A filling assembly and control method, magnetic separation and cleaning device

Abstract

The application discloses a kind of filling assembly and control method, magnetic separation cleaning device.Adding component includes cup rotating member and injection needle, cup rotating member is used to grab reaction cup and drive it to rotate, and its central axis is provided with injection channel;Injection needle is coaxially arranged with cup rotating member and can be from injection channel into reaction cup.In control timing, cup rotating member first drives reaction cup to rotate, and after it is in rotating state, injection needle then adds cleaning fluid or substrate into reaction cup.The design will overlap the timing of filling and mixing steps, effectively reduce the filling splash and magnetic bead loss, improve the purification efficiency, and can complete efficient processing in the short residence time of magnetic separation carousel, especially suitable for high-throughput chemiluminescence immunoassay equipment, while ensuring detection accuracy significantly improve detection throughput.

Description

Technical Field

[0001] This invention belongs to the field of medical device technology, specifically relating to a dispensing component and control method, and a magnetic separation cleaning device. Background Technology

[0002] Sample analysis equipment, especially automated detection equipment based on chemiluminescence immunoassay technology, is widely used in clinical diagnostics. These devices typically integrate multiple functional units, including sample dispensing, reagent dispensing, reaction incubation, magnetic separation and cleaning, substrate dispensing, and optical signal detection. Among these, the magnetic separation and cleaning module is one of the core components determining the accuracy and repeatability of the detection results. Its function is to use a magnetic field to adsorb magnetic beads containing the target substance onto the wall of the reaction vessel, while simultaneously using a cleaning solution to remove unbound impurities, and finally adding the substrate for signal detection.

[0003] In recent years, to improve detection throughput and automation, those skilled in the art have made numerous improvements to magnetic separation cleaning devices. For example, Chinese invention patent CN104722526A discloses a magnetic separation device, including a cleaning turntable mechanism, a magnet structure, a mixing mechanism, and a cleaning needle mechanism. The cleaning turntable is equipped with multiple self-rotating components. A revolution stepper motor drives the cleaning turntable to revolve, and a self-rotation stepper motor drives the mixing disc to rotate, thereby achieving simultaneous self-rotation and mixing of cleaning liquids in multiple reaction cups. Although this invention improves washing efficiency, its mixing mechanism and cleaning needle mechanism are independent of each other, and the liquid injection and mixing are performed in steps. Furthermore, the reaction cups are stationary during liquid injection, which can easily lead to splashing of cleaning liquid and impact damage to the magnetic bead adsorption layer. Chinese invention patent CN113770104A discloses a magnetic cleaning and separation device and method for a chemiluminescence immunoassay analyzer. It includes a central rotating disk, a cleaning component, a separation component, and a mixing component. The mixing component includes a sealing mechanism and a mixing mechanism. The mixing mechanism drives the reaction cup to rotate, and the sealing mechanism blocks the opening of the reaction cup during rotation to prevent liquid splashing. This invention enhances the mixing effect by using an eccentrically connected mixing cup head to drive the reaction cup to rotate eccentrically. However, the liquid injection and mixing are still performed in steps: the cleaning needle component first injects the cleaning solution into the reaction cup, and then the reaction cup moves to the mixing position for mixing. This "inject first, then mix" sequence has the following problems in high-speed diagnostic scenarios: during injection, the reaction cup is stationary, and the cleaning solution directly impacts the magnetic bead adsorption layer, which may cause the magnetic beads to detach and be drawn away by the aspiration needle, resulting in target material loss. Simultaneously, additional mixing time is required after injection, making it difficult to complete sufficient cleaning and purification within a short residence time.

[0004] In summary, existing magnetic separation cleaning devices generally adopt the "add first, then mix" operation sequence, that is, the injection needle first adds cleaning solution to the static reaction cup, and then the cleaning solution is fully contacted with the magnetic beads by rotation or oscillation. This mode has the following technical problems in high-speed diagnostic equipment: (1) Splashing and uneven cleaning: When the cleaning solution is added to the static reaction cup, the droplets are easy to splash from the cup mouth, causing cross-contamination or reagent loss; at the same time, it is difficult for the cleaning solution to quickly and evenly rinse the inner wall of the reaction cup and the magnetic beads adsorbed on the cup wall, resulting in a decrease in cleaning and purification efficiency; (2) Risk of magnetic bead loss: When adding first and then rotating, the cleaning solution may directly impact the magnetic bead adsorption layer during the addition process, causing some magnetic beads to detach from the cup wall and be drawn away by the aspiration needle, resulting in the loss of target material and affecting detection sensitivity; (3) Timing conflict: In high-speed operation mode, adding and mixing are performed in two separate time periods, making it difficult to complete sufficient cleaning and mixing within a limited residence time, which restricts the improvement of the overall detection throughput. Therefore, there is an urgent need to provide a sample analysis device that can achieve efficient and low-loss cleaning and substrate loading by optimizing the loading sequence through structural improvements without significantly increasing structural complexity. Summary of the Invention

[0005] To address the aforementioned problems, this invention provides a dispensing component and control method, as well as a magnetic separation and cleaning device. By structurally improving the dispensing component, the injection needle and the gripping cup rotating component are designed as a coaxial integrated unit, and the control timing is optimized. The gripping cup rotating component first clamps the reaction cup and drives it to rotate. After the reaction cup is rotating, the cleaning fluid or substrate is added into it by the coaxial injection needle. This design overlaps the mixing and dispensing steps in sequence, effectively solving the problems of dispensing splash and uneven cleaning. While improving the cleaning and purification efficiency and reducing the magnetic bead loss rate, it significantly compresses the single operation cycle, making it particularly suitable for high-speed diagnostic scenarios. Thus, while ensuring the accuracy of the test results, it greatly improves the overall detection throughput.

[0006] This invention provides a dispensing component, comprising: A cup-gripping rotator is used to grip the reaction cup and drive the reaction cup to rotate around its own axis. A liquid injection channel is provided at the central axis of the cup-gripping rotator. The injection needle is coaxially arranged with the rotating gripper cup. The injection needle can extend into the injection channel and act on the reaction cup. The injection needle is used to inject cleaning fluid into the reaction cup.

[0007] By adopting the above-mentioned technical solution, the dispensing component in this application, through the setting of a gripping cup rotating component and a liquid injection needle coaxial with the gripping cup rotating component, allows the gripping cup rotating component to first grip the reaction cup and drive it to rotate during the dispensing of cleaning fluid, and then the liquid injection needle to dispensing cleaning fluid into the rotating reaction cup. This dispensing component optimizes and integrates the rotational movement of the reaction cup and the dispensing operation of the liquid injection needle in a timing sequence, so that the cleaning fluid can form a rotating flow vortex in the reaction cup, thereby effectively reducing the risk of liquid splashing and cross-contamination during the dispensing process. At the same time, the rotating reaction cup allows the cleaning fluid to more evenly wash the magnetic beads on the cup wall, improving the cleaning and purification efficiency and providing a structural basis for the accuracy of subsequent detection results.

[0008] Furthermore, the cup-gripping rotating component includes a connecting end and multiple grippers. Multiple grippers are spaced apart at the connecting end and extend downward. The inner walls of the multiple grippers together form a working area for holding the reaction cup. The liquid injection channel is formed at the central axis of the connecting end and the multiple grippers. The axis of the injection needle coincides with the axis of the cup-gripping rotating component. When the cup-gripping rotating component clamps and drives the reaction cup to rotate, the injection needle injects cleaning fluid into the rotating reaction cup through the injection channel.

[0009] Thanks to the aforementioned technical solution, the rotating cup gripper forms a central injection channel through the cooperation of its connecting end and multiple jaws, ensuring that the injection needle can extend into the reaction cup along the central axis of the rotating cup gripper. The working area formed by the inner walls of the multiple jaws stably grips the reaction cup, ensuring that the reaction cup and the injection needle remain coaxial while the rotating cup gripper drives the reaction cup to rotate. This structure allows the injection needle to deliver cleaning fluid into the reaction cup undisturbed through the injection channel during high-speed rotation of the reaction cup, ensuring both the accuracy of cleaning fluid injection and avoiding mechanical failures caused by structural interference, thereby guaranteeing the accuracy of cleaning fluid injection and the stability of rotational mixing.

[0010] Furthermore, it also includes: The gripper push rod is coaxially arranged with the cup-gripping rotating component and is movably inserted into the liquid injection channel; The bottom of the gripper push rod is provided with a push head; In its natural state, the radial dimension of the working area formed by the multiple grippers gradually decreases from top to bottom; When the gripper push rod is driven to move downward, the push head extends into the working area, and the outer peripheral surface of the push head pushes the inner wall of the multiple grippers radially outward. As the insertion depth of the push head increases, the grippers continuously undergo elastic deformation and open radially outward, thereby expanding the bottom opening of the working area to allow the top of the reaction cup to enter the working area. When the gripper push rod is driven to move upward, the push head retracts from the action area, and the multiple grippers retract radially inward under their own elastic restoring force, and clamp the reaction cup by abutting the top outer wall of the reaction cup through the bottom end of the gripper; when the gripper clamps the reaction cup, the bottom end of the gripper is in an elastic deformation state, and at this time the opening of the bottom end of the gripper is greater than its opening in the natural state.

[0011] Thanks to the aforementioned technical solution, the coaxial connection between the gripper push rod and the rotating cup-gripping component, combined with the pushing action of the pusher head against the inner wall of the gripper, achieves mechanical gripping and release of the reaction cup. When the gripper push rod moves downward, the pusher head forces the gripper to elastically open to receive the top of the reaction cup; when the gripper push rod moves upward, the gripper retracts inward and clamps the reaction cup using its own elastic restoring force. This purely mechanical clamping method is simple in structure, reliable in operation, and the clamping force comes from the elastic deformation of the gripper itself, eliminating the need for additional pneumatic or electric drive sources. This reduces the complexity of the components and manufacturing costs while ensuring stable clamping of the reaction cup during high-speed rotation, avoiding the risk of the reaction cup falling off under centrifugal force. When the gripper clamps the reaction cup, the bottom end of the gripper remains in an elastic deformation state, and its opening is greater than its natural opening, thus ensuring a continuous and reliable clamping preload on the reaction cup.

[0012] Furthermore, the gripper push rod moves independently up and down relative to the cup-gripping rotating component to drive the multiple grippers to open or close; The injection needle moves up and down independently relative to the gripper push rod and the cup rotating component. The injection needle descends and extends into the reaction cup to inject cleaning fluid.

