Full-automatic nucleic acid extractor

Abstract

The utility model relates to a full-automatic nucleic acid extractor. According to the full-automatic nucleic acid extraction instrument, manual nucleic acid extraction is replaced by automatic nucleic acid extraction through overall design, and efficiency is improved; compared with a foreign large full-automatic nucleic acid extraction instrument, the miniaturized automatic nucleic acid extraction instrument has the advantages that the cost can be effectively reduced, and the popularization rate of equipment can be favorably realized; nucleic acid extraction work such as suction and discharge of reagents can be realized through an automatic pipetting arm and a manipulator which are independently designed; the heating and mixing of the nucleic acid extraction mixed reagent can be realized through the independently designed heating and uniformly mixing assembly, and the nucleic acid extraction efficiency is improved. And through the designed cleaning tank, the tips pipe can be recycled, and the efficiency is improved.

Description

Technical Field

[0001] The present application relates to the technical field of nucleic acid extraction instruments, and in particular to a fully automatic nucleic acid extraction instrument. Background Art

[0002] The nucleic acid extractor is an instrument that uses matching nucleic acid extraction reagents to automatically complete the sample nucleic acid extraction work. It is widely used in various fields such as disease control centers, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbiology testing, food safety testing, animal husbandry and molecular biology research.

[0003] Traditional methods of nucleic acid extraction include the following three methods: manual extraction, large-scale automated nucleic acid extraction instrument extraction, and small-scale automated nucleic acid extraction instrument extraction. The three methods have the following defects:

[0004] (1) Manual extraction: The samples need to be extracted manually, and only a single sample can be extracted each time, which makes the efficiency very low. In addition, the instability of the operation during manual extraction leads to inaccurate extraction data.

[0005] (2) Through large-scale automated nucleic acid extraction instruments: This type of equipment is expensive and often requires the use of matching consumables, which are expensive. Therefore, although it is efficient, the cost is very high.

[0006] (3) Through small-scale automated nucleic acid extraction instrument: The degree of integration is low, fewer samples are processed each time, and the efficiency is low. In addition, the quality of domestic products is currently unstable, resulting in unstable nucleic acid extraction accuracy. Summary of the invention

[0007] Based on this, it is necessary to provide a fully automatic nucleic acid extractor.

[0008] The present application is implemented through the following scheme. The present application provides a fully automatic nucleic acid extraction instrument, including:

[0009] A base, on which a guide rail mounting frame, a gun tip placement frame, a reagent tank mounting frame, a cleaning tank, a garbage collector, a reagent kit mounting frame and a heating and mixing component are arranged;

[0010] The guide rail mounting frame is provided with a guide rail, a left cantilever, a right cantilever, a first driving assembly, a second driving assembly, an automatic pipetting arm and a reagent kit grabbing manipulator, wherein the first driving assembly is connected to the left cantilever and is used to drive the left cantilever to slide horizontally left and right along the guide rail; the second driving assembly is connected to the right cantilever and is used to drive the right cantilever to slide horizontally left and right along the guide rail;

[0011] The left cantilever is provided with a third driving component, and the third driving component is connected to the automatic pipetting arm, and is used for driving the automatic pipetting arm to move forward and backward in the horizontal direction along the left cantilever, so that the automatic pipetting arm moves above the gun tip placement rack, the reagent trough mounting rack, the cleaning trough, the garbage collector, and the reagent reagent mounting rack; the right cantilever is provided with a fourth driving component, and the fourth driving component is connected to the reagent reagent grabbing robot, and is used for driving the reagent reagent grabbing robot to move forward and backward in the horizontal direction along the right cantilever, so that the reagent reagent grabbing robot moves above the reagent reagent mounting rack and the heating and mixing component.

[0012] In one embodiment, the guide rail mounting frame includes a back plate and a left plate and a right plate arranged on both sides of the back plate, a guide rail mounting plate is arranged between the left plate and the right plate, and the guide rail is mounted on the guide rail mounting plate;

[0013] The first driving assembly includes a first motor, a first coupling, a first screw rod and a first connecting block; the first motor is fixed to the left side plate, the first coupling connects the first motor and the first screw rod, and the first screw rod is arranged parallel to the guide rail; the first connecting block is sleeved on the first screw rod, and the first connecting block is fixedly connected to the left cantilever;

[0014] The second driving assembly includes a second motor, a second coupling, a second screw rod and a second connecting block; the second motor is fixed to the left side plate, the second coupling connects the second motor and the second screw rod, and the second screw rod is arranged parallel to the first screw rod; the second connecting block is sleeved on the second screw rod, and the second connecting block is fixedly connected to the right cantilever.

