Liquid chromatography apparatus and detection assembly therefor

Abstract

This invention relates to the field of medical devices and discloses a liquid chromatography analysis device and detection assembly. The liquid chromatography analysis device includes a sample delivery assembly, a sample preparation channel, a liquid flow delivery assembly, a chromatographic column, a detection assembly, and a controller. The detection assembly includes a light source, an optical shaping component, a flow meter, a light receiving component, and a first mounting component. The flow meter forms a detection channel. The first mounting component forms a mounting channel, which is used to mount at least the optical shaping component and the flow meter. The mounting channel has an arc-shaped mounting portion for mounting the optical shaping component and / or the flow meter. The central axis of the mounting portion, the central axis of the detection channel, and the optical axis of the optical shaping component are substantially coincident. This invention improves the installation and debugging efficiency of the optical shaping component and the flow meter.

Description

Technical Field

[0001] This invention relates to the field of medical devices, and more particularly to a liquid chromatography analysis device and the detection components of the liquid chromatography analysis device. Background Technology

[0002] A liquid chromatography analysis device provided by related technology includes a detection assembly for optically detecting a test liquid flowing out of a chromatographic column. The detection assembly includes a light source, a light shaping component, a flowmeter, and a light receiving component arranged sequentially. The flowmeter forms a detection channel through which the test liquid flowing out of the chromatographic column passes. The light shaping component shapes the light emitted by the light source and illuminates the test liquid in the detection channel. The light receiving component receives the optical detection information formed by the light illuminating the test liquid through the detection channel. Since the detection channel is a long and narrow cavity, high precision is required in the optical path from the light source to the flowmeter and in the installation position of the flowmeter to ensure that the light emitted by the light source, after being shaped by the light shaping component, illuminates the test liquid in the detection channel and is received by the light receiving component.

[0003] In related technologies, the optical path from the light source to the flowmeter and the flowmeter itself adopt a separate structure. That is, the light source, light shaping component, and flowmeter are independently set up and installed. To ensure the accuracy of their relative positions, the relative positions of each component need to be adjusted, thus requiring a lot of debugging tooling and time. The position adjustment process during installation is cumbersome, resulting in low production efficiency and high production costs for the detection component in practical applications. Furthermore, in related technologies, LED lights are used as the light source, which presents the following two problems in practical applications: 1) LED lights have a large optical extension, requiring the light shaping component to design a multi-level light-shielding aperture structure to achieve the light spot shaping requirements, which is detrimental to cost control; 2) The technical solutions provided by related technologies are sensitive to the positional tolerance of the light source, and the packaging tolerance of LED lights is relatively large. Therefore, it is difficult to accurately position the light source in this solution, necessitating a separate structure for the light source, light shaping component, and flowmeter to meet debugging requirements. Summary of the Invention

[0004] The first objective of this invention is to provide a liquid chromatography analysis device that aims to solve the technical problem of cumbersome installation and debugging of related technical detection components.

[0005] To achieve the above objectives, the present invention provides a liquid chromatography analysis device, comprising a test solution delivery assembly, a test solution preparation channel, a liquid phase fluid delivery assembly, a chromatographic column, a detection assembly, and a controller;

[0006] The test solution delivery assembly is used to deliver a test solution prepared from at least the sample to the test solution preparation channel;

[0007] The liquid phase fluid delivery assembly is used to drive the test solution in the test solution preparation channel through the chromatographic column via liquid phase fluid, so that the test solution is adsorbed onto the chromatographic column; and to drive the liquid phase fluid through the chromatographic column so that the liquid phase fluid elutes the test solution adsorbed onto the chromatographic column, thereby forming a test solution;

[0008] The detection component is used to perform optical detection on the test liquid flowing out of the chromatographic column and obtain optical detection information;

[0009] The controller is configured to process the light detection information and output the detection result of the sample;

[0010] The detection component includes a light source, a light shaping component, a flow meter, a light receiving component, and a first mounting component.

[0011] The flow device has a detection channel, an inlet, and an outlet. The inlet and the outlet are respectively connected to the detection channel. The inlet is connected to the chromatographic column to allow the test solution flowing out of the chromatographic column to flow into the detection channel. The outlet is used to allow the test solution in the detection channel to flow out.

[0012] The light source is used to emit light;

[0013] The light shaping component is disposed between the light source and the flow meter to shape the light emitted by the light source and then irradiate the test liquid in the detection channel.

[0014] The light receiving component is used to receive the light detection information formed by the test liquid that has been irradiated by the light shaping component and passed through the test channel;

[0015] The first mounting component has a mounting channel for mounting at least the optical shaping component and the flow meter. The mounting channel has an arc-shaped mounting portion for mounting the optical shaping component and / or the flow meter. The central axis of the mounting portion, the central axis of the detection channel, and the optical axis of the optical shaping component are substantially coincident.

[0016] In one embodiment, the central axis of the flow meter is substantially coincident with the central axis of the mounting portion, the central axis of the detection channel, and the optical axis of the optical shaping component.

[0017] In one embodiment, the mounting part is a full circle structure with an arc angle of 360°; or, the mounting part is an arc-shaped structure with an arc angle of less than 360°.

[0018] In one embodiment, the mounting portion is an integral structure that extends continuously in an arc shape around the circumference of the mounting channel;

[0019] Alternatively, the mounting portion may include at least two separate structures arranged in an arc around the mounting channel, with adjacent separate structures abutting each other or spaced apart.

[0020] In one embodiment, the mounting channel includes at least two mounting portions distributed axially along the first mounting component, one of the mounting portions being used to mount the flow meter, and at least the other mounting portion being used to mount at least a portion of the light shaping component;

[0021] And / or, the liquid chromatography analysis device further includes a support plate, and the light shaping component and the flow meter are mounted on the support plate via the first mounting component.

[0022] In one embodiment, the first mounting component is a single, integrally molded component.

[0023] In one embodiment, the first mounting component is an integrally formed cylindrical component, and the mounting portion is a circular through hole formed inside the cylindrical component;

[0024] Alternatively, the first mounting component is an integrally formed open slot-shaped component, and the mounting portion is an arc-shaped groove formed on one side of the open slot-shaped component;

[0025] Alternatively, the first mounting component is an integrally formed component that is at least partially cylindrical and at least partially open slot-shaped. The mounting channel includes at least two mounting portions distributed along the axial direction of the first mounting component, wherein at least one mounting portion is a circular through hole formed in the cylindrical portion, and at least the other mounting portion is an arc-shaped groove formed on one side of the open slot-shaped portion.

[0026] In one embodiment, when the first mounting component is an integrally formed cylindrical component, the mounting channel is a stepped through hole arranged along the axial direction of the first mounting component, and the stepped through hole forms at least two circular through holes with different inner diameters.

[0027] Alternatively, when the first mounting component is an integrally formed open slot-shaped component, the mounting channel is a stepped groove arranged along the axial direction of the first mounting component, and the stepped groove forms at least two arc-shaped grooves with different inner diameters.

[0028] Alternatively, when the first mounting component is a one-piece molded component that is at least partially cylindrical and at least partially open slotted, the mounting channel includes at least one of the circular through holes and at least one arc-shaped groove with an inner diameter different from the inner diameter of the at least one of the circular through holes.

[0029] In one embodiment, the first mounting component includes at least two sub-mounting members, each of the sub-mounting members being at least partially nested and connected to at least another sub-mounting member;

[0030] Each of the sub-mounts has at least one mounting portion, the flower is mounted on the mounting portion of one of the sub-mounts, and at least a portion of the light-shaping component is mounted on the mounting portion of at least another sub-mount.

[0031] In one embodiment, the light shaping component includes a collimating lens disposed between the light source and the flow meter. The collimating lens is used to convert the light emitted by the light source into parallel light and then illuminate the test liquid in the detection channel.

[0032] The mounting channel has a first mounting portion and a second mounting portion distributed along the axial direction of the first mounting component. The central axis of the first mounting portion and the central axis of the second mounting portion are substantially coincident. The inner diameter of the first mounting portion is larger than the inner diameter of the second mounting portion. The flow meter is mounted on the first mounting portion, and the collimating lens is mounted on the second mounting portion.

[0033] In one embodiment, the optical shaping component further includes an aperture stop, which is installed in the mounting channel and positioned between the collimating lens and the flow meter. The aperture stop is used to constrain the light beam that passes through the collimating lens and illuminates the detection channel.

[0034] In one embodiment, the mounting channel further comprises a third mounting portion and a fourth mounting portion. The second mounting portion, the third mounting portion, the fourth mounting portion and the first mounting portion are distributed sequentially along the axial direction of the first mounting component, and the inner diameters of the second mounting portion, the third mounting portion, the fourth mounting portion and the first mounting portion increase sequentially. The aperture stop is mounted on the third mounting portion and the fourth mounting portion.

[0035] In one embodiment, the collimating lens is embedded in the aperture stop at one end facing the flowmeter;

[0036] In one embodiment, the optical component further includes an axial positioning element, which is connected to one end of the flow element and the first mounting component to perform axial positioning of the flow element. The other end of the flow element abuts against one end of the aperture stop to perform axial positioning of the aperture stop, and the other end of the aperture stop abuts against one end of the collimating lens to perform axial positioning of the collimating lens.

[0037] In one embodiment, the light shaping component further includes a first condensing lens and a second condensing lens. The flow device has a first cavity on the side facing the aperture stop, and a second cavity on the side facing away from the aperture stop. The first condensing lens is disposed in the first cavity, and the second condensing lens is disposed in the second cavity.

[0038] In one embodiment, the detection assembly further includes a light guide extending from the light source to the first mounting component, the light guide being used to guide the light emitted by the light source through it to illuminate the light shaping component;

[0039] The light guide component is composed of at least one of optical fiber, light guide rod, light guide tube, and light guide plate;

[0040] The optical axis of the light-emitting end of the light guide is substantially coincident with the central axis of the mounting channel, the central axis of the detection channel, and the optical axis of the light-shaping component.

[0041] In one embodiment, a fifth mounting portion is formed at one end of the mounting channel, and the light-emitting end of the light guide is mounted on the fifth mounting portion;

[0042] Preferably, the optical shaping component includes a collimating lens and an aperture stop. The mounting channel further forms a first mounting portion, a second mounting portion, a third mounting portion, and a fourth mounting portion. The flow meter is mounted on the first mounting portion, the collimating lens is mounted on the second mounting portion, and the aperture stop is mounted on the third and fourth mounting portions. The fifth, second, third, and fourth mounting portions and the first mounting portion are distributed sequentially along the axial direction of the first mounting component.

