Bioreactor capable of realizing large-scale adherent culture of microalgae

Abstract

The utility model discloses a bioreactor capable of realizing large-scale adherent culture of microalgae, which belongs to the technical field of microalgae culture equipment and comprises a support system, an adherent culture unit and a culture medium circulating module. The culture medium circulating module comprises a circulating water tank, a circulating pipeline and a liquid supplementing branch pipe which are communicated in sequence; the support system comprises a main support and support branches connected to the two opposite sides of the main support at equal intervals, each support branch is covered with a supporting body, the culture carrier is hung on the supporting bodies, and a fixing clamp used for enabling the two half parts of the culture carrier to be attached together is arranged on the culture carrier. According to the utility model, the adherent culture units are convenient to disassemble and assemble, the liquid supplementing branch pipes are convenient to protect, and part of the liquid supplementing branch pipes can be selectively selected for infusion work, so that the culture scale is convenient to adjust; and more adherent culture units can be arranged in the same space.

Description

Technical Field

[0001] The utility model mainly relates to the technical field of microalgae cultivation equipment, in particular to a bioreactor capable of culturing microalgae on a large scale by adhering to a wall. Background Art

[0002] Microalgae is an aquatic microorganism, mainly single-celled. Traditional large-scale microalgae cultivation adopts an immersion suspension cell culture method. The photobioreactor used must have the ability to accommodate a large volume of culture medium, and its cultivation process requires a large amount of water resources. In order to improve the utilization of light energy of cells and improve the culture efficiency, microalgae adherent culture technology has emerged in the prior art. For example, in the patent with the authorization announcement number CN103289887B, a semi-dry solid adherent culture device for industrial production of microalgae is disclosed, including a support system, an adherent culture unit, a support shaft transmission system, a speed regulating motor, a liquid infusion pipe, a collection tank and a culture medium storage tank, wherein at least one adherent culture unit is installed on the support system, and each adherent culture unit is connected to the speed regulating motor through the support shaft transmission system; a liquid infusion pipe is provided above each adherent culture unit, and each liquid infusion pipe is connected to the culture medium storage tank through a pipeline; a collection tank is provided below each adherent culture unit, and each collection tank is connected to the culture medium storage tank through a liquid collecting pipe; a liquid circulation pump and a carbon replenishing device are respectively provided in the culture medium storage tank. The device can be used for large-scale production of microalgae biomass and secondary metabolites, effectively increasing the yield per unit area, shortening the culture cycle, significantly reducing culture water consumption, and reducing the cost of culture equipment and operating costs.

[0003] However, the above-mentioned wall-adherent culture device has the following problems: (1) The wall-adherent culture unit is located on the inner side of the support system, which is inconvenient to install and disassemble, and thus it is not convenient to adjust the culture scale; (2) The infusion tube is exposed above the wall-adherent culture unit, and the infusion tube cannot be protected; (3) All the infusion tubes perform infusion work simultaneously, and cannot perform infusion work selectively, and thus it is not convenient to adjust the culture scale. Utility Model Content

[0004] The technical solution of the utility model aims at the technical problem that the existing technical solutions are too single, and provides a solution that is significantly different from the existing technology. It mainly provides a bioreactor that can culture microalgae on a large scale, so as to solve the technical problem of the inconvenience of adjusting the culture scale raised in the above background technology.

[0005] The technical solution adopted by the utility model to solve the above technical problems is:

[0006] A bioreactor capable of large-scale adherent culture of microalgae comprises a support system, a wall-adherent culture unit and a culture medium circulation module, wherein the wall-adherent culture unit comprises a culture carrier made of a soft material, and the culture medium circulation module comprises a circulating water tank, a circulating pipeline and a liquid replenishment branch pipe which are connected in sequence; the support system comprises a main support and support branches which are connected to two opposite sides of the main support at equal intervals, each of the support branches is covered with a support body, the culture carrier is mounted on the support body, and a fixing fixture for fitting two halves of the culture carrier together is provided on the culture carrier; the circulating water tank is located directly below all the support branches, the liquid replenishment branches are installed at the bottom of the support branches in a one-to-one correspondence, and the bottom of the liquid replenishment branches are evenly connected with drip irrigation nozzles.

[0007] Furthermore, the support body is a cylindrical structure, the fluid infusion branch is located inside the support body, and a through opening is opened at the bottom of the support body.

[0008] Furthermore, the top of the support body is placed on the bracket branch, the support body is rotatable, and a blocking block for blocking the drip irrigation nozzle is provided on the inner wall of the support body. The inner side surface of the blocking block 13 is an arc surface, and the blocking block 13 is staggered with the opening 12.

