Preparation process and application of a compound peptide for enhancing immunity

Abstract

This invention belongs to the field of health food and discloses a preparation process and application of a compound peptide that enhances immunity. The peptide is composed of the following components: 5-15 parts yak bone peptide, 3-10 parts marine collagen oligopeptides, 2-8 parts soybean protein peptide, 1-5 parts cordyceps peptide, 1-5 parts wolfberry peptide, 0.5-3 parts sea cucumber intestinal ovum peptide, 0.3-2 parts yeast β-glucan, 0.01-0.1 parts selenomethionine, 0.2-1.5 parts shiitake mycelium polysaccharide, 0.1-1 parts elderberry extract, 0.1-1 parts echinacea extract, 0.05-0.5 parts zinc-lactalbumin complex, and 0.03-0.3 parts ferrous lactate. This invention proposes a preparation process and application of a compound peptide that enhances immunity. By using specific component ratios and manufacturing processes, it not only has excellent immune activation capabilities but also comprehensively regulates the body's immune function. It can be used to prepare foods or health products that enhance immunity.

Description

Technical Field

[0001] This invention belongs to the field of health food, and in particular relates to a preparation process and application of a compound peptide that enhances immunity. Background Technology

[0002] With the fast pace of modern life, increased work pressure, irregular work and rest schedules, and aggravated environmental pollution, the human immune system is more susceptible to external factors and its function declines. Low immunity has become a health problem that plagues a wide range of people. It not only increases the risk of infection, but may also lead to sub-health conditions such as fatigue, weakness, and metabolic disorders, seriously affecting the quality of life.

[0003] Peptides are compounds formed by two or more amino acids linked by peptide bonds. They fall between large protein molecules and small amino acid molecules, and are a class of functional nutrients that possess high activity, are easily absorbed, and can effectively activate the human regenerative system. They are indispensable core substances in human physiological activities, deeply involved in the regulation of various cellular functions. Simultaneously, they are also core raw materials for many active substances in the human body, such as enzymes, hormones, antibodies, and neurotransmitters. Compared with native proteins and monomeric amino acids, peptides possess unique physiological activity and health care value, integrating nutritional supply, health conditioning, and adjuvant therapy functions.

[0004] Currently, many immune-boosting foods and health products on the market suffer from limited functionality and mechanisms of action, making it difficult to achieve comprehensive and efficient immune regulation. This results in low bioavailability of these products, and their effects on improving immunity, quickly replenishing nutrients, and relieving fatigue are not significant. Summary of the Invention

[0005] To address the aforementioned technical problems, this invention proposes a process and application for preparing a complex peptide that enhances immunity. Through the synergistic effect of multiple components, it achieves a significant boost in immunity. Furthermore, the optimized preparation process, including low-temperature treatment, purification, and encapsulation, maximizes the preservation of the activity of each component and improves bioavailability.

[0006] To achieve the above objectives, this invention provides a process for preparing and applying a complex peptide to enhance immunity, comprising the following components in parts by weight: 5-15 parts yak bone peptide, 3-10 parts marine collagen oligopeptides, 2-8 parts soybean protein peptide, 1-5 parts cordyceps peptide, 1-5 parts wolfberry peptide, 0.5-3 parts sea cucumber intestinal ovum peptide, 0.3-2 parts yeast β-glucan, 0.01-0.1 parts selenomethionine, 0.2-1.5 parts shiitake mycelium polysaccharide, 0.1-1 part elderberry extract, 0.1-1 part echinacea extract, 0.05-0.5 parts zinc-lactalbumin complex, and 0.03-0.3 parts ferrous lactate.

[0007] Furthermore, it is made from the following components in parts by weight: 10 parts yak bone peptide, 6 parts marine collagen oligopeptides, 5 parts soybean protein peptide, 3 parts cordyceps peptide, 3 parts wolfberry peptide, 1.5 parts sea cucumber intestinal ovum peptide, 1 part yeast β-glucan, 0.05 parts selenomethionine, 0.8 parts shiitake mycelium polysaccharide, 0.5 parts elderberry extract, 0.5 parts echinacea extract, 0.2 parts zinc-lactalbumin complex, and 0.1 parts ferrous lactate.

