A stem cell activity detection culture device for treating diabetes

Abstract

The application discloses a kind of stem cell activity detection culture equipment for treating diabetes, it is related to stem cell culture technical field, including incubator, the incubator inside is provided with culture dish, the culture dish below is provided with alternating magnetic field generator, the inside of the culture dish is placed with stem cell and culture solution, the culture solution belongs to electrolyte solution, alternating current is generated in culture dish when the alternating magnetic field generator starts.This application is separated by the setting of partition ring and piston ring, cooperates alternating magnetic field generator, realizes the separation of normal cell and dead stem cell under the action of centrifugal force, monitoring mechanism cooperates piston ring, detects the cell in the edge of culture dish, recycles dead stem cell using inactivation space, observer can clearly understand the approximate situation of stem cell cultivation in culture dish, the setting of observation mechanism, and cooperate with protective ball and alternating magnetic field generator, reduce stem cell stacking, improve the observation effect of stem cell for researchers.

Description

Technical Field

[0001] This invention relates to the field of stem cell culture technology, specifically to a stem cell activity detection and culture device for the treatment of diabetes. Background Technology

[0002] Type 2 diabetes mellitus (T2DM) is a complex chronic metabolic disease, its core being insulin resistance and β-cell dysfunction under hyperinsulinemia. Studies have found that decreased peripheral tissue sensitivity to insulin leads to compensatory β-cell proliferation and excessive insulin secretion to maintain blood glucose levels. However, this compensatory capacity is limited. Under the combined effects of long-term hyperglycemia, high free fatty acid levels (lipotoxicity), chronic inflammation, and other factors, β-cell function gradually deteriorates and may even undergo apoptosis, ultimately triggering a full-blown diabetes outbreak. Furthermore, microvascular complications are a significant complication of diabetes. Insulin affects peripheral organs by regulating endothelial cell function and peripheral vascular pathways, while hyperglycemia continuously attacks the microvascular system through multiple biochemical pathways, leading to pathological changes such as superficialization and increased vascular permeability.

[0003] Current research explores the treatment of diabetes through metabolic reprogramming of mesenchymal stem cells. During this research, stem cell cultivation is a primary concern for researchers. Existing stem cell cultivation equipment focuses mainly on the cultivation process itself, with little attention paid to observation during the cultivation period. Current methods often rely on visual observation or microscopic examination of extracted solutions. Both of these methods inevitably expose the culture dish to the external environment, which can negatively impact the final cultivation results. Summary of the Invention

[0004] The purpose of this invention is to provide a stem cell activity detection and culture device for the treatment of diabetes, so as to solve the problems mentioned in the background art.

[0005] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a stem cell activity detection and culture device for treating diabetes, including a culture box, a culture dish is arranged inside the culture box, an alternating magnetic field generator is arranged below the culture dish, stem cells and culture medium are placed inside the culture dish, the culture medium is an electrolyte solution, and an alternating current is generated in the culture dish when the alternating magnetic field generator is started. An observation mechanism is provided at the top of the incubator, located directly above the culture dish, and is used to observe the survival status of stem cells in the culture dish.

[0006] Furthermore, the interior of the culture dish is provided with a partition ring, which divides the interior space of the culture dish into two parts. The space inside the partition ring is the survival space, and the space between the partition ring and the culture dish is the inactivation space. The lower end of the partition ring has a trapezoidal vertical cross-section, and the lower end surface of the partition ring does not contact the lower side wall inside the culture dish. A monitoring mechanism is provided on the lower inner side of the partition ring, which is used to monitor the survival status of stem cells near the lower inner side of the partition ring.

[0007] Furthermore, a piston ring is provided between the partition ring and the culture dish. The piston ring is located in the deactivation space. The lower surface of the piston ring is in contact with the outer wall of the partition ring and the inner wall of the culture dish. The upper outer wall size of the piston ring matches the outer wall size of the partition ring and the inner wall size of the culture dish. The piston ring is provided with a control mechanism at its upper end. The piston ring is connected to the incubator through the control mechanism. The control mechanism is used to control the piston ring to move up and down relative to the culture dish. The control mechanism changes the containment space of the deactivation space through the piston ring. The control mechanism is connected to the monitoring mechanism.

[0008] Furthermore, the monitoring mechanism includes a flow channel and electrodes. The flow channel is located on the inner side below the isolation ring and at the connection between the survival space and the inactivation space. Multiple sets of flow channels are provided and are distributed in a ring within the isolation ring. Each set of flow channels has two opposing electrodes.

