An antioxidant and stabilizing composition containing purslane extract and its application

Abstract

This invention relates to the field of cosmetic technology, and more particularly to an antioxidant and stabilizing composition containing purslane extract and its application. The composition of this invention comprises the following components in parts by weight: 1.0–10.0 parts purslane extract, 1.0–10.0 parts fermented mung bean sprout extract, 0.1–5.0 parts Inonotus obliquus polysaccharide, and 0.01–1.0 parts ergothioneine. This invention uses purslane extract as a soothing and stabilizing base, combined with fermented mung bean sprout extract, Inonotus obliquus polysaccharide, and ergothioneine, to form an antioxidant and stabilizing care system of "soothing repair – fermentation antioxidant – polysaccharide stabilization – cellular antioxidant protection," which can improve dullness, roughness, fatigue, redness, burning, oxidative stress, and unstable skin conditions. Experimental results show that the composition of the present invention can reduce oxidative stress levels, improve antioxidant-related indicators, and improve skin dullness, roughness, and decreased stability caused by oxidative stress. It has good gentleness and stability and can be prepared into cosmetic formulations such as serums, lotions, gels, toners, sprays, masks, emulsions, and creams, making it suitable for industrial production and daily skincare use.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and more particularly to an antioxidant and stabilizing composition containing purslane extract and its application. Background Technology

[0002] With the fast pace of modern life, factors such as staying up late, prolonged exposure to electronic screens, environmental pollution, UV radiation, irregular sleep patterns, emotional stress, and prolonged makeup use are becoming increasingly common. Skin is constantly under combined oxidative stress and external stimuli, easily leading to oxidative stress-related skin problems such as increased oxidative stress, decreased barrier stability, dull skin tone, roughness, fatigue, redness, burning sensation, dryness, and tightness. These problems are not caused by a single lifestyle scenario, but rather are a complex skin condition resulting from the combined effects of multiple external stimuli and internal stress.

[0003] Currently, most antioxidant cosmetics on the market mainly consist of single antioxidants or vitamins. While they can improve oxidative stress to some extent, some highly active antioxidants may be irritating to skin with a fragile barrier or sensitive skin, and long-term tolerance needs to be improved. Simple soothing products, although gentler, are insufficient in improving dullness, roughness, fatigue, and decreased skin stability caused by oxidative stress; simple moisturizing products, on the other hand, cannot address complex skin problems from multiple perspectives, including oxidative stress, soothing repair, and skin stabilization.

[0004] Purslane extract has excellent soothing, repairing, and skin conditioning properties, making it suitable for treating redness, burning, stinging, dryness, and unstable skin conditions. Mung bean sprout fermentation extract contains polyphenols, polysaccharides, amino acids, and small-molecule fermentation metabolites, making it suitable for antioxidant and stabilizing skin affected by oxidative stress. Inonotus obliquus polysaccharide has nourishing and antioxidant properties, improving rough and tired skin. Ergothioneine, as a highly effective antioxidant, can help reduce oxidative stress and improve skin stability.

[0005] In existing technologies, there is no known formula that combines purslane extract, fermented mung bean sprout extract, Inonotus obliquus polysaccharide, and ergothioneine in a specific ratio to achieve a mild herbal fermentation compound with three combined effects of anti-oxidation, soothing, and skin stabilization. Therefore, developing a scientifically formulated, mild, and stable anti-oxidative and stabilizing composition that can improve dullness, roughness, fatigue, redness, burning sensation, oxidative stress, and unstable skin conditions has significant practical application value. Summary of the Invention

[0006] In view of this, the purpose of the present invention is to provide an antioxidant and stabilizing composition containing purslane extract and its application.

[0007] To achieve the above-mentioned objectives, the present invention provides the following technical solution:

[0008] This invention provides an antioxidant and stabilizing composition containing purslane extract, comprising the following components in parts by weight: 1.0-10.0 parts of purslane extract, 1.0-10.0 parts of fermented mung bean sprout extract, 0.1-5.0 parts of Inonotus obliquus polysaccharide, and 0.01-1.0 parts of ergothioneine.

[0009] Preferably, the components include the following parts by weight: 2.0 to 6.0 parts of purslane extract, 2.0 to 6.0 parts of mung bean sprout fermentation extract, 0.5 to 3.0 parts of Inonotus obliquus polysaccharide, and 0.02 to 0.2 parts of ergothioneine.

[0010] Further preferred components include the following parts by weight: 4.0 parts of purslane extract, 4.0 parts of mung bean sprout fermentation extract, 1.5 parts of Inonotus obliquus polysaccharide, and 0.2 parts of ergothioneine.

