A composition for combating gingival aging and its use

Abstract

The present application relates to a kind of anti-gum aging compositions and its application, the composition includes the following components: farnesol, ergothioneine, gynostemma pentaphyllum leaf extract, alder fruit extract and mussel extract, the five ingredients described above can realize the effect of antioxidation, anti-inflammatory, create favorable microenvironment for gum tissue repair, also can effectively synergistic inhibition collagen degradation, promote matrix regeneration, help to improve gum atrophy and loss of flexibility, to realize efficient, mild, long-acting anti-gum aging, and have long-acting oral mucosa retention capacity, can be widely adapted to toothpaste, mouthwash, tooth paste etc. Various oral care products.

Description

Technical Field

[0001] This invention relates to the technical field of oral products, and more specifically to a composition for combating gingival aging and its application. Background Technology

[0002] With age and deterioration of oral hygiene, gingival tissue gradually exhibits signs of aging such as atrophy, redness, bleeding, and decreased immunity. Current oral care products mostly focus on single anti-inflammatory or hemostatic functions, and their technological shortcomings are mainly reflected in the following aspects: First, anti-aging products often target only a single factor and lack effective regulation of extracellular matrix (ECM) homeostasis. One of the core pathological features of gingival aging is disordered collagen metabolism, manifested as overexpression of matrix metalloproteinases (MMPs, especially MMP-1) leading to collagen degradation, and a decreased ability of gingival fibroblasts to synthesize type I collagen (Col-I). Existing products often only focus on transient anti-inflammatory effects, rarely simultaneously inhibiting MMP-1 activity and promoting Col-I regeneration, thus failing to structurally improve gingival recession and loss of resilience.

[0003] Secondly, the synergistic effect of antioxidants and anti-inflammators is poor, making it difficult to break the vicious cycle of "inflammation-oxidation." Gingival tissue is constantly exposed to an oxidative stress microenvironment stimulated by bacterial plaque, and the accumulation of reactive oxygen species (ROS) further exacerbates the inflammatory response. Existing formulations mostly use single antioxidants or anti-inflammatory agents, which are insufficient to simultaneously and effectively eliminate free radicals inside and outside the mitochondria and inhibit the release of inflammatory factors (such as IL-1β and TNF-α), thus failing to slow down the tissue aging process at its source.

[0004] Secondly, the active ingredients have a short residence time in the moist oral environment and a low absorption rate through the mucosa. Single-function ingredients have poor stability and are easily diluted, washed away, or oxidized and inactivated by saliva, resulting in low bioavailability and making it difficult to effectively reverse collagen metabolism imbalance within the short brushing time.

[0005] Therefore, there is an urgent need for an anti-gingival aging composition that can simultaneously regulate the expression ratio of MMP-1 / Type I collagen, has both antioxidant and anti-inflammatory effects, and has a long-lasting oral mucosal retention capacity. Summary of the Invention

[0006] The purpose of this invention is to overcome the shortcomings of the prior art and provide a composition for anti-gingival aging and its application.

[0007] To achieve the above objectives, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides a composition for anti-gingival aging, comprising the following components: bisabolol, ergothioneine, Gynostemma pentaphyllum leaf extract, alder fruit extract and mussel extract; wherein the mass ratio of the bisabolol, ergothioneine, Gynostemma pentaphyllum leaf extract, alder fruit extract and mussel extract is (1-5):(0.1-2):(2-10):(1-6):(0.1-2).

[0008] Ergothionein is specifically enriched in the mitochondria of gingival cells via the OCTN1 transporter, effectively scavenging reactive oxygen species (ROS) and blocking cascade damage caused by oxidative stress; bisabolol significantly reduces the release of inflammatory factors such as IL-1β and TNF-α by inhibiting the COX-2 and NF-κB signaling pathways.

[0009] Gynostemma pentaphyllum leaf extract is rich in saponins, which can significantly promote the proliferation and migration of fibroblasts and upregulate the synthesis of type I collagen (Col-I). Alder fruit extract, with its excellent antibacterial and anti-inflammatory properties, reduces the irritation of gingival plaque toxins and helps inhibit the overexpression of matrix metalloproteinase-1 (MMP-1). Mussel extract is rich in mussel foot protein, which can effectively promote gingival collagen regeneration. Moreover, its unique DOPA group can undergo physicochemical adsorption in the moist oral environment, adhering tightly to the gingival mucosa surface and significantly prolonging the local retention time of the above-mentioned active ingredients. At the same time, the biocompatible film formed has a certain barrier function, which can reduce external irritation and act as an adhesion matrix to fix active ingredients, reducing the loss caused by saliva rinsing, thus solving the industry problem of low bioavailability of oral care products due to saliva rinsing.

