A porcine CD40L trimer protein, a recombinant Bacillus subtilis expressing this protein, and its applications.
By designing specific fusion protein sequences in Bacillus subtilis, efficient soluble expression and secretion of porcine CD40L trimer protein were achieved, solving the expression problem in Escherichia coli systems, improving the immunization effect of porcine vaccines, and simplifying the production process.
CN122325580APending Publication Date: 2026-07-03SHANDONG AGRICULTURAL UNIVERSITY
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Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SHANDONG AGRICULTURAL UNIVERSITY
- Filing Date
- 2026-06-05
- Publication Date
- 2026-07-03
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Figure CN122325580A_ABST
Abstract
This invention discloses a porcine CD40L trimer protein, a recombinant Bacillus subtilis strain expressing this protein, and its applications, belonging to the fields of microbial genetic engineering and livestock and poultry biological products technology. This invention utilizes Bacillus subtilis WB800N as a host and pHT43 as a vector to successfully construct a recombinant strain that efficiently secretes and expresses porcine CD40L trimer protein. Soluble expression of the porcine CD40L trimer protein was achieved. The amino acid sequence of the porcine CD40L trimer protein expressed by the recombinant strain is shown in SEQ ID NO:1. Immunological activity verification using this porcine CD40L trimer protein revealed that the CD40L trimer protein expressed in this invention can simultaneously activate humoral and cellular immunity, possessing practical application value as a novel vaccine adjuvant.
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