Long-acting compound essential oil and preparation method thereof

Abstract

The application provides a long-acting compound essential oil and a preparation method thereof, and belongs to the technical field of compound essential oil.The compound essential oil composition comprises sweet orange essential oil, bergamot essential oil, lavender essential oil, ylang-ylang essential oil, melaleuca alternifolia essential oil, patchouli essential oil, cedar essential oil, vetiver essential oil and sandalwood essential oil.The application effectively solves the technical problems of short fragrance retention time, easy and rapid loss of fragrance, and unstable fragrance level of the existing essential oil composition by scientifically proportioning essential oil components with different volatile rates, achieves long-acting, stable and level fragrance retention effect, and can effectively relieve human anxiety, calm the nerves and relax the body and mind.

Description

Technical Field

[0001] This invention belongs to the field of compound essential oil technology, and in particular relates to a long-lasting fragrance compound essential oil and its preparation method. Background Technology

[0002] Plant essential oils are volatile aromatic substances extracted from the flowers, leaves, stems, roots, or fruits of plants through processes such as steam distillation, pressing, and supercritical extraction. Due to their natural gentleness, rich aroma layers, and multiple benefits including mood soothing, antibacterial and anti-inflammatory properties, and skin conditioning, they are widely used in aromatherapy, personal care, home fragrance, daily chemical products, and textile scenting. Compared to single essential oils, compound essential oils, through the scientific blending of multiple essential oils, achieve synergistic aromas and complementary effects, meeting more diverse usage needs and are currently the mainstream products on the market.

[0003] Currently, most commercially available compound essential oils are simple physical mixtures of various essential oils. In practical applications, this results in numerous insurmountable technical defects, severely limiting the product's effectiveness and application scenarios. Existing compound essential oils generally suffer from short-lasting fragrance, unstable aroma release, and easily discontinuous aroma layers. The active ingredients in essential oils are mostly small-molecule terpenes, alcohols, esters, and other volatile substances with significant differences in evaporation rates. After simple blending, the top notes evaporate and dissipate rapidly, while the base notes are difficult to release steadily and continuously. This leads to an initially overly strong and pungent aroma, followed by rapid fragrance decay in the middle and later stages. The overall fragrance duration is typically only 1-3 hours, failing to achieve long-lasting and stable fragrance retention. Users need to frequently reapply the fragrance, resulting in a poor user experience and failing to meet the market demand for long-lasting and durable fragrance.

[0004] Currently, the commonly used methods for long-lasting fragrance retention in the industry mainly involve adding traditional fixatives, using cyclodextrin encapsulation, microencapsulation, and polymer film-forming technologies. Among these, simply adding fixatives can easily alter the original natural aroma of essential oils, disrupting aroma harmony and limiting the long-lasting effect; cyclodextrin encapsulation technology has low oil loading capacity, limiting the types of essential oils it can be used with, and its sustained-release effect is poor; microencapsulation is complex and costly to produce, the capsule wall material is easily damaged, and it has poor compatibility with the essential oil system, easily leading to problems such as layering and demulsification, and it can also affect the release of the natural aroma of essential oils; polymer film-forming technology can easily lead to a stuffy aroma and poor release, and it can easily produce a sticky feeling when used, failing to balance long-lasting effect and user comfort.

[0005] Therefore, there is an urgent need for a long-lasting, naturally fragrant compound essential oil. Summary of the Invention

[0006] In view of this, one of the objectives of the present invention is to provide a compound essential oil composition with long-lasting fragrance.

[0007] A second objective of this invention is to provide a method for preparing the compound essential oil composition.

[0008] A third objective of this invention is to provide the application of the compound essential oil composition or the preparation method in the preparation of products for relieving anxiety.

[0009] To achieve the above-mentioned objectives, the present invention provides the following technical solution: A long-lasting fragrance compound essential oil composition, comprising the following ingredients by weight: 4-6 parts sweet orange essential oil, 3-5 parts bergamot essential oil, 8-12 parts lavender essential oil, 4-6 parts ylang-ylang essential oil, 2-4 parts neem essential oil, 8-10 parts patchouli essential oil, 6-8 parts cedarwood essential oil, 4-6 parts vetiver essential oil, and 6-8 parts sandalwood essential oil.

