A ferulic acid-amino silica gel surface grafted molecularly imprinted polymer, a preparation method and application thereof

Abstract

This invention belongs to the field of traditional Chinese medicine and feed analysis and detection technology, and discloses a ferulic acid-amino silica gel surface-grafted molecularly imprinted polymer, its preparation method, and its application. The method involves dissolving ferulic acid in a solvent, adding amino silica gel and a functional monomer containing double bonds, and performing a prepolymerization reaction at 0-10℃ to form a prepolymer. Under a protective atmosphere, a crosslinking agent and an initiator are added to the prepolymer, and the polymerization reaction is carried out with stirring at 40-90℃. The resulting product is centrifuged and eluted to remove the ferulic acid template, washed until neutral, and then vacuum dried to obtain the ferulic acid-amino silica gel surface-grafted molecularly imprinted polymer. This invention uses amino-modified silica gel as a carrier, and the preparation process is simple and controllable with low raw material costs. The prepared imprinted polymer has a fast mass transfer rate, high adsorption efficiency, and exhibits excellent molecular recognition ability and selective enrichment performance for ferulic acid, making it suitable for the separation and detection of ferulic acid in complex biological matrices.

Description

Technical Field

[0001] This invention belongs to the field of analytical chemistry and the analysis and detection technology of traditional Chinese medicine and feed. More specifically, it relates to a ferulic acid-amino silica gel surface grafted molecularly imprinted polymer, its preparation method and application. Background Technology

[0002] Ferulic acid (FA), chemically known as 4-hydroxy-3-methoxycinnamic acid, is a phenolic acid compound widely found in nature. It is one of the characteristic active ingredients in many traditional Chinese medicines such as Angelica sinensis, Ligusticum chuanxiong, and Cimicifuga foetida. In the Pharmacopoeia of the People's Republic of China (2020 edition), the content of ferulic acid in Ligusticum chuanxiong is explicitly stipulated to be no less than 0.1%.

[0003] Ferulic acid, with its significant antioxidant, anti-inflammatory, antibacterial, and hepatoprotective activities, shows broad application prospects in pharmaceuticals, food, cosmetics, and animal feed. However, the separation and detection of ferulic acid in complex matrices still face challenges. Extraction of ferulic acid from traditional Chinese medicine mainly uses organic solvents such as methanol. Traditional purification and enrichment techniques lack specificity, and numerous interfering components significantly impact ferulic acid detection, making accurate determination difficult. Therefore, developing efficient, specific, and economical ferulic acid separation and detection technologies will not only promote its application in the quality control of traditional Chinese medicine and in pharmaceuticals, food, and animal feed, but also provide crucial technical support for the precise analysis of active ingredients in complex matrices. Summary of the Invention

[0004] This invention aims to overcome the shortcomings and disadvantages of existing technologies, and its objective is to provide a molecularly imprinted polymer for grafting ferulic acid-amino silica gel surfaces. This polymer exhibits good selective adsorption properties for target analytes.

[0005] Another object of the present invention is to provide a method for preparing the above-mentioned ferulic acid-amino silica gel surface grafted molecularly imprinted polymer.

[0006] Another object of the present invention is to provide the application of the above-mentioned ferulic acid-amino silica gel surface grafted molecularly imprinted polymer.

[0007] The above-mentioned objectives of this invention are achieved through the following technical solutions: A ferulic acid-aminosilica gel surface-grafted molecularly imprinted polymer is prepared by dissolving ferulic acid in a solvent, adding aminosilica gel and a monomer containing double bonds, and prepolymerizing at 0-10°C. Under a protective atmosphere, the resulting prepolymer is added to a crosslinking agent and an initiator, and polymerized at 40-90°C with stirring. The obtained product is centrifuged and eluted to remove the ferulic acid template, washed until neutral, and then vacuum dried.

[0008] Preferably, the solvent is one or more of acetonitrile, methanol, or ethyl acetate; and the double-bonded functional monomer is one or more of 2-vinylpyridine (2-VP), 4-vinylpyridine (4-VP), acrylamide (AM), methacrylic acid (MAA), or acrylic acid (AA).

[0009] Preferably, the initiator is azobisisobutyronitrile, azobisisoheptanenitrile, azobisisovalerate, benzoyl peroxide, potassium persulfate, or ammonium persulfate; and the crosslinking agent is one or more of trimethylolpropane triacrylate (TMPTMA), ethylene glycol diglycidyl ether (EGDE), or ethylene glycol dimethacrylate (EGDMA).

