A magnetic adsorbent for separating protein and pigment, a preparation method and application thereof

By preparing an ε-polylysine-modified magnetic adsorbent, the problem of simultaneous removal of proteins and pigments from shiitake mushroom polysaccharide extract was solved, achieving efficient and simple separation and purification, which is suitable for industrial production.

CN122298375APending Publication Date: 2026-06-30NORTHWEST A & F UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NORTHWEST A & F UNIV
Filing Date
2026-04-24
Publication Date
2026-06-30

AI Technical Summary

Technical Problem

Existing technologies are insufficient for efficiently and simultaneously removing proteins and pigments from shiitake mushroom polysaccharide extracts. Traditional methods are cumbersome, leave organic reagent residues, and are costly, making them difficult to apply on a large scale.

Method used

A magnetic adsorbent with a core of iron oxide coated with silica and modified to ε-polylysine is used to adsorb proteins and pigments through electrostatic and hydrogen bonding interactions. Combined with magnetic separation technology, the operation is simplified.

Benefits of technology

It achieves efficient adsorption of proteins and pigments in shiitake mushroom polysaccharide extract, simplifies the operation process, reduces costs, and is reusable, making it suitable for industrial production.

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Abstract

This invention discloses a magnetic adsorbent for separating proteins and pigments, its preparation method, and its application. First, ferrous and ferric salts are dissolved in a mixed solvent of water and alcohol, heated to 60–80°C, and an alkaline precipitant is added while stirring until a black precipitate is completely formed, yielding ferric oxide (Fe3O4). Next, the obtained ferric oxide is dispersed in an alcohol-water mixture, and ammonia and tetraethyl silicate are added. The mixture is stirred at room temperature for 2–5 hours to obtain silica-coated ferric oxide. Finally, ε-polylysine is dissolved in water, and the silica-coated ferric oxide is added. After ultrasonic dispersion, the mixture is stirred at 20–30°C for 10–24 hours. After washing and drying, an ε-polylysine-modified magnetic adsorbent is obtained. This invention is used for the purification of lentinan extract, specifically adsorbing proteins and pigments, and can be rapidly separated by an external magnetic field. It has the advantages of simple operation and high adsorption efficiency.
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