Organ-like culture and drug sensitivity detection device

Abstract

The utility model belongs to the field of medical instruments, and relates to an organ-like culture and drug sensitivity detection device. The device comprises a fluid circulation unit, an imaging unit and a drug sensitive release unit, the device is of a four-side box body structure, and a cabin door is arranged on one side face perpendicular to the bottom face of a box body. The cabin door is connected with a placing platform parallel to the bottom surface of the box body towards the inside of the box body; the bottom surface or the dead center area of the bottom surface of the placing platform is of a transparent structure and is used for placing an organoid culture chip; the imaging unit is arranged in a space between the bottom surface in the box body and the placing platform; the fluid circulating unit is arranged in a space between the top in the box body and the placing platform; the placing platform is used for placing an organoid culture chip. Based on the integrated organoid culture and drug sensitivity detection device disclosed by the utility model, integrated operation of organoid culture and detection is realized, and rapid and accurate detection is realized during the culture period and after a drug sensitivity test.

Description

Technical Field

[0001] The utility model relates to the technical field of medical instruments, and in particular to an organoid culture and drug sensitivity detection device. Background Art

[0002] Organoids are millimeter- or sub-millimeter-scale miniature three-dimensional organs constructed in vitro from adult stem cells or pluripotent stem cells. The three-dimensional organoids constructed in vitro can not only reconstruct the three-dimensional spatial structure of organs in real tissues, but also construct cell lineage characteristics and microenvironmental factors. Early organoids were mainly single endothelial organ structures constructed from stem cells. With the development of biotechnology and the integration of multidisciplinary technologies, the current organoid technology is gradually building complex, multi-tissue, and highly controllable miniature organ systems. In clinical application scenarios, organoid technology can provide personalized disease models for patients with diseases such as tumors. By sampling cells from the patient's lesions, an organoid model with the same genetic background as the patient is constructed in vitro. The organoid model after in vitro expansion can replace the patient to test the therapeutic effects of different drugs, and select the best drug for the treatment of the patient. In the field of drug research and development, organoids can provide disease models with higher tissue complexity than two-dimensional cells and better genetic background similarity than experimental animals, which can be used to screen drugs and improve the safety and success rate of drug research and development. In the field of regenerative medicine, humanized organoid technology, after further development, is expected to truly construct humanized tissue and organ substitutes, thereby solving the global shortage of donated organs.

[0003] Although organoid research has brought huge opportunities for personalized medicine, drug development, and regenerative medicine, the application of organoid technology still has certain limitations in terms of technical level. On the culture and production side, traditional organoid culture utilizes the self-renewal function of stem cells, and the self-renewal, growth, and differentiation of individual organoids cannot be controlled. Organoids from the same sample source may also vary greatly. Technical errors will result in poor repeatability in subsequent drug sensitivity testing and regenerative medicine applications, hindering their further industrial transformation. Due to the complex three-dimensional structure of organoids, a wide variety of cell types, and hydrogel encapsulation, it is difficult to use traditional detection methods to achieve high-throughput organoid detection, thereby obtaining accurate organoid drug sensitivity detection information. Utility Model Content

[0004] In view of the above defects or improvement needs of the prior art, the present invention provides an organoid culture and drug sensitivity detection device. The present invention connects a placement platform to the box cabin of the device, and integrates a fluid circulation unit, an imaging unit, and a drug sensitivity release unit in the upper and lower spaces of the placement platform, thereby designing an integrated organoid culture and drug sensitivity detection device to solve the problem of culture microenvironment fluid control during organoid culture, as well as the problem of rapid and accurate detection during culture and after drug sensitivity testing.

[0005] To achieve the above-mentioned purpose, according to the first aspect of the utility model, there is provided an organoid culture and drug sensitivity detection device, the device is a four-sided box structure, and a hatch is arranged on a side perpendicular to the bottom surface of the box; the hatch is connected to a placement platform parallel to the bottom surface of the box toward the inside of the box; the bottom surface of the placement platform or the center area of ​​the bottom surface is a transparent structure for placing an organoid culture chip;

[0006] The device includes a fluid circulation unit, an imaging unit and a drug-sensitive release unit arranged in the box body; wherein the imaging unit is arranged in the space between the bottom surface of the box body and the placement platform; the fluid circulation unit is arranged in the space between the top of the box body and the placement platform; wherein the drug-sensitive release unit includes an online pressing flat plate device for pressing the drug-sensitive unit in the organoid culture chip to release the drug.