[0043] In one embodiment, the outer surface of the first mounting component is formed with a first clearance groove extending to the inlet of the flow device and a second clearance groove extending to the outlet of the flow device;

[0044] The liquid chromatography analysis device further includes a first inlet pipe and a first outlet pipe. The first inlet pipe is connected to the inlet through the first clearance groove, and the first outlet pipe is connected to the outlet through the second clearance groove.

[0045] In one embodiment, the first clearance groove and the second clearance groove extend to the end face of the flow device away from the light source.

[0046] In one embodiment, the detection component further includes a second mounting component, on which the light receiving component is mounted, and the first mounting component and the second mounting component are at least partially nested and connected to each other;

[0047] Alternatively, the mounting channel may also be used to mount the optical receiving component.

[0048] In one implementation, the light source is used to emit light of at least a first wavelength and a second wavelength;

[0049] The light receiving component includes a first photodetector, a second photodetector, and a beam splitter. The photodetector, the beam splitter, and the channel are sequentially distributed along the optical axis of the light shaping component. The second photodetector is located to the side of the optical axis of the light shaping component. The beam splitter is used to split the light emitted through the channel into light of the first wavelength and light of the second wavelength, and to allow the light of the first wavelength to be transmitted to the first photodetector and to allow the light of the second wavelength to be reflected to the second photodetector.

[0050] The detection component further includes a second mounting component, on which the first photodetector, the second photodetector, and the beam splitter are all mounted, and the first mounting component and the second mounting component are at least partially nested and connected to each other.

[0051] In one embodiment, the light source includes a single lamp body, which is at least used to emit light of the first wavelength and light of the second wavelength;

[0052] Alternatively, the light source may include at least two lamp bodies, wherein at least one lamp body is used to emit light of the first wavelength and at least another lamp body is used to emit light of the second wavelength.

[0053] A second objective of this invention is to provide a liquid chromatography analysis device, which includes a test solution delivery assembly, a test solution preparation channel, a liquid phase fluid delivery assembly, a chromatographic column, a detection assembly, and a controller.

[0054] The test solution delivery assembly is used to deliver a test solution prepared from at least the sample to the test solution preparation channel;

[0055] The liquid phase fluid delivery assembly is used to drive the test solution in the test solution preparation channel through the chromatographic column via liquid phase fluid, so that the test solution is adsorbed onto the chromatographic column; and to drive the liquid phase fluid through the chromatographic column so that the liquid phase fluid elutes the test solution adsorbed onto the chromatographic column, thereby forming a test solution;

[0056] The detection component is used to perform optical detection on the test liquid flowing out of the chromatographic column and obtain optical detection information;

[0057] The controller is configured to process the light detection information and output the detection result of the sample;

[0058] The detection component includes a light source, a light shaping component, a light receiving component, and an installation structure.

[0059] The detection component has a detection channel, an inlet, and an outlet. The detection channel is located within the mounting structure. The inlet and outlet are respectively connected to the detection channel. The inlet is connected to the chromatographic column to allow the test solution flowing out of the chromatographic column to flow into the detection channel. The outlet is used to allow the test solution in the detection channel to flow out.

[0060] The light source is used to emit light;

[0061] The light shaping component is disposed between the light source and the detection channel to shape the light emitted by the light source and then irradiate the test liquid in the detection channel.

[0062] The light receiving component is used to receive the light detection information formed by the test liquid that has been irradiated by the light shaping component and passed through the test channel;

[0063] The mounting structure has an mounting channel, and the mounting channel has an arc-shaped mounting portion. The mounting portion is used to mount the optical shaping component. The central axis of the mounting portion, the central axis of the detection channel, and the optical axis of the optical shaping component are substantially coincident.

[0064] In one embodiment, the detection assembly further includes a flow meter, which has the detection channel, the inlet, and the outlet, and the flow meter and the mounting structure are integrally formed, with the photoshaping component installed inside the flow meter;

[0065] Alternatively, the detection assembly may further include a flow meter having the detection channel, the inlet, and the outlet, and the flow meter and the mounting structure are separately configured, with at least a portion of the photoshaping component installed within the mounting structure, and the mounting structure installed within the flow meter;

[0066] Alternatively, the detection assembly may further include a flow meter having the detection channel, the inlet, and the outlet, and the flow meter and the mounting structure may be separately configured, with the flow meter and at least a portion of the photoshaping component mounted within the mounting structure.

[0067] In one embodiment, the mounting structure is a single, integrally molded component;

[0068] Optionally, the mounting structure is an integrally formed cylindrical component, and the mounting portion is a circular through hole formed inside the cylindrical component;

[0069] Alternatively, the mounting structure is an integrally formed open slot-shaped component, and the mounting part is an arc-shaped groove formed on one side of the open slot-shaped component;

[0070] Alternatively, the mounting structure is a component integrally formed and at least partially cylindrical and at least partially open slot-shaped, the mounting channel including at least two mounting portions distributed along the axial direction of the mounting structure, wherein at least one mounting portion is a circular through hole formed in the cylindrical portion, and at least another mounting portion is an arc-shaped groove formed on one side of the open slot-shaped portion.

[0071] A third objective of this invention is to provide a liquid chromatography analysis device, which includes a test solution delivery assembly, a test solution preparation channel, a liquid phase fluid delivery assembly, a chromatographic column, a detection assembly, and a controller.

[0072] The test solution delivery assembly is used to deliver a test solution prepared from at least the sample to the test solution preparation channel;

[0073] The liquid phase fluid delivery assembly is used to drive the test solution in the test solution preparation channel through the chromatographic column via liquid phase fluid, so that the test solution is adsorbed onto the chromatographic column; and to drive the liquid phase fluid through the chromatographic column so that the liquid phase fluid elutes the test solution adsorbed onto the chromatographic column, thereby forming a test solution;

[0074] The detection component is used to perform optical detection on the test liquid flowing out of the chromatographic column and obtain optical detection information;

[0075] The controller is configured to process the light detection information and output the detection result of the sample;

[0076] The detection component includes a light source, a light shaping component, a flow meter, a light receiving component, and a first mounting component.

[0077] The flow device has a detection channel, an inlet, and an outlet. The inlet and the outlet are respectively connected to the detection channel. The inlet is connected to the chromatographic column to allow the test solution flowing out of the chromatographic column to flow into the detection channel. The outlet is used to allow the test solution in the detection channel to flow out.

[0078] The light source is used to emit light;

[0079] The light shaping component is disposed between the light source and the flow meter to shape the light emitted by the light source and then irradiate the test liquid in the detection channel.

[0080] The light receiving component is used to receive the light detection information formed by the test liquid that has been irradiated by the light shaping component and passed through the test channel;

[0081] The first mounting component has a mounting channel for mounting at least the optical shaping component and the flow meter, and the mounting channel has an arc-shaped mounting portion for mounting the optical shaping component and / or for mounting the flow meter;

[0082] The light emitted by the light source can be shaped by the light shaping component and then irradiated onto the test liquid in the detection channel. The light receiving component can receive the light detection information formed by the light emitted by the light source, shaped by the light shaping component, and transmitted through the test liquid in the detection channel.

[0083] A fourth objective of the present invention is to provide a detection component comprising a light source, a light shaping component, a flow meter, a light receiving component, and a first mounting component;

[0084] The flow device has a detection channel, an inlet, and an outlet. The inlet and the outlet are respectively connected to the detection channel. The inlet is connected to the chromatographic column to allow the test solution flowing out of the chromatographic column to flow into the detection channel. The outlet is used to allow the test solution in the detection channel to flow out.

[0085] The light source is used to emit light;

[0086] The light shaping component is disposed between the light source and the flow meter to shape the light emitted by the light source and then irradiate the test liquid in the detection channel.

[0087] The light receiving component is used to receive the light detection information formed by the test liquid that has been irradiated by the light shaping component and passed through the test channel;

[0088] The first mounting component has a mounting channel for mounting at least the optical shaping component and the flow meter. The mounting channel has an arc-shaped mounting portion for mounting the optical shaping component and / or the flow meter. The central axis of the mounting portion, the central axis of the detection channel, and the optical axis of the optical shaping component are substantially coincident.

[0089] The liquid chromatography analysis equipment and detection component provided by this invention, by setting a first mounting component with an installation channel in the detection component, and using the installation channel to install an optical shaping component and a flow meter, allows for modular installation of the optical shaping component, flow meter, and first mounting component in the liquid chromatography analysis equipment. This eliminates the need for repeated adjustments to the installation positions of the optical shaping component and flow meter within the liquid chromatography analysis equipment. Furthermore, by providing an arc-shaped mounting portion in the mounting channel for mounting optical shaping components and / or for mounting flow meters, the present invention ensures that the central axis of the mounting portion, the central axis of the detection channel, and the optical axis of the optical shaping component are substantially coincident. This facilitates rapid alignment of the optical shaping component and / or flow meter on the first mounting component, thereby ensuring the installation accuracy of the optical shaping component and flow meter and avoiding the undesirable situation of repeatedly adjusting the position of the optical shaping component and flow meter during the installation process. This improves the installation and debugging efficiency of the optical shaping component and flow meter, and ultimately improves the production efficiency and reduces the production cost of the detection assembly. Attached Figure Description

[0090] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0091] Figure 1 This is a schematic diagram of the liquid circuit of the liquid chromatography analysis device provided in the embodiment of the present invention;

[0092] Figure 2 This is a three-dimensional assembly cross-sectional view of the detection component and the support plate provided in an embodiment of the present invention;

[0093] Figure 3 This is a schematic cross-sectional view of the assembly of the detection component and the support plate provided in an embodiment of the present invention from one perspective;

[0094] Figure 4This is a schematic cross-sectional view of the assembly of the detection component and the support plate provided in an embodiment of the present invention from another perspective;

[0095] Figure 5 This is a perspective view of the first mounting component provided in an embodiment of the present invention;

[0096] Figure 6 This is a cross-sectional schematic diagram of the first mounting component provided in an embodiment of the present invention.

[0097] Reference numerals in the attached figures: 10. Liquid chromatography analysis equipment; 100. Detection component; 110. Light source; 120. Optical shaping component; 121. Collimating lens; 122. Aperture stop; 123. First condenser lens; 124. Second condenser lens; 130. Flowmeter; 131. Detection channel; 132. Liquid inlet; 133. Liquid outlet; 140. Light receiving component; 141. First photodetector; 142. Second photodetector; 143. Spectrometer; 144. First filter; 145. Second filter; 150. First mounting component; 151. Mounting channel; 1511. First mounting section; 1512. Second mounting section; 1 513. Third mounting section; 1514. Fourth mounting section; 1515. Fifth mounting section; 152. First clearance groove; 153. Second clearance groove; 160. Light guide; 170. Axial positioning component; 180. Second pressure ring; 190. Third pressure ring; 101. Second mounting component; 200. Liquid phase fluid delivery assembly; 210. First drive pump; 220. Second drive pump; 300. Chromatographic column; 400. Sample delivery assembly; 410. Sampling component; 420. Reaction vessel; 430. Sample delivery path; 500. Sample preparation channel; 600. Reversing valve; 700. Support plate; 20. First container; 30. Second container. Detailed Implementation

[0098] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0099] The embodiments of the present invention are applicable to scenarios where optical devices are required to detect the test solution separated by elution from a chromatographic column.

