A repairing and soothing composition, its preparation method and application

Abstract

This invention belongs to the technical field of repair and soothing compositions, and discloses a method for preparing and applying such a composition. The method for preparing the soothing and repairing composition includes the following steps: S1. Enzymatic hydrolysis: Add a compound enzyme to gentian root and forsythia respectively for enzymatic extraction to obtain gentian root suspension and forsythia suspension; S2. Three-phase extraction: Add PEG-400 and an ionic liquid to the gentian root suspension and forsythia suspension respectively, stir evenly to form a water-PEG-400-ionic liquid three-phase extraction system for extraction, and concentrate to obtain concentrated gentian root extract and concentrated forsythia extract; S3. Low-temperature directional fusion: Add the concentrated gentian root extract and concentrated forsythia extract in proportion, then add butylene glycol, stir evenly, and allow to fuse at 1-10℃. The soothing and repairing composition of this invention has a high retention rate of active ingredients, high stability, and excellent skin soothing effect.

Description

Technical Field

[0001] This invention relates to the technical field of repair and soothing compositions, and more specifically, to a repair and soothing composition, its preparation method, and its application. Background Technology

[0002] The soothing and repairing composition uses gentian root and forsythia as raw materials. The preparation process includes raw material pretreatment, single-solvent ethanol reflux extraction or pure water extraction, filtration and concentration, and simple physical mixing at room temperature to obtain the soothing and repairing composition. Its active ingredients, such as gentiopicrin and forsythoside, can soothe and repair the skin to a certain extent. However, existing methods for preparing the soothing and repairing composition have the following drawbacks: (1) Low content of active ingredients.

[0003] Pure water extraction or ethanol reflux extraction both require high-temperature extraction at 85-95℃. Gentianoside and forsythoside are heat-sensitive active substances. The cyclic molecular structure of these two active ingredients is directly destroyed by high temperature, resulting in a high loss rate. Therefore, their content in the final soothing and repairing composition is low. Ethanol reflux extraction with a single solvent can easily lead to denaturation of gentiopicrin and forsythoside, while pure water extraction with a single solvent can only extract water-soluble gentiopicrin. Because the amount of lipid-soluble forsythoside dissolved is extremely low, a large amount of forsythoside is left in the extraction residue, so the content in the final soothing and repairing composition is low.

[0004] (2) Poor stability.

[0005] After filtration and concentration, the extracts of gentian root and forsythia are simply mixed by physical stirring. The active molecules of gentiopicrin and forsythoside are randomly distributed in the system and are easily affected by external temperature and pH fluctuations.

[0006] (3) In the obtained soothing and repairing composition, the molecules of gentiopicrin and forsythoside are randomly distributed in the system, and the soothing effect on the skin is not ideal.

[0007] Gentianoside tends to form water-soluble macromolecular clusters, while forsythoside tends to precipitate microcrystals, making it difficult for active molecules to penetrate the nanoscale intercellular spaces of the stratum corneum. As a result, they remain on the surface and have difficulty penetrating into the deep layers of the skin. Gentianoside is strongly hydrophilic while free forsythoside is more lipophilic. Due to their large difference in polarity, they diffuse asynchronously on the skin and cannot act on the skin inflammation targets and barrier targets at the same time, making it difficult to achieve synchronous soothing and repair. Summary of the Invention

[0008] This invention provides a repairing and soothing composition with minimal loss of active ingredients, high stability, and superior skin-soothing effects compared to existing technologies.

[0009] Another object of the present invention is to provide a method for preparing the repairing and soothing composition.

[0010] Another object of the present invention is to provide the application of the repairing and soothing composition in the field of skin care.

[0011] To solve the above-mentioned technical problems, the technical solution provided by the present invention is as follows: A soothing and repairing composition containing gentian root extract, forsythia extract and butylene glycol.

[0012] The soothing and repairing composition of this invention has a high retention rate of active ingredients, high stability, and excellent soothing effect on the skin.

[0013] Preferably, the mass fractions of each component in the soothing and repairing composition are: Gentian root extract 8-15 parts, Forsythia extract 12-24 parts, Butylene glycol 5-10 parts.

