An antiviral disinfectant wet tissue based on synergistic effect of biological catalysis sterilization and physical membrane breaking

Abstract

This invention discloses an antiviral disinfectant wipe based on the synergistic effect of biocatalytic sterilization and physical membrane disruption, belonging to the field of disinfectant wipe technology. The disinfectant wipe contains the following raw materials in parts by weight: 85-95 parts spunlace nonwoven fabric, 0.08-0.15 parts modified antimicrobial peptides, 1.5-3 parts modified chitosan, 820-930 parts deionized water, 22-28 parts propylene glycol, 18-22 parts glycerin, 6-9 parts sodium cocoyl glycinate, 0.8-1.2 parts citric acid, 0.8-1.0 parts phenoxyethanol, and 4-7 parts aloe vera extract. This antiviral disinfectant wipe focuses on the synergistic effect of physical membrane disruption and catalytic sterilization. The raw material ratio is scientific, containing highly effective components such as modified antimicrobial peptides and modified chitosan, resulting in excellent sterilization and antiviral effects. The preparation process is standardized, with strict pretreatment of the nonwoven fabric, uniform solution dispersion, and controllable product liquid content. The addition of aloe vera extract makes it mild and non-irritating, allowing for flexible storage and high practicality.

Description

Technical Field

[0001] This invention relates to the field of disinfectant wipes technology, specifically to an antiviral disinfectant wipe based on the synergistic effect of biocatalytic sterilization and physical membrane disruption. Background Technology

[0002] In the field of public health and safety and daily health protection, disinfectant wipes have become an essential product in homes, medical settings, and offices due to their convenience and immediate cleaning and sterilization capabilities. Especially in recent years, with the frequent outbreaks of various infectious diseases, the risk of virus and bacteria transmission has significantly increased, leading to higher market demands for the antiviral efficacy, broad-spectrum bactericidal properties, and safety of disinfectant wipes.

[0003] Currently available disinfectant wipes primarily rely on chemical disinfectants (such as alcohol and quaternary ammonium compounds) to achieve sterilization. However, these products have significant limitations: alcohol-based wipes are highly irritating, easily damaging skin and mucous membranes, and pose a flammable risk; quaternary ammonium disinfectants have a slow sterilization rate and limited effectiveness in inactivating some enveloped viruses. Furthermore, traditional disinfectant wipes often employ a single sterilization mechanism, making them ill-suited to the synergistic spread of multiple pathogens in complex environments. Long-term use of chemical disinfectants may also lead to increased bacterial resistance, further reducing disinfection efficiency.

[0004] Furthermore, existing disinfectant wipes suffer from insufficient compatibility between their base materials and functional ingredients, with some products exhibiting issues such as uneven disinfectant absorption and uncontrolled liquid content, affecting user experience and the stability of disinfection effectiveness. Additionally, most products lack gentle skin-care properties, and long-term use can easily lead to dryness, sensitivity, and other discomfort, limiting their application in scenarios involving infants, young children, and individuals with sensitive skin.

[0005] At the technical level, while the disinfection concept of synergistic effects of physical membrane disruption and catalytic sterilization has garnered attention, existing related products still suffer from insufficient modification of core functional components and imperfect synergistic mechanisms. For example, unmodified antimicrobial peptides are easily degraded by proteases, resulting in poor stability; ordinary chitosan has limited antibacterial activity and poor dispersibility, making it difficult to fully exert its catalytic sterilization efficacy. Therefore, developing a novel disinfectant wipe that combines highly efficient antiviral and bactericidal capabilities with gentleness, non-irritation, and strong stability, and achieving synergistic effects of physical membrane disruption and catalytic sterilization through optimized raw material formulation and preparation processes, has become an important research direction in the field of disinfection products. This is of great significance for improving public health protection levels and meeting diversified market demands. Summary of the Invention

[0006] To address the shortcomings of existing technologies, this invention provides an antiviral disinfectant wipe based on the synergistic effect of biocatalytic sterilization and physical membrane disruption, which solves the problems of limited sterilization efficacy, strong irritation, poor ingredient stability, and insufficient skin-friendliness of existing disinfectant wipes.

[0007] To achieve the above objectives, the present invention provides the following technical solution: An antiviral disinfectant wipe based on the synergistic effect of biocatalytic sterilization and physical membrane disruption comprises the following raw materials in parts by weight: 85-95 parts spunlace nonwoven fabric, 0.08-0.15 parts modified antimicrobial peptide, 1.5-3 parts modified chitosan, 820-930 parts deionized water, 22-28 parts propylene glycol, 18-22 parts glycerin, 6-9 parts sodium cocoyl glycinate, 0.8-1.2 parts citric acid, 0.8-1.0 parts phenoxyethanol, and 4-7 parts aloe vera extract.

[0008] Furthermore, the aloe extract is prepared from Aloe vera leaves through low-temperature extraction, wherein the content of aloe polysaccharides is ≥25wt.% and the content of flavonoids is ≥3wt.%. Low-temperature extraction can preserve the activity of effective ingredients, and the high content of aloe polysaccharides and flavonoids can enhance skin moisturizing and soothing effects, reduce the irritation of disinfectant ingredients to the skin, and at the same time help improve the synergistic antibacterial effect, making it suitable for use on sensitive skin and infants.

[0009] Furthermore, the modified antimicrobial peptide is prepared using the following specific steps: A1. Take an aqueous solution of antimicrobial peptide with a concentration of 12 mg / mL, add N-hydroxysuccinimide, and activate it for 30-35 min at 26-29℃ and a stirring rate of 350 r / min. Then, add a hexadecylamine ethanol solution with a concentration of 10 mg / mL dropwise to the system at a rate of 1.5 mL / min. After the addition is complete, raise the temperature to 42-44℃ and react for 2.2-2.8 h. Keep the temperature constant and add a galactose derivative solution with a concentration of 8 mg / mL, and continue the reaction for 1.1-1.4 h. Dialyze the solution in deionized water using a dialysis bag with a molecular weight cutoff of 8000 Da for 26-34 h, changing the dialysis solution every 5-7 h. Finally, freeze-dry the solution under vacuum of 10 Pa at -35℃ to obtain the first modified antimicrobial peptide. Through carboxyl activation, grafting of hexadecylamine and galactose derivatives, and dialysis drying, the lipid solubility and targeting of the antimicrobial peptide are improved, the affinity for pathogen cell membranes is enhanced, and the initial stability is improved.