[0010] Preferably, the ingredients, by weight, include the following: 5 parts sweet orange essential oil, 4 parts bergamot essential oil, 10 parts lavender essential oil, 5 parts ylang-ylang essential oil, 3 parts clematis essential oil, 9 parts patchouli essential oil, 7 parts cedarwood essential oil, 5 parts vetiver essential oil, and 7 parts sandalwood essential oil.

[0011] The present invention also provides a method for preparing the compound essential oil composition, comprising the following steps: Weigh out each essential oil ingredient, mix them, and then stir for the first time to obtain an essential oil mixture; Sweet almond oil and glycerin are mixed to obtain a mixed solvent; Add the essential oil mixture to the mixing solvent and stir a second time to obtain the initial essential oil solution; The initial essential oil solution was aged and filtered to obtain a compound essential oil composition.

[0012] Preferably, the conditions for the first stirring include: stirring temperature of 40~45℃, stirring speed of 300~500r / min, and stirring time of 30~40min; The conditions for the second stirring are: stirring temperature of 40~45℃, stirring speed of 400~600r / min, and stirring time of 25~35min.

[0013] Preferably, sweet almond oil and glycerin are mixed in a mass ratio of 2:1.

[0014] Preferably, the volume ratio of the essential oil mixture to the mixed solvent is 2:3~4.

[0015] Preferably, the dripping rate of the essential oil mixture into the mixed solvent is 1~2 mL / min.

[0016] Preferably, the aging temperature is 20~25℃ and the aging time is 24~36h.

[0017] The present invention also provides the application of the compound essential oil composition or the preparation method in the preparation of products for relieving anxiety.

[0018] Preferably, the product is used in the following ways: applied directly to the skin, placed in an essential oil diffuser for diffusion, and inhaled locally.

[0019] Compared with the prior art, the present invention has the following beneficial effects: This invention provides a long-lasting, fragrance-enhancing compound essential oil composition. By scientifically proportioning essential oil components with different evaporation rates, a gradient evaporation system of "top notes - middle notes - base notes" is constructed, effectively solving the technical problems of short fragrance duration, rapid aroma dissipation, and unstable aroma layers in existing essential oil compositions. Specifically, sweet orange and bergamot essential oils, as top notes, are mainly composed of monoterpenes and have a fast evaporation rate, quickly releasing a fresh and pleasant initial aroma. Lavender, ylang-ylang, and neem oils, as middle notes, are mainly composed of esters and sesquiterpenes and have a moderate evaporation rate, effectively supporting the top notes and connecting with the base notes, stabilizing the overall aroma structure. Patchouli, vetiver, sandalwood, and cedarwood essential oils serve as base notes (base notes). The fixative component is rich in high-boiling-point sesquiterpenes and sesquiterpenes such as patchouli alcohol, sandalwood alcohol, vetiverol, and cedrol. With a large molecular weight and extremely low volatility, it can form a weak adsorption effect on the skin or carrier surface, significantly slowing down the volatilization and oxidation rate of the overall essential oil composition, reducing aroma loss. Simultaneously, it synergistically forms a stable aroma-slow-release system with the top and middle notes, greatly extending the fragrance duration and achieving a long-lasting, stable, and layered fragrance effect, thus enhancing the user experience. This invention's essential oil composition can effectively relieve anxiety, calm the mind, and relax the body and mind, solving the technical problems of existing essential oil compositions having a single soothing effect and lacking specificity. Detailed Implementation

[0020] This invention provides a long-lasting fragrance compound essential oil composition, comprising the following ingredients by weight: 4-6 parts sweet orange essential oil, 3-5 parts bergamot essential oil, 8-12 parts lavender essential oil, 4-6 parts ylang-ylang essential oil, 2-4 parts neem oil, 8-10 parts patchouli essential oil, 6-8 parts cedarwood essential oil, 4-6 parts vetiver essential oil, and 6-8 parts sandalwood essential oil. Preferably, by weight, it comprises the following ingredients: 5 parts sweet orange essential oil, 4 parts bergamot essential oil, 10 parts lavender essential oil, 5 parts ylang-ylang essential oil, 3 parts neem oil, 9 parts patchouli essential oil, 7 parts cedarwood essential oil, 5 parts vetiver essential oil, and 7 parts sandalwood essential oil. All essential oils in this invention are commercially available, and the purity of each essential oil is ≥95%.

