A single-nitrogen-source regulation medium for white rot fungi, and a preparation method and application thereof

Abstract

This invention relates to the field of microbial technology. It provides a culture medium for regulating a single nitrogen source in white-rot fungi, its preparation method, and its applications. The culture medium uses water as a solution and further includes potassium dihydrogen phosphate, disodium hydrogen phosphate, a carbon source, magnesium sulfate heptahydrate, manganese sulfate tetrahydrate, zinc sulfate heptahydrate, copper sulfate pentahydrate, calcium chloride heptahydrate, ferrous sulfate heptahydrate, and a nitrogen source. The core advantage of the culture medium and preparation method designed in this invention lies in the precise control of the type of nitrogen source, effectively eliminating interference from multiple nitrogen sources. This provides a dedicated culture medium for studying the physiological metabolism, molecular mechanisms, and biosynthesis of white-rot fungi under specific nitrogen source environments, and is widely applicable to transcriptional, protein and metabolic regulation, as well as biodegradation and synthetic biology in white-rot fungi.

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