[0015] In one embodiment, the left cantilever is provided with a first through hole and a second through hole, and the right cantilever is provided with a third through hole and a fourth through hole;

[0016] The first screw rod is connected to the right side plate after passing through the first through hole and the third through hole; the second screw rod is connected to the right side plate after passing through the second through hole and the fourth through hole.

[0017] In one embodiment, the left cantilever includes a first shell, and the third drive assembly is disposed in the first shell; the right cantilever includes a second shell, and the fourth drive assembly is disposed in the second shell;

[0018] The third driving assembly includes a third motor, a third coupling, a third screw and a third connecting block, wherein the third motor is fixed in the first housing, the third coupling connects the third motor and the third screw, and the third screw is horizontally arranged in a direction perpendicular to the guide rail; the third connecting block is sleeved on the third screw, and the third connecting block is fixedly connected to the automatic pipetting arm;

[0019] The fourth drive assembly includes a fourth motor, a fourth coupling, a fourth screw rod and a fourth connecting block. The fourth motor is fixed in the second housing. The fourth coupling connects the fourth motor and the fourth screw rod. The fourth screw rod is arranged parallel to the third screw rod. The fourth connecting block is sleeved on the fourth screw rod, and the fourth connecting block is fixedly connected to the reagent kit grabbing robot.

[0020] In one embodiment, the automatic pipetting arm comprises a left connecting block, a left mounting plate, and a left up and down motor, a left lifting screw, a left vertical guide rail, a left connecting seat and an automatic pipetting assembly mounted on the left mounting plate; the left mounting plate is vertically arranged and fixedly connected to the third connecting block through the left connecting block;

[0021] The left lifting screw is vertically arranged and connected to the left up-and-down motor, and a left lifting nut is sleeved on the left lifting screw;

[0022] The left connecting seat is slidably connected to the left vertical guide rail via a left sliding block, and the left lifting nut is fixedly connected to the left connecting seat;

[0023] The automatic pipetting assembly is fixedly connected to the left connecting seat, and is used to take the pipette tip placed on the tip placement rack; absorb the reagent placed on the reagent tank mounting rack, and inject the absorbed reagent into the reagent reagent kit on the reagent kit mounting rack through the injection mechanism in the automatic pipetting assembly.

[0024] In one of the embodiments, the reagent kit grabbing manipulator includes a right connecting block, a right mounting plate, and a right up-and-down motor, a right lifting screw, a right vertical guide rail, a rotary motor mounting seat, a rotary motor, a fifth coupling, a mechanical finger mounting seat, a mechanical finger and a gripping hand assembly mounted on the right mounting plate;

[0025] The right mounting plate is vertically arranged and fixedly connected to the fourth connecting block through the right connecting block;

[0026] The right lifting screw is vertically arranged and connected to the right up-and-down motor, and a right lifting nut is sleeved on the right lifting screw;

[0027] The rotating motor mounting seat is slidably connected to the right vertical guide rail via a right slider, and the right lifting nut is fixedly connected to the rotating motor mounting seat;

[0028] The rotating motor is mounted on the rotating motor mounting seat, the robotic finger mounting seat is connected to the rotating motor via the fifth coupling, the robotic finger is mounted on the robotic finger mounting seat, and the clamping hand assembly is mounted on the robotic finger; the robotic finger is used to control the opening and closing distance of the clamping hand assembly according to the size of the reagent kit on the reagent kit mounting rack to achieve clamping and placement of the reagent kit.

[0029] In one of the embodiments, the right up-down motor is mounted on the right mounting plate via an up-down motor mounting seat;

[0030] The right up-and-down motor is provided with a first positioning sensor, and the rotating motor mounting seat is provided with a first limiting piece. The first positioning sensor is used to detect whether the first limiting piece rises to a specified position.

[0031] In one of the embodiments, a second positioning sensor is disposed on the robot finger mounting seat, and a second limiting plate is disposed on the fifth coupling, and the second positioning sensor is used to detect whether the second limiting plate is rotated to a specified position.

[0032] In one embodiment, the heating and mixing assembly includes a mounting base, a vibration motor, an eccentric bearing, a vibration element, a heating base, a heat sink and a fan;

[0033] The vibration motor is mounted on the mounting base, and its rotating shaft is arranged vertically upward; the eccentric bearing is sleeved on the rotating shaft; the vibrating member is sleeved on the eccentric bearing to generate vibration on a horizontal plane driven by the vibrating member; an eccentric flyweight is also eccentrically sleeved on the eccentric bearing;

[0034] The heating base is installed above the vibrating member and is fixedly connected to the vibrating member; the heat sink is arranged below the heating base, and the fan is arranged on the side of the heat sink.