[0010] Preferably, the molar ratio of ferulic acid to the double-bonded functional monomer is 1:(3~10); the mass ratio of aminosilicone to ferulic acid is (0.015~0.06):(0.1~0.3).

[0011] Preferably, the mass ratio of the initiator to the crosslinking agent is (5~15):(250~300).

[0012] The preparation method of the ferulic acid-amino silica gel surface grafted molecularly imprinted polymer includes the following specific steps: S1. Dissolve ferulic acid in a solvent, add amino silica gel and a monomer containing double bonds, and prepolymerize at 0~10℃ to form a prepolymer; S2. Under a protective atmosphere, a crosslinking agent and an initiator are added to the prepolymer, and the polymerization reaction is carried out by stirring at 40~90℃. The obtained product is centrifuged and eluted to remove the ferulic acid template, washed until neutral, and dried under vacuum to obtain a ferulic acid-aminosilicone surface-grafted molecularly imprinted polymer.

[0013] Preferably, the prepolymerization reaction in step S1 takes 2 to 12 hours.

[0014] Preferably, the protective atmosphere in step S2 is helium, argon, or nitrogen.

[0015] Preferably, the polymerization reaction in step S2 takes 6 to 24 hours. The application of the ferulic acid-aminosilicone surface-grafted molecularly imprinted polymer in the selective recognition or detection of ferulic acid.

[0016] A method for preparing an organic phase of a ferulic acid-amino silica gel surface grafted with a molecularly imprinted polymer includes the following specific steps: S1. Ferulic acid is dissolved in acetonitrile, amino silica gel and functional monomers are added, and prepolymerized at 0-10℃; S2. Add crosslinking agent and initiator, and stir the polymerization reaction at 40-90℃ under a protective atmosphere. After centrifugation, the ferulic acid template is removed by washing, and the product is washed and vacuum dried to obtain spherical ferulic acid-amino silica gel surface grafted molecularly imprinted polymer.

[0017] Preferably, the functional monomer in step S1 is a compound containing a double bond, such as 2-vinylpyridine (2-VP), 4-vinylpyridine (4-VP), acrylamide (AM), methacrylic acid (MAA), or acrylic acid (AA).

[0018] The ferulic acid-amino silica gel surface-grafted molecularly imprinted polymer of the present invention can selectively recognize ferulic acid template molecules and their structural analogs; wherein the ferulic acid-amino silica gel surface-grafted molecularly imprinted polymer has uniform particle size, high pressure resistance, large adsorption capacity, and fast mass transfer rate, and the surface has three-dimensional pores and specifically binding active sites that match the ferulic acid template molecules, which can be used for the selective separation and enrichment of trace amounts of ferulic acid in complex samples, and the regenerated ferulic acid-amino silica gel surface-grafted molecularly imprinted polymer can be repeatedly recycled.

[0019] Compared with the prior art, the present invention has the following beneficial effects: 1. The preparation process of the ferulic acid-amino silica gel surface grafted molecularly imprinted polymer of the present invention is simple and low in cost. The obtained ferulic acid-amino silica gel surface grafted molecularly imprinted polymer has excellent properties such as uniform particle size (3~12 μm), high strength (pressure resistance 200~400 bar), large adsorption capacity (30~50 mg / g), and fast mass transfer rate (1~20 min).

[0020] 2. This invention utilizes the combined action of a crosslinking agent and an initiator to prepare a grafted molecularly imprinted polymer on the surface of ferulic acid-amino silica gel. This imprinted polymer possesses binding sites with specific recognition properties for ferulic acid, exhibiting excellent selectivity and adsorption effects. Combined with high-performance liquid chromatography (HPLC), it can be used for the selective separation and enrichment of ferulic acid (concentration of 0.25~50 mg / g) in complex samples, demonstrating broad application prospects. Attached Figure Description

