An anti-aging traditional Chinese medicine composition and a preparation method thereof

Abstract

An anti-aging traditional Chinese medicine composition and its preparation method belong to the field of traditional Chinese medicine technology. The composition consists of the following components by weight: 3-60 parts of Polygonatum sibiricum, 1-70 parts of Astragalus membranaceus, 2-40 parts of Lycium barbarum, and 1-40 parts of Ganoderma lucidum spore powder. The method is as follows: soak the raw materials except for Ganoderma lucidum spore powder; decoct the raw materials 1-3 times; combine the decoctions obtained after several decoctions and concentrate them to obtain concentrated decoction; prepare the concentrated decoction into freeze-dried powder; add Ganoderma lucidum spore powder to the freeze-dried powder and stir evenly. The pharmacodynamic research results of the anti-aging of the present invention are as follows: (1) The test drug can significantly prolong the average lifespan of naturally aging nematodes by 30%, which is better than Erjingwan and the Western medicine NMN; (2) improve exercise, feeding ability and nervous system function; (3) improve antioxidant capacity: reduce the fluorescence intensity of ROS and lipofuscin, and improve the survival rate of nematodes after H2O2 stress stimulation; (4) improve telomerase activity; (5) improve cognitive dysfunction and learning and memory ability.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine technology, specifically relating to a traditional Chinese medicine composition for improving aging and its preparation method. Background Technology

[0002] Aging is a systemic biological process with exceptionally complex mechanisms. Fourteen aging markers include: genomic instability, telomere loss, epigenetic alterations, loss of protein homeostasis, macroautophagy dysfunction, nutrient sensing dysregulation, mitochondrial dysfunction, cellular senescence, stem cell depletion, altered intercellular communication, chronic inflammation and dysbiosis, extracellular matrix changes, and psychosocial isolation. Among these 14 markers, telomerase activity plays a central and pivotal role. Telomerase gene mutations trigger premature aging and shortened lifespan. Telomerase activity directly determines telomere dynamics, affects stem cell regeneration capacity, tissue repair, and the overall aging process.

[0003] Currently, clinical interventions for aging mainly include metformin (a hypoglycemic drug), rapamycin (a drug that inhibits the mTOR pathway), nicotinamide mononucleotide (NMN) and NR (N-nucleotides), drugs that clear senescent cells such as dasatinib and quercetin, anti-inflammatory drugs such as probiotics, aspirin, and ibuprofen, and autophagy activator spermidine. While these drugs have some efficacy, they also have many drawbacks. Because anti-aging drugs generally suffer from problems such as "single target and significant side effects," there is an urgent clinical need for safe, effective, and multi-target synergistic innovative drugs to open up new avenues for anti-aging treatments.

[0004] Traditional Chinese medicine (TCM) boasts thousands of years of human experience and is characterized by its multi-component, multi-target synergistic, and holistic approach to disease treatment, demonstrating unique advantages in the field of anti-aging. From a TCM perspective, the core theory of aging is the theory of kidney deficiency leading to aging. Kidney deficiency is the root of aging; kidney essence, as the material basis for maintaining the body's form and spirit, is the source of aging. The *Medical Orthodox Transmission* states, "Abundant kidney qi prolongs life, while deficient kidney qi leads to premature death." The *Essential Readings for Physicians* proposes, "Nourishing life begins with nourishing the kidneys; nourishing the kidneys is nourishing life." Based on the TCM theory of "treating disease before it manifests," the *Essential Prescriptions of the Golden Chamber*, based on the theory of the five elements' mutual generation and restraint, recognizes that the spleen influences the kidneys, thus strengthening the spleen should be prioritized. The kidneys are the foundation of innate constitution, while the spleen is the foundation of acquired constitution, the source of qi and blood. The *Mirror of Regulating Deficiency* proposes an anti-aging theory of "tonifying the spleen first, then tonifying the kidneys as the primary focus." Therefore, based on TCM anti-aging theories, kidney-tonifying, essence-replenishing, spleen-strengthening, and qi-boosting TCM herbs are the preferred choice for anti-aging drugs.