[0013] By adopting the above technical solution, this application achieves independent control and timing optimization of the three actions of cup gripping, rotation, and filling by setting the gripper push rod to rise and fall independently relative to the cup-gripping rotating component, and the injection needle to rise and fall independently relative to both the gripper push rod and the cup-gripping rotating component. Only after the reaction cup is gripped and reaches a preset time or preset rotation speed does the injection needle independently descend and insert into the reaction cup to inject the cleaning fluid. This avoids direct impact of the cleaning fluid on the magnetic bead adsorption layer in a stationary state during injection, while ensuring the relative position stability of the injection needle and the rotating reaction cup during injection. This independent lifting structure ensures that the movements of each functional component do not interfere with each other, improving the reliability of the actions and the flexibility of control, and providing a structural basis for achieving the "rotate first, then inject" timing sequence.

[0014] Furthermore, it also includes a filling rotary drive mechanism, which includes a filling rotary drive motor, multiple filling rotary drive wheels, and a filling rotary transmission belt; Multiple filling rotary drive wheels are connected to the connecting end of the cup gripping rotating component and can drive the cup gripping rotating component to rotate synchronously; the axial center of the multiple filling rotary drive wheels is provided with a liquid injection channel, and the gripper push rod passes through the liquid injection channel and acts on the gripper; The filling rotary transmission belt is wound around and connected to multiple filling rotary drive wheels in a roundabout and zigzag manner, so as to drive the multiple filling rotary drive wheels to rotate synchronously; The filling rotary drive motor drives multiple filling rotary drive wheels to rotate via a filling rotary transmission belt, thereby causing the cup gripping rotating component to rotate synchronously with the clamped reaction cup.

[0015] By adopting the above technical solution, this application achieves efficient and stable driving of the cup-gripping rotating component by setting up a transmission mechanism consisting of a filling rotary drive motor, multiple filling rotary drive wheels, and a filling rotary transmission belt. The filling rotary drive wheels are connected to the connecting end of the cup-gripping rotating component, and the filling rotary drive motor drives the filling rotary drive wheels and the cup-gripping rotating component to rotate via the filling rotary transmission belt. The filling rotary transmission belt winds around multiple filling rotary drive wheels in a meandering and reversing manner, which can simultaneously drive multiple cup-gripping rotating components to rotate synchronously, improving driving efficiency and simplifying the transmission structure. This belt drive has a certain buffering and shock absorption effect, which can effectively reduce the impact of vibration on the stability of the liquid inside the reaction cup during rotation, providing a stable operating environment for the injection needle to accurately fill the rotating reaction cup.

[0016] A magnetic separation cleaning device includes a device body and an operating unit. The operating unit is located above the device body and is used to perform cleaning liquid filling, waste liquid suction and displacement, and reaction cup clamping and rotation operations on the reaction cup inside the device body. The operating unit includes the above-mentioned filling component and also includes a liquid suction needle connecting plate, a push rod connecting plate, and a filling connecting plate arranged sequentially along the height direction. The suction needle connecting base plate is used to mount the suction needle and drive the suction needle to move up and down independently; A gripper push rod is mounted on the push rod connecting base plate. The gripper push rod is coaxially arranged with the dispensing assembly and can be inserted into the dispensing assembly and act on the gripper of the cup-gripping rotating part. The refilling connection base plate is used to install the refilling assembly and drive the refilling assembly to rise and fall as a whole.

[0017] By adopting the above-mentioned technical solution, the magnetic separation cleaning device of this application integrates the filling component into the operating unit, and sets up a filling connection base plate, a push rod connection base plate, and a suction needle connection base plate for mounting the filling component, the gripper push rod, and the suction needle, respectively. Each base plate is arranged sequentially along the height direction and can move independently or in conjunction, making the entire operating unit compact and hierarchically structured, achieving a modular layout of multiple functional units within the magnetic separation cleaning device. This modular design not only facilitates assembly and maintenance but also allows each functional unit to operate independently according to control commands, improving the flexibility and operating efficiency of the magnetic separation cleaning device in multi-station, multi-step cleaning processes.

[0018] Furthermore, the injection connection base plate, the push rod connection base plate, and the suction needle connection base plate are all located above the device body and can be raised and lowered independently relative to the device body; The filling connection base plate is configured to be driven to cause the entire filling assembly to move independently up and down relative to the lower device body; The push rod connecting base plate is movably connected to the filling connecting base plate so that the push rod connecting base plate can move up and down synchronously with the filling connecting base plate relative to the device body, and the push rod connecting base plate can also be driven to move up and down independently relative to the filling connecting base plate, thereby driving the gripper push rod to move up and down independently in the liquid injection channel. The suction needle connecting base plate is configured to be driven to move independently relative to the device body.

[0019] By adopting the above-mentioned technical solution, this application allows the filling connecting base plate, push rod connecting base plate, and suction needle connecting base plate to be independently raised and lowered relative to the device body. The filling connecting base plate drives the overall raising and lowering of the filling assembly, while the push rod connecting base plate can both be raised and lowered synchronously with the filling connecting base plate and be driven to raise and lower independently relative to the filling connecting base plate. This causes the gripper push rod to raise and lower independently within the liquid injection channel, and the suction needle connecting base plate can also be raised and lowered independently. This multi-layer base plate configuration of independent and linked raising and lowering ensures that the vertical movements of the filling assembly, gripper push rod, and suction needle are coordinated yet do not interfere with each other. It can accurately adapt to the different height requirements of different stations in the magnetic separation cleaning device, realizing the parallel and orderly operation of multiple actuators within a compact space, further improving the overall operating efficiency and operational reliability of the device.

[0020] Furthermore, the device body includes: A magnetic separation disk includes a magnetic separation turntable that can rotate around an axis, and an inner disk and an outer disk that are fixedly disposed on the inner and outer sides of the magnetic separation turntable and are coaxial with the magnetic separation turntable; the magnetic separation turntable is provided with a plurality of receiving positions in the circumferential direction for receiving and driving the reaction cup to rotate. A plurality of configuration slots are spaced apart along the circumferential direction on the inner disk and the outer disk; A magnetic component is disposed within the configuration slot; The magnetic separation disk has several stations along its circumference. Each station is one of a dual-pole station, a single-pole station, or a non-magnetic-pole station. At the dual-pole station, both the inner and outer disks are provided with configuration slots, and the magnetic components in the configuration slots have the same magnetic polarity. At the single-pole station, either the inner or outer disk is provided with a configuration slot. At the non-magnetic-pole station, neither the inner nor outer disks are provided with configuration slots.

[0021] By adopting the above-mentioned technical solution, the device body of this application adopts a structure with a fixed inner and outer disk and a rotating magnetic separation turntable. Configuration slots and magnetic components are spaced along the circumference on the inner and outer disks, forming multiple magnetic field distribution modes: dual-pole, single-pole, and poleless. In the dual-pole position, magnetic components of the same polarity on the inner and outer disks apply a unidirectional magnetic field force to the magnetic beads in the reaction cup, causing the magnetic beads to be dispersed and adsorbed near the cup walls of the inner and outer disks. The central area of ​​the reaction cup becomes a magnetically unstable region, thus minimizing the loss of magnetic beads during liquid aspiration. In the single-pole position, a unilateral magnetic field causes the magnetic beads to be concentrated and adsorbed on one side of the cup wall, facilitating cleaning or substrate addition in specific steps. Through the differentiated arrangement of magnetic components at different positions, the magnetic separation cleaning device can precisely control the adsorption position and state of the magnetic beads in the reaction cup, improving the efficiency of magnetic separation cleaning and the retention rate of the target substance.

[0022] A control method for a dispensing component, applicable to the aforementioned dispensing component, the method comprising the following steps: Grabbing steps: Control the entire filling component to descend and approach the reaction cup, and use the cup-grabbing rotating part to grab the reaction cup; Rotation step: Drive the gripping cup rotating component to rotate around its own axis, thereby causing the clamped reaction cup to rotate synchronously, so that the liquid contained in the reaction cup is driven into the rotation dynamic. Addition steps: While the reaction cup is in a rotating state, control the injection needle to extend downward into the reaction cup along the injection channel, and add cleaning fluid into the rotating reaction cup; Reset Procedure: After filling is complete, stop the injection and control the injection needle to reset. Control the cup gripper to stop rotating and use the cup gripper to return the reaction cup to the target position.

[0023] By employing the aforementioned technical solution, the control method of this application, based on the structural features of the aforementioned dispensing component, achieves the operational sequence of first clamping and rotating the reaction cup, and then dispensing the cleaning solution while the cup is rotating, by sequentially executing the gripping step, rotation step, dispensing step, and reset step. Dispensing the cleaning solution while the reaction cup is in a rotating dynamic state allows the cleaning solution to form a rotating flow vortex within the reaction cup, effectively reducing the risk of liquid splashing and cross-contamination during the dispensing process. Simultaneously, the rotating reaction cup allows the cleaning solution to more evenly wash the magnetic beads on the cup wall, improving cleaning and purification efficiency. This control method overlaps the mixing and dispensing steps in a specific time sequence, significantly compressing the single operation cycle, making it particularly suitable for high-speed diagnostic scenarios, thereby greatly increasing the overall detection throughput while ensuring the accuracy of the test results.

[0024] Furthermore, the cup-gripping rotating component includes a connecting end and multiple grippers. The multiple grippers are spaced apart at the connecting end and extend downward. The inner walls of the multiple grippers together form an area for gripping the reaction cup. The injection channel is formed at the central axis of the connecting end and the multiple grippers. The filling assembly also includes a gripper push rod. The gripper push rod is coaxially arranged with the cup-gripping rotating component and is movably inserted into the injection channel. The bottom of the gripper push rod is provided with a push head. In its natural state, the radial dimension of the area formed by the multiple grippers gradually decreases from top to bottom. During the gripping step, the gripper push rod is driven to move downward, causing the pusher head to extend into the working area and push the inner walls of the multiple grippers radially outward, causing the grippers to elastically deform and open radially outward, allowing the top of the reaction cup to enter the working area; the gripper push rod is then driven to move upward, causing the pusher head to withdraw from the working area, and the multiple grippers retract radially inward under their own elastic restoring force, clamping the reaction cup by abutting the top outer wall of the reaction cup with the bottom of the grippers; In the reset step, the gripper rotating component is controlled to move the reaction cup above the target position, and the gripper push rod is driven to move downward relative to the gripper rotating component, so that the push head extends into the working area again and pushes the inner wall of the multiple grippers radially outward, causing the grippers to elastically deform and open radially outward, thus expanding the opening at the bottom of the working area. The bottom end of the gripper disengages from the top outer wall of the reaction cup, thereby releasing the reaction cup and placing it back to the target position. Then, the gripper push rod is driven to move upward, so that the push head withdraws from the working area, and the multiple grippers retract radially inward under their own elastic restoring force to return to their natural state. The entire dispensing assembly is then controlled to rise and reset.