[0035] In one embodiment, the cleaning tank includes a tank body, in which an outer wastewater tank and an inner cleaning tank are formed, and the tank wall height of the inner cleaning tank is lower than the tank wall height of the tank body; a drain outlet is provided at the bottom of the outer wastewater tank, and a water inlet is provided at the bottom of the inner cleaning tank.

[0036] The fully automatic nucleic acid extractor of the embodiment of the present application realizes automated nucleic acid extraction to replace manual nucleic acid extraction through overall design, thereby improving efficiency; the miniaturized automatic nucleic acid extractor of this design can effectively reduce costs compared with large-scale fully automatic nucleic acid extractors abroad, which is conducive to achieving the popularity of the equipment; the nucleic acid extraction work such as the absorption and discharge of reagents can be realized through the independently designed automatic pipetting arm and manipulator; the heating and mixing components of the independently designed nucleic acid extraction can realize the heating and mixing of the mixed reagents, thereby improving the efficiency of nucleic acid extraction. Through the designed cleaning tank, the tips tubes can be recycled to improve efficiency. By laying out the operating desktop of the fully automatic small nucleic acid extractor and reasonably arranging the installation positions of the gun head mounting frame, the reagent tank mounting frame, the reagent reagent mounting frame and the garbage collector, the use efficiency of the automatic small nucleic acid extractor is greatly improved, thereby improving the efficiency of nucleic acid extraction. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 This is a schematic diagram of the structure of a fully automatic nucleic acid extractor in one embodiment of the present application;

[0038] Figure 2 and Figure 3 This is a schematic diagram of the structure of a left-handed arm and an automatic pipetting arm in one embodiment of the present application;

[0039] Figure 4-Figure 6 This is a schematic diagram of the structure of the right-handed arm and the reagent kit grabbing manipulator in one embodiment of the present application;

[0040] Figures 7 and 8 This is a schematic diagram of the structure of a heating and mixing component in one embodiment of the present application;

[0041] Fig. 9 This is a cross-sectional view of a heating and mixing component in one embodiment of the present application;

[0042] Fig.10 and Fig.11 Schematic diagram of the structure of a cleaning tank in one embodiment of the present application.

[0043] Description of Figure Numbers:

[0044] 1000, fully automatic nucleic acid extractor; 100, base; 200, guide rail mounting frame; 201, back plate; 202, left side plate; 203, right side plate; 204, guide rail mounting plate; 300, gun head placement rack; 400, reagent tank mounting frame; 500, cleaning tank; 501, tank body; 502, external waste water tank; 503, internal cleaning tank; 504, drain outlet; 505, water inlet; 600, garbage collector; 700, reagent kit mounting frame; 800, heating and mixing component; 801, mounting base; 802, vibration motor; 803, eccentric bearing; 804, vibration 805, heating base; 806, heat sink; 807, fan; 808, eccentric flyweight; 901, guide rail; 902, left cantilever; 9021, first through hole; 9022, second through hole; 9023, first shell; 90241, third motor; 90242, third coupling; 90243, third screw rod; 90244, third connecting block; 903, right cantilever; 9031, third through hole; 9033, second shell; 90341, fourth motor; 90342, fourth coupling; 90343, fourth screw rod; 90344, fourth Connecting block; 9041, first motor; 9042, first coupling; 9043, first screw rod; 9044, first connecting block; 9051, second motor; 9052, second coupling; 9053, second screw rod; 9054, second connecting block; 906, automatic pipetting arm; 9061, left connecting block; 9062, left mounting plate; 9063, left up and down motor; 9064, left lifting screw rod; 9065, left vertical guide rail; 9066, left connecting seat; 9067, automatic pipetting assembly; 9068, left lifting nut; 9069, left slider; 907, reagent Box grabbing robot; 9071, right connecting block; 9072, right mounting plate; 9073, right up and down motor; 9074, right lifting screw; 9075, right vertical guide rail; 9076, rotating motor mounting seat; 9077, rotating motor; 9078, fifth coupling; 9079, mechanical finger mounting seat; 9080, mechanical finger; 9081, clamping hand assembly; 9082, right lifting nut; 9084, up and down motor mounting seat; 9085, first positioning sensor; 9086, first limit plate; 9087, second positioning sensor; 9088, second limit plate. DETAILED DESCRIPTION

[0045] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present application. However, the present application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present application, so the present application is not limited by the specific embodiments disclosed below.

[0046] In the description of the present application, it should be understood that if the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. appear, the orientation or position relationship indicated by these terms is based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application.

[0047] In addition, if the terms "first" or "second" appear, these terms are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of this application, if the term "plurality" appears, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0048] In this application, unless otherwise clearly specified and limited, if the terms "installed", "connected", "connected", "fixed" and the like appear, these terms should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integrated connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to the specific circumstances.