[0010] A2. Take the first modified antimicrobial peptide, disperse it in deionized water, and ultrasonically disperse for 22-28 min to form a uniform and stable dispersion; add polyethylene glycol diglycidyl ether to the dispersion, adjust the pH of the system to 8.2-8.8 with 10% sodium hydroxide solution, and stir the reaction at 52-58℃ and 450 r / min for 3.2-3.8 h; slowly add 12% methacrylic acid solution, and simultaneously add 20% ammonium persulfate solution dropwise at a rate of 1 ml / min, and continue the reaction. The reaction should be carried out for 2.1-2.4 hours. After the reaction is completed, the pH of the system is adjusted to 6.8-7.2 with 10% hydrochloric acid solution. After centrifugation, the precipitate is washed 3-4 times with deionized water and then freeze-dried at -35℃ under vacuum of 10 Pa to obtain the second modified antimicrobial peptide. After cross-linking, grafting modification, pH adjustment, centrifugation and washing, pH-responsive carboxyl groups are introduced to enhance the lipophilicity and membrane-breaking activity of the peptide in the acidic microenvironment of the lesion, while maintaining a mild pH. This significantly enhances the anti-protease degradation ability and optimizes the structural stability.

[0011] A3. Take the second modified antimicrobial peptide, dissolve it in dimethyl sulfoxide, add 3-chloro-2-hydroxypropyltrimethylammonium chloride, and stir the reaction at 50°C under nitrogen protection for 4.5 h; then add low molecular weight chitosan and continue the reaction for 2.5 h; after the reaction is complete, pour the system into anhydrous ethanol to precipitate, filter and collect the precipitate, wash it twice with anhydrous ethanol and once with deionized water, and freeze-dry it under vacuum to obtain a dry powder; then dissolve all the dry powder again in dimethyl sulfoxide, add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N- Hydroxysuccinimide was activated at 25°C for 15 min with its carboxyl group. Under nitrogen protection, a solution of 4-maleimide benzoic acid dissolved in dimethyl sulfoxide was slowly added dropwise to the above system at a rate of 0.3-0.5 mL / min, and the reaction was carried out at 25-30°C for 60 min. After the reaction, the system was poured into anhydrous ethanol for secondary precipitation. The precipitate was collected by centrifugation and washed with deionized water until the conductivity of the supernatant was ≤10 μS / cm. The precipitate was then freeze-dried at -35°C under vacuum of 10 Pa for 12 h to obtain the modified antimicrobial peptide. Quaternization, compounding, and secondary precipitation purification enhanced the positive charge density and physical membrane-breaking efficiency. Terminal maleimide pinning modification transformed physical membrane breaking from "temporary pore poking" to "permanent membrane breaking," making the pores irreversible and completely blocking the possibility of bacterial repair and survival, further improving synergistic bactericidal effects, biocompatibility, and storage stability.

[0012] Furthermore, the ratio of the antimicrobial peptide aqueous solution, N-hydroxysuccinimide, hexadecylamine ethanol solution, and galactose derivative solution in A1 is 30ml:0.11-0.12g:28-32ml:6-7.5ml.

[0013] Furthermore, the ratio of the first modified antimicrobial peptide, deionized water, polyethylene glycol diglycidyl ether, methacrylic acid solution, and ammonium persulfate solution in A2 is 13g:110-140ml:9-11g:12-14ml:3.5-4.5ml; the polyethylene glycol diglycidyl ether has a molecular weight of 450-550.

[0014] Furthermore, in the A3 preparation, the ratio of the second modified antimicrobial peptide, dimethyl sulfoxide, 3-chloro-2-hydroxypropyltrimethylammonium chloride, and low molecular weight chitosan in the dry powder is 10g:90mL:7-9g:4.5-5.5g; the ratio of dimethyl sulfoxide, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, and N-hydroxysuccinimide used in the reaction with the dry powder is 85mL:0.6g:0.4g; and the ratio of 4-maleimide benzoic acid dissolved in dimethyl sulfoxide is 0.5-0.8g:5mL.

[0015] Furthermore, the low molecular weight chitosan has a molecular weight of 30,000-50,000 Da and a degree of deacetylation ≥88%. This molecular weight range and high degree of deacetylation can improve the water solubility, dispersibility, and antibacterial activity of chitosan, enabling it to synergistically work with antimicrobial peptides, while also enhancing the compatibility between components and preventing aggregation that could affect the bactericidal effect.

[0016] Furthermore, the modified chitosan is prepared using the following specific steps: B1. Take chitosan, disperse it in deionized water, add 15% acetic acid solution, stir to dissolve, and then ultrasonically disperse for 20-30 min to obtain a uniform chitosan solution. Add 2,3-epoxypropyltrimethylammonium chloride to the solution, adjust the system temperature to 50-60℃, stir at 400 r / min, and react for 4-5 h. During the reaction, add deionized water every 1 h to maintain the system concentration stability. After the reaction, adjust the pH of the system to 7.0-7.5 with 10% sodium hydroxide solution, centrifuge, wash the precipitate with deionized water until neutral, and vacuum dry at 55-65℃ for 10-14 h to obtain the first modified chitosan. Through dissolution, quaternization modification, pH adjustment, washing and drying, the antibacterial activity, water solubility and dispersibility of chitosan are enhanced, creating conditions for subsequent loading of silver nanoparticles.

[0017] B2. Take the first-modified chitosan, disperse it in anhydrous ethanol, add ammonium bicarbonate, and ultrasonically disperse for 15-25 min; slowly add a 15% (w / w) tetraethyl orthosilicate ethanol solution to the system, and simultaneously add 10% (w / w) ammonia water as a catalyst, and stir the reaction at 35-45℃ for 5-7 h; after the reaction is completed, filter and collect the product, place it in a muffle furnace, raise the temperature to 280-320℃ at a rate of 4℃ / min, keep it at that temperature for 0.5-1 h, and cool it to obtain the second-modified chitosan; through sol-gel tetraethyl orthosilicate coating, ammonium bicarbonate pore formation and high-temperature calcination, a porous structure is formed, which increases the specific surface area and improves the loading capacity and structural stability of nano-silver.