[0025] By employing the aforementioned technical solution, the control method of this application achieves precise gripping and release of the reaction cup in the gripping and resetting steps through the downward and upward movement of the gripper push rod, combined with the pushing action of the pusher head against the inner wall of the gripper and the elastic restoring force of the gripper itself. In the gripping step, the gripper push rod first moves downward to open the gripper head to receive the top of the reaction cup, then moves upward to retract the pusher head. The gripper, relying on its elastic restoring force, contracts and clamps the reaction cup. During clamping, the bottom end of the gripper remains in an elastic deformation state to provide continuous clamping force. In the resetting step, the gripper push rod moves downward again to open the gripper head, disengaging the bottom end of the gripper from the reaction cup, thus returning the reaction cup to the target position. Then, the gripper push rod moves upward, and the gripper returns to its natural state. This mechanical gripping and releasing control method is simple in structure and reliable in operation, requiring no additional pneumatic or electric drive source. It ensures stable gripping and accurate release of the reaction cup even during high-speed rotation and repeated operations, further improving the automation control accuracy and operational reliability of the dispensing assembly.

[0026] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are: 1. This invention integrates a cup-gripping rotating component and a liquid injection needle coaxially. The cup-gripping rotating component has a liquid injection channel at its center, and the liquid injection needle extends into the reaction cup through the channel, realizing rotation before injection, reducing splashing and contamination, improving cleaning efficiency and reducing magnetic bead loss.

[0027] 2. The cup-gripping rotating component of the present invention includes a connecting end and multiple grippers. The central axis forms an injection channel. The injection needle is coaxial with the cup-gripping rotating component. The rotation and injection are coaxially overlapped to ensure stable clamping and avoid eccentric vibration and injection offset.

[0028] 3. The clamping push rod of the present invention is coaxially inserted through the liquid injection channel. The push head pushes the inner wall of the clamping claw to open the clamping claw. After withdrawal, the clamping claw elastically contracts to clamp the reaction cup. When clamping, the bottom end maintains elastic deformation to ensure continuous and stable clamping without damaging the reaction cup.

[0029] 4. The gripper push rod, injection needle and cup rotating component of the present invention are decoupled in motion and rise and fall independently. The opening of the gripper and the height of the injection needle can be flexibly adjusted, which improves adaptability and operational flexibility and avoids motion interference and mechanical failure.

[0030] 5. The filling rotary transmission belt of the present invention is wound around multiple filling rotary drive wheels in a meandering and reversing path, realizing the synchronous drive of multiple cup-gripping rotating parts by a single motor, and ensuring the consistency and stability of the rotation speed of the reaction cups at each station.

[0031] 6. The filling connection base plate, push rod connection base plate, and suction needle connection base plate of the present invention can be raised and lowered independently relative to the device body, and the push rod connection base plate can move independently as well as follow, realizing layered independent driving and improving operational accuracy and control flexibility.

[0032] 7. The magnetic separation disk of the present invention is provided with an inner disk, an outer disk and a magnetic separation turntable. Magnetic components are arranged in the groove to form a double magnetic pole, a single magnetic pole and a non-magnetic pole station. The alternating magnetic attraction promotes the contact between the magnetic beads and the cleaning fluid, improves the cleaning efficiency and meets the high throughput requirements.

[0033] 8. The dispensing component control method of the present invention includes cup gripping, rotation, dispensing and resetting steps, realizing rotation before dispensing, avoiding splashing and magnetic bead impact during static dispensing, reducing target loss and improving detection sensitivity. Attached Figure Description

[0034] Figure 1 This is a liquid circuit connection diagram of the injection needle and suction needle corresponding to a magnetic separation cleaning device provided in an embodiment of the present invention; Figure 2 This is a liquid circuit connection diagram of the substrate needle corresponding to a magnetic separation cleaning device provided in an embodiment of the present invention; Figure 3 This is a schematic diagram of the magnetic body arrangement structure of the functional operating station of a magnetic separation and cleaning device provided in an embodiment of the present invention; Figure 4 This is a top view of the magnetic structure of the magnetic body of a magnetic separation and cleaning device provided in an embodiment of the present invention, in which all magnetic poles are arranged in an N-pole configuration. Figure 5 This is a top view of the magnetic body of a magnetic separation and cleaning device provided in an embodiment of the present invention, showing that all the magnetic elements of the device are arranged with S poles. Figure 6 This is a schematic diagram of the overall structure of a magnetic separation cleaning device provided in an embodiment of the present invention; Figure 7 This is a schematic diagram of the layout of the liquid injection station of the magnetic separation turntable in a magnetic separation cleaning device provided in an embodiment of the present invention; Figure 8 This is a schematic diagram of the magnetic separation turntable magnetic suction station layout of a magnetic separation cleaning device provided in an embodiment of the present invention; Figure 9 This is a schematic diagram of the magnetic separation turntable liquid transfer station layout of a magnetic separation cleaning device provided in an embodiment of the present invention; Figure 10 (a) is a schematic diagram of the filling component of a magnetic separation cleaning device provided in an embodiment of the present invention for preparing the cleaning fluid; Figure 10 (b) is a schematic diagram of the filling component holding the reaction cup in a magnetic separation cleaning device provided in an embodiment of the present invention; Figure 10 (c) is a diagram illustrating the process of adding cleaning fluid to the filling component of a magnetic separation cleaning device provided in an embodiment of the present invention; Figure 11 (a) is a schematic diagram of the reaction cup being rotated by the gripping cup rotating component of a magnetic separation cleaning device provided in an embodiment of the present invention; Figure 11 (b) is a schematic diagram of the injection needle of a magnetic separation cleaning device provided in an embodiment of the present invention injecting cleaning fluid into a rotating reaction cup; Figure 12 This is a flowchart illustrating the purification and substrate addition process performed in the reaction vessel of a magnetic separation and cleaning apparatus provided in an embodiment of the present invention. Figure 13 This is a three-dimensional structural diagram of a sample analysis device provided in an embodiment of the present invention; Figure 14 This is a top view of a sample analysis device provided in an embodiment of the present invention; Figure 15 This is a schematic diagram of the structure of a rotating mixing mechanism of a sample analysis device provided in an embodiment of the present invention; Figure 16 This is an execution detection state diagram of a signal detection device for a sample analysis equipment provided in an embodiment of the present invention.

[0035] Explanation of reference numerals in the attached figures: 10-Reagent carrier; 11-Reaction incubation device; 12-Magnetic separation and cleaning device; 13-Signal detection device; 16-Rotating mixing mechanism; 17-Tube rack transfer mechanism; 100-Reaction cup; 1011-Reagent rack receiving slot; 1200-Cup gripping rotating component; 1201-First-order aspiration needle; 1202-Second-order aspiration needle; 1203-Third-order aspiration needle; 1204-Fourth-order aspiration needle; 121-Aspiration needle connecting base plate; 1211-First-order injection needle; 1212-Second-order injection needle; 1213-Third-order injection needle; 1214-Fourth-order injection needle; 12111-Aspiration lifting drive motor; 12112-Aspiration lifting transmission screw; 1215-Substrate needle; 122-Addition connecting base plate ; 123-Push rod connecting base plate; 1230-Magnetic separation turntable; 1231-First-stage liquid injection station; 1232-Second-stage liquid injection station; 1233-Third-stage liquid injection station; 1234-Fourth-stage liquid injection station; 1235-Substrate dispensing station; 124-Operating part mounting base plate; 1241-First-stage pipetting station; 1242-Second-stage pipetting station; 1243-Third-stage pipetting station; 1244-Fourth-stage pipetting station; 1251-First magnetic suction position; 1252-Second magnetic suction position; 1253-Third magnetic suction position; 1254-Fourth magnetic suction position; 1255-Fifth magnetic suction position; 1256-Sixth magnetic suction position; 1260-Gripper push rod; 1261-Push rod lifting transmission screw; 1262-Push rod lifting... 12601-Drive motor for lowering; 12701-Push head; 12701-Washing head rotation drive motor; 12702-Transmission drive wheel; 12703-Washing head rotation transmission belt; 12704-Washing head rotation drive wheel; 12705-Tension wheel; 130-Optical signal acquisition unit; 1301-Reading position; 131-Reading hole; 1321-Reading rotation drive motor; 1322-Reading rotation drive wheel; 1323-Reading rotation transmission belt; 141-Reaction cup storage unit; 142-Reaction cup lifting mechanism; 143-Reaction cup transfer unit; 144-Cup handling turntable; 151-Sample tube rack buffer unit; 152-Sample tube rack transfer unit; 160-Cup rotation position; 1601-Rotation motor; 1602-Rotation drive wheel; 1603- Rotary drive belt; 1604-Rotary driven wheel; 1605-Rotary shaft; 1606-Sensing block; 1607-Rotary sensor; 201-Reagent dispensing probe; 202-Sample dispensing probe; 203-Waste liquid suction and transfer mechanism; 2031-Waste suction lifting drive motor; 2032-Waste suction drive wheel; 2033-Waste suction drive belt; 2034-Waste suction drive wheel; 211-First transfer mechanism; 212-Second transfer mechanism; C10-Cleaning section; M11-Inner magnetic component; M12-Outer magnetic component; P10-Peristaltic pump assembly; P20-Plug rod driven pump assembly; P211-Substrate plunger pump; S101-First substrate storage tank; S102-Second substrate storage tank; V10-Three-way valve assembly; W10-Waste liquid recovery section. Detailed Implementation

[0036] The present invention will now be described in detail with reference to the accompanying drawings.

[0037] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0038] Example 1 Figure 1 This is a liquid circuit connection diagram of the injection needle and suction needle corresponding to the magnetic separation cleaning device provided by the present invention. Figure 2 This is a liquid circuit connection diagram of the substrate needle corresponding to the magnetic separation and cleaning device provided by the present invention. The reaction system liquid formed by mixing biological samples and reagents is subjected to isothermal incubation reaction. In order to obtain accurate quantitative results of target substances, magnetic separation and cleaning and substrate addition are required. Finally, the final detection result can be obtained after detection.