[0009] Furthermore, the top of the stent branch 10 is a curved surface 16 coaxially arranged with the support body 9, the curved surface inside the blocking block 13 fits with the curved surface 16, and the central angle corresponding to the curvature of the blocking block 13 is greater than 180°.

[0010] Furthermore, a second limiting block 15 for contacting one side of the bracket branch 10 is fixed to one end of the blocking block 13 , and a first limiting block 14 for contacting the other side of the bracket branch 10 is fixed to the inner side of the blocking block 13 .

[0011] Specifically, the culture carrier is made of a square soft water-permeable material, and the culture carrier is folded in half at the center and hung on the support.

[0012] Specifically, the fixing fixture includes two long strip-shaped clamping plates and locking components arranged at the ends of the clamping plates, and the clamping plates are arranged parallel to the width direction of the culture carrier.

[0013] Specifically, the circulation pipeline includes a liquid supply pipeline connected to the circulation water tank and a liquid infusion main pipe connected to all liquid infusion branches. The liquid infusion main pipe is arranged along the main support, and a circulation pump is provided between the liquid infusion main pipe and the liquid supply pipeline.

[0014] Compared with the prior art, the beneficial effects of the utility model are:

[0015] (1) The support system of the utility model comprises a main support and support branches connected at equal intervals on two opposite sides of the main support, each of the support branches is covered with a support body, and the culture carrier is mounted on the support body. When disassembling and assembling the wall-adherent culture unit, since the support body and the support branches are located on both sides of the main support, the wall-adherent culture unit can be easily put on the support body or removed from the support body. That is, compared with the prior art in which the wall-adherent culture unit is located on the inner side of the support system, the utility model facilitates the disassembly and assembly of the wall-adherent culture unit, thereby facilitating the adjustment of the culture scale.

[0016] (2) The culture carrier of the utility model is provided with a fixing clamp for fitting the two halves of the culture carrier together, and with the function of the support body, the culture carrier above the fixing clamp forms an inverted teardrop shape, and the fluid infusion branch pipe is located on the inner side of the culture carrier above the fixing clamp. Compared with the prior art in which the fluid infusion tube is exposed and located above the wall-attached culture unit, the utility model facilitates the protection of the fluid infusion branch pipe.

[0017] (3) In the present invention, the two halves of the same culture carrier are attached together, which is convenient for simultaneously receiving the culture medium transported by the infusion branch pipe, so that microalgae cells can be inoculated on both halves of the culture carrier, and the microalgae on the two halves of the culture carrier can be uniformly managed. In addition, the two halves of the culture carrier are attached together, which can also significantly increase the distance between two adjacent culture carriers, thereby helping to reduce the space occupied by equipment of the same culture scale, and also allowing more adherent culture units to be arranged in the same space.

[0018] (4) In the optimized solution of the utility model, the support body is set as a cylindrical structure, the liquid infusion branch pipe is located inside the support body, and a through hole is opened at the bottom of the support body to better protect the liquid infusion branch pipe. The setting of the through hole ensures that the culture medium falls onto the culture carrier.

[0019] (5) In a further optimized solution of the utility model, a support body is arranged to be rotatable relative to the support branch, and a blocking block for blocking the drip irrigation nozzle is arranged on the inner wall of the support body, and the blocking block is staggered with the opening. When some of the support bodies and the support branches are selected to participate in the work, for the selected support body, the support body is rotated until the opening is vertically aligned with the drip irrigation nozzle, so that the liquid culture medium falls on the culture carrier; for the vacant support body, the support body is rotated until the blocking block fits with the drip irrigation nozzle to achieve the blocking of the drip irrigation nozzle, and the liquid culture medium is not allowed to drip from this area (that is, the infusion work of the corresponding infusion branch is prevented). It can be seen from this that the present device can selectively select some of the infusion branches for infusion work, thereby facilitating the adjustment of the culture scale. In addition, the selection of the corresponding infusion branch can be adjusted by rotating the support body to different positions, which is simple to operate and easy to use.