[0018] B3. Take the second modified chitosan, disperse it in deionized water, add gelatin, stir to dissolve, adjust the pH of the system to 5.5-6.5 with 10% acetic acid solution, and stir to react at 40-50℃ for 2-3 hours; add 10% tannic acid solution to the system, continue to react at 30-40℃ for 1-2 hours, then add nano-silver, and ultrasonically disperse for 15-20 minutes; cool to room temperature, adjust the pH to 7.0-7.5 with 10% sodium hydroxide solution, stir for 10 minutes to flocculate, centrifuge to collect the precipitate, add deionized water to stir and disperse, centrifuge and wash once more, collect the precipitate again, vacuum dry at 55-65℃ for 12 hours, and then pulverize through a 100-mesh sieve to obtain modified chitosan. Through gelatin-tannic acid crosslinking, in-situ loading and pH adjustment of nano-silver, washing and pulverizing, uniform dispersion of nano-silver is achieved and aggregation is inhibited, enhancing catalytic bactericidal activity and long-lasting effect, while optimizing compatibility with other components.

[0019] Furthermore, the ratio of chitosan, deionized water for dispersion, acetic acid solution, and 2,3-epoxypropyltrimethylammonium chloride in B1 is 20g:120-150ml:8-12ml:10-15g; during the reaction, 2-3ml of deionized water is added every 1 hour to maintain the stability of the system concentration.

[0020] Furthermore, the ratio of the first modified chitosan, anhydrous ethanol, ammonium bicarbonate, tetraethyl orthosilicate ethanol solution, and ammonia in B2 is 15g: 100-130ml: 8-12g: 25-35ml: 4-6ml.

[0021] Furthermore, the ratio of the amount of the second modified chitosan, deionized water, gelatin, tannic acid solution, and nano silver in B3 is 12g:90-110ml:5-8g:3-5ml:0.10-0.18g.

[0022] Furthermore, the modified chitosan has a porous spherical structure with a particle size distribution between 50-200 nm and a specific surface area ≥35 m². 2 / g, in which the loading of nano-silver in modified chitosan is 0.8-1.5wt.%, and the nano-silver particles have a particle size ≤50nm, are uniformly dispersed and show no obvious agglomeration. The porous spherical structure and high specific surface area can improve the loading and exposure area of ​​nano-silver. The small-particle-size and uniformly dispersed nano-silver can efficiently exert its catalytic bactericidal effect, avoiding the efficacy decay caused by agglomeration, and synergistically achieving broad-spectrum and rapid disinfection with modified antimicrobial peptides.

[0023] A method for preparing antiviral disinfectant wipes based on the synergistic effect of biocatalytic sterilization and physical membrane disruption specifically includes the following steps: S1. Take 85-95 portions of spunlace nonwoven fabric, cut them into 20cm×15cm sizes, put them in a sterile oven, dry heat sterilize them at 125-130℃ for 35-40 minutes, take them out and cool them to room temperature in a sterile environment for later use. S2. Take 0.08-0.15 parts of modified antimicrobial peptide and 1.5-3 parts of modified chitosan, add 10-15 parts of deionized water respectively, and ultrasonically disperse at 320-380W for 16-19 minutes to prepare a uniform dispersion. S3. Add 22-28 parts of propylene glycol and 18-22 parts of glycerol to 800-900 parts of deionized water, and stir at 220-280 r / min until completely dissolved; then add 6-9 parts of sodium cocoyl glycinate, heat to 42-44℃, and continue stirring for 22-28 min to obtain the base solution. S4. Add the prepared peptide dispersion and chitosan dispersion to the base solution at the same time, keep the temperature at 42-44℃, increase the stirring speed to 420-480r / min, and stir for 30min; then add 4-7 parts of aloe vera extract and continue stirring for 22-28min to ensure that the system is uniformly mixed. S5. Turn off the heating device and wait for the system temperature to drop to 26-29℃. Add 0.8-1.2 parts of citric acid and stir for 12-18 minutes to adjust the pH of the system to 5.8-6.2. Then add 0.8-1.0 parts of phenoxyethanol and stir for 16-19 minutes to ensure that the preservative is evenly dispersed. S6. Immerse the pretreated spunlace nonwoven fabric completely in the prepared solution and soak it at room temperature and pressure for 70-80 minutes, stirring once every 15 minutes to ensure that the nonwoven fabric fully absorbs the solution. The amount of absorption should be controlled at 2.2-2.8 times the mass of the nonwoven fabric. S7. Take out the soaked non-woven fabric and put it into a special extrusion device to slowly squeeze out excess solution, so that the liquid content of the non-woven fabric is controlled at 72-78%; neatly stack the squeezed wet wipes, put them into a sterile composite packaging bag, and vacuum pack them using a vacuum packaging machine; store the finished product in a cool, dry and ventilated place at 8-28℃.

[0024] This invention provides an antiviral disinfectant wipe based on the synergistic effect of biocatalytic sterilization and physical membrane disruption, which has the following beneficial effects: 1. This invention innovatively employs a synergistic mechanism of physical membrane disruption and catalytic sterilization. Through the synergistic effect of modified antimicrobial peptides and modified chitosan, the disinfection effect is significantly improved. The modified antimicrobial peptides, after multi-step modification, have enhanced stability and can rapidly disrupt the cell membrane structure of pathogens, achieving physical membrane disruption. The modified chitosan has a porous spherical structure with uniformly dispersed loaded silver nanoparticles, enabling it to exert a highly efficient catalytic sterilization effect. The synergy of the two can broadly inactivate pathogens such as bacteria and viruses, solving the problem of limited efficacy of traditional single sterilization mechanisms and making it suitable for protection needs in complex environments.

[0025] 2. The product formula is scientifically formulated, adding high-purity aloe vera extract, combined with moisturizing ingredients such as glycerin and propylene glycol. The pH of the system is precisely adjusted to 5.8-6.2, matching the acid-base environment of human skin. The modified core ingredient has reduced irritation, and combined with the gentle surfactant sodium cocoyl glycinate, it avoids the damage to the skin and mucous membranes caused by traditional disinfectant wipes. Long-term use is less likely to cause dryness, sensitivity, or other discomfort, making it suitable for various usage scenarios, including infants and people with sensitive skin, combining disinfection and skincare benefits.