[0009] Furthermore, the observation mechanism includes an observation mirror installed above the culture dish inside the incubator, with protective balls distributed in a ring below the observation mirror; The outer wall of the observation mirror is coated with an anti-stick coating, the corners of the observation mirror are rounded and chamfered, the lowest point of the observation mirror is a protective ball, and the observation mirror is connected to the incubator through another set of control mechanisms.

[0010] Furthermore, the observation mirror has an airflow channel inside, the protective ball has an opening below it, and the output end of the airflow channel is an opening.

[0011] Furthermore, a slide rail is provided below the alternating magnetic field generator. The alternating magnetic field generator is connected to the incubator through the third set of control mechanisms. The alternating magnetic field generator is slidably connected to the slide rail. The third set of control mechanisms is used to change the relative position of the alternating magnetic field generator and the culture dish.

[0012] Furthermore, the control mechanism includes an electrically controlled spring and a limiting sleeve, the limiting sleeve being disposed inside the electrically controlled spring, and the electrically controlled spring being electrically connected to the output power supply.

[0013] Compared with the prior art, the beneficial effects achieved by the present invention are: The use of a partition ring and piston ring, along with an alternating magnetic field generator, drives the culture medium in the culture dish, avoiding the movement of the culture medium by physical objects and reducing damage to stem cells. At the same time, by taking advantage of the difference in cell membrane integrity between normal stem cells and dead stem cells, the alternating magnetic field further accelerates the dead stem cells, causing them to move in a vortex manner within the culture dish. Under the action of centrifugal force, the separation of normal cells and dead stem cells is achieved. The monitoring agency, in conjunction with the piston ring, detects cells at the edge of the culture dish and uses the inactivation space to recover dead stem cells, ensuring that the area inside the partition ring is mainly composed of normal stem cells. By observing the volume changes of the inactivation space, the observer can clearly understand the general situation of stem cell cultivation in the culture dish. The observation mechanism is designed so that the eyepiece of the observation mirror is located on the outside of the incubator, allowing researchers to directly observe the stem cells during their cultivation. In addition, the protective ball and alternating magnetic field generator reduce stem cell stacking and improve the researchers' observation of the stem cells. Attached Figure Description

[0014] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used together with the embodiments of the invention to explain the invention and do not constitute a limitation thereof. In the drawings: Figure 1 This is a three-dimensional structural diagram of the incubator mechanism of the present invention; Figure 2 This is a three-dimensional structural diagram of the petri dish and the mechanism below it according to the present invention; Figure 3 This is a three-dimensional structural diagram of the petri dish and the mechanism below it according to the present invention; Figure 4 This is a schematic diagram of the bottom three-dimensional structure of the monitoring mechanism of the present invention; Figure 5 This is the invention Figure 2 Enlarged structural diagram at point A in the middle; Figure 6 This is the invention Figure 4 Enlarged structural diagram at point B; Figure 7 This is a top-view full-section structural diagram of the monitoring mechanism of the present invention.

[0015] In the diagram: 1. Incubator; 2. Petri dish; 201. Partition ring; 202. Piston ring; 3. Alternating magnetic field generator; 301. Slide rail; 4. Observation mechanism; 401. Observation mirror; 402. Protective ball; 403. Opening; 5. Control mechanism; 501. Electrically controlled spring; 502. Limiting sleeve; 6. Monitoring mechanism; 601. Flow channel; 602. Electrode. Detailed Implementation

[0016] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0017] Please see Figures 1-7 The present invention provides a technical solution: a stem cell activity detection and culture device for treating diabetes, including a culture box 1, a culture dish 2 is arranged inside the culture box 1, an alternating magnetic field generator 3 is arranged below the culture dish 2, stem cells and culture medium are placed inside the culture dish 2, the culture medium is an electrolyte solution, and an alternating current is generated in the culture dish 2 when the alternating magnetic field generator 3 is started. An observation mechanism 4 is installed inside the upper part of the incubator 1. The observation mechanism 4 is located directly above the culture dish 2 and is used to observe the survival status of stem cells in the culture dish 2. The stem cells to be cultured are placed in a culture dish 2, and then the culture dish 2 is placed above the alternating magnetic field generator 3 in the incubator 1. The alternating magnetic field generator 3 can generate an alternating current during stem cell culture. The culture dish 2 contains a culture medium containing a large number of ions to balance the osmotic pressure on both sides of the cell membrane. Under the action of the alternating magnetic field, an alternating current is formed in the culture medium. Under the action of the Lorentz force, the ions move in the solution, thereby enabling the ions to drive the movement of the stem cells inside the culture medium. Through magnetic convection, the various components in the solution are mixed with each other, while ensuring that the stem cells coexist in the culture medium of the same concentration. At the same time, the incubator 1 is equipped with an industry-standard temperature control mechanism and sterilization mechanism to ensure that the incubator 1 is in a sterile state and that the stem cells survive at a suitable temperature. The observation mechanism 4 is set up to facilitate the observation of the stem cells at various growth stages during the stem cell growth period.