[0100] like Figures 1 to 6As shown, the liquid chromatography analysis apparatus 10 provided in the first aspect of the present invention includes a liquid phase fluid delivery assembly 200, a chromatographic column 300, a detection assembly 100, and a controller. The chromatographic column 300 is used to adsorb a test solution prepared from at least a sample. The liquid phase fluid delivery assembly 200 is used to drive a liquid phase fluid through the chromatographic column 300, so that the liquid phase fluid elutes the test solution adsorbed on the chromatographic column 300, thereby forming a test solution. The detection assembly 100 is used to perform optical detection on the test solution eluting from the chromatographic column 300 and obtain optical detection information. The controller is configured to process the optical detection information and output the detection result of the sample. The liquid chromatography analysis apparatus 10 is used to perform chromatographic analysis on the sample. The sample can be a sample collected from a human or animal, or other samples. The liquid phase fluid, also known as the eluent or elution phase, can be used to elute the test solution adsorbed on the chromatographic column 300, so that the detection assembly 100 can detect different components eluted from the chromatographic column 300.

[0101] In one embodiment, the liquid chromatography analysis apparatus 10 further includes a reagent delivery assembly 400 and a reagent preparation channel 500. The reagent delivery assembly 400 is used to deliver a reagent prepared from at least the sample to the reagent preparation channel 500. The liquid flow delivery assembly 200 is used to drive the reagent in the reagent preparation channel 500 through the chromatographic column 300 via liquid flow, so that the reagent is adsorbed onto the chromatographic column 300; and to drive the liquid flow through the chromatographic column 300 so that the liquid flow elutes the reagent adsorbed on the chromatographic column 300, thereby forming the analyte. The reagent preparation channel 500 provides a transfer point for the reagent. After the reagent preparation is completed, it is first delivered to the reagent preparation channel 500 by the reagent delivery assembly 400, and then the liquid flow delivery assembly 200 drives the reagent in the reagent preparation channel 500 through the chromatographic column 300 via liquid flow. A certain pressure is required to drive the reagent from the reagent preparation channel 500 to the chromatographic column 300. In this embodiment, the liquid phase fluid delivery component 200 is reused to drive the flow of the test solution to the chromatographic column 300. That is, the liquid phase fluid delivery component 200 is used to drive the liquid phase fluid to the chromatographic column 300 and to drive the test solution to the chromatographic column 300. In this way, it is not necessary to set up other driving components to drive the test solution to the chromatographic column 300, and it is also beneficial to reduce the rated working pressure requirement of the test solution delivery component 400.

[0102] In one implementation, the detection assembly 100 includes a light source 110, a light shaping component 120, a flow channel 130, and a light receiving component 140. The flow channel 130 is used for the flow of the analyte eluted from the chromatographic column 300. The light source 110 emits light. The light shaping component 120 shapes the light emitted by the light source 110 and irradiates the analyte in the flow channel 130. The light receiving component 140 receives the light detection information formed by the light irradiated by the light shaping component 120 and transmitted through the analyte in the flow channel 130. The flow channel 130 provides a detection site for the analyte. The light source 110 is the light signal emitting end of the detection assembly 100, used to emit detection light to the analyte. The light shaping component 120 adjusts the light emitted by the light source 110 so that the light emitted by the light source 110 is better focused on the analyte. The light receiving component 140 is the light signal receiving end of the detection assembly 100, used to detect the light signal generated by the detection light irradiating the analyte.

[0103] In one implementation, the flow meter 130 has a detection channel 131, an inlet 132, and an outlet 133. The inlet 132 and outlet 133 are respectively connected to the detection channel 131. The inlet 132 is connected to the chromatographic column 300 to allow the test solution flowing out of the chromatographic column 300 to flow into the detection channel 131, and the outlet 133 is used to allow the test solution in the detection channel 131 to flow out. A light shaping component 120 is disposed between the light source 110 and the flow meter 130 to shape the light emitted by the light source 110 and then irradiate the test solution in the detection channel 131. A light receiving component 140 is used to receive the light detection information formed by the light irradiated by the light shaping component 120 and transmitted through the test solution in the detection channel 131. In practical applications, the test solution eluted from the chromatographic column 300 flows into the detection channel 131 from the inlet 132, flows through the detection channel 131 to the outlet 133, and flows out of the flow meter 130 from the outlet 133.

[0104] In one implementation, the central axis of the detection channel 131 and the optical axis of the light shaping component 120 are substantially coincident. This ensures that the light shaped by the light shaping component 120 can smoothly illuminate the test liquid passing through the detection channel 131 and be received by the light receiving component 140. The substantial coincidence of the central axis of the detection channel 131 and the optical axis of the light shaping component 120 includes: the central axis of the detection channel 131 and the optical axis of the light shaping component 120 being collinear; or the central axis of the detection channel 131 and the optical axis of the light shaping component 120 not being collinear but having a very small offset distance (e.g., an offset distance between ±0.1 mm); or forming a very small tilt angle between them (e.g., a tilt angle greater than 0° and less than or equal to 1°).

[0105] In one implementation, the detection assembly 100 further includes a first mounting component 150, which is used to mount at least the optical shaping component 120 and the flow meter 130. In this embodiment, the optical shaping component 120 and the flow meter 130 are integrated and mounted on the first mounting component 150. Thus, in actual production, the optical shaping component 120 and the flow meter 130 can be first mounted on the first mounting component 150 to assemble the optical shaping component 120, the flow meter 130, and the first mounting component 150 into a module. Then, this module is installed as a whole on the liquid chromatography analysis device 10. This allows for the overall modular installation of the optical shaping component 120, the flow meter 130, and the first mounting component 150 on the liquid chromatography analysis device 10, without the need for repeated adjustments to the mounting positions of the optical shaping component 120 and the flow meter 130 on the liquid chromatography analysis device 10.

[0106] In one embodiment, the first mounting member 150 has a mounting channel 151 for mounting at least the optical shaping member 120 and the flow meter 130. The mounting channel 151 also has an arc-shaped mounting portion for mounting the optical shaping member 120 and / or the flow meter 130. The central axis of the mounting portion, the central axis of the detection channel 131, and the optical axis of the optical shaping member 120 substantially coincide. The central axis of the mounting channel 151 is coaxial with the central axis of the mounting portion. By using an arc-shaped mounting part to install the optical shaping component 120 and / or the flow meter 130, it is only necessary to set the inner diameter of the mounting part to match the outer diameter of the optical shaping component 120 and / or the flow meter 130 to be installed. This allows for quick alignment of the optical shaping component 120 and / or the flow meter 130 on the first mounting part 150 without the need for repeated adjustments to the position of the optical shaping component 120 and the flow meter 130. This not only meets the installation position accuracy requirements of the optical shaping component 120 and the flow meter 130, but also enables the optical shaping component 120 and the flow meter 130 to be adjusted without adjustment, effectively improving the installation and adjustment efficiency of the optical shaping component 120 and the flow meter 130. Ultimately, this helps to improve the production efficiency of the detection component 100 and reduce the production cost of the detection component 100. In this embodiment, the first mounting component 150, the flow meter 130, and the optical shaping component 120 are independently configured components. This ensures that the configuration of the mounting channel 151 does not affect the structure of the flow meter 130 and the optical shaping component 120. Of course, in alternative embodiments, the first mounting component 150 can be integrated with either the flow meter 130 or the optical shaping component 120. Specifically, the mounting channel 151 can be provided in the flow meter 130 for mounting the optical shaping component 120, or vice versa.

[0107] In one implementation, the outer surface of the optical shaping component 120 and / or the flow meter 130 abuts against the arc-shaped mounting portion. In this way, by simply setting the inner diameter of the mounting portion to match the outer diameter of the optical shaping component 120 and / or the flow meter 130 to be installed, the optical shaping component 120 and / or the flow meter 130 can be quickly aligned on the first mounting component 150, which facilitates the elimination of adjustment for the optical shaping component 120 and the flow meter 130.

[0108] In one implementation, the mounting portion is a fully circular structure with an arc angle of 360°, that is, the mounting portion is a circumferentially closed circular hole, and the light shaping component 120 and / or the flower 130 passes through the circular hole of the first mounting component 150. The light shaping component 120 and / or the flower 130 are assembled with the first mounting component 150 in a nested assembly manner, that is, one is inserted into the other, or one is fitted over the other. Of course, in specific applications, the mounting portion is not limited to this arrangement. For example, as an alternative implementation, the mounting portion is an arc-shaped structure with an arc angle of less than 360°, that is, the mounting portion is a non-fully circular structure that is not circumferentially closed and has at least a partial notch in the circumferential direction. Specifically, the non-fully circular structure is a groove with a notch.

[0109] In one implementation, the central axis of the flow meter 130 is substantially coincident with the central axis of the mounting section, the central axis of the detection channel 131, and the optical axis of the optical shaping component 120. The inner surface of the flow meter 130 (i.e., the inner wall of the detection channel 131) is arc-shaped, and the outer surface of the flow meter 130 is also arc-shaped, which facilitates the installation of the flow meter 130 and ensures the accuracy of the installation position of the flow meter 130.

[0110] In one embodiment, the mounting part is an integral structure that extends continuously in an arc shape around the mounting channel 151 in the circumferential direction. In this embodiment, the mounting part is either a single-piece circular structure or a single-piece non-circular arc-shaped structure; that is, the mounting part is a single-piece component with a circular or non-circular arc shape, rather than being formed by splicing or combining multiple components in a partial circumferential direction. This design facilitates the manufacturing of the mounting part and helps ensure the accuracy of its arc shape. Of course, in specific applications, the mounting part is not limited to this arrangement. For example, as an alternative implementation, the mounting part includes at least two separate structures arranged in an arc around the mounting channel 151, with adjacent separate structures abutting each other. In this alternative implementation, the mounting part is a complete circular structure or a non-complete circular arc structure formed by splicing at least two separate structures around the circumference. Alternatively, as another alternative implementation, the mounting part includes at least two separate structures arranged in an arc around the circumference of the mounting channel 151, with adjacent separate structures spaced apart. In this alternative implementation, the mounting part is a structure formed by at least two separate structures arranged in an arc around the circumference.