[0005] Traditional Chinese medicine, which is both food and medicine, has the dual attributes of "dietary therapy" and "anti-aging health care," and has no side effects with long-term use, meeting consumers' demand for natural and safe anti-aging health products.

[0006] Erjing Wan is a classic anti-aging formula, first recorded in *Shengji Zonglu* (Comprehensive Records of Sacred Relief), Volume 198. Composed of Polygonatum sibiricum and Lycium barbarum, it has the effects of invigorating qi and consolidating essence, protecting the dantian (lower abdomen), and promoting blood circulation and maintaining youthful appearance. Polygonatum sibiricum enters the spleen, lung, and kidney meridians, and has the effects of "tonifying qi and nourishing yin, strengthening the spleen, moistening the lungs, and benefiting the kidneys and replenishing essence," often used to delay aging. Lycium barbarum enters the liver and kidney meridians, and can "nourish the liver and kidneys, benefit essence and improve eyesight." The combination of the two works synergistically by "tonifying the innate (kidney) and nourishing the acquired (spleen)," simultaneously tonifying the liver, strengthening the spleen, and nourishing the kidneys, thus improving symptoms such as premature aging and weakness. However, long-term use of the rich and nourishing Polygonatum sibiricum and Lycium barbarum can easily cause stomach upset and dampness, requiring the addition of spleen-strengthening and stomach-regulating herbs, as well as spirit-regulating herbs, to achieve the goal of strengthening the body's resistance and harmonizing yin and yang. Modern research has confirmed that both herbs in Erjing Wan can delay aging by regulating telomerase activity. From a research translation perspective, to target aging, it is necessary to add traditional Chinese medicines that are both food and medicine to Erjing Pill to target and regulate telomerase activity, so as to improve the targeting of the drug's efficacy and better meet the needs of "precise intervention in aging" in modern research.

[0007] Although there are many types of traditional Chinese medicine products for anti-aging on the market, the efficacy and safety of most of them still need further verification. Therefore, developing a food-grade medicine product that is effective, safe, and easy to use is of great significance for improving aging symptoms and enhancing the quality of life for the elderly. Summary of the Invention

[0008] To address the problem of how to develop an anti-aging traditional Chinese medicine product with definite efficacy and high safety in the existing technology, the present invention provides an anti-aging traditional Chinese medicine composition and its preparation method.

[0009] This invention comprises the following traditional Chinese medicine components: Polygonatum sibiricum, Astragalus membranaceus, Lycium barbarum, and Ganoderma lucidum spore powder. This combination of traditional Chinese medicine is based on the core theory of "preventive treatment" in Traditional Chinese Medicine. Building upon the classic TCM formula "Erjing Wan" for treating premature aging, and adhering to the principles of tonifying the kidneys and replenishing essence, strengthening the spleen and invigorating qi, it scientifically combines two medicinal and edible herbs with clearly defined quantitative ratios and optimized processes. Polygonatum sibiricum is the principal herb, Astragalus membranaceus is the assistant herb, Lycium barbarum is the adjuvant herb, and Ganoderma lucidum spore powder is the guiding herb. This embodies the classic anti-aging theory of "prioritizing spleen tonification and focusing on kidney tonification" proposed in the Ming Dynasty text *Lixu Yuanjian*. By activating telomerase activity, it prolongs lifespan and significantly improves various symptoms of aging. Its effects are superior to the classic formula "Erjing Wan," and it is more effective, lower in cost, and safer than current Western anti-aging drugs such as nicotinamide mononucleotide (NMN), making it suitable for large-scale application.

[0010] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0011] An anti-aging traditional Chinese medicine composition, wherein the composition comprises, by weight, the following components: 3-60 parts Polygonatum sibiricum, 1-70 parts Astragalus membranaceus, 2-40 parts Lycium barbarum, and 1-40 parts Ganoderma lucidum spore powder.