[0026] 3. The core functional components are modified through specific processes. The modified antimicrobial peptides, after cross-linking and grafting, exhibit enhanced resistance to degradation and are less susceptible to protease damage. The modified chitosan-loaded silver nanoparticles, with a diameter ≤50nm, are uniformly dispersed without aggregation, and their porous structure endows them with excellent adsorption and sustained-release properties. Simultaneously, the strictly controlled solution adsorption and liquid content ensure stable release of the active ingredients during use, avoiding the fluctuations in disinfection effects caused by the easy deactivation and uneven release of ingredients in traditional products, thus extending the product's shelf life and effectiveness. Detailed Implementation

[0027] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0028] Example 1: Preparation of antiviral disinfectant wipes based on the synergistic effect of biocatalytic sterilization and physical membrane disruption. The specific preparation steps are as follows: S1. Take 85 portions of spunlace nonwoven fabric, cut them into 20cm×15cm sizes, put them in a sterile oven, dry heat sterilize them at 125℃ for 35 minutes, take them out and cool them to room temperature in a sterile environment for later use. S2. Take 0.08 parts of modified antimicrobial peptide and 1.5 parts of modified chitosan, add 10 parts of deionized water respectively, and ultrasonically disperse at 320W for 16 minutes to prepare a uniform dispersion. S3. Add 22 parts of propylene glycol and 18 parts of glycerol to 800 parts of deionized water, and stir at 220 r / min until completely dissolved; then add 6 parts of sodium cocoyl glycinate, heat to 42℃, and continue stirring for 22 min to obtain the base solution. S4. Add the prepared peptide dispersion and chitosan dispersion to the base solution at the same time, keep the temperature at 42℃, increase the stirring speed to 420r / min, and stir for 30min; then add 4 parts of aloe vera extract and continue stirring for 22min to ensure that the system is uniformly mixed. S5. Turn off the heating device and wait for the system temperature to drop to 26℃. Add 0.8 parts of citric acid and stir for 12 minutes to adjust the pH of the system to 5.8. Then add 0.8 parts of phenoxyethanol and stir for 16 minutes to ensure that the preservative is evenly dispersed. S6. Immerse the pretreated spunlace nonwoven fabric completely in the prepared solution and soak it at room temperature and pressure for 70 minutes, stirring once every 15 minutes to ensure that the nonwoven fabric fully absorbs the solution. The amount of absorption is controlled at 2.2 times the mass of the nonwoven fabric. S7. Take out the soaked non-woven fabric and put it into a special extrusion device to slowly squeeze out excess solution, so that the liquid content of the non-woven fabric is controlled at 72%; neatly stack the squeezed wet wipes and put them into a sterile composite packaging bag, and vacuum pack them using a vacuum packaging machine; store the finished product in a cool, dry and ventilated place at 8-28℃.

[0029] Example 2: Preparation of antiviral disinfectant wipes based on the synergistic effect of biocatalytic sterilization and physical membrane disruption. The specific preparation steps are as follows: S1. Take 95 portions of spunlace nonwoven fabric, cut them into 20cm×15cm sizes, put them in a sterile oven, dry heat sterilize them at 130℃ for 40 minutes, take them out and cool them to room temperature in a sterile environment for later use. S2. Take 0.15 parts of modified antimicrobial peptide and 3 parts of modified chitosan, add 15 parts of deionized water respectively, and ultrasonically disperse at 380W for 19 minutes to prepare a uniform dispersion. S3. Add 28 parts of propylene glycol and 22 parts of glycerin to 900 parts of deionized water, and stir at 280 r / min until completely dissolved; then add 9 parts of sodium cocoyl glycinate, heat to 44℃, and continue stirring for 28 min to obtain the base solution. S4. Add the prepared peptide dispersion and chitosan dispersion to the base solution at the same time, keep the temperature at 44℃, increase the stirring speed to 480r / min, and stir for 30min; then add 7 parts of aloe vera extract and continue stirring for 28min to ensure that the system is uniformly mixed. S5. Turn off the heating device and wait for the system temperature to drop to 29°C. Add 1.2 parts of citric acid and stir for 18 minutes to adjust the pH of the system to 6.2. Then add 1.0 part of phenoxyethanol and stir for 19 minutes to ensure that the preservative is evenly dispersed. S6. Immerse the pretreated spunlace nonwoven fabric completely in the solution prepared above and soak it at room temperature and pressure for 80 minutes. Stir it every 15 minutes during the soaking period to ensure that the nonwoven fabric fully absorbs the solution. The amount of absorption is controlled at 2.8 times the mass of the nonwoven fabric. S7. Take out the soaked non-woven fabric and put it into a special extrusion device to slowly squeeze out excess solution, so that the liquid content of the non-woven fabric is controlled at 78%; neatly stack the squeezed wet wipes and put them into a sterile composite packaging bag, and vacuum pack them using a vacuum packaging machine; store the finished product in a cool, dry and ventilated place at 8-28℃.

[0030] Example 3: Preparation of antiviral disinfectant wipes based on the synergistic effect of biocatalytic sterilization and physical membrane disruption. The specific preparation steps are as follows: S1. Take 90 portions of spunlace nonwoven fabric, cut them into 20cm×15cm sizes, put them in a sterile oven, dry heat sterilize them at 127℃ for 37 minutes, take them out and cool them to room temperature in a sterile environment for later use. S2. Take 0.1 parts of modified antimicrobial peptide and 2 parts of modified chitosan, add 12 parts of deionized water respectively, and ultrasonically disperse at 350W for 18 minutes to prepare a uniform dispersion. S3. Add 25 parts of propylene glycol and 20 parts of glycerin to 850 parts of deionized water, and stir at 250 r / min until completely dissolved; then add 7 parts of sodium cocoyl glycinate, heat to 43℃, and continue stirring for 25 min to obtain the base solution. S4. Add the prepared peptide dispersion and chitosan dispersion to the base solution at the same time, keep the temperature at 43℃, increase the stirring speed to 450r / min, and stir for 30min; then add 5 parts of aloe vera extract and continue stirring for 25min to ensure that the system is uniformly mixed. S5. Turn off the heating device and wait for the system temperature to drop to 27°C. Add 1 part citric acid and stir for 15 minutes to adjust the pH of the system to 6. Then add 0.9 parts phenoxyethanol and stir for 18 minutes to ensure that the preservative is evenly dispersed. S6. Immerse the pretreated spunlace nonwoven fabric completely in the solution prepared above and soak it at room temperature and pressure for 75 minutes. Stir it once every 15 minutes during the soaking period to ensure that the nonwoven fabric fully absorbs the solution. The amount of absorption is controlled at 2.5 times the mass of the nonwoven fabric. S7. Take out the soaked non-woven fabric and put it into a special extrusion device to slowly squeeze out excess solution, so that the liquid content of the non-woven fabric is controlled at 75%; neatly stack the squeezed wet wipes and put them into a sterile composite packaging bag, and vacuum pack them using a vacuum packaging machine; store the finished product in a cool, dry and ventilated place at 8-28℃.

