Milk powder formula for promoting the growth of children and preparation method thereof

Abstract

The application relates to the technical field of dairy processing, and discloses milk powder formula for promoting the growth of children and a preparation method thereof, which is composed of the following components in percentage by weight: 50-75% of base raw materials, wherein the base raw materials are compounded by raw cow milk powder, full-fat milk powder and skimmed milk powder in a weight ratio of (4-6):(1-1.5):(0.8-1.2); 1-3% of bone collagen peptide, 1-2% of enzymatic bone powder; 3-10% of composite bioactive factors, wherein the composite bioactive factors are composed of colostrum basic protein, BMP-2 simulation peptide, galactooligosaccharide-galactooligosaccharide composite prebiotics and composite probiotics in a weight ratio of (2-5):(0.01-0.05):(2-5):(1-2); and the preparation method of the milk powder formula for promoting the growth of children breaks through the limitation of traditional unified formula, and three gradient formulas are designed according to the bone metabolism rate, the nutrient absorption capacity and the growth and development characteristics of children aged 3-6, children aged 7-12 and children aged 13-16, and the core contents of raw cow milk powder, colostrum basic protein, bone collagen peptide, enzymatic bone powder and minerals are dynamically adjusted to realize precise nutrition supply.

Description

Technical Field

[0001] This invention relates to the field of dairy processing technology, specifically to a milk powder formula that promotes children's growth and its preparation method. Background Technology

[0002] The growth of a child's height is essentially a process of continuous bone growth and development. Healthy bone development not only requires an adequate supply of basic nutrients such as calcium, phosphorus, and vitamin D, but also depends on the synergistic effect of bioactive factors on the proliferation of chondrocytes, differentiation of osteoblasts, and regulation of osteoclast activity. Furthermore, it is inseparable from the integrity of the intestinal mucosa and the protection of intestinal absorption function.

[0003] Currently available milk powders or related preparations for promoting bone development have many technical defects: For example, the patent CN116240143B, "A strain of Lactobacillus plantarum for promoting bone development and its microencapsulated preparation, preparation process and application," relies solely on the effect of a single Lactobacillus plantarum to achieve the effect of promoting bone development. Its mechanism of action is singular, lacking the synergistic effect of multiple components, resulting in limited efficacy. Some products use a uniform and fixed nutritional ratio, failing to consider the differences in bone metabolism rate and nutritional needs of children aged 3-6, 7-12, and 13-16, thus failing to achieve precise nutritional supply and having insufficient adaptability. At the same time, traditional microencapsulation processes generally use a single encapsulation material and fixed process parameters, while the stability requirements of different active ingredients such as probiotics and active peptides vary significantly. A single process leads to the inactivation of some active ingredients at high processing temperatures and in storage environments, reducing the actual efficacy of the product. Most products do not add ingredients that take into account both bone development and intestinal health, such as bone collagen peptides and enzymatically hydrolyzed bone powder. The intestinal absorption barrier is weak, and the utilization rate of calcium and active nutrients is low.

[0004] Therefore, there is an urgent need for a children's growth-promoting milk powder formula and its preparation method that has dynamic nutritional adaptability and high retention rate of active ingredients. Through formula innovation, process optimization and adaptability design, it is possible to fully meet the nutritional needs of children's bone development at different growth stages. Summary of the Invention

[0005] (a) Technical problems to be solved To address the shortcomings of existing technologies, this invention provides a milk powder formula and its preparation method that promotes children's growth. It solves the technical problem that the traditional microencapsulation process, which generally uses a single encapsulation material and fixed process parameters, leads to the easy inactivation of active ingredients. At the same time, it also solves the problems of insufficient intestinal care and low calcium and nutrient absorption efficiency in traditional children's growth-promoting milk powders.

[0006] (II) Technical Solution To achieve the above objectives, the present invention provides the following technical solution: a milk powder formula for promoting children's height growth, comprising the following components by weight percentage: The basic ingredients comprise 50-75% of the total weight. These basic ingredients are a blend of raw milk powder, whole milk powder, and skim milk powder in a weight ratio of (4-6):(1-1.5):(0.8-1.2). Collagen peptides 1-3% are small molecule active peptides with a molecular weight ≤1000Da. They can repair the intestinal mucosa, promote calcium binding and absorption, and nourish the intestines while helping bone growth. Enzymatically hydrolyzed bone meal 1-2%, which is natural enzymatically hydrolyzed bovine bone meal, rich in natural calcium, phosphorus, and amino acids, gently protects the intestines and improves the absorption and utilization of minerals; The compound bioactive factor contains 3-10% of the total bioactive factor. This compound bioactive factor consists of colostrum basic protein, BMP-2 mimic peptide, galactooligosaccharide-fructooligosaccharide prebiotics, and compound probiotics in a weight ratio of (2-5):(0.01-0.05):(2-5):(1-2). The compound probiotics are *Lactobacillus plantarum* and *Lactobacillus rhamnosus* in a 1:1 weight ratio, with each containing 10 live bacteria. 10 -10 11 CFU / g; The mineral content is 1-3%, including 0.8-1.2% calcium carbonate, 0.4-0.6% potassium dihydrogen phosphate, 5-10 μg / 100g vitamin D, and 3-5 mg / 100g zinc gluconate, with a calcium to phosphorus weight ratio of (1.8-2.2):1. Functional excipients 8-18%, including lactose 5-8%, coconut oil 2-4%, and glyceryl monostearate 0.3-0.5%.