[0049] In the present application, unless otherwise clearly specified and limited, if there is a description that a first feature is "above" or "below" a second feature, etc., or similar descriptions appear, it may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "above" and "above" the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. The first feature being "below", "below" and "below" the second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0050] See also Figure 1The embodiment of the present application provides a fully automatic nucleic acid extractor, including a base 100, on which a guide rail mounting frame 200, a gun head placement frame 300, a reagent tank mounting frame 400, a cleaning tank 500, a garbage collector 600, a reagent reagent mounting frame 700 and a heating and mixing component 800 are arranged. Among them, the gun head placement frame 300 is used to place the liquid pipette gun head, that is, the tips pipette to be used, which can simultaneously place the gun heads of an 8*12 array. In this embodiment, preferably, the gun head placement frame includes two, so that multiple tips pipettes can be used at the same time. The reagent tank mounting frame 400 is used to place the reagent tank. In this embodiment, the reagent tank includes multiple and is used to place a variety of different reaction reagents. The cleaning tank 500 is used to provide running water for cleaning the tips pipette and to remove waste water after cleaning the tips pipette. The garbage collector 600 is used to collect discarded tips pipettes. The reagent reagent mounting frame 700 is used to place the reagent reagent containing the mixed reagent, and the heating and mixing component 800 can achieve heating and mixing of the mixed reagent.

[0051] Specifically, Figure 1-5 As shown, the guide rail mounting frame 200 is provided with a guide rail 901, a left cantilever 902, a right cantilever 903, a first drive assembly, a second drive assembly, an automatic liquid transfer arm 906 and a reagent kit grabbing manipulator 907, the first drive assembly is connected to the left cantilever 902, and is used to drive the left cantilever 902 to slide horizontally left and right along the guide rail; the second drive assembly is connected to the right cantilever 903, and is used to drive the right cantilever 903 to slide horizontally left and right along the guide rail. The left cantilever 902 is provided with a third drive assembly, and the third drive assembly is connected to the automatic liquid transfer arm 906, and is used to drive the automatic liquid transfer arm 906 to move forward and backward along the left cantilever 902 in the horizontal direction, so that the automatic liquid transfer arm 906 moves above the gun head placement frame 300, the reagent tank mounting frame 400, the cleaning tank 500, the garbage collector 600, and the reagent kit mounting frame 700. The right cantilever 903 is provided with a fourth driving assembly, which is connected to the reagent kit grabbing robot 907 and is used to drive the reagent kit grabbing robot 907 to move back and forth in the horizontal direction along the right cantilever 903 so that the reagent kit grabbing robot 907 moves above the reagent kit mounting frame 700 and the heating and mixing assembly 800.

[0052] The working process of this embodiment is as follows: the first driving component drives the left cantilever 902 to move left and right in the horizontal direction, so as to drive the automatic liquid transfer arm 906 to move left and right in the horizontal direction, and the third driving component arranged in the left rotating arm 902 drives the automatic liquid transfer arm 906 to move forward and backward in the horizontal direction, so as to realize the position movement of the automatic liquid transfer arm 906 in the XY plane. The automatic liquid transfer arm 906 is driven to move to the top of the gun head placement rack 300, and to take the liquid suction gun head placed on the gun head placement rack 300; after taking the liquid suction gun head, the automatic liquid transfer arm 906 is moved to the top of the reagent tank mounting rack 400, and the reagent placed on the reagent tank mounting rack 400 is sucked as required; after sucking the reagent, the automatic liquid transfer arm 906 is continued to be moved, and the sucked reagent is injected into the reagent kit on the reagent kit mounting rack 700 through the injection mechanism in the automatic liquid transfer arm 906. Repeat the above operation, and inject the required multiple reagents into the reagent kit on the reagent kit mounting rack 700. Finally, the required mixed reagent is obtained. In the above operation, if multiple reagents are injected, each reagent needs to be aspirated using the automatic pipetting arm 906 to avoid the problem of reagent mixing and contamination. Therefore, after the first reagent is aspirated, when the second reagent is aspirated, the pipette tip on the automatic pipetting arm 906 needs to be cleaned. During the cleaning, the automatic pipetting arm 906 needs to be controlled to move into the cleaning tank 500 to clean the pipette tip, and then the second reagent is aspirated after cleaning.

[0053] The second driving assembly drives the right cantilever 903 to move horizontally to the left and right, so as to drive the reagent kit grabbing manipulator 907 to move horizontally to the left and right, and the fourth driving assembly provided in the right cantilever 903 drives the reagent kit grabbing manipulator 907 to move horizontally forward and backward, so as to realize the position movement of the reagent kit grabbing manipulator 907 in the XY plane. Under the drive, the reagent kit grabbing manipulator 907 clamps the reagent kit containing the mixed reagent from the reagent kit mounting frame 700, places it in the heating mixer assembly 800 for mixing and heating, and after mixing evenly, takes out the reagent kit for subsequent operations.

