Cell culture device
By arranging a moisturizing insert box and a magnetic levitation shock absorber in the cell culture device, the problem of difficulty in maintaining the internal humidity and stability of the cell culture device in the existing technology is solved, and a better cell culture environment is achieved.
Patent Information
- Application Number
- CN202421087021.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-20
- Publication Date
- 2025-01-21
- Estimated Expiration
- 2034-05-20
AI Technical Summary
Existing cell culture devices are difficult to maintain internal humidity at 100%, resulting in unstable cell culture environment and affecting cell growth.
A cell culture device is designed, which includes a box body and a lid body that interlock with each other. Inside the box body, there is a wall compartment and a moisturizing box. The moisturizing box is equipped with moisturizing gel beads, and a magnetic levitation box is installed at the bottom of the box. Shock absorber to filter out small vibrations.
The moisturizing gel beads in the moisturizing insert box provide sufficient water vapor to ensure that the inside of the box maintains saturated humidity, reduce evaporation, and protect the cell culture environment; the magnetic levitation shock absorber filters small vibrations and provides a stable cell growth environment.
Smart Images
Figure CN222374667U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of cell biology and relates to a cell culture device. Background Art
[0002] Cell culture refers to a method of simulating the in vivo environment in an in vitro environment to allow cells to survive, grow, reproduce and maintain their main structures and functions. It is an essential experimental process in biomedical research.
[0003] The culture of mammalian cells generally needs to be kept in a dark environment at 37°C and 100% humidity. In actual experiments, in order to ensure the humidity of cell culture, a water tray is usually placed at the bottom of the cell culture incubator, and the natural evaporation of the water tray is used to keep the inside of the cell culture incubator at a certain humidity. However, under normal circumstances, the humidity inside the cell culture incubator does not reach 100%, especially the cell bottles or culture dishes on the upper layer of the cell culture incubator have excessive evaporation due to insufficient humidity. Excessive evaporation of water inside the cell culture incubator will change the osmotic pressure of the culture medium and affect cell culture. Therefore, for cells, especially precious cells, it is necessary to further maintain local 100% humidity to maintain the osmotic pressure of the culture system.
[0004] In addition, conventional laboratories also place cell dishes, cell bottles or culture plates in aluminum lunch boxes and place wet gauze on the bottom of the aluminum lunch box to maintain humidity. However, this method is prone to contamination and the ratio of air in the box to the air in the incubator is inconsistent. At the same time, the box is affected by the slight vibration of the test bench, which will also affect the physiological activities of cells to a certain extent. Utility Model Content
[0005] The utility model aims to provide a cell culture device to solve the problem that the existing cell culture device is difficult to maintain the humidity inside the device at 100%.
[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:
[0007] The utility model provides a cell culture device, comprising: a box body and a cover body which are interlocked with each other, wherein two opposite side walls inside the box body are provided with plug-in box wall bins, a moisturizing plug-in box is placed inside the plug-in box, and moisturizing gel beads are arranged inside the moisturizing plug-in box; a bracket is also arranged inside the box body, and the bracket is located between the two plug-in box wall bins.
[0008] Preferably, a plurality of through holes are provided on the side wall of the moisturizing plug-in box.
[0009] Preferably, a handle is provided on the top of the moisturizing plug-in box.
[0010] Preferably, the moisturizing gel beads are polyacrylamide gel beads or polyacrylate gel beads.
[0011] Preferably, a tenon is provided inside the cover body, and the box body and the cover body that are snapped together are connected to the outside of the box body through the tenon.
[0012] Preferably, the box body and the cover body are both dark-colored box body and dark-colored cover body.
[0013] Preferably, shock absorbing chambers are respectively arranged at the four corners of the bottom of the box body, and magnetic suspension shock absorbers are arranged in the shock absorbing chambers. The magnetic suspension shock absorbers are exposed at the bottom of the box body.
[0014] Preferably, the magnetic suspension shock absorber includes a magnetic suspension shock absorber cylinder and a first magnet and a second magnet located on the top of the magnetic suspension shock absorber cylinder; the magnetic suspension shock absorber cylinder is exposed at the bottom of the box body, and the magnetic poles of the first magnet and the second magnet are arranged opposite to each other.
[0015] The utility model has the following beneficial effects:
[0016] (1) The moisturizing gel beads set in the moisturizing plug box can provide sufficient water vapor to the inside of the box, ensuring that the inside of the box maintains saturated humidity, reducing evaporation, and is more conducive to maintaining the osmotic pressure of the culture medium.
