Method for long-term hydrophilic microfluidic chip-dynamic exhaust bubble and multi-outlet flow resistance balance

By combining the anti-bubble injection component and the flow resistance adjustment component, the problems of bubbles and uneven flow resistance in microfluidic chips are solved, achieving long-term hydrophilicity and dynamic bubble removal, thus improving the stability and repeatability of the experiment.

CN122399401APending Publication Date: 2026-07-17XIAMEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
XIAMEN UNIV
Filing Date
2026-06-04
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Air bubbles are difficult to remove from microfluidic chips, affecting fluid dynamics, and uneven flow resistance at multiple outlets leads to inaccurate experimental results and low efficiency.

Method used

Employing anti-bubble injection components, flow resistance adjustment and collection components, and detection components, this system achieves long-term hydrophilicity and balanced flow resistance across multiple outlets through low surface tension solvent pretreatment, seamless liquid bridge connection, real-time monitoring and dynamic bubble removal, and a flow resistance balancing mechanism.

Benefits of technology

This technology achieves long-term hydrophilicity of the microfluidic chip channel, dynamically eliminates air bubbles, maintains experimental continuity, improves experimental repeatability and fluid distribution stability, and is suitable for long-term experimental needs.

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Abstract

本发明涉及一种微流控芯片长效亲水化-动态排气泡与多出口流阻平衡的方法,所述方法包括如下步骤:有机溶剂浸润;缓冲溶液置换并浸没;三通无气泡补液和进样;持续进样状态下连入芯片;采用提高流速、机械扰动方式排除实验过程中的气泡;通过液柱高度法或泵抽取法平衡多出口流阻。本发明提供的方法预处理快速简单,建立了可维持数小时的“抗气泡”亲水状态,在出现气泡时可用极简的物理方法实时动态排除,无需中断实验、拆卸芯片或使用复杂设备,有效解决了多出口流阻不均问题,特别适用于细胞培养、实时显微观察、蛋白质结晶、液滴生成等对气泡极其敏感且需要长时间稳定运行的微流控应用。
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