[0008] This invention also provides a process for preparing a complex peptide that enhances immunity, comprising the following steps: 1) Material pretreatment: Yeast β-glucan, shiitake mycelium polysaccharide, elderberry extract, and echinacea extract were respectively subjected to low-temperature airflow pulverization and set aside. 2) Preparation of crude peptide solution: Yak bone peptide, marine collagen oligopeptide, soybean protein peptide, cordyceps militaris peptide, wolfberry peptide and sea cucumber intestinal ovum peptide are mixed and added to deionized water, centrifuged in a centrifuge to remove the precipitate and obtain crude peptide solution. 3) Purification: The crude peptide solution obtained in step 2) is filtered through an ultrafiltration membrane to obtain a purified peptide solution; 4) Compounding: Dissolve yeast β-glucan and shiitake mycelium polysaccharide in deionized water, cool, and slowly add the purified peptide solution obtained in step 3). Adjust the pH and stir for 55-65 minutes to obtain a compound solution. Dissolve selenomethionine, zinc-lactalbumin complex and ferrous lactate, and slowly add them to the above compound solution while stirring. Dissolve elderberry extract and echinacea extract in ethanol, and slowly add them dropwise to the compound solution while stirring to obtain a compound solution. 5) Low-temperature embedding: Add β-cyclodextrin to the compound solution obtained in step 4), stir, and embed; 6) Drying and shaping: Spray dry the product after encapsulation in step 5), pulverize it, mix it evenly, and obtain the final complex peptide product.

[0009] Further, the pulverized sample described in step 1) is passed through a 90-110 mesh sieve.

[0010] Further, in step 2), the centrifugation speed is 7000~9000 r / min, and the centrifugation time is 15~30 min.

[0011] Furthermore, the ultrafiltration membrane described in step 3) has a molecular weight cutoff of 10 kDa.

[0012] Furthermore, in step 4), the temperature of the deionized water is 80~90℃, and the stirring time is 25~35min; the cooling temperature is 35~40℃; and the pH is 6.5~7.

[0013] Further, in step 4), the ethanol content is 50%, and the amount used is within 5% of the total mass of the composite solution. Stir for 40-50 minutes.

[0014] Furthermore, in step 5), the amount of β-cyclodextrin added is 8-12% of the total mass of the compound solution.

[0015] This invention also provides the application of an immune-enhancing complex peptide in the preparation of immune-enhancing foods or health products.

[0016] The components of the complex peptide described in this invention are as follows: Yak bone peptides: Collagen peptides derived from yak bones. They primarily provide easily absorbed amino acids, especially glycine, proline, and hydroxyproline. Marine collagen oligopeptides: Derived from fish collagen, with small molecular weight and high absorption rate, complementing yak bone peptides in terms of source (sea and land); Soy protein peptides: High-quality small-molecule protein from plant sources, providing a complete range of essential amino acids, easily digested and absorbed, quickly providing the body with nutrition and energy, combating fatigue, and promoting muscle repair and growth; These three components primarily provide abundant and easily absorbed small-molecule amino acids and oligopeptides, serving as an energy source for the proliferation, differentiation, and functional execution of immune cells (such as lymphocytes and macrophages). Furthermore, their complementary amino acid profiles ensure that the immune system has sufficient nutritional substrates for a rapid response under stress. They also provide the material basis for other components with direct immune-activating functions (such as cordyceps peptides and polysaccharides) to exert their effects.

[0017] Cordyceps militaris peptides: Peptide extracts from Cordyceps militaris (Cordyceps flower), rich in active substances such as cordycepin and adenosine. Their function is to regulate the Th1 / Th2 immune balance, promote macrophage activation and the release of cytokines (such as IL-2 and IFN-γ), and enhance cellular immunity. Goji berry peptides: Peptide extracts from goji berries, rich in goji berry-specific amino acids, have strong antioxidant properties, can eliminate excess free radicals generated in immune responses, protect immune cells from oxidative damage, and also promote lymphocyte transformation; Cordyceps militaris peptides actively activate the immune response, while Lycium barbarum peptides protect activated immune cells through antioxidant effects, preventing them from being overloaded and damaged. This combination allows the immune system to be powerfully activated while maintaining homeostasis and avoiding excessive immune responses.

[0018] Sea cucumber intestinal ovopeptide: Contains bioactive substances such as saponins and gangliosides unique to sea cucumber, which have immune-stimulating and anti-fatigue effects. Sea cucumber intestinal ovopeptide can synergistically enhance the phagocytic capacity of macrophages and the killing activity of NK cells by other peptides and polysaccharides. By regulating different signaling pathways (such as MAPK), it can form synergistic or complementary effects with Cordyceps militaris peptides, further enhancing core immune efficacy.