[0111] In one embodiment, the mounting portion includes a fully circular arc-shaped surface or a non-fully circular arc-shaped surface; or, the mounting portion may also include fully circular arc-shaped ribs or non-fully circular arc-shaped ribs; or, the mounting portion may also include protrusions distributed in an arc shape.

[0112] In one embodiment, the mounting channel 151 includes at least two mounting portions distributed axially along the first mounting member 150. One mounting portion is used to mount the flower 130, and at least the other mounting portion is used to mount at least a portion of the optical shaping member 120. In this embodiment, at least two mounting portions are formed for mounting the flower 130 and at least a portion of the optical shaping member 120, respectively, meaning that the flower 130 and at least a portion of the optical shaping member 120 form a nested assembly structure with the first mounting member 150. The optical shaping member 120 may all be nested with the mounting portions, or a portion may be nested with the mounting portion and another portion may be nested with the flower 130. Of course, in specific applications, the mounting channel 151 is not limited to this configuration. For example, as an alternative implementation, the mounting channel 151 forms a single mounting portion, the flow meter 130 is embedded in the mounting channel 151, and the optical shaping component 120 is embedded in the flow meter 130; or, as another alternative implementation, the mounting channel 151 forms a single mounting portion, the optical shaping component 120 is embedded in the mounting channel 151, and the flow meter 130 is embedded in the optical shaping component 120.

[0113] In one embodiment, the first mounting component 150 is a single, integrally formed component. In this embodiment, the first mounting component 150 is a single component, rather than being assembled from two or more components. This helps to ensure the positional accuracy of each part on the first mounting component 150.

[0114] In one embodiment where the first mounting component 150 is integrally formed, the first mounting component 150 is an integrally formed cylindrical component, and the mounting portion is a circular through hole formed inside the cylindrical component. That is, the first mounting component 150 is a component with a cylindrical outer surface and a hollow through hole inside. The flow device 130 and the smoothing component 120 are at least partially inserted and housed in the hollow through hole of the first mounting component 150. The outer surfaces of the flow device 130 and the smoothing component 120 are in contact with the inner surface of the circular through hole, respectively. In this way, on the one hand, it is convenient to achieve the centering effect after the flow device 130 and the smoothing component 120 are installed; on the other hand, the first mounting component 150 can be used to protect the flow device 130 and the smoothing component 120.

[0115] In a further embodiment of the first implementation where the first mounting component 150 is an integrally formed component, the first mounting component 150 is an integrally formed cylindrical component, and the mounting channel 151 is a stepped through hole arranged axially along the first mounting component 150. The stepped through hole forms at least two circular through holes with different inner diameters. Specifically, the stepped through hole consists of two adjacent through holes with different diameters to form a stepped structure. The flow device 130 and at least a portion of the smoothing component 120 are respectively installed through the circular through holes of different inner diameters in the stepped through hole. In this embodiment, the hollow through hole of the first mounting component 150 is set as a stepped through hole formed by multiple circular through holes. On the one hand, the circular through holes of different diameters can be used to abut and cooperate with different components. On the other hand, the steps formed by the stepped through hole can be used to axially limit the flow device 130 and / or smoothing component 120 components installed in the mounting channel 151.

[0116] In a second embodiment where the first mounting component 150 is integrally formed, the first mounting component 150 is an integrally formed open slot-shaped component, and the mounting portion is an arc-shaped groove formed on one side of the open slot-shaped component. The open slot-shaped component is equivalent to a component with a notch formed after a portion of the circumferential part is cut off from a cylindrical component. In this embodiment, the first mounting component 150 is a component with an arc-shaped groove formed on one side. This arc-shaped groove can be a semi-circular arc-shaped groove; or it can be an arc-shaped groove smaller than a semi-circle (e.g., a quarter-circle arc-shaped groove or other arc-shaped grooves smaller than a semi-circle); or it can be an arc-shaped groove larger than a semi-circle (e.g., a two-thirds-circle arc-shaped groove or other arc-shaped grooves larger than a semi-circle). The outer surfaces of the flow device 130 and the smoothing component 120 respectively contact the arc-shaped surface of the arc-shaped groove.

[0117] In a further embodiment of the second implementation where the first mounting component 150 is an integrally formed component, the first mounting component 150 is an integrally formed open slot-shaped component, and the mounting channel 151 is a stepped groove arranged axially along the first mounting component 150. The stepped groove forms at least two arc-shaped grooves with different inner diameters. Specifically, the stepped groove consists of two adjacent arc-shaped grooves with different inner diameters to form a stepped structure. The flow device 130 and at least a portion of the smoothing component 120 are respectively installed in the arc-shaped grooves with different inner diameters in the stepped groove. In this embodiment, the through slot of the first mounting component 150 is set as a stepped groove formed by multiple arc-shaped grooves. On the one hand, the arc-shaped grooves with different inner diameters can be used to abut and cooperate with different components. On the other hand, the steps formed by the stepped grooves can be used to axially limit the flow device 130 and / or smoothing component 120 components installed in the mounting channel 151.

[0118] In a third embodiment where the first mounting component 150 is integrally formed, the first mounting component 150 is integrally formed and at least partially cylindrical and at least another partially open slot-shaped. The mounting channel 151 includes at least two mounting portions distributed along the axial direction of the first mounting component 150, wherein at least one mounting portion is a circular through hole formed within the cylindrical portion, and at least the other mounting portion is an arc-shaped groove formed on one side of the open slot-shaped portion. The first mounting component 150 includes at least one cylindrical first portion and at least one open slot-shaped second portion, and the at least one first portion and at least one second portion are distributed along the axial direction of the first mounting component 150. For example, one end of the first part can be connected to one end of the second part; or, one end of the first part can be connected to one end of a second part, and the other end of the first part can be connected to one end of another second part; or, one end of the second part can be connected to one end of a first part, and the other end of the second part can be connected to one end of another first part; or, one end of a first part can be connected to one end of a second part, and the other end of the first part can be connected to one end of another first part; or, one end of a second part can be connected to one end of a first part, and the other end of the second part can be connected to one end of another second part.

[0119] In a further embodiment of the third implementation where the first mounting component 150 is a monolithically formed component, the mounting channel 151 includes at least one circular through-hole and at least one arc-shaped groove with an inner diameter different from that of the at least one circular through-hole. The circular through-hole and the arc-shaped groove also form a stepped structure. The flow device 130 and at least a portion of the smoothing component 120 are respectively installed through the circular through-hole and the arc-shaped groove.

[0120] In the above scheme, the first mounting component 150 is a single integrally formed component. Of course, in specific applications, the first mounting component 150 can also be formed by combining multiple nested components. For example, as an alternative implementation, the first mounting component 150 includes at least two sub-mounting parts, each sub-mounting part being at least partially nested and connected to at least another sub-mounting part; each sub-mounting part has at least one mounting portion, the flow meter 130 is mounted on the mounting portion of one sub-mounting part, and at least a portion of the light shaping component 120 is mounted on the mounting portion of at least another sub-mounting part. The optical shaping component 120 includes multiple optical shaping elements. In specific applications, the multiple optical shaping elements and the flow meter 130 can be individually nested in a sub-mount, and then individually nested in a sub-mount that serves as the main body; or, the multiple optical shaping elements and the flow meter 130 can be individually nested in a sub-mount, and then the sub-mounts can be nested and connected to each other; or, the multiple optical shaping elements and the flow meter 130 can be individually nested in a sub-mount, and then some of the sub-mounts can be further nested and cooperated with each other before being nested in a sub-mount that serves as the main body.

[0121] In one implementation, the sub-mounting component can be a cylindrical part or a part with an open slot.

[0122] In one embodiment, the light shaping component 120 includes a collimating lens 121, which is disposed between the light source 110 and the flow channel 130. The collimating lens 121 is used to convert the light emitted by the light source 110 into parallel light and then illuminate the test liquid in the detection channel 131. The collimating lens 121 is located downstream of the light path of the light source 110 and collimates the light emitted by the light source 110 to obtain parallel light.

[0123] In one embodiment, the mounting channel 151 has a first mounting portion 1511 and a second mounting portion 1512 distributed along the axial direction of the first mounting component 150. The central axis of the first mounting portion 1511 and the central axis of the second mounting portion 1512 are substantially coincident. The inner diameter of the first mounting portion 1511 is different from the inner diameter of the second mounting portion 1512. The flow meter 130 is mounted in the first mounting portion 1511, and the collimating lens 121 is mounted in the second mounting portion 1512. The first mounting portion 1511 can be a circular through hole or an arc-shaped groove, and the second mounting portion 1512 can also be a circular through hole or an arc-shaped groove. In this embodiment, the collimating lens 121 and the flow meter 130 are respectively mounted in different mounting portions in the mounting channel 151. The structure is simple, and for collimating lenses 121 and flow meters 130 of different specifications, only the first mounting component 150 needs to be designed accordingly, without modifying the collimating lens 121 and the flow meter 130. Of course, in specific applications, as an alternative implementation, the mounting channel 151 may only have one of the first mounting portion 1511 and the second mounting portion 1512 without forming the other. For example, the mounting channel 151 may only have the first mounting portion 1511 without forming the second mounting portion 1512, and the collimating lens 121 may be nested and mounted on the flow meter 130, which may be mounted on the first mounting portion 1511.

[0124] In one implementation, the inner diameter of the first mounting portion 1511 is the same as the outer diameter of the flower 130, and the flower 130 is directly mounted on the first mounting portion 1511. Of course, in specific applications, as an alternative implementation, the flower 130 may first be nested within one or two cylindrical components, and then mounted on the first mounting portion 1511 through the one or two cylindrical components.

[0125] In one embodiment, the inner diameter of the second mounting portion 1512 is the same as the outer diameter of the collimating lens 121, and the collimating lens 121 is directly mounted on the second mounting portion 1512. Of course, in specific applications, as an alternative embodiment, the collimating lens 121 may also be nested inside one or two cylindrical components, and then mounted on the second mounting portion 1512 through the one or two cylindrical components.

[0126] In one embodiment, the inner diameter of the first mounting portion 1511 is larger than the inner diameter of the second mounting portion 1512. The outer diameter of the flow meter 130 is larger than the outer diameter of the collimating lens 121.

[0127] In one embodiment, the light shaping component 120 further includes an aperture stop 122, which is installed within the mounting channel 151 and positioned between the collimating lens 121 and the flow channel 130. The aperture stop 122 is used to constrain the light beam that passes through the collimating lens 121 and illuminates the detection channel 131. The aperture stop 122 is mainly used to limit the size of the light beam emitted by the light source 110 and directed towards the detection channel 131, thereby constraining the divergence angle of the light emitted by the light source 110.