[0012] A method for preparing the above-mentioned anti-aging traditional Chinese medicine composition, the method comprising the following steps:

[0013] Step 1: Soak all ingredients except for Ganoderma lucidum spore powder;

[0014] Step 2: Boil the ingredients 1-3 times;

[0015] Step 3: Combine the decoctions obtained from several decoctions and concentrate them to obtain a concentrated decoction;

[0016] Step 4: Prepare the concentrated drug solution into lyophilized powder;

[0017] Step 5: Add the freeze-dried powder to the Ganoderma lucidum spore powder and stir well. For clinical use, Ganoderma lucidum spore powder is best taken by dissolving in water or dissolving in water; when used in compound prescriptions, it must be added last and never decocted together throughout the entire process. This is because its active ingredient, Ganoderma lucidum triterpenoids, has poor stability and is more easily degraded at higher temperatures.

[0018] Furthermore, in step one, the soaking time is 30-60 minutes.

[0019] Furthermore, in step two, the mass ratio of the soaking liquid to the raw materials is 12.5:1 for each decoction.

[0020] Furthermore, in step two, first bring the raw materials and all the soaking liquid to a boil over high heat, then reduce the heat to low and continue simmering for 30-60 minutes. After simmering, separate the liquid and dregs, add water to the dregs for the next simmering, and each simmering time is 30-60 minutes.

[0021] The beneficial effects of this invention compared to the prior art are as follows: The pharmacodynamic research results of this invention on anti-aging are as follows: (1) The test drug can significantly prolong the average lifespan of naturally aging nematodes by 30%, which is significantly better than Erjingwan and the Western medicine NMN; (2) It improves the movement, feeding and nervous system function of naturally aging nematodes; (3) It improves antioxidant capacity: reduces the fluorescence intensity of ROS and lipofuscin in nematodes, and improves the survival rate of nematodes after H2O2 stress stimulation; (4) It increases the activity of telomerase, telomerase reverse transcriptase and its genes; (5) It improves cognitive dysfunction and learning and memory ability in mice. In summary, this invention significantly enhances telomerase activity, prolongs the lifespan of aging nematodes, improves various aging symptoms, is of the same origin as food and medicine, has high safety, and is suitable for widespread application. Detailed Implementation

[0022] The technical solution of the present invention will be further described below with reference to the embodiments, but it is not limited thereto. Any modifications or equivalent substitutions to the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention should be covered within the protection scope of the present invention.

[0023] Example 1: An anti-aging traditional Chinese medicine composition, consisting of the following components: 3-5 parts Polygonatum sibiricum, 1-4 parts Astragalus membranaceus, 2-4 parts Lycium barbarum, and 1-4 parts Ganoderma lucidum spore powder.

[0024] Example 2: An anti-aging traditional Chinese medicine composition, consisting of the following components: 6-14 parts Polygonatum sibiricum, 5-9 parts Astragalus membranaceus, 5-9 parts Lycium barbarum, and 5-8 parts Ganoderma lucidum spore powder.

[0025] Example 3: An anti-aging traditional Chinese medicine composition, consisting of the following components: 15-29 parts Polygonatum sibiricum, 10-19 parts Astragalus membranaceus, 10-14 parts Lycium barbarum, and 9-19 parts Ganoderma lucidum spore powder.

[0026] Example 4: An anti-aging traditional Chinese medicine composition, consisting of the following components: 30-44 parts Polygonatum sibiricum, 20-39 parts Astragalus membranaceus, 15-24 parts Lycium barbarum, and 20-24 parts Ganoderma lucidum spore powder.

[0027] Example 5: An anti-aging traditional Chinese medicine composition, consisting of the following components: 45-60 parts Polygonatum sibiricum, 40-70 parts Astragalus membranaceus, 25-40 parts Lycium barbarum, and 25-40 parts Ganoderma lucidum spore powder.

[0028] The above composition was prepared by the following method:

[0029] Step 1: Soak all ingredients except Ganoderma lucidum spore powder for 30-60 minutes;

[0030] Step 2: Boil the raw materials twice; each time, the mass ratio of the soaking liquid to the raw materials is 12.5:1; first, bring the raw materials and all the soaking liquid to a boil over high heat, then turn the high heat to low heat and continue to boil for 40-50 minutes. After boiling, separate the liquid and the dregs, add water to the dregs and boil again. Each boiling time is 40-50 minutes.