[0018] See Figures 1-4 The interior of the culture dish 2 is provided with a partition ring 201, which divides the interior space of the culture dish 2 into two parts. The space inside the partition ring 201 is the survival space, and the space between the partition ring 201 and the culture dish 2 is the inactivation space. The lower end of the partition ring 201 has a trapezoidal vertical cross section, and the lower end surface of the partition ring 201 does not contact the lower side wall inside the culture dish 2. A monitoring mechanism 6 is provided on the inner side of the partition ring 201. The monitoring mechanism 6 is used to monitor the survival status of stem cells near the inner side of the partition ring 201. The main function of the partition ring 201 is to divide the internal space of the culture dish 2 into two spaces: a survival space and a deactivation space. The lower ends of the survival space and the deactivation space are connected. Under the action of the alternating magnetic field, the solution in the culture dish 2 will form a vortex. At the same time, some stem cells will die during the culture period. After the stem cells die, the cell membrane is damaged, and the ions inside the cell membrane will slowly leak out. The cell membrane of normal stem cells has a certain insulating effect. Therefore, after the cell membrane is damaged, the liquid inside the cell membrane and the culture medium in the culture dish 2 provide a basis for electrical connection. The slow leakage of ions from the dead stem cells will cause ion clusters to appear on the outside of the dead stem cells. Under the combined action of the alternating magnetic field and alternating current, the culture medium and the dead stem cells will be subjected to the Lorentz force. Since the dead stem cells are located in the culture medium, the swimming speed of the dead stem cells is greater than the swimming speed of the normal stem cells carried by the culture medium. There is a speed difference between the normal stem cells and the dead stem cells. The upper part of the partition ring 201 is a cylinder, and the lower part of the partition ring 201 is a truncated cone with a vertical cross-section of trapezoidal shape. Normal stem cells and dead stem cells move in a circular motion within the partition ring 201. Under the action of centrifugal force, due to the eddy current motion of the culture medium and the annular shape of the lower part of the partition ring 201, the proportion of dead stem cells is higher in the ring near the inner wall of the partition ring 201, while the proportion of normal stem cells is higher on the side near the central axis of the partition ring 201. In addition, the lower diameter of the partition ring 201 is larger than the upper diameter. Under the action of centrifugal force, dead stem cells mainly gather in the lower part of the partition ring 201, that is, at the junction of the living space and the inactivation space. Therefore, under the action of the alternating magnetic field generator 3, the dead stem cells move from the living space to the inactivation space through the gap between the partition ring 201 and the culture dish 2. A monitoring mechanism 6 is installed at the connection between the living space and the inactivation space. The main function of the monitoring mechanism 6 is to monitor the stem cells passing through the gap between the partition ring 201 and the culture dish 2, and to monitor whether the stem cells passing through are in an inactivated state.

[0019] See Figures 1-4 A piston ring 202 is provided between the partition ring 201 and the culture dish 2. The piston ring 202 is located in the deactivation space. The lower surface of the piston ring 202 is in contact with the outer wall of the partition ring 201 and the inner wall of the culture dish 2. The upper outer wall size of the piston ring 202 matches the outer wall size of the partition ring 201 and the inner wall size of the culture dish 2. A control mechanism 5 is provided at the upper end of the piston ring 202. The piston ring 202 is connected to the incubator 1 through the control mechanism 5. The control mechanism 5 is used to control the piston ring 202 to rise and fall relative to the culture dish 2. The control mechanism 5 changes the containment space of the deactivation space through the piston ring 202. The control mechanism 5 is connected to the monitoring mechanism 6. Under the influence of the velocity difference between normal stem cells and dead stem cells, dead stem cells will actively move towards the side where the inactivation space is located. A piston ring 202 is installed within the inactivation space, and the outer wall size of the piston ring 202 matches the structure of the inactivation space. A control mechanism 5 is installed at the upper end of the piston ring 202, which can control the piston ring 202 to rise or fall relative to the culture dish 2. Changes in the relative position of the piston ring 202 cause a synchronous change in the volume of the inactivation space, thereby controlling the number of stem cells entering the inactivation space. A monitoring mechanism 6 is electrically connected to the control mechanism 5 through a control center within the incubator 1. The monitoring mechanism 6 feeds back the detected data to the control center, which then makes a judgment based on the feedback data and sends control information to the control mechanism 5. That is, when the monitoring mechanism 6 detects... Once a high proportion of dead stem cells are present, the control center will raise the piston ring 202 via the control mechanism 5, thereby increasing the volume of the inactivation space to accommodate more dead cells. This continues until the monitoring mechanism 6 detects that the proportion of nearby normal stem cells begins to rise. At this point, the piston ring 202 will stop rising, and the volume of the inactivation space will be fixed. Normal stem cells in the living space will find it difficult to pass through the gap between the partition ring 201 and the culture dish 2 into the inactivation space because the inactivation space is already saturated, there is no longer a pressure difference between the inactivation space and the living space, and the flow rate of normal stem cells is relatively slower than that of dead stem cells. Under the action of centrifugal force, the relatively high-speed moving dead stem cells will exert a pushing force on the normal stem cells, with the direction of the pushing force being the position of the central axis of the partition ring 201.

