Chlorophyll concentration detection device

Abstract

The utility model relates to the technical field of chlorophyll concentration detection, in particular to a chlorophyll concentration detection device which comprises an operation table, one side of the top of the operation table is fixedly connected with a cuvette frame used for detecting concentration, an inner cavity of the cuvette frame is fixedly connected with a dish body, the surface of the cuvette frame is fixedly connected with a fixing plate, and the fixing plate is fixedly connected with the cuvette frame. By arranging a reflecting plate, when concentration detection is carried out, firstly, the motor rotates to drive a rotating rod to rotate, the rotating rod rotates to drive a circular plate to rotate, the circular plate rotates to drive a connecting rod to move, and the connecting rod moves to drive the reflecting plate to carry out angle adjustment; according to the chlorophyll culture dish, the reflected red light can be secondarily reflected and re-emitted into the dish body, so that the chlorophyll fully absorbs the red light, the chlorophyll concentration can be more accurately detected, the red light can be fully secondarily reflected through multi-angle adjustment, and the amount of the chlorophyll absorbing the red light is increased.

Description

Technical Field

[0001] The utility model relates to the technical field of chlorophyll concentration detection, in particular to a chlorophyll concentration detection device. Background Art

[0002] Chlorophyll is the main pigment for photosynthesis in green plants. The determination of chlorophyll content is widely used in many fields such as the study of photosynthesis, diagnosis and monitoring of plant nutritional status, and the ripening mechanism of fruits and vegetables. However, these applications require the determination of the chlorophyll content in green plants. The chlorophyll extract obtained by extraction mainly contains pigments such as chlorophyll and carotenoids. Chlorophyll mainly absorbs red light and blue-violet light, and carotenoids mainly absorb blue-violet light. The chlorophyll content in the chlorophyll extract can be determined by detecting the red light absorption of chlorophyll.

[0003] However, when monochromatic light is irradiated to the solution, the solution is a uniform medium, part of the light beam will be absorbed by the solution, and part of the light beam will be reflected or scattered due to problems such as the vessel. In this way, the red light absorbed by chlorophyll is incomplete, reducing the accuracy of the experiment. Utility Model Content

[0004] Technical issues solved

[0005] In view of the above-mentioned shortcomings of the prior art, the utility model provides a chlorophyll concentration detection device, which can effectively solve the problem in the prior art that part of the light beam will be absorbed by the solution, and part of the light beam will be reflected or scattered due to problems such as utensils, so that the red light absorbed by chlorophyll is incomplete, reducing the accuracy of the experiment.

[0006] Technical Solution

[0007] In order to achieve the above objectives, the present invention is implemented through the following technical solutions:

[0008] The utility model provides a chlorophyll concentration detection device, comprising an operating table, a cuvette holder for detecting concentration is fixedly connected to one side of the top of the operating table, a cuvette body is fixedly connected to the inner cavity of the cuvette holder, a fixing plate is fixedly connected to the surface of the cuvette holder, a motor is fixedly connected to the top of the fixing plate, a rotating rod is fixedly connected to the output end of the motor, a circular plate is fixedly connected to the end of the rotating rod away from the motor, a first rotating shaft is rotatably connected to one side of the circular plate, a connecting rod is fixedly connected to the surface of the first rotating shaft, and the circular plate is fixedly connected to the end of the connecting rod away from the first rotating shaft; and also comprising a reflecting plate, the reflecting plate is used to reflect red light.

[0009] Furthermore, the top of the cuvette holder is fixedly connected to a vertical rod, the top of the vertical rod is fixedly connected to a horizontal rod, one end of the horizontal rod is rotatably connected to a movable shaft, the surface of the movable shaft is fixedly connected to a docking block, and one side of the docking block is fixedly connected to the back of the reflector.

[0010] Furthermore, one side of the reflective plate is rotatably connected to a second rotating shaft, and one end of the second rotating shaft is rotatably connected to one side of the circular plate.

[0011] Furthermore, the pair of reflective plates are provided in a total of several groups, and the reflective plates in the groups are distributed above the cuvette holder in a circular array, and the reflective plates are arranged obliquely.