[0007] Preferably, it includes the following steps: Step 1: Raw material pretreatment: Put the basic raw materials into a high-speed pulverizer and pulverize them to 60-100 mesh at a speed of 7000-9000 r / min. Then, screen them with a vibrating screen to remove impurities. The screen mesh size is 60-100 mesh and the vibration frequency is 45-55 Hz. Simultaneously pulverize the bone collagen peptides and enzymatically hydrolyzed bone powder to 80-100 mesh, and dry them at room temperature for later use; Step 2: Pretreatment of colostrum basic protein: Put the colostrum basic protein raw material into a high-speed pulverizer and pulverize it to 80-100 mesh at a speed of 7000-9000 r / min. After removing impurities by screening with a vibrating screen, add 4-6 times the weight of deionized water and stir to make a suspension. Keep it at room temperature for later use. Step 3: Microencapsulation: Probiotics and composite encapsulation agent are mixed at a volume ratio of 1:(1.8-2.2), homogenized 1-3 times at 30-35 MPa using a high-pressure homogenizer, and spray-dried at an inlet air temperature of 150-170℃, an outlet air temperature of 75-85℃, and an atomization pressure of 0.25-0.35 MPa; BMP-2 mimic peptides and composite encapsulation agent are mixed at a weight ratio of 1:(8-12), and dried in a vacuum freeze dryer at a vacuum degree of 0.008-0.012 MPa, a cold trap temperature of -55 to -45℃, and a drying time of 7-9 hours. Bone collagen peptides and enzymatically hydrolyzed bone powder do not require high-temperature encapsulation and can be directly mixed in powder form to avoid loss of activity. Step 4: Mixing and Homogenizing: Add the basic raw materials, minerals, functional excipients, collagen peptides, and enzymatically hydrolyzed bone powder into a double-helix conical mixer and mix at 25-35 r / min for 18-22 min; add colostrum alkaline protein suspension, compound prebiotic solution, and microcapsule powder, and mix at 12-18 r / min for 13-17 min in a low-speed mixer; add 1.8-2.2 times the weight of deionized water to dissolve, and homogenize 2-4 times in a high-pressure homogenizer at 45-55 MPa; Step 5: Spray drying and packaging: The homogeneous emulsion is dried by a spray dryer with an inlet air temperature of 145-155℃, an outlet air temperature of 72-78℃, and an atomization pressure of 0.2-0.3MPa. The finished product is automatically packaged after being screened by a metal detector, with a sealing temperature of 115-125℃.

[0008] Preferably, in the basic raw materials, raw milk powder accounts for 50-60% of the total weight of the basic raw materials, whole milk powder accounts for 10-15%, and skim milk powder accounts for 8-12%.

[0009] Preferably, the composite bioactive factors comprise, by weight, 2-5% of colostrum basic protein, 0.01-0.05% of BMP-2 mimic peptide, 1-3% of galactooligosaccharides, 1-2% of fructooligosaccharides, 0.5-1% of Lactobacillus plantarum, and 0.5-1% of Lactobacillus rhamnosus.

[0010] Preferably, the viable count of both *Lactobacillus plantarum* and *Lactobacillus rhamnosus* is 10. 10 -10 11 CFU / g, and the two work together to regulate gut microbiota and promote nutrient absorption.

[0011] Preferably, in step three, the probiotic complex encapsulating agent is a sodium alginate-chitosan complex encapsulating agent with a mass ratio of 2.8-3.2:1; the BMP-2 mimic peptide complex encapsulating agent is a β-cyclodextrin-maltodextrin complex encapsulating agent with a mass ratio of 0.8-1.2:4.

[0012] Preferably, in step four, the compound prebiotic solution is prepared by dissolving galactooligosaccharides and fructooligosaccharides with 2.8-3.2 times their weight of deionized water at 40±2℃ and a stirring speed of 75-85 r / min.

[0013] Preferably, in step four, the particle size of the homogenized emulsion is ≤5μm, which improves the solubility and smoothness of the milk powder.

[0014] Preferably, the milk powder is suitable for children aged 3-16 years, and is divided into three formula gradients according to age: 3-6 years, 7-12 years, and 13-16 years, with corresponding adjustments to the content of raw milk powder, colostrum basic protein, and minerals.

