Use of meiomycodes magnificus strain mp1 in promoting plant growth and enhancing plant stress resistance

Abstract

This invention discloses the application of the *M. mage* strain Mp1 in promoting plant growth and enhancing plant resistance to abiotic stress, belonging to the field of plant biology and strain application technology. The preservation number of strain Mp1 is CGMCC NO.31969. Extensive experimental verification shows that spraying or drenching the roots with the fermentation broth or suspension of *M. mage* strain significantly promotes the growth of *Pyrus pyrifolia* seedlings and enhances their resistance to abiotic stress, overcoming the limitation of existing strains in application and broadening the scope of application of *M. mage* strains. This also contributes to the development of disease-preventing and growth-promoting bacterial agents.

Description

Technical Field

[0001] This invention belongs to the field of plant biology and strain application technology, specifically involving the application of Mp1 strain of MigiMegi yeast in promoting plant growth and enhancing plant resistance to abiotic stress. Background Technology

[0002] Microbial inoculants have broad application prospects in promoting plant growth, increasing crop yield, and improving soil. Currently, a wide variety of beneficial microorganisms for plant growth promotion have been reported, mainly including bacteria, fungi, and actinomycetes, such as Bacillus, Trichoderma, and nitrogen-fixing bacteria, which are widely used in agriculture. However, research on the role of antagonistic yeast strains in promoting plant growth is relatively limited.

[0003] The applicant previously identified a strain of *Megamich* yeast that can delay early leaf drop by inhibiting various fungal diseases in pears (Patent No.: ZL 2024 1 1750780.1). Currently, the role of *Megamich* yeast in promoting growth is mainly discussed in its combination with other microorganisms to promote strawberry growth (Application No.: 201710248030.8), but its growth-promoting effect is primarily attributed to the synergistic effect of multiple microorganisms, rather than the effect of *Megamich* yeast alone. Patents concerning antagonistic yeasts mainly focus on postharvest disease control. However, patent reports specifically targeting *Megamich* yeast for directly promoting plant growth are extremely limited.

[0004] Although *Megrichtomyces maggi* has a relatively deep research foundation in biocontrol and winemaking, its application potential as a plant growth promoter has not yet been fully explored and developed. Existing research indicates that *Megrichtomyces maggi* has the potential to produce plant hormones, but systematic studies on its direct promotion of plant growth are still very scarce. Therefore, developing a *Megrichtomyces maggi* strain with a clear plant growth-promoting effect and elucidating its application methods is of significant scientific importance and practical value for expanding the application areas of antagonistic yeasts. Summary of the Invention

[0005] The technical problem to be solved by this invention is how to provide a strain of Maggi-Meg yeast ( Metschnikowia pulcherrima New uses for Mp1.

[0006] The present invention solves the above-mentioned technical problems through the following technical means:

[0007] This invention proposes the Magemyc yeast strain ( Metschnikowia pulcherrima The application of Mp1 or its fermentation broth or bacterial suspension in promoting plant growth, wherein the Mp1 strain ( Metschnikowia pulcherrima The accession number of Mp1 is CGMCC NO.31969.

[0008] Preferably, the fermentation broth refers to some or all of the substances contained in the culture medium for culturing the strain, regardless of the form of the fermentation broth, including substances such as metabolites or secretions of the strain culture products, and the strain itself may also be contained in the fermentation broth.

[0009] Preferably, the bacterial suspension refers to a suspension prepared by collecting bacterial cells using centrifugal force and dissolving them in water.

[0010] Preferably, the plant includes, but is not limited to, pear trees; the types of pear trees include, but are not limited to, one or more of the following: wild pear, Dangshan crisp pear, Qiubai pear, Ya pear, Hongxiang crisp pear, and Cuiguan pear.

[0011] This invention also proposes the Magemyc yeast strain ( Metschnikowia pulcherrima Application of Mp1 or its fermentation broth and bacterial suspension in enhancing the plant's resistance to abiotic stress.

[0012] Preferably, the abiotic stresses include, but are not limited to, drought stress.

[0013] This invention also proposes the Magemyc yeast strain ( Metschnikowia pulcherrima Application of Mp1 or its fermentation broth or bacterial suspension in the preparation of reagents that promote plant growth and / or enhance plant resistance to drought stress.

[0014] Preferably, the active ingredient of the reagent includes the strain of Saccharomyces cerevisiae (Megamix). Metschnikowia pulcherrima Mp1, or one or more of its fermentation broth or bacterial suspension.

[0015] Preferably, the reagent is a strain of Saccharomyces cerevisiae (Megamiprid). Metschnikowia pulcherrima Mp1, its fermentation broth or bacterial suspension is the only effective ingredient.

[0016] Preferably, the active ingredient is a bacterial suspension of the bacterial strain, and the OD of the bacterial suspension is... 600 = 1.0 ~ 2.0.

[0017] Preferably, the fermentation broth content is 3-8% (v / v); more preferably 5% (v / v).