[0128] In one embodiment, the mounting channel 151 further comprises a third mounting portion 1513 and a fourth mounting portion 1514. The second mounting portion 1512, the third mounting portion 1513, the fourth mounting portion 1514, and the first mounting portion 1511 are sequentially distributed along the axial direction of the first mounting member 150, and the inner diameters of the second mounting portion 1512, the third mounting portion 1513, the fourth mounting portion 1514, and the first mounting portion 1511 increase sequentially. The aperture stop 122 is mounted on the third mounting portion 1513 and the fourth mounting portion 1514. In this embodiment, the outer surface of the aperture stop 122 is a stepped component, and the mounting channel 151 is correspondingly provided with stepped mounting portions for mounting the aperture stop 122. Of course, in specific applications, as an alternative implementation, the outer surface of the aperture stop 122 can also be set to be cylindrical, and the mounting channel 151 only needs to be provided with one mounting part (the third mounting part 1513 and the fourth mounting part 1514) to install the aperture stop 122; or, as another alternative implementation, the aperture stop 122 can also be nested and installed on the flow meter 130.

[0129] In one implementation, the flow device 130 can be directly mounted on the third mounting portion 1513 and the fourth mounting portion 1514. Of course, in specific applications, as an alternative implementation, the flow device 130 can also be nested within one or two cylindrical components, and then mounted on the third mounting portion 1513 and the fourth mounting portion 1514 through the one or two cylindrical components.

[0130] In one implementation, the end of the collimating lens 121 facing the flow meter 130 is embedded within the aperture stop 122. This allows for improved reliability in the positioning and alignment of the collimating lens 121 and the aperture stop 122 through their cooperation. However, in specific applications, the arrangement of the collimating lens 121 and the aperture stop 122 is not limited to this. As an alternative implementation, the end of the collimating lens 121 may not be embedded within the aperture stop 122, and the end of the collimating lens 121 may engage with the aperture stop 122 through end-face contact; or, as another alternative implementation, the end of the aperture stop 122 facing away from the flow meter 130 may be embedded within the collimating lens 121.

[0131] In one embodiment, the optical assembly further includes an axial positioning member 170. The axial positioning member 170 is connected to one end of the flowper 130 and the first mounting component 150 to axially position the flowper 130. The other end of the flowper 130 abuts against one end of the aperture stop 122 to axially position the aperture stop 122. The other end of the aperture stop 122 abuts against one end of the collimating lens 121 to axially position the collimating lens 121. The axial positioning member 170 can sequentially press the flowper 130, the aperture stop 122, and the collimating lens 121 into the mounting channel 151 to achieve axial positioning of the flowper 130, the aperture stop 122, and the collimating lens 121 within the mounting channel 151.

[0132] In one embodiment, the axial positioning member 170 is a first pressure ring, which is threadedly connected to the first mounting component 150. One end of the first pressure ring presses against the flow meter 130 to sequentially press the flow meter 130, the aperture stop 122, and the collimating lens 121 into the mounting channel 151. Of course, in specific applications, the arrangement of the axial positioning member 170 is not limited to this. For example, as an alternative embodiment, the axial positioning member 170 can also be connected to the first mounting component 150 by screws or clips.

[0133] In one embodiment, the light shaping component 120 further includes a first condensing lens 123. A first cavity is provided on the side of the flower 130 facing the aperture stop 122, and the first condensing lens 123 is disposed within the first cavity. The first condensing lens 123 is used to focus the light emitted through the aperture stop 122 onto the test liquid in the detection channel 131. In this embodiment, by providing a first cavity in the flower 130 to mount the first condensing lens 123, it is beneficial to improve the structural compactness of the detection component 100 and to improve the assembly position accuracy of the first condensing lens 123 and the flower 130. Of course, in specific applications, the mounting method of the first condensing lens 123 is not limited to this. For example, as an alternative embodiment, a mounting part can also be provided in the mounting channel 151 for mounting the first condensing lens 123.

[0134] In one implementation, the detection assembly 100 further includes a second pressure ring 180, which is connected to the flow meter 130 and presses and positions the first condenser lens 123 within the first cavity. Of course, in specific applications, the positioning method of the first condenser lens 123 is not limited to this.

[0135] In one embodiment, the light shaping component 120 further includes a second condenser lens 124. A second cavity is provided on the side of the flow meter 130 opposite to the aperture stop 122, and the second condenser lens 124 is disposed within the second cavity. The second condenser lens 124 is used to converge the light emitted through the flow meter 130 to illuminate the light receiving component 140. The mounting principle and alternative mounting methods of the second condenser lens 124 can be referred to the first condenser lens 123, and will not be detailed here.

[0136] In one implementation, the detection assembly 100 further includes a third pressure ring 190, which is connected to the flow meter 130 and presses and positions the second condenser lens 124 within the second cavity. Of course, in specific applications, the positioning method of the second condenser lens 124 is not limited to this.

[0137] In one embodiment, the liquid chromatography analysis apparatus 10 further includes a support plate 700, on which the optical shaping component 120 and the flow meter 130 are mounted via a first mounting component 150. In prior art, the optical shaping component 120 and the flow meter 130 were separately mounted on the support plate 700, requiring cumbersome adjustments during installation to ensure their accurate positioning. In this embodiment, by first mounting the optical shaping component 120 and the flow meter 130 onto the first mounting component 150 with a mounting channel 151, and then mounting them onto the support plate 700 via the first mounting component 150, the mounting channel 151 allows for rapid alignment and installation of the optical shaping component 120 and the flow meter 130, effectively solving the problem of cumbersome installation and adjustment of the optical shaping component 120 and the flow meter 130.

[0138] In one implementation, the detection assembly 100 further includes a light guide 160, which extends from the light source 110 to the first mounting component 150. The light guide 160 is used to guide the light emitted by the light source 110 to the light shaping component 120. The light guide 160 reduces the optical expansion of the light source 110, thereby simplifying the structural complexity of the light shaping component 120 and reducing the cost of the detection assembly 100. On the other hand, the specific light beam selection characteristics of the light guide 160 can compensate for the poor positioning accuracy of the light source 110, thus facilitating the elimination of adjustment between the light source 110 and the light guide 160.

[0139] In one embodiment, the light guide 160 is composed of at least one of an optical fiber, a light guide rod, a light guide tube, and a light guide plate.

[0140] In one implementation, the light guide 160 is an optical fiber. The optical fiber is a flexible light guide 160, which has good bendability and is convenient for integrating the optical paths of the light source 110 and the light shaping component 120 together. The optical fiber has the advantages of low optical loss, high optical transmission efficiency, strong anti-interference ability and low cost.

[0141] In one implementation, the optical axis of the light-emitting end of the light guide 160 is substantially coincident with the central axis of the mounting channel 151, the central axis of the detection channel 131, and the optical axis of the light-shaping component 120. This reduces the impact of poor positioning accuracy of the light source 110 on the accuracy of the light detection results of the liquid under test.

[0142] In one embodiment, a fifth mounting portion 1515 is formed at one end of the mounting channel 151, and the light-emitting end of the light guide 160 is mounted in the fifth mounting portion 1515. The light guide 160 has high positioning accuracy. By assembling and fixing the light guide 160, the light shaping component 120, and the flow meter 130 together in the mounting channel 151 of the first mounting component 150, the relative positional accuracy requirements between the three can be met, which is beneficial to achieving adjustment-free operation between the light source 110, the light guide 160, the light shaping component 120, and the flow meter 130.

[0143] In one embodiment, the optical shaping component 120 includes a collimating lens 121 and an aperture stop 122. The mounting channel 151 also has a first mounting portion 1511, a second mounting portion 1512, a third mounting portion 1513, and a fourth mounting portion 1514. The flow meter 130 is mounted on the first mounting portion 1511, the collimating lens 121 is mounted on the second mounting portion 1512, and the aperture stop 122 is mounted on the third mounting portion 1513 and the fourth mounting portion 1514. The fifth mounting portion 1515, the second mounting portion 1512, the third mounting portion 1513, the fourth mounting portion 1514, and the first mounting portion 1511 are distributed sequentially along the axial direction of the first mounting component 150. The light-emitting end of the light guide 160 passes through one end of the first mounting component 150 and is installed in the first mounting component 150. The collimating lens 121, the aperture stop 122, and the flow meter 130 pass through the other end of the first mounting component 150 and are installed in the first mounting component 150 in sequence.

[0144] In one embodiment, the outer surface of the first mounting component 150 has a first clearance groove 152 extending to the inlet 132 of the flow meter 130 and a second clearance groove 153 extending to the outlet 133 of the flow meter 130. The liquid chromatography analysis apparatus 10 also includes a first inlet pipe and a first outlet pipe. The first inlet pipe is connected to the inlet 132 via the first clearance groove 152, and the first outlet pipe is connected to the outlet 133 via the second clearance groove 153. The first clearance groove 152 and the second clearance groove 153 are mainly used to ensure that after the flow meter 130 is installed in the mounting channel 151 of the first mounting component 150, it can still be connected to the external liquid circuit through the first inlet pipe and the first outlet pipe.

[0145] In one embodiment, the first clearance groove 152 and the second clearance groove 153 extend to the end face of the flower 130 away from the light source 110. The first clearance groove 152 extends from the end face of the first mounting member 150 away from the light source 110 along the axial direction of the first mounting member 150 at least to the inlet 132, and the second clearance groove 153 extends from the end face of the first mounting member 150 away from the light source 110 along the axial direction of the first mounting member 150 at least to the outlet 133. The inlet 132 is provided with a protruding inlet connector, and the outlet 133 is provided with a protruding outlet connector. In practical applications, the inlet and outlet connectors are first installed on the flow meter 130, and then the flow meter 130 is pushed into the installation channel 151 along the axial direction of the first mounting component 150. The first clearance groove 152 and the second clearance groove 153 extend to the end face of the flow meter 130 away from the light source 110, which helps to prevent interference between the inlet and outlet connectors and the flow meter 130 during the process of pushing the flow meter 130 into the installation channel 151.

[0146] In one embodiment, the detection component 100 further includes a second mounting component 101, on which the light receiving component 140 is mounted. The first mounting component 150 and the second mounting component 101 are at least partially nested and connected to each other. In this embodiment, the light receiving component 140 is first mounted on the second mounting component 101, and then mounted on the first mounting component 150 via the second mounting component 101, thereby improving the relative positional accuracy of the light receiving component 140 with respect to the flow meter 130 and the light shaping component 120. Of course, in specific applications, the mounting method of the light receiving component 140 is not limited to this. For example, as an alternative embodiment, the light receiving component 140 can also be directly mounted in the mounting channel 151, that is, the mounting channel 151 can also be used to mount the light receiving component 140.

