Method for rapidly screening high-efficiency alpha-helix antibacterial peptide and application of high-efficiency alpha-helix antibacterial peptide

By extracting characteristic parameters of α-helical antimicrobial peptides and employing a hierarchical screening strategy, the problem of scarce data on plant-derived antimicrobial peptides has been solved. This enables rapid and efficient screening of high-efficiency α-helical antimicrobial peptides on ordinary computers, reducing screening time and costs, and making it suitable for various databases and library design scenarios.

CN122369672AActive Publication Date: 2026-07-10NINGBO UNIV
6 Cites 0 Cited by

Patent Information

Application Number
CN202610822130.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-06-09
Publication Date
2026-07-10
Estimated Expiration
2046-06-09

AI Technical Summary

Technical Problem

Existing technologies struggle to efficiently screen for highly effective α-helical antimicrobial peptides when data on plant-derived antimicrobial peptides are scarce. Furthermore, traditional methods are computationally intensive, time-consuming, and inefficient, failing to meet the demands for rapid screening and reduced research costs.

Method used

By extracting characteristic parameters such as the hydrophobic cluster scores HCS3_HF and HCS4_HF of α-helical antimicrobial peptides, and combining them with hydrophobicity Hyd, hydrophobic moment HMom, net charge z, hydrophobic surface HoF, and hydrophilic surface HiF, a programmatic AMPs key feature calculation system written in Python was established for screening. A hierarchical screening strategy was adopted, including physicochemical constraints, confirmation of amphiphilicity, and screening based on hydrophobic cluster score thresholds. The screening process was optimized by using multi-centroid distance sorting.

Benefits of technology

It achieves efficient screening of billions of sequence spaces on ordinary computers, shortening the screening time to 1-2 hours. The MIC of all screened antimicrobial peptides is controlled within 32μg/mL, which significantly improves screening efficiency and reduces costs. It is suitable for various database and library design scenarios.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention discloses a method for rapidly screening high-efficiency alpha-helix antibacterial peptides and application thereof, and the method comprises the following steps: extracting hydrophobic cluster scores HCH3HF and / or HCS4HF of peptide sequences to be screened, and screening the peptide sequences according to values of the characteristics. The known alpha helix antibacterial peptide with low MIC is used as a multi-centroid reference, and accurate optimization of candidate peptides is realized through a characteristic value interval. The screening system is constructed based on the structure-function relationship of the synergistic effect of the i + 3 and i + 4 hydrophobic clusters of the hydrophobic surface, the alpha-helix antibacterial peptide with low minimum antibacterial activity can be efficiently locked from a huge sequence space on the premise of not depending on large-scale training data, and the research and development period of the antibacterial peptide is remarkably shortened.
Need to check novelty before this filing date? Find Prior Art