[0011] Preferably, the purslane extract is one of the following: purslane water extract, purslane alcohol extract, purslane hydro-alcohol extract, purslane enzymatic hydrolysis extract, and purslane fermentation extract; the mung bean sprout fermentation extract is obtained by inoculating mung bean sprouts with lactic acid bacteria, yeast, bifidobacteria, or a combination of bacteria for fermentation, followed by water extraction, hydro-alcohol extraction, or enzymatic hydrolysis.

[0012] Preferably, the total flavonoid content in the purslane extract is ≥1.0%; and the total polyphenol content in the mung bean sprout fermentation extract is ≥0.5% and the total polysaccharide content is ≥2.0%.

[0013] Preferably, the beech polysaccharide is one of beech extract, beech polysaccharide extract, and beech fermentation product, and the polysaccharide content in the beech polysaccharide is ≥10%.

[0014] Preferably, the composition further includes one or more of carnosine, ectoine, trehalose, and panthenol, and the total weight of carnosine, ectoine, trehalose, and panthenol is 0.1 to 5.0 parts.

[0015] Preferably, the composition further includes one or more of vitamin E, coenzyme Q10, ferulic acid, and sodium hyaluronate, and the total weight of vitamin E, coenzyme Q10, ferulic acid, and sodium hyaluronate is 0.01 to 3.0 parts.

[0016] Preferably, the composition further includes a cosmetically acceptable matrix, which includes one or more of water, glycerin, butylene glycol, 1,2-pentanediol, thickener, preservative aid, and pH adjuster, and the pH value of the composition is 5.0 to 7.0.

[0017] Preferably, the dosage form of the composition is one of the following: serum, essence lotion, gel, toner, spray, mask, emulsion, and cream.

[0018] The present invention also provides the application of the antioxidant and stabilizing composition in the preparation of cosmetics, which have the effects of improving dull skin, roughness, fatigue, redness, burning, oxidative stress and unstable skin condition.

[0019] Beneficial effects

[0020] This invention uses purslane extract, fermented mung bean sprout extract, birch polysaccharide, and ergothionein as its core active ingredients. The scientifically formulated and clearly layered composition forms an antioxidant and stabilizing skincare system that combines "soothing and stabilizing - fermentation and anti-oxidation - polysaccharide nourishment - cellular antioxidant protection." This system can address multiple skin problems such as dullness, roughness, fatigue, redness, burning, oxidative stress, and unstable skin conditions.

[0021] In this invention, purslane extract serves as a soothing and repairing base, improving discomfort such as redness, burning, stinging, and tightness caused by external stimuli and oxidative stress. Mung bean sprout fermentation extract, rich in polyphenols, polysaccharides, amino acids, and small-molecule fermentation metabolites, enhances the skin's antioxidant and stabilizing abilities. Inonotus obliquus polysaccharide possesses nourishing and antioxidant properties, improving roughness and fatigue. Ergothioneine, as an antioxidant, further reduces oxidative stress levels. The synergistic effect of these four ingredients significantly enhances the antioxidant and stabilizing effects compared to single-ingredient or missing-component formulations.

[0022] Experimental results showed that the absence of purslane extract, mung bean sprout fermentation extract, birch polysaccharide, or ergothioneine significantly reduced the composition's effectiveness in improving reactive oxygen species levels, antioxidant enzyme activity, and soothing-related indicators. This indicates that the four core components are not simply additive, but rather have a synergistic effect in stabilizing and protecting the skin in the context of oxidative stress.

[0023] The composition of this invention has good mildness and is suitable for daily care of dull and rough skin, red and unstable skin, and skin related to oxidative stress. It can also be prepared into various dosage forms such as serum, essence lotion, gel, toner, spray, mask, emulsion, and cream. The preparation process is simple, the stability is good, and it is suitable for industrial production. Detailed Implementation

[0024] This invention provides an antioxidant and stabilizing composition containing purslane extract, comprising the following components in parts by weight: 1.0-10.0 parts of purslane extract, 1.0-10.0 parts of fermented mung bean sprout extract, 0.1-5.0 parts of Inonotus obliquus polysaccharide, and 0.01-1.0 parts of ergothioneine.

[0025] Purslane extract: 1.0 to 10.0 parts, preferably 2.0 to 6.0 parts, more preferably 4.0 parts;

[0026] The fermented mung bean sprout extract contains 1.0 to 10.0 parts, preferably 2.0 to 6.0 parts, and more preferably 4.0 parts;

[0027] The polysaccharide content of Inonotus obliquus is 0.1 to 5.0 parts, preferably 0.5 to 3.0 parts, and more preferably 1.5 parts;

[0028] Ergothioneine: 0.01 to 1.0 parts, preferably 0.02 to 0.2 parts, more preferably 0.2 parts.