[0007] Furthermore, the fluid circulation unit comprises a liquid pump and a docking pipe, and the liquid pump is connected to the docking pipe to form a fluid access passage and a fluid outflow passage, respectively, for performing fluid circulation on the organoid culture chip.

[0008] Furthermore, the device also includes a three-axis translation platform, which is arranged on the bottom surface of the box body and is used to translate the imaging unit to a preset position below the organoid culture chip and adjust the imaging position of the organoid culture chip.

[0009] Furthermore, the imaging unit includes an optical path element, an objective lens, a reflector, a light source and a camera; wherein the light source is a visible light or a fluorescent excitation light source.

[0010] The objective lens is arranged below the placement platform, the reflector and the camera are arranged on the same straight line, and the angle formed by the objective lens and the mirror surface of the reflector and the angle formed by the mirror surface of the reflector and the camera are both 45 degrees, so that the image of the organoid culture chip reaches the reflector through the objective lens and is reflected to the camera.

[0011] Furthermore, the device also includes a temperature control unit, a carbon dioxide maintenance unit and a sterilization unit, all of which are arranged in the box.

[0012] Furthermore, the temperature control unit includes a temperature sensor and an electric heating device; the electric heating device is used to adjust the temperature inside the box based on the temperature parameter of the temperature sensor.

[0013] Furthermore, the carbon dioxide maintenance unit includes a carbon dioxide concentration detector, a carbon dioxide inlet and an inlet valve; the inlet valve is connected to the carbon dioxide inlet and is used to adjust the switch of the inlet valve based on the carbon dioxide concentration of the carbon dioxide concentration detector to control the amount of carbon dioxide flowing into the carbon dioxide inlet.

[0014] Furthermore, the sterilization unit includes an ultraviolet lamp for disinfecting the environment inside the box.

[0015] Furthermore, the device also includes a device operation integrated unit arranged outside the box; the device operation integrated unit is connected to other units of the device for performing integrated operation on the device.

[0016] In general, the above technical solution conceived by the utility model has the following technical advantages compared with the prior art:

[0017] (1) Compared with traditional equipment, in the device of the utility model, an integrated organoid culture and drug sensitivity detection device is designed by connecting a placement platform to the box cabin of the device, and integrating a fluid circulation unit, an imaging unit and a drug sensitivity release unit in the upper and lower spaces of the placement platform. At the same time, the fluid circulation unit of the device can realize the cyclic inoculation and fluid replacement of organoids. Based on the fluid circulation unit, the culture fluid of the organoid can be replaced, and it can also be used to change the liquid microenvironment in the organoid. By regulating the flow rate, the fluid shear force in the organoid environment can also be changed. Based on the above, the device can realize the controllable culture microenvironment fluid during organoid culture, and realize the integrated and precise operation of the device during the culture period and after the drug sensitivity test.

[0018] (2) The real-time imaging part and the organoid culture part of the utility model are respectively placed on the upper and lower sides of the organoid chip without interfering with each other. Preferably, the imaging unit in the device can realize dual-modal imaging of bright field or fluorescence field, and the imaging system can be programmed to move in the xyz axis to realize long-term real-time imaging of the organoid. Compared with the traditional separate setting of organoid culture equipment and detection equipment, real-time detection in organoid culture and drug testing can be realized, the status of organoids can be tracked in real time, and the whole process can be integrated.

[0019] (3) The device integrates the trigger elements of drug administration and drug sensitivity testing of organoids. By pressing the linear controller on the organoid chip, the pre-embedded drug in the chip can be released. After the drug release is completed, the above-mentioned imaging system can be used to monitor the response of the organoid after drug treatment, thereby completing the organoid drug sensitivity test.

[0020] (4) Preferably, the device is also provided with a temperature control unit, a carbon dioxide maintenance unit and a sterilization unit in the box body to control the temperature and carbon dioxide in the in vitro culture environment of the organoid, so as to set and adjust the culture environment of the organoid.