[0018] This invention also proposes a method for promoting plant growth and / or enhancing plant drought stress resistance, comprising the following steps: spraying the plant foliage with a strain of *Megamiprid* (Megamiprid). Metschnikowia pulcherrima Mp1 fermentation broth, bacterial suspension, or bacterial suspension can be applied to the roots.

[0019] Preferably, the treatment is performed once every 7 to 10 days, for a total of 3 times.

[0020] The beneficial effects of this invention are as follows: 1. This invention proposes a strain of *Megamiprid* (…). Metschnikowia pulcherrima This study explores new applications of Mp1 in promoting plant growth and enhancing plant resistance to abiotic stress. It addresses the limitations of existing strains in application, broadens the scope of application for Mp1, and contributes to the development of disease-preventing and growth-promoting agents.

[0021] 2. The Magemyc yeast strain proposed in this invention can promote plant growth and enhance plant resistance to drought stress.

[0022] 3. Through extensive experimental verification, the applicant of this invention found that spraying or drenching the roots with the fermentation broth or bacterial suspension of the Maggimeich yeast strain can significantly promote the growth of pear seedlings and enhance their resistance to abiotic stress.

[0023] 4. This invention broadens the application scope of Maggi Mage yeast strains in plant growth promotion.

[0024] Of course, implementing any product or method of the present invention does not necessarily require achieving all of the advantages described above at the same time. Attached Figure Description

[0025] Figure 1 This invention illustrates the role of the *Megamich. mage* strain in promoting plant growth in Example 1, where Figure A shows the growth-promoting phenotypes of the *Megamich. mage* strain fermentation broth and bacterial suspension; Figures B and C show the contents of chlorophyll a and chlorophyll b, respectively.

[0026] Figure 2 This invention illustrates the role of Midge Meggi yeast cells and fermentation broth in promoting plant growth in Example 1 of this invention; Figure A shows the phenotypic growth of the aboveground and underground parts of pear seedlings, Figure B shows the fresh weight of the underground parts after yeast cell treatment, and Figure C shows the fresh weight of the underground parts after yeast fermentation broth treatment.

[0027] Figure 3 This invention provides an example of the effect of the Midge Mage yeast suspension (foliar spray) in alleviating drought stress in plants. Figure A shows the phenotypic diagram of pear seedlings before and after drought stress, Figure B shows the effect of drought stress on the chlorophyll content of plants, Figure C shows the effect of drought stress on the MDA content of plants, and Figure D shows the effect of drought stress on the proline content of plants.

[0028] Figure 4 This invention relates to the role of Midge Meggi yeast fermentation broth in alleviating plant drought stress in Example 3 of this invention; Figure A shows the phenotypic diagram of pear seedlings before and after drought stress, Figure B shows the effect of drought stress on the chlorophyll content of plants, Figure C shows the effect of drought stress on the MDA content of plants, and Figure D shows the effect of drought stress on the proline content of plants.

[0029] Figure 5This invention presents Example 4 of the effect of the Midge Mage yeast suspension (root irrigation) on alleviating drought stress in plants; Figure A shows the phenotype of pear seedlings before and after drought stress, Figure B shows the effect of drought stress on the chlorophyll content of plants, Figure C shows the effect of drought stress on the MDA content of plants, and Figure D shows the effect of drought stress on the proline content of plants. Detailed Implementation

[0030] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Unless otherwise defined, the technical terms used below have the same meaning as understood by those skilled in the art.

[0031] Unless otherwise specified, the test materials and reagents used in the following examples are commercially available or prepared by known methods.

[0032] Unless otherwise specified, all techniques or conditions described in the embodiments can be performed in accordance with the techniques or conditions described in the literature in this field or in the product manual. Unless otherwise specified, the quantitative experiments in the following embodiments are all repeated three times or more, and the results are averaged.

[0033] Example 1: The effect of Maggi Mage yeast suspension in promoting pear growth To investigate the role of Maggimeiji yeast suspension in promoting pear growth, the applicant studied the effect of yeast suspension on the growth of *Pyrus pyrifolia* seedlings. (The text abruptly ends here, likely due to an incomplete sentence or missing information.) o The *Megami* yeast preserved at C400°C was activated in YPDA solid medium. Single clones were then picked and cultured in liquid YPDA medium. Subsequently, the culture was multiplied at a 1:100 ratio. When the OD of the culture reached a certain level... 600 When the OD value is between 1.0 and 2.0, collect the bacterial cells by centrifugation at 4000 rpm / min. Dilute the bacterial cells with sterile water to an OD value of 1.0 to 2.0. 600 = 1.0 ~ 1.5, and then foliar spraying was performed on the pear seedlings. During spraying, ensure even coverage on both sides of the leaves, until droplets fall from the leaf surface. The roots were then watered. Each treatment consisted of 12 pear seedlings. Treatments were repeated every 7 ~ 10 days for a total of 3 treatments. Afterwards, leaf samples were collected to determine the content of chlorophyll a and chlorophyll b. The results showed that, compared with the control, the plants foliar sprayed with yeast suspension had significantly higher chlorophyll a content, significantly lower chlorophyll b content, and significantly increased underground fresh weight (e.g., ...). Figure 1 and 2 ).