[0012] Furthermore, a rotating plate is movably connected to the top of the operating table, a support rod is fixedly connected to the top of the rotating plate, a truncated table is fixedly connected to the top of the support rod, a connecting arm is fixedly connected to one side of the truncated table, a connecting arm is fixedly connected to the bottom of the connecting arm away from the end of the truncated table, a mounting plate is fixedly connected to the bottom of the connecting rod, a main tungsten lamp is fixedly connected to the middle position of the bottom of the mounting plate, and a secondary tungsten lamp is fixedly connected to the edge position of the bottom of the mounting plate.

[0013] Furthermore, the auxiliary tungsten lamps are provided in a plurality of groups, and the plurality of groups of auxiliary tungsten lamps are distributed in a circular array at the bottom of the mounting plate.

[0014] Beneficial Effects

[0015] Compared with the known public technology, the technical solution provided by the utility model has the following beneficial effects:

[0016] Beneficial effect 1: By setting up a reflective plate, when conducting concentration detection, first the motor rotates to drive the rotating rod to rotate, the rotating rod rotates to drive the circular plate to rotate, the circular plate rotates to drive the connecting rod to move, and the movement of the connecting rod drives the reflective plate to adjust the angle, so that the reflected red light can be reflected for a second time and re-injected into the dish body, so that the chlorophyll can fully absorb the red light, and the chlorophyll concentration can be detected more accurately. Multi-angle adjustment can fully reflect the red light for a second time and increase the amount of red light absorbed by chlorophyll.

[0017] Beneficial effect 2: The main tungsten lamp and the auxiliary tungsten lamp are set to provide sufficient lighting. The light source is a tungsten lamp, which is an electric light source made by adding a trace of halogen or halide to the inert gas filled in the incandescent lamp. It has the advantages of small size, high luminous efficiency, stable color temperature, almost no light decay, and long life. Tungsten lamps are also commonly used light sources in existing visible light spectrophotometers. Setting multiple groups of tungsten lamps makes the lighting more sufficient. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the prior art descriptions are briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0019] Figure 1 It is the front view of the utility model;

[0020] Figure 2 It is a three-dimensional diagram of the cuvette holder of the utility model;

[0021] Figure 3 A three-dimensional diagram of the reflector of the present invention;

[0022] Figure 4 It is a three-dimensional diagram of the main tungsten lamp of the present utility model.

[0023] The numbers in the figure represent: 1. operating table; 2. cuvette holder; 3. fixing plate; 4. motor; 5. rotating rod; 6. circular plate; 7. first rotating axis; 8. connecting rod; 9. circular plate; 10. vertical rod; 11. horizontal rod; 12. movable axis; 13. docking block; 14. reflecting plate; 15. second rotating axis; 16. rotating plate; 17. supporting rod; 18. round table; 19. connecting arm; 20. connecting rod; 21. mounting plate; 22. main tungsten lamp; 23. auxiliary tungsten lamp; 24. cuvette body. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical solution and advantages of the embodiment of the utility model clearer, the technical solution in the embodiment of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiment of the utility model. Obviously, the described embodiment is a part of the embodiment of the utility model, not all of the embodiments. Based on the embodiment of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0025] The utility model is further described below in conjunction with embodiments.

[0026] See also Figures 1 to 4A chlorophyll concentration detection device comprises an operating table 1, a cuvette holder 2 for detecting concentration is fixedly connected to one side of the top of the operating table 1, a cuvette body 24 is fixedly connected to the inner cavity of the cuvette holder 2, a fixed plate 3 is fixedly connected to the surface of the cuvette holder 2, a motor 4 is fixedly connected to the top of the fixed plate 3, a rotating rod 5 is fixedly connected to the output end of the motor 4, a circular plate 6 is fixedly connected to the end of the rotating rod 5 away from the motor 4, a first rotating shaft 7 is rotatably connected to one side of the circular plate 6, a connecting rod 8 is fixedly connected to the surface of the first rotating shaft 7, and a circular plate 9 is fixedly connected to the end of the connecting rod 8 away from the first rotating shaft 7; and also comprises; The reflecting plate 14 is used for reflecting the red light. The top of the cuvette holder 2 is fixedly connected with a vertical rod 10, the top of the vertical rod 10 is fixedly connected with a horizontal rod 11, one end of the horizontal rod 11 is rotatably connected with a movable shaft 12, the surface of the movable shaft 12 is fixedly connected with a docking block 13, one side of the docking block 13 is fixedly connected to the back of the reflecting plate 14, one side of the reflecting plate 14 is rotatably connected with a second rotating shaft 15, one end of the second rotating shaft 15 is rotatably connected to one side of the circular plate 9, and a total of several groups of reflecting plates 14 are arranged, and the several groups of reflecting plates 14 are distributed in a circular array above the cuvette holder 2, and the reflecting plates 14 are arranged obliquely.