[0147] In one implementation, the light source 110 is used to emit light of at least a first wavelength and a second wavelength. The light receiving component 140 includes a first photodetector 141, a second photodetector 142, and a beam splitter 143. The photodetectors, beam splitter 143, and flow channel 130 are sequentially distributed along the optical axis of the light shaping component 120. The second photodetector 142 is located laterally to the optical axis of the light shaping component 120. The beam splitter 143 is used to split the light emitted through the flow channel into light of the first wavelength and light of the second wavelength, allowing the first wavelength light to be transmitted to the first photodetector 141 and the second wavelength light to be reflected to the second photodetector 142. One of the first wavelength light and the second wavelength light is the detection light, and the other is the reference light. This implementation uses dual-wavelength light to detect the test liquid, which helps to ensure the accuracy and reliability of the detection results.

[0148] In one implementation, the detection assembly 100 further includes a second mounting component 101, on which the first photodetector 141, the second photodetector 142, and the beam splitter 143 are all mounted. The first mounting component 150 and the second mounting component 101 are at least partially nested and connected to each other. In this embodiment, the first photodetector 141, the second photodetector 142, and the beam splitter 143 are first mounted on the second mounting component 101, and then the first photodetector 141, the second photodetector 142, the beam splitter 143, and the second mounting component 101 are mounted as an integrated assembly on the first mounting component 150. This ensures the relative positional accuracy of the first photodetector 141, the second photodetector 142, and the beam splitter 143 with the flow meter 130 and the optical shaping component 120, thereby simplifying the overall structure of the optical detection assembly 100 and eliminating the need for adjustment. Of course, in specific applications, as an alternative implementation, the arrangement of the first photodetector 141, the second photodetector 142, and the beam splitter 143 is not limited to this. For example, as an alternative implementation, at least one of the first photodetector 141, the second photodetector 142, and the beam splitter 143 may be mounted on the first mounting component 150.

[0149] In one embodiment, the light receiving component 140 further includes a first filter 144, which is mounted on the second mounting component 101 and located between the beam splitter 143 and the first photodetector 141.

[0150] In one embodiment, the light receiving component 140 further includes a second filter 145, which is mounted on the second mounting component 101 and located between the beam splitter 143 and the second photodetector 142.

[0151] In one implementation, the beam splitter 143 is a dichroic mirror.

[0152] In one implementation, the light source 110 includes a single lamp body, which is used to emit light of at least a first wavelength and light of at least a second wavelength. Alternatively, the light source 110 includes at least two lamp bodies, wherein at least one lamp body is used to emit light of the first wavelength and at least another lamp body is used to emit light of the second wavelength. That is, the first wavelength light and the second wavelength light can be emitted by the same lamp body or by different lamp bodies.

[0153] In one embodiment, the liquid phase fluid delivery assembly 200 includes a first drive pump 210 and a second drive pump 220. The first drive pump 210 is used to draw a first liquid from the first container 20 and deliver the drawn first liquid toward the chromatographic column 300. The second drive pump 220 is used to draw a second liquid from the second container 30 and deliver the drawn second liquid toward the chromatographic column 300.

[0154] In one implementation, the ionic strength of the first liquid is less than that of the second liquid. Specifically, the first liquid may be liquid A, and the second liquid may be liquid B.

[0155] In one embodiment, the first liquid contains a first buffer salt, and the second liquid contains a second buffer salt; the concentration of the first liquid is the concentration of the first buffer salt in the first liquid, and the concentration of the second liquid is the concentration of the second buffer salt in the second liquid. The first buffer salt and the second buffer salt are either buffer salts of the same composition or buffer salts of two different compositions, that is, the buffer salt in the first liquid and the buffer salt in the second liquid can be the same buffer salt or different buffer salts.

[0156] As one implementation method, the above-mentioned sample is a blood sample.

[0157] In one embodiment, the liquid chromatography analysis apparatus 10 further includes a reversing valve 600, with the chromatographic column 300 connected between the reversing valve 600 and the detection assembly 100. The reversing valve 600 has a switchable first connection state and a second connection state: in the first connection state, the reversing valve 600 connects the solution delivery assembly 400 and the solution preparation channel 500, as well as the liquid phase fluid delivery assembly 200 and the chromatographic column 300; in the second connection state, the reversing valve 600 connects the liquid phase fluid delivery assembly 200, the solution preparation channel 500, and the chromatographic column 300. The solution delivery assembly 400 is used to deliver a solution prepared from at least the sample to the solution preparation channel 500 via the reversing valve 600. The liquid phase fluid delivery assembly 200 is used to drive the solution in the solution preparation channel 500 to the chromatographic column 300 via liquid phase fluid, and to drive liquid phase fluid to flow sequentially through the reversing valve 600 and the chromatographic column 300.

[0158] In one embodiment, the test solution delivery assembly 400 includes a sampling component 410, a reaction container 420, and a sample delivery path 430. The sampling component 410 is used to draw a sample from the sample container and dispense it into the reaction container 420. The sample delivery path 430 is used to dispense the hemolysis treatment solution into the reaction container 420 and to deliver the test solution made of at least the sample and the hemolysis treatment solution in the reaction container 420 to the test solution preparation channel 500 via a reversing valve 600.

[0159] In one implementation, the sample infusion circuit 430 includes a third drive pump.

[0160] In one embodiment, the hemolysis solution includes at least one of the following liquids: a hemolytic agent containing a surfactant to dissolve and break down red blood cells in the sample; and deionized water, purified water, or distilled water that swells and breaks down red blood cells in the sample through osmotic pressure. The hemolytic agent containing a surfactant primarily dissolves red blood cells, causing them to release hemoglobin, thus processing the whole blood sample into a test solution. Deionized water, purified water, or distilled water primarily uses osmotic pressure to swell and break down red blood cells in the whole blood sample, causing them to release hemoglobin, thus also processing the whole blood sample into a test solution.

[0161] In one embodiment, when the hemolysis solution is a hemolysin containing a surfactant to dissolve and break down red blood cells in a sample, the infusion line 430 is used to draw the hemolysis solution from the hemolysin container to the reaction container 420.

[0162] The liquid chromatography analysis apparatus 10 provided in the second aspect of the present invention includes a sample delivery assembly 400, a sample preparation channel 500, a liquid phase fluid delivery assembly 200, a chromatographic column 300, a detection assembly 100, and a controller. The sample delivery assembly 400 is used to deliver a sample prepared from at least a sample to the sample preparation channel 500. The liquid phase fluid delivery assembly 200 is used to drive the sample in the sample preparation channel 500 through the chromatographic column 300 via a liquid phase fluid, so that the sample is adsorbed onto the chromatographic column 300; and to drive the liquid phase fluid through the chromatographic column 300 so that the liquid phase fluid elutes the sample adsorbed onto the chromatographic column 300, thereby forming a test solution. The detection assembly 100 is used to perform optical detection on the test solution flowing out of the chromatographic column 300 and obtain optical detection information. The controller is configured to process the optical detection information and output the detection result of the sample. The detection component 100 includes a light source 110, a light shaping component 120, a light receiving component 140, and an installation structure. The detection component 100 forms a detection channel 131, a liquid inlet 132, and a liquid outlet 133. The detection channel 131 is located within the installation structure. The liquid inlet 132 and the liquid outlet 133 are respectively connected to the detection channel 131. The liquid inlet 132 is connected to the chromatographic column 300 to allow the test liquid flowing out of the chromatographic column 300 to flow into the detection channel 131. The liquid outlet 133 is used to allow the test liquid in the detection channel 131 to flow out. The light source 110 is used to emit light; the light shaping component 120 is disposed between the light source 110 and the detection channel 131 to shape the light emitted by the light source 110 and then irradiate the test liquid in the detection channel 131; the light receiving component 140 is used to receive the light detection information formed by the light irradiated by the light shaping component 120 and transmitted through the test liquid in the detection channel 131; the mounting structure forms a mounting channel 151, and the mounting channel 151 forms an arc-shaped mounting part, which is at least used to mount the light shaping component 120, and the central axis of the mounting part, the central axis of the detection channel 131 and the optical axis of the light shaping component 120 are substantially coincident.

[0163] The liquid chromatography analysis apparatus 10 provided in the second aspect of the present invention focuses on limiting the light-shaping component 120 to an arc-shaped mounting portion in the mounting channel 151, without limiting the mounting channel 151 to forming an independent mounting component. That is, the mounting channel 151 and the detection channel 131 can also be integrally formed on the same component. Using the liquid chromatography analysis apparatus 10 provided in the second aspect of the present invention can at least improve the installation and debugging efficiency of the light-shaping component 120.

[0164] In a first embodiment of the mounting channel 151 and the detection channel 131, the detection assembly 100 further includes a flow meter 130. The flow meter 130 has a detection channel 131, a liquid inlet 132, and a liquid outlet 133. The flow meter 130 and the mounting structure are integrally formed, and the optical shaping component 120 is installed within the flow meter 130. In this embodiment, the mounting channel 151 is formed on the flow meter 130, that is, the optical shaping component 120 is nested and installed within the flow meter 130. It should be noted that, in specific applications, the optical shaping component 120 can be directly embedded and installed within the flow meter 130, or it can be indirectly embedded and installed within the flow meter 130 through one or more cylindrical components.

[0165] In a second embodiment of the mounting channel 151 and the detection channel 131, the detection assembly 100 further includes a flow meter 130. The flow meter 130 has a detection channel 131, an inlet 132, and an outlet 133. The flow meter 130 and the mounting structure are separately disposed, with at least a portion of the photoshaping component 120 installed within the mounting structure, and the mounting structure installed within the flow meter 130. In this embodiment, the mounting channel 151 is formed on a single cylindrical component. At least a portion of the photoshaping component 120 is first installed on this single cylindrical component, and then the cylindrical component is nested with the flow meter 130. It should be noted that in specific applications, the photoshaping component 120 can be directly embedded in a single cylindrical component, or it can be nested with multiple cylindrical components.

[0166] As a third embodiment of the mounting channel 151 and the detection channel 131, the detection assembly 100 further includes a flow device 130. The flow device 130 has a detection channel 131, a liquid inlet 132, and a liquid outlet 133. The flow device 130 and the mounting structure are separately disposed, and the flow device 130 and at least a portion of the light-shaping component 120 are mounted within the mounting structure. In this embodiment, the mounting channel 151 is formed on a separate cylindrical component (e.g., the first mounting component 150 described in the first aspect of the present invention), and the flow device 130 and at least a portion of the light-shaping component 120 are mounted on this separate cylindrical component. It should be noted that, in specific applications, the flow device 130 and the light-shaping component 120 can be directly embedded in the mounting channel 151, or they can be indirectly embedded in the mounting channel 151 through one or more cylindrical components.