[0034] To investigate the role of Mp1 yeast fermentation broth in promoting plant growth, the yeast fermentation broth prepared in Example 1 was diluted to a volume concentration of 5%. The control group was treated with 5% YPDA. The broth was then evenly sprayed onto uniformly grown pear trees approximately 15 days after transplanting, with foliar spraying every 7-10 days for a total of 3 treatments. Plant phenotypes were observed approximately 21 days after treatment, and the chlorophyll a and chlorophyll b contents were measured. The results showed that, compared with the control group, the chlorophyll a and chlorophyll b contents of pear trees treated with Mp1 fermentation broth were significantly increased, and the fresh weight of the underground parts was also significantly increased (e.g., ...). Figure 1 and 2 (As shown).

[0035] Example 2: The effect of Maggi Mage yeast suspension (foliar spray) in alleviating drought stress in pears To investigate the effect of *MegMeiqi* yeast suspension on plant drought stress, *Pyrus pyrifolia* seedlings treated with foliar spraying three times as described in Example 1 were subjected to drought stress, with 12 seedlings per treatment. After the control group developed drought symptoms, plant phenotypes were observed, and leaves were collected to measure chlorophyll, malondialdehyde (MDA), and proline content. The results showed that the chlorophyll content in the leaves of the yeast suspension treatment group was significantly higher than that in the control group, while the MDA and proline contents were significantly lower, with highly significant differences. This indicates that foliar spraying of *MegMeiqi* yeast suspension alleviated the damage caused by drought stress to a certain extent (e.g., drought stress). Figure 3 ).

[0036] Example 3: The role of Maggi Mage yeast fermentation broth in alleviating drought stress in pears The pear seedlings treated three times in Example 2 were also subjected to drought stress, with 12 seedlings per treatment. When the control group showed drought symptoms, leaves were collected, and the chlorophyll, malondialdehyde (MDA), and proline content were measured. The results showed that the chlorophyll content in the leaves of the 5% yeast fermentation broth treatment group was significantly higher than that in the control group, while the MDA and proline content were significantly lower. This indicates that foliar spraying with 5% Maggimei yeast fermentation broth alleviated the damage caused by drought stress to some extent (e.g., Figure 4 ).

[0037] Example 4: The effect of *Saccharomyces cerevisiae* suspension (root irrigation) in alleviating drought stress in pears To investigate the effects of antagonistic yeast cell root irrigation on plant drought stress, the yeast suspension prepared in Example 1 was used to irrigate pear seedlings, while the control group was treated with water. Each plant was irrigated with the same treatment solution, and 12 pear seedlings were treated per treatment. Drought stress was applied 21 days after treatment, and the chlorophyll, malondialdehyde (MDA), and proline content in the leaves of the treated plants was measured. The results showed that, compared with the control, root irrigation significantly increased the chlorophyll content in pear seedling leaves and significantly decreased the MDA and proline content, thus alleviating the damage caused by drought stress (e.g., chlorophyll, malondialdehyde, and proline). Figure 5 ).

[0038] All other parts not described in detail are existing technologies. Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

[0039] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. Maggimycia strain ( Metschnikowia pulcherrima The application of Mp1 or its fermentation broth or bacterial suspension in promoting plant growth is characterized by, The Magemyc strain ( Metschnikowia pulcherrima The accession number of Mp1 is CGMCC NO.31969.

2. The application according to claim 1, characterized in that, The plant includes pear trees; the types of pear trees include one or more of the following: wild pear, Dangshan crisp pear, Qiubai pear, Ya pear, Hongxiang crisp pear, and Cuiguan pear.

3. Maggimycia strain ( Metschnikowia pulcherrima Application of Mp1 or its fermentation broth and bacterial suspension in enhancing the plant's resistance to abiotic stress.

4. The application according to claim 3, characterized in that, The aforementioned adverse stresses include drought stress.

5. Maggimycia strain ( Metschnikowia pulcherrima Application of Mp1 or its fermentation broth or bacterial suspension in the preparation of reagents that promote plant growth and / or enhance plant resistance to drought stress.

6. The application according to claim 5, characterized in that, The reagent is based on the strain of *Megmycium cerevisiae* (Megmycium cerevisiae). Metschnikowia pulcherrima Mp1, its fermentation broth or bacterial suspension is the only effective ingredient.

7. The application according to claim 6, characterized in that, The active ingredient is a bacterial suspension of the bacterial strain; the OD of the bacterial suspension... 600 =1.0 ~ 2.

0.

8. The application according to claim 6, characterized in that, The fermentation broth content is 3-8%.

9. A method for promoting plant growth and / or enhancing plant drought stress resistance, characterized in that, Includes the following steps: Spray the plant leaves with Maggi Mitchell yeast strain ( Metschnikowia pulcherrima Mp1 fermentation broth, bacterial suspension, or bacterial suspension can be applied to the roots.

10. The method according to claim 9, characterized in that, The treatment was performed once every 7 to 10 days, for a total of 3 treatments.

Images