Preparation method and application of photo-induced nanoscale enzyme

CN122321858APending Publication Date: 2026-07-03HENAN UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HENAN UNIV OF SCI & TECH
Filing Date
2026-04-07
Publication Date
2026-07-03

AI Technical Summary

Technical Problem

Existing sensors exhibit small signal variation and limited sensitivity when detecting phosphate (Pi) in agricultural irrigation water. Furthermore, when nanozymes are combined with organic photosensitizers or noble metal nanomaterials, they suffer from low interfacial electron transfer efficiency and poor photostability.

Method used

A photo-induced nanozyme composed of nitrogen-carbon quantum dots (NCDs) and copper-iron layered double hydroxide (CuFe-LDH) was prepared by electrostatic adsorption. The photoresponsive properties were used to enhance the activity of the nanozyme, thereby achieving highly sensitive detection of Pi.

Benefits of technology

It achieves rapid and accurate detection of Pi, with NCDs-LDH nanozyme activity enhanced by 5.7 times, exhibiting high sensitivity and specificity, and can effectively identify Pi in complex aquatic environments.

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Abstract

This invention discloses a method for preparing photoinduced nanozymes and their applications, relating to the field of nanocomposite materials technology. The method includes: synthesizing nitrogen-carbon quantum dots (NCDs) using citric acid and urea as raw materials via microwave heating, followed by purification to obtain purified NCDs; preparing a precursor solution using copper nitrate and ferric nitrate, adding an alkaline solution, and synthesizing copper-iron layered double hydroxide (CuFe-LDH) via a hydrothermal reaction; then combining NCDs and CuFe-LDH in a sodium hydroxide solution via electrostatic adsorption and one-step stirring, followed by separation, washing, and drying to obtain the NCDs-LDH nanocomposite material. This invention provides mild preparation conditions, is simple and rapid to operate, and the obtained material can be used to construct colorimetric sensors to achieve highly sensitive and specific detection of phosphate ions (Pi).
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