[0029] In this invention, the purslane extract can be prepared by the following method: dried whole purslane herb is taken, pulverized, and 8-12 times the amount of water or 50-80% ethanol is added. The mixture is refluxed and extracted 1-3 times, each time for 1-2 hours. The extract is filtered, the filtrates are combined, concentrated under reduced pressure, and dried to obtain the final product. The total flavonoid content in the purslane extract is preferably ≥1.0%. The purslane extract has excellent soothing, repairing, and skin conditioning effects, and can improve skin redness, burning, stinging, and barrier instability caused by oxidative stress and external stimuli.

[0030] In this invention, the fermented mung bean sprout extract can be prepared by the following method: Fresh mung bean sprouts are taken, washed, crushed, and then mixed with 1 to 3 times their weight of purified water to make a slurry. Lactic acid bacteria, yeast, bifidobacteria, or a combination of bacteria are inoculated into the slurry at a rate of 0.5 to 2.0% of the mung bean sprout slurry weight. Fermentation is carried out at 30 to 37°C for 24 to 72 hours, preferably 48 hours. After fermentation, sterilization, filtration, concentration, and drying are performed to obtain the fermented mung bean sprout extract. The total polyphenol content in the fermented mung bean sprout extract is preferably ≥0.5%, and the total polysaccharide content is preferably ≥2.0%. The fermented mung bean sprout extract can provide polyphenols, polysaccharides, amino acids, and small molecule fermentation metabolites, making it suitable for antioxidant and stabilizing skin care related to oxidative stress.

[0031] In this invention, the *Inonotus obliquus* polysaccharide is one of *Inonotus obliquus* extract, *Inonotus obliquus* polysaccharide extract, and *Inonotus obliquus* fermentation product, and the polysaccharide content in the *Inonotus obliquus* polysaccharide is preferably ≥10%. *Inonotus obliquus* polysaccharide can enhance skin nourishment and antioxidant care effects, and improve roughness, dullness, and fatigue of skin related to oxidative stress.

[0032] In this invention, ergothioneine can help reduce oxidative stress and improve skin stability. When combined with purslane extract, mung bean sprout fermented extract and Inonotus obliquus polysaccharide, it can form a multi-layered antioxidant and skin-stabilizing care system that ranges from soothing, fermentation antioxidation, polysaccharide nourishment to antioxidant protection.

[0033] In this invention, the composition may further include one or more of carnosine, ectoine, trehalose, and panthenol, and the total weight of carnosine, ectoine, trehalose, and panthenol is preferably 0.1 to 5.0 parts; carnosine is used to assist in anti-oxidation and improve roughness, ectoine is used to enhance the skin's stress resistance, trehalose is used to improve dryness and enhance cell protection, and panthenol is used to soothe, repair, and improve the stability of the skin barrier.

[0034] In this invention, the composition may further include one or more of vitamin E, coenzyme Q10, ferulic acid, and sodium hyaluronate, and the total weight of vitamin E, coenzyme Q10, ferulic acid, and sodium hyaluronate is preferably 0.01 to 3.0 parts, in order to further improve antioxidant, moisturizing, and skin suppleness.

[0035] The compositions of this invention can be supplemented with cosmetically acceptable bases, including but not limited to water, glycerin, butylene glycol, 1,2-pentanediol, thickeners, preservatives, pH adjusters, emulsifiers, and oil bases, depending on the specific dosage form. The pH value of the composition is preferably 5.0–7.0. The compositions of this invention can be prepared into dosage forms such as serums, lotions, gels, toners, sprays, masks, emulsions, and creams.

[0036] The compositions of this invention can be prepared using conventional processes in the cosmetics field. For aqueous systems such as serums, gels, toners, or sprays, purified water, glycerin, butylene glycol, and other aqueous matrix components can be added to a mixing tank under conditions that meet the hygiene requirements for cosmetic production. Purslane extract, fermented mung bean sprout extract, Inonotus obliquus polysaccharide, ergothioneine, and other water-soluble components are then added sequentially. After stirring evenly, thickeners, preservatives, and pH adjusters are added to adjust the pH to 5.0–7.0. For emulsified systems such as lotions, creams, and serums, aqueous and oil phases can be prepared separately. The aqueous and oil phases are mixed and emulsified at a suitable temperature, homogenized, and then cooled to below 45°C. The core active components and other heat-sensitive components are then added, stirred evenly, and the pH is adjusted to 5.0–7.0.