[0021] In the compound essential oil composition of this invention, sweet orange essential oil and bergamot essential oil are the top notes. Sweet orange essential oil has a sweet orange scent, which can dispel tension and stress, and improve insomnia caused by anxiety. Bergamot essential oil is a volatile essential oil extracted by pressing from the peel of the Italian bergamot plant (Rutaceae family). It is emerald green in color and has a fresh and elegant aroma that blends orange, lemon, and floral notes. Bergamot essential oil can improve skin problems such as acne and eczema by balancing sebum secretion, relieve physiological symptoms such as urinary tract inflammation and indigestion, and also has a psychological regulating function of calming emotions and relieving anxiety. Sweet orange and bergamot are citrus essential oils dominated by monoterpenes, which evaporate quickly and have a refreshing aroma, responsible for the initial pleasant feeling and avoiding a dull fragrance.

[0022] In the compound essential oil composition of this invention, lavender essential oil, ylang-ylang essential oil, and melaleuca viridiflora essential oil are the middle notes. Lavender essential oil is a volatile aromatic substance extracted by steam distillation from the flowers of plants in the genus *Lavender* (Lamiaceae family). It has antibacterial, anti-inflammatory, sedative, and wound-healing effects, and can relieve anxiety and improve sleep by regulating neurotransmitters. Ylang-ylang essential oil is a natural essential oil extracted by steam distillation from fresh ylang-ylang flowers, and has multiple effects such as soothing emotions, regulating endocrine function, and skin care. Melaleuca viridiflora essential oil is extracted from the branches and leaves of *Melaleuca viridiflora* (also known as broadleaf melaleuca). The essential oil is pale yellow to light green, with a fresh, slightly herbal and woody aroma, and has good antibacterial and antiviral effects. The lavender, ylang-ylang, and melaleuca viridiflora essential oils have a moderate evaporation rate, serving to bridge the top and bottom notes and stabilize the aroma structure.

[0023] In the compound essential oil composition of this invention, patchouli essential oil, cedarwood essential oil, vetiver essential oil, and sandalwood essential oil are the base notes. Patchouli essential oil is a dark, viscous liquid extracted from the leaves of patchouli (a plant in the Lamiaceae family) through distillation. It is characterized by its earthy and woody aroma. Its core components include patchouli alcohol and patchoulene, which have antibacterial, anti-inflammatory, and skin-repairing effects, and are widely used in skincare, mood regulation, and insect repellent applications. Cedarwood essential oil is a volatile aromatic substance extracted from the flowers, leaves, stems, roots, or fruits of cedar through steam distillation, expression, cold maceration, or solvent extraction. It has a delicate woody fragrance and significant anti-inflammatory effects. It also possesses antibacterial, astringent, diuretic, emollient, expectorant, antifungal, and tonic properties. Furthermore, the calming effect of cedarwood can soothe nervous tension and anxiety. Vetiver essential oil is extracted from the roots of the vetiver plant, a member of the grass family. It has a rich lemon scent with a unique spicy aroma and is known as the "oil of tranquility" due to its excellent calming effects. Sandalwood essential oil is a pale yellow to brown liquid made from the heartwood of Indian sandalwood trees over 30 years old, produced through distillation or subcritical low-temperature extraction. It contains active ingredients such as santalol and has a sweet, woody aroma. Its core function is to promote skin cell regeneration and metabolism, improve aging, dry, and dehydrated skin, and fade scars and fine lines. It also has antibacterial, anti-inflammatory, and sebum-balancing effects. Patchouli, cedarwood, vetiver, and sandalwood essential oils are the core fixative components. With large molecular weights, high boiling points, and extremely slow evaporation, they can firmly lock in the overall fragrance, prolonging its lasting effect. They also form weak interactions with the skin's stratum corneum and fabric fibers, slowing down the overall evaporation rate and achieving a long-lasting scent, preventing rapid dissipation.

[0024] The compound essential oil composition provided by this invention achieves long-lasting and stable fragrance by constructing a gradient evaporation system of top, middle, and base notes.

