Methods and applications of heterologous overexpression of the ZjUBQ3 gene in jujube trees to increase grain length and thousand-grain weight in rice.

By cloning the ZjUBQ3 gene from jujube trees and heterologously overexpressing it in rice, the problem of difficult genetic transformation of jujube trees was solved, and the grain length and thousand-grain weight of rice were significantly increased, providing new gene resources and improvement strategies for rice breeding.

CN122303263APending Publication Date: 2026-06-30LUOYANG NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
LUOYANG NORMAL UNIV
Filing Date
2026-06-03
Publication Date
2026-06-30

AI Technical Summary

Technical Problem

Existing technologies make it difficult to effectively utilize the ZjUBQ3 gene from jujube trees for heterologous overexpression in rice to increase grain length and thousand-grain weight. Furthermore, genetic transformation of the woody plant jujube is difficult, and progress in gene function verification is slow.

Method used

The ZjUBQ3 gene was cloned from the jujube variety 'Dongzao', and the overexpression vector pCAMBIA1300-ZjUBQ3 was constructed. The gene was then transformed into rice using Agrobacterium-mediated transformation to achieve heterologous overexpression of the ZjUBQ3 gene in rice.

Benefits of technology

It significantly increases the grain length and thousand-grain weight of rice seeds, while the grain width does not change significantly, providing new gene resources for high-yield and high-quality rice breeding, verifying that ZjUBQ3 is a positive regulatory factor, and providing improvement strategies.

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Abstract

This invention discloses a method and application for heterologous overexpression of the ZjUBQ3 gene from jujube trees to increase grain length and thousand-grain weight in rice, relating to the field of plant genetic engineering. The coding sequence of the ZjUBQ3 gene was obtained from the jujube variety 'Dongzao' via PCR amplification. This sequence is 1146 bp in length, encoding 381 amino acids composed of five ubiquitin monomers linked together, and its nucleotide sequence is shown in SEQ ID NO:1. This sequence was constructed into an overexpression vector and transformed into the rice variety 'Zhonghua 11', achieving heterologous overexpression of ZjUBQ3 in rice. Experimental results showed that, compared with the wild type, the transgenic rice lines heterologously overexpressing ZjUBQ3 exhibited significantly increased grain length and thousand-grain weight, while grain width remained largely unchanged. This invention confirms that the ZjUBQ3 gene from jujube trees has a positive regulatory effect on seed size, providing a valuable new gene resource for molecular breeding of high-yield and high-quality rice.
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