[0021] (5) Preferably, in the present invention, precise control of each unit in the device is accomplished through the device operation integrated unit, for example, fluid flow rate and flow rate are controlled by controlling the flow circulation unit, drug release is controlled by the drug-sensitive release unit, real-time imaging acquisition is achieved by the imaging unit, and the culture and detection environment are adjusted by the temperature control unit, the carbon dioxide maintenance unit and the sterilization unit, etc., thereby accurately realizing the integrated operation of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the organoid culture and drug sensitivity detection device;

[0023] Figure 2 It is a schematic diagram of the organoid culture chip of the organoid culture and drug sensitivity detection device;

[0024] Figure 3 It is a schematic diagram of the liquid pump in the organoid culture and drug sensitivity detection device;

[0025] Figure 4 It is a schematic diagram of the imaging system of the organoid culture and drug sensitivity detection device;

[0026] Figure 5 It is a schematic diagram of the interface between the pressing plate device and the liquid pump on the midline of the organoid culture and drug sensitivity detection device;

[0027] Figure 6 This is a schematic diagram of the sterilization unit in the organoid culture and drug sensitivity testing device.

[0028] In all the drawings, the same reference numerals are used to represent the same elements or structures, wherein: 1-box chamber, 2-imaging optical path, 3-display screen, 4-fluid pump, 5-organoid culture chip, 6-online pressing flat plate device, 7-placing platform, 8-cabinet door, 9-three-axis translation platform, 10-auxiliary optical path, 11-reflector, 12-objective lens, 13-visible light source, 14-sterilization unit, 15-organoid culture chip interface, 16-camera. DETAILED DESCRIPTION

[0029] In order to make the purpose, technical solution and advantages of the utility model more clear, the utility model is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the utility model and are not used to limit the utility model. In addition, the technical features involved in each embodiment of the utility model described below can be combined with each other as long as they do not conflict with each other.

[0030] The organoid culture and drug sensitivity detection device provided by the utility model is as follows: Figure 1 As shown, the device of the present invention is a detachable four-sided box structure, and a hatch 8 is provided on any side perpendicular to the bottom surface. The hatch is connected to the inside of the box and is parallel to the bottom surface of the box.

[0031] The hatch is a pop-up hatch for use with different types of organoid chips. The bottom surface or the center area of ​​the bottom surface of the placement platform is a transparent structure for placing the organoid culture chip.

[0032] The box of the device of the utility model integrates a fluid circulation unit, an imaging unit and a drug-sensitive release unit to form an integrated organoid culture and detection device.

[0033] The imaging unit is arranged in the space between the bottom surface of the box and the organoid chip placement platform; the organoid culture chip (such as Figure 2 The organoid culture chip shown in the utility model is placed on a transparent area on the placement platform; the fluid circulation unit is arranged in the space between the top of the box body and the organoid chip placement platform; the fluid circulation unit is used to control the flow rate of the culture solution or other liquid in the organoid culture chip, and the imaging unit performs corresponding bright field imaging or fluorescence imaging on the organoid culture chip. The bottom surface or the center area of ​​the bottom surface of the placement platform is a transparent structure, i.e., an imaging window, which facilitates the imaging unit below the imaging window to image the organoid culture chip.

[0034] The fluid circulation unit is used to control the flow rate and flow rate of the culture solution containing organoid cells or the culture solution without organoid cells in the corresponding components.

[0035] Among them, the drug sensitivity release unit is arranged in the box, including an online pressing flat plate device, which is used to press the drug sensitivity unit in the organoid culture chip through the online pressing flat plate device, thereby releasing the drug sensitivity structure (drug) inside the chip to realize the drug sensitivity test of the organoid.

[0036] Furthermore, the fluid circulation unit includes a liquid pump (such as Figure 3As shown) and a docking pipeline, a liquid pump is connected to the docking pipeline to form a fluid access passage and a fluid outflow passage, respectively, for fluid circulation of the organoid culture chip.

[0037] The end of the fluid pump pipeline is provided with an organoid culture chip interface with an inlet and outlet interface that can be docked with the organoid culture chip. The interface is consistent with the inlet and outlet interface of the organoid chip, and can be docked by a motor after the organoid chip is sampled. That is, the fluid pump is connected to the organoid culture chip interface through the docking pipeline, so that the culture fluid in the organoid culture environment is driven to flow by the liquid pump.

[0038] Among them, the liquid pump can be a peristaltic pump, a pressure pump, a syringe pump, etc.

[0039] Furthermore, the device also includes a three-axis translation platform, which is arranged on the bottom surface of the device box, and is used to translate the imaging unit to a preset bottom of the organoid culture chip through the three-axis translation platform, and adjust the imaging position of the organoid culture chip.