[0025] The present invention also provides a method for preparing the compound essential oil composition, comprising the following steps: weighing each essential oil raw material, mixing and stirring for the first time to obtain an essential oil mixture; mixing sweet almond oil and glycerin to obtain a mixed solvent; adding the essential oil mixture to the mixed solvent and stirring for the second time to obtain a preliminary essential oil solution; aging the preliminary essential oil solution and filtering to obtain the compound essential oil composition.

[0026] In the preparation method of the present invention, the temperature of the first stirring is preferably 40-45℃, more preferably 42℃; the stirring speed of the first stirring is preferably 300-500 r / min, more preferably 400 r / min; and the stirring time of the first stirring is preferably 30-40 min, more preferably 35 min. The temperature of the second stirring is preferably 40-45℃, more preferably 42℃; the stirring speed of the second stirring is preferably 400-600 r / min, more preferably 500 r / min; and the stirring time of the second stirring is preferably 25-35 min, more preferably 30 min. Sweet almond oil and glycerin are preferably mixed at a mass ratio of 2:1; the glycerin is preferably vegetable glycerin. The volume ratio of the essential oil mixture to the mixed solvent is preferably 2:3-4, and in some examples it can be 2:3 or 2:4. The dropping rate of the essential oil mixture into the mixed solvent is preferably 1-2 mL / min, and in some examples it can be 1 mL / min or 2 mL / min. The aging temperature is preferably 20~25℃, more preferably 23℃; the aging time is preferably 24~36h, more preferably 28~32h, and even more preferably 30h.

[0027] The preparation method of this invention allows essential oils with different volatilization rates to be fully miscible and uniformly dispersed, forming a stable single-phase system, resulting in a smoother and longer-lasting aroma release. Aging allows the components of the compound essential oil to form a stable associated structure through intermolecular forces. The low-volatility fixative components naturally encapsulate and slow-release the high-volatility components, making the aroma of the compound essential oil composition more rounded, mellow, and harmonious, with a more uniform volatilization rate and a significantly longer fragrance retention time.

[0028] The present invention also provides the application of the compound essential oil composition or the preparation method in the preparation of products for relieving anxiety, wherein the method of using the product includes: applying it directly to the skin, placing it in an essential oil diffuser for diffusion, and local inhalation.

[0029] The compound essential oil composition of this invention has a pleasant and invigorating top note, a calming and relaxing middle note, and a soothing and calming base note. It has significant effects in soothing and relaxing, calming and calming the mind, relieving anxiety, and calming the body and mind. The aroma is released slowly and has a long-lasting fragrance. After use, it can effectively relieve tension and irritability, stabilize emotions, and relax the body and mind. The overall fragrance is mild and harmonious, non-irritating, and has excellent aroma stability and physiological soothing effect.

[0030] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0031] Example 1 A long-lasting fragrance compound essential oil composition, comprising the following ingredients by weight: 5 parts sweet orange essential oil, 4 parts bergamot essential oil, 10 parts lavender essential oil, 5 parts ylang-ylang essential oil, 3 parts clematis essential oil, 9 parts patchouli essential oil, 7 parts cedarwood essential oil, 5 parts vetiver essential oil, and 7 parts sandalwood essential oil.

[0032] The preparation method is as follows: Weigh each essential oil ingredient, mix them, and then stir for the first time (42℃, 400r / min, 35min) to obtain an essential oil mixture; Sweet almond oil and glycerin are mixed in a mass ratio of 2:1 to obtain a mixed solvent; The essential oil mixture was added dropwise to the mixed solvent at a rate of 1 mL / min, with a volume ratio of 2:3 between the essential oil mixture and the mixed solvent. The mixture was stirred a second time (42℃, 500 r / min, 30 min) to obtain the initial essential oil solution. The initial essential oil solution was aged at 23°C for 30 hours and then filtered using a 0.22μm microporous membrane to obtain a compound essential oil composition.

[0033] Example 2 A long-lasting fragrance compound essential oil composition, comprising the following ingredients by weight: Sweet orange essential oil 4 parts, bergamot essential oil 5 parts, lavender essential oil 11 parts, ylang-ylang essential oil 4 parts, neem essential oil 4 parts, patchouli essential oil 10 parts, cedarwood essential oil 8 parts, vetiver essential oil 4 parts, sandalwood essential oil 6 parts.