[0031] Step 3: Combine the decoctions obtained from several decoctions and concentrate them to obtain a concentrated decoction;

[0032] Step 4: Prepare the concentrated drug solution into lyophilized powder;

[0033] Step 5: Add the freeze-dried powder to the Ganoderma lucidum spore powder and stir well. For clinical use, Ganoderma lucidum spore powder is best taken by dissolving in water or dissolving in water; when used in compound prescriptions, it must be added last and never decocted together throughout the entire process. This is because its active ingredient, Ganoderma lucidum triterpenoids, has poor stability and is more easily degraded at higher temperatures.

[0034] The traditional Chinese medicine composition obtained in Example 4 was used as the low-dose group of the test drug, and the traditional Chinese medicine composition obtained in Example 5 was used as the high-dose group of the test drug. The following experiments were conducted:

[0035] I. Experimental Methods

[0036] Nematode lifespan experiments and behavioral observations

[0037] Nematodes were transferred to culture dishes containing two dose groups of the test drug (1 mg / mL and 2 mg / mL, denoted as WL and WH), M9 buffer (blank control group), two dose groups of a classic formula (1 mg / mL and 2 mg / mL, denoted as EJ-L and EJ-H, denoted as positive control group for traditional Chinese medicine), and 1 μg / mL NMN (positive control group for Western medicine) (150 nematodes per group; n=150), and cultured in a 20℃ biological incubator. During the reproduction period, nematodes were transferred to new plates every other day at room temperature to prevent interference from hatching embryos. The number of surviving nematodes in each dish was recorded daily until all nematodes died. Nematodes were considered dead when they did not respond to repeated poking and showed no pharyngeal pumping. On days 0, 3, 6, 9, 12, and 15 of culture, 15 nematodes were randomly selected from each group, and the frequency of pharyngeal pumping, head shaking, and body bending was observed within 20 seconds at room temperature.

[0038] Detection of lipofuscin in nematodes

[0039] After incubating nematodes with the test drug, a classic formula, and NMN for 5 days (20 nematodes per plate), they were placed on 2% agarose plates and anesthetized with 10 mM levamisole. Images were captured using a fluorescence microscope, and fluorescence intensity was calculated using ImageJ software.

[0040] Detection of ROS fluorescence in nematodes

[0041] After incubating nematodes with the test drug, the classic formula, and NMN for 5 days (20 nematodes per dish), the mixture was transferred to 100 μL of DCFH-DA and incubated at room temperature in the dark for 2 h. ROS levels were detected by fluorescence microscopy.

[0042] Nematode oxidative stress experiment

[0043] After incubating the test drug, the classic formula, and NMN with nematodes for 5 days (20 nematodes per plate), the mixture was transferred to NGM plates containing 50 μL of 3% H2O2. After 5 hours, the survival rate of the nematodes was measured to assess the antioxidant stress resistance of the compound.

[0044] qRT-PCR

[0045] After incubating nematodes with the test drug, classic formula, and NMN for 48 h (at least 10,000 nematodes per dish), total RNA was extracted from nematodes or mouse tissues using the trizol method. The RNA content and quality were determined using a NanoDrop ultra-micro spectrophotometer. The extracted RNA was transcribed into cDNA using a ReverTra Ace qPCR RT kit. The nematode trt-1 gene was detected, with nematode β-actin selected as an internal control. Primer sequences are shown in Table 1. qRT-PCR was performed using cDNA ChamQ Universal SYBR qPCRMaster Mix (Q711, Vazyme) and primers. Primers were provided by Kumei Biotechnology. Amplification and melting curves were analyzed. (Ref. 2) -ΔΔCt The relative expression level of gene mRNA can be calculated using this method.

[0046] Table 1 Primer Sequences

[0047]

[0048] ELISA

[0049] After incubating nematodes with the test drug, a classic formula, and NMN for 48 hours (at least 10,000 nematodes per dish), the nematodes were washed with M9 buffer at 5000 rpm for 1 min. This process was repeated 2-3 times. The nematode pellet was then ground, sonicated, centrifuged at 12000 rpm, and the supernatant was collected. The nematode telomerase reverse transcriptase (TERT) and telomerase (TE) were detected using an ELISA kit.

