A porous medium microbial conversion continuous flow experimental device for combined regulation of two dissolvable gases and a layered sampling method thereof

By designing a continuous flow experimental device for microbial transformation in porous media, stable input of dual dissolved gases and stratified sample extraction were achieved in porous media. This solved the shortcomings of existing devices in concentration control and sampling processes, and improved the accuracy of experimental results.

CN122405415APending Publication Date: 2026-07-17SOUTHWEAT UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTHWEAT UNIV OF SCI & TECH
Filing Date
2026-06-10
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing experimental setups struggle to achieve stable input and concentration control of dual dissolved gases under continuous flow conditions in porous media, and also make it difficult to extract microbial samples and dissolved gas samples in layers, affecting the accuracy of experimental results.

Method used

Design a continuous flow experimental device for microbial transformation in porous media, comprising four porous media experimental columns, a liquid supply system, a buffer system, a closed liquid drainage system, a stratified microbial sampling structure, and a stratified dissolved gas sampling structure, to achieve joint control of dual dissolved gases and stratified sample extraction.

Benefits of technology

It achieves accurate simulation of microbial transformation processes in porous media under dual dissolved gas conditions, enabling stratified extraction of microbial and dissolved gas samples, reducing the impact of sampling process on gas concentration within the column, and improving the accuracy of experimental results.

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Abstract

本发明提供了一种用于双溶解性气体协同调控的多孔介质微生物转化连续流实验装置及其运行方法,涉及多孔介质中气体迁移转化与微生物过程模拟技术领域。该装置由供液系统、缓冲系统、柱体系统、密闭排液系统、分层微生物取样结构和分层溶解性气体取样结构组成,其中,所述供液系统与柱体系统通过硅胶管连接;柱体系统各层设有多孔承托架,承托架内设有植绒采样头,用于微生物样品的分层采集;各层还连通设有溶解性气体取样器,用于在维持柱内压力平衡的条件下进行取样。所述装置适用于土壤、沉积物等多孔介质中多组分气体耦合过程及微生物响应的模拟研究,可表征复杂组分条件下N2O与DO等溶解性气体在多孔介质中的转化过程。该装置可同步获取不同层位的微生物样品和溶解性气体样品,适合在实验室开展连续模拟与推广应用。
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