[0054] The fully automatic nucleic acid extractor of the embodiment of the present application realizes automated nucleic acid extraction to replace manual nucleic acid extraction through overall design, thereby improving efficiency; the miniaturized automatic nucleic acid extractor of this design can effectively reduce costs compared with large-scale fully automatic nucleic acid extractors abroad, which is conducive to achieving the popularity of the equipment; the nucleic acid extraction work such as the absorption and discharge of reagents can be realized through the independently designed automatic pipetting arm and manipulator; the heating and mixing components of the independently designed nucleic acid extraction can realize the heating and mixing of the mixed reagents, thereby improving the efficiency of nucleic acid extraction. Through the designed cleaning tank, the tips tubes can be recycled to improve efficiency. By laying out the operating desktop of the fully automatic small nucleic acid extractor and reasonably arranging the installation positions of the gun head mounting frame, the reagent tank mounting frame, the reagent reagent mounting frame and the garbage collector, the use efficiency of the automatic small nucleic acid extractor is greatly improved, thereby improving the efficiency of nucleic acid extraction.

[0055] In one embodiment, Figure 1-Figure 5 As shown, the guide rail mounting frame 200 includes a back plate 201 and a left side plate 202 and a right side plate 203 arranged on both sides of the back plate 201, a guide rail mounting plate 204 is arranged between the left side plate 202 and the right side plate 203, and the guide rail is installed on the guide rail mounting plate 204; the first driving component includes a first motor 9041, a first coupling 9042, a first screw rod 9043 and a first connecting block 9044; the first motor 9041 is fixed on the left side plate 202, the first coupling 9042 connects the first motor 9041 and the first screw rod 9043, and the first screw rod 9043 is arranged parallel to the guide rail; the first connecting block 9044 is sleeved on the first screw rod 9043, and the first connecting block 9044 is fixedly connected to the left cantilever 902.

[0056] The second driving assembly includes a second motor 9051, a second coupling 9052, a second screw rod 9053 and a second connecting block 9054; the second motor 9051 is fixed on the left side plate 202, the second coupling 9052 connects the second motor 9051 and the second screw rod 9053, and the second screw rod 9053 is arranged parallel to the first screw rod 9043; the second connecting block 9054 is sleeved on the second screw rod 9053, and the second connecting block 9054 is fixedly connected to the right cantilever 903.

[0057] Preferably, Figure 1-5 As shown, the left cantilever 902 is provided with a first through hole 9021 and a second through hole 9022, and the right cantilever 903 is provided with a third through hole 9031 and a fourth through hole (not shown);

[0058] The first screw rod 9043 passes through the first through hole 9021 and the third through hole 9031 and is connected to the right side plate 203 ; the second screw rod 9053 passes through the second through hole 9022 and the fourth through hole 9032 and is connected to the right side plate 203 .

[0059] In one embodiment, if Figure 2-6 As shown, the left cantilever 902 includes a first shell 9023 , and the third driving assembly is disposed in the first shell 9023 ; the right cantilever 903 includes a second shell 9033 , and the fourth driving assembly is disposed in the second shell 9033 .

[0060] The third driving assembly includes a third motor 90241, a third coupling 90242, a third screw rod 90243 and a third connecting block 90244. The third motor 90241 is fixed in the first shell 9023. The third coupling 90242 connects the third motor 90241 and the third screw rod 90243. The third screw rod 90243 is horizontally arranged in a direction perpendicular to the guide rail; the third connecting block 90244 is sleeved on the third screw rod 90243, and the third connecting block 90244 is fixedly connected to the automatic pipetting arm 906. The fourth driving assembly includes a fourth motor 90341, a fourth coupling 90342, a fourth screw rod 90343 and a fourth connecting block 90344. The fourth motor 90341 is fixed in the second shell 9033. The fourth coupling 90342 connects the fourth motor 90341 and the fourth screw rod 90343. The fourth screw rod 90343 is arranged parallel to the third screw rod 90243. The fourth connecting block 90344 is sleeved on the fourth screw rod 90343, and the fourth connecting block 90344 is fixedly connected to the reagent box grabbing robot 907.