[0031] Example 4: Preparation of modified antimicrobial peptides. The specific preparation steps are as follows: A1. Take 30 ml of an antimicrobial peptide aqueous solution with a concentration of 12 mg / mL, add 0.11 g of N-hydroxysuccinimide, and activate at 26 °C and a stirring rate of 350 r / min for 30 min; then add 28 ml of a hexadecylamine ethanol solution with a concentration of 10 mg / mL dropwise to the system at a rate of 1.5 ml / min. After the addition is complete, raise the temperature to 42 °C and react for 2.2 h; keep the temperature constant, add 6 ml of a galactose derivative solution with a concentration of 8 mg / mL, and continue the reaction for 1.1 h; dialyze in deionized water using a dialysis bag with a molecular weight cutoff of 8000 Da for 26 h, changing the dialysate every 5 h, and finally freeze-dry under vacuum of 10 Pa at -35 °C to obtain the first modified antimicrobial peptide; A2. Take 13g of the first modified antimicrobial peptide, disperse it in 110ml of deionized water, and sonicate for 22min to form a uniform and stable dispersion; add 9g of polyethylene glycol diglycidyl ether to the dispersion, adjust the pH of the system to 8.2 with 10% sodium hydroxide solution, and stir at 52℃ and 450r / min for 3.2h; slowly add 12ml of 12% methacrylic acid solution, and simultaneously add 3.5ml of 20% ammonium persulfate solution at a rate of 1ml / min, and react for 2.1h; after the reaction is complete, adjust the pH of the system to 6.8 with 10% hydrochloric acid solution, centrifuge, wash the precipitate three times with deionized water, and freeze-dry under vacuum of 10Pa and -35℃ to obtain the second modified antimicrobial peptide; A3. Take 10g of the second modified antimicrobial peptide, dissolve it in 90mL of dimethyl sulfoxide, add 7g of 3-chloro-2-hydroxypropyltrimethylammonium chloride, and stir the reaction at 50℃ under nitrogen protection for 4.5h; then add 4.5g of low molecular weight chitosan and continue the reaction for 2.5h; after the reaction is completed, pour the system into anhydrous ethanol to precipitate, filter and collect the precipitate, wash it twice with anhydrous ethanol and once with deionized water, and freeze-dry it under vacuum to obtain a dry powder; then dissolve all the dry powder again in 85mL of dimethyl sulfoxide, add 0.6g of 1-(3-dimethylaminopropyl)-3-ethylcarbon Diimide hydrochloride and 0.4 g N-hydroxysuccinimide were activated at 25 °C for 15 min. Under nitrogen protection, 0.5 g 4-maleimide benzoic acid was dissolved in 5 mL of dimethyl sulfoxide solution and slowly added dropwise to the above system at a rate of 0.3 mL / min. The reaction was carried out at 25 °C for 60 min. After the reaction was completed, the system was poured into anhydrous ethanol for secondary precipitation. The precipitate was collected by centrifugation and washed with deionized water until the conductivity of the supernatant was ≤10 μS / cm. The modified antimicrobial peptide was obtained by freeze-drying under vacuum of 10 Pa and -35 °C for 12 h.

[0032] Example 5: Preparation of modified antimicrobial peptides. The specific preparation steps are as follows: A1. Take 30 ml of an antimicrobial peptide aqueous solution with a concentration of 12 mg / mL, add 0.12 g of N-hydroxysuccinimide, and activate at 29 °C and a stirring rate of 350 r / min for 35 min; then add 32 ml of a hexadecylamine ethanol solution with a concentration of 10 mg / mL dropwise to the system at a rate of 1.5 ml / min. After the addition is complete, raise the temperature to 44 °C and react for 2.8 h; keep the temperature constant, add 7.5 ml of a galactose derivative solution with a concentration of 8 mg / mL, and continue the reaction for 1.4 h; dialyze in deionized water using a dialysis bag with a molecular weight cutoff of 8000 Da for 34 h, changing the dialysate every 7 h, and finally freeze-dry under vacuum of 10 Pa at -35 °C to obtain the first modified antimicrobial peptide; A2. Take 13g of the first modified antimicrobial peptide, disperse it in 140ml of deionized water, and sonicate for 28min to form a uniform and stable dispersion; add 11g of polyethylene glycol diglycidyl ether to the dispersion, adjust the pH of the system to 8.8 with 10% sodium hydroxide solution, and stir at 58℃ and 450r / min for 3.8h; slowly add 14ml of 12% methacrylic acid solution, and simultaneously add 4.5ml of 20% ammonium persulfate solution at a rate of 1ml / min, and react for 2.4h; after the reaction is completed, adjust the pH of the system to 7.2 with 10% hydrochloric acid solution, centrifuge, wash the precipitate 4 times with deionized water, and freeze-dry under vacuum of 10Pa and -35℃ to obtain the second modified antimicrobial peptide; A3. Take 10g of the second modified antimicrobial peptide, dissolve it in 90mL of dimethyl sulfoxide, add 9g of 3-chloro-2-hydroxypropyltrimethylammonium chloride, and stir the reaction at 50℃ under nitrogen protection for 4.5h; then add 5.5g of low molecular weight chitosan and continue the reaction for 2.5h; after the reaction is completed, pour the system into anhydrous ethanol to precipitate, filter and collect the precipitate, wash it twice with anhydrous ethanol and once with deionized water, and freeze-dry it under vacuum to obtain a dry powder; then dissolve all the dry powder again in 85mL of dimethyl sulfoxide, add 0.6g of 1-(3-dimethylaminopropyl)-3-ethylcarbon Diimide hydrochloride and 0.4 g N-hydroxysuccinimide were activated at 25 °C for 15 min. Under nitrogen protection, 0.8 g 4-maleimide benzoic acid was dissolved in 5 mL of dimethyl sulfoxide solution and slowly added dropwise to the above system at a rate of 0.5 mL / min. The reaction was carried out at 30 °C for 60 min. After the reaction was completed, the system was poured into anhydrous ethanol for secondary precipitation. The precipitate was collected by centrifugation and washed with deionized water until the conductivity of the supernatant was ≤10 μS / cm. The modified antimicrobial peptide was obtained by freeze-drying under vacuum of 10 Pa and -35 °C for 12 h.