[0021] Figure 1 Infrared spectra of amino silicone (A) and ferulic acid-amino silicone surface-grafted molecularly imprinted polymer (B) of Example 1; Figure 2 Scanning electron microscope image of the ferulic acid-amino silica gel surface grafted with molecularly imprinted polymer in Example 1; Figure 3High-performance liquid chromatography (HPLC) chromatograms of ferulic acid desorbed from ferulic acid by molecularly imprinted polymer (A) grafted onto ferulic acid-amino silica gel surface in Example 1 and non-molecularly imprinted polymer (B) in Comparative Example 1. Figure 4 The adsorption effects of ferulic acid-amino silica gel surface-grafted molecularly imprinted polymers prepared with different monomers in Examples 1-5 and non-molecularly imprinted polymers in Comparative Examples 1-5 are compared. Figure 5 The adsorption effects of ferulic acid-amino silica gel surface-grafted molecularly imprinted polymers and non-molecularly imprinted polymers prepared with different crosslinking agents for Examples 1, 6 and 7. Detailed Implementation

[0022] The present invention will be further described below with reference to specific embodiments, but these should not be construed as limiting the invention. Unless otherwise specified, the technical means used in the embodiments are conventional means well known to those skilled in the art. Unless otherwise specified, the reagents, methods, and equipment used in the present invention are conventional reagents, methods, and equipment in this technical field.

[0023] The amino silicone used in this embodiment of the invention was purchased from Silicycle, Canada; ferulic acid was purchased from Shanghai McLean Biochemical Technology Co., Ltd.; and methacrylic acid was purchased from Beijing Bailingwei Technology Co., Ltd.

[0024] Example 1

[0025] 1. Add 30 mL of acetonitrile, 0.2 g of aminosilicone, 0.03 g of ferulic acid and 50 μL of methacrylic acid to a 50 mL round-bottom flask, and prepolymerize at 4 °C for 6 h to form a prepolymer; 2. Add 0.24 mL of crosslinking agent ethylene glycol diglycidyl ether (EGDE) and 10 mg of azobisisobutyronitrile to the prepolymer, and stir and polymerize at 60 °C for 24 h under nitrogen protection. The product is eluted with a methanol solution of 20% acetic acid (v / v) to remove ferulic acid template molecules, and then washed with methanol and water until neutral. The product is then dried under vacuum at 60 °C to obtain a ferulic acid-aminosilicone surface grafted molecularly imprinted polymer.

[0026] Method for determining the adsorption capacity of the synthesized polymer: Several 10 mg portions of ferulic acid-aminosilica surface-grafted molecularly imprinted polymer were weighed and placed in 5 mL centrifuge tubes. 2 mL of 1.0 mg / mL ferulic acid methanol solution was added to each tube, and the mixture was shaken in a water bath at 25 °C for 24 h. All sample solutions were centrifuged (centrifugal force 8000 (×g)) for 5 min, and the supernatant was collected. The supernatant was filtered through a 0.22 μm microporous membrane, and the ferulic acid content in the supernatant was determined by high-performance liquid chromatography (HPLC). The adsorption capacity of the ferulic acid-aminosilica surface-grafted molecularly imprinted polymer for ferulic acid was then calculated.

[0027] Comparative Example 1 Unlike Example 1, ferulic acid template molecules were not added in step 1 to obtain a non-molecularly imprinted polymer.

[0028] The imprinting factor (IF) is the ratio of the binding ability of an imprinted polymer (MIP) to a non-imprinted polymer (NIP) for a template molecule. IF = MIP binding amount / NIP binding amount, and is used to measure the selective recognition ability of a molecularly imprinted polymer for a target molecule. Under the same testing conditions, the ferulic acid-aminosilica surface-grafted molecularly imprinted polymer prepared in Example 1 and the non-molecularly imprinted polymer prepared in Comparative Example 1 showed adsorption capacities of 46.5 mg / g and 21.9 mg / g for ferulic acid, respectively, with an imprinting factor of 2.12.

[0029] Figure 1 The images show the infrared absorption spectra of amino silicone and ferulic acid-amino silicone surface-grafted molecularly imprinted polymers in Example 1. In these spectra, A represents amino silicone, and B represents the ferulic acid-amino silicone surface-grafted molecularly imprinted polymer. Figure 1 It can be determined that 3440.87 cm -1 1638.72 cm -1 and 1093.44 cm -1 The absorption at 809.96 cm⁻¹ is due to the stretching vibration of Si-OH. -1 and 464.76 cm -1 The absorption peak at 2989.12 cm⁻¹ is mainly a Si-O-Si absorption peak. -1 1723.06 cm -1 An absorption peak appears. Among them, the peak is at 2989.12 cm⁻¹. -1 The absorption peak is due to the CH stretching vibration of -CH3 or -CH2-, at 1723.06 cm⁻¹. -1 The peak represents the carbonyl C=O stretching vibration of methacrylic acid, indicating that during the imprinting process, the functional monomer methacrylic acid was successfully grafted onto the surface of amino silicone, and the silicone surface was coated with ferulic acid-amino silicone surface-grafted molecular imprinted polymer.