[0037] Example

[0038] Example 1: Antioxidant and Stabilizing Essence

[0039] Table 1. Composition of the Antioxidant and Stabilizing Essence in Example 1

[0040] Components weight Purslane extract 4.0 mung bean sprout fermentation extract 4.0 Inonotus obliquus polysaccharide 1.5 Ergothioneine 0.20 Carnosine 0.30 glycerin 5.0 Butylene glycol 4.0 Sodium hyaluronate 0.10 1,2-Pentanediol 0.50 p-Hydroxyacetophenone 0.30 Carbomer 0.15 Arginine 0.12 Purified water Supplement to 100

[0041] Preparation method: Under conditions that meet the hygiene requirements for cosmetic production, purified water, glycerin, butylene glycol and other aqueous raw materials are added to a mixing pot and stirred evenly. Then, purslane extract, mung bean sprout fermentation extract, birch polysaccharide, ergothioneine and other water-soluble components are added, followed by thickener and preservative adjuvant. The mixture is stirred evenly and the pH is adjusted to 5.0-7.0 to obtain the final product.

[0042] Example 2: Antioxidant and Stabilizing Essence Lotion

[0043] Table 2 Composition of Antioxidant and Stabilizing Essence Lotion in Example 2

[0044] Components weight Purslane extract 5.0 mung bean sprout fermentation extract 3.0 Inonotus obliquus polysaccharide 2.0 Ergothioneine 0.10 Ikdoin 0.60 Panthenol 1.0 glycerin 5.0 Butylene glycol 3.0 squalane 3.0 Caprylic / Capric Triglyceride 3.0 Cetearyl alcohol 1.2 Glyceryl stearate 1.0 1,2-Pentanediol 0.50 p-Hydroxyacetophenone 0.30 Purified water Supplement to 100

[0045] Preparation method: Under conditions that meet the hygiene requirements for cosmetic production, an aqueous phase and an oil phase are prepared separately. The aqueous phase and the oil phase are mixed and emulsified at a suitable temperature, homogenized, and then cooled to below 45°C. Purslane extract, mung bean sprout fermentation extract, Inonotus obliquus polysaccharide, ergothioneine, and other heat-sensitive components are then added, stirred evenly, and the pH is adjusted to 5.0-7.0 to obtain the final product.

[0046] Example 3 Antioxidant Stabilizing Gel

[0047] Table 3 Composition of Antioxidant Stabilizing Gel in Example 3

[0048] Components weight Purslane extract 3.0 mung bean sprout fermentation extract 5.0 Inonotus obliquus polysaccharide 1.0 Ergothioneine 0.15 Trehalose 2.0 glycerin 4.0 Butylene glycol 4.0 Sodium hyaluronate 0.08 Hydroxyethyl cellulose 0.25 1,2-Pentanediol 0.50 p-Hydroxyacetophenone 0.30 Purified water Supplement to 100

[0049] Preparation method: Under conditions that meet the hygiene requirements for cosmetic production, purified water, humectant and thickener are added to a mixing pot and dispersed evenly. Then, purslane extract, mung bean sprout fermentation extract, birch polysaccharide, ergothioneine and other functional components are added, stirred evenly, and the pH is adjusted to 5.0-7.0 to obtain the final product.

[0050] Example 4: Antioxidant and Stabilizing Facial Mask Essence

[0051] Table 4. Composition of the Antioxidant and Stabilizing Mask Essence in Example 4

[0052] Components weight Purslane extract 4.0 mung bean sprout fermentation extract 4.0 Inonotus obliquus polysaccharide 1.5 Ergothioneine 0.12 Panthenol 1.0 Trehalose 1.5 glycerin 5.0 Butylene glycol 4.0 Sodium hyaluronate 0.10 β-glucan 0.30 1,2-Pentanediol 0.50 p-Hydroxyacetophenone 0.30 Hydroxyethyl cellulose 0.18 Purified water Supplement to 100

[0053] Preparation method: Under conditions that meet the hygiene requirements for cosmetic production, purified water, glycerin, butylene glycol and film-forming or thickening components are mixed and dispersed, and purslane extract, mung bean sprout fermentation extract, Inonotus obliquus polysaccharide, ergothioneine and other water-soluble components are added. The mixture is stirred evenly and the pH is adjusted to 5.0-7.0 to obtain the final product.