[0019] Yeast β-glucan: mainly activates innate immunity by activating the Dectin-1 receptor on the surface of macrophages and neutrophils.

[0020] Shiitake mycelium polysaccharides: Active polysaccharides in shiitake mushrooms are also biological response modifiers that can stimulate T cells and NK cells and promote the production of cytokines such as interferon.

[0021] Elderberry extract: Rich in anthocyanins and flavonoids, it is a natural antioxidant and antiviral powerhouse. It is especially known for its ability to shorten the duration of colds and flu. Echinacea extract: can stimulate the production of immune cells and increase the activity of white blood cells. It is often used to prevent and relieve colds and upper respiratory tract infections. The combined use of the two can cover a wider range of virus types and infection stages. After the immune system is fully activated by the core peptide and polysaccharide, they provide targeted defense against specific pathogens (especially viruses), making the compound peptide of the present invention not only able to generally enhance immunity, but also have outstanding value in dealing with viral infections.

[0022] Selenomethionine: a form of organic selenium, is a core component of glutathione peroxidase (GPx), which is responsible for scavenging free radicals and protecting immune cell membranes and functional proteins from oxidative damage; Zinc-whey protein complex: It is an essential component of thymosin and superoxide dismutase (SOD), directly involved in DNA synthesis and cell division, and directly affecting the development and function of T cells; Ferrous lactate: A common form of iron supplementation. Iron is a core component of hemoglobin, responsible for oxygen transport, participating in hemoglobin synthesis, ensuring oxygen supply to immune organs, and also serving as a cofactor for many immune-related enzymes; Of these three minerals, selenium is responsible for protecting against oxidative damage, zinc for driving cell proliferation and differentiation, and iron for ensuring energy and oxygen supply. Together, they ensure that activated immune cells can proliferate rapidly and healthily and function effectively. Furthermore, whey protein, as a zinc carrier, further enhances zinc absorption and utilization.

[0023] Compared with the prior art, the present invention has the following advantages and technical effects: (1) In this invention, yak bone peptides and marine collagen oligopeptides provide basic amino acids to promote the proliferation of immune cells; soybean protein peptides and cordyceps militaris peptides synergistically regulate the Th1 and Th2 immune balance and enhance macrophage activity; wolfberry peptides and sea cucumber intestinal oocyte peptides are rich in antioxidants and can reduce free radical damage; yeast β-glucan and shiitake mycelium polysaccharide synergistically activate the Toll-like receptor pathway and enhance the activity of natural killer cells (NK cells); elderberry extract and echinacea extract inhibit viral replication and promote interferon production; selenomethionine, zinc-lactalbumin complex and ferrous lactate act as cofactors to enhance the function of immune cells. The components produce a synergistic effect under specific ratios, jointly enhancing humoral immunity, cellular immunity and innate immunity, thus greatly improving the immune enhancement effect. (2) The present invention employs a series of sophisticated processes such as stepwise compounding, low-temperature encapsulation, and temperature-controlled spray drying, which significantly improves the stability and bioavailability of heat-sensitive components and ensures the preservation of the activity of the final product during processing and storage. (3) The composite peptide provided by the present invention has good solubility, stability and human absorption efficiency, and can be widely used in health foods or functional foods in various dosage forms such as tablets, capsules and powders, and has good industrialization prospects. Detailed Implementation

[0024] The technical solution of the present invention will be further described below through embodiments.

[0025] Unless otherwise defined, the technical or scientific terms used in this invention shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention pertains.

[0026] Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this invention. Experimental methods in the following embodiments that do not specify specific conditions are generally determined according to national standards. Experimental instruments, equipment, and reagents in the following embodiments that do not specify their sources are all commercially available materials.

[0027] Unless otherwise defined or stated, all technical and scientific terms used in this invention have the same meaning as those skilled in the art. Furthermore, any methods and materials similar to or equivalent to those described herein can be applied to the methods of this invention. It should be noted that, unless otherwise specified, the embodiments and features described in this invention can be combined with each other.

[0028] Example 1 A process for preparing and applying a complex peptide to enhance immunity, comprising the following components in parts by weight: 10 parts yak bone peptide, 6 parts marine collagen oligopeptides, 5 parts soybean protein peptide, 3 parts cordyceps peptide, 3 parts wolfberry peptide, 1.5 parts sea cucumber intestinal ovum peptide, 1 part yeast β-glucan, 0.05 parts selenomethionine, 0.8 parts shiitake mycelium polysaccharide, 0.5 parts elderberry extract, 0.5 parts echinacea extract, 0.2 parts zinc-lactalbumin complex, and 0.1 parts ferrous lactate.