[0033] Example 6: Preparation of modified chitosan. The specific preparation steps are as follows: B1. Take 20g of chitosan, disperse it in 120ml of deionized water, add 8ml of 15% acetic acid solution, stir to dissolve, and then sonicate for 20min to obtain a uniform chitosan solution; add 10g of 2,3-epoxypropyltrimethylammonium chloride to the solution, adjust the system temperature to 50℃, stir at 400r / min, and react for 4h. During the reaction, add 2ml of deionized water every 1h to maintain the system concentration stability. After the reaction, adjust the pH of the system to 7.0 with 10% sodium hydroxide solution, centrifuge, wash the precipitate with deionized water until neutral, and vacuum dry at 55℃ for 10h to obtain the first modified chitosan. B2. Take 15g of the first-modified chitosan, disperse it in 100ml of anhydrous ethanol, add 8g of ammonium bicarbonate, and sonicate for 15min. Slowly add 25ml of 15% tetraethyl orthosilicate ethanol solution to the system, and simultaneously add 4ml of 10% ammonia water as a catalyst. Stir and react at 35℃ for 5h. After the reaction is complete, filter and collect the product, place it in a muffle furnace, raise the temperature to 280℃ at a rate of 4℃ / min, keep it at that temperature for 0.5h, and cool to obtain the second-modified chitosan. B3. Take 12g of the second modified chitosan, disperse it in 90ml of deionized water, add 5g of gelatin, stir to dissolve, adjust the pH of the system to 5.5 with 10% acetic acid solution, and stir to react at 40℃ for 2h; add 3ml of 10% tannic acid solution to the system, continue to react at 30℃ for 1h, then add 0.10g of nano silver, and ultrasonically disperse for 15min; cool to room temperature, adjust the pH to 7.0 with 10% sodium hydroxide solution, stir for 10min to flocculate, centrifuge to separate and collect the precipitate, add deionized water to stir and disperse, centrifuge and wash once more, collect the precipitate again, vacuum dry at 55℃ for 12h, and then pulverize through a 100-mesh sieve to obtain modified chitosan.

[0034] Example 7: Preparation of modified chitosan. The specific preparation steps are as follows: B1. Take 20g of chitosan, disperse it in 150ml of deionized water, add 12ml of 15% acetic acid solution, stir to dissolve, and then sonicate for 30min to obtain a uniform chitosan solution; add 15g of 2,3-epoxypropyltrimethylammonium chloride to the solution, adjust the system temperature to 60℃, stir at 400r / min, and react for 5h. During the reaction, add 3ml of deionized water every 1h to maintain the system concentration. After the reaction, adjust the pH of the system to 7.5 with 10% sodium hydroxide solution, centrifuge, wash the precipitate with deionized water until neutral, and vacuum dry at 65℃ for 14h to obtain the first modified chitosan. B2. Take 15g of the first-modified chitosan, disperse it in 130ml of anhydrous ethanol, add 12g of ammonium bicarbonate, and sonicate for 25min. Slowly add 35ml of 15% tetraethyl orthosilicate ethanol solution to the system, and simultaneously add 6ml of 10% ammonia water as a catalyst. Stir the reaction at 45℃ for 7h. After the reaction is completed, filter and collect the product, place it in a muffle furnace, raise the temperature to 320℃ at a rate of 4℃ / min, keep it at that temperature for 1h, and cool it to obtain the second-modified chitosan. B3. Take 12g of the second modified chitosan, disperse it in 110ml of deionized water, add 8g of gelatin, stir to dissolve, adjust the pH of the system to 6.5 with 10% acetic acid solution, and stir to react at 50℃ for 3h; add 5ml of 10% tannic acid solution to the system, continue to react at 40℃ for 2h, then add 0.18g of nano silver, and ultrasonically disperse for 20min; cool to room temperature, adjust the pH to 7.5 with 10% sodium hydroxide solution, stir for 10min to flocculate, centrifuge to separate and collect the precipitate, add deionized water to stir and disperse, centrifuge and wash once more, collect the precipitate again, vacuum dry at 65℃ for 12h, and then pulverize through a 100-mesh sieve to obtain modified chitosan.

[0035] Comparative Example 1: Antiviral disinfectant wipes based on the synergistic effect of biocatalytic sterilization and physical membrane disruption were prepared. The specific preparation steps are as follows: The remaining steps remain unchanged, except that the modified antimicrobial peptide prepared in Example 4 and used in Example 3 are replaced with unmodified antimicrobial peptides to prepare antiviral disinfectant wipes based on the synergistic effect of biocatalytic sterilization and physical membrane disruption.

[0036] Comparative Example 2: Antiviral disinfectant wipes based on the synergistic effect of biocatalytic sterilization and physical membrane disruption were prepared. The specific preparation steps are as follows: The remaining steps remain unchanged, except that the modified chitosan prepared in Example 7 and used in Example 3 is replaced with unmodified chitosan to prepare antiviral disinfectant wipes based on the synergistic effect of biocatalytic sterilization and physical membrane disruption.

[0037] Comparative Example 3: Antiviral disinfectant wipes based on the synergistic effect of biocatalytic sterilization and physical membrane disruption were prepared. The specific preparation steps are as follows: The remaining steps remain unchanged, except that the modified antimicrobial peptide prepared in Example 4 used in Example 3 is replaced with unmodified antimicrobial peptide, and the modified chitosan prepared in Example 7 is replaced with unmodified chitosan, so as to prepare antiviral disinfectant wipes based on the synergistic effect of biocatalytic sterilization and physical membrane disruption.