[0020] See Figure 7 The monitoring mechanism 6 includes a flow channel 601 and electrodes 602. The flow channel 601 is located on the inner side of the isolation ring 201 and below. The flow channel 601 is located at the connection between the survival space and the inactivation space. There are multiple sets of flow channels 601, which are arranged in a ring within the isolation ring 201. Each set of flow channels 601 has two opposing electrodes 602 inside. The flow channel 601 is located on the inner lower side of the partition ring 201, at the connection between the survival space and the inactivation space. When stem cells move, they will move in a circular motion around the central axis of the partition ring 201. Multiple sets of flow channels 601 are arranged on the inner lower side of the partition ring 201. The horizontal cross-section of the flow channel 601 is arc-shaped, and the outer wall size of the flow channel 601 matches the inner wall size of the partition ring 201. During the movement of stem cells, some stem cells will enter the flow channel 601. Two electrodes 602 are arranged opposite each other inside the flow channel 601. The electrodes 602 are connected to a power source. When stem cells pass between the two electrodes 602... When the resistance between electrodes 602 increases, i.e., the Coulter effect, a resistance recorder is set in the circuit where electrodes 602 are located. When dead stem cells pass between two electrodes 602, the insulation effect of the cell membrane fails due to cell membrane damage. However, during the passage of dead stem cells, the proportion of culture medium between the two electrodes 602 is relatively small. Therefore, the time between dead stem cells and electrodes 602 is greater than the resistance of the culture medium and much less than the resistance of normal stem cells. By detecting the stem cells passing through the gap between electrodes 602 per unit time, the survival status of stem cells near the junction of the survival space and the inactivation space can be understood. A resistance recorder electrically connected to electrode 602 records the change in resistance value per unit time and feeds the recorded data back to the control center in real time. The control center judges the feedback data to decide whether to continue raising the piston ring 202. When the frequency of resistance value change in two adjacent cycles recorded by the resistance recorder is basically similar, the control center can decide to continue raising the piston ring 202 to increase the capacity of the deactivation space. Conversely, when the frequency of high resistance values ​​increases in two adjacent cycles recorded by the resistance recorder, it indicates that there are normal stem cells at the junction of the deactivation space and the survival space, and the control center can then determine that the piston ring 202 should stop rising.

[0021] See Figure 1 , 4 And 6, the observation mechanism 4 includes an observation mirror 401 installed above the petri dish 2 inside the incubator 1, and protective balls 402 are distributed in a ring below the observation mirror 401; The outer wall of the observation mirror 401 is coated with an anti-stick coating. The corners of the observation mirror 401 are rounded and chamfered. The lowest point of the observation mirror 401 is a protective ball 402. The observation mirror 401 is connected to the incubator 1 through another set of control mechanisms 5. The observation microscope 401 is a microscope for observing stem cells. The eyepiece of the observation microscope 401 is located above the incubator 1 and is sealed to the incubator 1 through a flexible material. During the stem cell culture, the staff can observe the stem cells in the culture dish 2 through the observation microscope 401 so as to adjust the parameters of the culture medium and the parameters of the stem cell survival environment in a timely manner. The observation microscope 401 is connected to the incubator 1 through the control mechanism 5. The staff sends a control command to the control mechanism 5 connected to the observation microscope 401 through the control center to control the observation microscope 401 to descend until the observation microscope 401 is deeply inserted into the culture medium and the protective ball 402 at the lower end of the observation microscope 401 touches the lower side wall of the culture dish 2. The protective ball 402 is arc-shaped relative to the protruding part of the observation microscope 401. The setting of the protective ball 402 restricts the direct contact between the observation microscope 401 and the culture dish 2 and maintains a certain distance, so that there is a thin layer of culture medium between the observation microscope 401 and the culture dish 2. This avoids that if the gap between the observation microscope 401 and the culture dish 2 is too large, multiple layers of stem cells at different heights will overlap, which would affect the observation effect of the observation microscope 401. The outer wall structure of the observation lens 401, along with the anti-stick coating on its surface and the protective ball 402, ensures that the culture medium on the surface of the observation lens 401 will not stick together after it leaves the culture dish 2. Furthermore, the culture medium will actively move towards the lowest point of the observation lens 401, i.e., the location of the protective ball 402, under the influence of gravity, and then form droplets that fall off.