[0015] Preferably, the finished product should be stored in an environment with a temperature of ≤25℃ and a humidity of ≤60% to avoid moisture absorption and oxidation that could lead to a decline in quality.

[0016] (III) Beneficial Effects Compared with existing technologies, this invention provides a milk powder formula and its preparation method that promote children's growth, and has the following beneficial effects: 1. The preparation method of this milk powder formula that promotes children's height growth is precisely age-appropriate, meeting the differentiated growth needs and breaking through the limitations of traditional uniform formulas. It is designed with three gradient formulas for children aged 3-6, school-age children aged 7-12, and adolescents aged 13-16, based on their bone metabolism rate, nutrient absorption capacity, and growth and development characteristics. By dynamically adjusting the core contents of raw milk powder, colostrum basic protein, bone collagen peptides, enzymatically hydrolyzed bone meal, and minerals, it achieves precise nutritional supply. The formula for toddlers focuses on basic nutrition and gentle absorption, the formula for school-age children strengthens the calcium-phosphorus ratio and energy supplementation, and the formula for adolescents increases the concentration of active factors and provides nutritional support for bone formation. Each stage of the formula is tailored to the core needs of children's bone development in the corresponding age group, and its adaptability and targeting far exceed those of conventional products.

[0017] 2. This formula and preparation method for promoting children's height growth utilizes a synergistic system of "basic nutrition + bioactive factors + minerals," rather than relying on a single ingredient. Bone collagen peptides repair the intestinal mucosal barrier and combine with calcium ions to form a soluble complex, enhancing intestinal calcium absorption efficiency. Enzymatically hydrolyzed bone powder provides natural calcium, phosphorus, and amino acids, gently protecting the intestines without irritation. Together, they solidify the foundation for intestinal absorption. Among the complex bioactive factors, colostrum basic protein promotes calcium absorption and osteoblast proliferation, while BMP-2 mimic peptide regulates osteoblast differentiation, accelerating bone tissue formation. *Lactobacillus plantarum* and *Lactobacillus rhamnosus* (in a 1:1 ratio) synergistically regulate the intestinal flora, combined with galactooligosaccharide-fructooligosaccharide prebiotics to improve nutrient absorption, providing an intestinal foundation for bone development. The mineral component strictly controls the calcium-to-phosphorus ratio to (1.8-2.2):1, combined with vitamin D to promote calcium absorption and zinc gluconate to assist growth hormone secretion, forming a multi-dimensional closed loop for promoting bone development, with efficacy far exceeding that of products with a single active ingredient. Detailed Implementation

[0018] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0019] A formula for a milk powder that promotes children's growth includes four parts: Basic ingredients: Comprising 50-75% of the total, these are blends of raw milk powder, whole milk powder, and skim milk powder in a weight ratio of (4-6):(1-1.5):(0.8-1.2). Raw milk powder comprises 50-60%, serving as the core ingredient and providing essential nutrients such as high-quality protein and milk fat. Whole milk powder accounts for 10-15%, supplementing with appropriate amounts of fat to enhance the taste and energy supply. Skim milk powder comprises 8-12%, further optimizing the protein-to-fat ratio and preventing excessive fat intake.

[0020] Complex bioactive factors: Comprising 3-10% of total composition, these factors consist of colostrum basic protein, BMP-2 mimic peptide, galactooligosaccharide-fructooligosaccharide prebiotic complex, and complex probiotics. The weight ratio of each component is (2-5):(0.01-0.05):(2-5):(1-2). Specific content is as follows: colostrum basic protein 2-5%, BMP-2 mimic peptide 0.01-0.05%, galactooligosaccharide 1-3%, fructooligosaccharide 1-2%, and Lactobacillus plantarum (live count 10). 10 -10 11 CFU / g) 0.5-1%, Lactobacillus rhamnosus (live count 10)10 -10 11 The CFU / g content is 0.5-1%, and the weight ratio of Lactobacillus plantarum to Lactobacillus rhamnosus is 1:1, ensuring that the two work synergistically to regulate the intestines and promote nutrient absorption.

[0021] Minerals: The overall proportion is 1-3%, including 0.8-1.2% calcium carbonate, 0.4-0.6% potassium dihydrogen phosphate, 5-10 μg / 100g vitamin D, and 3-5 mg / 100g zinc gluconate. The calcium to phosphorus weight ratio is strictly controlled at (1.8-2.2):1, a ratio highly compatible with the calcium-to-phosphorus ratio required for children's bone development, maximizing calcium and phosphorus absorption and utilization. Vitamin D promotes intestinal calcium absorption, preventing bone development problems caused by insufficient calcium deposition. Zinc gluconate participates in the synthesis and secretion of growth hormone, assisting in promoting bone growth.