[0040] Furthermore, the imaging unit includes an optical path element, an objective lens, a reflector, a visible light or fluorescent excitation light source, and a camera. The objective lens is arranged below the placement platform, the reflector and the camera are arranged on the same straight line, and the angle formed by the objective lens and the mirror surface of the reflector and the angle formed by the mirror surface of the reflector and the camera are both 45 degrees, so that the image of the organoid culture chip reaches the reflector through the objective lens and is reflected to the camera. The optical path of the visible light or fluorescent excitation light source is switched by the optical path element, and the camera for shooting is used to record and take pictures.

[0041] During organoid culture, the imaging unit can record the organoid culture conditions without changing the organoid culture conditions including temperature, carbon dioxide concentration and the microenvironment around the organoids, so as to determine whether to change the culture conditions. After the drug sensitivity test, the organoid culture chip can be imaged by fluorescence to detect the drug sensitivity effect.

[0042] Furthermore, the device operation integrated unit is connected to the above-mentioned fluid circulation unit, imaging unit and drug-sensitive release unit, and is arranged outside the box to control the organoid culture and detection in the box to realize real-time and accurate integrated culture and detection operations.

[0043] The fluid pump in the fluid circulation unit is connected to the above-mentioned device operation integrated unit, so that the flow rate and flow rate of the fluid pump can be adjusted through software or the device itself.

[0044] The imaging unit is connected to the above-mentioned device operation integrated unit, and cyclic imaging recording is achieved through the setting of the device operation integrated unit.

[0045] As an implementation mode of the present utility model, Figure 4 As shown, the imaging optical path 2 is built on a three-axis translation platform 9. The imaging optical path 2 includes an auxiliary optical path 10 capable of dual-mode switching, a camera 16, a reflector 11, an objective lens 12, and a visible light source 13.

[0046] The dual-mode switching auxiliary optical path 10 is on the same plane as the imaging optical path 2 and is perpendicular to the imaging optical path 2. The fluorescent light and the visible light are switched by switching the optical paths.

[0047] The front end of the imaging optical path 2 is connected to the reflector 11 , and the rear end is connected to the camera 16 . The light reflected by the reflector 11 passes through the imaging optical path 2 and is photographed by the camera 16 to form an image.

[0048] The front end of the imaging optical path 2 is connected to the open objective lens 12 through a 45-degree reflector 11. The objective lens 12 is located directly above the reflector and focuses on the imaging area of ​​the organoid culture chip 5. Light sources 13 are set on both sides of the objective lens 12.

[0049] The objective lens is arranged below the placement platform, the reflector and the camera are arranged on the same straight line, and the angle formed by the objective lens and the mirror surface of the reflector and the angle formed by the mirror surface of the reflector and the camera are both 45 degrees, so that the image of the organoid culture chip reaches the reflector through the objective lens and is reflected to the camera; the fluorescent excitation light source reaches the reflector through the auxiliary light path and the imaging light path, and is reflected through the objective lens to reach the organoid culture chip.

[0050] The three-axis translation platform is arranged on the bottom surface of the box body, and the three-axis translation platform is arranged to translate the unit in the imaging unit to the bottom of the organoid culture chip on the xy-axis plane through the device operation integrated unit, and the objective lens position is adjusted on the z-axis to achieve the best imaging position. The above components are moved on the organoid culture chip through the three-axis translation platform 9 through the motor to control the imaging range so that the required imaging position can be clearly photographed.

[0051] Furthermore, the drug sensitivity release unit online pressing plate device (i.e., drug sensitivity pressing unit) of the utility model is a plate of a size corresponding to the drug sensitivity release equipment in the organoid culture chip, located directly above the position of the organoid culture chip in the box, and is used for releasing drugs from the drug sensitivity structure inside the organoid chip by pressing downward during drug sensitivity testing, thereby realizing drug sensitivity testing of the organoid.

[0052] like Figure 5 As shown, the online pressing plate device 6 and the organoid culture chip interface 15. Below the online plate pressing plate device 6 is the organoid culture chip interface 15, and the two are connected together by a support and can move linearly downwards respectively.

[0053] The online pressing flat plate device 6 presses the organoid culture chip 5 in the vertical direction through a motor, and triggers the release of pre-prepared drugs in the organoid culture chip by pressing the chip, thereby triggering the drug sensitivity test of the cultured organoids. After the reaction is complete, the cell activity is continued to be read through the imaging unit to obtain the drug sensitivity test result.

