A freeze-drying protective agent for human diploid cell rabies vaccine and its preparation method and application
Yeast extract prepared by combined induction with hyperosmolar stress and low-temperature cold shock, combined with sucrose and dextran 40, solved the problems of antigen stability and safety during the freeze-drying process of rabies vaccine, and achieved high-efficiency freeze-dried product yield and good reconstitution properties.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- AB&B BIO TECH CO LTD JS
- Filing Date
- 2026-06-16
- Publication Date
- 2026-07-17
AI Technical Summary
Existing rabies vaccine freeze-dried protectants suffer from poor antigen stability, low freeze-drying yield, and insufficient safety. In particular, traditional formulations contain gelatin, which may trigger allergic reactions, and ordinary yeast powder cannot provide effective molecular-level protection.
Yeast extract prepared by combined induction of hyperosmolar stress and low-temperature cold shock, combined with sucrose and dextran 40 as the basic framework, and with the addition of trehalose, extracellular polysaccharides, cold shock proteins and glutathione, forms a variety of protective mechanisms, including replacing water molecules, inhibiting ice crystal recrystallization, stabilizing cell membranes and scavenging free radicals.
It significantly improves the potency stability and finished product quality of vaccines during the freeze-drying process, reduces potency loss during freeze-drying, provides good vitrification support and powder cake structure, ensures controllable reconstitution time, and has high safety.
Smart Images
Figure CN122399042A_ABST