Immune-enhancing dioscorea polysaccharide and preparation method thereof

By combining electrochemical cell disruption and cryo-electrodialysis with immobilized enzyme membranes, the problems of non-specific cell disruption and structural damage in the extraction of yam polysaccharides were solved, achieving efficient and stable preparation of nano-polysaccharides and enhancing their bioactivity and industrial application potential.

CN122404589APending Publication Date: 2026-07-17中原食品实验室

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
中原食品实验室
Filing Date
2026-06-05
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing methods for extracting yam polysaccharides suffer from problems such as non-specific cell wall disruption, structural damage, low enzymatic hydrolysis efficiency, and unstable product quality, leading to loss of activity and limitations in industrial applications.

Method used

By employing electrochemical cell disruption combined with cryo-electrodialysis and immobilized enzyme membrane technology, and through separation by pulsed electric field and DC electric field, combined with lecithin nano-processing, selective cell disruption and efficient enrichment of yam polysaccharides are achieved.

Benefits of technology

It improves the activity retention rate and purity of polysaccharides, enhances bioavailability, and achieves the stability and efficient extraction of nanoscale products, making it suitable for health foods and medicines for the elderly.

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Abstract

本发明公开一种增强免疫的山药多糖及其制备方法。本发明采用电化学破壁,脉冲电场特异性断裂山药细胞壁Ca2+‑果胶交联网络,实现精准破壁,保护多糖糖苷键与三螺旋结构;结合冷冻电渗析分离,利用电荷与分子量差异实现多糖、蛋白、淀粉的高效分离,无酶、无有机溶剂、无膜污染;再经固定化酶膜耦合精制与高压均质纳米化,得到高分散、高活性纳米山药多糖。本发明产品胃肠道吸收效率与免疫调节活性显著增强,可直接用于特医食品、高端保健品开发。
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