[0017] (2) The setting of the magnetic levitation shock absorber can filter out tiny vibrations and prevent them from being transmitted to the box body, thereby ensuring a stable environment for cell growth.
[0018] (3) The setting of the latch can not only maintain air circulation inside and outside the box, but also prevent microorganisms from entering the cell culture device and contaminating the cultured cells. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] Figure 1 An exploded view of the structure of the cell culture device provided in an embodiment of the present application;
[0020] Figure 2 A schematic diagram of the structure inside the box provided in an embodiment of the present application;
[0021] Figure 3 A schematic diagram of the three-dimensional structure of the moisturizing plug-in box provided in an embodiment of the present application;
[0022] Figure 4 A cross-sectional view of a moisturizing plug-in box provided in an embodiment of the present application;
[0023] Figure 5 A schematic diagram of the structure of the cover body provided in an embodiment of the present application;
[0024] Figure 6 A schematic diagram of the structure of a magnetic suspension shock absorber provided in an embodiment of the present application;
[0025] Symbols represent:
[0026] 1-box body, 2-cover body, 3-insertion box wall compartment, 4-moisturizing insertion box, 5-moisturizing gel beads, 6-bracket, 7-through hole, 8-handle, 9-tenon, 10-shock absorption compartment, 11-magnetic suspension shock absorber, 12-cell culture dish;
[0027] 1101-magnetic levitation shock absorber, 1102-first magnet, 1103-second magnet. DETAILED DESCRIPTION
[0028] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0029] Please refer to the attached Figure 1 , attached Figure 1 The structure explosion diagram of the cell culture device provided in the embodiment of the present application is shown. Figure 1 It can be seen that the cell culture device provided in the embodiment of the present application includes a box body 1 and a cover body 2 that are interlocked. The shape of the box body 1 can be set to be rectangular or square, and the shape of the cover body 2 is set accordingly according to the shape of the box body 1. In order to reduce the influence of light on the cells cultured in the cell culture device, the box body 1 and the cover body 2 are both dark box bodies and dark covers.
[0030] The two opposite walls of the box body 1 are provided with a plug-in box wall compartment 3, in which a moisturizing plug-in box 4 is placed, and a moisturizing gel bead 5 is provided in the moisturizing plug-in box 4. Figure 2 In the embodiment of the present application, the moisturizing gel beads 5 are gel beads made of hydrophilic water-absorbing moisturizing resin such as polyacrylamide gel beads or polyacrylate gel beads, and are used to provide sufficient water vapor to the inside of the box body 1, thereby ensuring that the inside of the box body 1 maintains saturated humidity.
[0031] Further, in order to ensure that the moisturizing gel beads 5 can discharge the evaporated water vapor to the outside of the moisturizing plug box 4, a plurality of through holes 7 are provided on the side wall of the moisturizing plug box 4. Figure 3 , 4 At the same time, the provision of the through holes 7 can also achieve partial fixation of the moisturizing gel beads 5 .
[0032] Furthermore, for the convenience of replacing the moisturizing plug box 4, a handle 8 is provided on the top of the moisturizing plug box 4. By pulling the handle 8, the moisturizing plug box 4 can be extracted from the plug box wall bin 3, so as to replenish water inside the box body 1.
[0033] A bracket 6 is also provided inside the box body 1, and the bracket 6 is located between the two insert box wall compartments 3. In the embodiment of the present application, the bracket 6 is used to place cell culture bottles, cell culture dishes or culture plates, etc., so that the cell culture dishes 12, etc. are located between the two insert box wall compartments 3, and then the moisturizing gel beads 5 ensure that the cells inside the cell culture dishes 12, etc. are in a sufficient humidity environment. In addition, the bracket 6 can also be provided with a handle to facilitate the removal or observation of the growth of cells inside the cell culture dishes 12, etc.
[0034] When the cover 2 is buckled on the box body 1, in order to maintain air circulation inside and outside the box body 1, a latch 9 is provided inside the cover 2. Figure 5 As shown. Thus, the box body 1 and the cover body 2 that are interlocked are connected to the outside of the box body 1 through the tenon 9. At the same time, the arrangement of the tenon 9 can also prevent microorganisms from entering the interior of the cell culture device, thereby preventing the cultured cells from being contaminated.