[0022] See Figure 6 An airflow channel is provided inside the observation mirror 401, and an opening 403 is provided below the protective ball 402. The output end of the airflow channel is the opening 403. The airflow channel and the direction of the output opening 403 are designed so that when the protective ball 402 at the lower end of the observation mirror 401 is about to contact the culture dish 2, an air pump is installed at the input end of the airflow channel. The air pump pumps the air inside the incubator 1 into the opening 403. The small airflow at the opening 403 blows away the solution near the protective ball 402 to ensure that the stem cells will not rupture and die due to the compression of the protective ball 402 during the contact between the protective ball 402 and the culture dish 2. At the same time, during the observation, the alternating magnetic field generator 3 can be briefly activated to drive the solution below the observation mirror 401. By disturbing the culture medium below the observation mirror 401, the cells in the gap are driven. The stem cells at the lower edge of the observation mirror 401 leave under the action of centrifugal force. By reducing the number of stem cells below the observation mirror 401, the overlapping projections of multiple stem cells are avoided.

[0023] See Figures 1-3A slide rail 301 is provided below the alternating magnetic field generator 3. The alternating magnetic field generator 3 is connected to the incubator 1 through the third set of control mechanisms 5. The alternating magnetic field generator 3 is slidably connected to the slide rail 301. The third set of control mechanisms 5 is used to change the relative position of the alternating magnetic field generator 3 and the culture dish 2. The slide rail 301 and the control mechanism 5 are configured such that, driven by the control mechanism 5, the alternating magnetic field generator 3 can slide horizontally along the slide rail 301. During the period when various components in the culture medium need to be fully mixed, the control mechanism 5 and the slide rail 301 change the relative position of the alternating magnetic field generator 3 and the culture dish 2, thereby changing the center point of the vortex in the culture dish 2, so that the center point of the vortex moves closer to one side of the inner wall of the culture dish 2. Since the distance between the center point of the vortex and the inner walls on both sides of the culture dish 2 is different, the pressure on both sides of the vortex is different. The vortex formed by the culture medium is squeezed by the inner wall of the culture dish 2 on the side closer to the center point and accumulates a certain pressure. Then, after the culture medium moves to the opposite side, there is enough space to release the pressure.

[0024] See Figures 1-2 The control mechanism 5 includes an electric control spring 501 and a limiting sleeve 502. The limiting sleeve 502 is disposed inside the electric control spring 501. The electric control spring 501 is electrically connected to the output power supply. The spring will actively contract after being energized. The setting of the limiting sleeve 502 controls the contraction and extension direction of the electric spring 501. The limiting sleeve 502 is composed of two sleeves of different sizes. There are a total of three control mechanisms 5 in this device, which control the piston ring 202, the observation mirror 401 and the alternating magnetic field generator 3 respectively. All of them are connected to the incubator 1 through the electric spring 501 and the limiting sleeve 502.

[0025] Working principle of the invention: The stem cells to be cultured are placed in a culture dish 2, and then the culture dish 2 is placed above the alternating magnetic field generator 3 in the incubator 1. The alternating magnetic field generator 3 can generate an alternating current during stem cell culture. The culture dish 2 contains a culture medium containing a large number of ions to balance the osmotic pressure on both sides of the cell membrane. Under the action of the alternating magnetic field, an alternating current is formed in the culture medium. Under the action of the Lorentz force, the ions move in the solution, thereby enabling the ions to drive the movement of the stem cells inside the culture medium. Through magnetic convection, the various components in the solution are mixed with each other, while ensuring that the stem cells coexist in the culture medium of the same concentration. At the same time, the incubator 1 is equipped with an industry-standard temperature control mechanism and sterilization mechanism to ensure that the incubator 1 is in a sterile state and that the stem cells survive at a suitable temperature. The observation mechanism 4 is set up to facilitate the observation of the stem cells at various growth stages during the stem cell growth period.

