Small molecule polypeptide separation device

Abstract

A centrifugal mechanism comprises a central gear and a gear set, the central gear is arranged below an upper plate block, the gear set is composed of a first gear, a second gear and a third gear, the gear set is meshed with the central gear, positioning holes are symmetrically formed in the surface of the gear set in a clamped mode, a sleeve is arranged in the gear set in a sleeved mode, and the gear set is arranged in the sleeve. The sleeve is installed at the bottom end of the feeding head in a threaded meshing mode, the positioning column is matched with the positioning hole, the gear set can drive the separation column to rotate and separate polypeptide of the same type at the same time, a filter membrane is arranged on the inner wall of the separation column, a plurality of small holes are distributed in the filter membrane in a surrounding mode, and centrifugal force is generated in the separation column rotating at a high speed. The raw materials in the separation column pass through the micropores in the filter membrane and are thrown out of the micropores, so that the effect of separating small-molecule polypeptide is achieved.

Description

Technical Field

[0001] The utility model belongs to the technical field of polypeptide separation, and in particular relates to a small molecule polypeptide separation device. Background Art

[0002] In polypeptide production, membrane separation technology is often used to separate and purify polypeptide raw materials. Membrane separation technology refers to the technology of achieving selective separation of mixtures of molecules of different particle sizes at the molecular level when they pass through a semipermeable membrane. Semipermeable membranes are also called separation membranes or filter membranes. The membrane wall is covered with small holes. According to the pore size, they can be divided into: microfiltration membrane (MF), ultrafiltration membrane (UF), nanofiltration membrane (NF), reverse osmosis membrane (RO), etc.

[0003] With reference to Chinese Patent 202022442969.8, a separation device for small molecule polypeptides comprises a support plate, a connecting rod, a feeding rod, a separation tank, a cover plate, a separation column, a limiting mechanism and a driving mechanism, wherein the top of the support plate is fixedly connected to the bottom of the connecting rod, and one side of the top of the connecting rod is slidably connected to the feeding rod, the feeding rod is vertically arranged, the separation tank is arranged below the feeding rod, the separation tank is provided with a cavity with an opening facing upward, the opening end surface of the separation tank is detachably connected to the cover plate, the cover plate is provided with a sliding hole adapted to the feeding rod, the separation column is arranged in the cavity, the separation column is provided with a separation chamber with an opening facing upward, the bottom of the feeding rod passes through the sliding hole and is arranged in the separation chamber, the inner wall of the separation column is provided with a separation membrane, the side of the separation column is provided with a separation hole, the feed rod is provided with a feeding hole connected to the separation chamber, the bottom of the separation tank is rotatably connected to the support plate, and the limiting mechanism is used to enable the separation tank to drive the separation column to rotate.

[0004] The above patent only uses one separation column to separate polypeptides, which undoubtedly increases the workload of the separation column, slows down the separation process, and reduces the separation effect. In addition, the polypeptide separation device can only be used for one polypeptide separation function, and cannot perform separation operations on different types of polypeptides at the same time, resulting in poor functional effects. Utility Model Content

[0005] The utility model aims to provide a small molecule polypeptide separation device to solve the problems of poor functional effect and separation burden in the prior art.

[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0007] A small molecule polypeptide separation device comprises an upper plate, a middle plate and a lower plate, and also comprises a centrifugal mechanism installed on the upper plate, wherein three feed heads are evenly distributed on the surface of the upper plate;

[0008] The centrifugal mechanism comprises a central gear and a gear set, wherein the central gear is below the upper plate, the gear set is composed of a first gear, a second gear and a third gear, the gear set and the central gear are meshed with each other, the surface of the gear set is symmetrically provided with positioning holes, a sleeve is sleeved inside the gear set, and the sleeve is threadedly meshed and installed at the bottom of the feed head;

[0009] A separation column is arranged below the gear set.

[0010] A plurality of dividing plates are fixed between the middle plate and the lower plate.

[0011] Furthermore, a liquid inlet head is fixed at one end of the separation column and is connected to the interior of the separation column. Positioning columns are symmetrically arranged on both sides of the liquid inlet head. The liquid inlet head cooperates with the sleeve, and the positioning columns cooperate with the positioning holes. A support column is provided at the bottom of the separation column, and the support column is rotatably installed in the lower plate.