[0010] In summary, the components of the composition of the present invention can synergistically achieve antioxidant and anti-inflammatory effects, create a favorable microenvironment for gingival tissue repair, and effectively inhibit collagen degradation and promote matrix regeneration, thus helping to improve gingival recession and loss of resilience.

[0011] Preferably, the mass ratio of bisabolol, ergothioneine, gynostemma pentaphyllum leaf extract, alder fruit extract and mussel extract is (1-3):(0.2-1):(3-8):(2-5):(0.5-2).

[0012] Most preferably, the mass ratio of bisabolol, ergothioneine, gynostemma pentaphyllum leaf extract, alder fruit extract and mussel extract is 2:0.5:5:3:1.

[0013] In a second aspect, the present invention provides the use of the anti-gingival aging composition of the first aspect in the preparation of oral products.

[0014] Preferably, the oral products include toothpaste, tooth powder, mouthwash, oral spray, and whitening strips; the amount of the anti-gingival aging composition added is 0.01-5%.

[0015] Thirdly, the present invention provides a toothpaste for combating gingival aging, comprising the following ingredients by weight percentage: 0.01-1% of the anti-gingival aging composition of the first aspect, 25-35% of an abrasive, 20-25% of a humectant, 12-20% of a surfactant, 1-2.5% of a flavoring agent, 0.5-1% of a thickener, 0.005-0.01% of a sweetener, and the balance being water.

[0016] Preferably, the friction agent is silicon dioxide.

[0017] Preferably, the moisturizer is sorbitol and / or propylene glycol.

[0018] Preferably, the surfactant is at least one of sodium lauroyl sarcosinate, cocamidopropyl betaine, and sodium dodecyl sulfate.

[0019] Preferably, the flavoring agent is a fragrance.

[0020] Preferably, the thickener is at least one of sodium carboxymethyl cellulose, xanthan gum, and guar gum.

[0021] Preferably, the sweetener is at least one of sucralose, xylitol, and erythritol.

[0022] Compared with the prior art, the beneficial effects of the present invention are as follows: The composition of this invention comprises five components: bisabolol, ergothioneine, gynostemma pentaphyllum leaf extract, alder fruit extract, and mussel extract. These components work synergistically to achieve antioxidant and anti-inflammatory effects, creating a favorable microenvironment for gingival tissue repair. They also effectively inhibit collagen degradation and promote matrix regeneration, helping to improve gingival recession and loss of resilience. Thus, it achieves highly efficient, gentle, and long-lasting anti-gingival aging, and has long-lasting oral mucosal retention capacity, making it widely compatible with various oral care products such as toothpaste, mouthwash, and whitening strips. Detailed Implementation

[0023] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0024] The sources of the raw materials used in the following examples and comparative examples are as follows: Bisabolol: from Yili Jiangning Biotechnology Co., Ltd. (a subsidiary of the applicant, Yili Chuanning Biotechnology Co., Ltd.); Ergothioneine: from Yili Jiangning Biotechnology Co., Ltd. (a subsidiary of the applicant, Yili Chuanning Biotechnology Co., Ltd.); Gynostemma pentaphyllum leaf extract: purchased from Guangzhou Jiufeng Trading Co., Ltd.; Alder fruit extract: purchased from Guangzhou Jiufeng Trading Co., Ltd.; Mussel extract: purchased from Shenzhen Baiyin Biotechnology Co., Ltd.; Unless otherwise specified, all other materials and reagents used in the examples are commercially available.

[0025] Example 1 An anti-gingival aging composition comprising bisabolol, ergothioneine, gynostemma pentaphyllum leaf extract, alder fruit extract and mussel extract in a mass ratio of 2:0.5:5:3:1, wherein the total mass parts of said bisabolol, ergothioneine, gynostemma pentaphyllum leaf extract, alder fruit extract and mussel extract are 100 parts.

[0026] Example 2 An anti-gingival aging composition comprising bisabolol, ergothioneine, gynostemma pentaphyllum leaf extract, alder fruit extract and mussel extract in a mass ratio of 1:0.2:3:2:0.5, wherein the total mass parts of said bisabolol, ergothioneine, gynostemma pentaphyllum leaf extract, alder fruit extract and mussel extract are 100 parts.