[0050] Laboratory animals and grouping

[0051] Kunming mice (KM, 30-35g), SPF grade, male, 56 in total. The mice were housed in a barrier system environment with a room temperature of 20-26℃, humidity of 40%-70%, ventilation of 10-15 times / hour with 1000% fresh air, and 12 hours of light. Experimental groups: blank control group (C), aging model group (M), low-dose treatment group of test drug (WL), high-dose treatment group of test drug (WH), low-dose group of classic prescription (EJ-L), high-dose group of classic prescription (EJ-H), and metformin (MET) positive control group. Animal experiment ethics approval number: IRB3133724

[0052] Animal model preparation and administration methods

[0053] D-galactose-induced aging model in mice: After one week of acclimatization, 56 mice were randomly divided into 7 groups of 8 mice each. Except for group C, all groups of mice were subcutaneously injected with 300 mg / kg D-galactose daily for 45 consecutive days to establish an aging model (M). The blank control group (C) was given an equal volume of physiological saline; the treatment groups were administered by gavage the following drugs: low-dose treatment group (WL) 13 g / kg, high-dose treatment group (WH) 26 g / kg, low-dose treatment group (EJ-L) 9 g / kg, high-dose treatment group (EJ-H) 18 g / kg, and metformin 400 mg / kg, respectively, for 45 consecutive days.

[0054] New object recognition experiment

[0055] The Novel Object Recognition (NOR) test consists of two phases: a training phase and a testing phase. On the first day, during the training phase, two identical objects are placed equidistantly within a recognition box. The mouse is placed in the center of the box and allowed 5 minutes to explore. On the second day, during the testing phase, one of the familiar objects is replaced with a new object. The new object has the same height and volume as the familiar object but a different shape and appearance. The mouse is then placed back in the box and allowed 5 minutes to explore again. Supermaze video tracking software records the time and number of times the mouse explores each object. The mouse's recognition index is calculated using the formula: Novel object exploration time / (Novel object exploration time + Familiar object exploration time) × 100%, Novel object exploration count / (Novel object exploration count + Familiar object exploration count) × 100%.

[0056] Barnes Maze Experiment

[0057] The Barnes maze test is used to assess spatial memory. The platform is a circular maze with a diameter of 910 cm and 20 holes. Before the formal test, mice were acclimatized to the environment and then trained to freely explore the maze for 180 seconds until they reached an escape hole. Mice were trained twice daily for five consecutive days prior to the formal test. Each test was recorded by a camera mounted above the platform, and Supermaze video tracking software was used to record the mouse's exploration trajectory, latency, and total distance. Latency: the time it takes for the mouse to find the escape hole; Total distance: the distance the mouse travels to find the escape hole.

[0058] Data are expressed as mean ± SEM. Statistical analysis was performed using GraphPad Prism 10.1.2 software, employing t-tests or one-way ANOVA. p < 0.05 was considered statistically significant.

[0059] II. Experimental Results

[0060] 1. The tested drug can prolong the lifespan of naturally aging nematodes.

[0061] Mean survival time (MSG) results showed that, compared with the blank control group, the low-dose and high-dose groups of the test drug were prolonged by 23.10% and 30.19%, respectively; the low-dose and high-dose groups of the Erjingwan group were prolonged by 17.51% and 20.46%, respectively; and NMN was prolonged by 25.70%. Median survival time (MSG) results showed that, compared with the blank control group, the low-dose and high-dose groups of the test drug were prolonged by 17.50% and 37.50%, respectively; the low-dose and high-dose groups of the Erjingwan group were prolonged by 15.00% and 15.00%, respectively; and NMN was prolonged by 25.00%. The results of the maximum survival time showed that the longest survival time of nematodes in the blank group was 24.67 days. Compared with the blank control group, the low-dose and high-dose groups of the test drug were prolonged by 25.68% and 29.73%, respectively. The low-dose and high-dose groups of the Erjingwan group were prolonged by 21.62% and 24.32%, respectively, and NMN was prolonged by 22.97%.

[0062] Table 2. Effects of the test drugs on the lifespan of naturally aging nematodes (mean ± standard deviation)

[0063]

[0064] Note: Compared with group C, p < 0.001.