[0030] Figure 2 This is a scanning electron micrograph of the ferulic acid-amino silica gel surface-grafted molecularly imprinted polymer prepared in Example 1. From... Figure 2 It can be seen that the surface of the amino silicone ball is rough and not smooth, with scaly polymers irregularly distributed on the surface of the amino silicone ball, and irregular molecularly imprinted polymers attached to the surface of the amino silicone ball. Both of these completely encapsulate the entire amino silicone ball.

[0031] Figure 3The figures show high-performance liquid chromatograms (HPLC) of ferulic acid in the methanol extract of *Ligusticum chuanxiong* after desorption from the molecularly imprinted polymer grafted onto the ferulic acid-aminosilica surface in Example 1 and the non-imprinted polymer in Comparative Example 1; where A represents the imprinted polymer and B represents the non-imprinted polymer. Figure 3 It can be seen that the ferulic acid-amino silica gel surface grafted molecularly imprinted polymer has a large adsorption capacity for ferulic acid, and the concentration of ferulic acid in the desorption solution is significantly higher than that of the non-imprinted polymer.

[0032] Example 2

[0033] The difference from Example 1 is that in step 1, 30 mL of acetonitrile, 0.2 g of aminosilicone, 0.03 g of ferulic acid and 64 μL of 4-vinylpyridine monomer were added to a 50 mL round-bottom flask, and prepolymerized at 4 °C for 6 h. The resulting product was eluted with acetic acid / methanol (v / v=1:1) to obtain a ferulic acid-aminosilicone surface grafted molecularly imprinted polymer.

[0034] Comparative Example 2 The difference from Example 2 is that ferulic acid template molecules are not added in step 1, resulting in a non-molecularly imprinted polymer.

[0035] Under the same test conditions, Example 2 and Comparative Example 2 prepared ferulic acid-aminosilica surface-grafted molecularly imprinted polymers and non-imprinted polymers, respectively, and the adsorption capacities of ferulic acid were 46.35 mg / g and 22.90 mg / g, respectively, with an imprinting factor of 2.02.

[0036] Example 3

[0037] The difference from Example 1 is that in step 1, 30 mL of acetonitrile, 0.2 g of aminosilicone, 0.03 g of ferulic acid and 64 μL of 2-vinylpyridine monomer were added to a 50 mL round-bottom flask, and prepolymerized at 4 °C for 6 h. The resulting product was eluted with acetic acid / methanol (v / v=1:1) to obtain a ferulic acid-aminosilicone surface grafted molecularly imprinted polymer.

[0038] Comparative Example 3 The difference from Example 3 is that ferulic acid template molecules are not added in step 1, resulting in a non-molecularly imprinted polymer.

[0039] Under the same test conditions, the adsorption capacities of ferulic acid on the surface of the molecularly imprinted polymer and the non-imprinted polymer prepared in Example 3 and Comparative Example 3 were 124.1 mg / g and 74.0 mg / g, respectively, with an imprinting factor of 1.68.

[0040] Example 4

[0041] The difference from Example 1 is that in step 1, 30 mL of acetonitrile, 0.2 g of aminosilicone, 0.03 g of ferulic acid and 42.6 mg of acrylamide monomer were added to a 50 mL round-bottom flask, and prepolymerized at 4 °C for 6 h. The product was then eluted with acetic acid / methanol (v / v=1:1) to obtain a ferulic acid-aminosilicone surface grafted molecularly imprinted polymer.

[0042] Comparative Example 4 The difference from Example 4 is that ferulic acid template molecules are not added in step 1, resulting in a non-molecularly imprinted polymer.

[0043] Under the same test conditions, the adsorption capacities of ferulic acid on the surface of the ferulic acid-aminosilica surface-grafted molecularly imprinted polymer and the non-imprinted polymer prepared in Example 4 and Comparative Example 4 were 10.9 mg / g and 12.1 mg / g, respectively, with an imprinting factor of 0.9.