[0038] Performance testing

[0039]

[0040] Performance test results show that the antiviral disinfectant wipes of Examples 1-3 have excellent overall performance: against H1N1 influenza virus, the virus killing rate within 30 seconds is 99.93% or higher, with Example 3 reaching the highest at 99.98%; against Staphylococcus aureus, the diameter of the inhibition zone after 18 hours is between 18.7mm and 21.2mm, with Example 3 showing the best performance; in terms of long-term protection, the killing rate of Escherichia coli on the surface of the carrier is 90.6%-96.5% after 24 hours, with Example 3 still ranking first; in rabbit skin patch tests, there is no irritation after 24 hours. In contrast, the performance of Comparative Examples 1-3, which replace the unmodified antimicrobial peptide, the unmodified chitosan, and both respectively, is significantly reduced. The highest antiviral killing rate is only 95.81%, the largest inhibition zone diameter is 15.2mm, and the highest long-term killing rate is 66.8%, and there is mild to moderate skin irritation, which fully demonstrates the advantages of the modified ingredients and synergistic mechanism of this invention.

[0041] The above description is merely an example and illustration of the present invention. Those skilled in the art can make various modifications or additions to the specific embodiments described, or use similar methods to replace them, as long as they do not deviate from the invention or exceed the scope defined in the claims, all of which should fall within the protection scope of the present invention.

Claims

1. An antiviral disinfectant wipe based on the synergistic effect of biocatalytic sterilization and physical membrane disruption, characterized in that: It contains the following ingredients by weight: 85-95 parts spunlace nonwoven fabric, 0.08-0.15 parts modified antimicrobial peptide, 1.5-3 parts modified chitosan, 820-930 parts deionized water, 22-28 parts propylene glycol, 18-22 parts glycerin, 6-9 parts sodium cocoyl glycinate, 0.8-1.2 parts citric acid, 0.8-1.0 parts phenoxyethanol, and 4-7 parts aloe vera extract.

2. The antiviral disinfectant wipes based on the synergistic effect of biocatalytic sterilization and physical membrane disruption according to claim 1, characterized in that: The aloe extract is prepared by low-temperature extraction of Aloe vera leaves, wherein the content of aloe polysaccharides is ≥25wt.% and the content of flavonoids is ≥3wt.%.

3. The antiviral disinfectant wipes based on the synergistic effect of biocatalytic sterilization and physical membrane disruption according to claim 1, characterized in that: The modified antimicrobial peptide is prepared using the following specific steps: A1. Take an aqueous solution of antimicrobial peptide with a concentration of 12 mg / mL, add N-hydroxysuccinimide, and activate it for 30-35 min at 26-29℃ and a stirring rate of 350 r / min. Then, add a solution of hexadecylamine ethanol with a concentration of 10 mg / mL dropwise to the system at a rate of 1.5 mL / min. After the addition is complete, raise the temperature to 42-44℃ and react for 2.2-2.8 h. Keep the temperature constant, add a solution of galactose derivative with a concentration of 8 mg / mL, and continue the reaction for 1.1-1.4 h. Dialyze the solution in deionized water using a dialysis bag with a molecular weight cutoff of 8000 Da for 26-34 h, changing the dialysis solution every 5-7 h. Finally, freeze-dry the solution under vacuum of 10 Pa at -35℃ to obtain the first modified antimicrobial peptide. A2. Take the first modified antimicrobial peptide, disperse it in deionized water, and ultrasonically disperse it for 22-28 min to form a uniform and stable dispersion; add polyethylene glycol diglycidyl ether to the dispersion, adjust the pH of the system to 8.2-8.8 with 10% sodium hydroxide solution, and stir the reaction at 52-58℃ and 450 r / min for 3.2-3.8 h; slowly add 12% methacrylic acid solution, and simultaneously add 20% ammonium persulfate solution dropwise at a rate of 1 ml / min, and react for 2.1-2.4 h; after the reaction is completed, adjust the pH of the system to 6.8-7.2 with 10% hydrochloric acid solution, centrifuge, wash the precipitate 3-4 times with deionized water, and freeze-dry it under vacuum of 10 Pa at -35℃ to obtain the second modified antimicrobial peptide; A3. Take the second modified antimicrobial peptide, dissolve it in dimethyl sulfoxide, add 3-chloro-2-hydroxypropyltrimethylammonium chloride, and stir the reaction at 50°C under nitrogen protection for 4.5 h; then add low molecular weight chitosan and continue the reaction for 2.5 h; after the reaction is complete, pour the system into anhydrous ethanol to precipitate, filter and collect the precipitate, wash it twice with anhydrous ethanol and once with deionized water, and freeze-dry it under vacuum to obtain a dry powder; then dissolve all the dry powder again in dimethyl sulfoxide, add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N- Hydroxysuccinimide was activated at 25°C for 15 min. Under nitrogen protection, a solution of 4-maleimide benzoic acid dissolved in dimethyl sulfoxide was slowly added dropwise to the above system at a rate of 0.3-0.5 mL / min, and the reaction was carried out at 25-30°C for 60 min. After the reaction was completed, the system was poured into anhydrous ethanol for secondary precipitation. The precipitate was collected by centrifugation and washed with deionized water until the conductivity of the supernatant was ≤10 μS / cm. The modified antimicrobial peptide was obtained by freeze-drying under vacuum of 10 Pa and -35°C for 12 h.

4. The antiviral disinfectant wipes based on the synergistic effect of biocatalytic sterilization and physical membrane disruption according to claim 3, characterized in that: The volume ratio of the antimicrobial peptide aqueous solution, N-hydroxysuccinimide, hexadecylamine ethanol solution, and galactose derivative solution in A1 is 30 ml: 0.11-0.12 g: 28-32 ml: 6-7.5 ml; The ratio of the first modified antimicrobial peptide, deionized water, polyethylene glycol diglycidyl ether, methacrylic acid solution, and ammonium persulfate solution in A2 is 13g:110-140ml:9-11g:12-14ml:3.5-4.5ml; the polyethylene glycol diglycidyl ether has a molecular weight of 450-550. The ratio of the modified antimicrobial peptide, dimethyl sulfoxide, 3-chloro-2-hydroxypropyltrimethylammonium chloride, and low molecular weight chitosan in the dry powder prepared in A3 is 10g:90mL:7-9g:4.5-5.5g; the ratio of dimethyl sulfoxide, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, and N-hydroxysuccinimide used in the reaction with the dry powder is 85mL:0.6g:0.4g; and the ratio of 4-maleimide benzoic acid dissolved in dimethyl sulfoxide is 0.5-0.8g:5mL.

