Anti-acne propionibacterium compound essential oil, preparation method and application thereof

Abstract

This invention discloses an anti-acne Propionibacterium acnes compound essential oil, its preparation method, and its application. The anti-acne Propionibacterium acnes compound essential oil is composed of the following components in the indicated mass percentages: 22%~28% helichrysum essential oil, 22%~28% sweet basil essential oil, 22%~28% eucalyptol rosemary essential oil, and 22%~28% true lavender essential oil. This invention achieves highly effective antibacterial activity against Propionibacterium acnes through a precise selection of four plant essential oils and a scientifically formulated compound. It also possesses multiple benefits including anti-inflammatory properties, promotion of skin cell regeneration, and a natural fragrance. Furthermore, the product is natural, safe, and has no side effects, is suitable for various skin types, and can be directly applied to the preparation of various acne-removing skincare products.

Description

Technical Field

[0001] This invention relates to the field of antibacterial technology, specifically to an anti-acne Propionibacterium acnes compound essential oil, its preparation method, and its application. Background Technology

[0002] Propionibacterium acnes is the core pathogen causing acne and pimples. This bacterium is an anaerobic or facultative anaerobic bacterium that mainly lives in human hair follicles and sebaceous glands. During its metabolism, it produces small molecule polypeptides that attract phagocytes to the parasitic site, release hydrolytic enzymes and inflammatory mediators, induce local inflammatory reactions in the skin, destroy the structure of sebaceous glands, and thus form skin problems such as pimples, papules, erythema, pustules, nodules, and cysts. In severe cases, it can also lead to scarring, hair loss, and even enter the human body through skin incisions, causing systemic infections such as bacteremia and endocarditis.

[0003] Currently, antibacterial and acne-removing products targeting Propionibacterium acnes on the market are mainly divided into two categories: chemical antibacterial and acne-removing products and natural essential oil-based antibacterial and acne-removing products. The relevant technologies and principles are as follows: Chemical antibacterial and acne-removing products: These products use antibiotics, benzoyl peroxide, and retinoids as core active ingredients and are widely used in skincare products such as facial cleansers, acne creams, and acne serums. Their antibacterial mechanism mainly involves interfering with the protein synthesis of Propionibacterium acnes, disrupting bacterial cell metabolic pathways, or reducing the bacterial survival environment by regulating sebaceous gland secretion. However, these products can only achieve a single antibacterial or oil-controlling effect and cannot simultaneously address skin inflammation repair.

[0004] Natural essential oil-based antibacterial and acne-fighting products: These products utilize the natural antibacterial properties of plant essential oils to achieve their antibacterial effect. Research on the antibacterial properties of plant essential oils began in 1881. Subsequent domestic and international studies have confirmed that essential oils such as mustard, cinnamon, garlic, thyme, and lavender have varying degrees of inhibitory effects on a variety of pathogenic bacteria, and some essential oils also exhibit synergistic antibacterial effects. Their antibacterial mechanism mainly involves disrupting the bacterial cell wall and cell membrane, interfering with the bacterial energy metabolism system, and inhibiting bacterial biofilm formation. Furthermore, natural essential oils also possess certain skincare and fragrance benefits, gradually becoming a research direction in the field of acne treatment and skincare.

[0005] However, chemical antibacterial acne treatment products have the following drawbacks: long-term use of antibiotic ingredients can easily lead to drug resistance in Propionibacterium acnes, significantly reducing the subsequent antibacterial and acne-removing effects; ingredients such as benzoyl peroxide and retinoids are highly irritating, and after application, they can easily cause secondary skin damage such as redness, dryness, and peeling in people with sensitive skin or damaged skin barriers; moreover, these products have a single function, only able to inhibit bacteria or control oil, and cannot repair the inflamed areas caused by acne, easily leaving acne marks and scars. Natural essential oil-based antibacterial and acne-removing products have the following drawbacks: Single plant essential oils have limited antibacterial efficiency against Propionibacterium acnes, and most essential oils require high doses to achieve a basic antibacterial effect; excessive addition can burden the skin. Existing compound essential oil formulas lack specificity, failing to screen essential oils based on the anaerobic characteristics and inflammatory mechanisms of Propionibacterium acnes, and not fully utilizing the synergistic antibacterial effects of different essential oils, resulting in generally low antibacterial rates that cannot meet the standards for highly effective antibacterial treatment. Some compound essential oil formulas only consider antibacterial efficacy, neglecting anti-inflammatory and skin cell regeneration needs, leading to frequent acne recurrence after treatment. Summary of the Invention

