Plant protease separation and purification device

Abstract

The utility model discloses a plant protease separation and purification device which comprises a separation box, a liquid outlet is formed in the bottom of the separation box, a motor is arranged at the bottom of the separation box, a rotating shaft is arranged at the output end of the motor, a separation net cylinder is arranged at the upper end of the rotating shaft, and a support is arranged on the side wall of the separation box. An electric telescopic rod is arranged on the support, a scraping and brushing device is arranged at the lower end of the electric telescopic rod and located in the separation net cylinder, and limiting devices are arranged on the side wall of the separation box and the outer wall of the separation net cylinder. The utility model belongs to the technical field of protease preparation, and particularly relates to a plant protease separation and purification device which solves the problems that in the prior art, when a protease solution in plants is filtered, the filtering speed is low, filter residues are prone to blocking a filter screen, and the filter residues are inconvenient to clean out.

Description

Technical Field

[0001] The utility model belongs to the technical field of protease preparation, and specifically refers to a plant protease separation and purification device. Background Art

[0002] The role of proteases in plant roots is actually very important. First, proteases can break down proteins in the root system, thereby providing the plants with the nutrients they need. Secondly, proteases can also promote the formation and renewal of plant cell walls, thereby improving the plant's disease resistance and ability to adapt to the environment. Finally, proteases' regulation of the root system also helps to increase plant growth and optimize plant yields. Proteases are widely present in plant stems, leaves and fruits.

[0003] In the process of extracting plant proteases, the plant tissues must first be crushed, and then a clarifying liquid agent is added, and the liquid is introduced into the enzyme extraction equipment to finally obtain a high-protease liquid. After separation and purification, the protease is finally obtained. In the prior art, the separation and purification of proteases mostly use ultrafine filters or filter paper for separation and purification, but there are some shortcomings: since the protease solution contains plant debris, it needs to be filtered first. During the filtration process, the filter is relatively fine, resulting in a very slow filtration speed, and the filter residue is also easy to clog the filter. At the same time, the filter residue is not easy to clean out, so improvements are needed. Utility Model Content

[0004] The technical problem to be solved by the utility model is that in the prior art, when filtering the protease solution in plants, the filtering speed is slow, the filter residue is easy to block the filter screen, and the filter residue is not easy to clean out.

[0005] In order to achieve the above functions, the technical solution adopted by the utility model is as follows: a plant protease separation and purification device, comprising a separation box, a drain port is provided at the bottom of the separation box, a motor is provided at the bottom of the separation box, a rotating shaft is provided at the output end of the motor, a separation net cylinder is provided at the upper end of the rotating shaft, a bracket is provided on the side wall of the separation box, an electric telescopic rod is provided on the bracket, a scraping device is provided at the lower end of the electric telescopic rod and is located inside the separation net cylinder, and a limiting device is provided on the side wall of the separation box and the outer wall of the separation net cylinder; the scraping device comprises a connecting rod, the connecting rod runs through the bracket, a piston plate is provided at the lower end of the connecting rod, a sleeve is provided on the side wall of the connecting rod, a telescopic column is slidably provided in the sleeve, a connecting rod is provided at one end of the telescopic column, a scraping brush is provided on the connecting rod, a spring is provided at the other end of the telescopic column, and the other end of the spring is fixed in the sleeve, the inner wall of the separation net cylinder can be cleaned by the scraping brush to keep the separation net cylinder from being blocked, and the separation net cylinder is driven to rotate by the motor to accelerate the filtration and separation of the protease solution in the separation net cylinder.

[0006] Furthermore, the limiting device includes a limiting seat, a limiting groove is provided in the limiting seat, a limiting plate is provided on the outer wall of the upper end of the separation net cylinder, and the limiting plate rotates in the limiting groove. The limiting seat can ensure the stability of the separation net cylinder during high-speed operation.

[0007] Preferably, a cover plate is provided at the upper end of the connecting rod, and when the piston plate moves down to the bottom of the separation net cylinder, the cover plate can seal the separation net cylinder to prevent the protease solution from splashing upwards during centrifugal separation.