[0020] The present invention will be explained in detail below with reference to the accompanying drawings and specific embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a schematic diagram of the three-dimensional structure of the utility model in the second embodiment;

[0022] Figure 2 It is a three-dimensional structural schematic diagram of the arrangement of the liquid replenishment pipe (including the liquid replenishment main pipe and the liquid replenishment branch pipe) and the drip irrigation nozzle according to the bracket system in the utility model;

[0023] Figure 3 This is the front view of the second embodiment of the present utility model;

[0024] Figure 4 It is a schematic diagram of the support body and its internal components in the second embodiment of the present utility model;

[0025] Figure 5 This is a schematic diagram of the support body and its internal components in the third embodiment of the present invention, at which time the liquid infusion branch pipe and the drip irrigation nozzle are in a normal liquid infusion working state;

[0026] Figure 6 This is a schematic diagram of the support body and its internal components in the third embodiment of the present invention, at which time the liquid replenishment branch pipe and the drip irrigation nozzle are in a non-working state.

[0027] Reference numerals:

[0028] 1. Main support; 2. Fluid infusion branch pipe; 3. Fixing fixture; 4. Culture carrier; 5. Circulating water tank; 6. Fluid supply pipeline; 7. Circulating pump; 8. Fluid infusion main pipe; 9. Support body; 10. Support branch; 11. Drip irrigation nozzle; 12. Through port; 13. Blocking block; 14. First limit block; 15. Second limit block; 16. Arc surface. DETAILED DESCRIPTION

[0029] In order to facilitate the understanding of the present invention, the present invention will be described in more detail below with reference to the relevant drawings. Several embodiments of the present invention are given in the drawings, but the present invention can be implemented in different forms and is not limited to the embodiments described in the text. On the contrary, these embodiments are provided to make the content disclosed in the present invention more thorough and comprehensive.

[0030] It should be noted that when an element is referred to as being "fixed on" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used in this article are for illustrative purposes only.

[0031] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly connected by technicians in the technical field of the present invention. The terminology used in the specification of the present invention is for the purpose of describing specific embodiments and is not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0032] Embodiment 1: A bioreactor capable of large-scale adherent culture of microalgae, comprising a support system, an adherent culture unit and a culture medium circulation module; specifically:

[0033] Please refer to the attached Figure 1 -Attached Figure 3 The support system provides a mounting function for the wall-attached culture unit. The support system is a "fishbone-shaped" structure, including a main support 1 in a door frame structure and a plurality of support branches 10 equidistantly connected to two opposite sides of the transverse part of the main support 1. Both ends of the main support 1 are supported on the ground, and each of the support branches 10 is covered with a support body 9. The width of the support body 9 is greater than that of the support branch 10, and the upper part of the support body 9 is an arc-shaped arch surface, which is used to mount the culture carrier 4 and directly contact the culture carrier 4. At the same time, it can support a larger space at the upper end of the culture carrier 4, which is convenient for the setting of the infusion branch 2 and is also beneficial for protecting the infusion branch 2; the material of the support system is assembled from hard salt-resistant and corrosion-resistant materials, including but not limited to stainless steel, plastic, polymer materials, etc.

[0034] Please refer to the attached Figure 1 and attached Figure 3The wall-attached culture unit includes a culture carrier 4 made of a soft and permeable material, which can provide a wall-attached attachment position for microalgae cells, and the cells can adhere to the carrier material to achieve wall-attached reproduction. The culture carrier 4 is a square soft and permeable material, which is folded in half at the center and mounted on the support body 9. The culture carrier 4 has the following characteristics: soft, high friction, permeable, foldable, low ductility, not easy to break, salt-resistant and corrosion-resistant. The material of the culture carrier 4 includes but is not limited to: cloth, non-woven fabric, glass fiber, polymer material, etc. The culture carrier 4 is provided with a fixing fixture 3 for fitting the two halves of the culture carrier 4 together, and the culture carrier 4 above the fixing fixture 3 forms an inverted water drop shape. The fixing fixture 3 is used to fix the position of the culture carrier 4 to ensure that the culture carrier 4 will not be offset during use; and the two halves of the culture carrier 4 are fitted together, which is convenient for simultaneously receiving the culture medium transported by the infusion branch 2, so that microalgae cells can be inoculated on both halves of the culture carrier 4, and it is convenient to uniformly manage the microalgae on the two halves of the culture carrier 4. The fixing fixture 3 is made of hard material, is in the shape of a strip and has a clamping function in pairs, and is also salt-resistant and corrosion-resistant. Its materials include but are not limited to: plastic, stainless steel, acrylic, polymer materials, etc. The fixing fixture 3 includes two strip-shaped clamps and locking components (such as press-type buckles) arranged at the ends of the clamps. The clamps are arranged parallel to the width direction of the culture carrier 4.