[0006] The purpose of this invention is to provide an anti-Propionibacterium acnes compound essential oil, its preparation method, and its application. This invention achieves highly effective antibacterial activity against Propionibacterium acnes through a precise selection of four plant essential oils and a scientific compounding technique. It also possesses multiple benefits, including anti-inflammatory properties, promotion of skin cell regeneration, and natural fragrance. Furthermore, the product is natural, safe, and has no side effects, is suitable for various skin types, and can be directly applied to the preparation of various acne-removing skincare products. This invention solves the problems of existing acne-removing products, such as chemical products easily developing drug resistance and strong irritation, and natural essential oil products having low antibacterial efficiency, lack of targeted formulation, and single efficacy.

[0007] In order to achieve the above-mentioned objectives of the present invention, the following technical solution is adopted:

[0008] The first aspect of this invention provides an anti-acne Propionibacterium acnes compound essential oil, wherein the anti-acne Propionibacterium acnes compound essential oil is composed of the following components in weight percentage:

[0009] Helichrysum essential oil 22%~28%, sweet basil essential oil 22%~28%, eucalyptol rosemary essential oil 22%~28%, and true lavender essential oil 22%~28%.

[0010] Preferably, the anti-acne Propionibacterium acnes compound essential oil is composed of the following components in weight percentage:

[0011] 25% Helichrysum essential oil, 25% Sweet basil essential oil, 25% Eucalyptol rosemary essential oil, and 25% True Lavender essential oil.

[0012] Preferably, the helichrysum essential oil, sweet basil essential oil, eucalyptol rosemary essential oil, and true lavender essential oil are obtained by distillation extraction of helichrysum, sweet basil, eucalyptol rosemary, and true lavender, respectively.

[0013] A second aspect of the present invention provides a method for preparing the above-mentioned anti-acne Propionibacterium acnes compound essential oil, the preparation method comprising the following steps:

[0014] (a) Weigh each component according to its mass percentage;

[0015] (b) Mix and homogenize the components, let them stand, and then fill them to obtain the anti-acne Propionibacterium compound essential oil.

[0016] Preferably, the homogenization is performed by stirring at a speed of 40-60 rpm for 8-15 minutes; the settling time is 25-40 minutes.

[0017] The third aspect of this invention provides the application of the above-mentioned anti-acne Propionibacterium acnes compound essential oil in the preparation of acne-removing skin care products.

[0018] Preferably, the acne-removing skincare products include facial cleanser, facial mask, acne cream, acne serum, shower gel, body lotion, and bath bomb.

[0019] The fourth aspect of the present invention provides an acne-removing skin care product, the acne-removing skin care product comprising a therapeutically effective amount of the anti-acne Propionibacterium acnes compound essential oil.

[0020] Preferably, the mass content of the anti-acne Propionibacterium acnes compound essential oil in the acne-removing skin care product is 1% to 5%.

[0021] Compared with the prior art, the beneficial effects of the present invention include at least the following:

[0022] This invention achieves highly effective antibacterial activity against Propionibacterium acnes by precisely selecting four plant essential oils and scientifically compounding them. It also possesses multiple benefits including anti-inflammatory properties, promotion of skin cell regeneration, and a natural fragrance. The product is natural, safe, and has no side effects, is suitable for various skin types, and can be directly applied to the preparation of various acne-removing skincare products. This invention solves the problems of existing acne products, such as chemical-based products easily developing drug resistance and strong irritation, and natural essential oil products having low antibacterial efficiency, lack of targeted formulation, and single efficacy. Specifically, verified by a professional third-party testing institution, the compound essential oil of this invention achieves an antibacterial rate of >99.9% after 6 hours of contact with Propionibacterium acnes, far exceeding that of existing natural essential oil-based acne products. It can quickly kill Propionibacterium acnes in hair follicles and sebaceous glands, rapidly controlling acute acne inflammation. Moreover, the antibacterial efficiency of the compound essential oil prepared in different batches all reaches the standard of >99.9%, with no difference in efficacy, facilitating industrial production. The large-scale industrial production and marketing of this invention solves the problems of inconsistent formulas and unstable effects of existing essential oil products. The compound essential oil of this invention is a 100% pure plant essential oil blend, free of antibiotics, benzoyl peroxide, retinoic acid, and other chemical ingredients. It is free of solvent dilution and artificial additives, and long-term use will not lead to drug resistance in Propionibacterium acnes. It also has extremely low irritation and is suitable for various skin types, including sensitive skin, skin with damaged skin barriers, and healthy skin, thus solving the problems of drug resistance and irritation associated with chemical acne treatments. Furthermore, the four essential oils are precisely selected and scientifically blended to form a synergistic antibacterial system, significantly improving antibacterial efficiency. It also possesses five major functions: antibacterial, anti-inflammatory, promotes skin cell regeneration, controls oil, and provides a natural fragrance. It can solve problems such as acne, papules, and pustules caused by Propionibacterium acnes in one go, repairing inflamed and damaged skin, and preventing acne marks and scars, thus solving the problem of single-function products in existing products.

[0023] Furthermore, this invention creates a natural grassy fragrance through the blending of four essential oils. It initially bursts with the scent of fresh, tender green grass, followed by the rounded sweetness of sweet basil, and the base notes return to a green grassy aroma with a slight bitterness. It lacks the pungent smell of artificial fragrances, providing a pleasant olfactory experience during use. Simultaneously, the essential oil molecules freshen the surrounding air, achieving a dual effect of skincare and fragrance. It can be added at a ratio of 1% to 5% to various facial and body skincare products, including facial cleansers, cleansing masks, acne creams, acne serums, shower gels, body lotions, bath bombs, etc. It exhibits good compatibility with various skincare bases such as oil-based, oil-in-water / oil-in-water emulsions, and creams, requiring no modification and demonstrating strong adaptability. In addition, all raw materials are obtained from natural plants through distillation and extraction, are biodegradable, and cause no environmental pollution during production and use, meeting current consumer demand for natural, environmentally friendly, and plant-based skincare products, resulting in high market acceptance.

[0024] The compound essential oil preparation process of this invention only requires three steps: weighing raw materials, mixing and stirring, and static filling. It does not require special modification equipment and can be carried out in a conventional sterile cosmetic production workshop. The production process is simple and easy to operate, which reduces the production and manufacturing cost. Detailed Implementation

[0025] The embodiments of the technical solution of the present invention will be described in detail below with reference to the examples. The following embodiments are only used to illustrate the technical solution of the present invention more clearly, and are therefore only examples, and should not be used to limit the scope of protection of the present invention.

[0026] It should be noted that, unless otherwise stated, the technical or scientific terms used in this application should have the ordinary meaning as understood by those skilled in the art to which this invention pertains.

[0027] This invention provides an anti-acne Propionibacterium acnes compound essential oil, which is composed of the following components in weight percentage:

[0028] Helichrysum essential oil 22%~28%, sweet basil essential oil 22%~28%, eucalyptol rosemary essential oil 22%~28%, and true lavender essential oil 22%~28%.

[0029] In one embodiment, the anti-acne Propionibacterium acnes compound essential oil is composed of the following components in weight percentage:

[0030] 25% Helichrysum essential oil, 25% Sweet basil essential oil, 25% Eucalyptol rosemary essential oil, and 25% True Lavender essential oil.

[0031] In one embodiment, the helichrysum essential oil, sweet basil essential oil, eucalyptol rosemary essential oil, and true lavender essential oil are obtained by distillation extraction of helichrysum, sweet basil, eucalyptol rosemary, and true lavender, respectively.

[0032] Another embodiment of the present invention provides a method for preparing the above-mentioned anti-acne Propionibacterium acnes compound essential oil, the preparation method comprising the following steps:

[0033] (a) Weigh each component according to its mass percentage;

[0034] (b) Mix and homogenize the components, let them stand, and then fill them to obtain the anti-acne Propionibacterium compound essential oil.