[0039] like Figure 1 As shown, the present invention has multiple reaction cup receiving positions arranged in the circumferential direction of the magnetic separation turntable 1230, each of which can receive reaction cup 100. A fixed inner and outer disk are coaxially arranged on the inner and outer sides of the magnetic separation turntable 1230, respectively. Configuration grooves are provided in a portion of the circumferential direction of the inner and outer disks. Magnetic components are placed in at least a portion of the configuration grooves to form different operating positions that cooperate with the magnetic separation turntable 1230. Here, the magnetic separation cleaning device 12 has four injection stations, and correspondingly, four transfer stations. The first-order injection needle 1211 is fluidly connected to one of the piston rod drive pumps in the piston rod drive pump group P20, and finally connected to the cleaning section C10 through pipes and valves. The cleaning section C10 can have at least one cleaning tank arranged side-by-side, thus achieving… For a more continuous supply of cleaning fluid, the second-stage injection needle 1212 is fluidly connected to another piston rod driven pump within the piston rod driven pump assembly P20, and ultimately connected to the cleaning section C10 via pipes and valves. Similarly, the third-stage injection needle 1213 and the fourth-stage injection needle 1214 have the same connection structure. A three-way valve assembly V10 is also configured between the piston rod driven pump assembly P20 and the aforementioned first-stage injection needle 1211, second-stage injection needle 1212, third-stage injection needle 1213, and fourth-stage injection needle 1214. This allows the four different injection needles to converge and connect to the same cleaning section C10. The suction needles corresponding to the first-stage injection needle 1211, second-stage injection needle 1212, third-stage injection needle 1213, and fourth-stage injection needle 1214 are connected as follows: Figure 1As shown, the first-order suction needle 1201 is connected to one of the peristaltic pumps in the peristaltic pump group P10. Preferably, the peristaltic pump is used as the suction force driving the suction needle, which can avoid problems such as air blockage and achieve more complete transfer of liquid in the reaction cup 100. The peristaltic pump is also connected to the waste liquid recovery section W10, which is equipped with at least one waste liquid storage tank. Here, the first-order suction needle 1201 can be inserted into the reaction cup 100 from the central axis of the reaction cup 100 to suction liquid. At the first-order liquid transfer station, a first magnetic element is arranged in the configuration slots of the inner and outer disks. The first magnetic element located in the inner disk is denoted as the inner magnetic element M11, and the first magnetic element located in the outer disk is denoted as the outer magnetic element M12. The inner magnetic element M11 and the outer magnetic element M12 have the same magnetic and magnetic field strength, and here... The inner magnetic component M11 and the outer magnetic component M12 can be designed with their N poles facing each other. The N poles of both magnetic components are arranged close to the wall of the reaction cup 100. The influence of the same magnetism and the same magnetic field strength makes the central region of the reaction cup 100 a magnetically unstable region. The magnetic beads in this region cannot exist stably. Under the action of the magnetic components with the same magnetic field strength, the magnetic beads will be quickly dispersed and adsorbed near the inner or outer disk wall. In this way, when the first-order aspiration needle 1201 is used for liquid transfer, it can be ensured that the magnetic beads inside the reaction cup 100 are basically not lost. The second-order aspiration needle 1202 is connected to another peristaltic pump in the peristaltic pump group P10. This peristaltic pump is connected to the same waste liquid recovery section W10. Similarly, the third-order aspiration needle 1203 and the fourth-order aspiration needle 1204 also have the same connection structure.

[0040] Preferably, in one case, the peristaltic pump unit P10 can be a four-channel peristaltic pump driven by a single motor, making the overall structural design more compact and reasonable.

[0041] like Figure 2 As shown, the magnetic separation cleaning device 12 also includes a substrate filling station 1235. The substrate needle 1215 is fluidly connected to the substrate storage section. To ensure that the equipment is suitable for batch continuous operation, the substrate storage section includes two connection points for installing the first substrate storage tank S101 and the second substrate storage tank S102, respectively. The two substrate storage tanks are fluidly connected to the substrate needle 1215 through a two-position three-way valve. In order to accurately fill the substrate, a substrate plunger pump P211 is also configured between the substrate needle 1215 and the substrate storage tank three-way valve. The substrate plunger pump P211 can be driven independently to ensure accurate and efficient substrate filling. A drive three-way valve is also connected between the substrate plunger pump P211 and the substrate storage tank three-way valve, so that the substrate can be drawn from one of the substrate storage tanks and filled into the reaction cup 100.

[0042] like Figures 3-5 It can be seen that the magnetic separation turntable 1230 includes multiple reaction cup receiving positions, which can receive the transferred reaction cups 100 and drive the reaction cups 100 to rotate. Figures 3-5The diagram illustrates that the magnetic separation turntable 1230 can drive the reaction cup 100 to rotate clockwise. The reaction cup 100 is first rotated to a position that aligns with the first-stage injection station 1231 of the magnetic separation cleaning device 12. At this position, the first-stage injection needle 1211 can initially inject a preset amount of cleaning solution into the reaction cup 100. Since the reaction cup 100 still contains the incubated reaction system liquid, the amount of cleaning solution injected into the first-stage injection station 1231 is small, sufficient for initial dilution and cleaning. Neither the inner nor outer disk of the first-stage injection station 1231 is equipped with magnetic components; only simple cleaning solution injection is required here. Then, the magnetic separation turntable 1230 drives the reaction cup 100 to the first magnetic attraction position 1251. At position 1251, the inner and outer disks are provided with configuration slots, each containing a third magnetic element. The two separately arranged third magnetic elements have the same magnetic and magnetic field strength. Under their influence, the magnetic beads inside the reaction cup 100 cause the target material to adhere separately to the cup wall near the magnetic element, allowing the target material to be initially agglomerated and adsorbed. This separate adsorption allows for more thorough cleaning of the target material. The magnetic separation turntable 1230 continues to rotate, moving the reaction cup 100 to the second magnetic attraction position 1252. Here, the inner and outer disks of the second magnetic attraction position 1252 are provided with configuration slots, each containing a second magnetic element. The two separately arranged second magnetic elements have the same magnetic and magnetic field strength. Preferably, the magnetic field strength of the second magnetic element is less than that of the third magnetic element. The magnetic field strength of the two magnetic elements is preferably set to a ratio between 0.78 and 0.95. This allows the magnetic fields of the two magnetic attraction positions to change, resulting in a change in the adsorption position and area of ​​the magnetic beads within the reaction cup 100. This enables the magnetic beads to drive the target material with relatively large relative motion, improving cleaning efficiency, reducing the existence of magnetic dead zones, and preventing excessive aggregation of the magnetic beads during magnetic attraction. The magnetic beads in the second magnetic attraction position 1252 can both drive the target material to move sufficiently, ensuring cleaning efficiency, and maintain a moderate magnetic attraction. The magnetic separation turntable 1230 continues to rotate, moving the reaction cup 100 to the third magnetic attraction position 1253. At the third magnetic attraction position 1253, a third magnetic element is arranged in the configuration slots of the inner and outer disks. Within the suction position, the magnetic beads inside the reaction cup 100 undergo alternating magnetic attraction—strong magnetic attraction, weak magnetic attraction, and strong magnetic attraction. Preferably, the magnetic separation turntable 1230 drives the reaction cup 100 to move with intermittent rotational motion, interspersed with 6-11 seconds of stillness between rotational intervals. This ensures sufficient magnetic attraction through three magnetic attraction processes. Simultaneously, the arrangement of magnetic components on both sides strengthens the magnetic field. Compared to existing technologies requiring longer dwell times, the magnetic component arrangement of this invention enables faster magnetic adsorption. In schemes with magnetic components arranged on one side, a magnetic dwell time of 15-20 seconds is typically needed to ensure sufficient adsorption. This invention is more suitable for high-speed diagnostic scenarios.As the magnetic separation turntable 1230 continues to rotate, the reaction cup 100 is positioned within the first-order pipetting station 1241. Both the inner and outer disks of the first-order pipetting station 1241 contain mounting slots, each containing a first magnetic element. The first magnetic element located on the inner disk is designated as the inner magnetic element M11, and the first magnetic element located on the outer disk is designated as the outer magnetic element M12. The inner magnetic element M11 and the outer magnetic element M12 have the same magnetic and magnetic field strength. Preferably, the magnetic field strength of the first magnetic element is the same as that of the third magnetic element. The first-order suction needle 1201 included in the magnetic separation cleaning device 12 can be inserted into the reaction cup 100 near its central axis to apply the liquid. The reaction cup 100 is attracted and moved, and then, as the magnetic separation turntable 1230 continues to rotate, it is positioned at the second-stage injection station 1232. Here, the second-stage injection station 1232 only has a fourth magnetic component arranged in the configuration slot of the outer disk. The fourth magnetic component has the same magnetism as the first magnetic component, but its magnetic field strength is less than that of the first magnetic component. Preferably, to ensure the versatility of components within the module, the fourth magnetic component has the same magnetic field strength as the second magnetic component. Afterward, the reaction cup 100 is positioned at the same three-stage magnetic attraction position as before. Then, the cleaning liquid is transferred using the second-stage suction needle 1202 at the second-stage pipetting station 1242. The reaction cup 100 continues to rotate and is positioned at the third-stage injection station 1233. Subsequently... After three magnetic attraction cycles, the reaction cup 100 rotates to the third-stage pipetting station 1243, where the third-stage suction needle 1203 aspirates the cleaning solution. Preferably, the pipetting stations also include at least one magnetic attraction position. The magnetic separation disk rotates to align the reaction cup 100 with the fourth-stage injection station 1234. The magnetic component configuration of the fourth-stage injection station 1234 differs from that of the previous injection stations. Here, both the inner and outer disks are equipped with fourth magnetic components, making the final magnetic attraction stronger and achieving a better auxiliary mixing effect. The magnetic separation disk 1230 continues to rotate, aligning the reaction cup 100 with the fourth magnetic attraction position 1254, the fifth magnetic attraction position 1255, and the sixth magnetic attraction position 1256. Preferably, this is... To facilitate the adsorption of magnetic beads closer to the bottom of the cup, the heights of the magnetic components at the three magnetic adsorption positions are arranged in descending order. After completion, the reaction cup 100 is placed in the IV-stage pipetting station 1244, where the IV-stage suction needle 1204 is used to aspirate and transfer cleaning fluid to complete the cleaning operation within the reaction cup 100. Then, the reaction cup 100 is placed in the substrate filling station 1235, and the substrate liquid is added into the reaction cup 100 using the substrate needle 1215. The substrate filling station 1235 is equipped with magnetic components arranged radially spaced from the reaction cup 100 along the turntable. Preferably, the radial distance between the magnetic components in the substrate filling station 1235 and the reaction cup 100 is greater than the radial distance between the magnetic components and the reaction cup 100 at other stations.The spacing between the reaction cups is within a range of 2 to 10 times their original size. At the substrate filling station 1235, the gripper holds the reaction cup 100 and raises it by a preset distance so that the magnetic field influence area of ​​the magnetic component can cover at least a portion of the bottom area of ​​the reaction cup 100. After being raised, the reaction cup 100 can be rotated. Here, the magnetic field influence of the magnetic component is used to ensure that the added substrate is mixed more evenly and quickly. After the substrate filling is completed, the magnetic separation and cleaning device 12 can output the reaction cup 100.