[0027] Example 3 An anti-gingival aging composition comprising bisabolol, ergothioneine, gynostemma pentaphyllum leaf extract, alder fruit extract and mussel extract in a mass ratio of 3:1:8:5:2, wherein the total mass parts of said bisabolol, ergothioneine, gynostemma pentaphyllum leaf extract, alder fruit extract and mussel extract are 100 parts.

[0028] Example 4 An anti-gingival aging composition comprising bisabolol, ergothioneine, gynostemma pentaphyllum leaf extract, alder fruit extract, and mussel extract in a mass ratio of 1:0.1:2:1:0.1, wherein the total mass fraction of said bisabolol, ergothioneine, gynostemma pentaphyllum leaf extract, alder fruit extract, and mussel extract is 100 parts.

[0029] Example 5 An anti-gingival aging composition comprising bisabolol, ergothioneine, gynostemma pentaphyllum leaf extract, alder fruit extract, and mussel extract in a mass ratio of 5:3:10:6:0.3, wherein the total mass fraction of said bisabolol, ergothioneine, gynostemma pentaphyllum leaf extract, alder fruit extract, and mussel extract is 100 parts.

[0030] Comparative Example 1 The only difference between Comparative Example 1 and Example 1 is that: Comparative Example 1 does not add bisabolol, but uses ergothioneine, Gynostemma pentaphyllum leaf extract, alder fruit extract and mussel extract in a mass ratio of 0.5:5:3:1 to make up for the missing amount.

[0031] Comparative Example 2 The only difference between Comparative Example 2 and Example 1 is that Comparative Example 2 does not add ergothionein, but uses bisabolol, Gynostemma pentaphyllum leaf extract, alder fruit extract and mussel extract in a mass ratio of 2:5:3:1 to make up for the missing amount.

[0032] Comparative Example 3 The only difference between Comparative Example 3 and Example 1 is that Comparative Example 3 does not add Gynostemma pentaphyllum leaf extract, but uses bisabolol, ergothioneine, alder fruit extract and mussel extract in a mass ratio of 2:0.5:3:1 to make up for the missing amount.

[0033] Comparative Example 4 The only difference between Comparative Example 4 and Example 1 is that Comparative Example 4 does not add alder fruit extract, but uses bisabolol, ergothioneine, gynostemma pentaphyllum leaf extract and mussel extract in a mass ratio of 2:0.5:5:1 to make up for the missing amount.

[0034] Comparative Example 5 The only difference between Comparative Example 5 and Example 1 is that Comparative Example 5 does not add mussel extract, but uses bisabolol, ergothioneine, gynostemma pentaphyllum leaf extract and alder fruit extract in a mass ratio of 2:0.5:5:3 to make up for the missing amount.

[0035] Comparative Example 6 The only difference between Comparative Example 6 and Example 1 is that the total mass fraction of the composition remains unchanged, and the mass ratio of the bisabolol, ergothioneine, gynostemma pentaphyllum leaf extract, alder fruit extract and mussel extract is 2:0.5:1:3:5.

[0036] Comparative Example 7 The only difference between Comparative Example 7 and Example 1 is that the total mass fraction of the composition remains unchanged, and the mass ratio of the bisabolol, ergothioneine, gynostemma pentaphyllum leaf extract, alder fruit extract and mussel extract is 2:3:5:0.5:1.

[0037] Test Example 1: Test of the upregulation rate of COL1 gene, the upregulation rate of SIRT1 gene, and the inhibition rate of MMP-1 gene expression in the composition. MTT is a method based on observing cellular metabolic activity. Live cell metabolism reduces the yellow, water-soluble MTT to insoluble blue-purple formazan. After dissolving formazan in DMSO, the optical density is measured using a microplate reader; the number of live cells is directly proportional to the optical density. Matrix metalloproteinases (MMPs) are a highly conserved class of enzymes throughout evolution, widely found in vertebrates and invertebrates. They are crucial enzymes essential for degrading the extracellular matrix, capable of degrading almost all extracellular matrix components, such as collagen and elastin. For example, MMP-1 specifically hydrolyzes type I, II, and III collagen, while MMP-3 degrades type IV collagen and activates other MMPs (such as MMP-1 / 7 / 9).