[0044] Example 5

[0045] The difference from Example 1 is that in step 1, 30 mL of acetonitrile, 0.2 g of aminosilicone, 0.03 g of ferulic acid and 41 μL of acrylic monomer were added to a 50 mL round-bottom flask and prepolymerized at 4 °C for 6 h. The resulting product was eluted with acetic acid / methanol (v / v=1:1) to obtain a ferulic acid-aminosilicone surface grafted molecularly imprinted polymer.

[0046] Comparative Example 5 The difference from Example 5 is that ferulic acid template molecules are not added in step 1, resulting in a non-molecularly imprinted polymer.

[0047] Under the same test conditions, the adsorption capacities of ferulic acid on the surface of the ferulic acid-aminosilica grafted molecularly imprinted polymer and the non-imprinted polymer prepared in Example 5 and Comparative Example 5 were 10.9 mg / g and 12.1 mg / g, respectively, with an imprinting factor of 0.84.

[0048] Figure 4 The adsorption effects of imprinted polymers prepared with different monomers in Examples 1-5 and non-imprinted polymers in Comparative Examples 1-5 are shown. The bar charts represent adsorption capacity, and the line graphs represent imprinting factors. The functional monomers are 2-vinylpyridine (2-VP), 4-vinylpyridine (4-VP), acrylamide (AM), methacrylic acid (MAA), and acrylic acid (AA). Figure 4 It can be seen that although the amount of ferulic acid adsorbed by the basic 2-VP as the functional monomer is the highest, reaching 122.5 mg / g, the non-specific adsorption is large and the actual selectivity is not as good as that of the acidic MAA. Its imprinting factor is 2.4 and the adsorption capacity is 62.4 mg / g, indicating that the imprinted polymer prepared by the acidic functional monomer has better specificity.

[0049] Example 6

[0050] The difference from Example 1 is that the crosslinking agent used in step 2 is ethylene glycol dimethacrylate, which is used to prepare ferulic acid-amino silicone surface grafted molecularly imprinted polymer.

[0051] Comparative Example 6 The difference from Example 6 is that ferulic acid template molecules are not added in step 1, resulting in a non-molecularly imprinted polymer.

[0052] Under the same test conditions, the adsorption capacities of ferulic acid on the surface of the molecularly imprinted polymer and the non-imprinted polymer prepared in Example 6 and Comparative Example 6 were 26 mg / g and 11.1 mg / g, respectively, with an imprinting factor of 2.3.

[0053] Example 7

[0054] The difference from Example 1 is that the crosslinking agent used in step 2 is trimethylolpropane triacrylate, which is used to prepare ferulic acid-amino silicone surface grafted molecularly imprinted polymer.

[0055] Comparative Example 7 The difference from Example 7 is that ferulic acid template molecules are not added in the steps to obtain a non-molecularly imprinted polymer.

[0056] Under the same test conditions, the adsorption capacities of ferulic acid on the surface of the ferulic acid-aminosilica grafted molecularly imprinted polymer and the non-imprinted polymer prepared in Example 7 and Comparative Example 7 were 29.3 mg / g and 12.4 mg / g, respectively, with an imprinting factor of 2.36.

[0057] Figure 5 The adsorption effects of imprinted polymers prepared with different crosslinking agents in Examples 1, 6, and 7, and non-imprinted polymers in Comparative Examples 1, 6, and 7 are compared. From... Figure 5 It can be seen that the imprinted polymers prepared using methyl hydroxypropane triacrylate (TMPTMA), ethylene glycol diglycidyl ether (EGDE), and ethylene glycol dimethacrylate (EGDMA) as crosslinking agents are effective. In Examples 1, 6, and 7, optimized MAA was used as the functional monomer. Although the adsorption capacity of the acrylate-based TMPTMA and EGDMA as crosslinking agents was close to that of the glycerol ether-based EGDE, the imprinting factor of the molecularly imprinted polymer prepared using EGDE as the glycerol ether crosslinking agent was significantly greater than that of the two acrylate-based crosslinking agents, with a ratio greater than 1.7. This indicates that EGDE is a more effective crosslinking agent, and the synthesized ferulic acid-aminosilicone surface-grafted molecularly imprinted polymer exhibits better specificity for ferulic acid.