[0061] Specifically, Figure 2-3 As shown, the automatic pipetting arm 906 includes a left connecting block 9061, a left mounting plate 9062, and a left up and down motor 9063, a left lifting screw 9064, a left vertical guide rail 9065901, a left connecting seat 9066 and an automatic pipetting assembly 9067 installed on the left mounting plate 9062; the left mounting plate 9062 is vertically arranged and fixedly connected to the third connecting block 90244 through the left connecting block 9061. Among them, the left lifting screw 9064 is vertically arranged and connected to the left up and down motor 9063, and a left lifting nut 9068 is sleeved on the left lifting screw 9064; the left connecting seat 9066 is slidingly connected to the left vertical guide rail 9065901 through the left slider 9069, and the left lifting nut 9068 is fixedly connected to the left connecting seat 9066; the automatic pipetting component 9067 is fixedly connected to the left connecting seat 9066, and the automatic pipetting component 9067 is used to take the aspiration gun tip placed on the gun tip placement rack 300; absorb the reagent placed on the reagent tank mounting rack 400, and inject the absorbed reagent into the reagent reagent mounting rack 700 through the injection mechanism in the automatic pipetting component 9067.

[0062] In this embodiment, the specific structure of the automatic pipetting component 9067 can refer to the Chinese utility model patent with publication number CN208407033U, which will not be repeated here.

[0063] like Figure 4-6 As shown, in one embodiment, the test kit grasping robot 907 includes a right connecting block 9071, a right mounting plate 9072, and a right up and down motor 9073, a right lifting screw 9074, a right vertical guide rail 9075, a rotating motor mounting seat 9076, a rotating motor 9077, a fifth coupling 9078, a robotic finger mounting seat 9079, a robotic finger 9080 and a clamping hand assembly 9081 installed on the right mounting plate 9072. The right mounting plate 9072 is vertically arranged and fixedly connected to the fourth connecting block 90344 through the right connecting block 9071; the right lifting screw 9074 is vertically arranged and connected to the right up-and-down motor 9073, and a right lifting nut 9082 is sleeved on the right lifting screw 9074; the mounting seat 9076 of the rotating motor 9077 is slidably connected to the right vertical guide rail 9075901 through the right slider, and the right lifting nut 9082 is fixedly connected to the mounting seat 9076 of the rotating motor 9077; the rotating motor 9077 Installed on the rotating motor 9077 mounting seat 9076, the robotic finger 9080 mounting seat 9079 is connected to the rotating motor 9077 through the fifth coupling 9078, the robotic finger 9080 is installed on the robotic finger 9080 mounting seat 9079, and the clamping hand assembly 9081 is installed on the robotic finger 9080; the robotic finger 9080 is used to control the opening and closing distance of the clamping hand assembly 9081 according to the size of the reagent kit on the reagent kit mounting frame 700, so as to achieve the clamping and placement of the reagent kit.

[0064] Preferably, the kit is a 96-well plate.

[0065] Preferably, Figure 4-6 As shown, the right up-down motor 9073 is mounted on the right mounting plate 9072 through the up-down motor mounting seat 9084; the right up-down motor 9073 is provided with a first positioning sensor 9085, and the rotating motor mounting seat 9076 is provided with a first limiting piece 9086, and the first positioning sensor 9085 is used to detect whether the first limiting piece 9086 rises to a specified position. The mounting seat 9079 of the mechanical finger 9080 is provided with a second positioning sensor 9087, and the fifth coupling 9078 is provided with a second limiting piece 9088, and the second positioning sensor 9087 is used to detect whether the second limiting piece 9088 rotates to a specified position.

[0066] Specifically, the first positioning sensor 9085 includes a transmitter and a receiver. When the first limiting plate 9086 rises to a set height driven by the rotating motor mounting seat 9076, it is used to cut off the signal of the first positioning sensor 9085, thereby realizing the detection and limitation of the height of the rotating motor mounting seat 9076.

[0067] The principle of the second positioning sensor 9087 is similar to that of the first positioning sensor 9085, and it also includes a transmitter and a receiver. When the second limit plate 9088 is driven by the fifth coupling 9078 and rotates to a set angle, it is used to cut off the signal of the second positioning sensor 9087, thereby realizing the detection and limitation of the rotation angle of the fifth coupling 9078, and then realizing the detection and limitation of the rotation angle of the rotating motor 9077.

[0068] like Figures 7 to 9 As shown, the heating and mixing assembly 800 includes a mounting base 801, a vibration motor 802, an eccentric bearing 803, a vibration member 804, a heating base 805, a heat sink 806 and a fan 807. The vibration motor 802 is mounted on the mounting base 801, and its rotation axis is vertically arranged upward; the eccentric bearing 803 is sleeved on its rotation axis. The vibration member 804 is sleeved on the eccentric bearing 803, so that it swings in the XY plane under the drive of the eccentric bearing 803, thereby generating vibration in the horizontal plane.