[0014] The present invention also provides a method for preparing the soothing and repairing composition according to any one of the above claims, comprising the following steps: S1. Compound enzyme hydrolysis: Add compound enzyme to gentian root and forsythia respectively, and carry out enzymatic hydrolysis extraction at 37~39℃ to obtain gentian root suspension and forsythia suspension. S2. Three-phase system extraction: PEG-400 and ionic liquid were added to the gentian root suspension and the forsythia suspension respectively, and stirred evenly to form a water-PEG-400-ionic liquid three-phase extraction system. Extraction was carried out at 25~32℃, and concentrated gentian root extract and forsythia extract were obtained after concentration; the ionic liquid was 1-butyl-3-methylimidazolium tetrafluoroborate. S3. Low-temperature directional fusion: Add concentrated extracts of gentian root and forsythia root in proportion, then add butylene glycol, stir evenly, let stand at 1-10℃ to fuse, and filter to obtain a soothing and repairing composition.

[0015] The preparation method of the soothing and repairing combination described in this invention has the following technical effects: (1) High content of active ingredients.

[0016] S1 is extracted by enzymatic hydrolysis at 37~39℃, and S2 is extracted at 25~32℃. The present invention uses low temperature extraction, which does not destroy the cyclic molecular structure of the two active ingredients, gentiopicrin and forsythoside, and the loss rate is low. Therefore, the content in the final soothing and repairing composition is high. S2. A three-phase extraction system of water-PEG-400-ionic liquid is adopted, in which the aqueous phase can extract water-soluble gentiopicrin, the PEG-400 organic phase can fully dissolve fat-soluble forsythosides without causing denaturation of gentiopicrin and forsythosides, and the ionic liquid further promotes the extraction of gentiopicrin and forsythosides, so the content of gentiopicrin and forsythosides is high in the final soothing and repairing composition.

[0017] (2) High stability.

[0018] After simple physical stirring, the S3 gentian root and forsythia extracts are further subjected to a static fusion step at a low temperature of 1~10℃. This step promotes the orderly combination of gentiopicrin and forsythoside molecules through hydrogen bonds and hydrophobic interactions, forming a structurally stable composite molecule. The composite molecule is well-arranged, has strong anti-interference ability, and is not easily affected by external temperature and pH fluctuations.

[0019] (3) Since S3. gentiopicroside and forsythoside molecules combine to form a structurally stable complex molecule, the resulting soothing and repairing composition has an excellent skin-soothing effect.

[0020] It can inhibit the formation of water-soluble macromolecular clusters of gentiopicroside and prevent the precipitation of microcrystals of forsythoside. The composite molecules have small particle size and can easily penetrate the nanoscale intercellular spaces of the stratum corneum. Therefore, gentiopicroside and forsythoside can penetrate into the deep layers of the skin. The highly hydrophilic gentiopicroside and the partially lipophilic forsythoside are bound together in an orderly manner by hydrogen bonds and hydrophobic interactions, overcoming the shortcomings of the large polarity difference between the two in existing technologies. Therefore, when applied to the skin, they diffuse and move synchronously and evenly, and can act on the skin inflammation target and barrier target at the same time, forming synchronous soothing and repair, with excellent soothing and barrier repair effects.

[0021] Preferably, the compound enzyme added to the S1 *Lysimachia christinae* root contains cellulase and xylanase, and the compound enzyme added to the *Forsythia suspensa* contains pectinase and hemicellulase.

[0022] Gentian root has a high fiber content, and its cell wall components mainly contain cellulose and xylan. Adding cellulase and xylanase can specifically decompose these components, break down the cell wall barrier, and promote the dissolution of water-soluble gentiopicrin. Forsythia has a high pectin content, and its cell wall components mainly contain pectin and hemicellulose. Adding pectinase and hemicellulase can specifically break down these components and release fat-soluble forsythosides.

[0023] In S1 of this invention, after adding a compound enzyme to the gentian root suspension and the forsythia suspension, calcium chloride is then added to the gentian root mixture and the forsythia mixture as an enzyme activator; the amount of calcium chloride added is 0.04% to 0.06% of the mass of each mixture.

[0024] Calcium chloride, as an enzyme activator, can enhance the activity of complex enzymes, accelerate enzymatic hydrolysis, and further improve the dissolution rate of active ingredients.

[0025] More preferably, the compound enzyme added to the S1 *Lysimachia christinae* root contains cellulase and xylanase in a mass ratio of (2.5~3.5):1; the compound enzyme added to the *Forsythia suspensa* contains pectinase and hemicellulase in a mass ratio of (1.5~2.5):1.

