuum rotary evaporation at 40℃ to a paste state, and freeze-dry to obtain peony bud extract powder for later use.
[0042] 2. Solution preparation:
[0043] Solution A: Add 4.0g benzalkonium bromide and 1.0g lactic acid to 500ml deionized water while stirring, and stir until completely dissolved.
[0044] Solution B: In 200ml of deionized water, at a temperature of 30℃, add 0.5g of lysozyme, 0.05g of lysostaphin and 0.2g of sodium hyaluronate in sequence, and stir gently until completely dissolved.
[0045] Solution C: Add 2.0g of peony bud extract to 200ml of deionized water and stir until completely dissolved.
[0046] 3. Mixing and Volume Adjustment: Under constant temperature of 30℃, stir solution A at 200 rpm, and slowly add solutions B and C sequentially. Make up the volume to 1000 ml with the remaining deionized water, and continue stirring for 20 minutes to ensure thorough mixing of all components.
[0047] 4. Post-treatment: Stop stirring and let stand for 30 minutes to defoam. Filter through a 0.22µm filter membrane for sterilization to obtain the compound biological enzyme quaternary ammonium salt preparation described in this invention. The product has a pH value of 4.5.
[0048] This embodiment scientifically combines a compound quaternary ammonium salt with various biological enzymes (lysozyme, lysostaphylococcal enzyme), plant extracts (peony bud extract), and organic acids (lactic acid) to fully leverage the synergistic effects of each component, overcoming the shortcomings of single-component formulations such as narrow bactericidal spectrum, easy induction of drug resistance, and high skin irritation. This formulation exhibits rapid and efficient killing effects against a variety of microorganisms, including Salmonella typhimurium, Escherichia coli, Staphylococcus aureus, Candida albicans, Trichophyton mentagrophytes, and poliovirus.
[0049] Example 2
[0050] This embodiment uses different formulations and their killing effects on major pathogens.
[0051] In this embodiment, different formulation groups were set up, with Staphylococcus aureus (ATCC 6538) and Escherichia coli (ATCC8099) as indicator bacteria. The synergistic bactericidal effect of the present invention was verified by referring to the quantitative bactericidal test method of suspension in the "Disinfection Technical Specification".
[0052] Preparation of test bacterial suspension: After activating the test bacterial strain, it was prepared with tryptone physiological saline (TPS) to a concentration of approximately 1× 10⁻⁶ bacteria. CFU / mL bacterial suspension.
[0053] Neutralizing agent identification: Preliminary tests have determined that a PBS solution containing 0.5% lecithin, 0.5% sodium thiosulfate, and 3% Tween-80 can be used as an effective neutralizing agent.
[0054] Bactericidal test: Under 20℃ water bath conditions, mix 0.5 ml of bacterial suspension with 0.5 ml of 3% bovine serum albumin (organic interfering agent). Add 4.0 ml of diluted disinfectant (each formulation stock solution diluted 250 times with sterile hard water). After 5 min of contact, immediately add 0.5 ml of the mixture to the 4.5 ml solution for neutralization. After 10 min of neutralization, count the viable bacteria and calculate the kill log value. The test was repeated 3 times. The positive control group used sterile hard water instead of disinfectant.
[0055] Experimental Groups:
[0056] Control group 1: 0.4% benzalkonium bromide (a single quaternary ammonium salt).
[0057] Control group 2: 0.05% lysozyme + 0.005% lysostaphin (complex enzyme).
[0058] Control group 3: 0.2% peony bud extract.
[0059] Experimental group: The formulation of Example 1 of this invention (containing 0.4% benzalkonium bromide, 0.05% lysozyme, 0.005% lysostaphin, 0.2% peony bud extract, 0.1% lactic acid, and 0.02% sodium hyaluronate).
[0060] Experimental results: The experimental results are shown in Table 1 below.
[0061] Table 1
[0062]
[0063] Results analysis:
[0064] Table 1 shows that the single component had limited killing effect on the two indicator bacteria, especially on Gram-negative bacteria (Escherichia coli). However, the experimental group of this invention, through multi-component compounding, achieved a logarithmic kill value greater than 5.00 against both Staphylococcus aureus (Gram-positive bacteria) and Escherichia coli (Gram-negative bacteria), demonstrating excellent killing effect. This indicates that there is a significant synergistic effect among the components, effectively overcoming the bactericidal defects of single components through multi-target attack, and achieving broad-spectrum and highly efficient bactericidal activity.
[0065] Example 3
[0066] The compound biological enzyme quaternary ammonium salt preparation prepared in Example 1 was diluted 250 times according to the instructions, and the following pet products were subjected to disinfection tests:
[0067] Pet food bowl disinfection: Five used pet food bowls were taken, washed, and soaked in a diluted solution for 10 minutes. Then, sterile cotton swabs were used to sample the inner wall of the bowls for viable bacteria counting. Results showed that the average total bacterial count decreased by more than 99.99% compared to before disinfection.