[0167] In one implementation, the mounting structure is a single, integrally molded component.

[0168] In one embodiment, the mounting structure is an integrally formed cylindrical component, and the mounting part is a circular through hole formed inside the cylindrical component.

[0169] Alternatively, as another implementation, the mounting structure is an integrally formed open slot-shaped component, and the mounting part is an arc-shaped groove formed on one side of the open slot-shaped component.

[0170] Alternatively, as another embodiment, the mounting structure is a component integrally formed and at least partially cylindrical and at least partially open slot-shaped. The mounting channel 151 includes at least two mounting portions distributed along the axial direction of the mounting structure, wherein at least one mounting portion is a circular through hole formed in the cylindrical portion, and at least the other mounting portion is an arc-shaped groove formed on one side of the open slot-shaped portion.

[0171] Apart from the above, other parts and principles of the liquid chromatography analysis device 10 provided in the second aspect of the present invention can be referred to the liquid chromatography analysis device 10 provided in the first aspect above, and will not be described in detail here.

[0172] The liquid chromatography analysis apparatus 10 provided in the third aspect of the present invention includes a sample delivery assembly 400, a sample preparation channel 500, a liquid phase fluid delivery assembly 200, a chromatographic column 300, a detection assembly 100, and a controller. The sample delivery assembly 400 is used to deliver a sample prepared from at least a sample to the sample preparation channel 500. The liquid phase fluid delivery assembly 200 is used to drive the sample in the sample preparation channel 500 through the chromatographic column 300 via a liquid phase fluid, so that the sample is adsorbed onto the chromatographic column 300; and to drive the liquid phase fluid through the chromatographic column 300 so that the liquid phase fluid elutes the sample adsorbed onto the chromatographic column 300, thereby forming a test solution. The detection assembly 100 is used to perform optical detection on the test solution flowing out of the chromatographic column 300 and obtain optical detection information. The controller is configured to process the optical detection information and output the detection result of the sample. The detection component 100 includes a light source 110, a light shaping component 120, a flow meter 130, a light receiving component 140, and a first mounting component 150. The flow meter 130 has a detection channel 131, a liquid inlet 132, and a liquid outlet 133. The liquid inlet 132 and the liquid outlet 133 are respectively connected to the detection channel 131. The liquid inlet 132 is connected to the chromatographic column 300 to allow the test liquid flowing out of the chromatographic column 300 to flow into the detection channel 131. The liquid outlet 133 is used to allow the test liquid in the detection channel 131 to flow out. The light source 110 is used to emit light. The light shaping component 120 is disposed between the light source 110 and the flow meter 130 to shape the light emitted by the light source 110 and irradiate the test liquid in the detection channel 131. The light receiving component 140 is used to receive the light detection information formed by the light irradiated by the light shaping component 120 and transmitted through the test liquid in the detection channel 131. The first mounting component 150 has a mounting channel 151, which is used to mount at least the light shaping component 120 and the flow meter 130. The mounting channel 151 also has an arc-shaped mounting portion for mounting the light shaping component 120 and / or for mounting the flow meter 130. The light emitted by the light source 110 is shaped by the light shaping component 120 and then irradiates the test liquid in the detection channel 131. The light receiving component 140 can receive the light detection information formed by the light emitted by the light source 110, shaped by the light shaping component 120, and transmitted through the test liquid in the detection channel 131.

[0173] The liquid chromatography analysis device 10 provided in the third aspect of the present invention focuses on defining a light-shaping component 120, a flow channel 130, and an arc-shaped mounting portion installed in the mounting channel 151. Light emitted from the light source 110 is shaped by the light-shaping component 120 and then irradiates the test liquid in the detection channel 131. A light-receiving component 140 receives light detection information formed by light emitted from the light source 110, shaped by the light-shaping component 120, and transmitted through the test liquid in the detection channel 131. Using the liquid chromatography analysis device 10 provided in the second aspect of the present invention can at least improve the installation and debugging efficiency of the light-shaping component 120.

[0174] Apart from the above, other parts and principles of the liquid chromatography analysis device 10 provided in the third aspect of the present invention can be referred to the liquid chromatography analysis device 10 provided in the first and second aspects above, and will not be described in detail here.

[0175] The detection assembly 100 provided in the fourth aspect of the present invention includes a light source 110, a light shaping component 120, a flow meter 130, a light receiving component 140, and a first mounting component 150. The flow meter 130 has a detection channel 131, an inlet 132, and an outlet 133. The inlet 132 and the outlet 133 are respectively connected to the detection channel 131. The inlet 132 is connected to a chromatographic column 300 to allow the test liquid flowing out of the chromatographic column 300 to flow into the detection channel 131. The outlet 133 is used to allow the test liquid in the detection channel 131 to flow out. The light source 110 is used to emit light. The light shaping component 120 is disposed between the light source 110 and the flow meter 130. Between 30, it is used to shape the light emitted by the light source 110 and irradiate the test liquid in the detection channel 131; the light receiving component 140 is used to receive the light detection information formed by the light irradiated by the light shaping component 120 and transmitted through the test liquid in the detection channel 131; the first mounting component 150 forms a mounting channel 151, which is used to mount at least the light shaping component 120 and the flow meter 130, and the mounting channel 151 forms an arc-shaped mounting portion, which is used to mount the light shaping component 120 and / or to mount the flow meter 130, and the central axis of the mounting portion, the central axis of the detection channel 131 and the optical axis of the light shaping component 120 are substantially coincident.

[0176] Apart from the above, other parts and principles of the liquid chromatography analysis device 10 provided in the fourth aspect of the present invention can be referred to the detection component 100 in the liquid chromatography analysis device 10 provided in the first to third aspects, and will not be described in detail here.

[0177] The above description is merely a preferred embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural transformations made using the contents of the present invention's specification and drawings under the inventive concept of the present invention, or direct / indirect applications in other related technical fields, are included within the patent protection scope of the present invention.

Claims

1. A liquid chromatography analysis device, characterized in that: It includes a test solution delivery assembly, a test solution preparation channel, a liquid phase fluid delivery assembly, a chromatographic column, a detection assembly, and a controller; The test solution delivery assembly is used to deliver a test solution prepared from at least the sample to the test solution preparation channel; The liquid-phase fluid delivery assembly is used to drive the test solution in the test solution preparation channel through the chromatographic column via liquid-phase fluid, so that the test solution is adsorbed onto the chromatographic column; And for driving the liquid fluid through the chromatographic column so that the liquid fluid elutes the test solution adsorbed on the chromatographic column to form the test solution; The detection component is used to perform optical detection on the test liquid flowing out of the chromatographic column and obtain optical detection information; The controller is configured to process the light detection information and output the detection result of the sample; The detection component includes a light source, a light shaping component, a flow meter, a light receiving component, and a first mounting component. The flow device has a detection channel, an inlet, and an outlet. The inlet and the outlet are respectively connected to the detection channel. The inlet is connected to the chromatographic column to allow the test solution flowing out of the chromatographic column to flow into the detection channel. The outlet is used to allow the test solution in the detection channel to flow out. The light source is used to emit light; The light shaping component is disposed between the light source and the flow meter to shape the light emitted by the light source and then irradiate the test liquid in the detection channel. The light receiving component is used to receive the light detection information formed by the test liquid that has been irradiated by the light shaping component and passed through the test channel; The first mounting component has a mounting channel for mounting at least the optical shaping component and the flow meter. The mounting channel has an arc-shaped mounting portion for mounting the optical shaping component and / or the flow meter. The central axis of the mounting portion, the central axis of the detection channel, and the optical axis of the optical shaping component are substantially coincident.

2. The liquid chromatograph as described in claim 1, characterized in that: The central axis of the flow meter is substantially coincident with the central axis of the mounting part, the central axis of the detection channel, and the optical axis of the optical shaping component; Optionally, the mounting part is a full circle structure with an arc angle of 360°; or, the mounting part is an arc-shaped structure with an arc angle of less than 360°.

3. The liquid chromatograph as described in claim 1, characterized in that: The mounting part is an integral structure that extends continuously in an arc shape around the circumference of the mounting channel; Alternatively, the mounting portion may include at least two separate structures arranged in an arc around the mounting channel, with adjacent separate structures abutting each other or spaced apart.

4. The liquid chromatograph as described in claim 1, characterized in that: The mounting channel includes at least two mounting portions distributed axially along the first mounting component, one of the mounting portions being used to mount the flow device, and at least the other mounting portion being used to mount at least a portion of the light shaping component; And / or, the liquid chromatography analysis device further includes a support plate, and the light shaping component and the flow meter are mounted on the support plate via the first mounting component.

5. The liquid chromatograph as described in any one of claims 1 to 4, characterized in that: The first mounting component is a single, integrally molded component; Optionally, the first mounting component is an integrally formed cylindrical component, and the mounting portion is a circular through hole formed in the cylindrical component; Alternatively, the first mounting component is an integrally formed open slot-shaped component, and the mounting portion is an arc-shaped groove formed on one side of the open slot-shaped component; Alternatively, the first mounting component is an integrally formed component that is at least partially cylindrical and at least partially open slot-shaped. The mounting channel includes at least two mounting portions distributed along the axial direction of the first mounting component, wherein at least one mounting portion is a circular through hole formed in the cylindrical portion, and at least the other mounting portion is an arc-shaped groove formed on one side of the open slot-shaped portion.

6. The liquid chromatograph as described in claim 5, characterized in that: When the first mounting component is an integrally formed cylindrical component, the mounting channel is a stepped through hole arranged along the axial direction of the first mounting component, and the stepped through hole forms at least two circular through holes with different inner diameters. Alternatively, when the first mounting component is an integrally formed open slot-shaped component, the mounting channel is a stepped groove arranged along the axial direction of the first mounting component, and the stepped groove forms at least two arc-shaped grooves with different inner diameters. Alternatively, when the first mounting component is a one-piece molded component that is at least partially cylindrical and at least partially open slotted, the mounting channel includes at least one of the circular through holes and at least one arc-shaped groove with an inner diameter different from the inner diameter of the at least one of the circular through holes.

7. The liquid chromatograph as described in any one of claims 1 to 4, characterized in that: The first mounting component includes at least two sub-mounting components, each of which is at least partially nested and connected to at least one other sub-mounting component; Each of the sub-mounts has at least one mounting portion, the flower is mounted on the mounting portion of one of the sub-mounts, and at least a portion of the light-shaping component is mounted on the mounting portion of at least another sub-mount.