[0043] Depend on Figures 6-9It is understood that the magnetic separation cleaning device 12 includes a device body at the bottom and an operating part at the top. The device body includes a magnetic separation turntable 1230 that can be driven to rotate. The magnetic separation turntable 1230 is arranged in an annular space between the stationary inner and outer discs in the magnetic separation disk. The magnetic separation turntable 1230 has multiple spaced receiving positions arranged in the circumferential direction, with each pair of receiving positions having a substantially equal spacing distance. Preferably, the outer disc can be wrapped with a heat insulation layer to ensure that the magnetic separation turntable 1230 can maintain a relatively constant reaction temperature. At least part of the circumferential direction of the inner and outer discs... Configuration slots are provided, with at least some of them located at corresponding intervals on the inner and outer disks. Magnetic components with the same magnetic properties are configured in the corresponding configuration slots. Preferably, the N poles of all magnetic components are arranged close to the reaction cup 100. Alternatively, the S poles of all magnetic components can be arranged close to the reaction cup 100 to apply a magnetic field to the reaction cup 100 within the magnetic separation turntable 1230 from both inside and outside directions. This allows the magnetic beads within the reaction cup 100 to drive the bridged target detection material to adhere to the wall of the reaction cup 100, facilitating subsequent aspiration of the cleaning liquid by the aspiration needle. The operating unit includes a suction needle connecting base plate 121, a push rod connecting base plate 123, and a dispensing connecting base plate 122 arranged sequentially along the height direction. An operating unit configuration base plate 124 is also arranged at the lower part of the magnetic separation disk. The suction needle connecting base plate 121 is located at the top layer and is used to install the suction needle and drive the suction needle to rise and fall independently. The push rod connecting base plate 123 is located between the suction needle connecting base plate 121 and the dispensing connecting base plate 122. A gripper push rod 1260 is installed on the push rod connecting base plate 123. The gripper push rod 1260 is arranged coaxially with the dispensing assembly and can be inserted into the dispensing assembly and act on the gripper of the cup rotating part 1200. The dispensing connecting base plate 122 is located below the push rod connecting base plate 123 and is used to install the dispensing assembly and drive the dispensing assembly to rise and fall as a whole. To ensure the stability of the top-layer suction needle connecting base plate 121 during movement and to prevent interference with other layer operation units, the suction needle connecting base plate 121 is configured to be driven to rise and fall independently relative to the device body. An upwardly extending support structure is provided on one side of the operation unit configuration base plate 124. The suction lifting drive motor 12111 is fixedly mounted on the support structure of the operation unit configuration base plate 124. One end of the suction lifting transmission screw 12112 is connected to the suction lifting drive motor 12111, and the other end is connected to a horizontal extension block at the top of the support structure. The suction needle connecting base plate 121 is threadedly connected to the suction lifting transmission screw 12112, so that the suction needle connecting base plate 121 can independently rise or fall through the forward or reverse rotation of the suction lifting drive motor 12111.A plurality of suction needles for aspirating cleaning liquid are fixedly connected to the suction needle connecting base plate 121. Here, there are four suction needles, namely the first-order suction needle 1201, the second-order suction needle 1202, the third-order suction needle 1203, and the fourth-order suction needle 1204 mentioned above. The spacing between each pair of suction needles is the same, which makes the operation and control of the device body simpler. In order to reduce reliability problems such as bending, blockage and breakage of suction needles due to obstruction, each suction needle and the suction needle connecting base plate 121 also includes an elastic element, which can compress the elastic element when the suction needle is obstructed, thereby protecting the suction needle and avoiding the aforementioned problems. The push rod connecting base plate 123 is movably connected to the dispensing connecting base plate 122, so that the push rod connecting base plate 123 can rise and fall synchronously with the dispensing connecting base plate 122 relative to the device body. The push rod connecting base plate 123 can also be driven to rise and fall independently relative to the dispensing connecting base plate 122, thereby driving the gripper push rod 1260 to rise and fall independently within the injection channel. The push rod connecting base plate 123 and the dispensing connecting base plate 122 are guided by several first guide rods; preferably, two first guide rods are arranged here. The push rod connecting base plate 123 can also be raised and lowered by push rods. The drive mechanism enables independent lifting and lowering relative to the filling connection base plate 122. The push rod lifting drive mechanism includes a push rod lifting drive motor 1262 connected to the push rod connection base plate 123, and a push rod lifting transmission screw 1261 that passes through the push rod connection base plate 123 and is rotatably limited to the filling connection base plate 122 via a rotating shaft at its bottom end. The push rod lifting drive motor 1262 generates relative rotational motion with the push rod lifting transmission screw 1261 by means of a drive current, thereby driving the push rod connection base plate 123 to lift and lower relative to the filling connection base plate 122 through threaded engagement. The push rod connection base plate 123 is connected to at least two gripper push rods 1260; here, there are four gripper push rods 1260, which, in conjunction with four cup-gripping rotating parts 1200, can perform clamping and gripping operations on the reaction cup 100.The filling connection base plate 122 is configured to be driven to independently raise and lower the filling assembly relative to the lower device body. The filling connection base plate 122 and the operation unit mounting base plate 124 are guided by several second guide rods. Preferably, three second guide rods are spaced apart between them. The filling lifting drive motor is mounted on the filling connection base plate 122. The filling lifting transmission screw passes through the filling connection base plate 122 and its lower end is connected to the magnetic separation disk. Preferably, the lower end of the filling lifting transmission screw is fixedly connected to a specific position on the inner disk and can be configured as a rotatable movable connection via a shaft or the like. The filling lifting drive motor generates relative rotational motion with the filling lifting transmission screw through a drive current, thereby driving the filling assembly through threaded engagement. The filling connection base plate 122 can be raised and lowered independently. When the filling connection base plate 122 is raised and lowered, the push rod connection base plate 123 is raised and lowered synchronously through the movable connection relationship. The filling connection base plate 122 is connected to no less than two filling components. The number of filling components can be set to four to cooperate with four gripping cup rotating parts 1200 to hold the reaction cup 100 and then add cleaning fluid. In order to add substrate more accurately and efficiently, a substrate filling component is also provided here in cooperation with the substrate needle 1215. In order to reduce the difficulty of system design and ensure better system maintainability, the structure and configuration of the substrate filling component and the filling component are basically the same. The reaction cup 100 in the receiving position can be driven to rotate to different positions so that it can cooperate with the filling component and the aspiration needle to perform cleaning fluid filling and aspiration operations respectively.

[0044] A lifting transmission block is fixedly connected to the suction needle connecting base plate 121. An internal thread is provided in the center hole of the lifting transmission block. The suction lifting transmission screw 12112 passes through the lifting transmission block, and the two are threadedly connected. The end of the suction lifting transmission screw 12112 is connected to the suction lifting drive motor 12111. Thus, the forward or reverse rotation of the suction lifting drive motor 12111 can drive the suction needle connecting base plate 121 to rise or fall. To precisely constrain the lifting movement, several third guide rods are connected between the push rod connecting base plate 123 and the filling connecting base plate 122; here, two third guide rods are used. The push rod lifting transmission screw 1261 passes through the push rod connecting base plate 123 and is constrained by the filling connecting base plate 122. Here, the bottom end of the push rod lifting transmission screw 1261 is rotatably connected to and constrained by the filling connecting base plate 122 via a bearing. The push rod lifting transmission screw 1261 passes through the push rod lifting drive motor 1262 fixedly connected to the push rod connecting base plate 123, thus enabling the push rod to rise and fall. The drive motor 1262 can generate relative rotational motion between the motor and the lead screw by relying on the drive current, and then drive the push rod connecting base plate 123 to move up and down through the threaded connection. Several first guide rods are also fixedly connected between the push rod connecting base plate 123 and the filling connecting base plate 122; here, two first guide rods are shown. Several second guide rods are arranged between the operating part mounting base plate 124 and the filling connecting base plate 122; here, three second guide rods are shown spaced apart between them. The filling lifting transmission lead screw passes through the filling connecting base plate 126. 22 is connected to the lower magnetic separation disk. The lower end of the filling lifting transmission screw is fixedly connected to a specific position of the inner disk, and can be configured as a rotatable movable connection through a rotating shaft, etc. The filling lifting transmission screw passes through the filling lifting drive motor fixedly connected to the filling connecting base plate 122. In this way, the filling lifting drive motor can generate relative rotational motion between the motor and the screw by relying on the drive current. Then, through the threaded connection, the filling connecting base plate 122 can be driven to move up and down. The filling lifting drive motor can generate relative rotational motion between the filling lifting drive motor and the filling lifting transmission screw by relying on the drive current. Then, through the threaded connection between the filling lifting transmission screw and the filling connecting base plate 122, the filling head connecting base plate is driven to move up and down. While the filling connecting base plate 122 is driven to move up and down, it can drive the push rod connecting base plate 123 to move up and down synchronously, making the motion drive of the entire operating part simpler. At the same time, the various functional units integrated on the upper part can be driven independently, making the operating part more flexible. The composite drive of multiple motors also gives the upper operating part a higher degree of freedom and functional integration.