[0035] In addition, in the embodiment of the present application, a shock absorbing chamber 10 is respectively provided at the four corners of the bottom of the box body 1, and a magnetic levitation shock absorber 11 is provided in the shock absorbing chamber 10, and the magnetic levitation shock absorber 11 is exposed at the bottom of the box body 1. When the cell culture device provided in the embodiment of the present application is used to culture cells, the cell culture device is placed on the test bench through the magnetic levitation shock absorber 11. When the test bench generates a small vibration, the generated small vibration will first be transmitted to the magnetic levitation shock absorber 11, and then the magnetic levitation shock absorber 11 will filter out the vibration and then transmit it to the box body 1, so as to minimize the adverse effects of vibration on the cells cultured in the cell culture device.
[0036] Specifically, the magnetic suspension shock absorber 11 in the embodiment of the present application includes a magnetic suspension shock absorber cylinder 1101 and a first magnet 1102 and a second magnet 1103 located at the top of the magnetic suspension shock absorber cylinder 1101, as shown in the attached Figure 6 As shown, that is, Figure 2 sectional view. The magnetic suspension shock absorber cylinder 1101 is exposed at the bottom of the box body 1, and serves as a supporting leg to support the box body 1. The magnetic poles of the first magnet 1102 and the second magnet 1103 are arranged opposite to each other. Thus, when the magnetic suspension shock absorber cylinder 1101 is vibrated, the repulsive force of the first magnet 1102 and the second magnet 1103, which are arranged opposite to each other, buffers the vibration of the box body 1 by the magnetic suspension shock absorber cylinder 1101, thereby minimizing the vibration of the box body 1. More specifically, the first magnet 1102 is arranged at the top of the shock absorber chamber 10, and the second magnet 1103 is arranged at the top of the magnetic suspension shock absorber cylinder 1101.
[0037] When the cell culture device provided in the embodiment of the present application is in use, the box body 1 is placed on the test bench through the magnetic levitation shock absorber 11, and the moisturizing plug-in box 4 containing moisturizing gel beads 5 is inserted into the plug-in box wall compartment to maintain the saturated humidity inside the box body 1. Then the cell culture dish 12 and the like are placed on the bracket 6, and the bracket 6 is placed between the two moisturizing plug-ins 4 inside the box body 1. After covering the cover body 2, the cell culture device is placed in a cell culture incubator for culture to maintain a constant temperature inside the box body 1. The cell culture device provided in the embodiment of the present application has a simple structure and can stably maintain the saturated humidity inside the cell culture device; at the same time, the setting of the magnetic levitation shock absorber 11 can avoid the influence of small vibrations on cell culture.
[0038] The above description is only the preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims
1. A cell culture device, characterized in that: include: A box body (1) and a cover body (2) are interlocked, and two opposite side walls inside the box body (1) are provided with plug-in box wall bins (3), and a moisturizing plug-in box (4) is placed inside the plug-in box wall bin (3), and moisturizing gel beads (5) are arranged inside the moisturizing plug-in box (4); a bracket (6) is also arranged inside the box body (1), and the bracket (6) is located between the two plug-in box wall bins (3).
2. The cell culture device according to claim 1, characterized in that: A plurality of through holes (7) are provided on the side wall of the moisturizing plug box (4).
3. The cell culture device according to claim 1, characterized in that: A handle (8) is provided on the top of the moisturizing plug box (4).
4. The cell culture device according to claim 1, characterized in that: The moisturizing gel beads (5) are polyacrylamide gel beads or polyacrylate gel beads.
5. The cell culture device according to claim 1, characterized in that: A latch (9) is provided inside the cover body (2), and the box body (1) and the cover body (2) are connected to each other and are connected to the outside of the box body (1) via the latch (9).
6. The cell culture device according to claim 1, characterized in that: The box body (1) and the cover body (2) are both dark-colored box bodies and dark-colored cover bodies.
7. The cell culture device according to any one of claims 1 to 6, characterized in that: Shock-absorbing chambers (10) are respectively arranged at the four corners of the bottom of the box body (1), and a magnetic suspension shock absorber (11) is arranged in the shock-absorbing chamber (10), and the magnetic suspension shock absorber (11) is exposed at the bottom of the box body (1).
8. The cell culture device according to claim 7, characterized in that: The magnetic suspension shock absorber (11) comprises a magnetic suspension shock absorber cylinder (1101) and a first magnet (1102) and a second magnet (1103) located at the top of the magnetic suspension shock absorber cylinder (1101); the magnetic suspension shock absorber cylinder (1101) is exposed at the bottom of the box body (1), and the magnetic poles of the first magnet (1102) and the second magnet (1103) are arranged opposite to each other.