[0058] The ferulic acid-aminosilica surface-grafted molecularly imprinted polymer of this invention is prepared using aminosilica as a carrier. The molecularly imprinted polymer has a particle size of 3-12 μm and an adsorption capacity of 30-50 mg / g. This imprinted polymer exhibits a fast mass transfer rate, enabling it to specifically recognize ferulic acid within a short time (1-20 min). The high specific surface area provided by the molecularly imprinted polymer grafted onto the silica core increases the number of specific binding sites in the imprinted material. The distribution of the imprinted layer on the carrier surface also makes the imprinted sites easily accessible, achieving rapid recognition of template molecules and their structural analogs. Due to the excellent specificity and selectivity of the ferulic acid-aminosilica surface-grafted molecularly imprinted polymer, it can be combined with high-performance liquid chromatography (HPLC) for the analysis and detection of ferulic acid (concentration 0.25-50 mg / g) in actual samples, enabling the identification and detection of ferulic acid in complex matrices.

[0059] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations and simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. A ferulic acid-amino silica gel surface-grafted molecularly imprinted polymer, characterized in that, The ferulic acid-aminosilica gel surface-grafted molecularly imprinted polymer is prepared by dissolving ferulic acid in a solvent, adding aminosilica gel and a monomer containing double bonds, and prepolymerizing at 0-10°C. Under a protective atmosphere, the resulting prepolymer is added to a crosslinking agent and an initiator, and polymerized at 40-90°C with stirring. The obtained product is centrifuged and eluted to remove the ferulic acid template, washed until neutral, and then vacuum dried.

2. The ferulic acid-amino silica gel surface-grafted molecularly imprinted polymer according to claim 1, characterized in that, The solvent is one or more of acetonitrile, methanol, or ethyl acetate; the double-bonded functional monomer is one or more of 2-vinylpyridine, 4-vinylpyridine, acrylamide, methacrylic acid, or acrylic acid.

3. The ferulic acid-amino silica gel surface-grafted molecularly imprinted polymer according to claim 1, characterized in that, The initiator is azobisisobutyronitrile, azobisisoheptanenitrile, azobisisovalerate, benzoyl peroxide, potassium persulfate, or ammonium persulfate; the crosslinking agent is one or more of trimethylolpropane triacrylate, ethylene glycol diglycidyl ether, or ethylene glycol dimethacrylate.

4. The ferulic acid-amino silica gel surface-grafted molecularly imprinted polymer according to claim 1, characterized in that, The molar ratio of ferulic acid to the double-bonded functional monomer is 1:(3~10); the mass ratio of aminosilicone to ferulic acid is (0.015~0.06):(0.1~0.3).

5. The ferulic acid-amino silica gel surface-grafted molecularly imprinted polymer according to claim 1, characterized in that, The mass ratio of the initiator to the crosslinking agent is (5~15):(250~300).

6. The method for preparing the ferulic acid-amino silica gel surface-grafted molecularly imprinted polymer according to any one of claims 1-5, characterized in that, The specific steps include the following: S1. Dissolve ferulic acid in a solvent, add amino silica gel and a monomer containing double bonds, and prepolymerize at 0~10℃ to form a prepolymer; S2. Under a protective atmosphere, a crosslinking agent and an initiator are added to the prepolymer, and the polymerization reaction is carried out by stirring at 40~90℃. The obtained product is centrifuged and eluted to remove the ferulic acid template, washed until neutral, and dried under vacuum to obtain a ferulic acid-aminosilicone surface-grafted molecularly imprinted polymer.

7. The method for preparing the ferulic acid-amino silica gel surface-grafted molecularly imprinted polymer according to claim 1, characterized in that, The prepolymerization reaction in step S1 takes 2 to 12 hours.

8. The method for preparing the ferulic acid-amino silica gel surface-grafted molecularly imprinted polymer according to claim 1, characterized in that, The protective atmosphere described in step S2 is helium, argon, or nitrogen.

9. The method for preparing the organic phase of the ferulic acid-amino silica gel surface-grafted molecularly imprinted polymer according to claim 1, characterized in that, The polymerization reaction in step S2 takes 6 to 24 hours.

10. The application of the ferulic acid-aminosilicone surface-grafted molecularly imprinted polymer according to any one of claims 1-5 in the field of selective recognition or detection of ferulic acid.

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