[0054] Furthermore, the device of the utility model also includes a temperature control unit, a sterilization unit and a carbon dioxide maintenance unit, all of which are arranged in a box; the temperature control unit is used to maintain the temperature in the box within a preset temperature range, and the preset temperature range is the requirement for organoid culture; the sterilization unit is used to sterilize the culture environment in the box; the carbon dioxide maintenance unit is used to maintain the carbon dioxide concentration in the box within a preset concentration range, and the preset concentration range is the requirement for organoid culture.

[0055] Furthermore, the temperature control unit, the sterilization unit and the carbon dioxide maintenance unit are arranged in the box body and in a space away from the cabin door, for example, in the rear area of ​​the device.

[0056] The temperature control unit includes a temperature sensor, an electric heating device and a circulating fan, and the temperature sensor is isolated from the electric heating device at a certain distance so that the temperature collected by the temperature sensor is not affected by the electric heating device as much as possible. For example, if the temperature sensor is fixedly connected to the top of the box, the electric heating device is set on the side of the box away from the temperature sensor. Through the linkage between the temperature sensor and the electric heating device, the electric heating device controls the electric heating based on the temperature parameters of the temperature sensor to maintain or adjust the culture and detection temperature of the organoid culture chip; and the circulating fan makes the air in the box convect by running the circulating fan, so that the air temperature in the box is more uniform, and the carbon dioxide concentration in the box is more uniform.

[0057] The carbon dioxide maintenance unit includes a carbon dioxide concentration sensor, a carbon dioxide inlet and outlet, and an air intake valve. The carbon dioxide concentration sensor and the air intake valve have a linkage feedback, and the opening and closing of the air intake valve are adjusted to maintain the concentration of carbon dioxide in the box. Specifically, the air intake valve is connected to the carbon dioxide inlet, and the opening and closing of the air intake valve are adjusted based on the carbon dioxide concentration of the carbon dioxide concentration detector to control the amount of carbon dioxide flowing into the carbon dioxide inlet.

[0058] For example, the temperature carbon dioxide control unit is connected to a temperature sensor and a carbon dioxide concentration sensor, and is arranged above the box inside the box chamber. The temperature carbon dioxide unit is controlled to control the temperature and carbon dioxide through signal feedback to the device operation integrated unit. It should be noted that according to the conventional design of personnel in this field, when it comes to the interface of the carbon dioxide interface, the temperature sensor and the carbon dioxide concentration detector, it must be connected to the outside of the box through the above interface, so the part with the interface is exposed on the box shell in design.

[0059] Among them, Figure 6 As shown, a sterilization unit 14 is arranged above the chamber 1 of the organoid culture chip; the sterilization unit 14 is mainly composed of ultraviolet lamps, which are used to generate ozone in the box for sterilization by ultraviolet light.

[0060] Furthermore, the device operation integrated unit is arranged outside the box, for example, it can be fixedly connected to the top of the box, or externally connected and controlled.

[0061] For example, the device operation integrated unit can be placed in a portable computer, connected to the display screen 3 on the device box through an external computer interface opened on the device box shell of the utility model, and the supporting software integration can operate all the original parts of the device as a whole.

[0062] Furthermore, the device of the utility model contains a matching organoid culture chip interface, and a matching organoid chip compartment and a pop-up door are provided on the front side of the device housing for use with different types of organoid culture chips.

[0063] The organoid culture and drug sensitivity detection device described in the above embodiment realizes the culture and drug sensitivity detection of organoids by adapting the organoid culture chip or culture vessel, as follows:

[0064] The organoid chip or culture vessel is loaded with the organoid culture chip through the hatch at the front of the box. After the organoid culture chip enters the hatch, the organoid culture chip interface on the top of the box cavity is pressed down to dock with the sample inlet and outlet ports at both ends of the organoid culture chip to achieve control of the liquid flow inside the organoid culture chip.

[0065] After the organoid culture chip is connected to the liquid pump, turn on the electric heating plate and circulating fan at the rear of the device box cavity, as well as the temperature sensor at the top of the box cavity, to maintain a constant temperature environment in the box cavity; at the same time, turn on the carbon dioxide valve at the rear of the device box cavity and the carbon dioxide concentration sensor at the top of the box cavity, and regulate the switch of the carbon dioxide valve through feedback to maintain the stable carbon dioxide concentration in the device box cavity.

[0066] While the organoids are being cultured normally, the device turns on the imaging element at the bottom of the chamber. The element uses an optical path on a three-axis translation platform to support the entire structure. A camera is connected to the end of the three-dimensionally movable optical path. Due to the state of the organoids being imaged, a conversion device is added to the optical path to achieve dual-modal imaging of fluorescence or bright field. The left and right ends of the objective lens edge of the shooting system are respectively equipped with white light fields for bright field imaging, and the conversion device is equipped with a fluorescent light source for fluorescent imaging. The above-mentioned element is controlled to move in the chamber through a three-axis mobile platform, thereby controlling the imaging range and focus.