[0012] Furthermore, a filter membrane is provided on the inner wall of the separation column, and a plurality of small holes are distributed around the filter membrane. The high-speed rotating separation column generates centrifugal force inside, so that the raw materials in the separation column pass through the micropores on the filter membrane and are thrown out from the micropores, thereby separating small molecule polypeptides.

[0013] Furthermore, the partition plates are provided with three pieces to divide the space of the middle plate and the lower plate into three groups of spaces. The outer shell, the middle plate and the lower plate constitute a sealed space, and when the three separation columns rotate rapidly, the polypeptides separated by centrifugation are kept in the sealed space for temporary storage, and the polypeptides separated by the three separation columns are concentrated in the sealed space.

[0014] Furthermore, the three partition plates are distributed around 120°, and the outer walls of the upper plate, the middle plate and the lower plate are sealed with a shell. The partition plates distributed around 120° can make the internal space of the device evenly arranged.

[0015] Furthermore, the lower plate has three discharge ports equidistantly distributed. The separated polypeptides are discharged through the discharge ports, and only a receiving container needs to be placed below the discharge ports or the polypeptides can be directly transported through pipelines.

[0016] The technical solution of the utility model has the following beneficial effects:

[0017] 1. The positioning column and the positioning hole cooperate with each other. The drive of the gear set can simultaneously drive the separation column to rotate and separate the same type of polypeptides. A filter membrane is arranged on the inner wall of the separation column. A number of small holes are distributed around the filter membrane. The centrifugal force is generated inside the high-speed rotating separation column, so that the raw materials in the separation column pass through the micropores on the filter membrane and are thrown out from the micropores, thereby separating small molecule polypeptides.

[0018] 2. The setting of the gear set drives multiple separation columns to rotate at the same time, which can reduce the separation burden of a single separation column and improve the separation effect. At the same time, it can also simultaneously separate multiple types of polypeptides and improve the universal adaptability of the equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings required for describing the embodiments are briefly introduced below.

[0020] Figure 1 It is a schematic diagram of the overall structure of the utility model.

[0021] Figure 2 It is a schematic diagram of the overall internal structure of the utility model.

[0022] Figure 3 It is a schematic diagram of the partial split structure of the utility model.

[0023] Figure 4 This is a schematic diagram of the gear set structure of the utility model.

[0024] Figure 5 It is a schematic diagram of the cross-sectional structure of the utility model.

[0025] Figure 6 It is a schematic diagram of the cross-sectional structure of the utility model.

[0026] Figure 7 It is a schematic diagram of the partial cross-sectional structure of the utility model.

[0027] Attached figures: 10, upper plate; 11, motor; 12, center gear; 13, first gear; 14, second gear; 15, third gear; 16, positioning hole; 17, separation column; 18, liquid inlet head; 19, positioning column; 20, support column; 21, middle plate; 22, lower plate; 23, dividing plate; 24, discharge port; 25, feed head; 26, sleeve; 27, outer shell. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical solution and advantages of the utility model clearer, the utility model will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the utility model and are not used to limit the utility model. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.

[0029] Embodiment 1:

[0030] refer to Figure 1-Figure 7, a small molecule polypeptide separation device, comprising an upper plate 10, a middle plate 21 and a lower plate 22, and also comprising a centrifugal mechanism installed on the upper plate 10, and three feed heads 25 are evenly distributed on the surface of the upper plate 10;

[0031] The centrifugal mechanism includes a central gear 12 and a gear set. A motor 11 is fixed on the top of the upper plate 10. The output end of the motor 11 is connected to the central gear 12. The central gear 12 is below the upper plate 10. The gear set consists of a first gear 13, a second gear 14 and a third gear 15. The gear set and the central gear 12 are meshed with each other. Positioning holes 16 are symmetrically provided on the surface of the gear set. A sleeve 26 is provided inside the gear set. The sleeve 26 is threadedly meshed and installed at the bottom of the feed head 25.