[0026] Preferably, the S1 enzymatic hydrolysis extraction time is 2-3 hours.

[0027] Preferably, the S2 gentian root suspension or forsythia suspension contains PEG-400 at a mass of 28%-32% of the suspension mass and ionic liquid at a mass of 4%-6% of the suspension mass.

[0028] Preferably, the S2 ultrasonic-microwave process is as follows: ultrasonic power is 180-220W, ultrasonic temperature is 25-30℃, then microwave power is set to 90-110W, microwave temperature is set to 28-32℃, and the total extraction time is 15-25min.

[0029] In the S2 extraction process described in this invention, an ultrasonic-microwave process is applied. The specific parameters of the S2 ultrasonic-microwave process are as follows: ultrasonic power is 180-220W, ultrasonic temperature is 25-30℃, and ultrasonic time is 10-20min. Then, the microwave power is set to 90-110W, microwave temperature is 28-32℃, and microwave time is 3-8min. This process is repeated once, with a total extraction time of 15-25min. During the ultrasonic-microwave process, the mixture is stirred once every 3-6min.

[0030] The present invention also provides the application of the soothing and repairing composition described in any of the above claims in the field of skin care. Detailed Implementation

[0031] The present invention will be further described in detail below with reference to specific embodiments.

[0032] Example 1 A soothing and repairing composition comprising gentian root extract, forsythia extract and butylene glycol, wherein the mass parts of each component are: 12 parts gentian root extract, 18 parts forsythia extract and 8 parts butylene glycol.

[0033] To prepare a soothing and repairing composition, the following steps are included: S1. Enzymatic hydrolysis with a compound enzyme containing cellulase and xylanase (mass ratio of cellulase to xylanase 3:1, cellulase activity ≥5000U / g, xylanase activity ≥4000U / g) was added to the gentian root to obtain a gentian root mixture. Then, 0.05% (by weight of the suspension) of calcium chloride, an enzyme activator, was added to the gentian root mixture. After stirring evenly, the mixture was placed in a constant temperature water bath shaker at 38℃ and 110~130r / min for 2~3h. Finally, after the enzymatic hydrolysis was completed, the gentian root mixture was heated in a 90℃ water bath for 15min to inactivate the compound enzyme. After cooling to room temperature, a gentian root suspension was obtained. Add a compound enzyme containing pectinase and hemicellulase (mass ratio of pectinase to hemicellulase 2:1, pectinase activity ≥3000U / g, hemicellulase activity ≥3500U / g) to Forsythia suspensa to obtain a Forsythia suspensa mixture. Then add 0.05% (by weight of the suspension) of calcium chloride, an enzyme activator, to the Forsythia suspensa mixture. After stirring evenly, place it in a constant temperature water bath shaker. The enzymatic hydrolysis temperature is 38℃, the enzymatic hydrolysis speed is 110~130r / min, and the enzymatic hydrolysis time is 2~3h. Finally, after the enzymatic hydrolysis is completed, place the Forsythia suspensa mixture in a 90℃ water bath for 15min to inactivate the compound enzyme. Cool to room temperature to obtain a Forsythia suspensa suspension. S2. Three-phase system extraction: PEG-400 and ionic liquid were added to the gentian root suspension and the forsythia suspension respectively, and stirred evenly to form a water-PEG-400-ionic liquid three-phase extraction system. After concentration, gentian root concentrated extract and forsythia concentrated extract were obtained; the ionic liquid was 1-butyl-3-methylimidazolium tetrafluoroborate. The S2 gentian root suspension or forsythia suspension contains PEG-400 at 30% of its respective suspension mass and ionic liquid at 5% of its respective suspension mass. During the S2 extraction process, an ultrasonic-microwave process is applied. The specific parameters of the S2 ultrasonic-microwave process are: ultrasonic power of 200W, ultrasonic temperature of 27℃, ultrasonic time of 15min, and then microwave power of 100W, microwave temperature of 30℃, and microwave time of 5min are set. This process is repeated once, and the total extraction time is 20min. During the ultrasonic-microwave process, the mixture is stirred once every 4min. Then, after ultrasonic-microwave extraction, the extract was transferred to a high-speed centrifuge and centrifuged at 8000 r / min for 20 min. The supernatant of the gentian root extract and the supernatant of the forsythia extract were collected separately, and the precipitate and impurities were discarded. Finally, the supernatant of the gentian root extract and the supernatant of the forsythia extract were placed in a low-temperature vacuum concentrator and concentrated to 1 / 6 to 1 / 4 of their original volume to obtain concentrated gentian root extract and concentrated forsythia extract. S3. Low-temperature directional fusion: In a sterile operating table, add concentrated extracts of gentian root and forsythia root in proportion to a sterile mixing tank, then add butylene glycol and stir at 70-90 r / min for 13-17 min; then place the mixing tank in a low-temperature environment of 4℃ and let it stand for fusion for 4 hours; after fusion, filter through a 0.22μm microporous membrane to remove trace impurities and bacteria to obtain a soothing and repairing skin composition.