[0022] Functional excipients: comprising 8-18% of the total, including 5-8% lactose, 2-4% coconut oil, and 0.3-0.5% glyceryl monostearate. Lactose not only provides energy for children but also promotes calcium absorption; coconut oil is rich in medium-chain fatty acids, which are easily digested and absorbed, providing rapid energy replenishment for children's growth and development; glyceryl monostearate, as an emulsifier, can improve the solubility and stability of milk powder and enhance the taste when mixed.

[0023] Hardware device: High-speed pulverizer: Model SF-100, mainly used for pulverizing basic raw materials, colostrum basic protein, bone collagen peptides, and enzymatically hydrolyzed bone powder. Its core operating parameter is set to a speed of 8000 r / min. By setting this parameter, the raw materials can be pulverized into a uniform powder state of 80 mesh, ensuring the uniformity of subsequent mixing processes.

[0024] Vibrating screen: Model ZS-500, used to screen and remove impurities from crushed basic raw materials, colostrum basic protein, bone collagen peptides, and enzymatically hydrolyzed bone powder. Its screen mesh size is set to 80 mesh and the vibration frequency is set to 50Hz. Through high-frequency vibration, impurities and large particles that are not completely crushed in the raw materials are separated, ensuring the purity and particle size consistency of the raw materials after pretreatment.

[0025] Thermostatic stirring tank: Model HJ-100, used for dissolving compound prebiotics. The set temperature is 40±2℃ and the stirring speed is 80r / min. Under these conditions, galactooligosaccharides and fructooligosaccharides can be dissolved quickly and evenly without reducing their activity due to excessive temperature.

[0026] High-pressure homogenizer: Model GJB-100, which has dual functions of probiotic microencapsulation and system homogenization. In the probiotic microencapsulation stage, the pressure is set to 30MPa. The high pressure allows the encapsulating agent and probiotics to fully fuse and form microcapsules. In the subsequent homogenization and refining stage, the pressure is adjusted to 50MPa. The high-pressure shearing action refines the system particles and improves the solubility of milk powder.

[0027] Spray dryer: Model LPG-5, mainly used for drying probiotic microcapsules. Its core operating parameters are set as follows: inlet air temperature 160±5℃, outlet air temperature 80±3℃, and atomization pressure 0.3MPa. This combination of parameters can maintain the number of live probiotics to the maximum extent while rapidly drying the microcapsules.

[0028] Vacuum freeze dryer: Model LGJ-10, used for embedding and drying BMP-2 mimic peptides. During operation, the vacuum degree is maintained at 0.01MPa, the cold trap temperature is set at -50℃, and the total drying time is set at 8h. The low-temperature vacuum environment avoids the inactivation of BMP-2 mimic peptides due to high temperature, thus ensuring their biological activity.

[0029] Double Helix Conical Mixer: Model SHJ-200, responsible for the mixing process of basic components. The speed is set to 30r / min and the mixing time is set to 20min. Its double helix stirring structure can achieve full mixing of basic raw materials with minerals, functional excipients, collagen peptides and enzymatically hydrolyzed bone powder, with a mixing uniformity of over 98%.

[0030] Low-speed mixer: Model DM-50, used for secondary mixing of active ingredients and base powder. The speed is set to 15 r / min and the mixing time is set to 15 min. The low-speed stirring method can avoid the rupture of probiotic microcapsules and active peptide microcapsules caused by high-speed stirring, thus ensuring the integrity of active ingredients.

[0031] Metal detector: Model MJ-300, used for screening impurities in finished products. Its detection sensitivity is set to Fe≥0.3mm and SUS≥0.5mm, which can effectively detect possible metal impurities in finished products and ensure product safety for consumption.

[0032] Automatic packaging machine: Model DXD-100, used for quantitative packaging of finished products. The packaging speed is set to 30 bags / min and the sealing temperature is set to 120℃. High-temperature sealing ensures the airtightness of the packaging, prevents the finished product from absorbing moisture and oxidizing, and extends the shelf life.

[0033] A method for preparing a milk powder formula that promotes children's height growth includes the following steps: Step 1: Raw material pretreatment: Weigh raw milk powder, whole milk powder, and skim milk powder according to the formula ratio, and put them into a high-speed pulverizer (SF-100) for pulverization. After pulverization, transfer the material to a vibrating screen (ZS-500) for screening and impurity removal. Collect the pretreated basic raw materials that pass through an 80-mesh screen. Weigh out bone collagen peptides and enzymatically hydrolyzed bone powder according to the ratio, and pulverize them to 80 mesh simultaneously. After screening, dry them at room temperature for later use. Place the screened raw materials in dry, clean, and sealed containers for later use to avoid moisture absorption and contamination.

