A method for rapid and efficient regeneration of pigskin bean hypocotyls
By using the hypocotyl of *Pseudorasbora spp.* as explants and combining specific culture media and conditions, a highly efficient regeneration system was established, solving the problem of insufficient regeneration systems for *Pseudorasbora spp.* and realizing rapid, efficient regeneration and stable mass production of *Pseudorasbora spp.* seedlings.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
- Filing Date
- 2026-06-02
- Publication Date
- 2026-07-17
AI Technical Summary
In the current technology, an in vitro regeneration system for pig manure beans has not yet been established, which limits its gene editing, genetic transformation and molecular breeding research, and lacks an efficient and stable regeneration system.
Using the hypocotyl of *Pseudorasbora spp.* as explants, and through specific culture medium combinations and culture conditions, including germination, somatic embryo induction, subculture, and rooting culture steps, an efficient regeneration system was established.
It achieves rapid and efficient regeneration of *Phyllostachys edulis* seedlings, improves regeneration rate and propagation efficiency, reduces browning rate and yellowing of plants, and enables stable mass production of *Phyllostachys edulis* seedlings.
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