[0045] After the mixture in reaction cup 100 has completed the incubation reaction, the reaction cups 100 can be transferred one by one to the magnetic separation and cleaning device 12 to complete cleaning and substrate addition operations, forming a mixed liquid system suitable for testing. The reaction cups 100 in the receiving position of the magnetic separation turntable 1230 in the magnetic separation and cleaning module are driven to rotate to the first-stage injection station 1231 to add a preset amount of cleaning liquid with the first-stage injection needle 1211. Here, the first-stage injection needle 1211 can be fixedly connected to the injection connection base plate 122 by a connecting block. In this way, the first-stage injection needle 1211 can move up and down with the injection connection base plate 122. In order to ensure the accuracy of the first-stage injection and reduce the possibility of splashing and other contamination during the injection process, the first-stage injection needle 1211... The portion of the liquid that passes through the filling connection substrate 122 and is exposed is longer than the length of the injection needle inside the filling assembly but shorter than the length of the suction needle. After filling, the magnetic separation turntable 1230 can be driven to rotate, causing the reaction cup 100 to rotate intermittently through several slots for configuring magnetic components. This magnetic attraction of magnetic beads and bridging target materials to the wall of the reaction cup 100 is repeated several times. As the rotation continues, the reaction cup 100 is driven to engage with the first-order suction needle 1201, i.e., the reaction cup 100 is driven to engage with the first-order pipetting station 1241. At this point, the first-order suction needle 1201 can be lowered by the suction lifting drive motor 12111 to insert into the reaction cup 100. It can be inserted from a position near the center line to a specific position below the liquid surface. Subsequently, as the liquid aspiration process proceeds, the height of the aspiration needle gradually decreases with the drop in liquid level until aspiration is complete. The first-stage aspiration needle 1201 is then driven upwards to detach from the reaction cup 100. The magnetic separation turntable 1230 is driven to continue rotating until it engages with the second-stage injection station 1232. At this point, the injection lifting drive motor drives the injection assembly to lower its height to at least a portion of the mouth of the reaction cup 100. The injection assembly then clamps and lifts the reaction cup 100, allowing the cleaning fluid to be injected into the reaction cup 100 while connected to the injection assembly. This lowers the outlet position of the cleaning fluid during injection, preventing splashing due to excessive injection height. To reduce the risk of splashing during cleaning fluid injection... Due to the magnetic influence during the injection process, the injection component can drive the reaction cup 100 to rise a preset distance before performing cleaning fluid injection and mixing. To achieve the combined functions of cleaning fluid injection and rotational mixing using the injection component, an injection needle fixing part is also connected to the injection connecting base plate 122. The injection needle connected to the injection needle fixing part can be at least partially inserted into the injection component, and the axis of the injection needle is aligned with the axis of the injection component. The injection component and the injection needle can move synchronously up and down driven by the injection connecting base plate 122. The injection connecting base plate 122 is then connected to the shampoo head rotation drive motor 12701. The output of the shampoo head rotation drive motor 12701 is connected to the transmission drive wheel 12702, and the transmission drive wheel 12702 is wound around the shampoo head rotation transmission belt 12703.The shampoo rotating drive belt 12703 is also wound around and connected to multiple shampoo rotating drive wheels 12704. This allows the same shampoo rotating drive motor 12701 to drive multiple shampoo rotating drive wheels 12704 to rotate. These shampoo rotating drive wheels 12704 can drive the cup-gripping rotating component 1200 within the dispensing assembly to rotate, causing the reaction cup 100 connected to the dispensing assembly to rotate. The multiple shampoo rotating drive wheels 12704 can drive multiple reaction cups 100 to rotate, efficiently mixing the magnetic beads and cleaning fluid within the reaction cup 100. To ensure that the shampoo rotating drive belt 12703 can be height-adjusted... To effectively tighten the belt, multiple tensioning pulleys 12705 are configured between the rotating drive wheels 12704. This configuration results in a lower cost and higher maintainability for the entire pulley drive mechanism. After the cleaning solution is added, the reaction cup 100 can be placed back into the magnetic separation turntable 1230. The adding component is driven back to the set position, and the magnetic separation turntable 1230 rotates so that the reaction cup 100 can cooperate with the second-stage aspiration needle 1202 to draw up the cleaning solution. This magnetic aspiration adding operation can be repeated. The cleaning operation can be repeated two, three, or more times to ensure that the target material after cleaning has higher purity and thus higher detection accuracy.

[0046] Depend on Figure 10 (a) Figure 10 (b) Figure 10 (c) As can be seen, this explanation uses the second-order injection needle 1212 in conjunction with the second-order injection position as an example. The second-order injection needle 1212 can be at least partially inserted into the filling assembly, and the tip of the second-order injection needle 1212 is at least partially exposed outside the outer shell of the filling assembly. The center of the gripper push rod 1260, which is coaxially arranged with the second-order injection needle 1212, allows the second-order injection needle 1212 to pass through. The bottom of the gripper push rod 1260 is equipped with a push head 12601. Preferably, the push head 12601 has a conical structure. The bottom of the shampoo head rotating drive wheel 12704 is connected to the connecting end of the cup gripping rotating component 1200 through an extension structure. The connecting end of 1200 is rotatably connected to the filling assembly via a rotating shaft. Thus, the rotational motion output by the shampoo rotating drive wheel 12704 can drive the connected cup-gripping rotating component 1200 to rotate. The other end of the cup-gripping rotating component 1200 opposite to the connecting end contains several separate grippers. The gripper push rod 1260 can be driven to move up and down, thereby enabling its push head 12601 to act on different positions inside the cup-gripping rotating component 1200. The push head 12601 can be driven to contact and act on the cup-gripping rotating component 1200, changing the opening of the grippers of the cup-gripping rotating component 1200 so that the grippers can hold the reaction cup 100 in the receiving position.

[0047] The specific injection process using the injection needle is as follows: Figure 11 (a) Figure 11As shown in (b), the dispensing assembly is driven to lower its height to pick up the reaction cup 100 inside the magnetic separation turntable 1230. When the dispensing assembly approaches the reaction cup 100, the gripper push rod 1260 can be driven to move and contact the cup gripping rotating member 1200. The opening of the gripper changes so that the gripper can hold the top opening of the reaction cup 100. After picking up the reaction cup 100, the gripper push rod 1260 can rise, and the gripper retracts inward under its own elasticity to clamp the connected reaction cup 100, thus completing its picking operation. The grippers here are made of metal or non-metallic materials with specific strength, and have the elastic characteristic of deforming under pressure. The top of the gripper is connected to the connecting end, and the inner walls of multiple grippers cooperate to form an action area. In the natural state, the area of ​​the action area gradually decreases from top to bottom. When the push head 12601 at the bottom of the gripper push rod 1260 extends into the action area, the inner wall of the gripper is pressed against the push head 12601. As the height of the push head 12601 gradually decreases, the gripper is pushed outward radially, generating elasticity. The deformation causes the gripper to open, allowing the top of the reaction cup 100 to extend into it. Then, as the pusher head 12601 rises, the gripper returns to its original position due to its elasticity. At this point, the bottom of the gripper abuts against the outer top wall of the reaction cup 100, using its own elastic force to hold the reaction cup 100. The connected reaction cup 100 can then be driven to rotate and / or filled with cleaning fluid by the second-stage injection needle 1212. After the cleaning fluid filling is complete, the gripper pusher 1260 can be driven again to contact the cup-gripping rotating component 120. The opening of the gripper is increased to release the reaction cup 100 into the magnetic separation turntable 1230. Preferably, in order to improve the maintainability of the module, the operating station adjacent to the last-stage aspiration needle is configured as the substrate filling station 1235. The substrate filling mechanism and filling components of the substrate filling station 1235 are assembled and matched with basically the same unit, except that the central injection needle is replaced with the substrate needle 1215. After the substrate filling is completed, the magnetic separation turntable 1230 drives the reaction cup 100 to rotate to the output port to complete the output.

[0048] To verify whether adding cleaning fluid to the reaction vessel affects the final test results, this invention designed two different experiments and conducted two tests to verify that the state of the reaction vessel when adding cleaning fluid has a specific impact on the test results. The results are shown in Table 1 below.

[0049] Table 1. Effects of different reaction vessel conditions on the test results when combined with the addition of cleaning solution.

[0050] Note: In condition 1, the magnetic bead concentration is 0.2 mg / ml and the glycerol concentration is 0%; in condition 2, the magnetic bead concentration is 0.2 mg / ml and the glycerol concentration is 20%; in condition 3, the magnetic bead concentration is 0.8 mg / ml and the glycerol concentration is 0%; in condition 4, the magnetic bead concentration is 0.8 mg / ml and the glycerol concentration is 20%. The one-step detection method of CZ1 refers to the reaction cup being transferred to the magnetic separation module for purification and cleaning after incubation in the reaction plate, and then detected. The two-step method of CZ2 refers to the reaction cup being transferred to the magnetic separation module for purification and cleaning after incubation in the reaction plate, then transferred back to the magnetic separation module for secondary magnetic separation and cleaning, and finally transferred to the detection module for detection.

[0051] Based on the photon counts detected by the optical detection system, adding cleaning fluid to the reaction cup while it is stationary and then rotating the cup, repeating the cleaning process several times before detecting the cleaned target material, results in a relatively low photon count and low cleaning and purification efficiency. However, rotating the reaction cup first and then adding cleaning fluid allows the cleaning fluid to rinse different parts of the cup, ensuring thorough cleaning of the target material connected to the magnetic beads. The rotating cup also helps create fluid vortices, reducing splashing during cleaning fluid addition. Although the subsequent process is essentially the same, adding cleaning fluid after rotation achieves higher cleaning and purification efficiency and a higher retention rate of effective components compared to adding cleaning fluid in a stationary reaction cup. Therefore, the overall number of detected photons is significantly improved, which is highly beneficial for improving the accuracy of photodetector-free detection, especially for high-speed diagnostic equipment. The magnetic separation turntable dwell time in different functional operating positions is also shorter, typically between 6 and 11 seconds.

[0052] like Figure 12As shown, the first transfer mechanism 211 can transfer the incubated reaction cup 100 to the receiving position of the reaction cup 100 within the magnetic separation turntable 1230. When the magnetic separation turntable 1230 rotates and moves the reaction cup 100 to the first-stage injection station 1231, the first-stage injection needle 1211 adds cleaning fluid into the reaction cup 100. The magnetic separation turntable 1230 rotates and moves the reaction cup 100 to at least one magnetic attraction position to perform magnetic adsorption of the magnetic beads within the reaction cup 100. Here, to ensure sufficient magnetic adsorption, the rotation and dwell time interval can be... The short interval of 6-11 seconds significantly shortens the detection time compared to the 15-second or even 20-second intervals in existing technologies. Simultaneously, the double-sided magnetic adsorption facilitates stronger adsorption and more accurate detection. After magnetic adsorption, the magnetic separation turntable 1230 moves the reaction cup 100 to the first-stage pipetting station 1241, where the first-stage aspiration needle 1201 aspirates the waste liquid from the reaction cup 100. The magnetic separation turntable 1230 then moves the reaction cup 100 to the second-stage injection station 1232, where the second-stage injection... The liquid injection needle 1212 adds cleaning fluid into the reaction cup 100. The cup-gripping rotating component 1200, coaxially arranged with the second-stage injection needle 1212, rotates the held reaction cup 100. Then, the second-stage injection needle 1212 adds cleaning fluid into the rotating reaction cup 100. This process is repeated several times with magnetic attraction and transfer until the magnetic separation turntable 1230 rotates, moving the reaction cup 100 to the nth-stage pipetting position. The nth-stage suction needle then removes the waste liquid from the reaction cup 100. Finally, the magnetic separation turntable 1230 moves the reaction cup 100 to the substrate. At the filling station 1235, the substrate needle 1215 adds substrate into the reaction cup 100. The cup-gripping rotating component 1200, which is coaxially arranged with the substrate needle 1215, drives the held reaction cup 100 to rotate. Then, the substrate needle 1215 adds substrate into the rotating reaction cup 100. After the substrate is added, the magnetic separation turntable 1230 rotates and drives the reaction cup 100 to the removal position. The first transfer mechanism 211 removes the reaction cup 100 with added substrate from the magnetic separation cleaning device 12. Preferably, n can be 3, 4, 5, etc. in this process.