[0034] The preparation method is the same as in Example 1.

[0035] Example 3 A long-lasting fragrance compound essential oil composition, comprising the following ingredients by weight: 6 parts sweet orange essential oil, 3 parts bergamot essential oil, 8 parts lavender essential oil, 6 parts ylang-ylang essential oil, 2 parts clematis essential oil, 8 parts patchouli essential oil, 6 parts cedarwood essential oil, 6 parts vetiver essential oil, and 8 parts sandalwood essential oil.

[0036] The preparation method is the same as in Example 1.

[0037] Comparative Example 1 A long-lasting fragrance compound essential oil composition, comprising the following ingredients by weight: 5 parts sweet orange essential oil, 4 parts bergamot essential oil, 9 parts patchouli essential oil, 7 parts cedarwood essential oil, 5 parts vetiver essential oil, and 7 parts sandalwood essential oil.

[0038] The preparation method is the same as in Example 1.

[0039] Comparative Example 2 A long-lasting fragrance compound essential oil composition, comprising the following ingredients by weight: 5 parts sweet orange essential oil, 4 parts bergamot essential oil, 10 parts lavender essential oil, 5 parts ylang-ylang essential oil, and 3 parts chebula essential oil.

[0040] The preparation method is the same as in Example 1.

[0041] Comparative Example 3 A long-lasting fragrance compound essential oil composition, comprising the following ingredients by weight: 5 parts sweet orange essential oil, 4 parts bergamot essential oil, 10 parts lavender essential oil, 5 parts ylang-ylang essential oil, 3 parts neem essential oil, 9 parts patchouli essential oil, 7 parts cedarwood essential oil, 5 parts benzoin essential oil, and 7 parts frankincense essential oil.

[0042] The preparation method is the same as in Example 1.

[0043] Comparative Example 4 A long-lasting fragrance compound essential oil composition, comprising the following ingredients by weight: 5 parts sweet orange essential oil, 4 parts bergamot essential oil, 10 parts lavender essential oil, 5 parts ylang-ylang essential oil, 3 parts clematis essential oil, 5 parts patchouli essential oil, 3 parts cedarwood essential oil, 3 parts vetiver essential oil, and 3 parts sandalwood essential oil.

[0044] The preparation method is the same as in Example 1.

[0045] Example 4 The lasting power of different blended essential oils.

[0046] 1. Test samples: Compound essential oil compositions prepared in Examples 1 to 3 and Comparative Examples 1 to 4. Each group of samples was tested three times, and the average value was taken.

[0047] 2. Test conditions: temperature 25±2℃, relative humidity 50±5%, environment with no wind, no odor, and no direct sunlight.

[0048] 3. Testing Method: 3.1 Skin fragrance retention time: The forearm of the subjects was wiped with 75% medical ethanol and allowed to air dry for 5 minutes. 10 μL of the test sample was applied to the inner side of the forearm, and the time was recorded immediately. For the first 2 hours, the subject smelled the fragrance every 5 minutes, and after 2 hours, every 10 minutes. After the fragrance significantly weakened, the time was changed to every 30 minutes. The smelling distance was 3-5 cm, and the smelling time for each time did not exceed 2 seconds. The fragrance retention was considered to have ended when no characteristic fragrance could be detected after 3 consecutive smellings. The results are shown in Table 1.

[0049] 3.2 Scent Retention Time of Scent Test Strips: A standard scent test strip measuring 15 cm × 0.5 cm was used, with a sample volume of 20 μL. The time was recorded immediately after application. For the first 2 hours, the scent was smelled every 5 minutes; after 2 hours, it was smelled every 10 minutes. Once the aroma significantly weakened, the time was changed to every 30 minutes. The smelling distance was 3–5 cm, and each smelling session did not exceed 2 seconds. The scent retention was considered complete when no characteristic aroma could be detected after three consecutive smellings. The results are shown in Table 1.