[0027] The motor 4 rotates to drive the rotating rod 5 to rotate, the rotating rod 5 rotates to drive the circular plate 6 to rotate, the circular plate 6 rotates to drive the connecting rod 8 to move, the connecting rod 8 moves to drive the reflecting plate 14 to adjust the angle, so that the reflected red light can be reflected for a second time and re-injected into the dish body, so that the chlorophyll can fully absorb the red light, and the chlorophyll concentration can be detected more accurately. Multi-angle adjustment can fully reflect the red light for a second time and increase the amount of red light absorbed by chlorophyll.

[0028] A rotating plate 16 is movably connected to the top of the operating table 1, a supporting rod 17 is fixedly connected to the top of the rotating plate 16, a truncated table 18 is fixedly connected to the top of the supporting rod 17, a connecting arm 19 is fixedly connected to one side of the truncated table 18, a connecting rod 20 is fixedly connected to the bottom of the connecting arm 19 away from the truncated table 18, a mounting plate 21 is fixedly connected to the bottom of the connecting rod 20, a main tungsten lamp 22 is fixedly connected to the middle position of the bottom of the mounting plate 21, an auxiliary tungsten lamp 23 is fixedly connected to the edge position of the bottom of the mounting plate 21, and a total of several groups of auxiliary tungsten lamps 23 are arranged, and the groups of auxiliary tungsten lamps 23 are distributed in a circular array at the bottom of the mounting plate 21.

[0029] The light source is a tungsten lamp. Tungsten lamps are electric light sources made by adding trace amounts of halogen or halide to the inert gas filled in incandescent lamps. They have the advantages of small size, high luminous efficiency, stable color temperature, almost no light decay, and long life. Tungsten lamps are also commonly used light sources in existing visible light spectrophotometers. Multiple groups of tungsten lamps are set to make the lighting more sufficient.

[0030] Working principle: According to the investigation, the chlorophyll extract mainly includes chlorophyll and carotenoids. The wavelength range of the normal light absorption value of chlorophyll is 400 to 780nm, and it mainly absorbs blue-violet light and red light, while carotenoids mainly absorb blue-violet light. If you want to determine the content of chlorophyll in the extract, you need to use the characteristic of chlorophyll absorbing red light. The absorption peak of chlorophyll is at 645nm and 663nm, and the wavelength of the light emitted by the tungsten lamp is 380nm to 780nm. Use a focusing mirror to focus the light, and use a collimator to make the light vertically enter the filter. The filter only allows red light with a wavelength of 600 to 700nm to pass, so that a red light source can be obtained.

[0031] When monochromatic light is irradiated to a solution, the solution is a uniform medium. If scattering is not considered, part of the light beam will be absorbed by the solution, part of the light beam will be transmitted through the solution, and part of the light beam will be reflected through the container through the solution. Since the measurements are all made using sample cells of uniform specifications, their thickness and material are the same, so the reflected light intensity can be ignored during the measurement process. The ratio of the transmitted light intensity to the incident light intensity is called transmittance, and the ratio of the absorbed light intensity to the incident light intensity is called absorbance. The greater the transmitted light intensity, the weaker the absorption effect of the solution. The strength of the absorption effect of the solution is related to factors such as the incident wavelength of the incident light, the length of the solution through which the incident light passes, and the concentration of the solution.