[0034] Step 2: Pretreatment of colostrum basic protein: Put the colostrum basic protein raw material into a high-speed pulverizer and pulverize it to 80-100 mesh at a speed of 7000-9000 r / min. After removing impurities by screening with a vibrating screen, add 4-6 times the weight of deionized water and stir to make a suspension. Keep it at room temperature for later use. Dissolving the compound prebiotics: Accurately weigh the galactooligosaccharides and fructooligosaccharides according to the formula ratio, add 3 times the weight of deionized water, put the mixture into a constant temperature stirring tank (HJ-100), start the stirring tank, set the temperature to 40℃ and the stirring speed to 80r / min, and continue stirring for 30min until the galactooligosaccharides and fructooligosaccharides are completely dissolved to obtain a uniform compound prebiotic solution. Keep it at a constant temperature for later use to avoid crystallization after cooling.

[0035] Step 3: Probiotic microencapsulation: Take Lactobacillus plantarum and Lactobacillus rhamnosus bacterial solutions (both with a concentration of 10) according to the formula ratio. 11 The two bacterial solutions were mixed evenly at a weight ratio of 1:1 (CFU / mL). Then, they were mixed with sodium alginate-chitosan composite encapsulation agent (mass ratio 3:1) at a volume ratio of 1:2. After stirring evenly, the mixture was transferred to a high-pressure homogenizer (GJB-100) and homogenized twice at a pressure of 30 MPa to fully integrate the encapsulation agent and probiotics to form a microcapsule suspension. The microcapsule suspension was then sent to a spray dryer (LPG-5) and dried at an inlet air temperature of 160℃, an outlet air temperature of 80℃, and an atomization pressure of 0.3 MPa. After drying, the solid powder was collected, which is the probiotic microcapsule powder, and stored in a low-temperature drying environment for later use. Microencapsulation of the second segment of active peptide: Weigh the BMP-2 mimic peptide according to the formula ratio, and mix it with the β-cyclodextrin-maltodextrin composite encapsulating agent (mass ratio 1:4) at a weight ratio of 1:10. Add an appropriate amount of deionized water and stir to form a uniform suspension. Transfer the suspension to a vacuum freeze dryer (LGJ-10), set the vacuum degree to 0.01MPa and the cold trap temperature to -50℃, start the drying program, and continue drying for 8 hours. After drying, take out the solid product, pulverize it to 80 mesh with a high-speed pulverizer to obtain active peptide microcapsule powder, seal it and store it in a cool and dry place for later use.

[0036] Step 4: Mixing of basic ingredients: Weigh the pretreated basic raw materials, calcium carbonate, potassium dihydrogen phosphate, lactose, coconut oil, glyceryl monostearate, collagen peptides, and enzymatically hydrolyzed bone meal according to the formula ratio. Put all materials into the double helix conical mixer (SHJ-200) at the same time, close the feed inlet, start the mixer, set the speed to 30 r / min, and continue mixing for 20 minutes. After mixing is completed, stop the machine, take out the material, which is the basic mixed powder, for later use. Secondary mixing of active ingredients: Transfer the base mixture powder into a low-speed mixer (DM-50), and then slowly add colostrum alkaline protein suspension, compound prebiotic solution, probiotic microcapsule powder, and active peptide microcapsule powder in sequence. After adding each material, manually stir it evenly before adding the next material. After all materials have been added, start the low-speed mixer, set the speed to 15 r / min, and mix for 15 minutes to ensure that the active ingredients are evenly integrated with the base mixture powder and to avoid microcapsule rupture, thus obtaining a compound mixture powder. Homogenization and refinement: Add 2 times the weight of deionized water to the compound powder and stir at room temperature until completely dissolved to form an emulsion. Transfer the emulsion to a high-pressure homogenizer (GJB-100), adjust the pressure to 50MPa, and homogenize 3 times. Through high-pressure shearing, the particles in the emulsion are refined to a particle size ≤5μm to obtain a homogenized emulsion. This step can significantly improve the solubility and smoothness of milk powder.

[0037] Step 5: Spray drying into powder: The homogenized emulsion is fed into a spray dryer (LPG-5). The equipment parameters are adjusted to inlet air temperature of 150℃, outlet air temperature of 75℃, and atomization pressure of 0.25MPa. The drying program is started. After atomization, the emulsion comes into full contact with the high-temperature hot air, and is quickly dehydrated and dried to form a powdery material, which is the crude milk powder. After collection, it is transferred to a sealed container to cool to room temperature. Finished product screening and packaging: The cooled milk powder crude product is screened through a metal detector (MJ-300) to remove any possible metal impurities. The qualified milk powder is sent to the automatic packaging machine (DXD-100), with the net content of each bag set to 400g and the sealing temperature set to 120℃. The packaging machine is started to package the products in quantitative quantities. After packaging, the finished products are stored in the warehouse in batches. The storage environment should be cool and dry, with a temperature ≤25℃ and humidity ≤60% to avoid moisture absorption and oxidation of the finished products, which would lead to a decline in quality.