[0065] 2. Behavioral manifestations of the investigational drug in improving aging

[0066] Behavioral results of nematodes showed that on days 3, 12, and 15, the number of twists in naturally aging nematodes were 11.07, 5.67, and 4.13, respectively; the number of head shakes were 21.67, 15.67, and 13.93, respectively; and the pharyngeal pump frequency was 66.20, 39.33, and 37.53, respectively. After intervention with low and high doses of the test drug, the number of body twists increased by 50.12% and 66.34%, respectively; the number of head shakes increased by 45.94% and 52.19%, respectively; and the pharyngeal pump frequency increased by 46.36% and 54.01%, respectively. In the low-dose and high-dose groups of the Erjingwan group, the number of body twisting movements was 4.8 and 5.67, respectively; the number of head shaking movements was 16.73 and 18.60, respectively; and the pharyngeal pump frequency was 52.87 and 56.93, respectively. The number of head shaking movements increased by 16.22% and 37.29%, respectively; and the pharyngeal pump frequency increased by 20.10% and 33.52%, respectively. The pharyngeal pump frequency also increased by 40.87% and 51.69%, respectively. After NMN intervention, the number of body twisting movements increased by 19.37%, the number of head shaking movements increased by 23.98%, and the pharyngeal pump frequency increased by 55.42%.

[0067] Table 3. Effects of the test drugs on the aging behavior of nematodes (mean ± standard deviation)

[0068]

[0069] Note: Compared with group C, p < 0.001; compared with group NMN, p < 0.001.

[0070] 3. The test drug enhances the antioxidant capacity of nematodes.

[0071] After low- and high-dose intervention with the test drug, the fluorescence intensity of lipofuscin from naturally aging nematodes decreased by 19.28% and 15.47%, respectively, while NMN intervention reduced it by 23.44%; the low- and high-dose groups of the Erjingwan group reduced it by 26.60% and 20.97%, respectively.

[0072] After low and high doses of the test drug, the fluorescence intensity of reactive oxygen species (ROS) in naturally aging nematodes decreased by 16.05% and 18.40%, respectively, and decreased by 21.33% after NMN intervention; the low and high dose groups of the Erjingwan group decreased by 16.72% and 19.02%, respectively.

[0073] After intervention with low-dose and high-dose of the test drug, low-dose and high-dose of the Erjingwan group, and NMN, the survival rates of nematodes after H2O2 stress stimulation were increased by 112.50%, 175.00%, 100.00%, 125.00%, and 162.50%, respectively.

[0074] Table 4 Antioxidant effects of the test drugs (mean ± standard deviation)

[0075]

[0076] Note: Compared with group C, p < 0.05; Compared with group C, p < 0.01; Compared with group C, p < 0.001.

[0077] 4. The test drug increased telomerase activity in naturally aging nematodes.

[0078] Compared with the control group, the telomerase (TE) activity in nematodes increased by 38.85% and 51.35% respectively after intervention in the low- and high-dose groups of the test drug, while the low- and high-dose groups of the Erjingwan group increased by 37.06% and 44.14% respectively; all were statistically significant. The NMN group increased by 22.08%, which was not statistically significant.

[0079] Compared with the control group, after intervention with low and high doses of the test drug, the homologous gene of nematode telomerase reverse transcriptase (TRT) was significantly increased. -1) The activity was increased by 128.59% and 265.10% respectively. The low and high dose groups of the Erjingwan group were increased by 215.54% and 216.18% respectively. After NMN intervention, the activity was increased by 202.60%.

[0080] Compared with the control group, the activity of telomerase reverse transcriptase (TERT) in nematodes was upregulated by 32.81% and 69.85% after intervention in the low- and high-dose groups of the test drug, respectively. The activity was upregulated by 44.78% and 25.15% in the low- and high-dose groups of the Erjingwan group, respectively. After NMN intervention, the activity was upregulated by 62.60%.

[0081] Table 5. Effects of the test drug on telomerase, telomerase reverse transcriptase and its genes in naturally aging nematodes.

[0082] (mean ± standard deviation)

[0083]

[0084] Note: Compared with group C, p < 0.05; Compared with group C, p < 0.01; Compared with group C, p < 0.001. # Compared with group NMN, p < 0.05.