[0032] In the experiment, standard solution is first needed for calibration. Green plant samples can be extracted with 80% acetone solution or 95% ethanol solution. Since the maximum absorption peak of chlorophyll in acetone solution is at 663 and 645nm, and the maximum absorption peak of chlorophyll in ethanol solution is at 665 and 649nm, the calibration curves of chlorophyll extracts obtained with different extracts are different. One of the conditions for the establishment of Beer-Lambert's law is that the test object is a uniform dilute solution, and the concentration of chlorophyll extract needs to be diluted twice. After obtaining the fitting curve, put the test solution into the dish body 24, and the chlorophyll concentration can be displayed.

[0033] When detecting the chlorophyll concentration, firstly put the test object into the dish body 24, then rotate the rotating plate 16, and stop when the mounting plate 21 is rotated to the top of the dish body 24, and the main tungsten lamp 22 and the auxiliary tungsten lamp 23 are used for illumination. At this time, the rotating rod 5 is driven to rotate by the rotation of the motor 4, and the rotating rod 5 is driven to rotate by the rotation of the circular plate 6. The rotation of the circular plate 6 drives the connecting rod 8 to move, and the movement of the connecting rod 8 drives the reflecting plate 14 to adjust the angle, so that the reflected red light can be reflected twice, and the chlorophyll concentration of the dish body 24 at different angles can be detected to obtain accurate data.

[0034] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some of the technical features therein by equivalents. These modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the protection scope of the technical solutions of the embodiments of the present invention.

Claims

1. A chlorophyll concentration detection device, comprising an operating table (1), It is characterized in that A cuvette holder (2) for detecting concentration is fixedly connected to one side of the top of the operating table (1); a cuvette body (24) is fixedly connected to the inner cavity of the cuvette holder (2); a fixing plate (3) is fixedly connected to the surface of the cuvette holder (2); a motor (4) is fixedly connected to the top of the fixing plate (3); a rotating rod (5) is fixedly connected to the output end of the motor (4); a circular plate (6) is fixedly connected to the end of the rotating rod (5) away from the motor (4); a first rotating shaft (7) is rotatably connected to one side of the circular plate (6); a connecting rod (8) is fixedly connected to the surface of the first rotating shaft (7); and a circular plate (9) is fixedly connected to the end of the connecting rod (8) away from the first rotating shaft (7); Also includes; A reflective plate (14), wherein the reflective plate (14) is used to reflect red light.

2. A chlorophyll concentration detection device according to claim 1, It is characterized in that The top of the cuvette holder (2) is fixedly connected to a vertical rod (10), the top of the vertical rod (10) is fixedly connected to a horizontal rod (11), one end of the horizontal rod (11) is rotatably connected to a movable shaft (12), a surface of the movable shaft (12) is fixedly connected to a docking block (13), and one side of the docking block (13) is fixedly connected to the back of a reflective plate (14).

3. A chlorophyll concentration detection device according to claim 2, It is characterized in that One side of the reflection plate (14) is rotatably connected to a second rotation shaft (15), and one end of the second rotation shaft (15) is rotatably connected to one side of the circular plate (9).

4. A chlorophyll concentration detection device according to claim 1, It is characterized in that A total of several groups of the reflection plates (14) are provided, and the groups of the reflection plates (14) are distributed above the cuvette holder (2) in a circular array, and the reflection plates (14) are arranged at an angle.

5. A chlorophyll concentration detection device according to claim 1, It is characterized in that The top of the operating table (1) is movably connected to a rotating plate (16), the top of the rotating plate (16) is fixedly connected to a support rod (17), the top of the support rod (17) is fixedly connected to a round table (18), one side of the round table (18) is fixedly connected to a connecting arm (19), the bottom of the connecting arm (19) away from the round table (18) is fixedly connected to a connecting rod (20), the bottom of the connecting rod (20) is fixedly connected to a mounting plate (21), the middle position of the bottom of the mounting plate (21) is fixedly connected to a main tungsten lamp (22), and the edge position of the bottom of the mounting plate (21) is fixedly connected to an auxiliary tungsten lamp (23).

6. A chlorophyll concentration detection device according to claim 5, It is characterized in that A total of several groups of auxiliary tungsten lamps (23) are provided, and the groups of auxiliary tungsten lamps (23) are distributed in a circular array at the bottom of the mounting plate (21).

Images