[0054] Example 5: Antioxidant and Stabilizing Face Cream

[0055] Table 5. Composition of the Antioxidant and Stabilizing Face Cream in Example 5

[0056] Components weight Purslane extract 4.0 mung bean sprout fermentation extract 3.0 Inonotus obliquus polysaccharide 2.0 Ergothioneine 0.10 Coenzyme Q10 0.05 Vitamin E 0.20 glycerin 5.0 Butylene glycol 3.0 squalane 4.0 Caprylic / Capric Triglyceride 4.0 Cetearyl alcohol 1.5 Glyceryl stearate 1.2 polydimethylsiloxane 1.0 1,2-Hexanediol 0.50 p-Hydroxyacetophenone 0.30 Purified water Supplement to 100

[0057] Preparation method: Under conditions that meet the hygiene requirements for cosmetic production, an aqueous phase and an oil phase are prepared separately. The aqueous phase and the oil phase are mixed and emulsified at a suitable temperature, homogenized, and then cooled to below 45°C. Purslane extract, mung bean sprout fermentation extract, Inonotus obliquus polysaccharide, ergothioneine, and other heat-sensitive components are then added, stirred evenly, and the pH is adjusted to 5.0-7.0 to obtain the final product.

[0058] Comparative Example

[0059] The comparative examples were designed using a single-factor missing design based on Example 1. Except for the adjustments shown in the table, the other components and preparation methods were the same as in Example 1.

[0060] Table 6. Instructions for Adjusting the Comparative Formulation

[0061] Group Adjustment content Comparative Example 1 No purslane extract added, purified water used to make up for the deficiency. Comparative Example 2 No added mung bean sprout fermentation extract, purified water is used to supplement Comparative Example 3 No added birch polysaccharide, purified water replenished Comparative Example 4 No ergothioneine added, purified water supplemented. Comparative Example 5 Only purslane extract was added; other core active ingredients were not added, and purified water was used to make up the difference. Comparative Example 6 Only ergothioneine was added; other core active ingredients were not added, and purified water was used to make up the difference. Comparative Example 7 The blank matrix group does not contain purslane extract, mung bean sprout fermentation extract, birch polysaccharide, or ergothioneine, and is supplemented with purified water.

[0062] Experimental Example 1: In vitro antioxidant evaluation

[0063] The antioxidant effects of each group of samples were evaluated using an oxidative stress cell model. Human skin keratinocytes were cultured routinely. After the cells reached good growth status, they were treated with the sample dilutions of Examples 1-5 and Comparative Examples 1-7 for 24 hours, respectively, followed by the induction of oxidative stress with hydrogen peroxide. The control group consisted of cells not stimulated with hydrogen peroxide. The relative level of intracellular reactive oxygen species was detected using a fluorescent probe method, with the model group representing 100%.

[0064] Table 7. In vitro antioxidant evaluation results of each group of samples

[0065] Group Relative level of reactive oxygen species / % Blank group 40.8±3.0** Model group 100.0±5.6 Example 1 43.6±3.2** Example 2 47.8±3.5** Example 3 45.1±3.4** Example 4 46.3±3.5** Example 5 48.5±3.7** Comparative Example 1 66.9±4.6* Comparative Example 2 70.4±4.8* Comparative Example 3 73.2±5.0* Comparative Example 4 68.7±4.7* Comparative Example 5 78.6±5.3* Comparative Example 6 75.1±5.1* Comparative Example 7 89.4±5.8

[0066] Note: Compared with the model group, *P<0.05, **P<0.01.

[0067] As shown in Table 7, compared with the model group, Examples 1-5 all significantly reduced the relative level of intracellular reactive oxygen species (ROS), with Example 1 showing the best effect. The antioxidant effect decreased in Comparative Examples 1-4 after the absence of any core component. The relative ROS level of Example 1 was significantly lower than that of Comparative Example 5 (containing only purslane extract) and Comparative Example 6 (containing only ergothioneine), indicating that the combination of purslane extract, mung bean sprout fermentation extract, Inonotus obliquus polysaccharide, and ergothioneine is not a simple additive effect, but rather exhibits a synergistic effect in reducing oxidative stress.

[0068] Experiment Example 2: Evaluation of Antioxidant Related Indicators

[0069] An oxidative stress model was established using ultraviolet stimulation to evaluate the effects of the composition on glutathione peroxidase activity, superoxide dismutase activity, and malondialdehyde (MDA) levels. Relevant indicators were measured after treatment in each group, with the model group representing 100%.