[0068] Pet toy disinfection: Take 5 plush pet toys, spray them evenly with diluted solution until the surface is moist, and leave for 10 minutes. Then, use a sterile cotton swab to sample the surface of the toys and count the viable bacteria. The results showed that the average total bacterial count decreased by more than 99.9% compared with before disinfection.
[0069] Dog cage surface disinfection: Take the metal bars of 5 dog cages and wipe them with a towel soaked in diluted solution, leaving the area for 5 minutes. Then, use sterile cotton swabs to sample the wiped bar surface for viable bacteria counting. Results showed that the average total bacterial count decreased by more than 99.9% compared to before disinfection.
[0070] The results of on-site disinfection tests show that the preparation of the present invention, after dilution, has a very good killing effect on the natural bacteria on the surface of common items in the pet's living environment, and is easy to use with a mild odor.
[0071] It is understood that the present invention has been described through some embodiments, and those skilled in the art will recognize that various changes or equivalent substitutions can be made to these features and embodiments without departing from the spirit and scope of the invention. Furthermore, under the teachings of the present invention, these features and embodiments can be modified to adapt to specific situations and materials without departing from the spirit and scope of the invention. Therefore, the present invention is not limited to the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of this application are within the protection scope of the present invention.
Claims
1. A pet disinfectant complex bio-enzyme quaternary ammonium salt formulation, characterized in that, It consists of the following active ingredients and auxiliary materials by weight and volume percentage: Main active ingredient: 0.3%~0.5% compound quaternary ammonium salt; Auxiliary ingredients: 0.01%~0.5% peony bud extract, 0.001%~0.1% lysozyme, 0.0001%~0.01% lysostaphylococcal enzyme, 0.01%~0.2% lactic acid, 0.001%~0.05% sodium hyaluronate, with the balance being water; The composite quaternary ammonium salt is benzalkonium bromide and / or benzalkonium chloride, or a combination thereof.
2. The pet disinfectant composite bio-enzyme quaternary ammonium salt preparation according to claim 1, characterized in that, The composite quaternary ammonium salt is benzalkonium bromide, with a content of 0.342%~0.418%.
3. The pet disinfectant composite bio-enzyme quaternary ammonium salt preparation according to claim 1, characterized in that, The auxiliary raw materials also include 0.001% to 0.01% of plant enzymes, which are selected from one or more of bromelain, papain, or figase.
4. The compound biological enzyme quaternary ammonium salt preparation for pet disinfection according to claim 1, characterized in that, The lysozyme is a microbial lysozyme with an enzyme activity of 1000~5000 U / mg; the lysostaphylococcal enzyme is a recombinant lysostaphylococcal enzyme with an enzyme activity of 500~2000 U / mg.
5. The preparation method and application of a compound biological enzyme quaternary ammonium salt preparation for pet disinfection according to claim 1, characterized in that, The peony bud extract is prepared by the following method: dried peony bud powder is taken, and extracted by reflux at 50-70°C for 1-3 hours using 70%-80% ethanol as solvent. After filtration, concentration and drying, the extract is obtained. The main active ingredients include phenolic acids, flavonoids and galloyl tannins.
6. A method for preparing a compound biological enzyme quaternary ammonium salt preparation as described in any one of claims 1-5, characterized in that, Includes the following steps: S1. Under stirring conditions, the formulated amount of compound quaternary ammonium salt and lactic acid are dissolved in part of the water to obtain mixed solution A; S2. Under constant temperature conditions of 20~40℃, dissolve the prescribed amounts of lysozyme, lysostaphin, sodium hyaluronate and optional plant enzymes in another portion of water to obtain mixed solution B. S3. Dissolve the prescribed amount of peony bud extract in the remaining water to obtain mixed solution C; S4. Under stirring conditions, slowly add the mixed solution B and mixed solution C to the mixed solution A, add the remaining water, and continue stirring until completely mixed and homogeneous; S5. Allow to stand to defoam, filter and sterilize to obtain the compound biological enzyme quaternary ammonium salt preparation.
7. The method for preparing the compound biological enzyme quaternary ammonium salt preparation according to claim 6, characterized in that... In step S2, the constant temperature condition is 25~35℃; in step S4, the stirring speed is 100~300rpm and the stirring time is 15~30 minutes.
8. The application of a compound bio-enzyme quaternary ammonium salt preparation obtained by the preparation method as described in claim 6 in the preparation of pet disinfection products, characterized in that, Disinfectant products are used to kill microorganisms on the surfaces of pets' living environment and utensils.
9. The application of the compound biological enzyme quaternary ammonium salt preparation according to claim 8 in the preparation of pet disinfection products, characterized in that, The microorganisms include Salmonella Typhimurium, Escherichia coli, Staphylococcus aureus, Candida albicans, Trichophyton mentagrophytes, and poliovirus.
10. The application of the compound biological enzyme quaternary ammonium salt preparation according to claim 8 in the preparation of pet disinfection products, characterized in that, The disinfectant product is used for disinfecting pet food and drink utensils, pet food tools and equipment, and surfaces in pet business premises. When using the product, it should be diluted 200 to 500 times, and the contact time should be no less than 3 minutes.