[0035] Please refer to the attached Figure 1 -Attached Figure 3 The culture medium circulation module is a circulation system for continuously supplying liquid culture medium to the microalgae cells on the culture carrier 4, including a circulating water tank 5, a circulating pipe and a liquid replenishment branch pipe 2 connected in sequence, the circulating pipe includes a liquid supply pipe 6 connected to the circulating water tank 5 and a liquid replenishment main pipe 8 connected to all the liquid replenishment branches 2, and a circulation pump 7 is provided between the liquid replenishment main pipe 8 and the liquid supply pipe 6. The circulating water tank 5 is located on the ground directly below all the support branches 10, and is used to receive the culture solution dripping from the culture carrier 4; the liquid replenishment main pipe 8 is arranged along the main support 1, and the liquid replenishment branch pipes 2 are installed one-to-one at the bottom of the support branch 10. The bottom of the liquid replenishment branch pipe 2 is evenly connected with a drip irrigation nozzle 11, and the drip irrigation nozzle 11 is vertically downward. When the circulation pump 7 is turned on, the culture medium can be drip-irrigated to the upper part of the culture carrier 4. The culture medium flows to the lower part of the culture carrier 4 under the action of gravity and then falls into the circulating water tank 5, and then the culture medium is transported to the upper part of the culture carrier 4 by the circulation pump 7. During the circulation process, the culture medium continuously provides water and nutrients to the microalgae cells on the culture carrier 4.

[0036] When the above-mentioned bioreactor is used, the culture carriers 4 can be mounted on all the supports 9 in a one-to-one correspondence, or the culture carriers 4 can be mounted on some of the supports 9 as needed, and the breeding scale can be adjusted by controlling the number of culture carriers 4. At the same time, the gap between the culture carriers 4 can be adjusted by controlling the number of support bodies 9 between each two adjacent culture carriers 4, so as to provide suitable spacing space for microalgae cells with different needs. When disassembling the wall-attached culture unit, since the support body 9 and the support branch 10 are located on both sides of the main support 1, the culture carrier 4 (with the fixing clamp 3 pre-installed) can be easily put on the support body 9 or the culture carrier 4 (with the fixing clamp 3) can be removed from the support body 9, which is convenient for disassembling the wall-attached culture unit, thereby facilitating the adjustment of the culture scale.

[0037] Embodiment 2: This embodiment differs from Embodiment 1 in that:

[0038] In this embodiment, please refer to the attached Figure 4 The support body 9 is a cylindrical structure, and the liquid infusion branch 2 is located inside the support body 9. The support body 9 can achieve a more comprehensive protection effect on the liquid infusion branch 2 while meeting the mounting requirements of the culture carrier 4. A through hole 12 is provided at the bottom of the support body 9, which is vertically aligned with the drip irrigation nozzle 11, so that the liquid culture medium falls on the culture carrier 4.

[0039] In this embodiment, please refer to the attached Figure 1 A circulating water tank 5 is provided on both sides of the main support 1 , and the two circulating water tanks 5 are connected through a pipeline, and one of the circulating water tanks 5 is connected to the liquid supply pipeline 6 .

[0040] The rest is the same as the first embodiment.

[0041] Embodiment 3: This embodiment differs from Embodiment 2 in that:

[0042] In this embodiment, please refer to the attached Figure 5 and attached Figure 6The top of the support body 9 is placed on the bracket branch 10, and the support body 9 is rotatable. A blocking block 13 for blocking the drip irrigation nozzle 11 is provided on the inner wall of the support body 9. The inner side surface of the blocking block 13 is an arc surface, and the blocking block 13 is staggered with the through-port 12. For the support body 9 with the culture carrier 4 mounted thereon, the support body 9 is rotated until the through-port 12 is located at its bottom, that is, vertically aligned with the drip irrigation nozzle 11, so that the liquid culture medium falls on the culture carrier 4; for the support body 9 without the culture carrier 4 mounted thereon, the support body 9 is rotated until the blocking block 13 is located at its bottom, that is, the blocking block 13 is fitted with the drip irrigation nozzle 11 to achieve the blocking of the drip irrigation nozzle 11, and the liquid culture medium is not allowed to drip from this area (that is, the infusion work of the corresponding infusion branch 2 is prevented). It can be seen from this that the device can selectively select part of the infusion branch 2 for infusion work, thereby facilitating the adjustment of the culture scale. In addition, the selection of the corresponding infusion branch pipe 2 can be adjusted by rotating the support body 9 to different positions, which is easy to operate and convenient to use.

[0043] In this embodiment, the top of the support branch 10 is a curved surface 16 coaxially arranged with the support body 9, the curved surface inside the blocking block 13 fits with the curved surface 16, and the central angle corresponding to the curvature of the blocking block 13 is greater than 180°. This arrangement is convenient for improving the stability of the support body 9 rotating on the support branch 10, and the central angle of the blocking block 13 is limited to ensure that after the blocking block 13 blocks the drip irrigation nozzle 11, part of the blocking block 13 can still fit the curved surface 16 and effectively hang on the support branch 10.