[0038] Sirtuin 1 (SIRT1), a type III protein deacetylase, is a core regulatory protein that controls oxidative homeostasis, inflammatory response, and cellular senescence in gingival cells. It directly participates in the entire process of gingival anti-inflammation, tissue repair, and anti-gingival aging. Normal, healthy gingival fibroblasts maintain a certain expression level of SIRT1, which can inhibit oxidative stress signaling pathways, reduce the secretion of pro-inflammatory factors, alleviate premature cell aging, and repair DNA damage caused by oxidation. When the gums are subjected to oxidative stress and inflammation, the expression level of SIRT1 decreases significantly, and the antioxidant, anti-inflammatory, and self-repair capabilities of gingival cells are greatly weakened, accelerating gingival aging and damage.

[0039] In healthy gingival tissue, the expression level of MMPs is extremely low. However, in the pathological process of oral problems such as periodontitis and gingival aging, oxidative stress and inflammatory factors can simultaneously trigger a triple damage effect: First, it significantly upregulates MMP expression and accelerates collagen degradation; second, it inhibits the synthesis of type I collagen (COL1), which serves as the scaffold of gingival connective tissue, thus destroying the gingival support structure; and third, it downregulates SIRT1 expression, resulting in the loss of endogenous anti-inflammatory, antioxidant, and anti-aging protective mechanisms. These three factors work together to accelerate the degradation of gingival connective tissue, gingival recession, and gingival fragility and aging.

[0040] tert-butyl hydroperoxide (TBHP) is a classic oxidative stress inducer that can stimulate human gingival fibroblasts to construct an in vitro cell model that simultaneously simulates gingival oxidative damage, inflammatory stress, and cellular senescence. After the test raw material or oral care product intervenes in the cells, changes in three key indicators can be simultaneously detected by real-time quantitative PCR (qRT-PCR): the level of SIRT1 gene expression recovery, the degree of MMP gene expression downregulation, and the level of type I collagen (COL1) gene expression recovery.

[0041] Among them, upregulating SIRT1 expression in the sample can achieve anti-inflammatory, antioxidant, and anti-aging effects on gingiva from the upstream; downregulating MMP-1 can reduce gingival collagen degradation; and upregulating COL1 can promote the regeneration of gingival connective tissue. The combination of these three effects can comprehensively evaluate the sample's overall efficacy in repairing gingiva, improving gingival health, and resisting gingival aging.

[0042] Gene expression levels in cells were determined by quantitative real-time PCR (qRT-PCR), a method that uses fluorescent chemicals to monitor the total amount of product after each PCR cycle during DNA amplification and uses internal or external reference methods to quantify specific DNA sequences in the test sample.

[0043] I. Cells and Reagents cell lines Human gingival fibroblasts (HGF) were purchased from Beijing Boruikangmu Biotechnology Co., Ltd.

[0044] Culture media and reagents 1) Complete culture medium: DMEM high glucose medium supplemented with 15% fetal bovine serum (FBS); 2) MTT working solution: Dilute thiazolyl blue (MTT) with complete culture medium and prepare fresh before use; 3) Oxidative stress modeling inducer: tert-butyl hydroperoxide (TBHP); 4) Positive control reagent: TGF-β1; 5) Samples to be tested: The compositions of Examples 1-5 and Comparative Examples 1-7 were diluted with complete culture medium to a sample solution with a mass concentration of 0.5%; 6) Washing solution: DPBS buffer; RNA extraction and cDNA synthesis kit.

[0045] II. Experimental Group Setup Four parallel experimental groups were set up, with biological replicates in each group: Blank control group (BC): Only DMEM high glucose complete medium was added, without TBHP induction, and without sample / positive control; Oxidative damage model group (M): culture medium + TBHP inducer, without adding test samples and positive controls; Positive control group (PC): culture medium + TBHP inducer + TGF-β1; Test sample group (S): culture medium + TBHP inducer + test samples at different concentration gradients.

[0046] III. Complete Test Operation Steps Step 1: Cell resuscitation and passage culture Following laboratory procedure WI-GZB-703, human gingival fibroblasts were resuscitated, passaged, and routinely cultured, maintaining a constant temperature environment of 37°C and 5% CO2. Cells in the logarithmic growth phase were selected for subsequent experiments.