[0026] The main function of the partition ring 201 is to divide the internal space of the culture dish 2 into two spaces: a survival space and a deactivation space. The lower ends of the survival space and the deactivation space are connected. Under the action of the alternating magnetic field, the solution in the culture dish 2 will form a vortex. At the same time, some stem cells will die during the culture period. After the stem cells die, the cell membrane is damaged, and the ions inside the cell membrane will slowly leak out. The cell membrane of normal stem cells has a certain insulating effect. Therefore, after the cell membrane is damaged, the liquid inside the cell membrane and the culture medium in the culture dish 2 provide a basis for electrical connection. The slow leakage of ions from the dead stem cells will cause ion clusters to appear on the outside of the dead stem cells. Under the combined action of the alternating magnetic field and alternating current, the culture medium and the dead stem cells will be subjected to the Lorentz force. Since the dead stem cells are located in the culture medium, the swimming speed of the dead stem cells is greater than the swimming speed of the normal stem cells carried by the culture medium. There is a speed difference between the normal stem cells and the dead stem cells. The upper part of the partition ring 201 is a cylinder, and the lower part of the partition ring 201 is a truncated cone with a vertical cross-section of trapezoidal shape. Normal stem cells and dead stem cells move in a circular motion within the partition ring 201. Under the action of centrifugal force, due to the eddy current motion of the culture medium and the annular shape of the lower part of the partition ring 201, the proportion of dead stem cells is higher in the ring near the inner wall of the partition ring 201, while the proportion of normal stem cells is higher on the side near the central axis of the partition ring 201. In addition, the lower diameter of the partition ring 201 is larger than the upper diameter. Under the action of centrifugal force, dead stem cells mainly gather in the lower part of the partition ring 201, that is, at the junction of the living space and the inactivation space. Therefore, under the action of the alternating magnetic field generator 3, the dead stem cells move from the living space to the inactivation space through the gap between the partition ring 201 and the culture dish 2. A monitoring mechanism 6 is installed at the connection between the living space and the inactivation space. The main function of the monitoring mechanism 6 is to monitor the stem cells passing through the gap between the partition ring 201 and the culture dish 2, and to monitor whether the stem cells passing through are in an inactivated state.

[0027] Under the influence of the velocity difference between normal stem cells and dead stem cells, dead stem cells will actively move towards the side where the inactivation space is located. A piston ring 202 is installed within the inactivation space, and the outer wall size of the piston ring 202 matches the structure of the inactivation space. A control mechanism 5 is installed at the upper end of the piston ring 202, which can control the piston ring 202 to rise or fall relative to the culture dish 2. Changes in the relative position of the piston ring 202 cause a synchronous change in the volume of the inactivation space, thereby controlling the number of stem cells entering the inactivation space. A monitoring mechanism 6 is electrically connected to the control mechanism 5 through a control center within the incubator 1. The monitoring mechanism 6 feeds back the detected data to the control center, which then makes a judgment based on the feedback data and sends control information to the control mechanism 5. That is, when the monitoring mechanism 6 detects... Once a high proportion of dead stem cells are present, the control center will raise the piston ring 202 via the control mechanism 5, thereby increasing the volume of the inactivation space to accommodate more dead cells. This continues until the monitoring mechanism 6 detects that the proportion of nearby normal stem cells begins to rise. At this point, the piston ring 202 will stop rising, and the volume of the inactivation space will be fixed. Normal stem cells in the living space will find it difficult to pass through the gap between the partition ring 201 and the culture dish 2 into the inactivation space because the inactivation space is already saturated, there is no longer a pressure difference between the inactivation space and the living space, and the flow rate of normal stem cells is relatively slower than that of dead stem cells. Under the action of centrifugal force, the relatively high-speed moving dead stem cells will exert a pushing force on the normal stem cells, with the direction of the pushing force being the position of the central axis of the partition ring 201.