[0038] Example 1: Preparation of infant formula suitable for children aged 3-6 years Formula composition (by weight percentage): 55% raw milk powder, 12% whole milk powder, 10% skim milk powder, 1% collagen peptide, 1% enzymatically hydrolyzed bone meal, 3% colostrum basic protein, 0.02% BMP-2 mimic peptide, 1.8% galactooligosaccharides, 1.2% fructooligosaccharides, 0.7% Lactobacillus plantarum, 0.7% Lactobacillus rhamnosus, 0.85% calcium carbonate, 0.45% potassium dihydrogen phosphate, 7μg / 100g vitamin D, 3.5mg / 100g zinc gluconate, 6% lactose, 3% coconut oil, and 0.4% glyceryl monostearate.

[0039] Preparation process parameters: Colostrum basic protein, collagen peptides, and enzymatically hydrolyzed bone powder were pulverized at 8000 r / min and passed through an 80-mesh sieve; the pressure of the high-pressure homogenizer was set to 30 MPa during the probiotic encapsulation stage; the inlet air temperature was 160℃ and the outlet air temperature was 80℃ when using a spray dryer for drying probiotic microcapsules; the drying time was 8 hours in a vacuum freeze dryer; the mixing time was 20 minutes in a double-spiral conical mixer; the mixing time was 15 minutes in a low-speed mixer; the high-pressure homogenizer pressure was 50 MPa during the homogenization and refining stage, and homogenization was performed 3 times; the inlet air temperature was 150℃, the outlet air temperature was 75℃, and the atomization pressure was 0.25 MPa during the final spray drying to form powder.

[0040] Finished product testing results: According to a third-party testing agency, the finished product contains 18.5% protein, 850mg / 100g calcium, and 472mg / 100g phosphorus, with a calcium-to-phosphorus ratio of 1.8:1. The retention rate of collagen peptides and enzymatically hydrolyzed bone meal is over 95%, meeting the bone development needs of children aged 3-6 years. The probiotic live bacteria count is 8.2 × 10⁻⁶. 10 The CFU / g and BMP-2 mimic peptide survival rate were 93%. The experiment was validated using zebrafish skeletal development. Wild-type AB strain zebrafish, 3 days post-fertilization, were fed an aqueous solution of the milk powder of this invention at 250 μg / mL. After treatment at 28℃ for 72 h, alizarin red staining and fluorescence microscopy analysis showed that the fluorescence intensity of the zebrafish vertebrae was 78% higher than that of the normal control group, and the intestinal mucosal integrity was improved by 30%. Furthermore, no zebrafish died or showed obvious abnormal phenotypes during the experiment, indicating that the milk powder has a significant promoting effect on infant skeletal development and good safety.

[0041] Example 2: Preparation of milk powder suitable for school-aged children aged 7-12 Formula composition (by weight percentage): Raw milk powder 58%, whole milk powder 11%, skim milk powder 9%, collagen peptide 2%, enzymatically hydrolyzed bone meal 1.5%, colostrum basic protein 4%, BMP-2 mimic peptide 0.03%, galactooligosaccharides 2.2%, fructooligosaccharides 1.5%, Lactobacillus plantarum 0.8%, Lactobacillus rhamnosus 0.8%, calcium carbonate 1.0%, potassium dihydrogen phosphate 0.5%, vitamin D 8μg / 100g, zinc gluconate 4mg / 100g, lactose 5.5%, coconut oil 3.2%, glyceryl monostearate 0.4%.

[0042] Preparation process parameters: Colostrum basic protein, collagen peptides, and enzymatically hydrolyzed bone powder were pulverized at 8000 r / min and passed through an 80-mesh sieve; the pressure of the high-pressure homogenizer was set to 32 MPa during the probiotic encapsulation stage; the inlet air temperature was 155℃ and the outlet air temperature was 78℃ when using a spray dryer for drying probiotic microcapsules; the drying time was 8.5 h in a vacuum freeze dryer; the mixing time was 22 min in a double-spiral conical mixer; the mixing time was 15 min in a low-speed mixer; the high-pressure homogenizer pressure was 50 MPa during the homogenization and refining stage, and homogenization was performed 3 times; the inlet air temperature was 150℃, the outlet air temperature was 75℃, and the atomization pressure was 0.25 MPa during the final spray drying to form powder.

[0043] Finished product testing results: According to a third-party testing agency, the finished product contains 19.2% protein, 1000mg / 100g calcium, and 500mg / 100g phosphorus, with a calcium-to-phosphorus ratio of 2.0:1. The retention rate of collagen peptides and enzymatically hydrolyzed bone meal is over 94%, and the live probiotic count is 9.5 × 10⁻⁶. 10 The CFU / g and BMP-2 mimic peptide survival rate were 92%. In zebrafish experiments, after administering the milk powder at a concentration of 500 μg / mL in water and treating it at 28℃ for 72 hours, the fluorescence intensity of the zebrafish vertebrae increased by 83% compared to the normal control group. Calcium deposition efficiency was measured using atomic absorption spectrophotometry, and the results showed that the calcium deposition efficiency was 22% higher than the normal control group, and the intestinal nutrient absorption rate was 25% higher, indicating that the milk powder can effectively promote calcium deposition in the bones of school-aged children and accelerate bone development.