[0029] The preparation process of an immune-enhancing complex peptide includes the following steps: 1) Material pretreatment: Yeast β-glucan, shiitake mycelium polysaccharide, elderberry extract and echinacea extract were respectively subjected to low-temperature airflow pulverization and passed through a 100-mesh sieve for later use; 2) Preparation of crude peptide solution: Yak bone peptide, marine collagen oligopeptide, soybean protein peptide, cordyceps militaris peptide, wolfberry peptide and sea cucumber intestinal ovum peptide are mixed and added to deionized water, and centrifuged at 8000 r / min for 20 min to remove the precipitate and obtain crude peptide solution. 3) Purification: The crude peptide solution obtained in step 2) is passed through a 10 kDa ultrafiltration membrane to remove macromolecular impurities, resulting in a purified peptide solution; 4) Compounding: Add yeast β-glucan and shiitake mycelium polysaccharide to 85℃ deionized water, stir for 30 min to dissolve, cool to 37℃, and slowly add the purified peptide solution obtained in step 3). Adjust the pH to 6.8, stir for 60 min to form a stable "peptide-polysaccharide" composite solution (the polysaccharide binds to the active sites of peptides through hydrogen bonds to avoid polymerization). Dissolve selenomethionine, zinc-whey protein complex and ferrous lactate in a small amount of warm water, and slowly add them to the above compound solution. Stir for 30 minutes to prevent trace elements from directly chelating with peptides and thus reducing activity. Elderberry extract and echinacea extract were dissolved in 50% ethanol and slowly added dropwise to the compound solution at 27°C. The mixture was stirred for 45 minutes. The amount of ethanol was controlled to be less than 5% of the total mass of the compound solution to obtain the compound solution. 5) Low-temperature encapsulation: Add β-cyclodextrin to the compound solution at a rate of 10% of the total mass of the compound solution, and stir for 30 minutes to encapsulate (to protect heat-sensitive components such as cordyceps militaris peptide and elderberry extract). 6) Drying and shaping: Low-temperature spray drying at an inlet air temperature of 140℃ and an outlet air temperature of 60℃ to obtain a micro-powdered composite peptide semi-finished product; the semi-finished product is then pulverized through a 200-mesh sieve and mixed evenly to obtain the final composite peptide product.

[0030] Example 2 A process for preparing and applying a complex peptide to enhance immunity, comprising the following components in parts by weight: 5 parts yak bone peptide, 3 parts marine collagen oligopeptides, 2 parts soybean protein peptide, 1 part cordyceps peptide, 1 part wolfberry peptide, 0.5 parts sea cucumber intestinal ovum peptide, 0.3 parts yeast β-glucan, 0.01 parts selenomethionine, 0.2 parts shiitake mycelium polysaccharide, 0.1 parts elderberry extract, 0.1 parts echinacea extract, 0.05 parts zinc-lactalbumin complex, and 0.03 parts ferrous lactate.

[0031] The preparation process of the immune-enhancing complex peptide is the same as that in Example 1.

[0032] Example 3 A process for preparing and applying a complex peptide to enhance immunity, comprising the following components in parts by weight: 15 parts yak bone peptide, 10 parts marine collagen oligopeptides, 8 parts soybean protein peptide, 5 parts cordyceps peptide, 5 parts wolfberry peptide, 3 parts sea cucumber intestinal ovum peptide, 2 parts yeast β-glucan, 0.1 parts selenomethionine, 1.5 parts shiitake mycelium polysaccharide, 1 part elderberry extract, 1 part echinacea extract, 0.5 parts zinc-lactrate complex, and 0.3 parts ferrous lactate.

[0033] The preparation process of the immune-enhancing complex peptide is the same as that in Example 1.

[0034] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that: 0 parts of elderberry extract.

[0035] Comparative Example 2 The difference between Comparative Example 1 and Example 1 is that: 0 parts of shiitake mycelium polysaccharide.

[0036] Comparative Example 3 The difference between Comparative Example 1 and Example 1 is that: 0 parts of selenomethionine.

[0037] Comparative Example 4 The difference between Comparative Example 1 and Example 1 is that: 0 parts of wolfberry peptide.