[0034] Example 2 The composition is basically the same as in Example 1, except that the mass parts of each component in the soothing and repairing composition are: 8 parts gentian root extract, 12 parts forsythia extract, and 5 parts butylene glycol.

[0035] Example 3 The composition is basically the same as in Example 1, except that the mass parts of each component in the soothing and repairing composition are: 15 parts of gentian root extract, 24 parts of forsythia extract, and 10 parts of butylene glycol.

[0036] Example 4 The method is basically the same as in Example 1, except that in the preparation method of the soothing and repairing composition, S1. a complex enzyme containing cellulase and xylanase is added to the gentian root (the mass ratio of cellulase to xylanase is 2.5:1); and a complex enzyme containing pectinase and hemicellulase is added to the forsythia (the mass ratio of pectinase to hemicellulase is 1.5:1).

[0037] Example 5 The method is basically the same as in Example 1, except that in the preparation method of the soothing and repairing composition, S1. a complex enzyme containing cellulase and xylanase is added to the gentian root (the mass ratio of cellulase to xylanase is 3.5:1); and a complex enzyme containing pectinase and hemicellulase is added to the forsythia (the mass ratio of pectinase to hemicellulase is 2.5:1).

[0038] Example 6 The method is basically the same as in Example 1, except that in the preparation method of the soothing and repairing composition, S1. a complex enzyme containing cellulase is added to the gentian root, that is, the complex enzyme does not contain xylanase; a complex enzyme containing pectinase is added to the forsythia, that is, the complex enzyme does not contain hemicellulase.

[0039] Example 7 The method is basically the same as in Example 1, except that in the preparation method of the soothing and repairing composition, S1. a complex enzyme containing xylanase is added to the gentian root, that is, the complex enzyme does not contain cellulase; a complex enzyme containing hemicellulase is added to the forsythia, that is, the complex enzyme does not contain pectinase.

[0040] Example 8 The method is basically the same as in Example 1, except that in the preparation method of the soothing and repairing composition, after adding the compound enzyme to S1. Gentian root and Forsythia, calcium chloride, the enzyme activator, is not added.

[0041] Example 9 Similar to Example 1, except that in the preparation method of the soothing and repairing composition, in S2, PEG-400 is added to the gentian root suspension or forsythia suspension at a mass of 28% of the respective suspension mass, and ionic liquid is added at a mass of 4% of the respective suspension mass; during the extraction process in S2, an ultrasonic-microwave process is applied with the following parameters: ultrasonic power of 180W, ultrasonic temperature of 25℃, ultrasonic time of 10min, and then microwave power of 90W, microwave temperature of 28℃, and microwave time of 3min are set, alternating once, for a total extraction time of 15min, and stirring is performed once every 3min during the ultrasonic-microwave process.

[0042] Example 10 Similar to Example 1, except that in the preparation method of the soothing and repairing composition, in S2, PEG-400 is added to the gentian root suspension or forsythia suspension at a mass of 32% of the respective suspension mass, and ionic liquid is added at a mass of 6% of the respective suspension mass; during the extraction process in S2, an ultrasonic-microwave process is applied with the following parameters: ultrasonic power of 220W, ultrasonic temperature of 30℃, ultrasonic time of 20min, and then microwave power of 110W, microwave temperature of 32℃, and microwave time of 8min are set, alternating once, for a total extraction time of 25min, and stirring is performed once every 6min during the ultrasonic-microwave process.