8. The liquid chromatograph as described in any one of claims 1 to 4, characterized in that: The light shaping component includes a collimating lens, which is disposed between the light source and the flow meter. The collimating lens is used to convert the light emitted by the light source into parallel light and then illuminate the test liquid in the detection channel. The mounting channel has a first mounting portion and a second mounting portion distributed along the axial direction of the first mounting component. The central axis of the first mounting portion and the central axis of the second mounting portion are substantially coincident. The inner diameter of the first mounting portion is larger than the inner diameter of the second mounting portion. The flow meter is mounted on the first mounting portion, and the collimating lens is mounted on the second mounting portion.

9. The liquid chromatograph as described in claim 8, characterized in that: The optical shaping component further includes an aperture stop, which is installed in the mounting channel and positioned between the collimating lens and the flow meter. The aperture stop is used to constrain the light beam that passes through the collimating lens and illuminates the detection channel. Preferably, the mounting channel further comprises a third mounting portion and a fourth mounting portion, wherein the second mounting portion, the third mounting portion, the fourth mounting portion and the first mounting portion are distributed sequentially along the axial direction of the first mounting component, and the inner diameters of the second mounting portion, the third mounting portion, the fourth mounting portion and the first mounting portion increase sequentially, and the aperture stop is mounted on the third mounting portion and the fourth mounting portion; Preferably, the collimating lens is embedded in the aperture stop at one end facing the flowmeter; Preferably, the optical component further includes an axial positioning element, which is connected to one end of the flow element and the first mounting component to perform axial positioning of the flow element. The other end of the flow element abuts against one end of the aperture stop to perform axial positioning of the aperture stop, and the other end of the aperture stop abuts against one end of the collimating lens to perform axial positioning of the collimating lens. And / or, the light shaping component further includes a first condenser lens and a second condenser lens, the flow device has a first cavity on the side facing the aperture stop, the flow device has a second cavity on the side facing away from the aperture stop, the first condenser lens is disposed in the first cavity, and the second condenser lens is disposed in the second cavity.

10. The liquid chromatograph according to any one of claims 1 to 4, characterized in that: The detection component further includes a light guide extending from the light source to the first mounting component, the light guide being used to guide the light emitted by the light source through it and then illuminate the light shaping component; The light guide component is composed of at least one of optical fiber, light guide rod, light guide tube, and light guide plate; The optical axis of the light-emitting end of the light guide is substantially coincident with the central axis of the mounting channel, the central axis of the detection channel, and the optical axis of the light-shaping component.

11. The liquid chromatograph as described in claim 10, characterized in that: A fifth mounting portion is also formed at one end of the mounting channel, and the light-emitting end of the light guide is mounted on the fifth mounting portion; Preferably, the optical shaping component includes a collimating lens and an aperture stop. The mounting channel further forms a first mounting portion, a second mounting portion, a third mounting portion, and a fourth mounting portion. The flow meter is mounted on the first mounting portion, the collimating lens is mounted on the second mounting portion, and the aperture stop is mounted on the third and fourth mounting portions. The fifth, second, third, and fourth mounting portions and the first mounting portion are distributed sequentially along the axial direction of the first mounting component.

12. The liquid chromatograph as described in any one of claims 1 to 4, characterized in that: The outer surface of the first mounting component is formed with a first clearance groove extending to the inlet of the flow device and a second clearance groove extending to the outlet of the flow device; The liquid chromatography analysis device further includes a first inlet pipe and a first outlet pipe. The first inlet pipe is connected to the inlet through the first clearance groove, and the first outlet pipe is connected to the outlet through the second clearance groove. Preferably, the first clearance groove and the second clearance groove extend to the end face of the flow device away from the light source.

13. The liquid chromatograph as described in any one of claims 1 to 4, characterized in that: The detection component further includes a second mounting component, the light receiving component is mounted on the second mounting component, and the first mounting component and the second mounting component are at least partially nested and connected to each other; Alternatively, the mounting channel may also be used to mount the optical receiving component.

14. The liquid chromatograph according to any one of claims 1 to 4, characterized in that: The light source is used to emit light of at least a first wavelength and a second wavelength; The light receiving component includes a first photodetector, a second photodetector, and a beam splitter. The photodetector, the beam splitter, and the channel are sequentially distributed along the optical axis of the light shaping component. The second photodetector is located to the side of the optical axis of the light shaping component. The beam splitter is used to split the light emitted through the channel into light of the first wavelength and light of the second wavelength, and to allow the light of the first wavelength to be transmitted to the first photodetector and to allow the light of the second wavelength to be reflected to the second photodetector. The detection component further includes a second mounting component, on which the first photodetector, the second photodetector, and the beam splitter are all mounted, and the first mounting component and the second mounting component are at least partially nested and connected to each other.

15. The liquid chromatograph as described in claim 14, characterized in that: The light source includes a single lamp body, which is used to emit light of at least the first wavelength and light of the second wavelength; Alternatively, the light source may include at least two lamp bodies, wherein at least one lamp body is used to emit light of the first wavelength and at least another lamp body is used to emit light of the second wavelength.

16. A liquid chromatography analysis apparatus, characterized in that: It includes a test solution delivery assembly, a test solution preparation channel, a liquid phase fluid delivery assembly, a chromatographic column, a detection assembly, and a controller; The test solution delivery assembly is used to deliver a test solution prepared from at least the sample to the test solution preparation channel; The liquid-phase fluid delivery assembly is used to drive the test solution in the test solution preparation channel through the chromatographic column via liquid-phase fluid, so that the test solution is adsorbed onto the chromatographic column; And for driving the liquid fluid through the chromatographic column so that the liquid fluid elutes the test solution adsorbed on the chromatographic column to form the test solution; The detection component is used to perform optical detection on the test liquid flowing out of the chromatographic column and obtain optical detection information; The controller is configured to process the light detection information and output the detection result of the sample; The detection component includes a light source, a light shaping component, a light receiving component, and an installation structure. The detection component has a detection channel, an inlet, and an outlet. The detection channel is located within the mounting structure. The inlet and outlet are respectively connected to the detection channel. The inlet is connected to the chromatographic column to allow the test solution flowing out of the chromatographic column to flow into the detection channel. The outlet is used to allow the test solution in the detection channel to flow out. The light source is used to emit light; The light shaping component is disposed between the light source and the detection channel to shape the light emitted by the light source and then irradiate the test liquid in the detection channel. The light receiving component is used to receive the light detection information formed by the test liquid that has been irradiated by the light shaping component and passed through the test channel; The mounting structure has an mounting channel, and the mounting channel has an arc-shaped mounting portion. The mounting portion is used to mount the optical shaping component. The central axis of the mounting portion, the central axis of the detection channel, and the optical axis of the optical shaping component are substantially coincident.

17. The liquid chromatograph as described in claim 16, characterized in that: The detection assembly further includes a flow meter, which has the detection channel, the liquid inlet and the liquid outlet, and the flow meter and the mounting structure are integrally formed, with the optical shaping component installed inside the flow meter; Alternatively, the detection assembly may further include a flow meter having the detection channel, the inlet, and the outlet, and the flow meter and the mounting structure are separately configured, with at least a portion of the photoshaping component installed within the mounting structure, and the mounting structure installed within the flow meter; Alternatively, the detection assembly may further include a flow meter having the detection channel, the inlet, and the outlet, and the flow meter and the mounting structure may be separately configured, with the flow meter and at least a portion of the photoshaping component mounted within the mounting structure.

18. The liquid chromatograph as described in claim 16 or 17, characterized in that: The mounting structure is a single, integrally molded component; Optionally, the mounting structure is an integrally formed cylindrical component, and the mounting portion is a circular through hole formed inside the cylindrical component; Alternatively, the mounting structure is an integrally formed open slot-shaped component, and the mounting part is an arc-shaped groove formed on one side of the open slot-shaped component; Alternatively, the mounting structure is a component integrally formed and at least partially cylindrical and at least partially open slot-shaped, the mounting channel including at least two mounting portions distributed along the axial direction of the mounting structure, wherein at least one mounting portion is a circular through hole formed in the cylindrical portion, and at least another mounting portion is an arc-shaped groove formed on one side of the open slot-shaped portion.

19. A liquid chromatography analysis apparatus, characterized in that: It includes a test solution delivery assembly, a test solution preparation channel, a liquid phase fluid delivery assembly, a chromatographic column, a detection assembly, and a controller; The test solution delivery assembly is used to deliver a test solution prepared from at least the sample to the test solution preparation channel; The liquid-phase fluid delivery assembly is used to drive the test solution in the test solution preparation channel through the chromatographic column via liquid-phase fluid, so that the test solution is adsorbed onto the chromatographic column; And for driving the liquid fluid through the chromatographic column so that the liquid fluid elutes the test solution adsorbed on the chromatographic column to form the test solution; The detection component is used to perform optical detection on the test liquid flowing out of the chromatographic column and obtain optical detection information; The controller is configured to process the light detection information and output the detection result of the sample; The detection component includes a light source, a light shaping component, a flow meter, a light receiving component, and a first mounting component. The flow device has a detection channel, an inlet, and an outlet. The inlet and the outlet are respectively connected to the detection channel. The inlet is connected to the chromatographic column to allow the test solution flowing out of the chromatographic column to flow into the detection channel. The outlet is used to allow the test solution in the detection channel to flow out. The light source is used to emit light; The light shaping component is disposed between the light source and the flow meter to shape the light emitted by the light source and then irradiate the test liquid in the detection channel. The light receiving component is used to receive the light detection information formed by the test liquid that has been irradiated by the light shaping component and passed through the test channel; The first mounting component has a mounting channel for mounting at least the optical shaping component and the flow meter, and the mounting channel has an arc-shaped mounting portion for mounting the optical shaping component and / or for mounting the flow meter; The light emitted by the light source can be shaped by the light shaping component and then irradiated onto the test liquid in the detection channel. The light receiving component can receive the light detection information formed by the light emitted by the light source, shaped by the light shaping component, and transmitted through the test liquid in the detection channel.

20. A detection component, characterized in that: It includes a light source, a light shaping component, a flow meter, a light receiving component, and a first mounting component; The flow device has a detection channel, an inlet, and an outlet. The inlet and the outlet are respectively connected to the detection channel. The inlet is connected to the chromatographic column to allow the test solution flowing out of the chromatographic column to flow into the detection channel. The outlet is used to allow the test solution in the detection channel to flow out. The light source is used to emit light; The light shaping component is disposed between the light source and the flow meter to shape the light emitted by the light source and then irradiate the test liquid in the detection channel. The light receiving component is used to receive the light detection information formed by the test liquid that has been irradiated by the light shaping component and passed through the test channel; The first mounting component has a mounting channel for mounting at least the optical shaping component and the flow meter. The mounting channel has an arc-shaped mounting portion for mounting the optical shaping component and / or the flow meter. The central axis of the mounting portion, the central axis of the detection channel, and the optical axis of the optical shaping component are substantially coincident.

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