[0085] The data from the nematode experiments mentioned above are all related to anti-oxidation and delaying aging. The experimental data presented below are related to improving learning and memory abilities and delaying aging by alleviating cognitive impairment.

[0086] 5. The test drug improved cognitive dysfunction in aging mice.

[0087] The novel object recognition test is the gold standard for detecting cognitive impairment in mice. The novel object recognition index was 65.49% in the normal control group and 46.84% in the aging model group, indicating cognitive impairment. Intervention with low- and high-dose groups of the test drug improved the index by 25.47% and 30.89%, respectively; intervention with low- and high-dose groups of the classic formula improved the index by 23.08% and 29.31%, respectively; and intervention with metformin (MET) improved the index by 25.02%.

[0088] The novel object exploration time recognition index of normal control mice was 70.80%, while it decreased to 48.38% in the aging model group, indicating cognitive impairment. Intervention with low- and high-dose groups of the test drugs improved the index by 37.08% and 38.98%, respectively; intervention with low- and high-dose groups of the classic formula improved the index by 30.94% and 32.37%, respectively; and intervention with metformin (MET) improved the index by 33.18%. These findings suggest that all of these treatments have a role in improving cognitive impairment in aging mice.

[0089] Table 6. Effects of the test drugs on D-galactose-induced cognitive function in aging mice (mean ± standard deviation)

[0090]

[0091] Note: Compared with Group C, p < 0.05; p < 0.01; p < 0.001, compared with group M, # p < 0.05; ## p < 0.01; ### p < 0.001.

[0092] 6. The test drug improved the learning and memory abilities of aging mice.

[0093] The Barnes maze experiment showed that aging model mice exhibited significantly increased latency and total distance in exploring the target area, suggesting a decline in spatial learning and memory abilities. After intervention with low- and high-dose groups of the tested drugs, the latency decreased by 16.65% and 26.78%, respectively, and the total distance decreased by 22.73% and 28.55%, respectively. After intervention with low- and high-dose groups of the classic formula, the latency decreased by 21.23% and 29.22%, respectively, and the total distance decreased by 19.19% and 21.01%, respectively. Metformin (MET) reduced the latency by 32.38% and the total distance by 30.76%. These findings suggest that both methods can improve learning and memory abilities.

[0094] Table 7. Effects of the test drugs on learning and memory abilities in D-galactose-induced aging mice (mean ± standard deviation)

[0095]

[0096] Note: Compared with Group C, p < 0.05; p < 0.01; p < 0.001, compared with group M, # p < 0.05; ## p < 0.01; ### p < 0.001.

Claims

1. A traditional Chinese medicine composition for anti-aging, characterized in that: The composition comprises, by weight, the following components: 3-60 parts Polygonatum sibiricum, 1-70 parts Astragalus membranaceus, 2-40 parts Lycium barbarum, and 1-40 parts Ganoderma lucidum spore powder.

2. A method for preparing the anti-aging traditional Chinese medicine composition according to claim 1, characterized in that: The method includes the following steps: Step 1: Soak all ingredients except for Ganoderma lucidum spore powder; Step 2: Boil the ingredients 1-3 times; Step 3: Combine the decoctions obtained from several decoctions and concentrate them to obtain a concentrated decoction; Step 4: Prepare the concentrated drug solution into lyophilized powder; Step 5: Add the freeze-dried powder to the Ganoderma lucidum spore powder and stir well.

3. The method for preparing an anti-aging traditional Chinese medicine composition according to claim 2, characterized in that: In step one, the soaking time is 30-60 minutes.

4. The method for preparing an anti-aging traditional Chinese medicine composition according to claim 2, characterized in that: In step two, the mass ratio of the soaking liquid to the raw materials is 12.5:1 for each decoction.

5. The method for preparing an anti-aging traditional Chinese medicine composition according to claim 2, characterized in that: In step two, first bring the raw materials and all the soaking liquid to a boil over high heat, then reduce the heat to low and continue simmering for 30-60 minutes. After simmering, separate the liquid and dregs, add water to the dregs and simmer for the next time. Each simmering time is 30-60 minutes.

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