[0044] In this embodiment, a second stopper 15 for contacting one side of the support branch 10 is fixed to one end of the blocking block 13, and a first stopper 14 for contacting the other side of the support branch 10 is fixed to the inner side of the blocking block 13. When the support body 9 rotates to the point where the first stopper 14 contacts one side of the support branch 10, the through opening 12 is just facing the drip irrigation nozzle 11; when the second stopper 15 contacts one side of the support branch 10, the blocking block 13 rotates to block the drip irrigation nozzle 11, that is, the final adjustment position of the rotating support body 9 can be determined by the first stopper 14 and the second stopper 15, which is convenient for operation.

[0045] The rest is the same as the second embodiment.

[0046] The above description of the utility model in combination with the accompanying drawings is an exemplary description. Obviously, the specific implementation of the utility model is not limited to the above-mentioned method. As long as the non-substantial improvements are made by adopting the method concept and technical solution of the utility model, or the concept and technical solution of the utility model are directly applied to other occasions without improvement, they are all within the protection scope of the utility model.

Claims

1. A bioreactor capable of culturing microalgae on a large scale by adhering to a wall, comprising a support system, an adherent culture unit and a culture medium circulation module, wherein the adherent culture unit comprises a culture carrier (4) made of a soft material, and the culture medium circulation module comprises a circulating water tank (5), a circulating pipeline and a liquid replenishment branch pipe (2) which are connected in sequence; characterized in that: The support system comprises a main support (1) and support branches (10) connected at equal intervals on two opposite sides of the main support (1); each of the support branches (10) is covered with a support body (9); the culture carrier (4) is mounted on the support body (9); and the culture carrier (4) is provided with a fixing fixture (3) for fitting two halves of the culture carrier (4) together; the circulating water tank (5) is located directly below all the support branches (10); the liquid replenishment branch pipes (2) are installed in a one-to-one correspondence at the bottom of the support branches (10); and the bottom of the liquid replenishment branch pipes (2) are evenly connected with drip irrigation nozzles (11).

2. A bioreactor capable of culturing microalgae on a large scale according to claim 1, characterized in that: The support body (9) is a cylindrical structure, the fluid infusion branch pipe (2) is located inside the support body (9), and a through opening (12) is provided at the bottom of the support body (9).

3. A bioreactor capable of culturing microalgae on a large scale according to claim 2, characterized in that: The top of the support body (9) is placed on the bracket branch (10), and the support body (9) is rotatable. A blocking block (13) for blocking the drip irrigation nozzle (11) is provided on the inner wall of the support body (9), and the inner side surface of the blocking block (13) is an arc-shaped surface. The blocking block (13) and the opening (12) are arranged in a staggered manner.

4. The bioreactor capable of culturing microalgae on a large scale according to claim 3, characterized in that: The top of the stent branch (10) is a curved surface (16) coaxially arranged with the support body (9), the curved surface inside the blocking block (13) fits the curved surface (16), and the central angle corresponding to the arc of the blocking block (13) is greater than 180°.

5. The bioreactor capable of culturing microalgae on a large scale according to claim 4, characterized in that: A second limit block (15) for contacting one side of the support branch (10) is fixed to one end of the blocking block (13), and a first limit block (14) for contacting the other side of the support branch (10) is fixed to the inner side of the blocking block (13).

6. A bioreactor capable of culturing microalgae on a large scale according to any one of claims 1 to 5, characterized in that: The culture carrier (4) is made of a square soft water-permeable material, and the culture carrier (4) is folded in half at the center and hung on the support body (9).

7. A bioreactor capable of culturing microalgae on a large scale according to any one of claims 1 to 5, characterized in that: The fixing clamp (3) comprises two long strip-shaped clamping plates and locking components arranged at the ends of the clamping plates, and the clamping plates are arranged parallel to the width direction of the culture carrier (4).

8. A bioreactor capable of culturing microalgae on a large scale according to any one of claims 1 to 5, characterized in that: The circulation pipeline comprises a liquid supply pipeline (6) connected to the circulation water tank (5) and a liquid infusion main pipe (8) connected to all the liquid infusion branch pipes (2); the liquid infusion main pipe (8) is arranged along the main support (1); and a circulation pump (7) is provided between the liquid infusion main pipe (8) and the liquid supply pipeline (6).

Images