[0050] Table 1. Scent retention time of different compound essential oil compositions

[0051] The compound essential oil provided by this invention has a fresh and bright top note that enhances a pleasant feeling, a soft and soothing middle note with a natural fragrance transition, and a rich and stable base note with woody and earthy notes, which can significantly prolong the lasting fragrance. The compound essential oil composition in Comparative Example 1 omits lavender, ylang-ylang, and neem oils, resulting in a lighter initial volatility followed by a heavier base note. While the overall lasting fragrance is further extended, the middle note completely disappears, creating a two-tiered structure of fresh fruity and rich woody notes without a smooth transition. The overall fragrance is no longer rounded and soft, lacking a gentle and soothing effect. Comparative Example 2 lacks low-volatility fixing components. After the middle note fragrance dissipates, there is no subsequent fragrance support, and the overall lasting fragrance quickly fades to odorless. It can only provide temporary and mild emotional comfort, without long-lasting calming, soothing, or sleep-inducing effects. Comparative Example 3, where vetiver oil was replaced with benzoin oil and sandalwood oil with frankincense oil, showed insufficient lingering base notes, rapid scent decay, and an inability to provide long-lasting soothing effects. Comparative Example 4, lacking sufficient low-volatility, high-adhesion components, failed to effectively lock in the fragrance, resulting in rapid dissipation after the mid-notes and a complete loss of lingering base notes. Lacking a sufficient woody, resinous base, the fragrance's adhesion to skin or test strips was significantly weakened, providing only moderate-duration, mild soothing effects and lacking long-lasting calming, soothing, and sleep-inducing effects.

[0052] Example 5 Compound essential oils can relieve anxiety.

[0053] 1. Experimental materials.

[0054] 1.1 Experimental animals: SPF-grade ICR male mice, 6-8 weeks old, weighing 18-22g, were acclimatized for 7 days. The housing environment was 22±2℃, 50%±5% humidity, with a 12h light-dark cycle, and free access to food and water.

[0055] 1.2 Test substance: The compound essential oil composition provided in Example 1.

[0056] 1.3 Positive control: Diazepam injection (specification: 2mg / mL).

[0057] 2. Experimental grouping and drug administration.

[0058] Mice were randomly divided into 4 groups of 10 mice each. The grouping and administration methods are as follows: (1) Blank control group: Normal feeding, daily inhalation (nebulization) of physiological saline (0.1 mL / animal, once a day); (2) Anxiety model group: The chronic unpredictable mild stress (CUMS) model was established. After successful modeling, the animals were given 0.1 mL of physiological saline (once a day) via inhalation (nebulization). (3) Positive control group: After CUMS modeling, diazepam 1.5 mg / kg was injected intraperitoneally daily; (4) Compound essential oil group: After CUMS modeling, the compound essential oil composition of Example 1 was administered by inhalation (nebulization) once a day.

[0059] The modeling period was 28 days, and the drug administration period was synchronized for 7 days. Behavioral tests were performed 60 minutes after the last drug administration.

[0060] 3. Experimental methods and detection indicators.

[0061] 3.1 Anxiety model establishment: The CUMS method was used to randomly apply the following stimuli for 28 consecutive days (one type per day, without repetition): isolation for 24 hours, wet cage (with wet filter paper at the bottom of the cage) for 24 hours, tail clamping (1 minute), fasting and water restriction for 12 hours, and day-night reversal for 24 hours.

[0062] 3.2 Behavioral Testing: The Elevated Cross Maze (EPM) and Open Field Test (OFT) were used, and relevant indicators were recorded using a behavioral video analysis system. The results are shown in Tables 2 and 3.

[0063] 3.4 Statistical methods: SPSS 26.0 software was used for analysis. Quantitative data are expressed as mean ± standard deviation (x±s). One-way ANOVA was used for comparisons between groups, and LSD-t test was used for pairwise comparisons. P<0.05 was considered statistically significant.

[0064] 4. Behavioral test results.

[0065] Table 2 Effects of compound essential oils on elevated cruciate maze behavior in CUMS model mice (x±s, n=10)

[0066] Note: Compared with the blank control group, △△P<0.01; compared with the anxiety model group, P<0.01.

[0067] Table 3 Effects of compound essential oils on open field behavior in CUMS model mice (x±s, n=10)

[0068] Note: Compared with the blank control group, △△P<0.01; compared with the anxiety model group, P<0.01.