[0070] Table 8 Evaluation results of antioxidant-related indicators for each group of samples

[0071] Group Glutathione peroxidase activity / % Superoxide dismutase activity / % relative level of malondialdehyde (MDA) / % Blank group 155.2±8.4** 160.4±8.8** 48.6±4.0** Model group 100.0±6.2 100.0±6.0 100.0±6.5 Example 1 148.6±7.8** 151.7±8.0** 52.4±4.2** Example 2 139.5±7.2** 143.2±7.5** 58.6±4.5** Example 3 142.8±7.4** 146.5±7.6** 55.9±4.4** Example 4 145.1±7.6** 149.4±7.8** 54.7±4.3** Example 5 137.4±7.0** 140.8±7.2** 60.3±4.8** Comparative Example 1 121.7±6.5* 124.3±6.6* 74.6±5.3* Comparative Example 2 118.9±6.2* 120.8±6.4* 78.5±5.5* Comparative Example 3 116.4±6.0* 119.2±6.2* 80.1±5.7* Comparative Example 4 123.5±6.7* 126.1±6.8* 73.2±5.2*

[0072] Note: Compared with the model group, *P<0.05, **P<0.01.

[0073] As shown in Table 8, the Example group significantly increased the activity of antioxidant-related enzymes and reduced the relative level of malondialdehyde (MDA), indicating that the composition of the present invention can effectively improve oxidative stress. Compared with Comparative Examples 1-4, Examples 1-5 showed more significant improvements in glutathione peroxidase activity, superoxide dismutase activity, and MDA levels, indicating that the antioxidant stabilization effect decreased after the absence of any core component, further demonstrating the synergistic effect of the composition of the present invention.

[0074] Experiment Example 3: Evaluation of Relief and Stability

[0075] The soothing and stabilizing effects of the composition were evaluated using a stimulation-induced keratinocyte model. Cells were divided into a control group, a model group, an example group, and a control group. After treatment, the relative expression levels of interleukin-6 and tumor necrosis factor were measured, with the model group representing 100%.

[0076] Table 9 Evaluation results of soothing and stabilizing effects for each group of samples

[0077] Group Relative expression level of interleukin-6 / % Relative expression level of tumor necrosis factor / % Blank group 39.4±3.3** 42.0±3.5** Model group 100.0±6.3 100.0±6.6 Example 1 44.8±3.7** 47.6±3.9** Example 2 49.5±4.0** 52.1±4.2** Example 3 47.3±3.9** 50.4±4.0** Example 4 48.2±4.0** 51.5±4.1** Example 5 51.1±4.2** 54.2±4.4** Comparative Example 1 67.5±4.8* 70.2±5.1* Comparative Example 2 72.1±5.2* 74.5±5.4* Comparative Example 3 69.8±5.0* 72.4±5.2* Comparative Example 4 66.9±4.7* 69.1±5.0*

[0078] Note: Compared with the model group, *P<0.05, **P<0.01.

[0079] As shown in Table 9, Examples 1 to 5 can significantly reduce the relative expression levels of relevant factors in the stimulation model, indicating that the composition of the present invention has a good soothing and stabilizing effect.

[0080] Experiment Example 4: Evaluation of Human Use

[0081] Sixty participants aged 22–40 years with oxidative stress-related skin conditions were randomly divided into four groups: Example 1, Example 3, Comparative Example 1, and Comparative Example 2, with 15 participants in each group. Inclusion criteria included participants experiencing dullness, roughness, fatigue, redness, or skin instability within the past month due to factors such as prolonged exposure to electronic screens, environmental pollution, UV radiation, irregular sleep patterns, emotional stress, or prolonged makeup use. Exclusion criteria included participants who were pregnant, breastfeeding, had a history of skin allergies, had used potent skincare products within the past month, had undergone cosmetic procedures within the past three months, or had significant skin diseases during the trial period. Participants used the corresponding sample twice daily, morning and evening, for eight consecutive weeks. Improvements in dullness, roughness, redness, fatigue, and skin stability were evaluated. The stability improvement rate was defined as the proportion of participants who reported less redness, dryness, stinging, burning, or tightness compared to before use, calculated using a 0–10 scale based on changes in scores before and after use.

[0082] Table 10. Human User Evaluation Results (Oxidative Stress-Related Skin)

[0083] Group Dullness improvement rate / % Roughness improvement rate / % Redness improvement rate / % Fatigue improvement rate / % Stability improvement rate / % Example 1 45.6±4.6**#△ 48.3±4.8**#△ 50.7±5.0**#△ 52.4±5.2**#△ 55.2±5.3**#△ Example 3 41.8±4.3**#△ 44.5±4.6**#△ 47.2±4.8**#△ 49.6±5.0**#△ 51.4±5.1**#△ Comparative Example 1 27.4±3.7* 29.1±3.8* 31.2±4.0* 32.6±4.1* 34.5±4.2* Comparative Example 2 25.8±3.5* 27.6±3.6* 29.4±3.8* 31.1±3.9* 32.8±4.0*

[0084] Note: Compared with before use, *P<0.05, **P<0.01; compared with Comparative Example 1, #P<0.05; compared with Comparative Example 2, △P<0.05.