[0067] After the organoid culture matures, there is a pressure plate structure driven by a linear controller above the organoid culture chip inside the device box. The pressure plate structure is activated after the organoid culture matures, and the organoid chip is operated by pressing to release the pre-prepared drug on the cover above the organoid chip. After the drug is released, it triggers the organoid-based drug sensitivity detection, and the efficacy is evaluated by the above-mentioned imaging system.

[0068] The device of the present invention realizes accurate operation of the entire process through a computer that is built into the device or connected to an external computer (such as a control panel in the device operation integrated unit, etc.).

[0069] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalent technologies, the present invention is also intended to include these changes and variations. The above-described embodiments are only preferred embodiments for fully illustrating the present invention, and their protection scope is not limited thereto. Equivalent substitutions or changes made by those skilled in the art on the basis of the present invention are all within the protection scope of the present invention, and the protection scope of the present invention shall be subject to the claims.

Claims

1. An organoid culture and drug sensitivity detection device, It is characterized in that The device is a four-sided box structure, and a hatch is provided on a side perpendicular to the bottom of the box; the hatch is connected to a placement platform parallel to the bottom of the box toward the inside of the box; the bottom surface or the center area of ​​the bottom surface of the placement platform is a transparent structure for placing the organoid culture chip; The device includes a fluid circulation unit, an imaging unit and a drug sensitivity release unit arranged in the box body; wherein the imaging unit is arranged in the space between the bottom surface of the box body and the placement platform; the fluid circulation unit is arranged in the space between the top of the box body and the placement platform; wherein the drug sensitivity release unit includes an online pressing flat plate device for pressing the drug sensitivity unit in the organoid culture chip to release the drug.

2. The organoid culture and drug sensitivity detection device according to claim 1, It is characterized in that The fluid circulation unit comprises a liquid pump and a docking pipe, wherein the liquid pump is connected with the docking pipe to form a fluid access passage and a fluid outflow passage, respectively, for performing fluid circulation on the organoid culture chip.

3. The organoid culture and drug sensitivity detection device according to claim 1, It is characterized in that The device also includes a three-axis translation platform, which is arranged on the bottom surface of the box body and is used to translate the imaging unit to a preset lower part of the organoid culture chip and adjust the imaging position of the organoid culture chip.

4. The organoid culture and drug sensitivity detection device according to claim 1, It is characterized in that The imaging unit comprises an optical path element, an objective lens, a reflector, a light source and a camera; wherein the light source is a visible light or a fluorescent excitation light source; The objective lens is arranged below the placement platform, the reflector and the camera are arranged on the same straight line, and the angle formed by the objective lens and the mirror surface of the reflector and the angle formed by the mirror surface of the reflector and the camera are both 45 degrees, so that the image of the organoid culture chip reaches the reflector through the objective lens and is reflected to the camera.

5. The organoid culture and drug sensitivity detection device according to claim 1, It is characterized in that The device also includes a temperature control unit, a carbon dioxide maintenance unit and a sterilization unit, all of which are arranged in the box.

6. The organoid culture and drug sensitivity detection device according to claim 5, It is characterized in that The temperature control unit includes a temperature sensor and an electric heating device; the electric heating device is used to adjust the temperature inside the box based on the temperature parameter of the temperature sensor.

7. The organoid culture and drug sensitivity detection device according to claim 5, It is characterized in that The carbon dioxide maintenance unit includes a carbon dioxide concentration detector, a carbon dioxide air inlet and an air inlet valve; the air inlet valve is connected to the carbon dioxide air inlet and is used to adjust the switch of the air inlet valve based on the carbon dioxide concentration of the carbon dioxide concentration detector to control the amount of carbon dioxide flowing into the carbon dioxide air inlet.

8. The organoid culture and drug sensitivity detection device according to claim 5, It is characterized in that The sterilization unit comprises an ultraviolet lamp for sterilizing the environment within the box.

9. The organoid culture and drug sensitivity detection device according to any one of claims 1 to 8, It is characterized in that The device further comprises a device operation integrated unit arranged outside the box; the device operation integrated unit is connected with other units of the device and is used for performing integrated operation on the device.

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