[0035] In one embodiment, the homogenization is performed by stirring at a speed of 40-60 rpm for 8-15 minutes; the settling time is 25-40 minutes.

[0036] Another embodiment of the present invention provides the application of the above-mentioned anti-acne Propionibacterium acnes compound essential oil in the preparation of acne-removing skin care products.

[0037] In one embodiment, the acne-removing skincare products include facial cleanser, facial mask, acne cream, acne serum, shower gel, body lotion, and bath bomb.

[0038] Another embodiment of the present invention provides an acne-removing skin care product, the acne-removing skin care product comprising a therapeutically effective amount of the anti-acne Propionibacterium acnes compound essential oil.

[0039] In one embodiment, the mass content of the anti-acne Propionibacterium acnes compound essential oil in the acne-removing skin care product can be any value between 1% and 5%.

[0040] The raw materials used in the following embodiments are as follows:

[0041] Helichrysum essential oil: It is extracted from the whole flowering helichrysum plant (Helichrysum italicum) using steam distillation.

[0042] Sweet basil essential oil: It is extracted from the whole flowering sweet basil plant (Ocimum basilicum) using steam distillation.

[0043] Eucalyptol rosemary essential oil: derived from the whole flowering plant of eucalyptol rosemary (Rosmarinus officinalis), extracted using steam distillation;

[0044] True lavender essential oil: It is extracted from the fresh terminal flower spikes of the whole true lavender (Lavandula angustifolia) plant using steam distillation.

[0045] Example 1

[0046] This embodiment is an anti-acne Propionibacterium acnes compound essential oil, which is composed of the following components in weight percentage:

[0047] It contains 22% helichrysum essential oil, 22% sweet basil essential oil, 28% eucalyptol rosemary essential oil, and 28% true lavender essential oil.

[0048] The preparation method of the above-mentioned anti-acne Propionibacterium acnes compound essential oil includes the following steps:

[0049] (a) Weigh each component according to its mass percentage;

[0050] (b) Mix the components and stir at 40 rpm for 15 min, let stand for 25 min, and then fill into the container to obtain the anti-acne Propionibacterium compound essential oil.

[0051] Example 2

[0052] This embodiment is an anti-acne Propionibacterium acnes compound essential oil, which is composed of the following components in weight percentage:

[0053] It contains 28% helichrysum essential oil, 28% sweet basil essential oil, 22% eucalyptol rosemary essential oil, and 22% true lavender essential oil.

[0054] The preparation method of the above-mentioned anti-acne Propionibacterium acnes compound essential oil includes the following steps:

[0055] (a) Weigh each component according to its mass percentage;

[0056] (b) Mix the components and stir at 60 rpm for 8 minutes, let stand for 40 minutes, and then fill into the container to obtain the anti-acne Propionibacterium compound essential oil.

[0057] Example 3

[0058] This embodiment is an anti-acne Propionibacterium acnes compound essential oil, which is composed of the following components in weight percentage:

[0059] 25% Helichrysum essential oil, 25% Sweet basil essential oil, 25% Eucalyptol rosemary essential oil, and 25% True Lavender essential oil.

[0060] The preparation method of the above-mentioned anti-acne Propionibacterium acnes compound essential oil includes the following steps:

[0061] (a) Weigh each component according to its mass percentage;

[0062] (b) Mix the components and stir at 50 rpm for 10 min, let stand for 30 min, and then fill into the container to obtain the anti-acne Propionibacterium compound essential oil.

[0063] Example 4

[0064] This embodiment is an acne-removing skin care product, which includes a therapeutically effective amount of the anti-acne Propionibacterium acnes compound essential oil; the mass content of the anti-acne Propionibacterium acnes compound essential oil in the acne-removing skin care product is 2%.