[0032] In the above scheme, the polypeptide to be separated is added inside the feed head 25, and multiple feed heads 25 can be used for adding multiple polypeptides. A liquid inlet head 18 is fixed at one end of the separation column 17 and is connected to the inside of the separation column 17. The polypeptide entering from the feed head 25 will enter the separation column 17 from the liquid inlet head 18. When the motor 11 starts, the central gear 12 will synchronously drive the first gear 13, the second gear 14 and the third gear 15 to rotate. There are symmetrical positioning columns 19 on both sides of the liquid inlet head 18. The liquid inlet head 18 cooperates with the sleeve 26, and the positioning column 19 cooperates with the positioning hole 16. A support column 20 is provided at the bottom of the separation column 17, wherein the support column 20 is rotatably mounted on In the lower plate 22, a separation column 17 is installed below the gear group. Since the positioning column 19 cooperates with the positioning hole 16, the drive of the gear group can simultaneously drive the separation column 17 to rotate. A filter membrane is provided on the inner wall of the separation column 17, and a plurality of small holes are distributed around the filter membrane. Centrifugal force is generated inside the high-speed rotating separation column 17, so that the raw materials in the separation column 17 are thrown out from the micropores through the micropores on the filter membrane, thereby separating small molecule polypeptides. By setting up the gear group to simultaneously drive multiple separation columns 17 to rotate, the separation burden of a single separation column 17 can be reduced, and the separation effect can be improved. At the same time, multiple types of polypeptides can be separated synchronously, thereby improving the universal adaptability of the equipment.

[0033] Furthermore, the upper end of the feed head 25 is a conical opening, and the top diameter of the conical opening structure is larger than its bottom diameter. The conical opening can facilitate the addition of polypeptide solution to the interior, and a pipe can also be passed deep into the liquid inlet head 18 to directly discharge the solution into the separation column 17 for separation. The selected pipe can be compatible with the diameter of the liquid inlet head 18, so that when the separation column 17 rotates at a high speed, the internal polypeptide can be prevented from being splashed out of the feed head 25 due to the centrifugal force. The diameter of the liquid inlet head 18 is much smaller than the diameter of the separation column 17, which can prevent the polypeptide from being splashed out of the feed head 25 due to the centrifugal force.

[0034] In this embodiment: It is worth noting that the outer walls of the upper plate 10, the middle plate 21 and the lower plate 22 are sealed with a shell 27, and the shell 27, the middle plate 21 and the lower plate 22 constitute a sealed space. When the three separation columns 17 rotate rapidly, the polypeptides separated by centrifugation are kept in the sealed space for temporary storage, and the polypeptides separated by the three separation columns 17 are concentrated in the sealed space. The mixing method is applicable to the same polypeptide, and during the centrifugal rotation process, the polypeptide to be separated can be directly added through the feed head 25, and can be directly added without stopping the instrument during separation, saving the time required for operation.

[0035] Embodiment 2:

[0036] refer to Figure 2-Figure 6 A partition plate 23 is fixed between the middle plate 21 and the lower plate 22. Three partition plates 23 are provided to divide the space between the middle plate 21 and the lower plate 22 into three groups of spaces. The three partition plates 23 are arranged in a 120° circle, and the partition plates 23 arranged in a 120° circle can make the internal space of the device evenly arranged. It should be noted that the device is not limited to three groups of spaces and three separation columns 17, and the spaces and separation columns 17 can be appropriately reduced or increased.

[0037] In the above scheme, the partition plate 23 is arranged to divide the internal storage space of the equipment into three, and each space is provided with a separation column 17. When operating, it is only necessary to add the polypeptides to be separated inside the feed head 25. The polypeptide solutions added by each feed head 25 may be different or of different types. In the mode of the second embodiment, different types of polypeptide solutions can be mixed and separated at the same time.

[0038] Furthermore, the lower plate 22 has three equally spaced discharge ports 24. The separated polypeptides are discharged through the discharge ports 24. It is sufficient to place a receiving container under the discharge port 24 or directly transport the polypeptides through a pipeline. The bottom of the lower plate 22 is provided with supporting feet for supporting on the ground, so that there is a gap between the ground and the lower plate 22, so that it is convenient to place a container to receive the separated polypeptides.