[0085] As shown in Table 10, Examples 1 and 3 are superior to the comparative group in improving skin dullness, roughness, redness, fatigue and skin stability related to oxidative stress, indicating that the composition of the present invention has good antioxidant and skin-stabilizing effects.

[0086] Experimental Example 5: Safety Evaluation of Human Patches

[0087] Thirty healthy subjects were selected, and samples from Examples 1 to 5 were subjected to closed patch tests. The patches were removed after 24 hours, and skin reactions were observed at 0.5 hours, 24 hours, and 48 hours. Erythema, edema, papules, itching, and other conditions were recorded.

[0088] Table 11 Safety Evaluation Results of Human Patches

[0089] sample Number of subjects Number of negative cases Number of people with mild reactions The number of people who clearly reacted Safety assessment Example 1 30 30 0 0 No obvious irritation Example 2 30 30 0 0 No obvious irritation Example 3 30 29 1 0 mild Example 4 30 30 0 0 No obvious irritation Example 5 30 30 0 0 No obvious irritation

[0090] As shown in Table 11, no obvious adverse reactions were observed in the human patch test of Examples 1 to 5, indicating that the composition of the present invention is mild and suitable for daily skin care.

[0091] Stability test

[0092] Stability studies were conducted on samples from Examples 1, 2, and 3. Long-term stability was tested at room temperature in the dark, with samples taken at 0, 3, 6, 12, 18, 24, and 36 months. Accelerated stability was tested at 40℃±2℃ and 75%±5% relative humidity for 6 months. High-low temperature cycling was performed by alternating cycles at -5℃ and 45℃ three times. Centrifugal stability was tested by centrifugation at 3000 rpm for 15 minutes, observing for stratification, precipitation, significant discoloration, or abnormal odor. Tests included appearance, color, odor, pH, centrifugal stability, retention rate of core active ingredients, and microbial limits. Total flavonoids from purslane and total polyphenols from mung bean sprout fermentation extract were detected using ultraviolet spectrophotometry; total polysaccharides from mung bean sprout fermentation extract and polysaccharides from Inonotus obliquus were detected using the phenol-sulfuric acid method; and ergothionein was detected using high-performance liquid chromatography.

[0093] Table 12 Long-term stability, appearance, and pH evaluation results

[0094] Time / Month Example 1 Appearance Example 1 pH Example 2 Appearance Example 2 pH Example 3 Appearance Example 3 pH 0 Even and smooth texture, no layering, no odor 6.10 A uniform emulsion, without separation or odor. 6.05 The condensation is uniform, without layering or odor. 6.12 3 Even and smooth texture, no layering, no odor 6.08 A uniform emulsion, without separation or odor. 6.02 The condensation is uniform, without layering or odor. 6.10 6 Even and smooth texture, no layering, no odor 6.05 A uniform emulsion, without separation or odor. 6.00 The condensation is uniform, without layering or odor. 6.08 12 Even and smooth texture, no layering, no odor 6.02 A uniform emulsion, without separation or odor. 5.97 The condensation is uniform, without layering or odor. 6.05 18 Even and smooth texture, no layering, no odor 5.99 A uniform emulsion, without separation or odor. 5.95 The condensation is uniform, without layering or odor. 6.02 24 Even and smooth texture, no layering, no odor 5.96 A uniform emulsion, without separation or odor. 5.92 The condensation is uniform, without layering or odor. 5.98 36 Even and smooth texture, no layering, no discoloration, no odor 5.92 A uniform emulsion, without separation, discoloration, or off-odor. 5.88 The condensation is uniform, without layering, discoloration, or odor. 5.94

[0095] Note: During the long-term stability study, the microbial limits of the samples at each time point met the relevant quality requirements for cosmetics.