[0065] Experimental Example

[0066] Obtain the anti-acne Propionibacterium acnes compound essential oil prepared in Example 3;

[0067] Test strain: Propionibacterium acnes, preservation number ATCC 6919;

[0068] Culture media: Reubfircated Clostridial Medium (RCM); Reubfircated Clostridial Agar (RCA); Neutralizing agent (Polysorbate 80 / Lecithin);

[0069] Experimental Groups:

[0070] Experimental group: Inoculated strains with compound essential oils as described in Example 3.

[0071] Control group: The strain was inoculated with 70% alcohol to confirm that the test strain could be killed;

[0072] Blank control group: concentration of the original inoculated strain.

[0073] Preparation of bacterial solution:

[0074] The test strain was prepared into a culture medium with a bacterial concentration of 10⁶ CFU / ml.

[0075] Antibacterial test:

[0076] Take 950 μL of the compound essential oil and add it to 50 μL of bacterial solution. Mix and let it contact at room temperature for 6 hours.

[0077] This mixture was then serially diluted 10-fold with a neutralizing agent, and 1000 μL of each dilution was cultured using a spread plate method. The control group (70% ethanol) was treated in the same manner. The blank control group was treated without any treatment and remained at the original bacterial concentration.

[0078] All culture trays were incubated at 32.5±2.5℃ for 6 days.

[0079] Experimental Analysis:

[0080] After the culture is completed, the colony count is calculated, and the inhibition rate is calculated according to the following formula.

[0081] Inhibition rate (%) = (number of colonies in blank control group - number of colonies in experimental group) / number of colonies in blank control group × 100%.

[0082] The results are shown in Table 1:

[0083] Table 1

[0084] Group Colony count (cfu / mL) Antibacterial rate (%) Blank control group <![CDATA[1.4×10 6 ]]> -- Control group (alcohol) ＜10 ＞99.9 Example 3 ＜10 ＞99.9

[0085] As shown in Table 1:

[0086] The compound essential oil prepared by this invention has excellent antibacterial effect, with an antibacterial rate of over 99.9%.

[0087] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention, and they should all be covered within the scope of the claims and specification of the present invention.

Claims

1. A compound essential oil for combating Propionibacterium acnes, characterized in that, The anti-acne Propionibacterium acnes compound essential oil is composed of the following components in weight percentage: Helichrysum essential oil 22%~28%, sweet basil essential oil 22%~28%, eucalyptol rosemary essential oil 22%~28%, and true lavender essential oil 22%~28%.

2. The anti-acne Propionibacterium acnes compound essential oil according to claim 1, characterized in that, The anti-acne Propionibacterium acnes compound essential oil is composed of the following components in weight percentage: 25% Helichrysum essential oil, 25% Sweet basil essential oil, 25% Eucalyptol rosemary essential oil, and 25% True Lavender essential oil.

3. The anti-acne Propionibacterium acnes compound essential oil according to claim 1 or 2, characterized in that, The helichrysum essential oil, sweet basil essential oil, eucalyptol rosemary essential oil, and true lavender essential oil were obtained by distillation extraction of helichrysum, sweet basil, eucalyptol rosemary, and true lavender, respectively.

4. The method for preparing the anti-acne Propionibacterium acnes compound essential oil according to any one of claims 1 to 3, characterized in that, The preparation method includes the following steps: (a) Weigh each component according to its mass percentage; (b) Mix and homogenize the components, let them stand, and then fill them to obtain the anti-acne Propionibacterium compound essential oil.

5. The preparation method according to claim 4, characterized in that, The homogenization is performed by stirring at a speed of 40-60 rpm for 8-15 minutes; the settling time is 25-40 minutes.

6. The application of the anti-acne Propionibacterium acnes compound essential oil according to any one of claims 1 to 3 in the preparation of acne-removing skin care products.

7. The application according to claim 6, characterized in that, The acne-removing skincare products include facial cleanser, face mask, acne cream, acne serum, shower gel, body lotion, and bath bomb.

8. An acne-removing skincare product, characterized in that, The acne-removing skincare product includes a therapeutically effective amount of the anti-acne Propionibacterium acnes compound essential oil as described in any one of claims 1 to 3.

9. The acne-removing skincare product according to claim 8, characterized in that, The mass content of the anti-acne Propionibacterium acnes compound essential oil in the acne-removing skin care product is 1%~5%.