A genetic fragment for regulating leaf angle size on corn ear, a molecular marker and application thereof

By designing molecular markers and primer pairs in specific regions of the maize B73 reference genome, the problem of genetic regulation of leaf angle was solved, enabling efficient breeding of maize varieties with compact leaf angles on the ear and improving breeding efficiency.

CN122279072APending Publication Date: 2026-06-26HENAN AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HENAN AGRICULTURAL UNIVERSITY
Filing Date
2025-11-29
Publication Date
2026-06-26

AI Technical Summary

Technical Problem

Leaf angle is a typical quantitative trait, which is jointly regulated by multiple genes and has a complex genetic basis. Its phenotype is easily affected by environmental conditions. Current technology makes it difficult to accurately identify the relevant regulatory genes, which makes it difficult to breed superior maize varieties with compact leaf angles on the ear.

Method used

A genetic fragment and molecular marker that regulates the leaf angle on a maize ear are provided. The fragment is located in the 201960860-201960877 bp region of chromosome 2 on RefGen_v4 of the maize B73 reference genome. Primer pairs were designed for PCR amplification, and the amplification products were detected by agarose gel electrophoresis to identify the leaf angle.

Benefits of technology

This study has enabled the development of an efficient molecular-assisted selection method, significantly improving breeding selection efficiency and enabling the breeding of maize varieties with smaller angles between the upper leaves and the ears, thus promoting the breeding process of high-yielding and dense-planting tolerant maize varieties.

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Abstract

This invention relates to the field of biotechnology, specifically disclosing a genetic fragment, molecular marker, and application for regulating the angle between ears of maize leaves. The genetic fragment is located on chromosome 2 of the maize B73 reference genome (RefGen_v4), between 201960860 and 201960877 bp, with a physical location interval of less than 20 bp, constituting a key genetic locus (named LA1) regulating the angle between ears of leaves. This locus contains the genetic variation sites rs_2_201960860 and rs_2_201960877. This invention significantly improves breeding selection efficiency, provides a powerful tool for breeding high-yielding and density-tolerant maize varieties, and has significant application value and promising prospects for achieving high and stable maize yields.
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