[0028] The flow channel 601 is located on the inner lower side of the partition ring 201, at the connection between the survival space and the inactivation space. When stem cells move, they will move in a circular motion around the central axis of the partition ring 201. Multiple sets of flow channels 601 are arranged on the inner lower side of the partition ring 201. The horizontal cross-section of the flow channel 601 is arc-shaped, and the outer wall size of the flow channel 601 matches the inner wall size of the partition ring 201. During the movement of stem cells, some stem cells will enter the flow channel 601. Two electrodes 602 are arranged opposite each other inside the flow channel 601. The electrodes 602 are connected to a power source. When stem cells pass between the two electrodes 602... When the resistance between electrodes 602 increases, i.e., the Coulter effect, a resistance recorder is set in the circuit where electrodes 602 are located. When dead stem cells pass between two electrodes 602, the insulation effect of the cell membrane fails due to cell membrane damage. However, during the passage of dead stem cells, the proportion of culture medium between the two electrodes 602 is relatively small. Therefore, the time between dead stem cells and electrodes 602 is greater than the resistance of the culture medium and much less than the resistance of normal stem cells. By detecting the stem cells passing through the gap between electrodes 602 per unit time, the survival status of stem cells near the junction of the survival space and the inactivation space can be understood. A resistance recorder electrically connected to electrode 602 records the change in resistance value per unit time and feeds the recorded data back to the control center in real time. The control center judges the feedback data to decide whether to continue raising the piston ring 202. When the frequency of resistance value change in two adjacent cycles recorded by the resistance recorder is basically similar, the control center can decide to continue raising the piston ring 202 to increase the capacity of the deactivation space. Conversely, when the frequency of high resistance values ​​increases in two adjacent cycles recorded by the resistance recorder, it indicates that there are normal stem cells at the junction of the deactivation space and the survival space, and the control center can then determine that the piston ring 202 should stop rising.

[0029] The observation microscope 401 is a microscope for observing stem cells. The eyepiece of the observation microscope 401 is located above the incubator 1 and is sealed to the incubator 1 through a flexible material. During the stem cell culture, the staff can observe the stem cells in the culture dish 2 through the observation microscope 401 so as to adjust the parameters of the culture medium and the parameters of the stem cell survival environment in a timely manner. The observation microscope 401 is connected to the incubator 1 through the control mechanism 5. The staff sends a control command to the control mechanism 5 connected to the observation microscope 401 through the control center to control the observation microscope 401 to descend until the observation microscope 401 is deeply inserted into the culture medium and the protective ball 402 at the lower end of the observation microscope 401 touches the lower side wall of the culture dish 2. The protective ball 402 is arc-shaped relative to the protruding part of the observation microscope 401. The setting of the protective ball 402 restricts the direct contact between the observation microscope 401 and the culture dish 2 and maintains a certain distance, so that there is a thin layer of culture medium between the observation microscope 401 and the culture dish 2. This avoids that if the gap between the observation microscope 401 and the culture dish 2 is too large, multiple layers of stem cells at different heights will overlap, which would affect the observation effect of the observation microscope 401. The outer wall structure of the observation lens 401, along with the anti-stick coating on its surface and the protective ball 402, ensures that the culture medium on the surface of the observation lens 401 will not stick together after it leaves the culture dish 2. Furthermore, the culture medium will actively move towards the lowest point of the observation lens 401, i.e., the location of the protective ball 402, under the influence of gravity, and then form droplets that fall off.

[0030] The airflow channel and the direction of the output opening 403 are designed so that when the protective ball 402 at the lower end of the observation mirror 401 is about to contact the culture dish 2, an air pump is installed at the input end of the airflow channel. The air pump pumps the air inside the incubator 1 into the opening 403. The small airflow at the opening 403 blows away the solution near the protective ball 402 to ensure that the stem cells will not rupture and die due to the compression of the protective ball 402 during the contact between the protective ball 402 and the culture dish 2. At the same time, during the observation, the alternating magnetic field generator 3 can be briefly activated to drive the solution below the observation mirror 401. By disturbing the culture medium below the observation mirror 401, the cells in the gap are driven. The stem cells at the lower edge of the observation mirror 401 leave under the action of centrifugal force. By reducing the number of stem cells below the observation mirror 401, the overlapping projections of multiple stem cells are avoided.

[0031] The slide rail 301 and the control mechanism 5 are configured such that, driven by the control mechanism 5, the alternating magnetic field generator 3 can slide horizontally along the slide rail 301. During the period when various components in the culture medium need to be fully mixed, the control mechanism 5 and the slide rail 301 change the relative position of the alternating magnetic field generator 3 and the culture dish 2, thereby changing the center point of the vortex in the culture dish 2, so that the center point of the vortex moves closer to one side of the inner wall of the culture dish 2. Since the distance between the center point of the vortex and the inner walls on both sides of the culture dish 2 is different, the pressure on both sides of the vortex is different. The vortex formed by the culture medium is squeezed by the inner wall of the culture dish 2 on the side closer to the center point and accumulates a certain pressure. Then, after the culture medium moves to the opposite side, there is enough space to release the pressure.