[0039] The specific implementation process of this embodiment is as follows:

[0040] When the dividing plate 23 is not provided: the polypeptides to be separated are added into the feed head 25, and multiple feed heads 25 can be used for adding multiple polypeptides. A liquid inlet head 18 is fixed at one end of the separation column 17 and is connected to the inside of the separation column 17. The polypeptides entering from the feed head 25 will enter the separation column 17 from the liquid inlet head 18. When the motor 11 starts, the central gear 12 will synchronously drive the first gear 13, the second gear 14 and the third gear 15 to rotate. The positioning column 19 cooperates with the positioning hole 16. The drive of the gear set can simultaneously drive the separation column 17 to rotate. A filter membrane is provided on the inner wall of the separation column 17. A plurality of small holes are distributed around the filter membrane. Centrifugal force is generated inside the high-speed rotating separation column 17, so that the raw materials in the separation column 17 are thrown out from the micropores through the micropores on the filter membrane, thereby separating small molecule polypeptides. The polypeptides separated by centrifugation are kept in a sealed space for temporary storage, and the polypeptides separated by the three separation columns 17 are concentrated in the sealed space.

[0041] When the partition plate 23 is provided, the internal storage space of the device is divided into three, and each space is provided with a separation column 17. When operating, it is only necessary to add the polypeptides to be separated into the feed head 25. The polypeptide solutions added to each feed head 25 may be different or of different types. The separation column 17 is also driven by the gear set to rotate, thereby achieving the purpose of separating polypeptides.

[0042] The above embodiments are only exemplary embodiments of the present invention and are not intended to limit the present invention. The protection scope of the present invention is defined by the claims. Various modifications or equivalent substitutions may be made to the present invention within the essence and protection scope of the present invention. Such modifications or equivalent substitutions shall also be deemed to fall within the protection scope of the present invention.

[0043] In the description of the present utility model, it should be noted that the terms "inside", "front", "back", "left", "right" and the like indicate directions or positional relationships based on the directions or positional relationships shown in the attached circles, or the directions or positional relationships in which the utility model product is usually placed when in use, and are only used to facilitate the description of the present utility model and simplify the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, these terms indicating directions or positional relationships cannot be understood as limiting the present utility model.

[0044] In the description of the present invention, it is further explained that, unless otherwise clearly specified and limited, the terms "disposed" and "connected" should be understood in a broad sense. For example, these terms can indicate a fixed connection, a detachable connection, or an integral connection between elements; they can also indicate a mechanical connection, an electrical connection; they can also indicate a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of these terms in the present invention can be understood according to specific circumstances.

Claims

1. A small molecule polypeptide separation device, comprising an upper plate (10), a middle plate (21) and a lower plate (22), characterized in that: It also includes a centrifugal mechanism installed on the upper plate (10), and three feed heads (25) are evenly distributed on the surface of the upper plate (10); The centrifugal mechanism comprises a central gear (12) and a gear set, wherein the central gear (12) is located below the upper plate (10), and the gear set is composed of a first gear (13), a second gear (14) and a third gear (15), and the gear set is meshed with the central gear (12), and positioning holes (16) are symmetrically arranged on the surface of the gear set, and a sleeve (26) is sleeved inside the gear set, and the sleeve (26) is threadedly mounted on the bottom end of the feed head (25); A separation column (17) is installed below the gear set; A plurality of partition plates (23) are fixed between the middle plate (21) and the lower plate (22).

2. A small molecule polypeptide separation device according to claim 1, characterized in that: A liquid inlet head (18) is fixed at one end of the separation column (17) and is connected to the inside of the separation column (17). Positioning columns (19) are symmetrically arranged on both sides of the liquid inlet head (18). The liquid inlet head (18) cooperates with the sleeve (26), and the positioning column (19) cooperates with the positioning hole (16). A support column (20) is arranged at the bottom of the separation column (17), wherein the support column (20) is rotatably installed in the lower plate (22).

3. A small molecule polypeptide separation device according to claim 2, characterized in that: The inner wall of the separation column (17) is provided with a filter membrane, and a plurality of small holes are distributed around the filter membrane.

4. A small molecule polypeptide separation device according to claim 3, characterized in that: The dividing plates (23) are provided with three pieces to divide the space of the middle plate (21) and the lower plate (22) into three groups of spaces.

5. A small molecule polypeptide separation device according to claim 4, characterized in that: The three partition plates (23) are distributed in a circumferential manner at an angle of (120) degrees, and the outer walls of the upper plate (10), the middle plate (21) and the lower plate (22) are sealed with an outer shell (27).

6. A small molecule polypeptide separation device according to claim 5, characterized in that: The lower plate (22) has three discharge openings (24) which are evenly distributed.

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