[0053] like Figure 13 , Figure 14As shown, this embodiment is laid out within a rectangular three-dimensional space. A cleaning fluid storage unit is located at the bottom of this space, and it is fluidly connected to the upper operating platform via a flow pipeline. This allows the equipment to automatically and continuously perform different functional operations. The space below the operating platform can be isolated into multiple independent storage spaces to receive various cleaning fluids. A vacuum pump and a connected waste liquid recovery tank can also be installed, enabling larger-scale waste liquid recovery and ensuring more continuous and longer-term operation of the equipment. The operating platform includes a reagent carrying device 10, which contains... The rotating disk structure is driven to rotate, and multiple reagent rack receiving slots 1011 arranged circumferentially are arranged on the rotating disk. A magnetic bead mixing gear is fixedly arranged at the center of the reagent carrying device 10. Each reagent rack receiving slot 1011 has a hollow part arranged near the center position. The reagent tube rack can be detachably installed in the reagent tube rack receiving slot, and the position closest to the center is arranged as a magnetic bead reagent bottle receiving position, which can movably receive a magnetic bead reagent bottle equipped with magnetic bead liquid. Here, the bottom of the magnetic bead reagent bottle is formed with a bottle bottom gear, and the rotation axis of the bottle bottom gear is aligned with the rotation axis of the magnetic bead reagent bottle. In the overlapping configuration, after the reagent tube rack is placed into the reagent tube rack receiving slot, the bottom gear of the bottle can pass through the hollow part and mesh with the magnetic bead mixing gear. This allows the rotating disc to drive the reagent tube rack to rotate around its center, ensuring that the various reagents within the reagent tube rack are thoroughly mixed. Furthermore, the magnetic bead reagent bottle, relying on the meshing of the bottom gear, also rotates around its own axis, using a greater mixing driving force to thoroughly mix and suspend the magnetic bead reagent. To ensure long-term reagent preservation, the reagent carrying device 10 is also equipped with a cooling source, and the controller enables the reagent carrying device 10 to... To maintain a suitable refrigeration temperature and ensure a constant low-temperature environment within the reagent carrier 10, a reaction incubation device 11 is arranged adjacent to the reagent carrier 10. Multiple reaction cups 100 are arranged circumferentially within the reaction incubation device 11. A reagent dispensing probe 201 is positioned between the reagent carrier 10 and the reaction incubation device 11. A reagent needle cleaning mechanism is also provided between the reagent carrier 10 and the reaction incubation device 11. After multiple reagent dispensing operations, the reagent dispensing probe 201 can be driven to engage with the reagent probe cleaning mechanism to perform a cleaning operation.At the rear end of the operating table, a magnetic separation and cleaning device 12 and a signal detection device 13 are also configured. The rotating shaft 1605 of the first transfer mechanism 211 is configured within the quadrilateral area formed by the reagent carrier 10, the reaction incubation device 11, the magnetic separation and cleaning device 12, and the signal detection device 13. This allows the rotating shaft 1605 to drive the first transfer mechanism 211 to rotate within a travel range covering at least a portion of the reaction incubation device 11, the magnetic separation and cleaning device 12, and the signal detection device 13, thereby transferring the reaction cup 100 to perform different functional operations. A rotating mixing mechanism 16 is also arranged on the operating table, positioned between the reaction incubation device 11 and the signal detection device 13, and causing the rotating... The mixing mechanism 16 is positioned closer to the signal detection device 13, allowing the rotational stroke of the first transfer mechanism 211 to more precisely cover the mixing mechanism 16. A sample tube rack transfer section 152 is also located near the innermost part of the operating table, capable of transferring sample tube racks containing the samples to be analyzed via at least one transfer rail. The layout of the parallel transfer rails is illustrated here. This innermost placement also minimizes the risk of contamination, allowing for the shortest possible input and output of sample tube racks within the device. The sample dispensing probe 202 is positioned in the innermost region of the device, its rotational trajectory covering at least a portion of the transfer rails and the reaction incubation device 11. A sample dispensing probe 202 cleaning mechanism is also arranged at one of these locations. This allows for a cleaning process after each sample pipetting, minimizing the risk of contamination during sample transfer. During operation, the reagent dispensing probe 201 is driven to rotate and transfer reagents and / or magnetic beads from the reagent tray to the reaction cup 100 within the reaction incubation device 11. Similarly, the sample dispensing probe 202 is driven to rotate and transfer samples from the sample tube on the transfer rail to the reaction cup 100 within the reaction incubation device 11. The reaction incubation device 11 continues to rotate, allowing the reaction cup 100, after sample and reagent dispensing, to be rotated to engage with the first transfer mechanism 211 for clamping and transfer. The rotating mixing mechanism 16 receives samples from the reaction incubation device 11 collected by the first transfer mechanism 211. The reaction cup 100 is taken and rotated to mix the sample and reagent mixture dispensed inside. The first transfer mechanism 211 transfers the mixed reaction cup 100 back into the reaction incubation device 11. A heat source can be configured in the reaction incubation device 11 to maintain a suitable incubation temperature to promote the rapid occurrence of the incubation reaction. After the incubation reaction is completed, the reaction cup 100 can be transferred to the magnetic separation and cleaning device 12 for cleaning and separation and substrate addition. The first transfer mechanism 211 can also transfer the reaction cup 100 from the magnetic separation and cleaning device 12 to the signal detection device 13. The signal detection device 13 includes a light signal acquisition unit 130 to detect whether there is a target substance in the reaction cup 100 or to quantify the target substance content.The operating table also includes a reaction cup storage section 141, which can batch load reaction cups 100. The reaction cups 100 can be loaded by tilting, making the loading operation simple and quick. A reaction cup lifting mechanism 142 and a reaction cup transfer section 143 are arranged in conjunction with the reaction cup storage section 141. The reaction cup lifting mechanism 142 can pick up the reaction cups 100 in the reaction cup storage section 141 and lift them. One end of the reaction cup transfer section 143 is connected to the reaction cup lifting mechanism 142 to receive the lifted reaction cups 100, and the other end is connected to a cup sorting turntable 144. The cup sorting turntable 144 includes multiple receiving positions arranged in a circumferential direction, which can receive reaction cups 100 one by one. The sorting and transfer principle of the reaction cups 100 is not described in detail here. A second transfer mechanism 212 is also configured between the cup sorting turntable 144 and the reaction incubation device 11. The second transfer mechanism 212 can rotatably transfer the reaction cups 100. The empty reaction cup 100 is transferred to the reaction incubation device 11. The second transfer mechanism 212 is also specifically configured to transfer the empty reaction cup 100, reducing contamination while ensuring the automated loading efficiency of the reaction cup 100. The operating table also includes a sample tube rack buffer section 151, which contains multiple sample tube rack buffer slots arranged side by side along the depth of the equipment. This allows a specific number of samples to be tested to be buffered on the side of the equipment, enabling the equipment to be configured as a batch processing pipeline system, achieving the goal of high-speed continuous sample processing. In conjunction with the sample tube rack buffer section 151, a tube rack transfer mechanism 17 that can slide along the depth of the equipment is also arranged. The tube rack transfer mechanism 17 can pick up a sample tube rack in one of the sample tube rack buffer slots and transfer it to the transfer rail of the sample tube rack transfer section 152, thus realizing fully automated sample tube rack buffering and short-stroke flow operation within the equipment.

[0054] like Figure 15As shown, after the sample is added to the reaction cup 100 in the reaction incubation device 11, it can rotate to a position that can cooperate with the first transfer mechanism 211. At this time, the first transfer mechanism 211 can pick up the reaction cup 100 in the reaction incubation device 11 and rotate it around its axis to cooperate with the rotary mixing mechanism 16. The rotary mixing mechanism 16 includes a rotating cup position 160 that can receive the transferred reaction cup 100. The rotating cup position 160 is connected to a rotating shaft 1605, which is fixedly connected to the bottom of the rotating cup position 160, so that the rotating shaft is driven to rotate. The rotating cup 160 is able to rotate, thus thoroughly mixing the reagent and sample mixture within it. A driven wheel 1604 is sleeved on the rotating shaft 1605. The driven wheel 1604 is fixedly sleeved on the opposite end of the rotating shaft 1605 connected to the rotating cup 160. A driven wheel 1602 is positioned at a predetermined distance from the driven wheel 1604. A rotating transmission belt 1603 is sleeved between the two wheels. The driven wheel 1602 is also connected to the output shaft of a rotating motor 1601. Thus, the rotation of the motor output shaft can be achieved through the belt drive mechanism and the rotating shaft 1605. The transmission mechanism drives the rotating cup 160 to rotate. The diameter of the rotating drive wheel 1602 is no less than twice the diameter of the rotating driven wheel 1604. This larger transmission ratio allows the rotating cup 160 to rotate at a higher speed, enhancing the mixing effect of the mixture within the reaction cup 100 and reducing the impact of vibration during mixing. This design achieves higher mixing speeds using a simple transmission mechanism. Preferably, to ensure precise adjustment of important parameters such as the drive current of the output motor, a sensing block 1606 is also fixedly connected to the rotating shaft 1605. A rotation sensor 1607 is fixedly connected at a set position of the rotation mixing mechanism 16. When the rotating shaft 1605 drives the connected sensing block 1606 to rotate, it can be intermittently engaged between the transmitting end and the receiving end of the rotation sensor 1607. Therefore, the rotation speed of the rotating shaft can be accurately detected by the cooperation of the two, thereby realizing precise control of the rotation speed of the rotating cup position 160. The reaction cup 100 that has completed the rotation mixing can be picked up by the first transfer mechanism 211 and rotated back into the reaction plate to perform an incubation reaction at a specific temperature, such as constant temperature incubation at 37°C.