[0043] Example 11 The method is basically the same as in Example 1, except that in the preparation method of the soothing and repairing composition, S3. the low temperature fusion temperature is 1°C and the standing fusion time is 3.5h.

[0044] Example 12 The method is basically the same as in Example 1, except that in the preparation method of the soothing and repairing composition, S3. the low-temperature fusion temperature is 10°C and the standing fusion time is 4.5h.

[0045] Comparative Example 1 The method is basically the same as in Example 1, except that the preparation method of the soothing and repairing composition does not include the S1 enzymatic hydrolysis step. Instead, the gentian root and forsythia are directly extracted in the S2 three-phase system after being crushed and soaked.

[0046] Comparative Example 2 The method is basically the same as in Example 1, except that in the preparation method of the soothing and repairing composition, S2. three-phase system extraction is performed, and the extraction medium is only water-ionic liquid, without the addition of PEG-400.

[0047] Comparative Example 3 The method is basically the same as in Example 1, except that in the preparation method of the soothing and repairing composition, S2. three-phase system extraction is performed, and the extraction medium is only water-PEG-400, without the addition of ionic liquid.

[0048] Comparative Example 4 The method is basically the same as in Example 1, except that in the preparation method of the soothing and repairing composition, S2. three-phase system extraction is performed, and the extraction medium is only water, without PEG-400 or ionic liquid.

[0049] Comparative Example 5 The method is basically the same as in Example 1, except that in the preparation method of the soothing and repairing composition, S3. Low-temperature fusion temperature is 0°C.

[0050] Comparative Example 6 The method is basically the same as in Example 1, except that in the preparation method of the soothing and repairing composition, S3. The low-temperature fusion temperature is 13°C.

[0051] Performance testing: 1. Content of active ingredients in the soothing and repairing composition The active ingredients in the soothing and repairing composition were identified as gentiopicrin and forsythoside. The specific steps for detecting the content of gentiopicrin and forsythoside are as follows: (1) Instruments and reagents Instruments: High-performance liquid chromatograph, ultraviolet detector, analytical balance, high-speed centrifuge, ultrasonic cleaner, 0.22μm organic filter membrane, volumetric flasks Reagents: Gentianoside standard, Forsythoside standard, Acetonitrile (chromatographic grade), Methanol (chromatographic grade), Ultrapure water (2) Chromatographic conditions Chromatographic column: C18 reversed-phase column (250mm × 4.6mm, 5μm) Mobile phase: Acetonitrile - Water = 25:75 (volume ratio) Detection wavelengths: Gentianin 270nm, Forsythoside 230nm Flow rate: 1.0 mL / min Column temperature: 30℃ Injection volume: 20 μL Washing method: isocratic washing (3) Preparation of standard solutions Accurately weigh 10 mg each of gentiopicrin standard and forsythoside standard dried to constant weight, and place them in 100 mL volumetric flasks respectively. Dissolve in methanol and dilute to the mark to prepare a 0.1 mg / mL single standard stock solution; A series of standard solutions were prepared by gradient dilution, and standard curves for gentiopicrin and forsythoside were plotted. The regression equations were used to calculate the content.

[0052] (4) Preparation of test solution Accurately weigh 2.0 g of each example / comparative example soothing and repairing composition sample and place it in a 50 mL stoppered conical flask; Add 25 mL of methanol, weigh, extract by sonication for 30 min, cool and then make up the weight loss with methanol; Centrifuge at 8000 r / min for 15 min and collect the supernatant; The supernatant was filtered through a 0.22 μm organic filter membrane, and the filtrate was the test solution.

[0053] (5) Detection and calculation methods Inject the standard solution and each test sample solution sequentially, record the peak area, substitute it into the standard curve regression equation, calculate the mass percentage content of gentiopicrin and forsythoside in the sample, and perform parallel testing on each group of samples three times and take the average value.

[0054] The results of the detection of gentiopicrin and forsythoside content in the soothing and repairing compositions obtained in Examples 1-12 and Comparative Examples 1-6 are shown in Table 1.

[0055] Table 1. Content of active ingredients in the soothing and repairing compositions obtained in Examples 1-12 and Comparative Examples 1-6

[0056] 2. Stability of the soothing and repairing composition The evaluation adopts a three-dimensional approach, which includes heat resistance, cold resistance, and room temperature retention stability. The core testing indicators are appearance, stratification and precipitation, transmittance change rate, and active ingredient retention rate. The key assessments are whether there is stratification, turbidity, crystallization, discoloration, precipitation, and the retention ratio of gentiopicrin and forsythoside after storage.