[0038] Comparative Example 5 The difference between Comparative Example 1 and Example 1 is that: in the preparation process of a compound peptide that enhances immunity, in step 4), yeast β-glucan, lentinan mycelium polysaccharide selenomethionine, zinc-whey protein complex, ferrous lactate, elderberry extract, and echinacea extract are directly mixed with deionized water and then added to the purified peptide solution obtained in step 3) to obtain the compound solution.

[0039] Comparative Example 6 The difference between Comparative Example 1 and Example 1 is that the low-temperature encapsulation process in step 5) is missing in the preparation process of a complex peptide that enhances immunity.

[0040] Experimental Example 1 In vitro immunoassay: Mouse macrophages RAW264.7 were collected and divided into a blank control group (no sample added), Example 1-3 groups, and Comparative Examples 1-6 groups. The corresponding samples were added to Example 1-3 groups and Comparative Examples 1-6 groups at a concentration of 100 μg / mL. After culturing for 24 hours, cell proliferation rate (MTT assay), phagocytic activity (neutral red phagocytosis assay), and NO release (Griess assay) were measured. The results are shown in Table 1.

[0041] Table 1. Results of in vitro immunohistochemical cell assays in each experimental group of the macrophage model.

[0042] Examples 1-3 significantly promoted macrophage proliferation, phagocytic activity and NO release (p<0.01), while comparative examples 1-6 showed significantly poorer results.

[0043] Among them, the indicators of Comparative Examples 1 to 4 were significantly lower than those of Example 1, indicating that the absence of a single component would destroy the overall synergistic effect, and that the components have a synergistic effect. The indicators of Comparative Example 5, which was directly mixed and compounded, were the lowest, indicating that direct mixing would cause the active ingredients to interact, precipitate or become inactive due to sudden changes in pH and solvent polarity, verifying the necessity of the stepwise compounding process to ensure the activity of the product. The indicators of Comparative Example 6 were lower than those of Example 1, indicating that β-cyclodextrin encapsulation can effectively protect heat-sensitive components and reduce their degradation in subsequent processing. If this step is missing, some active ingredients will become ineffective, resulting in a decrease in the immune enhancement effect.

[0044] It is evident that the composite peptide of the present invention can effectively activate macrophage function. The increased proliferation rate means an increase in the number of immune cells, the enhanced phagocytic activity reflects the improved ability of macrophages to clear pathogens, and the increased NO release reflects the enhanced inflammatory response and bactericidal activity of immune cells, proving that the composite peptide has a significant immune-enhancing effect in vitro.

[0045] Experiment Example 2 Mouse immunity test: One hundred and eighty healthy mice were randomly divided into four groups: a blank control group, a positive control group (orally administered levamisole 50 mg / kg), Example 1 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, Comparative Example 5 group, and Comparative Example 6 group (all administered orally at a dose of 100 mg / kg / day). The mice were administered the drugs for 28 consecutive days. On day 29, serum IgG levels, spleen lymphocyte proliferation rate (ConA-induced), and NK cell activity were measured. The results are shown in Table 2. Table 2. Results of immune function tests in each experimental group of mice in the mouse model.

[0046] Experimental results showed that the Example 1 group significantly enhanced humoral immunity, cellular immunity and NK cell activity in mice (p<0.01), with significantly better effects than the positive control group and the comparative group, proving that the compound peptide of the present invention has a comprehensive immune-enhancing effect.

[0047] Among them, IgG is the core antibody of humoral immunity. The serum IgG level in the group of Example 1 was significantly higher than that in the blank control group and the positive control group, while the ratio of each pair was lower than that in Example 1, indicating that the complex peptide can effectively promote the secretion of antibodies by B lymphocytes and enhance the humoral immune response.

[0048] Lymphocyte proliferation rate is a key indicator reflecting cellular immune function. The OD570 value of the group in Example 1 was significantly higher than that of the positive control group and each control group, indicating that the complex peptide can effectively promote T lymphocyte proliferation and enhance the killing and regulatory functions of cellular immunity.

[0049] NK cells are an important component of innate immunity. In Example 1, the activity of NK cells in the group was much higher than that in the positive control group and other control groups, indicating that the complex peptide can directly activate innate immune cells and enhance the body's ability to quickly clear pathogens.