[0032] The spring will actively contract after being energized. The setting of the limiting sleeve 502 controls the contraction and extension direction of the electric spring 501. The limiting sleeve 502 is composed of two sleeves of different sizes. There are a total of three control mechanisms 5 in this device, which control the piston ring 202, the observation mirror 401 and the alternating magnetic field generator 3 respectively. All of them are connected to the incubator 1 through the electric spring 501 and the limiting sleeve 502.

[0033] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0034] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A stem cell activity detection and culture device for treating diabetes, characterized in that: Includes an incubator (1), inside which is a culture dish (2), and below which is an alternating magnetic field generator (3). Inside the culture dish (2) are placed stem cells and culture medium, and the culture medium is an electrolyte solution. When the alternating magnetic field generator (3) is started, it generates an alternating current in the culture dish (2). An observation mechanism (4) is provided on the upper part of the incubator (1). The observation mechanism (4) is located directly above the culture dish (2). The observation mechanism (4) is used to observe the survival status of stem cells in the culture dish (2).

2. The stem cell activity detection and culture device for treating diabetes according to claim 1, characterized in that: The culture dish (2) is provided with a partition ring (201) inside. The partition ring (201) divides the internal space of the culture dish (2) into two parts. The inner space of the partition ring (201) is the survival space, and the space between the partition ring (201) and the culture dish (2) is the inactivation space. The vertical cross section of the lower end of the partition ring (201) is trapezoidal, and the lower surface of the partition ring (201) does not contact the lower side wall inside the culture dish (2). A monitoring mechanism (6) is provided on the inner side of the partition ring (201), which is used to monitor the survival status of stem cells near the inner side of the partition ring (201).

3. The stem cell activity detection and culture device for treating diabetes according to claim 2, characterized in that: A piston ring (202) is provided between the partition ring (201) and the culture dish (2). The piston ring (202) is located in the deactivation space. The lower surface of the piston ring (202) is in contact with the outer wall of the partition ring (201) and the inner wall of the culture dish (2). The upper outer wall size of the piston ring (202) matches the outer wall size of the partition ring (201) and the inner wall size of the culture dish (2). The upper end of the piston ring (202) is provided with a control mechanism (5). The piston ring (202) is connected to the incubator (1) through the control mechanism (5). The control mechanism (5) is used to control the piston ring (202) to rise and fall relative to the culture dish (2). The control mechanism (5) changes the containment space of the deactivation space through the piston ring (202). The control mechanism (5) is connected to the monitoring mechanism (6).

4. The stem cell activity detection and culture device for treating diabetes according to claim 3, characterized in that: The monitoring mechanism (6) includes a flow channel (601) and an electrode (602). The flow channel (601) is located on the inner side of the isolation ring (201) and below. The flow channel (601) is located at the connection between the survival space and the inactivation space. There are multiple sets of flow channels (601) and they are arranged in a ring within the isolation ring (201). Each set of flow channels (601) has two opposing electrodes (602) inside.

5. A stem cell activity detection and culture device for treating diabetes according to claim 1 or 4, characterized in that: The observation mechanism (4) includes an observation mirror (401) installed above the petri dish (2) inside the incubator (1), and protective balls (402) are distributed in a ring below the observation mirror (401). The outer wall of the observation mirror (401) is coated with an anti-stick coating. The corner of the observation mirror (401) is an arc-shaped chamfer. The lowest point of the observation mirror (401) is a protective ball (402). The observation mirror (401) is connected to the incubator (1) through another set of control mechanisms (5).

6. The stem cell activity detection and culture device for treating diabetes according to claim 5, characterized in that: An airflow channel is provided inside the observation mirror (401), and an opening (403) is provided below the protective ball (402). The output end of the airflow channel is the opening (403).

7. The stem cell activity detection and culture device for treating diabetes according to claim 6, characterized in that: The alternating magnetic field generator (3) is provided with a slide rail (301) below it. The alternating magnetic field generator (3) is connected to the incubator (1) through the third set of control mechanisms (5). The alternating magnetic field generator (3) is slidably connected to the slide rail (301). The third set of control mechanisms (5) is used to change the relative position of the alternating magnetic field generator (3) and the petri dish (2).

8. The stem cell activity detection and culture device for treating diabetes according to claim 7, characterized in that: The control mechanism (5) includes an electric control spring (501) and a limiting sleeve (502). The limiting sleeve (502) is disposed inside the electric control spring (501), and the electric control spring (501) is electrically connected to the output power supply.

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