[0057] The specific testing methods and steps for the stability of the soothing and repairing compositions obtained in each embodiment and comparative example are as follows: (1) Sample preparation The soothing and repairing compositions prepared in Examples 1-12 and Comparative Examples 1-6 were dispensed into transparent sealed sample bottles of the same size, 50g per bottle, with 3 replicates per group, for later use.

[0058] (2) Stability test of samples at room temperature Conditions: Temperature 25℃±2℃, relative humidity 60%±5%, sealed and left to stand for 90 days. Observation points: 0d, 30d, 60d, 90d, record appearance, presence of stratification / turbidity / crystallization / precipitation; at the same time, sample and test the content of gentiopicrin and forsythoside, and calculate the retention rate of active ingredients after 90 days.

[0059] (3) Heat resistance stability test Conditions: 45℃±1℃ constant temperature and humidity chamber, sealed and left to stand for 30 days. After the experiment, remove and allow to return to room temperature, observe appearance (layering, turbidity, precipitation), and measure transmittance and retention rate of active ingredients.

[0060] (4) Cold resistance stability test Conditions: Low temperature freezer -10℃±1℃, sealed and left to stand for 30 days. Take it out and let it warm to room temperature naturally for 2 hours, then observe whether it becomes cloudy, crystals precipitate, separates into layers, or flocculates.

[0061] (5) Operation steps for testing items Visual inspection for appearance: color uniformity, whether there is layering, presence of white crystal precipitation, flocculent matter, bottom sediment, turbidity, and discoloration. Grading: Excellent (no change, uniform and transparent), Good (slightly turbid, no layering or sediment), Poor (obvious layering / precipitation / large amount of sediment).

[0062] Transmittance was measured using an ultraviolet spectrophotometer with pure water as a blank control. The transmittance of the sample was measured at a wavelength of 550 nm, and the decrease in transmittance before and after storage was calculated. The smaller the decrease, the better the stability.

[0063] The retention rate of active ingredients was calculated using the HPLC method for detecting the content of active ingredients, and the contents of gentiopicrin and forsythoside were determined at the initial 0 days and after the experiment: Retention rate of active ingredients (%) = Content after storage / Initial content × 100%.

[0064] The stability test results of the soothing and repairing compositions obtained in Examples 1-12 and Comparative Examples 1-6 are shown in Table 2.

[0065] Table 2. Stability of the soothing and repairing compositions obtained in Examples 1-12 and Comparative Examples 1-6

[0066] 3. Soothing and Repairing Composition: Soothing and Repairing Effects Select classic evaluation indicators for soothing and repairing cosmetics: Soothing effects: In vitro RAW264.7 cell inflammation inhibition rate, human skin redness inhibition rate Repair effect: The rate of increase in skin stratum corneum moisture content and the rate of decrease in transepidermal water loss (TEWL) were based on Example 1. All groups were tested under the same conditions, and the data were matched to the differences in formulation and process variables.

[0067] The specific testing steps for the soothing and repairing effects of the soothing and repairing compositions obtained in each embodiment and comparative example are as follows: (a) In vitro cellular anti-inflammatory and soothing assay (RAW264.7 macrophages) (1) Experimental materials and instruments RAW264.7 cells, LPS lipopolysaccharide, CCK-8 kit, microplate reader, cell culture incubator, clean bench, and test solutions for each example / comparative example composition.

[0068] (2) Experimental procedures Cell seeding: RAW264.7 macrophages were seeded in 96-well plates and cultured to the logarithmic growth phase; Grouping: blank control group, model group (LPS-induced inflammation), and sample groups of each embodiment / comparative example; Sample preparation: The soothing and repairing compositions obtained in each example and comparative example were diluted with culture medium to a uniform mass concentration test solution and added to the corresponding well plate; Inflammation induction: Except for the control group, LPS was added to the other groups to establish an inflammation model; Constant temperature incubation: 37℃, 5% CO2 incubation for 24h; Incubate with CCK-8 reagent and measure absorbance at 450 nm using an ELISA reader; Calculate the inflammation suppression rate: Inflammation suppression rate = ×100% (II) Human Skin Soothing and Repairing Effects: Human Trial Testing (1) Subjects Ninety volunteers with sensitive skin were recruited and randomly divided into 18 groups (Examples 1–12, Comparative Examples 1–6), with five people in each group, and a control experiment was conducted on the left and right cheeks.