5. The antiviral disinfectant wipes based on the synergistic effect of biocatalytic sterilization and physical membrane disruption according to claim 3, characterized in that: The low molecular weight chitosan has a molecular weight of 30,000-50,000 Da and a degree of deacetylation ≥88%.

6. The antiviral disinfectant wipes based on the synergistic effect of biocatalytic sterilization and physical membrane disruption according to claim 1, characterized in that: The modified chitosan is prepared using the following specific steps: B1. Take chitosan, disperse it in deionized water, add 15% acetic acid solution, stir to dissolve, and then ultrasonically disperse for 20-30 min to obtain a uniform chitosan solution; add 2,3-epoxypropyltrimethylammonium chloride to the solution, adjust the system temperature to 50-60℃, stir at 400 r / min, and react for 4-5 h. During the reaction, add deionized water every 1 h to maintain the system concentration stability. After the reaction, adjust the pH of the system to 7.0-7.5 with 10% sodium hydroxide solution, centrifuge, wash the precipitate with deionized water until neutral, and vacuum dry at 55-65℃ for 10-14 h to obtain the first modified chitosan. B2. Take the first modified chitosan, disperse it in anhydrous ethanol, add ammonium bicarbonate, and ultrasonically disperse for 15-25 min; slowly add 15% by mass of tetraethyl orthosilicate ethanol solution to the system, and simultaneously add 10% by mass of ammonia water as a catalyst, and stir the reaction at 35-45℃ for 5-7 h; after the reaction is completed, filter and collect the product, place it in a muffle furnace, raise the temperature to 280-320℃ at a rate of 4℃ / min, keep it at that temperature for 0.5-1 h, and cool it to obtain the second modified chitosan; B3. Take the second modified chitosan, disperse it in deionized water, add gelatin, stir to dissolve, adjust the pH of the system to 5.5-6.5 with 10% acetic acid solution, and stir to react for 2-3 hours at 40-50℃; add 10% tannic acid solution to the system, continue to react for 1-2 hours at 30-40℃, then add nano-silver, and ultrasonically disperse for 15-20 minutes; cool to room temperature, adjust the pH to 7.0-7.5 with 10% sodium hydroxide solution, stir for 10 minutes to flocculate, centrifuge to collect the precipitate, add deionized water to stir and disperse, centrifuge and wash once more, collect the precipitate again, vacuum dry at 55-65℃ for 12 hours, and then pulverize through a 100-mesh sieve to obtain modified chitosan.

7. The antiviral disinfectant wipes based on the synergistic effect of biocatalytic sterilization and physical membrane disruption according to claim 6, characterized in that: The ratio of chitosan, deionized water for dispersion, acetic acid solution, and 2,3-epoxypropyltrimethylammonium chloride in B1 is 20g: 120-150ml: 8-12ml: 10-15g; during the reaction, 2-3ml of deionized water is added every 1 hour to maintain the stability of the system concentration. The ratio of the first modified chitosan, anhydrous ethanol, ammonium bicarbonate, tetraethyl orthosilicate ethanol solution, and ammonia in B2 is 15g: 100-130ml: 8-12g: 25-35ml: 4-6ml. The ratio of the second modified chitosan, deionized water, gelatin, tannic acid solution, and nano-silver in B3 is 12g:90-110ml:5-8g:3-5ml:0.10-0.18g.

8. The antiviral disinfectant wipes based on the synergistic effect of biocatalytic sterilization and physical membrane disruption according to claim 6, characterized in that: The modified chitosan has a porous spherical structure with a particle size distribution between 50-200 nm and a specific surface area ≥35 m². 2 / g, wherein the loading of nano-silver in modified chitosan is 0.8-1.5wt.%, and the nano-silver particles have a particle size ≤50nm, and are uniformly dispersed without obvious agglomeration.

9. A method for preparing antiviral disinfectant wipes based on the synergistic effect of biocatalytic sterilization and physical membrane disruption, characterized in that: Specifically, it includes the following steps: S1. Take 85-95 portions of spunlace nonwoven fabric, cut them into 20cm×15cm sizes, put them in a sterile oven, dry heat sterilize them at 125-130℃ for 35-40 minutes, take them out and cool them to room temperature in a sterile environment for later use. S2. Take 0.08-0.15 parts of modified antimicrobial peptide and 1.5-3 parts of modified chitosan, add 10-15 parts of deionized water respectively, and ultrasonically disperse at 320-380W for 16-19 minutes to prepare a uniform dispersion. S3. Add 22-28 parts of propylene glycol and 18-22 parts of glycerol to 800-900 parts of deionized water, and stir at 220-280 r / min until completely dissolved; then add 6-9 parts of sodium cocoyl glycinate, heat to 42-44℃, and continue stirring for 22-28 min to obtain the base solution. S4. Add the prepared peptide dispersion and chitosan dispersion to the base solution at the same time, keep the temperature at 42-44℃, increase the stirring speed to 420-480r / min, and stir for 30min; then add 4-7 parts of aloe vera extract and continue stirring for 22-28min to ensure that the system is uniformly mixed. S5. Turn off the heating device and wait for the system temperature to drop to 26-29℃. Add 0.8-1.2 parts of citric acid and stir for 12-18 minutes to adjust the pH of the system to 5.8-6.

2. Then add 0.8-1.0 parts of phenoxyethanol and stir for 16-19 minutes to ensure that the preservative is evenly dispersed. S6. Immerse the pretreated spunlace nonwoven fabric completely in the prepared solution and soak it at room temperature and pressure for 70-80 minutes, stirring once every 15 minutes to ensure that the nonwoven fabric fully absorbs the solution. The amount of absorption should be controlled at 2.2-2.8 times the mass of the nonwoven fabric. S7. Take out the soaked non-woven fabric and put it into a special extrusion device to slowly squeeze out excess solution, so that the liquid content of the non-woven fabric is controlled at 72-78%; neatly stack the squeezed wet wipes, put them into a sterile composite packaging bag, and vacuum pack them using a vacuum packaging machine; store the finished product in a cool, dry and ventilated place at 8-28℃.

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