[0008] Preferably, the telescopic column is a rectangular columnar rod, and an end plate is provided at the end of the telescopic column. The telescopic column cannot rotate in the sleeve, and the end plate can prevent the telescopic column from detaching from the sleeve.

[0009] Preferably, the spring always exerts elastic force on the telescopic column to ensure that the scraping brush is always in close contact with the inner wall of the separation net cylinder.

[0010] Preferably, the limit seats are provided in a plurality of groups in a circumferential array on the inner wall of the separation box.

[0011] Preferably, an observation window is provided on the outer wall of the separation box.

[0012] The utility model adopts the above structure to achieve the following beneficial effects:

[0013] 1. The utility model can scrape and brush the separation net cylinder in a rotating state by arranging a scraping and brushing device to ensure that the separation net cylinder will not be blocked by the filter residue in the protease solution. By pulling the piston plate upward with the help of an electric telescopic rod, the piston plate can pull the filter residue out of the separation net cylinder for cleaning.

[0014] 2. The utility model ensures the stability of the separation net cylinder when it runs at high speed by setting a limit device. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is an overall stereogram of a plant protease separation and purification device of the utility model;

[0016] Figure 2 for Figure 1 Schematic diagram of the internal structure;

[0017] Figure 3 for Figure 2 A perspective view of the middle scraper and brush device;

[0018] Figure 4 for Figure 2 Three-dimensional cross-sectional view of the middle limit seat.

[0019] Among them, 1. separation box, 2. drain port, 3. motor, 4. rotating shaft, 5. separation net cylinder, 6. bracket, 7. electric telescopic rod, 8. limit device, 9. scraper device, 10. connecting rod, 11. piston plate, 12. sleeve, 13. telescopic column, 14. connecting rod, 15. scraper, 16. spring, 17. limit seat, 18. limit groove, 19. limit plate, 20. cover plate, 21. end plate, 22. observation window. DETAILED DESCRIPTION

[0020] The technical solution of the utility model will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0021] In the description of the present invention, it should be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside", etc. indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they cannot be understood as limitations on the present invention.

[0022] like Figure 1-4 The utility model discloses a plant protease separation and purification device, comprising a separation box 1, a liquid discharge port 2 is provided at the bottom of the separation box 1, an observation window 22 is provided on the outer wall of the separation box 1, a motor 3 is provided at the bottom of the separation box 1, a rotating shaft 4 is provided at the output end of the motor 3, a separation net cylinder 5 is provided at the upper end of the rotating shaft 4, a bracket 6 is provided on the side wall of the separation box 1, an electric telescopic rod 7 is provided on the bracket 6, a scraping device 9 is provided at the lower end of the electric telescopic rod 7 and is located inside the separation net cylinder 5, and a limiting device 8 is provided on the side wall of the separation box 1 and on the outer wall of the separation net cylinder 5;

[0023] like Figure 1-3The scraping device 9 includes a connecting rod 10, which runs through the bracket 6. A piston plate 11 is provided at the lower end of the connecting rod 10, and a cover plate 20 is provided at the upper end of the connecting rod 10. When the piston plate 11 moves down to the bottom of the separation net cylinder 5, the cover plate 20 can cover the separation net cylinder 5 to prevent the protease solution from splashing upward during centrifugal separation. A sleeve 12 is provided on the side wall of the connecting rod 10, and a telescopic column 13 is slidably provided in the sleeve 12. A connecting rod 14 is provided at one end of the telescopic column 13. The telescopic column 13 is a rectangular columnar rod. An end plate 21 is provided at the end of the telescopic column 13. The telescopic column 13 cannot be in the sleeve. The end plate 21 can prevent the telescopic column 13 from being separated from the sleeve 12 when the telescopic column 13 rotates in the sleeve 12. A scraper 15 is provided on the connecting rod 14. A spring 16 is provided at the other end of the telescopic column 13. The other end of the spring 16 is fixedly connected to the sleeve 12. The spring 16 always has elastic force on the telescopic column 13 to ensure that the scraper 15 is always in close contact with the inner wall of the separation net cylinder 5. The scraper 15 can be used to clean the inner wall of the separation net cylinder 5 to keep the separation net cylinder 5 from being blocked. The separation net cylinder 5 is driven to rotate by the motor 3 to speed up the filtration and separation of the protease solution in the separation net cylinder 5.

