Recombinant trichoderma harzianum for blocking competitive metabolic branch to improve gibberellin production and construction method thereof
By establishing an efficient gene knockout system for Fumigia fujikuro, a recombinant Fumigia fujikuro with p450-3 gene knockout was successfully constructed, solving the problem of limited gibberellin production in existing technologies and realizing the increase in gibberellin GA4 production and the targeted redistribution of metabolic flux.
CN122445486APending Publication Date: 2026-07-24ZHEJIANG FORESTRY UNIVERSITY
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Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- ZHEJIANG FORESTRY UNIVERSITY
- Filing Date
- 2026-06-10
- Publication Date
- 2026-07-24
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Figure CN122445486A_ABST
Abstract
The application discloses a recombinant Gibberella fujikuroi for blocking competitive metabolic branch to improve gibberellin yield and a construction method thereof, and belongs to the technical field of industrial microorganisms.The recombinant Gibberella fujikuroi provided by the application knocks out a p450-3 gene, the GA4 yield of the recombinant strain is increased to 718.6 mg / L from 585.9 mg / L of the original strain, is increased by 22.6%, and the growth characteristics of the strain are not significantly affected.Through blocking the 13-hydroxylation competitive branch, the application realizes the redirection of metabolic flow to non-13-hydroxylation product GA4 / 7, provides an efficient chassis strain and a construction method for directional production of gibberellin components, and has important industrial application value.
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