A method for rapid and efficient regeneration of pigskin bean hypocotyls

By using the hypocotyl of *Pseudorasbora spp.* as explants and combining specific culture media and conditions, a highly efficient regeneration system was established, solving the problem of insufficient regeneration systems for *Pseudorasbora spp.* and realizing rapid, efficient regeneration and stable mass production of *Pseudorasbora spp.* seedlings.

CN122397622APending Publication Date: 2026-07-17TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
Filing Date
2026-06-02
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

In the current technology, an in vitro regeneration system for pig manure beans has not yet been established, which limits its gene editing, genetic transformation and molecular breeding research, and lacks an efficient and stable regeneration system.

Method used

Using the hypocotyl of *Pseudorasbora spp.* as explants, and through specific culture medium combinations and culture conditions, including germination, somatic embryo induction, subculture, and rooting culture steps, an efficient regeneration system was established.

Benefits of technology

It achieves rapid and efficient regeneration of *Phyllostachys edulis* seedlings, improves regeneration rate and propagation efficiency, reduces browning rate and yellowing of plants, and enables stable mass production of *Phyllostachys edulis* seedlings.

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Abstract

本发明属于猪屎豆栽培领域,具体涉及一种利用猪屎豆下胚轴快速高效再生的方法。本发明以猪屎豆下胚轴为外植体,以外植体诱导产生体细胞胚获得再生植株,建立了一种再生率显著高于子叶的高效离体再生体系,具有效率高,诱导迅速的优点,体细胞胚的再生系数最高可达12.3,有效降低了植株的褐化率和黄化现象,猪屎豆植株的繁殖效率显著提升,可稳定批量生产,能够快速、大批量培育猪屎豆幼苗。上述实验结果对于突破猪屎豆遗传转化瓶颈、推动其功能基因组学与分子育种研究具有重要的理论价值与应用前景。
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