[0096] Table 13 Results of core active ingredient retention rate determination

[0097] Time / Month Purslane total flavonoid retention rate / % Total polyphenol retention rate of mung bean sprouts / % Total polysaccharide retention rate of mung bean sprouts / % Chaga polysaccharide retention rate / % Ergothioneine retention rate / % 0 100.0 100.0 100.0 100.0 100.0 3 98.5 98.2 98.8 99.0 99.1 6 97.6 97.2 98.1 98.4 98.5 12 96.1 95.8 96.9 97.1 97.4 18 94.8 94.2 95.6 96.0 96.2 24 93.4 92.7 94.2 94.8 95.0 36 91.2 90.6 92.5 93.4 94.0

[0098] Table 14 Accelerated stability and high and low temperature cycling evaluation results

[0099] sample Accelerate the appearance by 6 months Accelerate pH for 6 months Accelerate 6-month core activity retention rate / % High and low temperature cycling results Microbial limits Example 1 Even and smooth texture, no layering, no odor 5.94 94.1 No layering, no precipitation, no obvious discoloration Meets requirements Example 2 A uniform emulsion, without separation or odor. 5.90 93.6 No layering, no precipitation, no obvious discoloration Meets requirements Example 3 The condensation is uniform, without layering or odor. 5.96 94.3 No layering, no precipitation, no obvious discoloration Meets requirements

[0100] Note: "Meets requirements" for microbial limits means that the total number of colonies, total number of molds and yeasts, as well as thermotolerant coliforms, Staphylococcus aureus, Pseudomonas aeruginosa, and other indicators meet the relevant quality requirements for cosmetics.

[0101] As shown in Tables 12-14, after 36 months of long-term stability, 6 months of accelerated stability, and high and low temperature cycling tests, the samples of the present invention maintained good appearance, odor, pH value, and retention rate of core active ingredients. At the same time, the microbial limits of the samples at each time point met the relevant requirements for cosmetics, and no abnormal microbial growth was observed, indicating that the composition of the present invention has good long-term stability and feasibility for industrial application.

[0102] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. An antioxidant and stabilizing composition containing purslane extract, characterized in that, It includes the following components in parts by weight: purslane extract 1.0 to 10.0 parts, mung bean sprout fermentation extract 1.0 to 10.0 parts, Inonotus obliquus polysaccharide 0.1 to 5.0 parts, and ergothioneine 0.01 to 1.0 parts.

2. The antioxidant and stabilizing composition according to claim 1, characterized in that, It includes the following components in parts by weight: purslane extract 2.0–6.0 parts, mung bean sprout fermentation extract 2.0–6.0 parts, Inonotus obliquus polysaccharide 0.5–3.0 parts, and ergothioneine 0.02–0.2 parts.

3. The antioxidant and stabilizing composition according to claim 1 or 2, characterized in that, The purslane extract is one of the following: purslane water extract, purslane alcohol extract, purslane hydro-alcohol extract, purslane enzymatic hydrolysis extract, and purslane fermentation extract; the mung bean sprout fermentation extract is obtained by fermenting mung bean sprouts with lactic acid bacteria, yeast, bifidobacteria, or a combination of bacteria, followed by water extraction, hydro-alcohol extraction, or enzymatic hydrolysis.

4. The antioxidant and stabilizing composition according to any one of claims 1 to 3, characterized in that, The purslane extract contains ≥1.0% total flavonoids; the mung bean sprout fermentation extract contains ≥0.5% total polyphenols and ≥2.0% total polysaccharides.

5. The antioxidant and stabilizing composition according to any one of claims 1 to 4, characterized in that, The betulia polysaccharide is one of betulia extract, betulia polysaccharide extract, and betulia fermentation product, and the polysaccharide content of the betulia polysaccharide is ≥10%.

6. The antioxidant and stabilizing composition according to any one of claims 1 to 5, characterized in that, The composition further includes one or more of carnosine, ectoine, trehalose, and panthenol, and the total weight of carnosine, ectoine, trehalose, and panthenol is 0.1 to 5.0 parts.

7. The antioxidant and stabilizing composition according to any one of claims 1 to 6, characterized in that, The composition further includes one or more of vitamin E, coenzyme Q10, ferulic acid, and sodium hyaluronate, and the total weight of vitamin E, coenzyme Q10, ferulic acid, and sodium hyaluronate is 0.01 to 3.0 parts.

8. The antioxidant and stabilizing composition according to any one of claims 1 to 7, characterized in that, The composition further includes a cosmetically acceptable matrix, which includes one or more of water, glycerin, butylene glycol, 1,2-pentanediol, thickener, preservative aid, and pH adjuster, and the composition has a pH value of 5.0 to 7.

0.

9. The antioxidant and stabilizing composition according to any one of claims 1 to 8, characterized in that, The dosage form of the composition is one of the following: serum, essence lotion, gel, toner, spray, mask, emulsion, and cream.

10. The use of the antioxidant and stabilizing composition according to any one of claims 1 to 9 in the preparation of cosmetics, wherein the cosmetics have the effect of improving dull skin, roughness, fatigue, redness, burning, oxidative stress and unstable skin condition.