[0044] Example 3: Preparation of milk powder suitable for adolescents aged 13-16 Formula composition (by weight percentage): 60% raw milk powder, 10% whole milk powder, 8% skim milk powder, 3% collagen peptide, 2% enzymatically hydrolyzed bone meal, 5% colostrum basic protein, 0.04% BMP-2 mimic peptide, 2.8% galactooligosaccharides, 1.8% fructooligosaccharides, 1.0% Lactobacillus plantarum, 1.0% Lactobacillus rhamnosus, 1.15% calcium carbonate, 0.55% potassium dihydrogen phosphate, 9μg / 100g vitamin D, 4.8mg / 100g zinc gluconate, 5% lactose, 3.5% coconut oil, and 0.4% glyceryl monostearate.

[0045] Preparation process parameters: Colostrum basic protein, collagen peptides, and enzymatically hydrolyzed bone powder were pulverized at 8000 r / min and passed through an 80-mesh sieve; the pressure of the high-pressure homogenizer was set to 35 MPa during the probiotic encapsulation stage; the inlet air temperature was 150℃ and the outlet air temperature was 75℃ when using a spray dryer for drying probiotic microcapsules; the drying time was 9 hours in a vacuum freeze dryer; the mixing time was 25 minutes in a double-spiral conical mixer; the mixing time was 15 minutes in a low-speed mixer; the high-pressure homogenizer pressure was 50 MPa during the homogenization and refining stage, and homogenization was performed 3 times; the final spray drying powder was carried out with an inlet air temperature of 150℃, an outlet air temperature of 75℃, and an atomization pressure of 0.25 MPa.

[0046] Finished product testing results: According to a third-party testing agency, the finished product contains 20.1% protein, 1150mg / 100g calcium, and 523mg / 100g phosphorus, with a calcium-to-phosphorus ratio of 2.2:1; the retention rate of collagen peptides and enzymatically hydrolyzed bone meal is over 93%; and the live probiotic count is 1.1×10⁻⁶. 11 The CFU / g and BMP-2 mimic peptide survival rate were 91%. In zebrafish experiments, after administering the milk powder at a concentration of 1000 μg / mL in water and treating it at 28°C for 72 hours, the fluorescence intensity of the zebrafish vertebrae increased by 88% compared to the normal control group. Osteoblast activity was detected using the MTT assay, and the osteoblast proliferation rate was measured. The results showed that osteoblast activity was increased by 28% compared to the normal control group, and intestinal barrier function was improved by 40%, indicating that the milk powder can significantly enhance osteoblast activity in adolescents, accelerate bone formation, and promote height growth. Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A milk powder formula that promotes children's height growth, characterized in that: It consists of the following components by weight percentage: The basic ingredients comprise 50-75% of the total weight. These basic ingredients are a blend of raw milk powder, whole milk powder, and skim milk powder in a weight ratio of (4-6):(1-1.5):(0.8-1.2). Collagen peptides 1-3% are small molecule active peptides with a molecular weight ≤1000Da. They can repair the intestinal mucosa, promote calcium binding and absorption, and nourish the intestines while helping bone growth. Enzymatically hydrolyzed bone meal 1-2%, which is natural enzymatically hydrolyzed bovine bone meal, rich in natural calcium, phosphorus, and amino acids, gently protects the intestines and improves the absorption and utilization of minerals; The compound bioactive factor contains 3-10% of the total bioactive factor. This compound bioactive factor consists of colostrum basic protein, BMP-2 mimic peptide, galactooligosaccharide-fructooligosaccharide prebiotics, and compound probiotics in a weight ratio of (2-5):(0.01-0.05):(2-5):(1-2). The compound probiotics are *Lactobacillus plantarum* and *Lactobacillus rhamnosus* in a 1:1 weight ratio, with each containing 10 live bacteria. 10 -10 11 CFU / g; The mineral content is 1-3%, including 0.8-1.2% calcium carbonate, 0.4-0.6% potassium dihydrogen phosphate, 5-10 μg / 100g vitamin D, and 3-5 mg / 100g zinc gluconate, with a calcium to phosphorus weight ratio of (1.8-2.2):

