A rapid genetic transformation method of litsea cubeba adventitious buds with anti-browning
By using adventitious buds as recipients in Litsea cubeba plants for genetic transformation, combined with polyphenol oxidase treatment and a specific culture medium, the problem of oxidative browning was solved, achieving efficient and stable genetic transformation, improving the transformation rate and shortening the cycle.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- HUNAN ACAD OF FORESTRY
- Filing Date
- 2026-06-04
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies for genetic transformation of Litsea cubeba suffer from severe oxidative browning, resulting in low transformation rates, long cycles, and poor genetic stability, making it difficult to establish an efficient genetic transformation system.
By directly using adventitious buds as recipients and combining them with polyphenol oxidase treatment, adventitious buds proliferate and are infected by Agrobacterium through disinfection and specific culture medium, avoiding oxidative browning, shortening the cycle, and improving transformation rate and genetic stability.
It effectively reduced the oxidative browning rate to 16.19%, achieved a conversion rate of 18.67%, shortened the cycle to 2 months, eliminated somatic asexual mutations, and provided a highly efficient and stable genetic transformation platform.
Smart Images
Figure CN122405718A_ABST