[0047] Step 2: MTT cytotoxicity preliminary assay (screening for safe effective concentrations) Follow the WI-GZB-717 cytotoxicity testing procedure; The test samples were diluted to five concentrations: 0.01%, 0.05%, 0.2%, 0.5%, and 2%, and co-cultured with gingival fibroblasts at each concentration. After the culture was completed, MTT working solution was added for incubation, DMSO was used to dissolve the formazan crystals, and the OD absorbance of each well was measured using a microplate reader. Formula for calculating cell viability: Cell viability (%) = (OD of sample wells - OD of zeroing wells) ÷ (OD of blank control wells - OD of zeroing wells) × 100% Validity determination: The coefficient of variation (CV) of the OD values ​​of each of the three biological replicates was ≤20%, and the experiment was valid. The concentration of the sample with cell viability ≥85% was selected as the concentration for efficacy testing. In this embodiment, the maximum concentration of 0.5% that meets the cell viability range was selected for qRT-PCR efficacy testing.

[0048] Step 3: Cell seeding Human gingival fibroblasts in the logarithmic growth phase were digested and centrifuged. A homogeneous cell suspension was prepared using complete culture medium and seeded into 12-well cell culture plates. The plates were then incubated at 37°C in a 5% CO2 incubator for 24 h ± 2 h.

[0049] Step 4: Oxidative stress modeling + sample drug administration Discard the original culture medium on the culture plate and add the corresponding culture medium according to the experimental groups: the blank control group only has basal culture medium; the model group, positive control group and sample group all have culture medium containing TBHP inducer added, the positive control group is supplemented with TGF-β1, and each sample group has 0.5% concentration of the test sample solution added; after the sample is added, continue to culture at 37℃ and 5% CO2 for 24h±2h.

[0050] Step 5: Collection of cellular RNA samples After the culture was completed, the culture medium was discarded, the cells were washed once with DPBS, all cells were collected, and the cells were transferred to an enzyme-free operating table to extract total RNA.

[0051] Step 6: Reverse transcription to prepare cDNA Total RNA was extracted from cells and cDNA was synthesized through reverse transcription. Following the MMP-1 detection procedure, the cDNA product was diluted 1:1 by volume with DEPC water and set aside.

[0052] Step 7: Simultaneous detection of the relative expression levels of COL1 and MMP-1 gene mRNA by qRT-PCR Following the WI-GZB-721 quantitative PCR protocol, the type I collagen COL1 gene, matrix metalloproteinase MMP-1 gene, and quiescent information regulator SIRT1 gene were simultaneously amplified and detected. Amplification was performed using a real-time quantitative PCR instrument. Data uniformly adopts 2 -△△CT The relative expression levels of COL1, SIRT1, and MMP-1 genes were calculated using the method. The results showed that there was a statistically significant difference between the positive control group and the model group (P < 0.05), indicating that the model was successfully established.

[0053] Based on the relative expression levels of COL1, SIRT1, and MMP-1 genes, the following three indicators were used to calculate quantitative data: the upregulation rate of COL1 gene, the upregulation rate of SIRT1 gene, and the inhibition rate of MMP-1 gene expression.

[0054] Among them, C M The relative expression level of the COL1 gene in the model group; C S The relative expression level of the COL1 gene in the sample group to be tested; Among them, S M The relative expression level of the SIRT1 gene in the model group; S S The relative expression level of the SIRT1 gene in the sample group to be tested; Among them, M M The relative expression level of the MMP-1 gene in the model group; M S The values ​​represent the relative expression levels of the MMP-1 gene in the sample groups to be tested; specific data are shown in Table 1.