[0069] (2) Test conditions Constant temperature and humidity environment: temperature 22±2℃, humidity 55±5%, continuous trial for 28 days, apply once in the morning and once in the evening.

[0070] (3) Testing items and operating procedures The stratum corneum moisture content improvement rate was determined using a German CK skin moisture meter to measure the initial and 28-day stratum corneum moisture content of the test sites, and the moisture content improvement rate was calculated.

[0071] The rate of increase in stratum corneum moisture content = (Moisture content after test - Moisture content before test) / Moisture content before test × 100% Transdermal water loss (TEWL) reduction rate (barrier repair) was measured using the German CK skin TEWL tester to determine the initial and 28-day transdermal water loss values ​​and calculate the TEWL reduction rate. The higher the reduction rate, the stronger the skin barrier repair.

[0072] TEWL decline rate = (Post-test TEWL value - Pre-test TEWL value) / Pre-test TEWL value × 100% The soothing and repairing test results of the soothing and repairing compositions obtained in Examples 1-12 and Comparative Examples 1-6 are shown in Table 3.

[0073] Table 3. Soothing and repairing effects of the soothing and repairing compositions obtained in Examples 1-12 and Comparative Examples 1-6

[0074] The above description is merely an embodiment of the present invention and does not limit the scope of patent protection. Any non-substantial changes or substitutions made by those skilled in the art based on the present invention will still fall within the scope of patent protection.

Claims

1. A soothing repair composition characterized in that, It contains gentian root extract, forsythia extract and butylene glycol.

2. The soothing repair composition of claim 1, wherein The mass fractions of each component are: Gentian root extract 8-15 parts, Forsythia extract 12-24 parts, Butylene glycol 5-10 parts.

3. Process for the preparation of a soothing repair composition according to any one of claims 1 to 2, characterized in that, Includes the following steps: S1. Compound enzyme hydrolysis: Add compound enzyme to gentian root and forsythia respectively, and carry out enzymatic hydrolysis extraction at 37~39℃ to obtain gentian root suspension and forsythia suspension. S2. Three-phase system extraction: PEG-400 and ionic liquid were added to the gentian root suspension and the forsythia suspension respectively, and stirred evenly to form a water-PEG-400-ionic liquid three-phase extraction system. Extraction was carried out at 25~32℃, and after concentration, gentian root concentrated extract and forsythia concentrated extract were obtained. The ionic liquid is 1-butyl-3-methylimidazolium tetrafluoroborate; S3. Low-temperature directional fusion: Add concentrated extracts of gentian root and forsythia root in proportion, then add butylene glycol, stir evenly, let stand at 1~10℃ to fuse, and filter to obtain a soothing and repairing composition.

4. The method of making the soothing repair composition of claim 2, wherein, The compound enzyme added to the S1 dragon root contains cellulase and xylanase, while the compound enzyme added to the forsythia contains pectinase and hemicellulase.

5. The method of making the soothing repair composition of claim 2, wherein, The compound enzyme added to the S1 dragon root contains cellulase and xylanase, with a mass ratio of (2.5~3.5):

1.

6. The method of making the soothing repair composition of claim 2, wherein, The compound enzyme added to the S1 Forsythia contains pectinase and hemicellulase, with a mass ratio of (1.5~2.5):

1.

7. The method of making the soothing repair composition of claim 2, wherein, The S1 enzymatic extraction time is 2-3 hours.

8. The method of making the soothing repair composition of claim 2, wherein, The S2 gentian root suspension or forsythia suspension contains PEG-400 at a mass of 28%-32% of the suspension mass and ionic liquid at a mass of 4%-6% of the suspension mass.

9. The method of making the soothing repair composition of claim 2, wherein, The S2 extraction process involves applying an ultrasonic-microwave process, specifically: ultrasonic power of 180~220W and ultrasonic temperature of 25~30℃, followed by microwave power of 90~110W and microwave temperature of 28~32℃, with a total extraction time of 15~25min.

10. The use of the soothing and repairing composition according to any one of claims 1 to 2 in the field of skin care.

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