A rapid detection device and method for soybean urease activity based on gas expansion
The gas expansion soybean urease activity detection device utilizes the gas generated by the enzymatic reaction to drive the piston, solving the problem of complex matrix interference in on-site testing and achieving rapid and accurate urease activity detection, which is suitable for soil reinforcement projects.
CN122405417APending Publication Date: 2026-07-17ZHENGZHOU UNIV
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Patent Information
- Application Number
- CN202610556220.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-04-24
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
Existing technologies cannot quickly and accurately detect soybean urease activity at the construction site, resulting in poor reinforcement effects or low economic efficiency, and are severely affected by soil matrix interference.
Method used
A rapid detection device for soybean urease activity based on gas expansion is designed. The gas generated by the enzymatic reaction drives the piston to move, and the activity is judged by the piston displacement and color indicator ring, combined with a preset standard curve for detection.
Benefits of technology
It enables rapid and accurate on-site detection of soybean urease activity, avoids soil matrix interference, is easy to operate, low in cost, and suitable for industrial production.
✦ Generated by Eureka AI based on patent content.
Smart Images
Figure CN122405417A_ABST
Abstract
The application provides a kind of based on gas expansion soybean urease activity rapid detection device and detection method.The detection device main body includes reaction cavity, piston, piston rod, scale mark, sample port, base and end cover.The reaction cavity is cylindrical cavity, bottom closed, top open, inside bottom pre-package dry solid acid and urea;The piston is arranged in the reaction cavity, initial position close to the upper side of medicine powder, can slide up under the action of gas pressure;The piston rod is fixedly connected with the piston, extends upward along the axial direction;The scale mark is arranged on the right side of the front of reaction cavity, 0 scale is located at the bottom and the top of piston is aligned;The sample port is arranged at the right side of the bottom of the front of reaction cavity, with medicine powder at the same height, for injecting the enzyme liquid to be measured;The base is arranged at the bottom of the reaction cavity, for fixed support;The end cover is arranged at the end of piston rod.During detection, the enzyme liquid to be measured is injected into the reaction cavity from the side, urease decomposes urea to generate ammonium carbonate, ammonium carbonate reacts with solid acid to release carbon dioxide gas, gas expansion pushes the piston to move upward, according to the displacement distance of piston after fixed time, the urease activity is determined by comparing with standard curve.The application converts enzymatic reaction into intuitive mechanical displacement, is not interfered by sample color and turbidity, has simple structure, low cost, and is suitable for soil reinforcement engineering site rapid detection.
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