[0055] Table 1. Data on the upregulation rate of COL1 gene, the upregulation rate of SIRT1 gene, and the inhibition rate of MMP-1 gene expression for each composition. Positive control group 70.32 43.80 61.85 Example 1 64.29 38.65 57.52 Example 2 63.78 38.20 56.17 Example 3 63.67 38.34 56.35 Example 4 60.49 35.66 53.57 Example 5 61.12 36.80 54.88 Comparative Example 1 42.64 25.36 37.36 Comparative Example 2 40.22 25.30 35.29 Comparative Example 3 48.76 22.59 42.40 Comparative Example 4 45.43 21.24 39.72 Comparative Example 5 49.86 28.62 43.65 Comparative Example 6 53.52 31.58 46.79 Comparative Example 7 51.39 30.71 45.63 As shown in Table 1, and combining the data from Examples 1-5 and Comparative Examples 6-7, when the mass ratio of bisabolol, ergothioneine, Gynostemma pentaphyllum leaf extract, alder fruit extract, and mussel extract in the composition is (1-3):(0.2-1):(3-8):(2-5):(0.5-2), the composition exhibits optimal levels of COL1 gene upregulation, SIRT1 gene upregulation, and MMP-1 gene expression inhibition rate. This indicates that the composition demonstrates better inhibition of collagen degradation, promotion of collagen regeneration, and cellular antioxidant and anti-aging capabilities. However, when the mass ratio of bisabolol, ergothioneine, Gynostemma pentaphyllum leaf extract, alder fruit extract, and mussel extract in the composition is not (1-5):(0.1-2):(2-10):(1-6):(0.1-2), the composition's effects on inhibiting collagen degradation, promoting collagen regeneration, and activating cellular anti-aging genes are significantly reduced.

[0056] Based on the data from Example 1 and Comparative Examples 1-5, it can be seen that the upregulation rates of COL1 and SIRT1 genes and the inhibition rate of MMP-1 gene expression in Comparative Examples 1-5 were significantly lower than those in Example 1. This indicates that the bisabolol, ergothioneine, Gynostemma pentaphyllum leaf extract, alder fruit extract, and mussel extract in the composition can synergistically and effectively improve gingival collagen metabolism and inhibit cellular aging through cell anti-aging, inflammation suppression, collagen degradation blocking, and collagen promotion pathways, thereby alleviating problems such as gingival recession, loosening, and sensitive aging from the root cause.

[0057] In summary, the composition of the present invention comprises five components: bisabolol, ergothioneine, gynostemma pentaphyllum leaf extract, alder fruit extract, and mussel extract. These components synergistically achieve antioxidant and anti-inflammatory effects, creating a favorable microenvironment for gingival tissue repair. They also effectively inhibit collagen degradation and promote matrix regeneration, helping to improve gingival recession and loss of resilience. Thus, it achieves highly efficient, gentle, and long-lasting anti-gingival aging effects, and has long-lasting oral mucosal retention capacity, making it widely compatible with various oral care products such as toothpaste, mouthwash, and whitening strips.

[0058] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition for combating gingival aging, characterized in that, It includes the following components: bisabolol, ergothioneine, Gynostemma pentaphyllum leaf extract, alder fruit extract and mussel extract; wherein the mass ratio of the bisabolol, ergothioneine, Gynostemma pentaphyllum leaf extract, alder fruit extract and mussel extract is (1-5):(0.1-2):(2-10):(1-6):(0.1-2).

2. The anti-gingival aging composition according to claim 1, characterized in that, The mass ratio of bisabolol, ergothioneine, gynostemma pentaphyllum leaf extract, alder fruit extract and mussel extract is (1-3):(0.2-1):(3-8):(2-5):(0.5-2).

3. The anti-gingival aging composition according to claim 1, characterized in that, The mass ratio of bisabolol, ergothioneine, gynostemma pentaphyllum leaf extract, alder fruit extract, and mussel extract is 2:0.5:5:3:

1.

4. The use of the anti-gingival aging composition according to any one of claims 1-3 in the preparation of oral products.

5. The application as described in claim 4, characterized in that, The oral care products include toothpaste, tooth powder, mouthwash, oral spray, and teeth whitening strips; the composition for combating gingival aging is added at a mass ratio of 0.01-5%.

6. A toothpaste for combating gum aging, characterized in that, It comprises the following ingredients by weight percentage: 0.01-1% of the anti-gingival aging composition according to any one of claims 1-3, 25-35% abrasive, 20-25% humectant, 12-20% surfactant, 1-2.5% flavoring agent, 0.5-1% thickener, 0.005-0.01% sweetener, and the balance being water.

7. The toothpaste for anti-gingival aging as described in claim 6, characterized in that, The raw material is selected from at least one of (I)-(VI): (I) The friction agent is silicon dioxide; (II) The moisturizer is sorbitol and / or propylene glycol; (III) The surfactant is at least one of sodium lauroyl sarcosinate, cocamidopropyl betaine, and sodium dodecyl sulfate; (IV) The flavoring agent is a fragrance; (V) The thickener is at least one of sodium carboxymethyl cellulose, xanthan gum, and guar gum; (VI) The sweetener is at least one of sucralose, xylitol and erythritol.