[0069] Preferably, an eccentric flyweight 808 is eccentrically sleeved on the eccentric bearing 803 ; the eccentric flyweight 808 is used to drive the vibration member 804 to swing.

[0070] The heating base 805 is installed above the vibrating member 804 and is fixedly connected to the vibrating member 804. The heating base 805 is used to install the reagent kit and heat the reagent kit. The heat sink 806 is arranged below the heating base 805. The fan 807 is arranged on the side of the heat sink 806 to increase the ventilation of the heat sink 806, realize the heat dissipation of the heating base 806, and thus adjust the heating temperature of the heating base 806.

[0071] In one embodiment, if Fig.10 and Fig.11 As shown, the cleaning tank 500 includes a tank body 501, in which an external wastewater tank 502 and an internal cleaning tank 503 are formed, and the tank wall height of the internal cleaning tank 503 is lower than the tank wall height of the tank body 501; a drain outlet 504 is provided at the bottom of the external wastewater tank 502, and a water inlet 505 is provided at the bottom of the internal cleaning tank 503.

[0072] During cleaning, the live water for external cleaning enters the inner cleaning tank 503 from the bottom through the water inlet 505. The pipette tip clamped by the automatic pipetting arm 906 needs to be cleaned after absorbing the reagent. After the automatic pipetting arm 906 is controlled to move to the top of the cleaning tank 500, the automatic pipetting arm 906 controls the pipette tip to move downward, and the pipette tip is deeply inserted into the inner cleaning tank 503, and the pipette tip is cleaned by circulating live water. The waste water after cleaning enters the external wastewater tank 502, and then circulates to the outside of the fully automatic nucleic acid extractor 1000 through the drain port 504, thereby realizing the cyclic reciprocating cleaning of the pipette tip.

[0073] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0074] The above-described embodiments only express several implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be construed as limiting the scope of the patent application. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the protection scope of the patent application shall be subject to the attached claims.

Claims

1. A fully automatic nucleic acid extractor, characterized in that: include: A base, on which a guide rail mounting frame, a gun tip placement frame, a reagent tank mounting frame, a cleaning tank, a garbage collector, a reagent kit mounting frame and a heating and mixing component are arranged; The guide rail mounting frame is provided with a guide rail, a left cantilever, a right cantilever, a first driving assembly, a second driving assembly, an automatic pipetting arm and a reagent kit grabbing manipulator, wherein the first driving assembly is connected to the left cantilever and is used to drive the left cantilever to slide horizontally left and right along the guide rail; the second driving assembly is connected to the right cantilever and is used to drive the right cantilever to slide horizontally left and right along the guide rail; The left cantilever is provided with a third driving component, and the third driving component is connected to the automatic pipetting arm, and is used for driving the automatic pipetting arm to move forward and backward in the horizontal direction along the left cantilever, so that the automatic pipetting arm moves above the gun tip placement rack, the reagent trough mounting rack, the cleaning trough, the garbage collector, and the reagent reagent mounting rack; the right cantilever is provided with a fourth driving component, and the fourth driving component is connected to the reagent reagent grabbing robot, and is used for driving the reagent reagent grabbing robot to move forward and backward in the horizontal direction along the right cantilever, so that the reagent reagent grabbing robot moves above the reagent reagent mounting rack and the heating and mixing component.

2. A fully automatic nucleic acid extractor according to claim 1, characterized in that: The guide rail mounting frame comprises a back plate and a left plate and a right plate arranged on both sides of the back plate, a guide rail mounting plate is arranged between the left plate and the right plate, and the guide rail is mounted on the guide rail mounting plate; The first driving assembly includes a first motor, a first coupling, a first screw rod and a first connecting block; the first motor is fixed to the left side plate, the first coupling connects the first motor and the first screw rod, and the first screw rod is arranged parallel to the guide rail; the first connecting block is sleeved on the first screw rod, and the first connecting block is fixedly connected to the left cantilever; The second driving assembly includes a second motor, a second coupling, a second screw rod and a second connecting block; the second motor is fixed to the left side plate, the second coupling connects the second motor and the second screw rod, and the second screw rod is arranged parallel to the first screw rod; the second connecting block is sleeved on the second screw rod, and the second connecting block is fixedly connected to the right cantilever.

3. A fully automatic nucleic acid extractor according to claim 2, characterized in that: The left cantilever is provided with a first through hole and a second through hole, and the right cantilever is provided with a third through hole and a fourth through hole; The first screw rod is connected to the right side plate after passing through the first through hole and the third through hole; the second screw rod is connected to the right side plate after passing through the second through hole and the fourth through hole.