[0024] like Figure 1 , 2 And 4, the limiting device 8 includes a limiting seat 17, and a plurality of limiting seats 17 are arranged in a circular array on the inner wall of the separation box 1, and a limiting groove 18 is arranged in the limiting seat 17. A limiting plate 19 is arranged on the outer wall of the upper end of the separation net cylinder 5, and the limiting plate 19 rotates in the limiting groove 18. The limiting seat 17 can ensure the stability of the separation net cylinder 5 when it runs at high speed.

[0025] During specific use, the protease solution to be separated and purified is poured into the separation net cylinder 5 for filtration, the motor 3 is started, and the rotating shaft 4 drives the separation net cylinder 5 to rotate at high speed. The limiting plate 19 will always rotate in the limiting groove 18, and the limiting seat 17 can ensure that the separation net cylinder 5 will not shake during rotation, thereby ensuring stability.

[0026] When the separation net cylinder 5 rotates at high speed, due to the action of centrifugal force, the protease solution will be quickly thrown out, accelerating the filtration and separation of the protease solution. At the same time, due to the action of the spring 16, the scraper 15 on the connecting rod 14 will always be in close contact with the inner wall of the separation net cylinder 5 for scraping 15, which can clean the filter residue in the separation net cylinder 5 to avoid clogging.

[0027] When the filter residue in the separation net cylinder 5 needs to be cleaned, the electric telescopic rod 7 pulls the connecting rod 10, and the connecting rod 10 pulls the piston plate 11 to move upward from the separation net cylinder 5, and the piston plate 11 pulls the filter residue out of the separation net cylinder 5 for centralized cleaning. The protease solution in the separation box 1 can be discharged through the drain port 2.

[0028] The above description of the utility model and its implementation methods is not restrictive. The drawings show only one implementation method of the utility model, and the actual structure is not limited thereto. In short, if ordinary technicians in this field are inspired by it and design structural methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention of the utility model, they should all fall within the protection scope of the utility model.

Claims

1. A plant protease separation and purification device, comprising a separation box, wherein a drain port is provided at the bottom of the separation box, characterized in that: A motor is provided at the bottom of the separation box, a rotating shaft is provided at the output end of the motor, a separation net cylinder is provided at the upper end of the rotating shaft, a bracket is provided on the side wall of the separation box, an electric telescopic rod is provided on the bracket, a scraping brush device is provided at the lower end of the electric telescopic rod and is located inside the separation net cylinder, and a limiting device is provided on the side wall of the separation box and on the outer wall of the separation net cylinder.

2. A plant protease separation and purification device according to claim 1, characterized in that: The scraping device includes a connecting rod, which runs through the bracket. A piston plate is provided at the lower end of the connecting rod. A sleeve is provided on the side wall of the connecting rod. A telescopic column is slidably provided in the sleeve. A connecting rod is provided at one end of the telescopic column. A scraping brush is provided on the connecting rod. A spring is provided at the other end of the telescopic column. The other end of the spring is fixedly connected to the sleeve.

3. A plant protease separation and purification device according to claim 2, characterized in that: The limiting device comprises a limiting seat, a limiting groove is arranged in the limiting seat, a limiting plate is arranged on the outer wall of the upper end of the separation net cylinder, and the limiting plate rotates in the limiting groove.

4. A plant protease separation and purification device according to claim 3, characterized in that: A cover plate is provided at the upper end of the rod, and when the piston plate moves down to the bottom of the separation net cylinder, the cover plate can seal the separation net cylinder.

5. A plant protease separation and purification device according to claim 4, characterized in that: The telescopic column is a rectangular columnar rod, and an end plate is provided at the end of the telescopic column.

6. A plant protease separation and purification device according to claim 5, characterized in that: The spring always exerts elastic force on the telescopic column.

7. A plant protease separation and purification device according to claim 6, characterized in that: The limit seats are arranged in a plurality of groups in a circumferential array on the inner wall of the separation box.

8. A plant protease separation and purification device according to claim 7, characterized in that: An observation window is arranged on the outer wall of the separation box.

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