[0055] like Figure 16The signal detection device 13 shown cooperates with the optical signal acquisition unit 130 to perform the detection state diagram. The reaction cup 100 taken from the magnetic separation cleaning device 12 by the first transfer mechanism 211 can be rotated and transferred to the signal detection device 13. Here, the signal detection device 13 has a plurality of reading positions 1301 arranged in the circumferential direction to receive the transferred reaction cup 100. The bottom of the signal detection device 13 includes a reading rotation drive motor 1321. The output shaft of the motor is connected to the reading rotation drive wheel 1322. The drive wheel is wound with a reading rotation transmission belt 1323. The transmission belt is also connected to a reading rotation driven wheel 1604 arranged coaxially with the signal detection device 13. In this way, the reading rotation drive motor 1321 can drive the reaction cup 100 to rotate. The signal detection device 13 rotates around an axis. A light signal acquisition unit 130 is fixedly connected to one side of the signal detection device 13. The side of the signal detection device 13 has multiple reading holes 131 corresponding to reading positions 1301, allowing the light signal acquisition unit 130 to acquire signals from the reaction vessel 100 through the reading holes 131. Preferably, the side of the signal detection device 13 may be equipped with a heat-insulating and light-blocking layer. As the inner layer rotates, the multiple reading positions 1301 pass through the light signal acquisition unit 130 one by one to acquire their internal optical signals. A signal detection device cover is also fitted to the top of the signal detection device 13. The signal detection device cover has a loading / unloading hole located away from the light signal acquisition unit 130. The distance between the pipette orifice, the loading / unloading orifice, and the light signal acquisition unit 130 is smaller than the distance between the loading / unloading orifice and the light signal acquisition unit 130. This ensures that the signal detection device 13 maintains a darkroom environment. Loading the reaction cup 100 and aspirating waste liquid will not interfere with the detection. There is no risk of inaccurate detection results due to light leakage caused by the small distance between the functional orifices and the detection unit. Furthermore, there are no influencing factors such as vibration caused by transfer during the detection process. The pipette orifice is equipped with a waste liquid aspiration mechanism 203 that can be driven to move up and down. This mechanism includes a waste aspiration needle driven by a pipette arm. The pipette arm can be driven to move up and down by the waste aspiration lifting and lowering mechanism, allowing the waste aspiration needle to insert into or move away from the reaction cup 100 at the reading position 1301. The drive motor 2031 output is connected to the waste suction drive wheel 2032, and a waste suction transmission wheel 2034 is fixedly set at a preset distance between them. The two are wound together with a waste suction transmission belt 2033. The waste suction transmission belt 2033 is connected to the lifting rod of the pipette arm through a fixing member and moves up and down. The waste suction lifting drive mechanism lowers the height of the waste suction needle so that the waste liquid in the reaction cup 100 can be more reliably concentrated and collected. The reaction cup 100 that has completed waste suction can be driven by the signal detection device 13 to rotate to the position of the first transfer mechanism 211. The first transfer mechanism 211 takes in the reaction cup 100 that has completed waste suction from the signal detection device 13 through the loading and unloading hole, and finally collects the used reaction cup 100 into the waste recycling drawer through the waste recycling port.

[0056] This document uses specific embodiments to illustrate the principles and implementation methods of the present invention. The descriptions of these embodiments are only intended to aid in understanding the method and core ideas of the present invention. It should be noted that those skilled in the art can make various improvements and modifications to the present invention without departing from its principles, and these improvements and modifications also fall within the scope of protection of the claims of the present invention.

[0057] In the description of this invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship in which the product of this invention is usually placed during use. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0058] In the description of this invention, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

Claims

1. A dispensing component, characterized in that, include: A cup-gripping rotator is used to grip the reaction cup and drive the reaction cup to rotate around its own axis. A liquid injection channel is provided at the central axis of the cup-gripping rotator. The injection needle is coaxially arranged with the rotating gripper cup. The injection needle can extend into the injection channel and act on the reaction cup. The injection needle is used to inject cleaning fluid into the reaction cup.

2. The filling component according to claim 1, characterized in that, The cup-gripping rotating component includes a connecting end and multiple grippers. Multiple grippers are spaced apart at the connecting end and extend downward. The inner walls of the multiple grippers together form a working area for holding the reaction cup. The liquid injection channel is formed at the central axis of the connecting end and the multiple grippers. The axis of the injection needle coincides with the axis of the cup-gripping rotating component. When the cup-gripping rotating component clamps and drives the reaction cup to rotate, the injection needle injects cleaning fluid into the rotating reaction cup through the injection channel.

3. The filling component according to claim 2, characterized in that, Also includes: The gripper push rod is coaxially arranged with the cup-gripping rotating component and is movably inserted into the liquid injection channel; The bottom of the gripper push rod is provided with a push head; In its natural state, the radial dimension of the working area formed by the multiple grippers gradually decreases from top to bottom; When the gripper push rod is driven to move downward, the push head extends into the working area, and the outer peripheral surface of the push head pushes the inner wall of the multiple grippers radially outward. As the insertion depth of the push head increases, the grippers continuously undergo elastic deformation and open radially outward, thereby expanding the bottom opening of the working area to allow the top of the reaction cup to enter the working area. When the gripper push rod is driven to move upward, the push head retracts from the action area, and the multiple grippers retract radially inward under their own elastic restoring force, and clamp the reaction cup by abutting the top outer wall of the reaction cup through the bottom end of the gripper; when the gripper clamps the reaction cup, the bottom end of the gripper is in an elastic deformation state, and at this time the opening of the bottom end of the gripper is greater than its opening in the natural state.

4. A filling assembly according to claim 3, characterized in that, The gripper push rod moves independently relative to the cup-gripping rotating component to drive the multiple grippers to open or close. The injection needle moves up and down independently relative to the gripper push rod and the cup rotating component. The injection needle descends and extends into the reaction cup to inject cleaning fluid.

5. A filling assembly according to claim 3, characterized in that, It also includes a refilling rotary drive mechanism, which includes a refilling rotary drive motor, multiple refilling rotary drive wheels, and a refilling rotary transmission belt; Multiple filling rotary drive wheels are connected to the connecting end of the cup gripping rotating component and can drive the cup gripping rotating component to rotate synchronously; the axial center of the multiple filling rotary drive wheels is provided with a liquid injection channel, and the gripper push rod passes through the liquid injection channel and acts on the gripper; The filling rotary transmission belt is wound around and connected to multiple filling rotary drive wheels in a roundabout and zigzag manner, so as to drive the multiple filling rotary drive wheels to rotate synchronously; The filling rotation drive motor drives multiple filling rotation drive wheels to rotate via a filling rotation transmission belt, thereby causing the cup gripping rotating component to rotate synchronously with the clamped reaction cup.

6. A magnetic separation cleaning device, characterized in that, The device includes a main body and an operating unit. The operating unit is located above the main body and is used to perform cleaning liquid filling, waste liquid suction and displacement, and reaction cup clamping and rotation operations on the reaction cup inside the main body. The operating unit includes the filling component as described in any one of claims 1-5, and further includes a liquid suction needle connecting base plate, a push rod connecting base plate, and a filling connecting base plate arranged sequentially along the height direction. The suction needle connecting base plate is used to mount the suction needle and drive the suction needle to move up and down independently; A gripper push rod is mounted on the push rod connecting base plate. The gripper push rod is coaxially arranged with the dispensing assembly and can be inserted into the dispensing assembly and act on the gripper of the cup-gripping rotating part. The refilling connection base plate is used to install the refilling assembly and drive the refilling assembly to rise and fall as a whole.

7. The magnetic separation cleaning device according to claim 6, characterized in that, The filling connection base plate, push rod connection base plate, and suction needle connection base plate are all located above the device body and can be raised and lowered independently relative to the device body. The filling connection base plate is configured to be driven to cause the entire filling assembly to move independently up and down relative to the lower device body; The push rod connecting base plate is movably connected to the filling connecting base plate so that the push rod connecting base plate can move up and down synchronously with the filling connecting base plate relative to the device body, and the push rod connecting base plate can also be driven to move up and down independently relative to the filling connecting base plate, thereby driving the gripper push rod to move up and down independently in the liquid injection channel. The suction needle connecting base plate is configured to be driven to move independently relative to the device body.

8. The magnetic separation cleaning device according to claim 6, characterized in that, The device body includes: A magnetic separation disk includes a magnetic separation turntable that can rotate around an axis, and an inner disk and an outer disk that are fixedly disposed on the inner and outer sides of the magnetic separation turntable and are coaxial with the magnetic separation turntable; the magnetic separation turntable is provided with a plurality of receiving positions in the circumferential direction for receiving and driving the reaction cup to rotate. A plurality of configuration slots are spaced apart along the circumferential direction on the inner disk and the outer disk; A magnetic component is disposed within the configuration slot; The magnetic separation disk has several stations along its circumference. Each station is one of a dual-pole station, a single-pole station, or a non-magnetic-pole station. At the dual-pole station, both the inner and outer disks are provided with configuration slots, and the magnetic components in the configuration slots have the same magnetic polarity. At the single-pole station, either the inner or outer disk is provided with a configuration slot. At the non-magnetic-pole station, neither the inner nor outer disks are provided with configuration slots.

9. A control method for a dispensing component, applicable to the dispensing component according to any one of claims 1-5, characterized in that, The method includes the following steps: Grabbing steps: Control the entire filling component to descend and approach the reaction cup, and use the cup-grabbing rotating part to grab the reaction cup; Rotation step: Drive the gripping cup rotating component to rotate around its own axis, thereby causing the clamped reaction cup to rotate synchronously, so that the liquid contained in the reaction cup is driven into the rotation dynamic. Addition steps: While the reaction cup is in a rotating state, control the injection needle to extend downward into the reaction cup along the injection channel, and add cleaning fluid into the rotating reaction cup; Reset Procedure: After filling is complete, stop the injection and control the injection needle to reset. Control the cup gripper to stop rotating and use the cup gripper to return the reaction cup to the target position.

10. The control method for a filling component according to claim 9, characterized in that, The cup-gripping rotating component includes a connecting end and multiple grippers. The multiple grippers are spaced apart at the connecting end and extend downward. The inner walls of the multiple grippers together form an area for gripping the reaction cup. The injection channel is formed at the central axis of the connecting end and the multiple grippers. The filling assembly also includes a gripper push rod. The gripper push rod is coaxially arranged with the cup-gripping rotating component and is movably inserted into the injection channel. The bottom of the gripper push rod is provided with a push head. In its natural state, the radial dimension of the area formed by the multiple grippers gradually decreases from top to bottom. During the gripping step, the gripper push rod is driven to move downward, causing the pusher head to extend into the working area and push the inner walls of the multiple grippers radially outward, causing the grippers to elastically deform and open radially outward, allowing the top of the reaction cup to enter the working area; the gripper push rod is then driven to move upward, causing the pusher head to withdraw from the working area, and the multiple grippers retract radially inward under their own elastic restoring force, clamping the reaction cup by abutting the top outer wall of the reaction cup with the bottom of the grippers; In the reset step, the gripper rotating component is controlled to move the reaction cup above the target position, and the gripper push rod is driven to move downward relative to the gripper rotating component, so that the push head extends into the working area again and pushes the inner wall of the multiple grippers radially outward, causing the grippers to elastically deform and open radially outward, thus expanding the opening at the bottom of the working area. The bottom end of the gripper disengages from the top outer wall of the reaction cup, thereby releasing the reaction cup and placing it back to the target position. Then, the gripper push rod is driven to move upward, so that the push head withdraws from the working area, and the multiple grippers retract radially inward under their own elastic restoring force to return to their natural state. The entire dispensing assembly is then controlled to rise and reset.

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