4. A fully automatic nucleic acid extractor according to claim 1, characterized in that: The left cantilever comprises a first housing, and the third driving assembly is disposed in the first housing; the right cantilever comprises a second housing, and the fourth driving assembly is disposed in the second housing; The third driving assembly includes a third motor, a third coupling, a third screw and a third connecting block, wherein the third motor is fixed in the first housing, the third coupling connects the third motor and the third screw, and the third screw is horizontally arranged in a direction perpendicular to the guide rail; the third connecting block is sleeved on the third screw, and the third connecting block is fixedly connected to the automatic pipetting arm; The fourth drive assembly includes a fourth motor, a fourth coupling, a fourth screw rod and a fourth connecting block. The fourth motor is fixed in the second housing. The fourth coupling connects the fourth motor and the fourth screw rod. The fourth screw rod is arranged parallel to the third screw rod. The fourth connecting block is sleeved on the fourth screw rod, and the fourth connecting block is fixedly connected to the reagent kit grabbing robot.

5. A fully automatic nucleic acid extractor according to claim 4, characterized in that: The automatic pipetting arm comprises a left connecting block, a left mounting plate, and a left up-and-down motor, a left lifting screw, a left vertical guide rail, a left connecting seat and an automatic pipetting assembly mounted on the left mounting plate; the left mounting plate is vertically arranged and fixedly connected to the third connecting block through the left connecting block; The left lifting screw is vertically arranged and connected to the left up-and-down motor, and a left lifting nut is sleeved on the left lifting screw; The left connecting seat is slidably connected to the left vertical guide rail via a left sliding block, and the left lifting nut is fixedly connected to the left connecting seat; The automatic pipetting assembly is fixedly connected to the left connecting seat, and is used to take the pipette tip placed on the tip placement rack; absorb the reagent placed on the reagent tank mounting rack, and inject the absorbed reagent into the reagent reagent kit on the reagent kit mounting rack through the injection mechanism in the automatic pipetting assembly.

6. A fully automatic nucleic acid extractor according to claim 4, characterized in that: The reagent kit grabbing manipulator comprises a right connecting block, a right mounting plate, and a right up-and-down motor, a right lifting screw, a right vertical guide rail, a rotating motor mounting seat, a rotating motor, a fifth coupling, a mechanical finger mounting seat, a mechanical finger and a gripping hand assembly mounted on the right mounting plate; The right mounting plate is vertically arranged and fixedly connected to the fourth connecting block through the right connecting block; The right lifting screw is vertically arranged and connected to the right up-and-down motor, and a right lifting nut is sleeved on the right lifting screw; The rotating motor mounting seat is slidably connected to the right vertical guide rail via a right slider, and the right lifting nut is fixedly connected to the rotating motor mounting seat; The rotating motor is mounted on the rotating motor mounting seat, the robotic finger mounting seat is connected to the rotating motor via the fifth coupling, the robotic finger is mounted on the robotic finger mounting seat, and the clamping hand assembly is mounted on the robotic finger; the robotic finger is used to control the opening and closing distance of the clamping hand assembly according to the size of the reagent kit on the reagent kit mounting rack to achieve clamping and placement of the reagent kit.

7. A fully automatic nucleic acid extractor according to claim 6, characterized in that: The right up-down motor is mounted on the right mounting plate through an up-down motor mounting seat; The right up-and-down motor is provided with a first positioning sensor, and the rotating motor mounting seat is provided with a first limiting piece. The first positioning sensor is used to detect whether the first limiting piece rises to a specified position.

8. A fully automatic nucleic acid extractor according to claim 7, characterized in that: A second positioning sensor is arranged on the mechanical finger mounting seat, and a second limiting plate is arranged on the fifth coupling. The second positioning sensor is used to detect whether the second limiting plate is rotated to a specified position.

9. A fully automatic nucleic acid extractor according to claim 1, characterized in that: The heating and mixing assembly comprises a mounting base, a vibration motor, an eccentric bearing, a vibration element, a heating base, a heat sink and a fan; The vibration motor is mounted on the mounting base, and its rotating shaft is arranged vertically upward; the eccentric bearing is sleeved on the rotating shaft; the vibrating member is sleeved on the eccentric bearing to generate vibration on a horizontal plane driven by the vibrating member; an eccentric flyweight is also eccentrically sleeved on the eccentric bearing; The heating base is installed above the vibrating member and is fixedly connected to the vibrating member; the heat sink is arranged below the heating base, and the fan is arranged on the side of the heat sink.

10. A fully automatic nucleic acid extractor according to claim 1, characterized in that: The cleaning tank includes a tank body, in which an outer wastewater tank and an inner cleaning tank are formed, and the tank wall height of the inner cleaning tank is lower than the tank wall height of the tank body; a drain outlet is provided at the bottom of the outer wastewater tank, and a water inlet is provided at the bottom of the inner cleaning tank.

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