1. Functional excipients 8-18%, including lactose 5-8%, coconut oil 2-4%, and glyceryl monostearate 0.3-0.5%.

2. The method for preparing a milk powder formula to promote children's height growth according to claim 1, characterized in that: Includes the following steps: Step 1: Raw material pretreatment: Put the basic raw materials into a high-speed pulverizer and pulverize them to 60-100 mesh at a speed of 7000-9000 r / min. Then, screen them with a vibrating screen to remove impurities. The screen mesh size is 60-100 mesh and the vibration frequency is 45-55 Hz. Simultaneously pulverize the bone collagen peptides and enzymatically hydrolyzed bone powder to 80-100 mesh, and dry them at room temperature for later use; Step 2: Pretreatment of colostrum basic protein: Put the colostrum basic protein raw material into a high-speed pulverizer and pulverize it to 80-100 mesh at a speed of 7000-9000 r / min. After removing impurities by screening with a vibrating screen, add 4-6 times the weight of deionized water and stir to make a suspension. Keep it at room temperature for later use. Step 3: Microencapsulation: Probiotics and composite encapsulation agent are mixed at a volume ratio of 1:(1.8-2.2), homogenized 1-3 times at 30-35 MPa using a high-pressure homogenizer, and spray-dried at an inlet air temperature of 150-170℃, an outlet air temperature of 75-85℃, and an atomization pressure of 0.25-0.35 MPa; BMP-2 mimic peptides and composite encapsulation agent are mixed at a weight ratio of 1:(8-12), and dried in a vacuum freeze dryer at a vacuum degree of 0.008-0.012 MPa, a cold trap temperature of -55 to -45℃, and a drying time of 7-9 hours. Bone collagen peptides and enzymatically hydrolyzed bone powder do not require high-temperature encapsulation and can be directly mixed in powder form to avoid loss of activity. Step 4: Mixing and Homogenizing: Add the basic raw materials, minerals, functional excipients, collagen peptides, and enzymatically hydrolyzed bone powder into a double-helix conical mixer and mix at 25-35 r / min for 18-22 min; add colostrum alkaline protein suspension, compound prebiotic solution, and microcapsule powder, and mix at 12-18 r / min for 13-17 min in a low-speed mixer; add 1.8-2.2 times the weight of deionized water to dissolve, and homogenize 2-4 times in a high-pressure homogenizer at 45-55 MPa; Step 5: Spray drying and packaging: The homogeneous emulsion is dried by a spray dryer with an inlet air temperature of 145-155℃, an outlet air temperature of 72-78℃, and an atomization pressure of 0.2-0.3MPa. The finished product is automatically packaged after being screened by a metal detector, with a sealing temperature of 115-125℃.

3. The milk powder formula for promoting children's height growth according to claim 1, characterized in that: Of the basic raw materials, raw milk powder accounts for 50-60% of the total weight of the basic raw materials, whole milk powder accounts for 10-15%, and skim milk powder accounts for 8-12%.

4. The milk powder formula for promoting children's height growth according to claim 1, characterized in that: The total weight of the compound bioactive factors is as follows: colostrum basic protein 2-5%, BMP-2 mimic peptide 0.01-0.05%, galactooligosaccharides 1-3%, fructooligosaccharides 1-2%, Lactobacillus plantarum 0.5-1%, and Lactobacillus rhamnosus 0.5-1%.

5. The milk powder formula for promoting children's height growth according to claim 1, characterized in that: The viable counts of both *Lactobacillus plantarum* and *Lactobacillus rhamnosus* were 10. 10 -10 11 With CFU / g, both ingredients work synergistically to regulate gut microbiota and promote nutrient absorption. Combined with bone collagen peptides to repair the intestinal mucosa and enzymatically hydrolyzed bone powder to gently protect the intestines, the triple effect strengthens gut health.

6. The method for preparing a milk powder formula to promote children's height growth according to claim 2, characterized in that: In step three, the probiotic complex encapsulating agent is a sodium alginate-chitosan complex encapsulating agent with a mass ratio of 2.8-3.2:1; the BMP-2 mimic peptide complex encapsulating agent is a β-cyclodextrin-maltodextrin complex encapsulating agent with a mass ratio of 0.8-1.2:

4.

7. The method for preparing a milk powder formula to promote children's height growth according to claim 2, characterized in that: In step four, the compound prebiotic solution is prepared by dissolving galactooligosaccharides and fructooligosaccharides with 2.8-3.2 times their weight of deionized water at 40±2℃ and a stirring speed of 75-85 r / min.

8. The method for preparing a milk powder formula to promote children's height growth according to claim 2, characterized in that: In step four, the particle size of the homogenized emulsion is ≤5μm, which improves the solubility and smoothness of the milk powder.

9. The milk powder formula for promoting children's height growth according to claim 1, characterized in that: The milk powder is suitable for children aged 3-16 years and is divided into three formula gradients according to age: 3-6 years, 7-12 years, and 13-16 years. The contents of raw milk powder, colostrum basic protein, collagen peptides, enzymatically hydrolyzed bone meal, and minerals are adjusted accordingly.

10. The method for preparing a milk powder formula to promote children's height growth according to claim 2, characterized in that: The finished product should be stored in an environment with a temperature of ≤25℃ and a humidity of ≤60% to avoid moisture absorption and oxidation that could lead to a decline in quality.