[0069] As shown in Table 2, compared with the blank control group, the percentage of time spent in open arms and the percentage of times the mice entered open arms in the CUMS-induced anxiety model were significantly reduced, while the total number of times the mice entered arms remained unchanged. This indicates that the model mice exhibited significant anxiety-like behavior and showed significant avoidance of open environments, indicating successful establishment of the anxiety model. The positive control group (diazepam) significantly reversed the above behavioral changes, with both the percentage of time spent in open arms and the percentage of entry arms significantly increasing, confirming the reliability of the detection system used in this experiment.

[0070] Compared with the anxiety model group, the percentage of time mice spent in open arms and the percentage of times they entered open arms were significantly increased in the compound essential oil group of this invention, and all indicators were close to the level of the blank control group. This indicates that the compound essential oil of this invention can significantly improve the anxiety-like behavior of CUMS model mice and enhance their willingness to explore open environments and their ability to handle approach-avoidance conflicts.

[0071] Table 3 shows that the model group mice had significantly shorter dwell time in the central area of ​​the open field and shorter total movement distance than the blank control group, and the number of stress-induced fecal pellets was significantly increased, further confirming the presence of anxiety. The positive control group showed significant improvement in the above indicators, proving the effectiveness of the experimental system.

[0072] The open field behavior of the compound essential oil group of this invention was significantly improved: the central zone dwell time recovered to 78.67±10.12s, and the total movement distance increased to 1723.45±155.78cm, which were significantly different from the model group, but not significantly different from the blank control group and the positive control group; at the same time, the number of fecal particles decreased to 2.80±0.89, which was significantly lower than that of the model group. The above results indicate that the compound essential oil of this invention can effectively alleviate the stress response of mice and restore their normal exploratory activity and neural homeostasis.

[0073] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A long-lasting fragrance-retaining compound essential oil composition, characterized by, The ingredients, by weight, include the following: 4-6 parts sweet orange essential oil, 3-5 parts bergamot essential oil, 8-12 parts lavender essential oil, 4-6 parts ylang-ylang essential oil, 2-4 parts neem essential oil, 8-10 parts patchouli essential oil, 6-8 parts cedarwood essential oil, 4-6 parts vetiver essential oil, and 6-8 parts sandalwood essential oil.

2. The complex essential oil composition according to claim 1, characterized by, The ingredients, by weight, include the following: 5 parts sweet orange essential oil, 4 parts bergamot essential oil, 10 parts lavender essential oil, 5 parts ylang-ylang essential oil, 3 parts neem essential oil, 9 parts patchouli essential oil, 7 parts cedarwood essential oil, 5 parts vetiver essential oil, and 7 parts sandalwood essential oil.

3. The method of claim 1 or 2, wherein the method comprises the steps of: Includes the following steps: Weigh out each essential oil ingredient, mix them, and then stir for the first time to obtain an essential oil mixture; Sweet almond oil and glycerin are mixed to obtain a mixed solvent; Add the essential oil mixture to the mixing solvent and stir a second time to obtain the initial essential oil solution; The initial essential oil solution was aged and filtered to obtain a compound essential oil composition.

4. The production method according to claim 3, characterized by, The conditions for the first stirring include: stirring temperature of 40~45℃, stirring speed of 300~500r / min, and stirring time of 30~40min; The conditions for the second stirring are: stirring temperature of 40~45℃, stirring speed of 400~600r / min, and stirring time of 25~35min.

5. The preparation method according to claim 3, characterized in that, Sweet almond oil and glycerin are mixed in a mass ratio of 2:

1.

6. The preparation method according to claim 3, characterized in that, The volume ratio of the essential oil mixture to the mixed solvent is 2:3~4.

7. The preparation method according to claim 3, characterized in that, The essential oil mixture is added to the mixed solvent at a rate of 1-2 mL / min.

8. The preparation method according to claim 3, characterized in that, The aging temperature is 20~25℃; the aging time is 24~36h.

9. The use of the compound essential oil composition according to claim 1 or 2 or the preparation method according to any one of claims 3 to 8 in the preparation of products for relieving anxiety.

10. The application according to claim 9, characterized in that, The product can be used in the following ways: applied directly to the skin, diffused using an essential oil diffuser, or inhaled locally.

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