[0050] In summary, this invention addresses the problems of limited functionality and loss of active ingredients due to process defects in existing immune-enhancing products by providing a multi-component synergistic and optimized immune-enhancing complex peptide and its preparation process. This complex peptide achieves synergistic immune regulation through a specific weight ratio of peptides, polysaccharides, plant extracts, and minerals. Simultaneously, optimized processes such as low-temperature airflow milling, stepwise compounding, β-cyclodextrin encapsulation, and low-temperature spray drying maximize the preservation of the activity of each component, improving the product's bioavailability and stability. Furthermore, the raw materials for this complex peptide are safe, and the preparation process is mature and controllable, allowing for wide application in immune-enhancing foods or health products. It possesses significant practical application value and market potential, providing a novel and efficient immune-regulating product and preparation method for the health food industry.

[0051] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solutions of the present invention, and these modifications or equivalent substitutions cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A complex peptide that enhances immunity, characterized in that, It is made from the following components in parts by weight: 5-15 parts yak bone peptide, 3-10 parts marine collagen oligopeptides, 2-8 parts soybean protein peptide, 1-5 parts cordyceps peptide, 1-5 parts wolfberry peptide, 0.5-3 parts sea cucumber intestinal ovum peptide, 0.3-2 parts yeast β-glucan, 0.01-0.1 parts selenomethionine, 0.2-1.5 parts shiitake mycelium polysaccharide, 0.1-1 part elderberry extract, 0.1-1 part echinacea extract, 0.05-0.5 parts zinc-lactalbumin complex, and 0.03-0.3 parts ferrous lactate.

2. The complex peptide according to claim 1, characterized in that, It is made from the following components in parts by weight: 10 parts yak bone peptide, 6 parts marine collagen oligopeptides, 5 parts soybean protein peptide, 3 parts cordyceps peptide, 3 parts wolfberry peptide, 1.5 parts sea cucumber intestinal ovum peptide, 1 part yeast β-glucan, 0.05 parts selenomethionine, 0.8 parts shiitake mycelium polysaccharide, 0.5 parts elderberry extract, 0.5 parts echinacea extract, 0.2 parts zinc-lactalbumin complex, and 0.1 parts ferrous lactate.

3. The preparation process of the complex peptide according to any one of claims 1 to 2, characterized in that, Includes the following steps: 1) Material pretreatment: Yeast β-glucan, shiitake mycelium polysaccharide, elderberry extract, and echinacea extract were respectively subjected to low-temperature airflow pulverization and set aside. 2) Preparation of crude peptide solution: Yak bone peptide, marine collagen oligopeptide, soybean protein peptide, cordyceps militaris peptide, wolfberry peptide and sea cucumber intestinal ovum peptide are mixed and added to deionized water, centrifuged in a centrifuge to remove the precipitate and obtain crude peptide solution. 3) Purification: The crude peptide solution obtained in step 2) is filtered through an ultrafiltration membrane to obtain a purified peptide solution; 4) Compounding: Dissolve yeast β-glucan and shiitake mycelium polysaccharide in deionized water, cool, and slowly add the purified peptide solution obtained in step 3). Adjust the pH and stir for 55-65 minutes to obtain a compound solution. Dissolve selenomethionine, zinc-lactalbumin complex and ferrous lactate, and slowly add them to the above compound solution while stirring. Dissolve elderberry extract and echinacea extract in ethanol, and slowly add them dropwise to the compound solution while stirring to obtain a compound solution. 5) Low-temperature embedding: Add β-cyclodextrin to the compound solution obtained in step 4), stir, and embed; 6) Drying and shaping: Spray dry the product after encapsulation in step 5), pulverize it, mix it evenly, and obtain the final complex peptide product.

4. The preparation process according to claim 3, characterized in that, Step 1) The pulverized sample is passed through a 90-110 mesh sieve.

5. The preparation process according to claim 3, characterized in that, Step 2) The centrifugation speed is 7000~9000 r / min, and the centrifugation time is 15~30 min.

6. The preparation process according to claim 3, characterized in that, The ultrafiltration membrane described in step 3) has a molecular weight cutoff of 10 kDa.

7. The preparation process according to claim 3, characterized in that, Step 4) The temperature of the deionized water is 80~90℃, and the stirring time is 25~35min; the cooling temperature is 35~40℃; and the pH is 6.5~7.

8. The preparation process according to claim 3, characterized in that, Step 4) The ethanol used is 50%, and the amount used is within 5% of the total mass of the compound solution. Stir for 40-50 minutes.

9. The preparation process according to claim 3, characterized in that, Step 5) The amount of β-cyclodextrin added is 8-12% of the total mass of the compound solution.

10. The use of the complex peptide according to any one of claims 1 to 2 in the preparation of food or health products that enhance immunity.

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